LOCUS       DACIFS010000001       107513 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.001, whole genome shotgun sequence.
ACCESSION   DACIFS010000001 DACIFS010000000
VERSION     DACIFS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 107513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.001"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            24..752
                     /locus_tag="IYA83_000001"
     CDS             24..752
                     /locus_tag="IYA83_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8585473.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(821..2344)
                     /locus_tag="IYA83_000002"
     CDS             complement(821..2344)
                     /locus_tag="IYA83_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ8585474.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            2596..3777
                     /locus_tag="IYA83_000003"
     CDS             2596..3777
                     /locus_tag="IYA83_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ8585475.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3837..5465)
                     /gene="treC"
                     /locus_tag="IYA83_000004"
     CDS             complement(3837..5465)
                     /gene="treC"
                     /locus_tag="IYA83_000004"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ8585476.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5916..8627)
                     /locus_tag="IYA83_000005"
     CDS             complement(5916..8627)
                     /locus_tag="IYA83_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ8585477.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(8637..9338)
                     /locus_tag="IYA83_000006"
     CDS             complement(8637..9338)
                     /locus_tag="IYA83_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585478.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            9524..10123
                     /locus_tag="IYA83_000007"
     CDS             9524..10123
                     /locus_tag="IYA83_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ8585479.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            10204..11217
                     /locus_tag="IYA83_000008"
     CDS             10204..11217
                     /locus_tag="IYA83_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8585480.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            11325..12242
                     /gene="rbsK"
                     /locus_tag="IYA83_000009"
     CDS             11325..12242
                     /gene="rbsK"
                     /locus_tag="IYA83_000009"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ8585481.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            12270..12665
                     /gene="rbsD"
                     /locus_tag="IYA83_000010"
     CDS             12270..12665
                     /gene="rbsD"
                     /locus_tag="IYA83_000010"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ8585482.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            12665..14038
                     /locus_tag="IYA83_000011"
     CDS             12665..14038
                     /locus_tag="IYA83_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585483.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            14395..16539
                     /locus_tag="IYA83_000012"
     CDS             14395..16539
                     /locus_tag="IYA83_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ8585484.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(16591..17631)
                     /locus_tag="IYA83_000013"
     CDS             complement(16591..17631)
                     /locus_tag="IYA83_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ8585485.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17908..19710)
                     /locus_tag="IYA83_000014"
     CDS             complement(17908..19710)
                     /locus_tag="IYA83_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585486.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(19724..20476)
                     /locus_tag="IYA83_000015"
     CDS             complement(19724..20476)
                     /locus_tag="IYA83_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585487.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(20485..20847)
                     /locus_tag="IYA83_000016"
     CDS             complement(20485..20847)
                     /locus_tag="IYA83_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ8585488.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(21023..21895)
                     /locus_tag="IYA83_000017"
     CDS             complement(21023..21895)
                     /locus_tag="IYA83_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ8585489.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21946..22434)
                     /locus_tag="IYA83_000018"
     CDS             complement(21946..22434)
                     /locus_tag="IYA83_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ8585490.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(22572..23402)
                     /locus_tag="IYA83_000019"
     CDS             complement(22572..23402)
                     /locus_tag="IYA83_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ8585491.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(23418..24026)
                     /locus_tag="IYA83_000020"
     CDS             complement(23418..24026)
                     /locus_tag="IYA83_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ8585492.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(24019..24336)
                     /locus_tag="IYA83_000021"
     CDS             complement(24019..24336)
                     /locus_tag="IYA83_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ8585493.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(24492..25220)
                     /locus_tag="IYA83_000022"
     CDS             complement(24492..25220)
                     /locus_tag="IYA83_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ8585494.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            25379..26260
                     /locus_tag="IYA83_000023"
     CDS             25379..26260
                     /locus_tag="IYA83_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8585495.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(26497..27540)
                     /locus_tag="IYA83_000024"
     CDS             complement(26497..27540)
                     /locus_tag="IYA83_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8585496.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27543..28211)
                     /locus_tag="IYA83_000025"
     CDS             complement(27543..28211)
                     /locus_tag="IYA83_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8585497.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            28353..30689
                     /locus_tag="IYA83_000026"
     CDS             28353..30689
                     /locus_tag="IYA83_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8585498.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(30804..31115)
                     /locus_tag="IYA83_000027"
     CDS             complement(30804..31115)
                     /locus_tag="IYA83_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585499.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            31296..32771
                     /locus_tag="IYA83_000028"
     CDS             31296..32771
                     /locus_tag="IYA83_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ8585500.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            32818..33402
                     /locus_tag="IYA83_000029"
     CDS             32818..33402
                     /locus_tag="IYA83_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ8585501.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            33404..33823
                     /locus_tag="IYA83_000030"
     CDS             33404..33823
                     /locus_tag="IYA83_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ8585502.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(34104..34388)
                     /locus_tag="IYA83_000031"
     CDS             complement(34104..34388)
                     /locus_tag="IYA83_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ8585503.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(34740..35450)
                     /locus_tag="IYA83_000032"
     CDS             complement(34740..35450)
                     /locus_tag="IYA83_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ8585504.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(35447..36919)
                     /locus_tag="IYA83_000033"
     CDS             complement(35447..36919)
                     /locus_tag="IYA83_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ8585505.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            37057..37530
                     /locus_tag="IYA83_000034"
     CDS             37057..37530
                     /locus_tag="IYA83_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8585506.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(37683..38234)
                     /locus_tag="IYA83_000035"
     CDS             complement(37683..38234)
                     /locus_tag="IYA83_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ8585507.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            38339..38869
                     /locus_tag="IYA83_000036"
     CDS             38339..38869
                     /locus_tag="IYA83_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ8585508.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(39352..39795)
                     /locus_tag="IYA83_000037"
     CDS             complement(39352..39795)
                     /locus_tag="IYA83_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8585509.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(39811..40971)
                     /locus_tag="IYA83_000038"
     CDS             complement(39811..40971)
                     /locus_tag="IYA83_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ8585510.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            41478..43178
                     /locus_tag="IYA83_000039"
     CDS             41478..43178
                     /locus_tag="IYA83_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585511.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(43280..44113)
                     /locus_tag="IYA83_000040"
     CDS             complement(43280..44113)
                     /locus_tag="IYA83_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ8585512.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(44417..46582)
                     /locus_tag="IYA83_000041"
     CDS             complement(44417..46582)
                     /locus_tag="IYA83_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585513.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            46842..49136
                     /locus_tag="IYA83_000042"
     CDS             46842..49136
                     /locus_tag="IYA83_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ8585514.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            49129..49794
                     /gene="pgmB"
                     /locus_tag="IYA83_000043"
     CDS             49129..49794
                     /gene="pgmB"
                     /locus_tag="IYA83_000043"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ8585515.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            49787..50794
                     /locus_tag="IYA83_000044"
     CDS             49787..50794
                     /locus_tag="IYA83_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ8585516.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            50816..51835
                     /locus_tag="IYA83_000045"
     CDS             50816..51835
                     /locus_tag="IYA83_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8585517.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(52116..53483)
                     /gene="nhaC"
                     /locus_tag="IYA83_000046"
     CDS             complement(52116..53483)
                     /gene="nhaC"
                     /locus_tag="IYA83_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ8585518.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(53592..55013)
                     /locus_tag="IYA83_000047"
     CDS             complement(53592..55013)
                     /locus_tag="IYA83_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8585519.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(55256..57133)
                     /gene="tdc"
                     /locus_tag="IYA83_000048"
     CDS             complement(55256..57133)
                     /gene="tdc"
                     /locus_tag="IYA83_000048"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ8585520.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(57424..58680)
                     /locus_tag="IYA83_000049"
     CDS             complement(57424..58680)
                     /locus_tag="IYA83_000049"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ8585521.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            59260..60561
                     /locus_tag="IYA83_000050"
     CDS             59260..60561
                     /locus_tag="IYA83_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ8585522.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(60630..61229)
                     /locus_tag="IYA83_000051"
     CDS             complement(60630..61229)
                     /locus_tag="IYA83_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8585523.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            61483..62364
                     /locus_tag="IYA83_000052"
     CDS             61483..62364
                     /locus_tag="IYA83_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585524.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            62377..63987
                     /locus_tag="IYA83_000053"
     CDS             62377..63987
                     /locus_tag="IYA83_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ8585525.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(64064..64723)
                     /locus_tag="IYA83_000054"
     CDS             complement(64064..64723)
                     /locus_tag="IYA83_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ8585526.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(65006..65671)
                     /locus_tag="IYA83_000055"
     CDS             complement(65006..65671)
                     /locus_tag="IYA83_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585527.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(65818..66423)
                     /gene="dhaL"
                     /locus_tag="IYA83_000056"
     CDS             complement(65818..66423)
                     /gene="dhaL"
                     /locus_tag="IYA83_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ8585528.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(66451..67434)
                     /gene="dhaK"
                     /locus_tag="IYA83_000057"
     CDS             complement(66451..67434)
                     /gene="dhaK"
                     /locus_tag="IYA83_000057"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ8585529.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(67436..67831)
                     /gene="dhaM"
                     /locus_tag="IYA83_000058"
     CDS             complement(67436..67831)
                     /gene="dhaM"
                     /locus_tag="IYA83_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ8585530.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67985..68305
                     /locus_tag="IYA83_000059"
     CDS             67985..68305
                     /locus_tag="IYA83_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ8585531.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(68417..69286)
                     /locus_tag="IYA83_000060"
     CDS             complement(68417..69286)
                     /locus_tag="IYA83_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ8585532.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(69382..70686)
                     /locus_tag="IYA83_000061"
     CDS             complement(69382..70686)
                     /locus_tag="IYA83_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8585533.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(70683..71192)
                     /locus_tag="IYA83_000062"
     CDS             complement(70683..71192)
                     /locus_tag="IYA83_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585534.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(71367..73502)
                     /locus_tag="IYA83_000063"
     CDS             complement(71367..73502)
                     /locus_tag="IYA83_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8585535.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(73585..75240)
                     /locus_tag="IYA83_000064"
     CDS             complement(73585..75240)
                     /locus_tag="IYA83_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ8585536.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(75242..75556)
                     /locus_tag="IYA83_000065"
     CDS             complement(75242..75556)
                     /locus_tag="IYA83_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ8585537.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            75726..75986
                     /locus_tag="IYA83_000066"
     CDS             75726..75986
                     /locus_tag="IYA83_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ8585538.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            76000..76314
                     /locus_tag="IYA83_000067"
     CDS             76000..76314
                     /locus_tag="IYA83_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ8585539.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(77044..77598)
                     /locus_tag="IYA83_000068"
     CDS             complement(77044..77598)
                     /locus_tag="IYA83_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ8585540.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(77724..79653)
                     /locus_tag="IYA83_000069"
                     /pseudo
     CDS             complement(77724..79653)
                     /locus_tag="IYA83_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(79959..80675)
                     /locus_tag="IYA83_000070"
     CDS             complement(79959..80675)
                     /locus_tag="IYA83_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ8585541.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(80676..82499)
                     /gene="glpO"
                     /locus_tag="IYA83_000071"
     CDS             complement(80676..82499)
                     /gene="glpO"
                     /locus_tag="IYA83_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ8585542.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(82513..84009)
                     /gene="glpK"
                     /locus_tag="IYA83_000072"
     CDS             complement(82513..84009)
                     /gene="glpK"
                     /locus_tag="IYA83_000072"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ8585543.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(84145..85605)
                     /locus_tag="IYA83_000073"
     CDS             complement(84145..85605)
                     /locus_tag="IYA83_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8585544.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(85595..86890)
                     /locus_tag="IYA83_000074"
     CDS             complement(85595..86890)
                     /locus_tag="IYA83_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ8585545.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(87222..87899)
                     /locus_tag="IYA83_000075"
     CDS             complement(87222..87899)
                     /locus_tag="IYA83_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ8585546.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87929..89020)
                     /locus_tag="IYA83_000076"
     CDS             complement(87929..89020)
                     /locus_tag="IYA83_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ8585547.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(89121..89555)
                     /locus_tag="IYA83_000077"
     CDS             complement(89121..89555)
                     /locus_tag="IYA83_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8585548.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(90139..91596)
                     /gene="ascB"
                     /locus_tag="IYA83_000078"
     CDS             complement(90139..91596)
                     /gene="ascB"
                     /locus_tag="IYA83_000078"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ8585549.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(91593..93488)
                     /locus_tag="IYA83_000079"
     CDS             complement(91593..93488)
                     /locus_tag="IYA83_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585550.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(93546..95321)
                     /locus_tag="IYA83_000080"
     CDS             complement(93546..95321)
                     /locus_tag="IYA83_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8585551.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(95371..96867)
                     /locus_tag="IYA83_000081"
     CDS             complement(95371..96867)
                     /locus_tag="IYA83_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8585552.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLYYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(97061..98683)
                     /locus_tag="IYA83_000082"
     CDS             complement(97061..98683)
                     /locus_tag="IYA83_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ8585553.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(99011..100324)
                     /locus_tag="IYA83_000083"
     CDS             complement(99011..100324)
                     /locus_tag="IYA83_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ8585554.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(100569..100793)
                     /locus_tag="IYA83_000084"
     CDS             complement(100569..100793)
                     /locus_tag="IYA83_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585555.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100813..101181)
                     /locus_tag="IYA83_000085"
     CDS             complement(100813..101181)
                     /locus_tag="IYA83_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ8585556.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(101377..102087)
                     /locus_tag="IYA83_000086"
     CDS             complement(101377..102087)
                     /locus_tag="IYA83_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585557.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(102105..102602)
                     /locus_tag="IYA83_000087"
     CDS             complement(102105..102602)
                     /locus_tag="IYA83_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8585558.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(102639..103091)
                     /locus_tag="IYA83_000088"
     CDS             complement(102639..103091)
                     /locus_tag="IYA83_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8585559.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(103226..103831)
                     /locus_tag="IYA83_000089"
     CDS             complement(103226..103831)
                     /locus_tag="IYA83_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ8585560.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(104281..104901)
                     /locus_tag="IYA83_000090"
     CDS             complement(104281..104901)
                     /locus_tag="IYA83_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8585561.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104903..105772)
                     /locus_tag="IYA83_000091"
     CDS             complement(104903..105772)
                     /locus_tag="IYA83_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ8585562.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105795..106202)
                     /locus_tag="IYA83_000092"
     CDS             complement(105795..106202)
                     /locus_tag="IYA83_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ8585563.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(106221..107510)
                     /locus_tag="IYA83_000093"
     CDS             complement(106221..107510)
                     /locus_tag="IYA83_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8585564.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        31545 a        22373 c        18877 g        34718 t
ORIGIN      
        1 aaataaaata aaggagtaat gatatgattc ccatttatat ttgtgaagac gatccaaaac
       61 aattagaagt tttatcaacg gtgatcaaaa atagaatcat gattgaaaac ttagacagtt
      121 atgtccatat ttctacctct gatccggaag aacttctcaa tgcagccaag atcagaactt
      181 cttcttttgg tatttacttt ttagatatcg aactagaaga cagtgaaatg gatggtgtag
      241 cgatcggtaa attgatccgt gaattagatc ctttaggaaa aataatctat gtgacttccc
      301 atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga accgactgat tatatcgtca
      361 aagaagatct ttatgattta aaagaaaatg tcgagcatat cctgagcaaa attttcgaag
      421 attttcaaat gtcccaaatc caaaaagata tcttcaaaat agagtttaat gatgagatca
      481 aatttttacc gatcaaagac atccaatatt tttctacttc gctaggagcg ccacataaac
      541 tggaagtcca tttgactcat gcccaattac aattctatga gaagatcaaa gaaatcgaga
      601 aaatgcatcg gaattttatc cgttgtcata aatcctatgt tatcaataca caaaatgtgc
      661 ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa cggcgagacc atcccagcct
      721 ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc gaatagtttg gaccgactaa
      781 atgaccagcc gatccaaacc cttcggttct tttttgccta tcagtgcaaa agcatttcct
      841 gtgcaatttc acgtccatcc attgtatctg gataaaatgt tggccaattc tccatttctt
      901 tcagcaatac ttcttgactt tcatttcctg catataaatg aaaatgttcg gagtcagccg
      961 gtgcaatcgt gtgatcactg aattgaatat atctaaaagc gccactttcc ttatcttctg
     1021 cttcgaatag ataccttacg ccacggtttc ctgcctcgta ggtcaagatt tctttcccta
     1081 cataacgata atttgaacgt ttcgttgttc cattcttaaa cgtaaatgcc atctctttct
     1141 cactgatctt gattgaagca atatctgttt gatagccctt cgtataatat tctttatatt
     1201 cttgtgctgt cttatcctga ttcaattttt ctttatattc aaaaacagga tcaaaagtcc
     1261 catcttctac atacgtatct actgtttgcc aagttcctgc ccaatctgaa agagagcgat
     1321 cttgtacatc ctcgtcttca aaataaccgt tgtagacgct cttttctttt ttgggggtca
     1381 aagtatcttc tagcgggttt tctgtctctg tcgttttttt caacgcttcg aggtttgctt
     1441 ccatgataga aagatagttt tctccatttt ctatttgttc attcgtcaat ccttccaaag
     1501 gattcaatac ttctaaagat acacctgctt cgtttgctaa cgttctggca atactatctt
     1561 tggcgttttc ttcaaaataa atatagttga ttccatgttc tttaacaaag tgtttcagtt
     1621 cagcaattct cgccgcagac ggttcttcac tagaagaaag accagcaatc gatacttgat
     1681 tcaatccata ttctaacgct agatagttga atgcagcatg ttgcgtgaca aaagatcttt
     1741 gttttgcttg cttaaaggtt tcttgataaa gctgatggag tgcttgaagt ttctcggtat
     1801 aggctgctgc attttttgtc catacttcct gtttttctgg gtaagcttca atcagctgat
     1861 cacgtatgtt tgctacctgc ttgattgcta aacttggagc tagccataca tgtgggtcta
     1921 gctcatgtgt atggccttct tccccgtgtt catggtcatg atcatgtcct tcttcacttc
     1981 ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg gacattgcct tcttgtaatg
     2041 ttttttggat tgcggggacc catgtttcca tgttttcatc attataaaca aagatatctg
     2101 tatcttggat ctttgccatg tctttagcag aaggttcata atcgtgggaa tcagtccctg
     2161 ctggaatcaa taactctact tctccttcat ccccgacgat tgcttttgta aaatcataca
     2221 tgggataaaa ggtcgtcatc acgctcaatt tttctttact gtcctcggct ttcccacttg
     2281 ccccgcaagc agttaacata cctgctaaaa ccagcatggc caatatacct atcaatcgtt
     2341 tcatttatga cactcctctt ctcatcttct ttcatttgaa tgttccgatt cgcttttcgc
     2401 aaaccgtaat agttacgttt tagcaacagg atttaattta tcatatggtt aaaagaactg
     2461 tcaagcgttt tcttatttta attgatgaga aagtttatat gaatgattga cgatattttt
     2521 gcaatcaacc tctatcattt tccttttata cccgcttttg tctataatga agttatagat
     2581 aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga atcgtttgat ccgcttttta
     2641 ggaggaaaaa cctcttacta tgttttagga atagttatct tacttgcgct gatcatttat
     2701 tttttccatc agatctcgtt tatattcaag cctgtcggaa tcattatcgc tactatcttg
     2761 cccccattat tgtttgcact gatcttgtat tatcttttgg aaccagtcat caaacgtttg
     2821 agcaagcacc tttcgaggaa cttgtcagtc attatcgttt atgtgattat tttattttta
     2881 ttggggcttg gaagcatatg gcttattcct tttcttgaag aacaggcaaa gacattgatc
     2941 agtagtttgc cagatttgtt tcaagatttt caagagagtc tccgacaatt tttggagaaa
     3001 acaccttttg ccgattcatt taatcaattt ttcgcttcta ttgacgacgt gacgtctgat
     3061 atagctggat ttattggaaa ttattgggaa agtggcgcac aacggatcag caacattttt
     3121 tcaactgtta cagctatttt tattacttta tttactggtc caattgtcgc ttttttcctt
     3181 ttgagagatc ctcaaaaatt ttatcagtca gttttagcga ttgtcccacc agcatttcgt
     3241 acagatttta aaaatttgac aaagattgct aatcaacaat tgggttcatt tctaaagggg
     3301 caaatcattg cttcatttat ccttggagca gtctactggg tttgtttctt actgatcgga
     3361 ttagaatttg ctagtgtttt agctatttct gctgggttgt tatgtatcat tccatatatt
     3421 gggccgttta tcgccttctt tccaggtttg atcattgcat tccaagactc tacttttatg
     3481 gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc tccacggtga cttggtgatt
     3541 cctcgagtaa tgggtgatcg tttacagatc catccgatta cgatcttaat cgttttactt
     3601 gtcatgggtg atctaatggg aattgtgggc gttatttttg gtattcctat ctatacgtta
     3661 gtcaaattac tcgttacttt tattttccgt aaattcaaac aacgctacaa taaattttat
     3721 ggcgataaag gagagtatga aaactcttca ttttctgaag aagattattt taaataaaac
     3781 acaaaacgtc gatcttacga gaatagctcg taggaacgac gttttgtgtt tttgttttaa
     3841 tttttcagca aagcaattgc ctggtatggt tttagctcca gactttgatg aagaacagta
     3901 tcggaataat tatcgataag aatattggct tctgtgaatc cttctgggat ggcgatcctt
     3961 gtatctttat catagaaatt tgttaataca agcagctgct gtccttcaaa ttggcgaaga
     4021 aagccataaa cttccggatg atttgcttca taagcatgat aatctccttg tgcaacgatt
     4081 ggatatgttt ttctcagccg aatcaatttc ttgtaatagg acaaaataga atcttcagaa
     4141 gatagttctt tttctacatt aatctcttct agcttgcctc caacttttaa ccaaggtttc
     4201 cctgtagtaa atcctgcttg ttctgtgtct gaccattgca tgggtgttcg agagttatcg
     4261 cgagattttg ctttgatccg acgaaacgct tctttttctg tgaatccttg ttcaaggagt
     4321 aacttataag cattcaaact ttctacgtca atatagtcgg aaatcgatga atagtctggg
     4381 tcgatcatgc cgatttcttc tcccatataa atatagggcg tcccacggtt caaatggatc
     4441 atggttgcta gcatctctgc tccttgtttt cgatactgat cgatagctac aaatcgattg
     4501 agcgcacgtg gctgatcatg attattaaaa aatagagcat tccagccgtt accctcactc
     4561 atcttttctc cccatgtatg aaatagtcgc tttaattctg caaaatcaaa tggaggagta
     4621 gaccatttat tcccattttc ataatcgact ttcaaatgat ggaaattaaa gaccattgat
     4681 aattcttctc tactaggttc tgtatacaag atacaatttt cgatcgtcgt agagctcatt
     4741 tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg cttggttcaa ttcttttaga
     4801 tacgtatggg tgattggttt gtccgtatat tcaggtttgc catcattttc tttattattt
     4861 ttcaattgtt cgtctttacc gataacattg ataacatcga aacggaatcc ttttacccct
     4921 ttttccatcc aaaaacgaac aactttgaat agttcctctc ttactgcttg gttccgccag
     4981 ttcaagtcag cttgtgtccg atcatataag tgcaaataat atttccccgt atctccaaat
     5041 ggcgaccaag cacttccacc aaattttgat acccaatccg ttggctcttc tcttaaaatg
     5101 aagaagtcct gataatgctg gtcccctgct agtgcttttt tgaaccattc atgttctgtc
     5161 gaaacgtgat tcagcaccat atcgagcatg atttctatcc ggtatttctt tgcttcggct
     5221 attagctgtt cgaattcctc catcgtacca aagataggat cgatggctgt gtaatcagag
     5281 atatcataac cgttgtcttt ttgagggctt ggataaatag ggtttagcca aatcatgtcg
     5341 atgcctagat cattcagata cgggagtttt tctttgattc cttttaaatc tccaattcca
     5401 tcaccattac tgtctttgta agatttggga tagatttggt aaatgacttt gtctttaaat
     5461 gtcatggata tttacctcca ttcaactgat ttattaccta ccacaactta tatatacaag
     5521 ttatcattta ttattataac tgaaccgaaa gcgtttgtaa aatttatctt tatacattct
     5581 aagaggtctg ttgcccaata aaaaacaaga gatcagacat gtatttgtct atcctcttgt
     5641 ttaaaatagc aaaatattct tgtttcttct ataaatggtg ttcaaaagct tttatctacg
     5701 gcaatcagtc ttctcaaatg taaaacatga ggagctaatt ttatttgagt attctttttg
     5761 ctcgatgcag gacggctttt ttacaacctc tgattcatgg tgttcaatca gctgtccttc
     5821 ggtattagct caaaaaacgg caaaatatga aaaactattt tgccgtttgt ttctctaata
     5881 ctcaggtcaa accgctgatt tcacaacctc tgtttttatt cattcgattt caacgcatca
     5941 atcatatcga tatgctttaa tttgaaatgg gtaacaatca tcacaataac cgtaaatacg
     6001 atagttaatc ctgctgataa gacataagcc caccatgtga tcactaatgg aaagatgaca
     6061 ttctccatag acgcttgttg caagatgaag tcagtcaata tatatcctac accaaatcct
     6121 ccgatgattc ctaaaagagt aaagatgata ttttctcgaa caatgtacat cgtgacttct
     6181 ttatcaaaaa atccaaggac ttttattgta gataattcgc gtacacgctc ggaaatattg
     6241 atattagtta gattatacaa gacgataaaa gccagcagtc ctgataatac gacaaagatt
     6301 aagacaaccg agtctaagtt cgccattgat tcttcttgcg tttcgatttg tttggacatg
     6361 aaagacgtat ttgtcacagc gtctgtggca agcagtttct cggaaagcat ttcttcttct
     6421 tttgttgtca tcttatctgt acgcagcaaa aatgcattat ccatcatcga ttgatcagta
     6481 actgaagcat aatattcagg ggacatataa ataaagctac ctagatagtt tttcacgatt
     6541 tcttttactt cgactttcca ttctttttga tcttcatcat aaagcgaaat atgatctcct
     6601 gtagataagt caaatagttc tgctactttt tgcgtaatag cgattcctgt tttaggcaag
     6661 gagcgtgggt tctcttcaag atcagttaaa tggataaaac gatccaaatc agctgtttta
     6721 cttggtacga tcactgtcag actttgcgaa gcatgtcctt tttttctcaa ttctacggtt
     6781 tgagtatgaa tagctaggat ctcatttact ttttcttgct tctgcagcat atccgctgct
     6841 ttttgacgac tatcttccgt ggatgaatcc aatgtaacga ttgcttgata gtcaatgatc
     6901 ggtccaaatt gtttggctga aacagaactt agagaatctt tcaatcccac gcctgcaacc
     6961 atcagcccgg cacatccagc aattccgata atcgccataa gcattcttga cttatatcgg
     7021 aagagatttc ggtaactgat tttttgatta aagctaaggc gtgaccacag cggagtgatg
     7081 tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg gttgaagcaa tgcagcaggc
     7141 cgttctctta aatctttgaa taaaacgatc attgcagcgc caaatgcggc aatgaagaat
     7201 gcggcagctg cttgtaaaat cggcggccag tcataaaaaa taagcgcact cccaatatca
     7261 taccgctcgt tagacagctc aaaaatgatc cttggcaaaa gattcgttcc taatatcgtt
     7321 cctaaaacaa tacctatccc tgaggctaga cttgcataga ttgcatattt ttttgcgatt
     7381 tcaactttcg tataacctaa cgcctttaat gtcccgattt ccctgcggtt ttcttctacc
     7441 atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa agaaaaagaa aacaggaaaa
     7501 acattggcaa tcgcagcgat acgatcagac agatcaccaa attcttgaaa accgacattt
     7561 tctgaacgtt cattgtatag gtatgatggt ttagttaaat tttcaatttt ttctgcattt
     7621 cgattcaatt cttcctctgc agaggccagt tcacgttctt tctcaactaa ttgcctttct
     7681 tgttcagcaa gttgagcttc ttgttgagat aatgtttgaa cagcgattgt tcgttgcgct
     7741 tcaggaagct gctggatagt tgctgactgt tgcgctaact gttttttagc atcttctaac
     7801 cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta ttttttgctg tgccggttct
     7861 aattcttcgt tggccgattt tttcagctct tctaagcgtt cttcgggtcg atcttgtaaa
     7921 tacttttctg ccttttcttg attttgctcc attttatctt tgtaagcttt gctgtacgta
     7981 ttcaaattct ttacgtttgt aaaatcaaga tataagacag aataaatatc tgttttaaaa
     8041 acggattcag gtaaatagac aaaataatcg acgctaccat ttccgacgtt cgcgtatcct
     8101 ctctccaaat tgttgatgaa caatggagaa cggacaaagc ccgtgatggt atactctttt
     8161 tctttcaatt catctgaatc aatctgataa atatcaccga ttttataccc ttcatcttcc
     8221 gctttttcat ccaatacaag ctggtttggt ttttccggga gttttccatc agttaattgt
     8281 agcttgttct gcttgttatt tttgttataa gaaaatattt gaatcacttc gtttttgcca
     8341 gtattggaat aatagatctg ttttcctgtt tctacttttg catctgggat cttttctgct
     8401 tgagcaatat ctttttgtgt caagccacca gtagaaacga tttgcaaatc agaaagtcct
     8461 gctgtaccaa catagtgatc catcgttttt tgcatgacag gtccagcagt tttgacacct
     8521 acaaataaaa gtgttcctag catgacaata aggatgattg caatgaatcg tcctttagat
     8581 tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg ttttcataaa atccccctac
     8641 catacaatct catcaatgga tgaaggatga tcatttaatt caacagacct tactttagca
     8701 tcattaatgt gaatgactct atcagcgatg ggcgctaaag cagaattatg tgtaatgatc
     8761 aaaacggtat tgccgtgttg tctgctagcg ttttgcaata atttcaatac ttgcttgccc
     8821 gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa gaagtttggg atttttagct
     8881 agtgcacgag caattgaaac cctctgctgt tcgcctcctg ataattggga cggaaagtta
     8941 ttcaatcgat gagctaggcc cacttgcctc aatacctcta caggatcaag cgcatcagga
     9001 gaaacttccg ttgccaattc cacattttct tttgctgtta gattaggaac gagattgtag
     9061 aattggaaaa cgaaccctac atctgttcgt cggtaagcag taagctgttt atctgaaaat
     9121 tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat caggtgtatc catcccacct
     9181 aaaatattaa gaatcgttga ctttcctgct ccacttggac caagtataat cactaattcg
     9241 cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa tggtcgtttc tcccattttg
     9301 tatcgtttgt attcattttt tacttcgaca tacttcactc tgattcctcc attttcacga
     9361 tatttctcgc tatatctatg catcgaatgc gttttctcac acttatttat tttaccactg
     9421 aatttctcta gactaaagaa aggaatcctt taatttagtt gacaaataaa cgatttatga
     9481 tttacttaca taaaaaacat tgttatttga aagaaggaac atcatgaatg atttaaagga
     9541 aaatatgatt tttgatttat caaaaactgc ttttgtcgcg atcgacatgc aaaaagagat
     9601 cgtaaataac ggtacactct ctccacacac tgcagcatct attctgacaa acaatgattt
     9661 gttagtaaga acattgaaac gtaccgctgc cttgaaagtt ttagtgaacg tagacatctc
     9721 cactttccct tatttgagtc aacaaactga tatgggtgga atgactgctt ctgtacctcc
     9781 agaattcact gatttacttt tgaaggattc tttgaaagat acagataata tgttgactat
     9841 cacaaaatac aatccaagtg ctttttttgg tacttctctt gatcttcaac ttcgccggcg
     9901 tggaatcgaa actatcattt taagcggtgt agcgacaaca aacggtgtat atgctactgc
     9961 attagatgcc tttcaacacg ggtaccatat cgtgcttgct gaagatgctt gcagtgatcg
    10021 tgacaaagaa tcccatcaat tgttcataaa aaagatattt ccgaaaactg cacgagtacg
    10081 ctcaacgaaa caaataatcg aagctatcca acaaagcaaa taagtactgg cacacgtgat
    10141 tttcatttac ttctttttga tttcatggga aaataaccac aggatttaga taggagtatg
    10201 aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta aactttccgg cgtttcaatc
    10261 acaactgtct ccttgatatt aaatggaaat acggagaaat tcagtccgaa aactgttcag
    10321 aaagttttag ctgccaaaga agaattaggc tatcagcctg actattttgc tagacaaatg
    10381 attaccaagg aaacgaaaac aattggtgta ctcgttcctg atatcacgaa tccattcttc
    10441 agtacactga tgcgaggaat tgaggatatc ttgtataagc agaatttcgt cacgatatta
    10501 tgtaatgctg attctgatca tcaaaaagaa atcgaatatc tagccgagct cacacgtcga
    10561 ggtgtggatg gattcattat cgcgacttct gctgtctcta cagatgcaat caacgaaaat
    10621 ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga aaaaatctga aggatttagt
    10681 gatgccgtac gaacagacga ttttcgcggt ggttacctag cgggcatgca cttgcttagt
    10741 ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc caccggaaaa tgtccacgct
    10801 cggattgagg ggtttaaatc tgctcttgat gtttaccaaa tccctcacga ccaactgatc
    10861 ctcttaccta cacagttctc aaaacaaggt ggctaccaga tcacagcaga acttcttgat
    10921 tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag cttttggatt atatcgcgga
    10981 ttagaggaag ctggaaaaag catccctgaa gactacagca ttattggcta tgataatata
    11041 gatatgtgcg agtatatcaa gccgaaactg acaacgatcg cacaaccaat cttcgaattg
    11101 ggacaaacat ctgccaagtt attgttagat cggatccaat tcccagaaaa agaatgggaa
    11161 gaaaaaagat tacctgtacg attagaaaaa agattttcaa ctgcaccctt gaaatagctt
    11221 tcaaggtgtg ctatattttt atcgaactat taaaacgttt tactaaaacg ttttaaaaaa
    11281 taattttccc atcaaaataa atgagatagt tggaggtttg taaaatgaac acggttacag
    11341 taattggaag tattaatatg gacactaccc ttcgtttaac caacatgccc aagccaggcg
    11401 aaacgatgca tgcgcatgag atttttcacg ctggcggagg taaaggtgcc aaccaagcag
    11461 tggcagccaa acgttcaggc gcacggacat cttttattgg aggcgttggg gctgacagcg
    11521 aaggacaaca gttattagat ctactgacaa aagaaaacat cgatacatct gggatagctg
    11581 aaatccaagg cgccacaacc ggacaagcaa tgattatggt agacgcagca ggagaaaata
    11641 gtatcttgat ccatgcaggg gcaaataatg ctttccatga acaagaagtc ctaaaaaaca
    11701 agcaactgat tacaaacagt gactttatta ttgctcagtt tgaaagtagc ttagatgcga
    11761 cgatcctagc tttttctatt gcaaaagacg caggaaaaac aacgatcctg aatcctgcac
    11821 cagctcgtga aacgattcct actgaactac ttgaaaagac agatatcatt ataccaaatg
    11881 aaacagaaac agaaatcatt acaggaatca gagtgacaga tcataacagt ttggtcgcag
    11941 cagccgaaaa actccatgaa ttaggtattg gaacagtaat cattacacta ggcagtgccg
    12001 gcgctttcta tcatacagaa aaagaacacg gtatcgtccc tgcttttaaa gtggatgccg
    12061 ttgacacaac agccgcaggt gatacattta ttggcgcatt aagcagcaca cttcagccag
    12121 accttagtaa cttgaaagaa gcgatccttt atggaaatct agcctcttct gttgctgtgc
    12181 agtcttatgg tgcgcaacct tctatccctt accgcgaagc tttagaacaa tcagcagtct
    12241 aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg aaaagtaatc aattcagata
    12301 tttcacgagt aatcgcacaa atggggcact ttgataaatt aagtatcggg gatgcaggca
    12361 tgcctgtccc ctttggtact gaaaaaatag atttggctgt ctcaaacggt cttccaagtt
    12421 ttttatctgt tttagaaaac gttttagaag aacttgaggt acaatcgatt gaattggcgg
    12481 aagaaataaa gacaatgaac ccggaaatat tggaaaaaat caaattattg ttaccagata
    12541 caccgattag ctttatccca catgaagaaa tgaaaaaaga gttgaataac tgtcatgcat
    12601 ttattcgtac aggcgaaatg acaccttacg ccaatattat attagtgagt ggagtggtct
    12661 tctaatggaa acgacaatga atcctgaaca gatggtactg tccgagcaga ttcatatgct
    12721 gattgaacag ttgggaggaa aagacaatat tgattctgtc cttccatgtg cgactcggct
    12781 tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa aaattaaaac agcaaatccc
    12841 ttccattaag tatttcattt tactcgatag atatgcagaa ataatcatgg atcagggatt
    12901 ggctcctgct tacaaggaat tgacgcgtca aatcgatcat tcagctccga aagaaaagct
    12961 aaatccgatc gaaaaactat ttggtttgat atcggaaatc tttacgccga tcattccgct
    13021 gtttgccggt tctggtatat taaaaggtct ggttgtttta gcgactacga ttggctggct
    13081 tagtgaaaca agcggaactt atcatatttt gtctgctgcg tcgaatgccg tcttttattt
    13141 tctgccaatc tttcttgctt tttctgctgc caaaacgttt aaagtgaatg gatatattgc
    13201 ggctgtcatt gctgcagcac tgattgaacc gaatctgacc tctttacttt cagacagtgg
    13261 caaagcagct actgattttc tggggattcc tgtagtactg atgcagtata cgtctactgt
    13321 catgccggcg attttaggta ttttctttta ttcttttgtt gaaaaattcc tcaaaaaata
    13381 tattcgcgaa aatatgcaat tagtatttgt acctttactt tcattagtta tcacggttcc
    13441 tttgacgtta atggtattcg ggcctgcggg tgtttacctt ggcgaaggtt tagctgccgc
    13501 aattacttgg atgatggata ttaacggacc gatcagtggc gcaatcatcg ctggtggttg
    13561 gaatattttg gtaatatttg gtatccaatg ggctgtaaac cctgtcatga tcagtaatat
    13621 ctctgcttat gggttcgacc gaatcgttcc gttgacagga gcagccaatt ttggaatggc
    13681 tggtgcagct ttaggtgtat ttctgcgaag taaacgttca aaaacccgtt cgatctccgg
    13741 ttctgctttt gcttcgattt tacttgcggg agtgaccgaa ccaactgttt acggaattgc
    13801 gatcccattg aaaaaaccat ttgttgctgc ttgcattggt gcagctgctg gtggagctgt
    13861 aatgggattt gcccaagtga aagcgatcgc ttttgtattt ggtagtctga ccacattgcc
    13921 tgcattcatt tccggtactt ttttctggta cttagcagga ctggctgtta gtctagtcgt
    13981 agcaatgatc actactctcg ttagcgggtt tgatgaagac ctaatgtctt atgaatagtt
    14041 aatgaaaaag actgaagtat gagatgttta tcacatattt cagtcttttt ctttttagga
    14101 tcattatttt tataaatttt tgtgatactt tccaaacttt ggtataattt acatatgcat
    14161 aaccattttt atccccactt gtttatcccc aaacaaaggt ggtctatgca ataaaatgaa
    14221 cgtcaaagaa aaacgaatga atgctcgttg cttatttaca aattgtttta tttagaatac
    14281 acagacagat ttatgaaaag aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc
    14341 taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa
    14401 aataaaaaaa cgatacgttt gctagctgct acttggctga ttgctccgtc tgttttgtcg
    14461 accaataccg tatttgccgt agataatcaa atagctgatc aaacagtcac aacagatacg
    14521 actacatcca gctcagctac tagtgaaaca acggattctt ttacagaaga cccaactgga
    14581 acaactaact caacaacagg atcgaccgag ccaactgaaa ttgatccggc tgatccatca
    14641 gaaaatgaaa agccagccga gacatattcc cttacaattg ctccttcttt gattggtaag
    14701 atccaactga ctgcttcgac agcggattcc cctgaacagc aacctttgga tattacggaa
    14761 agtggaacgg tagaaggttt attagcggaa acgaaggtaa cttacactat tacgccagct
    14821 gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca
    14881 aagaccttta cagtaggtac gaacaattta tctattactg cagaatttaa agctggaccg
    14941 gatacttcaa cagagaaacc aactgatcct agtgaagaag tccctagtac accagatccg
    15001 gaaacgccaa acaaaccaga tgtgggtaat aacaacaata ataatggttc aaataattca
    15061 aacaataata acaataattc gaacagcggg aattcttcga atcaaacaaa cccatctgga
    15121 tcgaataaca atggaaatac tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat
    15181 ggcggaacga acaatgcttc atctcgtcca aatacagcaa atcaaagcgg tatagagaat
    15241 ccatcttctg cttcttctga ttttgttgta aaatcaccaa ttgatgctgt attgcctaca
    15301 aatacaacga atgtccaaca agcaatcgtt cgagaagcat tcaagcatct tggaaaacca
    15361 tatgtatggg gagcaaaagg tccaaatact tttgactgtt ctggtttgac ttattacgta
    15421 tatatgaaag caaccggtca ttatatcggt ggctggacag gcgaacagca atatgcaggt
    15481 acgcagattc cagtaagtca ggcccaacct ggcgacctcg tcttctgggg accttcttcc
    15541 ggtgttactc accatgtagg tatctatatt gggaacggac aatttatcca tgcgcctcaa
    15601 ccaggagata aagttcgagt aacttctatt tcagatttca cgcctgattt tgctgtccga
    15661 gtaaacttag caggtttacc tgctgcttca ggttcgctga caggcggctc atctatttta
    15721 gatggattag atggctcgtt ccatttcagt caaaaccaaa caactgatca gttcttgaaa
    15781 aagattgcag atgatgcaca ggaaatcggg caaaaagaag gaatctacgc atcagtcatg
    15841 atggctcaag ctatccttga aagtggatct ggaaacagct tattatcaag cgaaccaaat
    15901 cataaccttt ttggtatcaa aggatcatat aaaggaagca gcgtgacctt caatacactt
    15961 gaacaagata gttccggaca aagctaccaa atccgcgcac aattccgtaa atatccttct
    16021 tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa atggactgac tggcaatcct
    16081 gatttctaca aaccaacttg gaagtcggaa acaaaagact acaaagaagc aaccaaatat
    16141 ttggaaggac gttatgctac agatcgccaa tattcacaaa aattgaacgc gatcatcgaa
    16201 gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca
    16261 aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc caatcaaatg gacaacagat
    16321 ggcgtgccaa tcacaggatt agaattttca acgataaaac gcgtcaacaa cgcttcttcc
    16381 atcttccgtg tcgcttcaat ctgggatttg tggaacaatt ttacggcaag acagatacca
    16441 gaaacaacta ctcgtacagt taaagctgca actgttcctg tactaacctt gttaagttta
    16501 gacaaatcat tctggcttga tctgccttta aagaaataga ataaattaaa aaggggatgt
    16561 gacactgtca cactcccttt ttaatttatt ctatttcttt atatttattt tgtattccgt
    16621 tacaaactcg cgtacttggt taggttccag agtgacatct ccaaaacttt caagtcgctc
    16681 cgctcctggc agttcttgag tctcaaatgt gataccgcca tgatttacta atttattccc
    16741 gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa atcacgattc ctgaagcatc
    16801 tgtttttact tggatcgata ctttttcatc tgggcttgtc aattccgcag catttagtaa
    16861 accagattgt tttttcaaca aaaatggatg atcgatgccg cccactatca ttgattggcg
    16921 aaaatcactg gcaaagacct cacctaattt tttaggacgt tggaagtcaa atgctgtgcc
    16981 acttactgaa actacttctc ctgtaggcgt tgtatcttta ttgagcgtag caaataatcg
    17041 gctattcaac caaagtatat gttcgtcgat tgactgtgct ggatcaccgg ttaaattgaa
    17101 atagacatga tttgtaggat tgaatactgt tttgcggtca ctgatccctt ggaccgtcac
    17161 tcgccatgta tcatcgttcg tcagcgtata ccgtacttct acatctaaag ttccaggaaa
    17221 tccgttttcc atatctgggc tagtctgata gaaaatgacc gatgcttcgt tttcaccatt
    17281 gatgactgta taatgccact gcttggcttc aaagctaggt gcaccaccat gtaaacaatg
    17341 accagtgtcc gggtctgttt ttacttcgaa cgtttcatca tctaatttga atgtgccatt
    17401 cgtaatccgt ccagccatac gtccgattga agcaccgata taagggtctt tttctagata
    17461 ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt tcattatcga ttattgtttg
    17521 aaaatgaaca acccgtgccc caagatcact gacagacaaa gatgtcccat tttgattcgt
    17581 gatcgtaatc aagtgatagt tttcgccaaa attttgttca ttgatttcca tgttccttgc
    17641 accctctttt ctttcatttg cactgattcc agtatacatg gaaaccgttt tcttaacagc
    17701 ttattccgtg agtaacgaga aaaaatttac tatattctaa taaaagaccg tgaaaaaaag
    17761 gttgtgacaa aaatattgtc acaacctctt tttccgaaca aacggtgttc aaaagcttgc
    17821 atctgcggta ataagcccaa ccaaacgaaa aatatgagga gctatttttg cttggctgtt
    17881 cttattactt gatgcaggac agctttttca caatctcttt ttcaatacga ttccagtata
    17941 taaccatgtc gtcagtacat agtagatgaa atacaatacg aagaatatcg taaacggaac
    18001 ccaaatatct ttgtatggat tttgcatgaa tagaccaaac atttgtaatc cgaataagac
    18061 atggactaca cctaagattc ctggtgctaa gaataacacg cctacttctc tacggatcga
    18121 ttgtttaagc aacccgcgtc gtgttcctat cttttcaagc atttcataac gagcaatgtc
    18181 actcttgctt cctgacaaga tcttgaacat caaacagcta gcaagcatgg tcaagaaagc
    18241 taaacctagg aagaatccca taaattcaaa accagagaat agaccattat acacttcata
    18301 catttcatat ttctggttgc tgtactctac tttttgcaaa gatggattgt tcattttatt
    18361 ttgagcaaca agtgtttcga ttggtttcag gtttgcttgg aagtctttta cctgaactag
    18421 ctgtaaagtg gattgtggca agttcagttg atcaaattct gattgactga ctaccttgat
    18481 ttctttttct tgctgttccg gcaattcata gcttcgaagt tcctcttgta cagtcagttc
    18541 attcattttt ttagcaggaa ctttttcttt cgtgatcttc ggcggtgttc catcttcatg
    18601 ttttatcatg atcaatggtt gagccgcaaa ttcacttgca ttatagtaaa tcgttcccgc
    18661 atcttctttt tgagtataag aagcagaaag tgttggtgaa agttctttga ttttttgctg
    18721 atcttctgcc cgaggattat tcatcactag atcatatcca gaattgtttt ctgtcaagtc
    18781 tgcaatttca tttttaaaac ctaatcctac cgtcaaagcg cccagtgcca aggcaaacag
    18841 catagcgacc attgagagca tttgtgtata ttcacgaata cgaaagttta actgtgacaa
    18901 tgtgaaattg ttcaattttt tcaatgaaat ctgttctgat ttctttagta atgataaaaa
    18961 gaaaatgatc acagaatgga aaatgaaata agttcctaat acgatcgtaa ctaaggctac
    19021 ccctatacca aagacttgat aggtccccac gtgtttcatc atatagtaac cgcagcctag
    19081 aaaagcaatt cccagtacta cttcaaggaa aaacaagaat ttatgctgct tgattcttgt
    19141 tggtgtctga tccgcacgaa tcaatgctaa aatcggtttt ttgacaatcg aaaaagcatt
    19201 gacaacagca gctaataaaa acaaaacagc aaaaaacaaa acagtgatca acaacccgtg
    19261 aatactaaaa ggtgtaaaat gcgaaatatt gagatctaac tgatccacta ataatcggtg
    19321 gacgatactt gtcaatccta caccgacagc tgaacccact aaagtagctg cgatccctac
    19381 taagaatgtt tcagaaaaaa tcatctgtgc gatttttctg ccttttgctc ccagcatcat
    19441 aaacattgca tagtcttttt gccgcatcgt catcaaaaag gaatttgcat ataaaatata
    19501 aacgaatgtg atgatcccta gtaaaacaga accgaatcgg aaaatgataa ctgtagccgc
    19561 aacgatcgta ttacttttca aaaaagcttc gttgcttgcc attgattcaa acatgtaaaa
    19621 aatagcagat gccataacta atcctgagaa taacaccaag tagtctctta aacggctctt
    19681 gatcccagta agtgataatt tccaaagcat agtcaatctc cccctactgt tccaagtttc
    19741 caagaataga tagtatttct cgatagaagg aatcacggct ttgatctcca cgtttcactt
    19801 cttgatggat accgccatcc ttgatgaata aaatacgttg gcaataactt gcagaaaacg
    19861 gatcatgcgt cacaagaaga atcgatacat gatcttttgt attcaattcg tccatcgtat
    19921 ctaaaagatc ccgggcactt ttggaatcca gcgcacctgt aggctcatcc cctaatagaa
    19981 tcgttggttg tgtgatcaat gcacgtgcgg cggcgacccg ttgtttttgt ccaccggaaa
    20041 tctcagaagg atagctttcc aaaatatgag agatggataa acgttctgca attttttgga
    20101 cacgttgctt gatttctttt ggtctgacgc cttgtaaaga aagaggaaca gcgatatttt
    20161 cttgtgctgt caagctttct aataaattga aatcttggaa aataaagcca atctcattcg
    20221 cacgaaaatc tgctagttga tttcctttta atgtggtcac atctttttga ttgattcgga
    20281 tatgcccatc tgttggttta tctaatgtgg ataaaatatt caaaagtgtc gacttaccag
    20341 aaccagaagc gcccatgatc ccaataaatt cgcctttttc tacatcgaag ctgatgccgc
    20401 ttaatgcttg cgtctttttc tcattgtttt ttccgtatac ttttgttaca ttctgtactt
    20461 cgactatttt gttcatttct ttccctaccc tttaattagt ttttccagtg ctgttttcgg
    20521 cacgtctttt ttttcgactt cttcatacga agtgacacct ttatttttgt tccaggtgat
    20581 ttttaaatat gctcctttac gaagcggtcg agtcaattgt cccttgaagt ccagttcttt
    20641 ttccttaccc tctttcgtat agccaggcag gatatatcta tagttgactg tttcttgtcc
    20701 agagtctgat tttgaaactg tttttgtacc atctgtcgta atttgtatat agtattcttc
    20761 tccacccata actacgtaat ctgcgatttt cacccctgca agtaccacaa cgatgaaaac
    20821 aagtagaccg atcagaagtt ttttcacttt ttccccttct ctcttacttt atatactgat
    20881 tatagaaaaa agcgaacaat tctgccttcg aatcaactat cattcttctt tcaattttgt
    20941 cattttcttt aaaacaacta aaaaaagagt tcaagacaat aatccgtcct aaactccttc
    21001 ttcctatctt acaaaaagta tgtcacttac ggtctttcat gtcgcctgta ttgataacag
    21061 gttgcgttcc tttgtctgta atgattgaga caagtaaatt attgatcaat tggactttcc
    21121 gatcatcggt gaaattgatt tcttgtccat cttcgatttg ctctaaggcc atttgagtga
    21181 ttgtgacagc tccttcaaca atggtttgtc gagcagacaa aatggctttt gcttgttgac
    21241 gttggagcat ggcactagcg atttctgttg cataggctaa atggttcaag cgtgtttcga
    21301 ttacttcgac acctgctact gctaaacgct cttgcagctc tttttgcaac tcgtcagaca
    21361 ctgccgtcgt gtttcctcga agcgtcaaat cattctcatt gaatgtatca tacgggtatt
    21421 ggctagcgat atgccgaatg gctgtctcac tttggatttc tacaaaatct tgataatacg
    21481 ctacatcgaa taatgccttt gctgtatcaa cgactttaaa gactactact gctgagattt
    21541 cgatggggtt tccatccaaa tcattgactt ttaaaacaga actattgaaa ttccgaactt
    21601 tcaacgaaac agtcattttt tgtgccaaag gtatcgtaag gaaaaagcca ttttctcgaa
    21661 tcgtacctaa atattgtcca aaaaacaaaa ttacttttgc ttgattagga ctgactacag
    21721 ttgccgaact taacaacaat aacgtgatca accaaagaaa aatacttaaa aaaagtgccc
    21781 aaactttcgc ttgccacata cctaaataga agaaaaacaa tccaactaaa gctaatacga
    21841 ccaatccgac aatccctgca taaccattga tataaaatgc tcttttttct ctcatgctac
    21901 ttcctgcgct cctttatcct tcaataaata tctaatgtta cttttttaat ttttttttcg
    21961 aatacgcgcc attacacctt ctagttctaa ctctttgtct tcagtgatgt accaaccgct
    22021 tgcctgtgtc cctccggctg cctgcggtaa ataatccaat tttgttcgaa cccagtgctt
    22081 ttcttctgta aaatagtctt ctaccaagac ctctagatcc atccctgctg aaagaggcaa
    22141 atcttcccga acagatttaa agataaatcg ttccttcttc ccatcatact tcaatattcc
    22201 tgtatcgata atcggtaaag aataataatc cagctctttc ttcaccgtca ataaatgttt
    22261 gaaaacttca tggtacactt gctctaattg gcgaccttct gaattgttcg ggtaattaac
    22321 catcactttt tctttgtctg gatcatagaa ggtttggctt agttgagtga tataagtatg
    22381 taatctttcc atttctgttt ttaacttttc tctttgacct tcttctacct tcatatactc
    22441 tttcaccctg ttttcttttt agatattttc tttcttattt tactatagtt atgtgcgtta
    22501 agcgatgaaa aacagcaaag attggaagga tctttgctgt tttttcttaa aatatccagt
    22561 tgagtacagg atcaatctgt ttcattgtcc tcttgatccg tcgtaatatt gatcgatgtg
    22621 tctttagcag tgatctttag ctttttctgt ccttgttcta acaaagtatt tgttattggc
    22681 tggtagtctt tttcaatata agattcacca gacgtttgga tagtatagcc actgttttcc
    22741 atctcatgca aagataaatt gccaccgttt aggatgactt ttccttcttt gctgatcaaa
    22801 ctgtcttcta gatggaagtt gctgttttca cctgtgacga tcaagttatc cacattggcc
    22861 tcatctaatc gaagtgagcc taattcatct ttcgcagtta ttgttcctcc tgtaaattca
    22921 ttgaacgata cccagccatc atgtgtctta gtattcactt ttttgattga tacttcgttg
    22981 atatcaattg cactattatt cccttcgatt gtcaattcag ataattcatg atctgcaggg
    23041 acaacaatag tgatttgtgc acttgtgtct tctaaactaa tgattgcacc tgttgatttt
    23101 ttgactgcat tcacagtcaa cacgccattt ttctccttta tttctggctt ttctgtctta
    23161 tcataagtcg tttttatctc aaaatccaat ccgcgtatga ccgaaactgt ttgattttct
    23221 gcatggatga cgattttgtt tgcatccttg attttttttg tctcagtaac catatcgatc
    23281 aaccgaggac cattatccca agtcagactc ttttgagcgc ctaatgtata agttgcacct
    23341 gctaggaggc caccagtaag catcagacca ataccggtaa tcaaaaaatg tttcaatctc
    23401 aatttatgtg tcctccttca aacgcccgct gaatgcagat ctctttttgt aataaaacgt
    23461 cgtcccatcc atttaacaaa gttcatcatg acataaaaaa gcccttttgt tcctttcagc
    23521 aacagtggcg aaataaatac tgcgcttcct gtggcgaaca gaccgacacc gataaagaat
    23581 aatccactca gaatcgattg tccaaacaca ctgattcctg agatgatcat gaataggcca
    23641 acacctaaga tactgataca taaaacatag atactaaaaa tgagcgctag taaagcaacc
    23701 aatagtcccc caataaccaa tataaacccc agtgctagtg gaatgaggac aggagaagca
    23761 aataaaccga taatgattaa ccaaatcagg cgaatttgtc tttttggatt tttcactaac
    23821 tcgttttctg cttgttcttc ttctgaaaag taactgattt ttaattttaa ggcaaactgc
    23881 tttggtgtac catattcatc aatgatctga tctaatgttt ttccagaatc taaaaaatat
    23941 tcctcataat actggatcaa atcaaaacga tcgtcttcag gaatttcttg aagatgatcc
    24001 acaacttcat tcaaatattc attcatttgt gctctcctcc aataaccggt ctaaagaatc
    24061 tcgaaaaaca atccattcat caataatttc ttctaaatga cgacttccca ttggtgtcaa
    24121 cgagtaatat cgacggtttc tgccttcaaa aggttcatca taggtctcaa gatatccttc
    24181 ttttttcaat cttctcaaaa ccggatatag tgtagattca ctgacagaga gactttcttg
    24241 tacgattttt gttaaggcat acccatacat atcttccttg cttaatgctg ctagcacagt
    24301 accatcaaga agaacagttg gaatctggat gtgcattacg tcaccttcta ttctcaagtg
    24361 taatatttta tttcaatcta tattatacaa catataatat agtttcaaaa tcagtttgtc
    24421 aaatacaaaa aaactgacta aaagatttga tcctttttag tcagcaaaaa atggcttctc
    24481 tttgattttt attataacag tggcataatc caatttgtcc acatccagct cagtggcatg
    24541 ataacgatca ttgcaggtat catgtcagct actgggaaca ttttcacgtt gatcattcta
    24601 aaaccggtag cgaccatcaa gaatccgccg caagatttaa aatctaagat catgtctggt
    24661 gtagtcaatg gataaataaa ttttgccaat aagaacaaaa tataaaaaat aatgaattgg
    24721 ggaattgcta tcacagaaac gacataccct aaattacacg caaaaatagc tgctgtgaaa
    24781 aaatcaagga cagatttaga aatcaaaatc gtactgtctc ctgtcatccc agaatctaaa
    24841 cttccataga tccccgtacc acttgcacaa aatagaacaa tgaccgtcac taatgtgctg
    24901 ataaattcat cgtgggtcat accaagcttt tcactaggga agattttaga aataggtact
    24961 tgcatcagcg cagccccctt atttatccca ttacctaaat gaacaatcaa accaatcgct
    25021 gttcctatca ctaaagcaaa aataacagcc ggcatatttt tcatcggcgc aatggaatag
    25081 attcccatcc ccatcgaaca cacaccaaaa accatcgtca gctgtgcttt aaaatgttca
    25141 cttaatttat ttcctactaa tccgccaatt agtccgccaa acaaaacgga acctacattg
    25201 atcaaaactc cagttggcat caaaaaactc ctctcttttt ctacgaagcg aaaaaaatca
    25261 gaaaaatttc tgtaattatt gctttcgctg caagaaagat tgtaacagac agagaagatt
    25321 acaccaattc attttttgtg ttaaagtatt ctttattgga ataagtagga ggaatatgat
    25381 ggatatccat aaattcgaag tttttcttga tttagccgaa acaatgaatt tcaccaaaac
    25441 tgctgatcga caattcacca cccaaggaaa tatttctaaa cagattcttt cgttagaaac
    25501 agaattagac gtcaaattat tcgaacgcgc ccatagaaaa atcgaattga ccgaagctgg
    25561 aagcttattg ctcccatatg taaaaaatgt cgtagaacaa taccatgcta tgcaagaagt
    25621 gcttgaaacc tatacgaaag acaacaatct ttcattaaat atcttaacga taccgacaat
    25681 gatcaattat aaaggttttt ccaaaatcac cgagtttttg aaacgccatc ctgaatttac
    25741 agtccagttg aaagaagtgg aaagtgtcga attaacttac aatgacgcca atgaacatag
    25801 tgacacgatc cactttgcta gaagtttcca ggccccacca agttcgattg agtggttgcc
    25861 tactgaaaca gacgattttg tagcagttct acctaaaaat catcctttag ctagtaaaaa
    25921 gaaattggat ctaagcgaac tgaaaaagga gaactttttg ctccttggcc ccactacgaa
    25981 cttgtatcaa cctgtactag atctgtgtcg cgaagctgga tttgaaccaa aaatctctta
    26041 taaaggagca agaatcgact tgatcatcaa tatgattgca aatgacatgg ggattgctat
    26101 tgttatggaa aaaacagtca agaatctatt aaaagaaaat actgtgattc tccctatttc
    26161 tccaacaaca gagagttatc ttgcatttac tcgtaaagtc ggtgagcatt ctttggcttc
    26221 agatacattt tgggagtatt taaatcaaga agaaaactaa gccctacatt ttctgtccat
    26281 ctatccaccc aaaaaaagag agcgacgcat gactttcgct ctctttcttg catcatgtcc
    26341 ttgtttcaat gacacctcta acatttctcg tagttcaata agaaagcagc gatttttatg
    26401 ccaaaagaac cgtaagaaca tttccctaat cacaaaaaaa cgactaaaaa cccctaaaaa
    26461 ccttcaatag aatttttagg ggttttattc tttttcctat tgtttactta accgtatcat
    26521 tgctgtcgtt cctactcctt cgatactgct ataatcaata cgtccatcat gcagttctag
    26581 aattttttta accagtgaaa gtcctagccc attcccagaa atttgacgac ttgtatctcc
    26641 ttgataaaat ttttcaaaca ttcgatctct ggtctcttca ttcattccta cacctgaatc
    26701 ggtcacttcc agtacaatct ctgtggctgt ctcaaatagt ttgatatgaa tctgtccatt
    26761 gatttgattg tactttatgg cgttatccat gatattcagc cacacttggt acaaaaactc
    26821 ttcattgcct gtgtaattta ctgcagcaag ttcgatatcc aattctagct gttctttctc
    26881 ccatttaggc tgaaaaaaaa cgattacttc tctgatttgt tcatctaatc gatactcttt
    26941 cttttccagt tggatacgct ggttttccag tttgttcaat tttaagatgt tctctgttaa
    27001 ttgtgaaagc tgcgtgatac tttcgataat ccgatataaa aatatttggc gttcttcgtc
    27061 agagagattt ggggcttgta acaattgaac atatccttgg attgttgcta gtggtgtttt
    27121 gaattcatgg gatacactgg acacaaagtc attcctcagt gtctcgatac tattgagttc
    27181 ttggaccata acgttgaaat ctttatacag ttgctgtact tctgctactt tctgctgttc
    27241 atccattcga atcgaaaaat ctcccgttgc taccagactc atatttgtcc ctagcgcact
    27301 gattggatgc aggatacgcc gcccaacaaa aatcgatatt cctgtaccca cgaccatact
    27361 gaaccccgca aagacgaata acgggtataa cgttggtttt ccagccgatt cagcaggcga
    27421 gatagaatgt cgagacaata agaaaataaa accgagaaaa cacagcagtg tcactaagat
    27481 cgaaatgaag acaatactag caaaatagat ccataattgt gaaaatagct tttttttcat
    27541 ctttaaataa ccgccttata gcctagtccc cgtactgtga tgatttgaaa atcttccgtc
    27601 ttttcaaaac gggtccttaa acgtttgata tgtacatcga tcgttcgttc ttctgtattc
    27661 gtatctaatc cccaaatatc atccatcaac tgttggcgag tgaagatctt gttgggataa
    27721 gacagtaatt tatacagaag cagaaattct ttttgaggca gaatcaacga atgctgatct
    27781 tgtaacactg tataagcatc tagctctaat tttgtttgtc cgattatcaa gctactgctg
    27841 taattgatct ttgaacgtcg aagcaatgct tccacacgta ggatcatctc attgacatcg
    27901 atgggttttg tcatataatc atctgctcct aatttgaacc cgcgtttctt atcctcaaaa
    27961 tcggcctttg ccgtaatcat tagaacaggt gtgtcaaatc tggtgctacg taatgagtct
    28021 agcaagtcat agccatccat tttcggcatc atgatatctg taatgattaa ttctatacgt
    28081 gtcgcttcta gttttttcag cgcatcctct ccattcaatg cggtgatgac tgaaaagccc
    28141 gctcgttcca acacagaacg gtaaagcaac tgtaattgcg gatcatcttc tactactaaa
    28201 atacgattca ttgatctccc tctgtttttt gaaaaatttc tagatttttt cttgtagttc
    28261 atcttcagtt aacatttatt tcgtatacta cgatcattgt tctgatatac ttattagtat
    28321 accgaatatt tcaatacggg agaagggtga ttatgcttca attaaaagat ataaaaaaat
    28381 attataaagt cggcgaaacc accaccaaag cattggatgg tgtgtctgtc gctttcagac
    28441 aaaaagaatt cgtagcgatt ttaggtccta gtggttctgg taaaacgact atgctgaatg
    28501 tgattggtgg actagataat tatgattccg gtgatatggt aatcaacgga aagtctacta
    28561 aagactttaa ggacagtgac tgggatgcct accgaaacaa ctctatcggt ttcatttttc
    28621 aaagttacaa cttgattggt catctaggca ttattgaaaa cgttgaactt gggatgacat
    28681 taagtggtgt ttctaaagac gaaaaacgaa aacgggcaga agatgcattg caccgagtcg
    28741 gcttgacaaa tcatatgcat aaaaagccaa atcagttatc tggtggacaa atgcagcgag
    28801 tggccatcgc tcgagcacta gccaatgacc cagatatcct tttgtgcgat gaaccaaccg
    28861 gagcgttaga tacagaaaca agtatccaga tcatggaatt gatccaagag ttatcgaaag
    28921 aaaaattagt gatcatggtc acacataatc ctgaactggc ccatcaatac gcagatcgaa
    28981 tcattgaatt ttctgatggt aagatcttaa cagattccca tcctcacatc gaacgaccaa
    29041 aagatgatca atttaactta cgcagaacaa agatgagttt ttggacagcg ttaaagcttt
    29101 ccttcaacaa tatccgaaca aaaaaaggac gtactttctt gacctccttt gcttccagta
    29161 ttgggatcat cggaatcgcg atcgttctgt ccctttcttc tggattccaa aaacaaatcg
    29221 ataacacgca agctgaaacg atggcgaaat ttccgattac tatttctaag gtaacgacta
    29281 accagactcg tgacgatgcc ggattgggtg caagcaaagc ggattatcca gacagcaaaa
    29341 cgattactgc aaaagtcagc gaagaagata aagcacagca cacgaataag attgatcaaa
    29401 catacgtcga ttacgtaaca gacatcgatc ctaacttaag taataatatc ggatttaccc
    29461 gtactacagg aatcaatctt ttacgtgatg taaatggcaa agtacagcct gtcagcttct
    29521 cgaatcaaaa tcctgatacg gaatctcttt ctttatctag tacgatgtca gcaatgacag
    29581 gtgtgggagt gtcaagtttc cctacacagc ttgacactag caaagaaaac tttttaaaag
    29641 acaattattc tttacttgcc ggaagttatc cagcctcagc aacagatgtc gtgctgatcg
    29701 tagacggcaa taataatact aatatcaacg cattgaagaa tcttggattt gatgtgaaag
    29761 aagacgaaaa acttgatttt gatgatatcg ttggtacaac attcaaatta gtaaacaaca
    29821 atacttacta tacaaaactt ccaactggga attttatccc taatacagat tatgatgcga
    29881 tgtatcagaa tgcttctgat gaattgaaga tctcggggat cctacgcgtg aaatcttctt
    29941 ctacaatgaa tctcttatct ccaggaatcg cttatagcga tcaattaacg actcaaatcg
    30001 tcaacgaaaa caaagaatca gaaatcgtca aagctcaaag agacagtggt gtaaatgtct
    30061 taactactga aaaagttgac gaaaatgcca aacaaacatt gcttagttat ttaggtggag
    30121 acagcttgcc ttcaagtatc atgatttatc cgaacaattt tgaagacaaa gaaaagatct
    30181 tggattatct agatgattac aataaaggca aatcagatga agataaaatc atttatactg
    30241 atttagctgg aacgatgact gaactgactg gcggattgat ggatgctatc acttatgtcc
    30301 ttattgcttt tgcagggatc tctttagtaa caagtatgat catgatcagt atcattactt
    30361 atacatctgt gattgaacgg acaaaagaaa tcggtgtact aaaagcttta ggtgcacgta
    30421 aaaaagacat cactcgcgta tttgacgcag aaacgtgtat tttaggtatt tcttctggga
    30481 tattaggtgt gtttattgca tggctagcta cgttccctat caatagcatc ttatacaaca
    30541 tgacggatct aaaaaatgtt gctcaactaa atcctgtcca tgcgattatt ctagtgatcg
    30601 tctctactat attgacgatg cttggcggtc atcttcctgc acgaatggca gcgaaaaaag
    30661 acgccgctat cgctttgcgt gcagaataag gttgtgacaa ccacatttag atttgagtca
    30721 taagagcgct gaaatagtag aaaacagcaa gaaacgagga agtaatatgt tccttgtttc
    30781 gcgctgtttt ttttaattat tttttataat tctggtatac ttttctttcc tttgtaccac
    30841 aactcttctg gcgtatacct gtcttttcca tttatgaata ctgttccatc tgcttcaatc
    30901 gttacttcag tcccaatcca catattacga gaaagtgcga gaagattttt tctttttcct
    30961 cttccttttt ttccaaattg caaggcatcg gttatctctt ctgtccaatg agatttcacg
    31021 tattttactt ctccgttttc aaatgtaact ttcaaaatat tcgctatatt ttccactttt
    31081 actgctttag gaaatgactc ctccattttt ttcactttcc ttcctccaac actaagtatg
    31141 cgctttcttt tagtgataag tatatcatag tttattctat tatcaaaaaa acgcatccat
    31201 tccattgtag aatactttac cgaacaagtt gaattgtttt cctctttttc accttttact
    31261 atagataggt aaagacaact aaggaggaat ctctcatgac agatcaaccg tatgatttaa
    31321 gaaaagtcat cgaagaacta aaaacacttc caggtcaata tcacgaaaca gatattgaaa
    31381 tcgatcctga agcagaatta tctggtgttt accgttatat cggtgctggc ggtacagtaa
    31441 aacgtccgac tcaagaagga ccagctatga ctttcaacaa tatcaaaggt ttccccggca
    31501 cacgtgtgaa catcggaaca atggctagtc gtaaacgtgt aggtgaaatt ttgcaccatg
    31561 attatcgaac acttggtcgt ttcttgaaag acgctgtaga aaatccaatc aaacctgtga
    31621 aagtagacaa agcagacgct ccagcacaag aagtcgttca tctagctacc gatgcagact
    31681 tcgatattcg caacattttg cctgcaccta caaatactcc tgaagatgct ggtccataca
    31741 tcacaatggg tgtagtctta ggatcagatc cagaaaaaac aatgagcgat gtaacgatcc
    31801 atcgtatggt tctagaagat aaagatacga tcggtatgta catcatgcct ggcggacgcc
    31861 acattggtca cttccaaaaa caattcgaag ccttgaataa accaatgcct atcactatca
    31921 atattggttt agatccagca attgccattg gcacgacttt cgaaccacct accactccac
    31981 ttggctacaa tgaactatgg tgtgcaggag ctttgcgtaa cgaacctatt caaatcgttg
    32041 atggtatagc agtcgatgaa gtgggtattg ctcgtgctga attcatcatt gaagcagaaa
    32101 tcatgccaaa tgaaacaatg caagaagata tcaacacgaa ctcaggaaaa gcaatgccag
    32161 aatttcctgg ctatactggg gatgctaatc cagctgtaca agtagtgaaa gtcaaagcag
    32221 ttactcaccg aaaagacaat ccaatcatgc aaacaacgat tggtcccagt gaagaacacg
    32281 tatctatggc cggtattcct acagaagcaa gtattcttgc cttggtcgat aaagctattc
    32341 caggaaaagt agtaaatgtc tacaatcctc ctgcaggtgg tggtaaattg atgaccatca
    32401 tgcaaatccg taaagaaaac gaagcggatg aaggtattca gcgtcaagca gctattcttg
    32461 ctttatcagc cttcaaagaa ttgaagacag tcattttagt cgatgaagat gttgatattt
    32521 tcgacatgaa cgatgtagtt tggactttca acactcgttt ccaaggagat aaagatatca
    32581 tcgtgatccc aggaatgagg aaccatccat tggacccatc agaattacca gaatataatc
    32641 ctggctctat tcgtttccgt gggataagtg caaaaactat ctttgacggt acagttcctt
    32701 tcgatatgaa agaacatttt gtacgtgcaa aattccaaga agtagaagat tgggaaaaat
    32761 atttgaaata atgaatgatc tgcaactgtt ttgacatcat aaaaaggagt gcggaaaatg
    32821 gaacaattgg cacaacggaa gaaaaaaatc gtgattggtg taagtggcgc ttcaggcacc
    32881 atttatgcca tcaacctagt gaagaaacta aaagaatatc cgatgataga aacacacgga
    32941 atcatcagtc catgggcaaa acagaactta aagctggaat ccgacttatc actaactgaa
    33001 ttcgaaagcc tgtttgatta ccattattca aacaaagatt taggtgctgc tgtcgccagc
    33061 ggatcatttt tgaccgatgg aatgatcatc gtaccggcaa gtatgaaaac agtcgcaggt
    33121 atctctgttg gtttaggcga caacctgatt gcacgtgctg ctgatgtaac aatgaaggag
    33181 caacgaaagt tagtcattgt tcctcgtgaa acgccattaa atgccattca cttggaaaac
    33241 ttgactaaat tagcacgact aggtgtgcaa atcataccac caattcctgc tttttataat
    33301 cagcctcaaa cgatccaaga tcttgttaat caccatacaa tgaaactttt ggatcaattt
    33361 gaaattgtaa atgagcattc aaaacgttgg cagggtgatt aatatggaaa aagaaacgat
    33421 agttgaagta accaaactag gtttcacgat ccaagctgct gttaaagtaa tcggtaaaga
    33481 cttattgatc actttaacag gcggagatac accacatatc ggaacagtca ctacattttc
    33541 tcgtgataca gagatgcaaa ctgtacgttt ccctagccac gatggtcggt tccataaaga
    33601 cgatgtctta gccgaaaaaa tagctgtgat catcaaaact gccgttccgg gaagttgtac
    33661 gatcacttcc ggtatccatg ttgaccatat ctccaatgca caaatcaacg cctcttttcc
    33721 gatgtcagaa gagttaggtt acaagatttt agattggttg gaaaaaatag atttccatat
    33781 caaagaacca gtttattata aagatggtga aaaaccatta tgaaagaaga ggttgtgaaa
    33841 tcagcgttat gacctgagta tgagagaaaa aaatggaaaa atagcttctc atattttgcc
    33901 gtttttgagc taataccgag ggacagctga ttgaacgccg tttatcaaga ggttgtgata
    33961 aagccgcttc gtttcgagta gtaagaaaaa ttttgtaaac atgggtttcc acatttttgc
    34021 ataaatttga cttactaccg agaaactggc ttttgaacac cgtttatcaa gaggttgtga
    34081 cacgtctttt gtcacaacct cttttatttt tgcttcactg taactgctac aatatcaccg
    34141 atttcttttc cgatgttttt acgaatatct tttcgtagtc caaggatgta gcagactgaa
    34201 ccatctggat ttttcacgcc catattcacg atactgcctt catacggcac attgtcaaaa
    34261 gttgcttgga ctttaactct tccagcaccg aattcttttc ttatatcata cggaaaaata
    34321 atatatgccc cgcccttgcc gacttcggaa gcatagattt ttgattcaaa agaataaatt
    34381 tccctcattt tatttctcct attttttaca tctagtatag catagcaaaa aaaagaagga
    34441 actgtgacaa aagtcttgtc gcagttcctt ttttcgaata aacggtgttc tcctgccaac
    34501 tcctcggtca caagtcacaa tagtcaacaa acatgagaag ctgttttttg aaattgctcc
    34561 ttgtgactca gagttaaacg gcaggctcac aacctcttcc ttaaacggtg ttcaaaagct
    34621 tgcatctacg gcactaagcc caatcagatg aaaaatatgg agtgctattt ttatctgatt
    34681 gttcttactg cttgatgcag agcagctttt tcacaacctc ctacatggtg ttcaaaatat
    34741 tattcgcctt ttgcttcttt cactaatttt gcgaatgctt gttgttggaa ttcagcagct
    34801 aaggctttcg tacgttgttc ttgctctgct ttgctcattt ttggattttc agtcattgct
    34861 tttaccatac cgtcttgaac ataagctttt gcttcacttt ccatcgtact ttgctgtttc
    34921 ttcactaatt cagctaactt ggctgcttgg tccgtactta attctaccca tgtctctggg
    34981 attttgaatg tattcactcg gctgtcatat tcgtgattgt tatcagcaat attgaaccag
    35041 aatttataca tgtcgttttt gtattcccaa taagtagttt tcgtcacttt ttcagcatcg
    35101 ccgtctgtct tttctacttt atttgtttcg ttatccgtac cagttgcttt tggtttttct
    35161 tgtttttcat ttgttcggta aagatacact ttttcttttc cattccctaa agctttatac
    35221 aaaagcatat ctgctccttg ggaatcagca ctggaaacaa ttttttctgt ttttgtttcc
    35281 gttacttttt ccattccaaa gtgattggac aaattagcga cgatcaaact tagtgaagcg
    35341 acgaagatta gtccgaaaat caaagataaa aaggtttgcc atgttttttt agcgaacacg
    35401 ttcgttaaag caaaagcaag caagctgaca atcaaaactc caataatcat gcattatccc
    35461 ccgctttcat tagaacaggt tcttttttct tcaataaaaa ggcaataata aagccaagaa
    35521 tcccaaagat gatcgctacg ataaacgcgg catggtaacc tgacagtgtg gcattgattg
    35581 cctgatcttt ataagctaat ggcgtattat ccaatacaga tttgcttggg atatttgctt
    35641 ttgtcacatt cgtcaacaca ctgatcaaaa tagctgtacc aatcgagctt gcgatttgac
    35701 ggaatgtatt atttaccgct gtcccatgac tgatcaagtt atttggcaat gcattcatac
    35761 cagaagtcgt taccggcatc atgaccatgg agatcccaaa catacggatc gcataaagga
    35821 tcacgatata taaaactgga gtcgtttttg tcaagaaagc aaatggtgca gttgccgctg
    35881 tcaaaatgaa catccctgtg atggctaaac gtttcgcgcc gtatttatcg aaaatcgcac
    35941 ccgtcacagg catcatagcc cccatgatca atgcacccgg caacaacatc aagccagaat
    36001 ggaatgctga ttctccacga atattttgaa tatacaatgg tacgaccatt tctgccccta
    36061 ccatcgccat atttgttaca ccagctaaaa cagctgcaat cgtgaatgta gatgatttga
    36121 atacacgaag ttccaagaat ggatgttcca aatgtaattg acgccatgag aacaaggcga
    36181 tgaaaatcac accaacaact aagaaaccaa ttactttcaa gcttccccag ccgtcatctc
    36241 ctacactaga aaatccgtaa agtaaactac cgaatccaat cgttgataaa atagcggata
    36301 aaatatcaat gctaggattt gacaatggaa taacactttt cattaggaag aatgctaaaa
    36361 ccaagacaag aacaacgatt ggcagtacca taccaaacaa gtcacgccaa tggtagttat
    36421 caatgatata accagataat gtagggccta aagcaggagc aagtccaata acgatccctg
    36481 acatccccat tgctgatcct cttttttctg gcgggaaaat agatagcatg atattttgca
    36541 ataatggcat cgatactccc acaccgcaag cttggatcaa acgaccgatt agtaatgtac
    36601 taaaattagg tgctgtaaag caagtaatcg ttccgatcaa aaagatcgtc atggcagaaa
    36661 gatataaatt tcttgaactg aatttattga tcagccatgc tgttattggg atcatgatcc
    36721 cgttgactag caagaaacca gtcgttaacc attgcacatc agacgctgaa atatcgaaag
    36781 ctttcatcaa tgtaggaaaa gctgtcgtca ataaggtctg attcaaaacg gtacaaaatg
    36841 taccaattaa taagacaaca actaaaagtg tccgattata tggttttccg taaatatcta
    36901 cgggttggtt gttactcata tttctccttc tttctgaatt ttttctgaaa ctactagaat
    36961 actttagtcc taaattcgct atttgtcaac ctacttgaca cataagttaa tacggatata
    37021 attaagggaa gcaagaggac aaaaggagtg acaaaaatgg ttggggcttc ccttaaacgt
    37081 ttgttggaaa atgatcaatt ggttgttggc atcagtgaat taagtgatat cgtaggtgtg
    37141 tctccacgtc aattaaggta ttgggaacaa aagggattca ttcgttcgat cgaagctgat
    37201 gcgaattgtc ctagaaaata tcgtttacct acagtcataa aagtagaact gatcaaaaaa
    37261 tatttagatg acggatttac tttgtcaaaa gctgcagaaa aggcagaaat ccgccaaaag
    37321 aaaatgcacc atgtccgtaa agttttttca aaagcaatca aagatatcga attgatcgac
    37381 gaacgctata cgattttaca aatcggtaac ttcaaagaag agaatgctaa gatgtacatc
    37441 attcatgaca acgagacaga cgaattaact tacaagctcc ttcctgtcga tgaaaaagct
    37501 gactacgaac agttgttaaa agaactctaa acgccactaa tctcctaagt accaagcttt
    37561 ttaataagta gatgctacat aaatacttga aaaaaggcct gtttgaacat aagaatatcc
    37621 gaacggtgat ctgcgtttct ttcatataga aaggaattct ccatcactgt tcggatattt
    37681 ttttattttt ttcggttact ttcttctaat acatctgtta gccaatttgc atattctgtt
    37741 tcccgattga tcgcatgcgc taaaatcagg tagtgtccgt aattttcatg tttttctttt
    37801 tcagtaggaa agatcgtttt ttttcgctct aatagatgaa gtcgtttttc ttcatgaagg
    37861 tcacgctgct gctgaataat ttctactaat gccggatcat tcacgtcttt gacgaaatac
    37921 aatttcagaa caaattcgtc tcgattaacc ggtagttcat tggtgggtgt atgaatccac
    37981 tctgccaatt gttccttccc ttcttctgtt aattcataaa ctttcttttc caatttttca
    38041 cctgtgatct ctacttgatg ctgaatgatt ccttgctctt ccatttttgc aagttcagga
    38101 taaatctgac tatgttttgc ttgccaaaat tctcctattt catttttgaa cgattgattc
    38161 aattcatatc ctgtcatctt cttcttactt aataacccta acaaaatata tcgaagtgta
    38221 ttttttcttg ccatcagttt tccctccttg acaaatcttt aaatccattc tatcataaat
    38281 gagtaatcga aaacatgtag tttattacat gttttaattt ataaggagat gcttaaacat
    38341 gaaagaattt aaagaattgg aagattttat tggaactcac tttatctata cgtatgacaa
    38401 tggttgggaa tatgaatggt atgccaaaaa tgaccatact gttgattacc gtatccatgg
    38461 cggaatggtc aaaggccgtt gggtaaaaga ccaagaagct gatatcgtta aattgacgga
    38521 cggcgtattc aaaatcactt ggacagagcc aacagggaca gacgtggcat tggactttat
    38581 gccaaatgaa aagaaacttc acggaatgat ctttttccct aaatgggttg aagaacatcc
    38641 agaaatcact gttactttcc aaaacgaaca tatcgcagaa atggaagcag ctcgtgaaaa
    38701 atatgaaact tatccaaaat tacttgttcc tgaatttgct acgatcactt atatgggtga
    38761 tgcaggtata gacaatgaag atgttattag cgaagaacca tacgatggaa tgccagatga
    38821 tatccgtagc ggaaaatact tcgatgaaaa ttataaacga gtaaaataag gttgtgaaag
    38881 agaggttgtg aaatcagcgt tctgacctga gtattagaga aagaaacgga aaaatagttc
    38941 ctcatgtttt gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc
    39001 aagaggttgt gaaatcagcg ttatgctttg agtaataaga acgctagtta gaaaatagct
    39061 tttcatattt tctaggctag tgggcttatt accgagaagc agccgtttga acaccgttta
    39121 tcaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaat ggaaaaatag
    39181 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
    39241 atcaaagatt gtaacaactg catattcaca cagttaaatt agtgacttca aaatgaaata
    39301 atgctcccag caaaaaatta ggctgggagc attatttcgt tattctgttc atcaaaaatc
    39361 agcgttttga ttccatttca tcacaagttc ctcctcattt actgcttcat ctttttgctg
    39421 tgtttgaatt gtaaaacctt gtgaaagata gaaatgaata gctgattgat ttttttgata
    39481 gactgtcaaa gtcagtgttt tattggtttc ttttattttt ttcattagtt gcgtaccgat
    39541 tctttgttgc cggtattccc ttttcacaaa gattcctgcg ataaactgat caatcacccc
    39601 agcaaaacca ataatttctt gatctatttc tgctaagtaa atcactgcat ctggtaaagc
    39661 tttcctaaca gattctactt gattctccca ataagttgat ggaatgaatt catgagaatc
    39721 caagttagca tgcagccaaa tcgatagaac agattccagt tctttggcag acaattttgt
    39781 tgttgtgcgg atcatttttt ctctccttac ttattcattg attttaaaat aaaaaccatt
    39841 tttttgaagc tcttttacaa gttttcgccc ctcaaaaaag tatatcttct cttgttttac
    39901 ttttctaata ccaaaagaaa cagcaacttt aggatcatca gctgaaacga ttcgataagt
    39961 tccaccaggc aattgatcca gcttgacttt cccatgatgg tcgctttgta ctgaaaacgg
    40021 cacaccattt ctagtaagtg tttttttctt atgtttcaaa atgaatgaac taccatttac
    40081 cggtttccct gacgaatcac taataataaa tgaaaaatca agcacttttt tacttaatct
    40141 atactgatag attagtcgtc cgataaacac taacagtatg actacagcta ttatcgttcc
    40201 tgcaagaata aaccattgcc gcatggttct ttcctgattt ccttttcctt cttctttttt
    40261 taccgattcg ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg tgttgatcat
    40321 atacggcgta catgtaagca aggtaactaa gtcttctcct tctgaaatcg ttagaacaga
    40381 cgtgtcatct ggcagtacca cttgtatatt tttaacttga taagccaatt tctcccccaa
    40441 aacagtcaaa ataaacgtgt ctccgttttc taaacgatct aattcggtaa ataaccgtcg
    40501 attaggtaaa ccgctgtgag cagagatcac tgaatgcgtc gaatttccac caagcgggaa
    40561 agaggttcct tgcaagacgc ctgctccatt ttcaaggatc ctaccattga gccgatcata
    40621 aagtgaaata tttaattgga tcttaggtat attgatactt ccgatcagcc attcctctaa
    40681 ttggctggat gttgtacttt catttctgtt cttttcattg aaaggatcag taccaggatt
    40741 gaatccttgg tcagttaatc gttggttttt ctcctccatt ttctttcgct gcacttcaat
    40801 ttcagctgcg gtccgttttt gtacttgttc gatacgtttt tgatcaataa aactatttaa
    40861 agaatccaca taaaatggat ataaagcgat cagtaaccct acgacgatac atattaataa
    40921 aaacaaacga aacaaaagtc gacgagactg tttttgctta ctctgcttca catgtgtcct
    40981 cccttgcaag tttttttgtt ctttctttta ttatatgggt tcaggaaaaa aatagaaaat
    41041 gaaagccgat gaggttgtga aaaagctgtc ctgcatcaag taataagtac agcccaaaaa
    41101 aatagctact catatttttt gttgggttgg gcttattacc gcagatgcaa gcttttgaac
    41161 accgtttaag gaagaggttg tgaaaactgc gttatgcttt gagtaataag agcgctagtc
    41221 agaaaatagc ttctcatatt ttctaggcta gtgagcttat taccgagaag cagctgattg
    41281 aacaccgttt accgagaggt catgaaaaag ctactttgtt tcgagtatta agaaaaattc
    41341 tgtttcaaaa caaggagatg caacaaaact tatgtcgcat cctctctcct tagaactttc
    41401 acttatgttt ttcttgtaaa attcataaat attttcatga attttctaat ttcttgccta
    41461 cctctcgcat aggatatatg atgaatttat ctaaagtttg caaaggagtt cgacaattga
    41521 aaaaatatgg cccattttta gcaatttttt tcattgcttt ggcatttctc atgcctcatt
    41581 tattcacaca taaactaatt ttgggtgctg atgccccatt ccattacaat cggttctatg
    41641 atgccgcgca acaaattaaa tatggggatt tccattattt tccttccctt tatggattcc
    41701 agcaaagtgg caggatcgtg aatgccgttt acggcccatt tttcgcttat ttacaaggat
    41761 tgatcgtgtt gcttgcaggt agctggtttc attatcagat tctttcaaat ttcattcttt
    41821 atcttatttc aggattttct atgctgattt tactgagata tgtcaatgtt cgaaattggg
    41881 taagtgtacc agtagccgtt ttattcatga gtacgtattc tattcaatac tggtctatca
    41941 accaaggatt cactagctgg gggacttgtt tttatccttt atgtatgatc cctattgctg
    42001 atttcgtcct tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg ggacttatga
    42061 cacagatcca tttactgtct tcgatcatgt tagctttgat gtatatccct ttttatcttt
    42121 attacttttt taaccagact gaaaaaggaa aagcactgct tcaactgatt aagtcggtct
    42181 tgctttattt tgttctgaca gcaaatattt gggcaggatt agctattgtt tacagtggga
    42241 ataagattct tcctcccttt gttaacagag aaatgagtca aaaggcgata gatctggatg
    42301 gcagttattg gctccattac cctaaggcct ttcctatatt gatattatca ggttttttgc
    42361 tttttatttt ctttcaacaa aagaacacct tattccaaaa aatcgtgttg tacacaagtc
    42421 ttgttttctt ttttctttct actagcttga ttccatggac gacattgatc gaaaaaaatg
    42481 tgccttttat tagtttgatc cagtttccat ttcgattttt cgtgccattt acgctattgc
    42541 ttctacttta ccttgccctt tcactgaacc agtggtctga acaaaagtgg ttccgtcttt
    42601 taagtatatt tggcattctt attttttcag cacagacgat ccagaactta taccagcatc
    42661 tggaaaagtg ggaaaatgaa acatttgtca gtcgacatac ttatctattc gatacgccgg
    42721 aagaagcaag gaaaacattc ttcagcaaag atatgtctac cagcttgttc gttttccaaa
    42781 aaaccacacc tgactatctg ccaatctata aggagacaaa agacaataaa tacgaccggt
    42841 ataacgaaga aatactagaa aacgaaacaa cttttataaa aacacatcca ggcggtcttt
    42901 tgacaatcaa gtggaaaaac actggggaca aaactgttca tatccctgtt atcgtctacg
    42961 atcggactgt gctacagcaa aatggaaaaa cattgactga ttatgaagta acagatatcg
    43021 ggaccccaat cgtgaagcag caaaaaggaa tcaacgagct cacgcttcat tatcaaacgc
    43081 caatctattt ctatttcatt ctttctctta ctttgattgg ctggtttacc ttgctatgtt
    43141 tatttattta tcatagatac aagcttttac gtgcttaggc aaaagggata aatacatccg
    43201 atcaaaaaac tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt
    43261 atgttatatg ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat
    43321 cactgatgat tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc
    43381 cttcagggac gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta
    43441 ttttcttttc cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa
    43501 gaagaaggca actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat
    43561 cacctaggaa aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact
    43621 caaaataaaa gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa
    43681 tgtcctgatc atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt
    43741 tcttttgaaa ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg
    43801 catttccttc ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact
    43861 cttttccgtg ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag
    43921 gtacttgatt ctttaccgga cagaacttca ttagcttcgc aggttctact tcttcagatt
    43981 ccagtaattg gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat
    44041 ctgctaattc ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg
    44101 tcatgaattt cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa
    44161 tttttgtcac aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc
    44221 aatggaccag gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt
    44281 tcacgacctc tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa
    44341 acgaaaaata ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct
    44401 ttttcacaac ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac
    44461 ctaattgctt gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg
    44521 gatctttttc ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc
    44581 ctacttcttg gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat
    44641 cgattcccat atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt
    44701 tgctagggaa aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg
    44761 gaataacagc aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag
    44821 ataaaggaat cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt
    44881 gcttactacc tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc
    44941 ctgattgcag cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg
    45001 cttggactcc attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt
    45061 cagtccctac tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga
    45121 catctttgat attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg
    45181 ctgttcctgt tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt
    45241 tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa
    45301 caacaccaaa agcgatcaca cacaacacga agttgataag atctccacct agacccgctt
    45361 tgatagctaa cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg
    45421 gcaagatatc tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg
    45481 gcacagcagc aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag
    45541 ccaacgctgc tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac
    45601 gatacatcgc aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt
    45661 tgaaacgagc cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac
    45721 ctttaagatt cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt
    45781 gtgtccccgc ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga
    45841 taggaatcgt cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa
    45901 ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat
    45961 tattgattcc agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg
    46021 accataagat aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg
    46081 gcaattttct ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga
    46141 tccctacgaa tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt
    46201 taaccatgat cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt
    46261 cacttgtgac accaaagata gccccagtga tccgattgat taaaataaat gagatcccag
    46321 ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata
    46381 aaatatgaag attaccaata attgcccaac caatattttc aattactccg cccgttgtga
    46441 caagcggcgt ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc
    46501 cagctgccgg cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact
    46561 caaaactaaa tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg
    46621 tattatagcg tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc
    46681 attctataaa acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt
    46741 ttttaagctt ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg
    46801 tttattatga acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac
    46861 tatttgctat tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac
    46921 tgcaagaatc tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag
    46981 aaacttactc aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata
    47041 agacacgtgt cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg
    47101 ccttgaactt tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt
    47161 tggaaccaga agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat
    47221 tcacaatcac tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg
    47281 tcaaaaaaga atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag
    47341 tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac
    47401 atttttggaa tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa
    47461 ctattccaaa caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg
    47521 ttaatggaaa atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct
    47581 tgtctgcaga attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta
    47641 ctagtagaga tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc
    47701 agacaaacaa ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga
    47761 agcttgccga tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt
    47821 tgttccaact attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag
    47881 gattcacagg agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc
    47941 ctctttatct tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata
    48001 atcaactgcc gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc
    48061 ctatggtcac cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga
    48121 ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata
    48181 cggaatattt gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag
    48241 accgtgtcca tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa
    48301 acgaatatga gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct
    48361 tgcgttacac gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg
    48421 aagaagaact agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag
    48481 aattaggaat ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg
    48541 atattcccac aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat
    48601 cttgcttcat caagcaggca gatgtactac aggggattta tttcttcaat gatactttta
    48661 caaaagaaga aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt
    48721 ccctttcgcc aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg
    48781 tagaaatgta ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag
    48841 acggcttgca catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc
    48901 aaatgaaaac tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag
    48961 cttattcttt ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac
    49021 aagttacttt cgagttatta gaaggttctc ctctagaaat ttacgtctat gaacagtctt
    49081 attctttagc ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg
    49141 gtattgtttg atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg
    49201 cgaaagcttg gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa
    49261 ggcgtcagcc gagaagactc actctctttg cttttagctt atggcggaaa agaacactct
    49321 ttttcaaagg aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg
    49381 atccagacga tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta
    49441 aaagaagaaa aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt
    49501 gaaaaaatgg gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac
    49561 ggaaaacctg cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa
    49621 gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct
    49681 caaccaattg gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat
    49741 acgacttttc ttacgatcga ttacttgaaa gaaaagtggc atgaccatgg ataatccaga
    49801 gatctccgtc gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta
    49861 cttagaagtc gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa
    49921 agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga
    49981 caaaagcttt tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg
    50041 gggtgagcat agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg
    50101 ctggtctttc caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt
    50161 cttttactta aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta
    50221 ccgtttaaca aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat
    50281 ctgcaatccg accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta
    50341 tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc
    50401 tactggaaat tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact
    50461 gcaagagatc cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc
    50521 taggctttcc aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat
    50581 agcaacttct aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc
    50641 tgtcaatgga aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc
    50701 tgatatcgtt catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat
    50761 cagtcagacg atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc
    50821 tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt
    50881 catcaacgat catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga
    50941 tgaactaggt tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc
    51001 catcggcgta atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct
    51061 tgagatcatg gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc
    51121 tactgctaaa aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca
    51181 agtagatggt tttattctag tgtattcaga tagtcaagat actgtcattg attatttata
    51241 ccaaaaccac atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt
    51301 ttatgtagat aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa
    51361 tggacataag aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg
    51421 atatttcggt tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt
    51481 actagagcat ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac
    51541 agcattggtg gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta
    51601 tggctatgtc attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac
    51661 attaacacac ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc
    51721 tatgcaaaag ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat
    51781 ccctcatcgc ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc
    51841 tgactcacta aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata
    51901 ctacttctaa cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt
    51961 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    52021 gctgaaattc acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt
    52081 taaacgcttc tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc
    52141 aataaaaaac cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa ggcgaatggc
    52201 aagtaatgca acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac
    52261 ggtacgagag aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt
    52321 gataagccaa cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga
    52381 tactgttctc caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa
    52441 cgagaaggtc ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat
    52501 ttgatcagta acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata
    52561 ctcatgatcc cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca
    52621 taaccattca tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa
    52681 gaaagaacga gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg
    52741 attgcccccc caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc
    52801 gctttcatgt tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt
    52861 cctaatatcg tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata
    52921 ccagcgatat gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct
    52981 gataaaggcg agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt
    53041 gcatcattga atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc
    53101 gttgtaaatg aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt
    53161 aaaaagaaac gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca
    53221 gataaaatcc atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc
    53281 cctggagtga ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat
    53341 ctgccataaa acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg
    53401 aatccgatga tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct
    53461 tttaaagaaa tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat
    53521 gaaaaaacta ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa
    53581 gacttatttt tttatttttc ttctaaactt ttttctacct caatccccca agaatctttc
    53641 ttgaagtgat tgatgatcaa tggaataacg aacatgacca ctgtgatagc tgctactaat
    53701 aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata cgacaacgat gatagagaag
    53761 atcagcatgc agctgactac ccaagctaat ccgtttccgt ttttaccaat tcggtaagga
    53821 cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg aaacagcaat caaaatgtac
    53881 acgatacaat aaataacagt tgtagcattt gtaagtgtca agaatacagc acttacgtca
    53941 tccattacac cataaagcaa agcaaataat gaaataacga ttgattgtgt tagaacgaca
    54001 tttctagata cgccgtattt gttttcttta tggaatccga attttggtgg aaggaatcct
    54061 tcacgagcta cttggatgat tgtttttgat ggtccagtaa cccaagctga taattgtaaa
    54121 agcacaccaa taaataccat gaagctgaag atatttccga tcacttctgg taagcctagg
    54181 atttgacagt caatcaagat aggctgcgtg atatttgcta attccatttt tccatctggt
    54241 acgacatttg ctacaagcat cgcattgatg acattcaata aaactaatcc gatcaaagcg
    54301 ataaatacgc cttttgtata attttttgtt gcgtctttca aacgaggcat gtaaacagaa
    54361 ctcatttcga tcccaacaaa aataaatgtg ataccagcta agtatttcaa tgaatcaata
    54421 tgttccaagt cagggaaagc ttttgaccat gaaaagtcac ctagatagcc gtttgagact
    54481 agtccgactt tgaaggctgc caaaacacct aaaacgaaca tgatgactac tgggatatat
    54541 acccctagcc atacaccgat gttcccgccg acttttgcca tatcgaattt caagttcaag
    54601 atcgtgatga tccagtagat aactaaaata caacctaaaa cgaaccagtg attattgcca
    54661 agttctacat taccaaatgt gtttcctaac aatggtccta aagtagaagc gaccattacc
    54721 atacctggga acatttgcac ccataatagc catgcaacga caaatcccca tttgctgccc
    54781 agtgctgttt ttacccataa ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca
    54841 ccggaaacca ttgatatcgg accggcaaaa aacaaaactg caaaaattga ataaaaaatc
    54901 aatgtccaac cgactgcggc taacgtagga atactccgaa ccgttgcaca cagtgccatt
    54961 gtcagaccga taaaggtccc aagacccatt ttacttactt cagatttact caccgttgac
    55021 actctccttg attttttgga taaaataacg gtgtctttga agtagtttca gcaataaacg
    55081 aaacaattca gcgaatattt gaggggattc tttgagttta ttgcttgaag ggtttcttca
    55141 aagacaacct caaaacaaag ggatcgaaga gcttaggtct ccccttgctc ttcagatcca
    55201 aacatgcgtc agaaacagtt agtattcaag ctgttttcta acatgattac cttgcttatt
    55261 ttgcttcgct tgccaataat tggtctgcgt agattttttc caatttttct tgaagagctg
    55321 ctttgatttt ttcagcatat tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta
    55381 cgctggcacg caatacagtt acttttccgg cacggttcca ttcttcatct gagaatccta
    55441 gttggttcac aaattgtaat gggctgttgc catagtctgg gatagcaaag tcagtatgtg
    55501 aagttaagaa ctcattaccg taaatgcttc ctttcacgta tgaagaataa tcatatacgt
    55561 catggttcaa tttattcatt gcaactaagt catcgttgcc tttttctttg aatacatagt
    55621 ctaccatatt gaaatctgga taagttaatg gatgaacttc gatttctttg tctcctactt
    55681 taaatgacaa gtcattcaag aagttgtaga aacgatgtgc accttcgatt gatgcaccca
    55741 ttaatttacc atatcctgtg acattcaaag gtaagacatg gtgtgctgcc caaacgctgg
    55801 ctgctgttgc ccctgctttt gaaccttcca agatataagc cccaagtaat gctgggatat
    55861 ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat aacgtctctc atgcggatat
    55921 ctttgatgac gataccaccg gcagaatatg gaacatagcc cattttatgt gggtcaatcg
    55981 ttacagattc tgcttcttcg attgctttat atgcactgtg tacttcttct aagatataat
    56041 ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa
    56101 tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta tccgccataa gcagcatcta
    56161 cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg acgcaatgca acgattttat
    56221 cgataccgtc gattgctcct tcttctgttg atcctacaac accgactaca cctaggattg
    56281 gtgttttttc agctgctaga ccacgaacga ttttttcaag ttcgttgatg tccatgcgat
    56341 agttgtggtc aactggcact ggaatcactt ggtctaaacc gataccgatg atgtcggcag
    56401 cttttagcca tgaatagtgt ttagtttgtg gaaccaacca ttttcctaat ttttctaaat
    56461 gttttccgct acgtgctgag tgagctttga tttcgtcgat tttttctggg acgctgtcta
    56521 acagattcat gatttcttct gttgacaagt tcattaattc ccaatcgctt tttccagcaa
    56581 ctagttcagg tgtcacttct ttcattgcaa gcggcaatga tttgatgttg cgggcatacc
    56641 aaagtccttc taagtttgct aaagaaccat cagcaacgat gtggccccag ccatctttat
    56701 agctcattaa ttttgcaaat tccattccta cttcttcttc catttggctt gttgcaggag
    56761 aagattcata ggcaacgtta ttaccattcc atagcatagc aaaattgtac gctagtaaag
    56821 atggcatcaa tgtttcagag ttcatatgac cccagtaacg tccggcatta tgccaaggaa
    56881 cggaatgggt acgcatccgc gctgaaattt ctgatagaac atcctttgtt ttattcacag
    56941 tgtgttcaaa actagcactg cttttttctt ctggcgtaat aataggcatg tcttgaggca
    57001 tgtagttttg acgccagcct aaatgttcat ccaccaattc attcaacaaa gctttataga
    57061 tttggccgtt ctcagctttg tccccaatga atagggcatt taggtttaga tctttcgaca
    57121 atgattcact catagtgaaa ccctcctaaa aaaatattta aattttgtcg agccctacat
    57181 tacacctgtt cttctattct gtaaactaag ttttggcctg atttttaatc atattttaat
    57241 atgtatcaat tatccgaatt gttcacactt ttgaaataat taaaaaaaac gagggaattt
    57301 acaaatgtaa actccctcgc aaagattgta ttaacgacaa ccagcgttct attttaggat
    57361 gggtttcaaa attggaatgt tgtaatagta tttccctttt tcaaggtaaa aacttactaa
    57421 attctaatca attactttgg ccaaaaagta atttttctta ccttttcgaa taaccacaaa
    57481 cttaccatcg aattcatcgg atggatttat gacaaaattt aaatcggtaa cgcggtcacc
    57541 gttaatactt atagctccgt ttgaaacatc ttcacgtgct tgacgtttag atggttcaat
    57601 ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg
    57661 cattttaccg aatccttgcg cgatttcttc cgcatttagt tctttaatat ttccagagaa
    57721 caatgcttgc gttatttttt gcgcttcttt caagtcttct tcactatgca caaatcttgt
    57781 aacttcttct gccaaacggc gttgtgcttc acgtttctca ggttctgtct ctactttttt
    57841 cgctaagtct tcgatttctt cttgagacaa gaaagtaaag aatttcaagt atttcactac
    57901 atcgcgatca tcttgattca accagaattg gtagaactca aatggtgaag ttttcttcgg
    57961 atcaagccaa acggcaccgc ctgcagtttt tccgaatttc gttccgtcag ccttcagcat
    58021 caatgggatc gttaaaccga acgcttctgc ttctggtcct tctaattttc ggattaggtc
    58081 taatccagct gtgatatttc cccattggtc tgctccaccg atttgcaatt gcacatcgta
    58141 tgttttatgc agatgcaaaa agtcgataga ttgcaggatt tggtaagtaa actctgtaaa
    58201 tgagattcct acttccaaac ggctggcaac gatatctttt gctaacatcg tgttgatgtt
    58261 gaagttttta ccgtagtctc tcaaaaattc aagtaaagag atttttgaca accagtcgta
    58321 gttgttcaca aatgtgatat ttgcatcttt accaaacaat tttctcattt gattggaaag
    58381 tgcgtccacg ttatgttgca cttgttccat cgtttgcaac acacgttctg ttttgcggcc
    58441 gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga atatatggat gatggcctgc
    58501 caattggaat cttttcatca tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc
    58561 agttggatca acgccgcaat aaagtgaaat gctcttttct tcaacgagct cgcgaagtct
    58621 ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt aattcatcaa taatattcat
    58681 tttactgacc tccgtgtagt ttttttatga aagcaggaac ctgctagcat atgtaattaa
    58741 ttatttcttc aggataggat aggaaataaa aaaagtccct tggcatataa gccaagggac
    58801 gaaatctcgc ggtaccaccc aatttgtatg tctagatatc caacatacct ctcgaacatt
    58861 taacgctgtc gcgtggcaat cactgccaaa gactcctgaa atgtaattcg ctttaaagca
    58921 tgtactggtt tgcagcgacc accagctttc tgaaacattg gctttaacaa ctacttcgtt
    58981 tcgatcttcg tcttgttatt attaatatgt gtctatcata gagcgcaact ttttctttgt
    59041 caacagaaaa aggatatttt ttttaaattc ctcatttttt cttttttgaa tccgctctaa
    59101 ttccgaacat tttattttca attattgtta aaagaaataa ctttttctgt attttttctg
    59161 caatttatcg agaagtcact ttactttgcc tcttattctt tcttcgttac aataaggaat
    59221 gggacaagta aaaaaatgaa aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt
    59281 attttaggag ccacttctgc tttaactatc gttattttaa ttctaagttt gttttttgtc
    59341 atccgatcat ctacaaaaaa agaaccaatc attcctgaaa aaaaaataac agaaaaagca
    59401 aaagaaccga cagatgataa gcaacaacta gttactcaag caaaaaatct ggctcaaagc
    59461 tacgattatg accaagcgat tgcgttattg gaaaaagata gtaagaacgg atctactgaa
    59521 acacaacagc ttcttcaaac actaaaaaag gagaaagaac aactgatcga ctggtctgat
    59581 cctactcaga tctctcatgt ctttttccac agcttgatcg tggacccgca aaaagccttc
    59641 cactctcaac aggcacaagg atacaaagat tatatggtga ctgttgaaga attcaaccgt
    59701 tcgatcgaac aactttatca aaatgatttt gttttagtta atttgaatga tttgatccaa
    59761 aaagacgaac aaggaaatct ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca
    59821 ttggttcttt cacaagatga tgtcagctat tatgaatata tggataattc cggttttcct
    59881 agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg tctataaaga gaaagataaa
    59941 gaagaaatcg gtgactttga catggttcct ctgatcgatg cctttgtcaa gaaacatcct
    60001 gatttttctt atcgaggcgc aaaaggtacc ttggccctga caggttacaa cggagtttta
    60061 ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa
    60121 gaagctaaaa aagtggcgga acagttgaag aaagacggct ggagctttgc ttcccataca
    60181 tggggtcact tgaatatgac tcaagcttca ctagaagatg taaaaaaaga caatgaattg
    60241 tggcagcaag aagttgcacc tatcctagga aaaacaaata ttctgattta tccatttggt
    60301 gcagacatca gcgactggca gccttattca gcagaaaatc aaaaatttgc ttacttgaag
    60361 agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta caccagcttg gggacaatta
    60421 ggtacggatt tctatcgaaa tgctcggatc aatatcgatg gcattcgttt tcaatctgat
    60481 ttagacggaa aaaatcctat tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa
    60541 gaagcacgcg aatctgcttg attttacgtc atttatgaac caaaaaagcc gaactgacat
    60601 tttatcgtca gtttcggctt ttttatgctt tatttcttct tcggtgcgat tcccttgatc
    60661 aggacttctg tccattctgc tgtatatgga agcagttttg cgtatatctc cggcttagaa
    60721 attccttcta ccattgcttg aaaatagagg atcaagaact catccgaata tttcaaatcc
    60781 acttttcctt ctttccgccc tcgatggaaa agatcaagca tgatactgaa actttcttgc
    60841 gtatacgcat ccatcgcgtc agaaaattct tttttttctt tttttgtata gtatgtcatc
    60901 aaatcgatat aaaattgttt gttcactcga ttgagataac ggattttgtt ttcactcatg
    60961 gcaatcagtg tttcttcaaa tggaagattt tccgcaatca acgctttcgc ttcttgccct
    61021 aaatcattca taaatgcaaa aaatacttca taaatcagcc cttccttgct gccaaaatat
    61081 ttaaaaatcg tcgctttact tacctgagcg ttctcagcta ccttatccat tgtcactcca
    61141 gcggttccta tgtctgtgtt catcaagtca aaagctgctt gtaaaatctc tttctttttc
    61201 tcttgtgctc tcttttcaaa accgttcatt tgtagagcct cccgtaattt taaactttta
    61261 taatatatat agttcaaatt tattctgagg tttattataa ctatctattg agaaagtatc
    61321 aagctagcaa ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt
    61381 actaaaataa taaaaaaagt tttataaaaa ggtataaaac ctttttatta aactatttaa
    61441 tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa
    61501 gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc
    61561 gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa atccacgacg
    61621 attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa
    61681 gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc aggcgatatc
    61741 tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa gcttcatgga
    61801 aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga tcctaagaaa
    61861 aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc tgctttggct
    61921 gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc tttgatggaa
    61981 acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat cctactttct
    62041 tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat acgcgaagga
    62101 acactcgatg aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc gaccatcact
    62161 atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca tgactttgtg
    62221 caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt gattttagct
    62281 gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct tgaggattta
    62341 ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt ttcacgctgg
    62401 tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat atggctagtc
    62461 ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac ttccggacaa
    62521 ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat ggtcggacca
    62581 actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg
    62641 cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag ccaaacgaga
    62701 aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg acgtcaagca
    62761 aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc
    62821 agctgtacga tgattttttt ccaagcggat tcgatcactg cagaaggtgc ttggcttttt
    62881 gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt tgctgcacaa
    62941 ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg tggactttac
    63001 cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa tcctttaggc
    63061 tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct ttatttgttt
    63121 ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg agatttgaac
    63181 accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct tttatccttg
    63241 cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat cacttttggt
    63301 ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt agaaagtaac
    63361 gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc
    63421 acgatcatgg tagtcttgat ttgtctagct gctattttac caattgctag catcaatcgt
    63481 ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa aatttctagg
    63541 aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt ggcaatcctg
    63601 attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc gaaagacatg
    63661 gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt agtgtttatc
    63721 ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc ttatatctac
    63781 ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc agaatggttt
    63841 tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt tgatctttcc
    63901 gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc tattggttat
    63961 aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat gctagcctcc
    64021 aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag tcttctaact
    64081 gctctttatc aatgatccag acttctcgat tctttctttt tacataacca ttctcctcta
    64141 ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca gcaagttctt
    64201 tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag tcaagcagat
    64261 agacagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc tgctgtactt
    64321 ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga taatgcagta
    64381 agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct agtgcttcgc
    64441 caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat ccgcccggct
    64501 ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact ttcatactgc
    64561 cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt tctccttttt
    64621 ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt actaagtgat
    64681 tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct cctccttcta
    64741 aacagctatt tctaataaag aaacataact tcccttattc caactgataa ttttctttta
    64801 tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt ctttttgtcc
    64861 cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa ttttttaaat
    64921 atttactttt tgaataatca aaaacaaacc ttcactcgca agccgagagt aaggtttgtt
    64981 tttcgcattg cttatttcgt gttgtctatt gaaatttttc cgcacctagc ttttgatata
    65041 gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg
    65101 gatagaagaa tagtttaccc atttgaaagg ttcggtctct catcgctttt ttcaactctt
    65161 cttgtttctt tgtatcggac aaatagtcag acaaagctaa gtggtcgtca agttcatgtt
    65221 ctggttgcgg ccatatgtaa taatccttga tatcgactgt tttgctccac tctttcagta
    65281 tcgcaagcaa ctggttatac gctgcaatgc taccatatcg ttcttcgctt ttataatgtt
    65341 ggtacatcaa atagatttgg tattgttttt gattcaacaa aactgctaga caggcattct
    65401 cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat
    65461 tcgtccagct ttcgatttta ggtttgctca tgccgtacat atcaggcaaa agtgcagctg
    65521 tctggagctg aatctctttc catttttgcc agtgttcttt atattctttc ttgatttgtt
    65581 caatcttatc tgttggatgg ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa
    65641 acaactgata atctgatttt ccaagttcca ttgtttcgcc tctttccgtt atcttttgta
    65701 ttctacctga attttgcgat atggacaaat agaggttgtg aaaaagctgt tctgcatcaa
    65761 gtcataagaa cagccaaaca aaaaactgcg atgagcatca ccgcagtttt attgaactta
    65821 aggttgattg atcacatcta aaagcgttga aaataaaatg gcactagaag cagctcctgg
    65881 gtcgatatgt ccgattgcac gctctcctag ataggaagct cgtccttttt tagctaccaa
    65941 gactttggtg tgttcttttg cctggtcaat gatttctttt gttaaattcc ctgccttcaa
    66001 tgcctctgtg actggcagcc aaacatctaa catcgtttta tcgtcagctt ctgcctttcc
    66061 tcggctttga atacccgctg ttccctgctc gatcacttcg cctaattttt cttgagaatc
    66121 aatagaatcg acctcttttg tcgctttcat catgttcatg aatgcagatc cgtataacgg
    66181 accagaagcc ccaccgactt tcgatatcat cgccatagag aatactttga atgtatcgct
    66241 gattgtttca ggatgatcgg cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat
    66301 gttatttcca tggtctccat cgccaatcgg ggtatctaga tcactgagat attgtttgtt
    66361 ctgattgatt gcctctgcaa attgcgtgag ccattcttgg atttcttgaa cggttagttt
    66421 catattatca cccttttatc ttgagatttt ttaccatgaa atcgtctgta cattgctttc
    66481 taaagaatct ttccattgtg tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat
    66541 atctagtgag gtcataaaat taccgacttt tcggaaaact acattgacgc ctctttcttc
    66601 caacagagct aaaacatcat tcatgaacac gaactgttcc atcaaaggcg tcccgcccat
    66661 tccgttgact agtacacctg cttcttttgg taaattcgag taatcatcta aaatcttagt
    66721 aacaagttcc tttgccagta ctttagaagg ctgcatcttt tctcgacgat agcctggctc
    66781 accgtggata ccaacaccat actcgatctc atcttctcct agttcgaatc ctggtttccc
    66841 tacttctggt acagttgctg ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac
    66901 cttttctccc agttctttca actcagctag ggaagcaccg tttcttgctg catcgcccac
    66961 gattttgtgt acaaggacag tacctgcaac tccgcgtttt ccagcagtat acgtactatt
    67021 ttcaacggcg atatcatcat ctacgatcac actttctact tcaatatcgt ccatagcagc
    67081 taattccttt gccatatcga agttcaatgc atcccctgta taattcttaa cgatcagcaa
    67141 aactcctttt ccactatcgg cttccttaat agccgtctga atctgatctg gagttggaga
    67201 agtaaacaca tcaccaagca ccgctgcact taacatccct tcccctacaa acccggcatg
    67261 ggatggttca tgtccgcttc ctccaccaga aaccaaacca acttgttccg ctttctgatt
    67321 cttggctaca acacgggaag attctacacg atgaacaagt tctggatagc tttttactaa
    67381 tccttccagc atttcttcga caatattgct tggatcattc atgatttttt tcaatttatt
    67441 cccatccttt ctttccggca ttttgtgctt ctgttaagat ttgctgagta tcatctgtta
    67501 taccggcagt gatggctgct gcaaaagctc cttccactaa aggcgcatcc accagatgat
    67561 aacgttgctg ctgctcttct tccaacatgt cttttgccag ttccgagttc aataccgcgc
    67621 taccgatatc cgcaaaaaca aaataactag ctgcatcgct tgattcattg actgcttcta
    67681 cgattttcat cgggtctgat ccgatctcgc catttattcc gccgagagaa aaaatttgca
    67741 cctcttcgga tttttgcatc tgctcaatca tttctttaat accatctgtt actttttgac
    67801 tatgagaaat aagcaaaata ctttttttca caagaatctt cctttctttt acatagttgc
    67861 taacgattgg tttcgctttc attaatgaac aattcaagta tataataaat ttctaatttt
    67921 gggaaaatat gtgtagtgct tcttgaagtt tgttatactt taattaaaag agaggagtga
    67981 tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa gccgtagtag cttataaagg
    68041 gatcgttttt ggtgaggtca ttactgggat ccatgcattt aaagacttgg aggcggggtt
    68101 gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga
    68161 agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg
    68221 cgtaaaaatg gactatgaag tattaggcag cgacaatggc atgctgatgg tcacatgtag
    68281 tggaacagct gtctccacga gataagtagg ggacatctgt tttaataact aaagagagat
    68341 gggacatgta gtggcactca agaattagat ttttcagtct aacttcgagg tactctacct
    68401 tcccatcctc tcttttttat ttctttttgc ggatgattcc gtaagctgct gcgactaaca
    68461 cacccgcccc tgctaaggaa taactgacag aagctgtttt ttcatttgtt tttggcaaag
    68521 tctgacttgt tgatttggat ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag
    68581 aggtcactga tttttctcct gccgaatgca aaatatcttt tattcgttgg aaactagctg
    68641 ctaagtcagc tttttttgct tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc
    68701 cgtggattgc ttgactggcc gctgtgatat ccgcttgagt ttgacttagc tttccagaag
    68761 ctgtttgagc aacctgattt aattgctgat tgaattcttt tgcttgttgg atcttcccgt
    68821 caagatctgt ttggacagct tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt
    68881 gtgcacggtc atttcccact ttatttttaa gatcagctaa atcttgatcg attgctgctt
    68941 ggtcttcttt caatttgttt tttgcagctt ctaatgcttc ttcgagttct tttcttttat
    69001 tttccaagtc ttgcaaatga tcgatttggt cagaagaggt agaactattt attgatgtat
    69061 cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg tacattggaa gtcatttctg
    69121 tttccgaggc accgttttca gcaccagaag agcttatggt tgttgaaggt tctgtttgag
    69181 caggttcaga agtttccgca aaagctgtct gtcccaataa gaaaatactg gaaacagcaa
    69241 ttggccagat taacggcttc attctttttt tcatcacttt tcccatcctt actacttttt
    69301 aactaatttc ttattataag caaaaatgtt tggaagaacc ataaacacaa caggctgttt
    69361 gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc gggcttttcc atgtcccgct
    69421 tccataacct aaaatcgttt tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt
    69481 cactatccag aacaaaagca tatatagtgg tgcgaaaaga aggtatttca attttcttcg
    69541 tcgatcatct acaatcagcg aagctagcaa ctggaaaaat cctgcgacca tttcaaagca
    69601 gatgaacaca aaactgaatg taaaagtaat ataaatctgt tcgtagtttc cttgatatac
    69661 ataatgcccg attaatccag caaaaagaac tacggaaata caaaagaata ttgaccagac
    69721 aatactcaat gtttgatcaa tgaagatgat cgttcttcca atatgtttaa aaggatggag
    69781 catcactttt ttgaaattcg tcagccagac ttctgttcca ccttttgccc aacgttttct
    69841 gtgtcgatat agcatcttca acgtcacagg tacttccatg aaaaagataa tctcaggagc
    69901 aaaaacagat aaccagtcat ttaactgatg atcccaagca atactgatgt cttctgtcgc
    69961 tcgatcttgt ctgaatccgc ccacatcgat caaggcttct tttcgataca tcgtatttgc
    70021 tccactgtac gcataaagac caccgaaaac agctgactgt gttcgtttaa tgatgcccac
    70081 gatacttgag aattcaaccg tttgagattt tgcaatcaat ttggtacgat tttggacatc
    70141 catatttgca gtgaccgcag caatatgtct tgcaccaggg cgaatgaaat aattcacata
    70201 cctaatcagt gcatctggct ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc
    70261 ttttgcaaag gcaagtccga tattaaacgc gtgggctttt cctttatttt tttcgatccg
    70321 aaccacacga agattagcgt attttttcat cagtctagct aatatttctg gtgtttggtc
    70381 cgttgaacca tcgtctgtca ctaatacctc atagttgtga taattcaatt tcgtcatcaa
    70441 atactcgatt gtatattcga tcacaatttc ttcattatgg gctggcacca tgatcgtgat
    70501 gaacggttcg acactcaagg gaacatctac ccattctttt tgcttatgtt tgtataaaaa
    70561 cacataacac cagacaccaa taaacgatac aaatccgcct aaaatcggat aagaaacgag
    70621 aaaaaaatac ataatagaca aacaagtatc taagaataaa ttacctgtat gggtgattga
    70681 gatcatttca gttccacccc attttcttta tacaaatctg caatgaatgt cgtatctaga
    70741 tttttctctt cggagacaga ataaaaacgg accgtttctc tctcttcttt tgttccaaaa
    70801 cgttccgtat acacttcttc aagtaattct tgtctttttt tcaatttttc atcttcatat
    70861 tcaaaagatt tatttacttt tttgtataga tttcgattgt tccacctagt caatacaaat
    70921 gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa atacagcatt
    70981 ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat
    71041 gggaaaaaga cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac
    71101 gtttgccaaa tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt
    71161 gatgcaactt cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga
    71221 cgtaggagtt taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta
    71281 agaaaaagaa caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt
    71341 tgttcttttt attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc
    71401 attcctctag atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt
    71461 tcaaatggat ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg
    71521 atttggcagt tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt
    71581 tcctcttctg tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca
    71641 gctagtattt tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct
    71701 tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc
    71761 tctgtgattg ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga
    71821 tggtagtcgt cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc
    71881 tgattctcgt cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct
    71941 aattgtgcca gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca
    72001 tttacctttt caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta
    72061 cgtgtgggtt gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac
    72121 tgatgctcat ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt
    72181 tgtaaaagtt tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta
    72241 ccgaataatt ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga
    72301 ctttttgctt gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct
    72361 tgaagcctat cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt
    72421 tttttagaaa ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct
    72481 aaaaacaaag gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct
    72541 gtctgctccg atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt
    72601 tcctctgctg aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca
    72661 cctaatgatg gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc
    72721 agcaagtcat cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc
    72781 atgatagcag aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc
    72841 acatcccgaa tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca
    72901 ttggtctcat cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta
    72961 tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc
    73021 gtatcgattt tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa
    73081 agttcggcaa ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa
    73141 tttgctgtac cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact
    73201 tttccaaagt agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt
    73261 ttctgtttga tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta
    73321 tccaggttat ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac
    73381 gactggtttc cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca
    73441 attagctttt gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc
    73501 atacacattc tcctcttttt ttaaagtggt ggaaccgatt agtttacctc ttttaagagt
    73561 cgaatgcaag ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac
    73621 agtttgatat aaagaaaatg caccatcaag gttttttacg tggtagccag actgcttcaa
    73681 gattctttcc gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg
    73741 gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt
    73801 gaatttgccc ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc
    73861 tagttctgtc ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat
    73921 tgctgcatat cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt
    73981 caattctaat tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat
    74041 atcgatccgt ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt
    74101 ctctggatgg aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg
    74161 atcttttcct gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc
    74221 acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc
    74281 gccttgattt cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt
    74341 ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat
    74401 agcatccccc accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat
    74461 acccccgcgt atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac
    74521 accaatggat aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg
    74581 tttcccgtgg tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg
    74641 agacaattct ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg
    74701 tgcttgttcg atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa
    74761 tccgatgaat cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat
    74821 cactttatcg atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc
    74881 tggaatcgat ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc
    74941 aatgtgtttt tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt
    75001 cacttcatga tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg
    75061 gacaagaaga ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt
    75121 tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg
    75181 gcgaagccgt gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat
    75241 cttatttccc tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta
    75301 catcataacc agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac
    75361 aaatcacata gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa
    75421 catttttagc tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta
    75481 acgctggatt ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt
    75541 gaaatagcaa attcattttc tgcacctcat ttacattttt ataaatacct cacggggtat
    75601 attaaacagg agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt
    75661 tttatatttg cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat
    75721 gtgttatgga gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa
    75781 ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat
    75841 taagcgctgt tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga
    75901 aaaattgctt tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag
    75961 cgatcaagat gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt
    76021 gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct
    76081 ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct
    76141 gccaatgcag aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg
    76201 cgtgctaatc atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt
    76261 gtccttgatt tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta
    76321 gaggttgcga cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac
    76381 ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac
    76441 agccaaacaa aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca
    76501 agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag
    76561 agaaaaaaac gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt
    76621 cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt
    76681 aagaaaaatt ttgtgaaaat ggatttctac atttttgcat aaatttggct taataccgag
    76741 aaactggctt ttgaataccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt
    76801 attagagaaa caaacggaaa aatagctgct catattttgc cgttttttga gctaataccg
    76861 aaggtcagct gattgaacac cgtttaccaa gaggttgtga gcctaccatt taactccgaa
    76921 tcacaagaaa taatttcaaa aacaacttct cctgtttttg gttcggttgg cttattatcg
    76981 agaagtagct actgggagtc tatttatcaa aaaaaggaat gcgacatagg tcacacccct
    77041 tttttatttt ctttgaaaca tctttcggta ctcgacagca tacaagacaa aagattgtaa
    77101 aagaccgtaa gtgatgacgg caaaaatgac tctcacccac agcgttggta attctgggaa
    77161 aagcagcaaa aggatcatgc caacatagat gacagcagtc gctactttgc cataccactt
    77221 cgctccactt aatttgatat ttttagatag cccgattaat ccagctacaa acataaagcc
    77281 ttcttttatc aagaatagac caaacaacag ccaaagccac ggtcgatacc atgccataga
    77341 caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct tccctaagtc
    77401 tgttacctga ttatatctcc gagcaatgac cccgtctaga taatcagaca aacctgagac
    77461 aacaatgacc gctcctgcta tataaaatga ctgtctcagc acaaaataaa gataaatcgg
    77521 cagcaaaaaa atacggaata tcgataaaag attcggaatc gtttgtattt ctttacgcca
    77581 gtctgtgctt aatttcattt tatcgcccta cctcctcagc actcttttct ttttatttta
    77641 gcttacacgg ggaataaaac caatgatttc actgttttta tttttcttta cagacactga
    77701 tattatgagc taatttgaga taatcacttg ctgttttcat cgtttactct tctttcttcc
    77761 tgtcgtttgc gcatttcttt tactagtact ttatgagctt tttttgatcc tcgctctagg
    77821 tcacgctgcc gtttacgtgt tttataatcg ataaacagac tgtctaaatc gtaacctgtc
    77881 gggtacagat cttctgctga acgcaccaga cgaacacgct gacgaggcac gtggattatt
    77941 tttttgtcta aatatacatc aatcgtatct tcacctatat cttcataaac taacgcttct
    78001 ttttggtatt cattgacaaa tatccgatca ccttttgcaa agagctgacg ttctttttta
    78061 ttctccttcc tatttgtaac tgctgtctta ggaagaaatt cttttttctg tgtccgatag
    78121 gctttttccg ccaagtaacg ctctgcttga tggatcaatt tcatcgacat atccatttta
    78181 tgagcaatcc acaaagcttg gctttctcct gtttcttcca acccgcaact gatatttagg
    78241 acgaagtgct tcccgatcga aagccatagc agccggcaca aaatcttcat gatctccggc
    78301 gaatttcttg atttctccat aatgggtcgt ggcaatgatc aatgcccctt gctcatacat
    78361 agattccatg attgcgatcg ctagtgcagc cccttcattt ggctctgttc cactgccaat
    78421 ctcatctaaa agtaccaacg tattcctttt tatctttttc aaaatagcag cgatgttttg
    78481 catatgacca gaaaatgtac tcaacgcatt ttctaaattt tggtggtcac cgacatcgac
    78541 gaaaatctca tcaaagacag caagctctgt cccttctttt gccggaactt gcataccgaa
    78601 catcgccatc aaagtcaata aaccgactgt cttcaacacg actgttttgc ctccggcatt
    78661 tgctccggtg ataatcaagc ctcgatagtc tttaccgatt tcaacatcca atggtaccgc
    78721 atgctcaggt aaaaatggat gtctgccttg cttgataatg atatgttccg atttattgat
    78781 tctaggtgtg cttccattga tttctcggct aaattttgcc cgagcaaaga tgatatctaa
    78841 caccgtgatc gtttcgatca ataaatcgat cacttcttcg ttttctgcga tagcaccagt
    78901 caatgccgcc aggacttgat attcttcact gatttcttca gcttttaaca actgatagtg
    78961 ttcattcaat ttagaaatca cagttggttc aataaaaacc gtcgttcttt tatttgattc
    79021 atcgataatc gttccatcta ttttattttt atagctggct ttgataggaa tcgtatagta
    79081 ttcgcctttt tgaacaatca ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa
    79141 gaatttcagt aattttgact ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt
    79201 acgcaaatta cgtgacgcgt cgtcactgac tttattattt tttattttct ggtcaatcaa
    79261 ctcttctaca tttatcaaat caggaagatg tttgctataa gcaaacaaca agggtgtctg
    79321 gtattgattc ttatcaaaaa actttgtgat catccgactg cttctaagaa aatccgcata
    79381 ttcgatcaaa tcggttggct gcagaatcaa cccttttttg acttgttctg tcagtgcatc
    79441 gatcctcggc aaccccataa atggaacatg ctgactgctt tctaagatca agcgtgcttc
    79501 tcttgtttct tcttgacgat ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct
    79561 gattcgtttt ttgctatagt ttccaattgc tcttgcttgg atttcctttt tgatccgatc
    79621 aaattgtaat tgactaatca tttcgttgtc catattctct ccttttcttg aagagatacc
    79681 tgtcccaaaa taaataaggg cctagagata aataaaagga tagaacacag cttaaaaaag
    79741 tcatgtccta tccttattag aatacaaaga tattgttttc ctgacaggta ccgtttcata
    79801 agcacaccaa ataaactttg tttattggct attcgactta ctgattagat accagctaca
    79861 ctcacaaaac ataccccatt cttctaggtc ttatttattt tccacttaac tatagcaaga
    79921 cacattttgg ttgtcaaact ttagtatgtt ttatagattt acaccattaa tgaatagaca
    79981 cctaccgcga taattgctcc gatcattgga cctgcgattg gtacccatga atatgcccaa
    80041 tctgaagttc ctttattagc tattggcaat accgcatgtg ctaatcgagg accaaagtcc
    80101 cgagcagggt tgattgcata tcctgttggc cctcctaatg aaagaccgat ggctaagatc
    80161 aatccaccta cagcgaatac gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc
    80221 aacccaagaa ctaatacaaa tgtaccaatt aattctgtga tcacatttgc tggataattc
    80281 cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc
    80341 caatgtggca aataagaaag ccaaacaaga atggcccctg caaaagctcc taaaacttgc
    80401 gcaatgatga aaggaacgac aagattccaa ctaattgccc ctgtcatcgc catagctaga
    80461 gagactgcag gattcaaatg agctgggccc atgaatccag aaatatagac agccattgtt
    80521 acagcgagtc cccaaccaaa tgcgatgaca atccagccag aagcctgtgc tttacttttg
    80581 tttaaattca cagccgcaca cacaccatct ccaagcaaaa ctaggatcag tgtcccaata
    80641 aactccccca tgatttgagt catatcactg ttcatttatt tctccccctt tagttcccgc
    80701 agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct
    80761 gtccattcaa aatatccagc aattgcatcg atcacaggtt gcttgatctc atcaagctga
    80821 tctcgttcaa acaacagatg atttgtacga cgaatgagat aatcgcctgg ggccaaaacc
    80881 atttcttctt ctaaaccata acgtaaccga gctgactcag cgagactcaa accaggataa
    80941 ggtgccattt cttttgctaa ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg
    81001 atataagtag catcttcttc ctctaaaccg gctgctactc cttcttttgc taattctgtc
    81061 actgtttctt ctaatttagt tggatcgaag tttccacctg aaatctgata ttttttagag
    81121 tcgatttctt tcgtctctat tccgtattct tcttttaata attggcgtat caatcgcaaa
    81181 gctccttctg ccatcttacg gtaatcggtg attttcccac cagataaagt cagcaaacca
    81241 tctggttctc tttctaaaga actacctctg gaaatcgtag aaggtgcttt ttcatcacgg
    81301 ctgttttcca aatggttcaa cacgtcttct acttcagcac gagaaacttt gttttcctta
    81361 tattcactta ctgtatcgac aactttattg aagctcttgt ctgaaatcga tccattatct
    81421 ccaccattgt aatcagaacc agaattacca atcaacagtg gacgaagtcc tgcccagctt
    81481 gcttcgatat cattcaatgt gatatttgct tctggatagc gatgattgat cacatccaat
    81541 agataatcta catcttcttg tgtgactttc ggatccgtaa aatctccttg gtaatccgta
    81601 tctgtcgtac caaagtaagt cttgttttct cttggaatag caaaaaccat ccgcccatct
    81661 tgctttcctg tatcgaagta tgtgggttgc ggtacaggca gttttttcgc atctacaact
    81721 aaatggatcc ctttagttgg acgcatttta ggagagactg gacgcgtaaa attcaagttt
    81781 cgtactttat ctacccaagg accactcgta ttgatcacta attttgcttt gatttcgatc
    81841 acttcatctg tcagcaggtc acgagctttg acgccaacga tttgatcgcc atcatataaa
    81901 aagccaaccg ctttcatttt acttactaga taagccccat cttcggcagc ctttttgata
    81961 ttatcaatca ctaaacgggc atcattgttg cggaaatcta gatagacacc cgcgcctttt
    82021 agcccttctt ttttcaaaaa cggttctctt tccaatactt cttctggtgt cagcgtataa
    82081 ttctcatatt tggttcctgt cacatttgcc agtttgtcat aaaggtccat tgctactttg
    82141 accgagaaca tattgaaggt tgttgctcct tcgccctcat agattggcaa aagcattgga
    82201 tctggttttg ggatgtgtgg ggcaatactt tgtacgactg cacgttcacc aactgtgtca
    82261 gctactactt ccacatcaaa tgtcttcaga taacgaatac cgccatgaac aagtttggtc
    82321 gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct
    82381 gatgctgctg actggactgc cacaccggca ccagtgattc cgccaccgat gatcaacaga
    82441 tccaattctt cttgctgcat tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata
    82501 tggatttcct ccttattcaa atgcttgggt agcttttact gcttttttcc aaccgttgta
    82561 aagtttttct cggcgctctt cctccatttg aacttcaaat agcttgccag cttcataaaa
    82621 ttcgcggatc tcgtctgtat ctttccaata accgacagct aatcctgcta aaaatgctgc
    82681 ccctagcgcc gttgtttcca agttttctgc tcgttggatc ggtacattca agatatctgt
    82741 ttggaattgc atcagatatt cattgtttgc tgcaccgcca tctactttca atacaggaat
    82801 cttgattcca gtgtcatctt gcatcgtatc gatgatatct ctcacttgat aagcaatcga
    82861 ctgtaaagta gctttaatga tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc
    82921 ccctcgtgct gcctgatccc aataaggagc acctagccca acgaatgcag gaacaacata
    82981 aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa
    83041 catttgaagt ccatctctca gccattgaat cgaagaacca gccacaaaaa tacttccttc
    83101 taaagcatag taaacctttc catttatgcc gtaaccaatt gtcgtcaata aattattttt
    83161 ggataattgc ggtttttcac cggtattcat gacaataaat gaacctgtac cataagtatt
    83221 cttgaccatg cctggttcga atgccatttg tccgaataat gcggcttgct ggtctcctgc
    83281 catccctgaa attggtgctt cgcttccata aaaatgataa ccttgcgtat aaccataaac
    83341 ttctgagtta gaagtgactt ttggtaacat cactcgagga atatttagta aatctaaaat
    83401 ctcctgatcc caatctagct gatggatatt gaacaacatc gtccgactgg cattcgagta
    83461 atcagtaaca tgtgcttgtc catcggtcag tttccatact aaccaagaat cgatcgttcc
    83521 aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct tctacatgat caagaatcca
    83581 gcgtatcttc gttgctgaga aataggcatc gatcactaac cctgtttttt cgtggaaaaa
    83641 ttcttgatag ccttcatttt ttaagttatc tgcaattcca gctgactgtc tggattgcca
    83701 aacaactgca tgatagattg gacgtcctgt atctttttcc catattactg ttgtttcacg
    83761 ctggttcgtt atgccaatcc cagcaatctg atttggtttg atccctgatt cgatgaacgc
    83821 tccggcaata actgactgta cagaattcca gatttcattt gcatcatgtt ccacccaact
    83881 ttcttgcggg aaatattgcg tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt
    83941 gtcaaagata attgctcgtg agctagttgt accttggtca atcgccatga tatacgttga
    84001 ttctgtcatt gtgattccct ccattatatg caaacgcttt ctttaactac tcttatcata
    84061 ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt tttttctttt tttgacagtt
    84121 ttttattatg aagctatttt ttaatcaatt cttttctttt ttcagcgtct ctattcttct
    84181 tttcaatcga ataatgtcgt aaggtgacgc aaattctgac agataatatt cacactgttt
    84241 tttgtttctc tggcaaaatg tgatcaccaa atcatattct ttaccttctt cataacttcc
    84301 aatctcaata ccgccgattc ccctcagttc tctgatcaga tattgttgaa aagcaatacg
    84361 ataaatcggc agactttcta aatcgattcc aatcactact ggttttgcaa tataaaaatc
    84421 gatcatcaaa agaacattgg catactggat catcaggtaa ctaagctcac tatcttcagc
    84481 tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa
    84541 ggaatgcccg agcattttct gctgctgttc caaaagccga tcacgctcat agacttcgat
    84601 ctttccaaca aagaaaaacc attcattgtt gatctgggaa atttggtagc ctactgcctt
    84661 ttcttctttg attttcaaac gacgaggacg ataatgccat aacatcgttt cccgaatgta
    84721 agtatctaaa acagcggttg gtaacttttt gctccgtgtc aaatcatact gataaaatat
    84781 ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa aacatcattg tttcgtatac
    84841 attcagttct gctgctgttc gactaagatg catcacgatg attggatcga ttgcttgata
    84901 aagccggtcc gactgataga gtaatttctt ttctttcaaa gttgcgtacg tacttttttc
    84961 attcaatagt ctttttttcg taatcccgag ccagcaagca atctgtgctt ctgctgatgc
    85021 cgtaaacgtt gtttctaata cacggtccaa tcctagaatg aacggtcttt tttctggtgt
    85081 attttgcaaa aaaagcggtc gctgatcttc cgggatatat tggaatagtt cataataaaa
    85141 atatcgtatc tgtagctctt ctccgtataa ttgaccgttt ttgatttgta atccaaattc
    85201 ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca gaagactcac tgatattaaa
    85261 cgctgtcgct aatcgaacga tggatacttg ctgatgttct aataaataga cgagcatctt
    85321 aaacttcaag ctttcctgca ataaaaacaa tagaattttt tccaaactta catcttcgtc
    85381 caacacaagc agcgcctgat actcatttcg ctgtacttct actttttttt ccatcattcg
    85441 gccaatcaat tcaatatctt ccaaatattg atcaaacgag gcttttgaca gttggacatg
    85501 ctctctcaaa ccattgatag aggctgttcc cccttgcaga acaaggtttc gcaaaatcag
    85561 gatctgtaac tgctcttttc tttctaataa tgcttcaatc ttcataggca gtattcgcca
    85621 cctttgacca caccatatct ggcacacggt attctggctg gaaaaatagc tcatgttctt
    85681 ctaactgttc aaacgtccat ccttcttcta ctgcaattgt cagttggtca agaaggtgga
    85741 acatctcttt tttgctttgg agctgtgctc caagaatctt tttttcagaa tcaaatagaa
    85801 atttgagttt aactttgctt tgatttattg ctgtaagctt gtagtctacg tatcctgttt
    85861 caattttttg gggataaaaa atgctctctt cttctgtcaa acctacgctt cctaagaaat
    85921 agccaaacca ctgattgccg actggacgat aggttccctg atcctttgtc tgtgagccac
    85981 taattgtctt gctgacattt cgtgctgtac ggacagcgtt atttactaga gaaacataca
    86041 ttttttcatt tgttggacga aaagttactt ggatcgcatc gccaatcgca taaacgtctt
    86101 tttgagacgt ttgcaagtag tcatctaccc agatcgttcc atcatcattc aatgttaatt
    86161 gttcttccca catctgattg tcgggacgag aatgattagc cagcaaaaca agatcatagt
    86221 tctcctttct tttttccgtc tctacaatag cggtttcatt ttcggtcaaa gacacaactt
    86281 gttcttctaa aaaaagtgaa agcccttgat ttttcatttc ctttactaac ggttctgtca
    86341 tttcaggatc tagataacga aaaagaattg actttcggct ttcgtaaaga tggatttgtt
    86401 tttttcttga tgccaaccct tcagctattt cgattcctgc ttgtcctgca ccaataattg
    86461 caattttctt tgcattcatg atcttttgct gaagagccgt tacgttttgt atctttttaa
    86521 ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg
    86581 ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc taaaacgact tttttttctt
    86641 tcaatcctat tactgtcttt tctgtgtaaa catggattcg atagcggtca gtcagttctt
    86701 ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt tccttttaat atcaaattga
    86761 acccgctggg aatccatcct aacttcgatt gcttttcaat caaatatatt tcacttgttg
    86821 gcatcaattg gcgaagataa atagccgcat gaatccctgc aaacgacccg ccgacaatta
    86881 caatgttcat ctgcattcct ctttcttata aggtgttcta ctgctaactt ctcaatcaga
    86941 aaacacagta ttcataactc gctttcaacc ttatgataca tagcgatgca gcagaatcaa
    87001 ccctctccat cataccgtgt tctaggtctg cttctcaata atgtactagt ggacctaatt
    87061 gaagagtatc atttctgaag agatgtttca aaaaatgtac tcttataaaa gaaaaaaact
    87121 cgccttttac gtacctctgt ccaaatagga aacagaaaag aacgaaaaga cgagttttag
    87181 cgtgtgtaat gtaatagtta gaatagacac atcatttatt tttaattctt taatgcttca
    87241 acaactgctt cgcctaattg atgagcagat tgaggatttt gccctgtaag aagccgccca
    87301 tcaatttgaa ccacttctga aaaagctgct ccagactgat agtttgctcc tttttctttc
    87361 aaagcatctt ctgctaagaa tggtacttca ttcgttgtac catttgcttc ctcttcttca
    87421 ttactgaacc cagtcactgt acgtccagct aaaatagatt ttccagactc atctgtaact
    87481 ggcaacaatc ctacaacacc gtgacaaaca gctgagacca gcccttcatt tcgatagatt
    87541 tgtgaagcga ctttcccaag atctattgct tctggaaaat cccacattgt tccgtggcca
    87601 ccagcataat aaaccgctgc ataatcattt gcatccacat catcaggacg taaggtattt
    87661 cccaaggccc gatttctgaa atcttcatct tcataatagg cccaatcgat ttcatccata
    87721 ttcaaaacac tttgcggttc taaaggtaca tatccgcctt taggactgac aaaatctgca
    87781 tcgatcccat tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct
    87841 gtaggacgct cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt
    87901 ttcccatcct ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata
    87961 atgggcttgg caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat
    88021 cctcacggtt ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa
    88081 ttgctgcaag ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac
    88141 cgacaccaac taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt
    88201 tatcgttgta tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat
    88261 aatctagttt tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac
    88321 ctggtgtttc atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat
    88381 attgttcaac cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt
    88441 ccaagctacc gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga
    88501 tcaaatacgt atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac
    88561 gtgtggcttt gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg
    88621 gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag
    88681 ataaagttgc tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc
    88741 cttctccttg gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga
    88801 tgctgagttc acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa
    88861 cagcaccaag acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc
    88921 cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa
    88981 ctcctgaagg taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc
    89041 gctctgatta acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca
    89101 actaatttgc ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc
    89161 tgggctaatt ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct
    89221 tcccagctac catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat
    89281 gacaagcgac cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa
    89341 cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca
    89401 tctttttctt ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta
    89461 aacgcttcat cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga
    89521 cattcatcta acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata
    89581 tcatagtatg atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct
    89641 agatttgaaa aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc
    89701 cttctggaac accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa
    89761 aaattttgtg aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact
    89821 ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa
    89881 gaacagccaa acaaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt
    89941 cagctgattg tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca
    90001 aggagcaatt tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg
    90061 agttggcagg agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca
    90121 gtcccttatt tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca
    90181 atcaaatgat tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac
    90241 atagatatat ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca
    90301 gccccacgac gtataaccaa ataaatctac tccatctttg atagcttcct tcatctgctc
    90361 aatatgctgc ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac
    90421 tttgtctata gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata
    90481 taattgattc aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga
    90541 tggaagatac ggatttttaa ttcctccagt tattaagttt ccttggaccc cttcccaatt
    90601 ttctggatga gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg
    90661 gtaggctgct aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc
    90721 aaacattcgt tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa
    90781 agaagttctt tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac
    90841 aagaagctga tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc
    90901 ttttgtagct aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc
    90961 caaatgggga tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca
    91021 gttgatctca ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt
    91081 ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt
    91141 aaccaaatat aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt
    91201 tagacattcg tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc
    91261 atcaccatga ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat
    91321 ttcagcaaat aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt
    91381 aggataataa tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat
    91441 atgccattga tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga
    91501 cacagataat cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc
    91561 tccgccccat aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt
    91621 tttcttgttt gattattttt cttatgacta gtaatgatat ttttgaatct actactattt
    91681 gtaacaataa ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact
    91741 tccatcaata aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat
    91801 ttgccattta aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca
    91861 ctgactaccc caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagaa
    91921 ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca
    91981 aatgttggat cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac
    92041 actatatcct ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc
    92101 atacaggaac tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca
    92161 atataaatcg ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg
    92221 gcaccagcta ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta
    92281 tatttaagag tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat
    92341 gcagataaag ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact
    92401 gctcctgctt gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag
    92461 aaagaaagat tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac
    92521 acttgccaaa atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct
    92581 aaatatccag ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa
    92641 ataattggga ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg
    92701 tgcaagcttt tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct
    92761 ggaataaccg tacttgtata ccttgctaga atcacgggca tccctagaaa tcgcaaggtt
    92821 atgctatcac tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata
    92881 gcgatgaaac gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag
    92941 aaatagaaaa atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt
    93001 aataaattca aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag
    93061 acgcctaaag tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct
    93121 tttttctcag ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat
    93181 aatacgtcat acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct
    93241 tcgactacag tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta
    93301 tcttttaggg taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg
    93361 cctccgacaa atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc
    93421 ttgttaatta atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt
    93481 ttctccattc aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg
    93541 tttacttagc cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta
    93601 tagtcaaagg agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc
    93661 tcaagctgat actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa
    93721 gtctcggcat ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg
    93781 gaatctatct ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt
    93841 tttgttttct ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc
    93901 caagattcaa actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata
    93961 agctcttgct cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg
    94021 aaaagataca ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc
    94081 tgttttttta gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct
    94141 tgttttcgaa aaggattcaa cgcttcggca agatacaaaa caaaataagc gctttcagct
    94201 aaattgggac gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa
    94261 ggataacggg agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc
    94321 ctcgttttca tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa
    94381 acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt
    94441 tctttgtttt tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt
    94501 gcttgttttt cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg
    94561 gtctctacca tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt
    94621 cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata
    94681 taattatatg atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa
    94741 aaaattgtac tgatttgttg aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca
    94801 agagataaga aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac
    94861 gaaacttcca gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt
    94921 aatgatacgg ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac
    94981 cctttttgaa acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag
    95041 attttatgtt tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct
    95101 aaaaaataac ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc
    95161 atatttttga tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct
    95221 tcgcttgtta tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta
    95281 tttaagtcca tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa
    95341 tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt
    95401 tttaccatta gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct
    95461 aaagcttccg ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat
    95521 ttcccctgtt cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg
    95581 gcaaccatct tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg
    95641 ttttggatcg tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc
    95701 aactatgaat aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc
    95761 tagaggatca attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc
    95821 gaaaccattt gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct
    95881 acgataataa ctaaatgcaa tataatctgc cgtgttagct tgcaagcact gttcgtctcc
    95941 atctaacatt actggtgcac agtcttcttc cttccatgtc ctgtaaatat aagctggata
    96001 atgccctcgg cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc
    96061 aaatacattc tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat
    96121 aatacccatc ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc
    96181 tagaaacata tgatgacagg cttgataaat cacttttttt gttagtgtag caatgggttc
    96241 aagtttatct ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc
    96301 attattgatc tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac
    96361 ggtctgcgca taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc
    96421 tcctaccaat gctaaggggg tttcataatg actaagagta attactggct cgatatcata
    96481 tcgatttaat gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg
    96541 ttcgtctcct cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc
    96601 taactccttc agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg
    96661 agaaagataa tagagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca
    96721 ctgaatacta ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt
    96781 gccgtcttcc aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa
    96841 attttctgga aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac
    96901 ttgaagtata gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga
    96961 aaaaaattga atgctactct tcatttttaa tatttatata taacaaatac ataagacgat
    97021 ccccgaaaga aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa
    97081 atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg
    97141 acacaataac ccttaaagct tcacgtggaa tagtgaacgt ctgcttttca ctggacatat
    97201 tacaaataac gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag
    97261 gcgtcttttc cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat
    97321 cgatcaattt cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat
    97381 tgatttcttt ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat
    97441 gttcacgggc gttccattgc ataggcgtcc tagcattgtc tcttcctttc gccttgattg
    97501 attccaagat ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg
    97561 tctcaatatc ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt
    97621 ctccttgata gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg
    97681 cactttcttt tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt
    97741 gattattcca aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata
    97801 atactttttt cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt
    97861 gctgatctaa tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat
    97921 tggaataaag tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat
    97981 ttttgtcacc aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat
    98041 tacttgtgat ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc
    98101 ccccaatacc tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt
    98161 tatttttcca attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg
    98221 ttttttcatc caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt
    98281 ttccattgac cggatcacgc caaatataat agtcccgata aggattttct ttatttttac
    98341 gggcctcaat gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga
    98401 tacggatatt tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga
    98461 attctgccat gattgcttga taattactga tatcataccc attgtcatca ttcggagact
    98521 cgtatactgg agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg
    98581 aaataatccc ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat
    98641 agatttgata aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa
    98701 tggatgtttc tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac
    98761 gctttcaaat agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga
    98821 ggaaattccc tcaactgttt tttgaaagga ttgatttatg cagggaagaa tgctgatttc
    98881 acaacttctt tataaacggt gttcactcag ctgtcccttg gtattagctc aaaaaacggc
    98941 aaaatataag aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt
    99001 cacaacctct ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac
    99061 catacatgat tggagatact tcttttcctc ttttagctgc taacatggta attggataga
    99121 agataaaacc taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga
    99181 tcaagaatga agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg
    99241 ccatcaatac tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata
    99301 gtaatggaga aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac
    99361 ctgaacgtcc tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag
    99421 taccaaataa ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac
    99481 gaggcatctt gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg
    99541 ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat
    99601 tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg
    99661 caacaatttc agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg
    99721 ccatcccgat gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta
    99781 atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag
    99841 attcattcga aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga
    99901 taccgccaga aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca
    99961 gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg
   100021 tttcccatgg aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa
   100081 gagcaggtgc cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg
   100141 cactggctaa tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata
   100201 cagttggatt tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta
   100261 cctcacgttt taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct
   100321 tcatcttcca tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta
   100381 ttaataaaac aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg
   100441 gattttaagc aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga
   100501 acattcactt tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt
   100561 ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat
   100621 tcttttgtat cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt
   100681 ccccagcctt ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta
   100741 tgttttgctc gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt
   100801 cctccttttt attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg
   100861 gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat
   100921 gttctcgtag atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa
   100981 tacctctggt gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt
   101041 tgagatgctg agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc
   101101 tgcaccaatt ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat
   101161 cacacgtgtt ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct
   101221 ataaactgat tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga
   101281 aaaataaagc aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa
   101341 acggcaatga atgccgctct tcttatttcg attcttttat aattctgctt ctgattcatc
   101401 tttggttagt gtgtgtctaa cactagttac gccaaatgtt tgatcgattt tttccttcaa
   101461 cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca ttgatggatt caatgaccag
   101521 caccgctgct tgtgtttctt ccgtcagcat cgttctttgg gcttcacgcc agttgagaca
   101581 gcgacagatc gctccttgtt catcatagta gatgatttct tccggaagtg ccggagcatc
   101641 ggaatctgca cctaaaggca ggaatgattc gccaccttgg gcttttccta aatgcagatt
   101701 tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg ccaaaagaca aggaaatact
   101761 attatagata tctacaagcg gattgattgg gaaaaattcc cgttcttgat ttgcacgttt
   101821 cagtaaagct tcaatagaag aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg
   101881 acgccactga gcaatgactt cgttttggct gaatgtttct tctgtaaggt aatttcttgc
   101941 ttcttttgct gcctcattca ataattgatg atacgtttct tcactttctt ttgttacgtg
   102001 gttatcgata ccatttacgg ataagatttc gatggttgct tcaggaaata cttcccagaa
   102061 attggtatcc actattactt ttttcatctg ttcacacctc ttgattagaa taattgcttg
   102121 atgaatagat agtctgtttg gggatcatct cccatataaa aaacatgttc gctgtatttg
   102181 ataaatcctt ttttacgata aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc
   102241 caaatggctg ttttttgctt ttcttttgct ttttcaatag ctgtatctag taaaaatgta
   102301 ccgatacctt gatgtttaaa tttttttcgt acatagattc gctcgatttc taaaaaatga
   102361 tcagccattt cctcagattg actcgttcct gtatttagtt tcaggtaacc cgcaggctct
   102421 tgatcgatac tcactaggaa aaaatcggtt tctttatctt ttagctcttc tgtaagtttt
   102481 tctagtgcat aagcttgtgt caagtaatca gctaaatgct ccggtgaatt atcttttcca
   102541 aatgtatctg taaatgtttc gatactaatt tgttgtagta agtctacatc tgaaggcgtc
   102601 atttttcgaa tttcgtattt attcacggct tcttcctttc aatagatcct tttattgcct
   102661 tttttgacaa aattccaatc ttcgctaaca ttcttgcata ctttatttaa aagatattcc
   102721 aattgttttg cttcttcgtc agaaagacca tttaatgcga ctgcattgga gtagttgttt
   102781 tctcgaatga tgaaaggata gatttctgct ccttttttag tcggatagag tttcttgatt
   102841 tttttatttt ctttatcttc caaacgttcg atcatcccat tggattctaa tttcttgatt
   102901 gctcgggcag tcgttgtacg atctactttg atcatttctg ctagtttttc ttggatgatt
   102961 ccaggatttt cgcaaatacg tactaaataa agatactgtc ctcttgttaa atcgacttct
   103021 ttgaattcga tattgctaat agaatctagc gctcgggcaa tcattccgat gtctcgaagg
   103081 atttcagcca tttcatccca cctttatttt gtttgtaaga ctagttcacc tctttttgtt
   103141 gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt ttaataacta gtaaagtata
   103201 ttcttttctt cttttacgaa aaagctcaat cacaatagat ctggtttttg ttttctaaaa
   103261 taagtaactg tttcttcgtt gttcgctttt ctgatagctt tggcaactct tctatactaa
   103321 aaaatcccat gttggaagtt tcattattct caataaactt ggcctcttca ccgtggatcg
   103381 cacaagcaaa aacaagttta taatactgaa aaagctgtgg aatatctttt tgtttattcg
   103441 tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa tcctgtttct tcatagactt
   103501 ctttacgcac attttcttca ggagacaatc ctatttcagc aaatccacca ggaagtgacc
   103561 attctttcgt ccgtttatct tcgattagta ggaccttgcc atcttttttg atataggcac
   103621 gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt
   103681 cgaaagtttc ttttcctatt tcactgatca gttccaatga aatcgtccgg atctcctcaa
   103741 aacgctcttt atcaaataca tcttttccat aatatagacc agcttgagaa agtgcgagta
   103801 gtcttttgta ttgagccaac ttcttgttca ttctcttccc ttctttttta gcaaatgtat
   103861 gactatgtaa tcaagtatag aagactggtt taattacggc aaataaatat aatgaaatct
   103921 gcaataaata aaagaatgtt aaaaaactcg agaacaaaag acattcgact tctctggggc
   103981 tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa
   104041 tatgacgagc tattcttgtc tggctgttct tattaattga ggcaggacag ctttttcaca
   104101 acctccccac taaacggtgt tctcctacca actcctcggt cacaagtcac aatattcatc
   104161 aaacatgaga agctgttttt ttgaaattgc tccttgtgac tcggagctga acgacaggct
   104221 cacgacctct cggtaaacag cgttcaaaag ccaatttttc gatattaagc catttctttg
   104281 ttacttattg tcattgattt ctttttctaa tttatccaat agtggagtgt aatccacttg
   104341 agtatagtct gttacatcaa attcatatag tgttcctaac tggaatttgt catattttcg
   104401 gtcggcgatg atctttttga atgcttcttt cgtaatatga ttggtccctt tcgttcgatc
   104461 ttccaatacg tcaccatagt gataatgatc catgatatag ctcaaatgac gatctgattc
   104521 acgatcacgt ttgtaccgac gttgccataa cacttcaaag tctgctgtta ggcgaatcgt
   104581 gatcacctga tatccgtatc tatctgctaa ttcttggagt cgtgcttttt gcttatcact
   104641 aaaaggatac tcggacacaa tcactttctt tcctgcttcc atatacaaat caagtgcttt
   104701 gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa
   104761 cccgatggat tctgcatagt attccttgat ttcatcgagc gtgatggcat acatatctgg
   104821 aaatcgttcc cgtagtttat tcataagata tgtttttcct gtaccaggac taccagccag
   104881 aagaatgaaa tactttttca tctcatttat ttcctaagtt aattggactt tccaagttca
   104941 taaacagtcc aatcttatgc ttgacaattt cttttactgc ttcatttgct ggaatcacat
   105001 tatggcgaag agacttgttg atttctgttt cactaaaggt ttctttagct actttcgtcc
   105061 agttgactaa aagctccgtt ccaacattga atttgcggat cccgttattg atcaattcag
   105121 gataatcttc ttctttcaca cctgttcctc catggataac tagggggaca tcaactactg
   105181 catcgatttc tttcagcaag ggaatgttta cttctgtttt tgatttgaat tgtccatgat
   105241 tcgtaccgat agcaatagct aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt
   105301 cttccggtct tgtatatacc ttgtcatttt cagcgacatg gattccttct tctgtaccgc
   105361 cgattgttcc aagttctcct tctacggaaa caccgtgcgc atgggcatat tctactactg
   105421 cttttgtctt caggatattt tctttgaaag gcagatggga cccatcatac atgacagaag
   105481 tatatccgct gtcgattgct tctttgatat catcaaagtc tcgggcgtgg tctaagtgca
   105541 agactgcatc aaccatttct tcttctgcaa tagctgtcat tacattgact aacacatgca
   105601 tgccgatata tttagcggta tcgacacttg tttgaataat gatcggcgaa cccatttctt
   105661 ttgctgcgcc tagcatagca ggaagcattt ctaggttatg cgtattaaaa gctccaactg
   105721 taaaattcaa ctcttctgct gtttttgtta cttctttcaa tgttgtatac atcttttatt
   105781 gccaccttat ctttttattt gtttcctttg atcgttactt gttttgcaat ttgtcgcatc
   105841 tcatccattt tgcccatgta gtaatcgatc ccttgatcat caccagcacg agcagcatct
   105901 gcacgcttct ggttatagag gctcatcagt tttttatacc catctccgat cgtctgttta
   105961 ttttccaaac tcttttctaa atattggatg gcttgatcgt tcacatacaa ttcagaaaat
   106021 ttaagtccaa cttgttcata aagtttcgct aatttatgct tatcttgggt agtggaaatc
   106081 tcttgtttca attgtgtaat ctctgattga atcttttcgg tcgcttctgg cgatagggct
   106141 ttttcttctt ttgccagaac ttttttcggt tctttttctt ttttcttaaa aaatccaaac
   106201 atcattttcc ctactttcaa ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat
   106261 aagagtgctg cggctactgt atctacatcg gttgctgtag cattgttgaa tcccatcgaa
   106321 ttaaagatcg ttactagcaa agcgggcaat aatgtaataa agaaaccatg agcaataccg
   106381 ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa atatccctgc tgtccctccg
   106441 gcaaagaaat tggttagcat accaggtaaa atcatcgcta agccgaacat gggcaaagta
   106501 aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg
   106561 ctataaggaa agaaaactgg acaatctaag gcagggatcg catcaggaac taatttcata
   106621 gctatccctc gaaatgccgg cacgatttca cctaacagta gacgtacacc agctagcaat
   106681 acatacacac ccactacgaa ttggatggct tgtaaaaagg caaacatgat atagttttga
   106741 tctcctgata attcagaagc gaatggttcg cctgcaaata aaacagtgat gatatatagc
   106801 gggaccatca cgaccatgac agacaaataa gtatcttgca gaaattcgaa atgtgtcggc
   106861 aacttgatat cttcgatcga ctttttattt tcgcctttct caccaaaaag cttagcaaca
   106921 ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta aagcaatatc atttgatcca
   106981 gtgattttac gaatgatcgg ttgcgctact gccggcatag caatggcgaa acaagctccg
   107041 ataaaaccac cgaccaagat caatacgatc cctcgcagac cggcaaaata gccaaagact
   107101 gtcgtcatcg ttgccatcca taaaattgct tgaccagtta aaaaaatgta cttccatttt
   107161 gtgaaacgag ccaaaataat attgaataca aaaatggcta agaacgtcag tgcaatatct
   107221 ctacctaaac ccaaatcatt cattgcttga ccattgatag attcaataga agggatgatc
   107281 ccttgcatat gaaatccttc agtaaagatt ttcccgaaat aagttaaact tccaacgatg
   107341 atgctcgaac cggcactcaa cacttgaaat cctaggagtg tctttaacgt gccagagatg
   107401 acttgtcctg ctgatttctt ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg
   107461 atcgatgcct gagtcaaaat attttcgatg ataaaattga taacgcccat gtt
//
LOCUS       DACIFS010000002        81218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.002, whole genome shotgun sequence.
ACCESSION   DACIFS010000002 DACIFS010000000
VERSION     DACIFS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.002"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            102..1373
                     /locus_tag="IYA83_000094"
     CDS             102..1373
                     /locus_tag="IYA83_000094"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ8585565.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            1370..2665
                     /locus_tag="IYA83_000095"
     CDS             1370..2665
                     /locus_tag="IYA83_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ8585566.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            2764..3027
                     /locus_tag="IYA83_000096"
     CDS             2764..3027
                     /locus_tag="IYA83_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ8585567.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            3472..3921
                     /locus_tag="IYA83_000097"
     CDS             3472..3921
                     /locus_tag="IYA83_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8585568.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            3978..5396
                     /locus_tag="IYA83_000098"
     CDS             3978..5396
                     /locus_tag="IYA83_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585569.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            5396..6256
                     /locus_tag="IYA83_000099"
     CDS             5396..6256
                     /locus_tag="IYA83_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ8585570.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            6430..7203
                     /locus_tag="IYA83_000100"
     CDS             6430..7203
                     /locus_tag="IYA83_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8585571.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            7350..7715
                     /locus_tag="IYA83_000101"
     CDS             7350..7715
                     /locus_tag="IYA83_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ8585572.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(7863..9071)
                     /locus_tag="IYA83_000102"
     CDS             complement(7863..9071)
                     /locus_tag="IYA83_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8585573.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(9090..9368)
                     /locus_tag="IYA83_000103"
     CDS             complement(9090..9368)
                     /locus_tag="IYA83_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585574.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            9561..10217
                     /locus_tag="IYA83_000104"
     CDS             9561..10217
                     /locus_tag="IYA83_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ8585575.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(10659..11456)
                     /locus_tag="IYA83_000105"
     CDS             complement(10659..11456)
                     /locus_tag="IYA83_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ8585576.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(11453..12637)
                     /locus_tag="IYA83_000106"
     CDS             complement(11453..12637)
                     /locus_tag="IYA83_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ8585577.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(12662..13909)
                     /locus_tag="IYA83_000107"
     CDS             complement(12662..13909)
                     /locus_tag="IYA83_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ8585578.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            14124..15869
                     /gene="dnaX"
                     /locus_tag="IYA83_000108"
     CDS             14124..15869
                     /gene="dnaX"
                     /locus_tag="IYA83_000108"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ8585579.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            15889..16203
                     /locus_tag="IYA83_000109"
     CDS             15889..16203
                     /locus_tag="IYA83_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ8585580.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            16228..16824
                     /gene="recR"
                     /locus_tag="IYA83_000110"
     CDS             16228..16824
                     /gene="recR"
                     /locus_tag="IYA83_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ8585581.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            16888..17532
                     /locus_tag="IYA83_000111"
     CDS             16888..17532
                     /locus_tag="IYA83_000111"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ8585582.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            17547..17876
                     /locus_tag="IYA83_000112"
     CDS             17547..17876
                     /locus_tag="IYA83_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585583.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            17890..18828
                     /gene="holB"
                     /locus_tag="IYA83_000113"
     CDS             17890..18828
                     /gene="holB"
                     /locus_tag="IYA83_000113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ8585584.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            18864..19688
                     /locus_tag="IYA83_000114"
     CDS             18864..19688
                     /locus_tag="IYA83_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ8585585.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            19681..20028
                     /locus_tag="IYA83_000115"
     CDS             19681..20028
                     /locus_tag="IYA83_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ8585586.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            20097..20969
                     /gene="rsmI"
                     /locus_tag="IYA83_000116"
     CDS             20097..20969
                     /gene="rsmI"
                     /locus_tag="IYA83_000116"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ8585587.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            21078..22199
                     /gene="dinB"
                     /locus_tag="IYA83_000117"
     CDS             21078..22199
                     /gene="dinB"
                     /locus_tag="IYA83_000117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ8585588.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(22253..22855)
                     /gene="nrdG"
                     /locus_tag="IYA83_000118"
     CDS             complement(22253..22855)
                     /gene="nrdG"
                     /locus_tag="IYA83_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ8585589.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(23170..25359)
                     /gene="nrdD"
                     /locus_tag="IYA83_000119"
     CDS             complement(23170..25359)
                     /gene="nrdD"
                     /locus_tag="IYA83_000119"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ8585590.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            25685..26452
                     /locus_tag="IYA83_000120"
     CDS             25685..26452
                     /locus_tag="IYA83_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585591.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            26442..28484
                     /locus_tag="IYA83_000121"
     CDS             26442..28484
                     /locus_tag="IYA83_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585592.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            28765..28914
                     /locus_tag="IYA83_000122"
     CDS             28765..28914
                     /locus_tag="IYA83_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ8585593.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            28925..30439
                     /gene="dltA"
                     /locus_tag="IYA83_000123"
     CDS             28925..30439
                     /gene="dltA"
                     /locus_tag="IYA83_000123"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ8585594.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            30436..31641
                     /gene="dltB"
                     /locus_tag="IYA83_000124"
     CDS             30436..31641
                     /gene="dltB"
                     /locus_tag="IYA83_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ8585595.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            31686..31919
                     /gene="dltC"
                     /locus_tag="IYA83_000125"
     CDS             31686..31919
                     /gene="dltC"
                     /locus_tag="IYA83_000125"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ8585596.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            31922..33190
                     /gene="dltD"
                     /locus_tag="IYA83_000126"
     CDS             31922..33190
                     /gene="dltD"
                     /locus_tag="IYA83_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ8585597.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            33331..34413
                     /locus_tag="IYA83_000127"
     CDS             33331..34413
                     /locus_tag="IYA83_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ8585598.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            34413..35216
                     /locus_tag="IYA83_000128"
     CDS             34413..35216
                     /locus_tag="IYA83_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ8585599.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            35286..36026
                     /locus_tag="IYA83_000129"
     CDS             35286..36026
                     /locus_tag="IYA83_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ8585600.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            36037..37041
                     /locus_tag="IYA83_000130"
     CDS             36037..37041
                     /locus_tag="IYA83_000130"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ8585601.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(37086..38930)
                     /locus_tag="IYA83_000131"
     CDS             complement(37086..38930)
                     /locus_tag="IYA83_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585602.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            39142..39660
                     /locus_tag="IYA83_000132"
     CDS             39142..39660
                     /locus_tag="IYA83_000132"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ8585603.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            39788..40471
                     /locus_tag="IYA83_000133"
     CDS             39788..40471
                     /locus_tag="IYA83_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ8585604.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            40780..40853
                     /locus_tag="IYA83_000134"
     tRNA            40780..40853
                     /locus_tag="IYA83_000134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40814..40816,aa:Arg,seq:tct)
     gene            41012..41371
                     /locus_tag="IYA83_000135"
     CDS             41012..41371
                     /locus_tag="IYA83_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ8585605.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            41494..43125
                     /locus_tag="IYA83_000136"
     CDS             41494..43125
                     /locus_tag="IYA83_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ8585606.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(43187..43828)
                     /locus_tag="IYA83_000137"
     CDS             complement(43187..43828)
                     /locus_tag="IYA83_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ8585607.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            44110..44418
                     /gene="rplU"
                     /locus_tag="IYA83_000138"
     CDS             44110..44418
                     /gene="rplU"
                     /locus_tag="IYA83_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ8585608.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            44445..44789
                     /locus_tag="IYA83_000139"
     CDS             44445..44789
                     /locus_tag="IYA83_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ8585609.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            44802..45095
                     /gene="rpmA"
                     /locus_tag="IYA83_000140"
     CDS             44802..45095
                     /gene="rpmA"
                     /locus_tag="IYA83_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ8585610.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            45502..48336
                     /locus_tag="IYA83_000141"
     CDS             45502..48336
                     /locus_tag="IYA83_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8585611.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            48527..49018
                     /locus_tag="IYA83_000142"
     CDS             48527..49018
                     /locus_tag="IYA83_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ8585612.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            49121..50110
                     /gene="manX"
                     /locus_tag="IYA83_000143"
     CDS             49121..50110
                     /gene="manX"
                     /locus_tag="IYA83_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ8585613.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            50143..50946
                     /locus_tag="IYA83_000144"
     CDS             50143..50946
                     /locus_tag="IYA83_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ8585614.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            50967..51881
                     /locus_tag="IYA83_000145"
     CDS             50967..51881
                     /locus_tag="IYA83_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ8585615.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            51964..52338
                     /locus_tag="IYA83_000146"
     CDS             51964..52338
                     /locus_tag="IYA83_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ8585616.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            52477..52722
                     /locus_tag="IYA83_000147"
     CDS             52477..52722
                     /locus_tag="IYA83_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ8585617.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            52894..53958
                     /locus_tag="IYA83_000148"
     CDS             52894..53958
                     /locus_tag="IYA83_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ8585618.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            54204..54629
                     /locus_tag="IYA83_000149"
     CDS             54204..54629
                     /locus_tag="IYA83_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ8585619.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            54622..55071
                     /gene="nusB"
                     /locus_tag="IYA83_000150"
     CDS             54622..55071
                     /gene="nusB"
                     /locus_tag="IYA83_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ8585620.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            55168..56022
                     /locus_tag="IYA83_000151"
     CDS             55168..56022
                     /locus_tag="IYA83_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ8585621.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            56023..57360
                     /locus_tag="IYA83_000152"
     CDS             56023..57360
                     /locus_tag="IYA83_000152"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ8585622.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            57365..57589
                     /locus_tag="IYA83_000153"
     CDS             57365..57589
                     /locus_tag="IYA83_000153"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ8585623.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            57591..58481
                     /locus_tag="IYA83_000154"
     CDS             57591..58481
                     /locus_tag="IYA83_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ8585624.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLTRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            58490..59308
                     /locus_tag="IYA83_000155"
     CDS             58490..59308
                     /locus_tag="IYA83_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ8585625.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            59415..59864
                     /locus_tag="IYA83_000156"
     CDS             59415..59864
                     /locus_tag="IYA83_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ8585626.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            59882..61564
                     /gene="recN"
                     /locus_tag="IYA83_000157"
     CDS             59882..61564
                     /gene="recN"
                     /locus_tag="IYA83_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ8585627.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(61619..61735)
                     /locus_tag="IYA83_000158"
     CDS             complement(61619..61735)
                     /locus_tag="IYA83_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ8585628.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(61808..62764)
                     /locus_tag="IYA83_000159"
     CDS             complement(61808..62764)
                     /locus_tag="IYA83_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ8585629.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            62935..63303
                     /locus_tag="IYA83_000160"
     CDS             62935..63303
                     /locus_tag="IYA83_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ8585630.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            63473..63904
                     /gene="mraZ"
                     /locus_tag="IYA83_000161"
     CDS             63473..63904
                     /gene="mraZ"
                     /locus_tag="IYA83_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ8585631.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            63925..64884
                     /gene="rsmH"
                     /locus_tag="IYA83_000162"
     CDS             63925..64884
                     /gene="rsmH"
                     /locus_tag="IYA83_000162"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ8585632.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            64889..65290
                     /gene="ftsL"
                     /locus_tag="IYA83_000163"
     CDS             64889..65290
                     /gene="ftsL"
                     /locus_tag="IYA83_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ8585633.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            65291..67483
                     /locus_tag="IYA83_000164"
     CDS             65291..67483
                     /locus_tag="IYA83_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ8585634.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            67509..68471
                     /locus_tag="IYA83_000165"
     CDS             67509..68471
                     /locus_tag="IYA83_000165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ8585635.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            68485..69846
                     /locus_tag="IYA83_000166"
     CDS             68485..69846
                     /locus_tag="IYA83_000166"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ8585636.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            69872..70960
                     /gene="murG"
                     /locus_tag="IYA83_000167"
     CDS             69872..70960
                     /gene="murG"
                     /locus_tag="IYA83_000167"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ8585637.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            70975..72195
                     /locus_tag="IYA83_000168"
     CDS             70975..72195
                     /locus_tag="IYA83_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ8585638.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            72351..73676
                     /gene="ftsA"
                     /locus_tag="IYA83_000169"
     CDS             72351..73676
                     /gene="ftsA"
                     /locus_tag="IYA83_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ8585639.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            73699..74940
                     /gene="ftsZ"
                     /locus_tag="IYA83_000170"
     CDS             73699..74940
                     /gene="ftsZ"
                     /locus_tag="IYA83_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ8585640.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            74957..75634
                     /locus_tag="IYA83_000171"
     CDS             74957..75634
                     /locus_tag="IYA83_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ8585641.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            75648..76247
                     /locus_tag="IYA83_000172"
     CDS             75648..76247
                     /locus_tag="IYA83_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ8585642.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            76262..76549
                     /locus_tag="IYA83_000173"
     CDS             76262..76549
                     /locus_tag="IYA83_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ8585643.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            76598..77380
                     /locus_tag="IYA83_000174"
     CDS             76598..77380
                     /locus_tag="IYA83_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ8585644.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            77401..78108
                     /locus_tag="IYA83_000175"
     CDS             77401..78108
                     /locus_tag="IYA83_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ8585645.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            78370..81168
                     /gene="ileS"
                     /locus_tag="IYA83_000176"
     CDS             78370..81168
                     /gene="ileS"
                     /locus_tag="IYA83_000176"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ8585646.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
BASE COUNT        26608 a        13899 c        17301 g        23410 t
ORIGIN      
        1 ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga taaaatacaa
       61 gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa atcgtaatca
      121 aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat agtgcagtcg
      181 cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt gtgccggaca
      241 tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact gtccattttt
      301 ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg ccgcacggaa
      361 aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt aaatttggcg
      421 aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt gacctacacg
      481 tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc atgtatttac
      541 ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt tcagtcggag
      601 caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta attgaaaacg
      661 ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg ggggcaaaag
      721 ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta catggctgtc
      781 ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta gcagctgcag
      841 taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc tttatagcaa
      901 aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa gtttacccct
      961 cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt gccacagatc
     1021 tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa atcatcgata
     1081 cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc gctgatatta
     1141 ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc acagaagtcg
     1201 tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg gcagaaggga
     1261 caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt atcgagaaac
     1321 taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa tgagcgatta
     1381 tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt atgccaaaga
     1441 atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag ctgtgattcg
     1501 tgcacaagca gagaaacaag gattttatta tatggaagga atattggata tcgttggtca
     1561 agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag atatctatat
     1621 ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg caggaaaagc
     1681 ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa gcgttaatgg
     1741 aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac ctctttatcc
     1801 gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa tgatcgatat
     1861 cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa aagcagggaa
     1921 aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg aagtagaatt
     1981 aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac gaagcgtgaa
     2041 aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc gagtttctga
     2101 attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg ttatcttgat
     2161 ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa gcggacgtac
     2221 attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt tcggggcagc
     2281 aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag tcgatacagg
     2341 aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc tagaattaca
     2401 actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca aaaaatcagg
     2461 tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt ggaaacttcg
     2521 aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct ttttgcgtaa
     2581 aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg gtaaaaaaaa
     2641 tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta agatgttaat
     2701 gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag gagcgaaaaa
     2761 atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga ttcaacaact
     2821 ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga atgggaagac
     2881 ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc attctacact
     2941 ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa aaaatacggt
     3001 ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt ttagcagact
     3061 atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt cgtttgatgc
     3121 tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat agttttaatt
     3181 atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt cgaagtaaaa
     3241 aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt ttgttttgtt
     3301 atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata cacgcggatg
     3361 atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg atgctatact
     3421 gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt tatgaggttt
     3481 gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc aaaaggatca
     3541 ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa tgttcaaaaa
     3601 gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga agatggattt
     3661 gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt ttttcataca
     3721 gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat ttttgccatc
     3781 gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact ctccagacga
     3841 ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc agatatcatc
     3901 agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga tagtgaagaa
     3961 aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact ggaattgctc
     4021 atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac catcaagcaa
     4081 aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa atcaaagagg
     4141 cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt gccgggaaaa
     4201 aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta atagacgaag
     4261 cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct gcaaatgaga
     4321 aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt ccatttgtga
     4381 tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg gctgatccca
     4441 actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga gatacagcgt
     4501 ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct ggaaaagcag
     4561 gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca ggttttcttg
     4621 gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca aataaaatta
     4681 gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt ttatcgatct
     4741 taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg gtgttggatg
     4801 ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc ggattactta
     4861 tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact tcgactttct
     4921 cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg gctgcaggaa
     4981 tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa tttaccaaag
     5041 aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt acagaaggtg
     5101 ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg acaggttcag
     5161 cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct catggaggga
     5221 tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc ttggcaattg
     5281 catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca gtgaaagaga
     5341 aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa gcgtaatgtt
     5401 atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag taccagcctt
     5461 caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga gattagaagc
     5521 accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg agtttgttgc
     5581 ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact tggatcatgg
     5641 cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg tgatgatcga
     5701 tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg ttgcgttagc
     5761 tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata atggttctgc
     5821 tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga tttttgtgaa
     5881 agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct tgtatccgaa
     5941 aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac aaatcgatct
     6001 tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg aagcagtaaa
     6061 atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt tcaatgcttt
     6121 gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt atccagcagc
     6181 taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca cagtaggcaa
     6241 ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat agtcttgtca
     6301 taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt cttcttattt
     6361 ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata aagtaaatgg
     6421 ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa ttatctaaaa
     6481 aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag cgaagccacg
     6541 attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt tcgcggtggc
     6601 gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat caccaaatta
     6661 gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat tcgagatgat
     6721 atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa cctagcaaga
     6781 tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt gagtgaatat
     6841 acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc aacaatcaac
     6901 ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat aatgagttgt
     6961 cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc aatggtcaaa
     7021 aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca aaaaattgca
     7081 cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt gacaagtaaa
     7141 gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg tctgtacgga
     7201 taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac cttatctttg
     7261 ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga taatcttact
     7321 tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg
     7381 caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt gttagcttta
     7441 tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta ggggatctgt
     7501 tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt tcagctgcag
     7561 ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg atgaaagtat
     7621 tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc
     7681 aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact attcttatcg
     7741 ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata aactattttt
     7801 actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc tattcatcaa
     7861 gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg actatgtttt
     7921 cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc aatcacatac
     7981 aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc aacgcctaag
     8041 cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg ttcgcggacg
     8101 atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag gccgactaat
     8161 gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa taatcctgct
     8221 gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc gaaaatcctt
     8281 ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa aaatgaccca
     8341 gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac acttgaataa
     8401 gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc aataaaacta
     8461 ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat attttttaca
     8521 ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa ccagataatg
     8581 aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac agcggcagct
     8641 aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc agggatatat
     8701 gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc attcaagaat
     8761 cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt tgaaaggaga
     8821 tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa ctgtttcccc
     8881 atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa acctgcttgg
     8941 ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat caacagataa
     9001 taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt attaaataat
     9061 ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa tttttgtatt
     9121 tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg aataaataaa
     9181 tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg attctcatcc
     9241 actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac taaagaagct
     9301 ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt gttcatccag
     9361 taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat aacgaataaa
     9421 aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt taatatcatt
     9481 agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt ttagatgaaa
     9541 gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg gataaaaatg
     9601 attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg atggaagatt
     9661 cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca aatgatggga
     9721 gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta aaagacgaat
     9781 attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat gcagagtttt
     9841 atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag ctacaacact
     9901 atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc
     9961 tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat gatttattcg
    10021 gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa gaaatcgcca
    10081 aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag
    10141 atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac ttgttagaag
    10201 acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac ctgagtatta
    10261 gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    10321 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tctgagtcac
    10381 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag
    10441 gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct gcatcaagta
    10501 ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga cttatcaccg
    10561 tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac acgatttttg
    10621 tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg
    10681 ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt
    10741 gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca
    10801 gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga
    10861 taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca
    10921 atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct
    10981 acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa
    11041 gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc
    11101 gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt
    11161 tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct
    11221 cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc
    11281 aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt
    11341 tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc
    11401 accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca
    11461 agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc
    11521 ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg
    11581 cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca
    11641 gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg
    11701 ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt
    11761 gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta
    11821 aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca
    11881 tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc
    11941 ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata
    12001 tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat
    12061 aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa
    12121 caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag
    12181 caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg
    12241 gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac
    12301 cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg
    12361 tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa
    12421 gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt
    12481 cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat
    12541 agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac
    12601 cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa
    12661 attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc
    12721 cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa
    12781 cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc
    12841 cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt
    12901 ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat
    12961 ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc
    13021 cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt acgctaggat
    13081 cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag
    13141 aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat
    13201 ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc
    13261 gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta
    13321 aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg
    13381 tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata
    13441 attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc
    13501 cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa
    13561 atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag
    13621 gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc
    13681 caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta
    13741 aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac
    13801 cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat
    13861 ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct
    13921 cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg
    13981 aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga
    14041 aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa
    14101 ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc
    14161 agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg
    14221 tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga
    14281 gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat
    14341 gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa
    14401 tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt
    14461 atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa
    14521 caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca
    14581 tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat
    14641 ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag
    14701 aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg
    14761 ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta
    14821 ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact
    14881 tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg
    14941 cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc
    15001 tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat
    15061 ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga
    15121 gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta
    15181 cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag
    15241 atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg
    15301 aactacgcag taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc
    15361 ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg
    15421 aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc
    15481 tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag
    15541 gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat
    15601 tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg
    15661 tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag
    15721 aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc
    15781 ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct
    15841 ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga
    15901 atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa
    15961 gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa
    16021 gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat
    16081 cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca
    16141 caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt
    16201 tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc
    16261 gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat
    16321 acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa
    16381 cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata
    16441 tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta
    16501 atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta
    16561 tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg
    16621 catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca
    16681 acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca
    16741 catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct
    16801 gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa
    16861 agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct
    16921 gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca
    16981 aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga
    17041 gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat
    17101 gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa
    17161 atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc
    17221 atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat
    17281 tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga
    17341 caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat
    17401 gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa
    17461 agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt
    17521 tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag
    17581 acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat
    17641 cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc
    17701 gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat
    17761 ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag
    17821 aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ttttaggagg
    17881 agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag
    17941 cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa
    18001 ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg
    18061 aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat
    18121 ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt
    18181 ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa
    18241 gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc
    18301 cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt
    18361 caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga
    18421 ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt
    18481 ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt
    18541 tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa
    18601 aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg
    18661 ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa
    18721 cagataaacc agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc
    18781 agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag
    18841 gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag
    18901 gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag
    18961 tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc
    19021 ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag
    19081 aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag
    19141 tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata
    19201 aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc
    19261 tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa
    19321 agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg
    19381 atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa
    19441 aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg
    19501 agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag
    19561 ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg
    19621 aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga
    19681 atggataaga gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca
    19741 ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact
    19801 ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa
    19861 gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt
    19921 tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag
    19981 ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga
    20041 ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt
    20101 ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc
    20161 caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc
    20221 ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata
    20281 caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt
    20341 tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg
    20401 atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc
    20461 caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt
    20521 tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa
    20581 aagaacagac agttacgctg attttttatg agtcacctta tcggatcact gctacattga
    20641 cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg
    20701 tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc
    20761 cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg
    20821 aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg
    20881 aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag
    20941 tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta
    21001 atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata
    21061 atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa
    21121 aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg
    21181 agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag
    21241 tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac
    21301 aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca
    21361 gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag
    21421 ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca
    21481 tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg
    21541 cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag
    21601 gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa
    21661 aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag
    21721 gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct
    21781 attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc
    21841 gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc
    21901 tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac
    21961 atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag
    22021 tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat
    22081 gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc
    22141 tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa
    22201 aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca
    22261 agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca
    22321 caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc
    22381 gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga
    22441 tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc
    22501 ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt
    22561 gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt
    22621 aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat
    22681 atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa
    22741 cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa
    22801 tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa
    22861 aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca
    22921 taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag
    22981 agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc
    23041 tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac
    23101 gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt
    23161 ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac
    23221 catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt
    23281 ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc
    23341 accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat
    23401 tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg
    23461 gtattcacag tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc
    23521 caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata
    23581 ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg
    23641 gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc
    23701 ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc
    23761 tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta
    23821 gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga
    23881 aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc
    23941 tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag
    24001 taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa
    24061 tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca
    24121 cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat
    24181 tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg
    24241 tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat
    24301 cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa
    24361 aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt
    24421 gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat
    24481 agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga
    24541 atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc
    24601 aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc
    24661 aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc
    24721 attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt
    24781 catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc
    24841 atttgctata tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt
    24901 cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt
    24961 ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc
    25021 aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc
    25081 tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg
    25141 gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg
    25201 tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc
    25261 atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg
    25321 ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat
    25381 tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata
    25441 gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga
    25501 aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata
    25561 aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa
    25621 attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga
    25681 agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc
    25741 tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat
    25801 tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt
    25861 gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga
    25921 gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga
    25981 taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga
    26041 ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa
    26101 acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact
    26161 tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc
    26221 ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact
    26281 gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga
    26341 tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt
    26401 aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg
    26461 ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat
    26521 ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag
    26581 caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg
    26641 ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa
    26701 taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag
    26761 aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt attatttttt
    26821 ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt
    26881 ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag
    26941 tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa
    27001 aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca
    27061 tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt
    27121 catggatgat cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt
    27181 ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga
    27241 tcatattgag taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca
    27301 cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa
    27361 tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga
    27421 aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga
    27481 ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg
    27541 ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg
    27601 aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat
    27661 cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat
    27721 atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa
    27781 gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg
    27841 tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa
    27901 ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg
    27961 tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac
    28021 ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct
    28081 cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca
    28141 cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat
    28201 tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac
    28261 aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga
    28321 tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg
    28381 ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt
    28441 atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca
    28501 agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg
    28561 atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa
    28621 aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa
    28681 aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt
    28741 gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat
    28801 tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat
    28861 ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa
    28921 gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga
    28981 tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga
    29041 cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg
    29101 caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta
    29161 tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa
    29221 accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat
    29281 aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag
    29341 cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa
    29401 aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt
    29461 tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt
    29521 attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt
    29581 tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt
    29641 ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt
    29701 gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag
    29761 tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac
    29821 tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc
    29881 gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc
    29941 aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa
    30001 caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac
    30061 aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca
    30121 gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt atctgctcca
    30181 agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca
    30241 gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa
    30301 atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca
    30361 ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc
    30421 tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt
    30481 tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga
    30541 ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat
    30601 tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg
    30661 ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt
    30721 ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca
    30781 tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg
    30841 gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg
    30901 ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag
    30961 ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt
    31021 tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct
    31081 gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg
    31141 tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt
    31201 acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg
    31261 aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat
    31321 gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta
    31381 atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg
    31441 tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg
    31501 atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta
    31561 gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta
    31621 gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa
    31681 ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa
    31741 gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc
    31801 aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc
    31861 gagaggattg gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag
    31921 tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt
    31981 tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc
    32041 ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc
    32101 tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca
    32161 tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc
    32221 cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa
    32281 caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga
    32341 ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa
    32401 agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga
    32461 gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga
    32521 atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag
    32581 caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa
    32641 acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac
    32701 gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc
    32761 agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt
    32821 tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc
    32881 ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc
    32941 caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa
    33001 tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt
    33061 ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta
    33121 tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca
    33181 attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct
    33241 gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca
    33301 caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt
    33361 gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga
    33421 gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc
    33481 aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt
    33541 tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg
    33601 tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc
    33661 cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct
    33721 tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag
    33781 tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc
    33841 agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga
    33901 gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg
    33961 caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt
    34021 gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca
    34081 gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat
    34141 aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat
    34201 acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt
    34261 ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat
    34321 gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt
    34381 gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat
    34441 atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt
    34501 tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc
    34561 cggaagaaag catgcagcag cacttattga gttttctagt cgataattta cttttattcg
    34621 cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa
    34681 gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt
    34741 tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa
    34801 tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg
    34861 tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg
    34921 tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt
    34981 tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag
    35041 atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag
    35101 caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag
    35161 aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg
    35221 taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat
    35281 gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga
    35341 caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta
    35401 cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc
    35461 attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc
    35521 ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact
    35581 ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat
    35641 taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg
    35701 ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt
    35761 atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc
    35821 tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg
    35881 cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag
    35941 aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg
    36001 cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac
    36061 cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat
    36121 gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat
    36181 acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc
    36241 ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg
    36301 cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca
    36361 cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat
    36421 ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga
    36481 acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac
    36541 aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt
    36601 cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa
    36661 attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg
    36721 aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag
    36781 tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat
    36841 ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta
    36901 ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta
    36961 tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa
    37021 ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct
    37081 attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa
    37141 gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt
    37201 aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta
    37261 tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac
    37321 aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct
    37381 aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga
    37441 tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag
    37501 ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct
    37561 tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc
    37621 agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt
    37681 cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt
    37741 ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt
    37801 agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc
    37861 gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct
    37921 acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg
    37981 cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa
    38041 gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac
    38101 cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag
    38161 ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa
    38221 atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt
    38281 gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata
    38341 gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa
    38401 ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga
    38461 taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc
    38521 acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc
    38581 ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg
    38641 tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga
    38701 atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc
    38761 cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag
    38821 aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata
    38881 cgtaaataaa aagattcttt tttattatat atgttattaa aaaaagacat aaaggtattc
    38941 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
    39001 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
    39061 atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa
    39121 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
    39181 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
    39241 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
    39301 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
    39361 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
    39421 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
    39481 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
    39541 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
    39601 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
    39661 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
    39721 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
    39781 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
    39841 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
    39901 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
    39961 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
    40021 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
    40081 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
    40141 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
    40201 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
    40261 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
    40321 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
    40381 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
    40441 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
    40501 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
    40561 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
    40621 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
    40681 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
    40741 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
    40801 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
    40861 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
    40921 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
    40981 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
    41041 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
    41101 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
    41161 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
    41221 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
    41281 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
    41341 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
    41401 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
    41461 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
    41521 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
    41581 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
    41641 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
    41701 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
    41761 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
    41821 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
    41881 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
    41941 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
    42001 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
    42061 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
    42121 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
    42181 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
    42241 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
    42301 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
    42361 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
    42421 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
    42481 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
    42541 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
    42601 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
    42661 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
    42721 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
    42781 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
    42841 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
    42901 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
    42961 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
    43021 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
    43081 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
    43141 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
    43201 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
    43261 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
    43321 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
    43381 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
    43441 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
    43501 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
    43561 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
    43621 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
    43681 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
    43741 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
    43801 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
    43861 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
    43921 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
    43981 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
    44041 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
    44101 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
    44161 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
    44221 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
    44281 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
    44341 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
    44401 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
    44461 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
    44521 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
    44581 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
    44641 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
    44701 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
    44761 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
    44821 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
    44881 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
    44941 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
    45001 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
    45061 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
    45121 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
    45181 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
    45241 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
    45301 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
    45361 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
    45421 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
    45481 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
    45541 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
    45601 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
    45661 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
    45721 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
    45781 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
    45841 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
    45901 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
    45961 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
    46021 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
    46081 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
    46141 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
    46201 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
    46261 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
    46321 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
    46381 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
    46441 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
    46501 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
    46561 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
    46621 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
    46681 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
    46741 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
    46801 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
    46861 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
    46921 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
    46981 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
    47041 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
    47101 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
    47161 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
    47221 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
    47281 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
    47341 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
    47401 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
    47461 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
    47521 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
    47581 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
    47641 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
    47701 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
    47761 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
    47821 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
    47881 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
    47941 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
    48001 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
    48061 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
    48121 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
    48181 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
    48241 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
    48301 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
    48361 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
    48421 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
    48481 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
    48541 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
    48601 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
    48661 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
    48721 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
    48781 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
    48841 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
    48901 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
    48961 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
    49021 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
    49081 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    49141 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    49201 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    49261 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    49321 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    49381 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    49441 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    49501 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    49561 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    49621 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    49681 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    49741 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    49801 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    49861 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    49921 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    49981 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    50041 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    50101 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    50161 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    50221 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    50281 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    50341 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    50401 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    50461 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    50521 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    50581 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    50641 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    50701 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    50761 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    50821 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    50881 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    50941 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    51001 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    51061 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    51121 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    51181 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    51241 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    51301 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    51361 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    51421 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    51481 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    51541 tcgtattagc tgcccttgta gaaagatggg tatcaatcaa attcttacca gttgtatcaa
    51601 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    51661 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    51721 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    51781 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    51841 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    51901 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    51961 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    52021 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    52081 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    52141 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    52201 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    52261 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    52321 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    52381 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    52441 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    52501 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    52561 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    52621 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    52681 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    52741 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    52801 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    52861 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    52921 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    52981 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    53041 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    53101 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    53161 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    53221 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    53281 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    53341 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    53401 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    53461 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    53521 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    53581 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    53641 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    53701 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    53761 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    53821 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    53881 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    53941 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    54001 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    54061 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    54121 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    54181 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    54241 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    54301 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    54361 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    54421 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    54481 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    54541 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    54601 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    54661 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    54721 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    54781 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    54841 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    54901 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    54961 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    55021 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    55081 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    55141 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    55201 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    55261 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    55321 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    55381 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    55441 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    55501 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    55561 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    55621 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    55681 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    55741 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    55801 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    55861 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    55921 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    55981 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    56041 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    56101 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    56161 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    56221 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    56281 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    56341 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    56401 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    56461 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    56521 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    56581 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    56641 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    56701 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    56761 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    56821 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    56881 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    56941 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    57001 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    57061 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    57121 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    57181 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    57241 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    57301 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    57361 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    57421 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    57481 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    57541 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    57601 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    57661 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    57721 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    57781 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    57841 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    57901 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    57961 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    58021 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    58081 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgactc
    58141 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    58201 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    58261 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    58321 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    58381 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    58441 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    58501 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    58561 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    58621 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    58681 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    58741 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    58801 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    58861 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    58921 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    58981 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    59041 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    59101 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    59161 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    59221 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    59281 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    59341 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    59401 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    59461 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    59521 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    59581 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    59641 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    59701 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    59761 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    59821 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    59881 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    59941 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    60001 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    60061 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    60121 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    60181 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    60241 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    60301 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    60361 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    60421 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    60481 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    60541 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    60601 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    60661 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    60721 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    60781 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    60841 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    60901 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    60961 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    61021 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    61081 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    61141 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    61201 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    61261 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    61321 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    61381 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    61441 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    61501 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    61561 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    61621 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    61681 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    61741 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    61801 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    61861 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    61921 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    61981 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    62041 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    62101 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    62161 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    62221 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    62281 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    62341 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    62401 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    62461 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    62521 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    62581 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    62641 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    62701 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    62761 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    62821 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    62881 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    62941 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    63001 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    63061 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    63121 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    63181 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    63241 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    63301 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    63361 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    63421 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    63481 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    63541 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    63601 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    63661 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    63721 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    63781 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    63841 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    63901 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    63961 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    64021 ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat
    64081 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    64141 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    64201 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    64261 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    64321 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    64381 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    64441 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    64501 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    64561 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    64621 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    64681 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    64741 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    64801 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    64861 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    64921 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    64981 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    65041 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    65101 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    65161 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    65221 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    65281 agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa
    65341 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggaatgtt
    65401 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    65461 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    65521 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    65581 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    65641 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    65701 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    65761 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    65821 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    65881 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    65941 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    66001 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    66061 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    66121 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    66181 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    66241 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    66301 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    66361 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    66421 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    66481 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    66541 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    66601 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    66661 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    66721 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    66781 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    66841 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaac
    66901 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    66961 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    67021 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    67081 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    67141 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    67201 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    67261 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    67321 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    67381 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    67441 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    67501 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    67561 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    67621 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    67681 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    67741 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    67801 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    67861 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    67921 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    67981 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    68041 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    68101 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    68161 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    68221 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    68281 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    68341 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    68401 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    68461 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    68521 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    68581 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    68641 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    68701 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    68761 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    68821 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    68881 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    68941 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    69001 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    69061 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    69121 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    69181 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    69241 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    69301 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    69361 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    69421 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    69481 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    69541 gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    69601 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    69661 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    69721 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    69781 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    69841 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    69901 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    69961 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    70021 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    70081 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    70141 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    70201 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    70261 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    70321 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    70381 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    70441 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    70501 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    70561 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    70621 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    70681 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    70741 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    70801 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    70861 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    70921 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    70981 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    71041 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    71101 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    71161 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    71221 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    71281 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    71341 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    71401 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    71461 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    71521 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    71581 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    71641 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    71701 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    71761 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    71821 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    71881 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    71941 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    72001 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    72061 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    72121 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    72181 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    72241 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    72301 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    72361 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    72421 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    72481 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    72541 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    72601 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    72661 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    72721 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    72781 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    72841 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    72901 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    72961 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    73021 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    73081 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    73141 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    73201 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    73261 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    73321 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    73381 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    73441 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    73501 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    73561 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    73621 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    73681 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    73741 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    73801 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    73861 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    73921 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    73981 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    74041 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    74101 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    74161 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    74221 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    74281 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    74341 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    74401 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    74461 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    74521 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    74581 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    74641 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    74701 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    74761 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    74821 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    74881 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    74941 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    75001 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    75061 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    75121 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    75181 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    75241 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    75301 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    75361 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    75421 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    75481 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    75541 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    75601 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    75661 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    75721 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    75781 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    75841 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    75901 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    75961 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    76021 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    76081 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    76141 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    76201 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    76261 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    76321 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    76381 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    76441 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    76501 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    76561 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    76621 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    76681 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    76741 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    76801 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    76861 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    76921 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    76981 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    77041 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    77101 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    77161 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    77221 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    77281 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    77341 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    77401 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    77461 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    77521 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    77581 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    77641 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    77701 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    77761 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    77821 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    77881 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    77941 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    78001 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    78061 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    78121 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    78181 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    78241 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    78301 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    78361 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    78421 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    78481 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    78541 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    78601 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    78661 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    78721 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    78781 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    78841 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac
    78901 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    78961 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    79021 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    79081 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    79141 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    79201 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    79261 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    79321 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    79381 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    79441 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    79501 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    79561 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    79621 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    79681 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    79741 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    79801 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    79861 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    79921 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    79981 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    80041 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    80101 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    80161 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    80221 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    80281 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    80341 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    80401 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    80461 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    80521 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    80581 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    80641 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    80701 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    80761 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    80821 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    80881 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    80941 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    81001 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    81061 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    81121 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    81181 ggtgtgacat gttctttgtc acaccccctt ttgttatc
//
LOCUS       DACIFS010000003        79173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.003, whole genome shotgun sequence.
ACCESSION   DACIFS010000003 DACIFS010000000
VERSION     DACIFS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.003"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(38..316)
                     /locus_tag="IYA83_000177"
                     /pseudo
     CDS             complement(38..316)
                     /locus_tag="IYA83_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(519..998)
                     /gene="rlmH"
                     /locus_tag="IYA83_000178"
     CDS             complement(519..998)
                     /gene="rlmH"
                     /locus_tag="IYA83_000178"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ8585647.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1426..2715
                     /locus_tag="IYA83_000179"
     CDS             1426..2715
                     /locus_tag="IYA83_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ8585648.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2754..3359)
                     /locus_tag="IYA83_000180"
     CDS             complement(2754..3359)
                     /locus_tag="IYA83_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ8585649.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3555..4019)
                     /locus_tag="IYA83_000181"
     CDS             complement(3555..4019)
                     /locus_tag="IYA83_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ8585650.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4041..4361)
                     /locus_tag="IYA83_000182"
     CDS             complement(4041..4361)
                     /locus_tag="IYA83_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ8585651.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="IYA83_000183"
     CDS             complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="IYA83_000183"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ8585652.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5783..6133
                     /locus_tag="IYA83_000184"
     CDS             5783..6133
                     /locus_tag="IYA83_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8585653.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            6318..7271
                     /locus_tag="IYA83_000185"
     CDS             6318..7271
                     /locus_tag="IYA83_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8585654.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            7284..9071
                     /locus_tag="IYA83_000186"
     CDS             7284..9071
                     /locus_tag="IYA83_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585655.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            9058..10260
                     /locus_tag="IYA83_000187"
     CDS             9058..10260
                     /locus_tag="IYA83_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585656.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="IYA83_000188"
     CDS             complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="IYA83_000188"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ8585657.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11903..12748
                     /locus_tag="IYA83_000189"
     CDS             11903..12748
                     /locus_tag="IYA83_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ8585658.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12752..13192
                     /locus_tag="IYA83_000190"
     CDS             12752..13192
                     /locus_tag="IYA83_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ8585659.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            13242..13436
                     /locus_tag="IYA83_000191"
     CDS             13242..13436
                     /locus_tag="IYA83_000191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585660.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13572..14258
                     /locus_tag="IYA83_000192"
     CDS             13572..14258
                     /locus_tag="IYA83_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8585661.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14666..15355)
                     /locus_tag="IYA83_000193"
     CDS             complement(14666..15355)
                     /locus_tag="IYA83_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585662.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15330..17459)
                     /locus_tag="IYA83_000194"
     CDS             complement(15330..17459)
                     /locus_tag="IYA83_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ8585663.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17507..18844)
                     /locus_tag="IYA83_000195"
     CDS             complement(17507..18844)
                     /locus_tag="IYA83_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ8585664.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFCGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="IYA83_000196"
     CDS             complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="IYA83_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ8585665.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="IYA83_000197"
     CDS             complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="IYA83_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ8585666.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19780..20304)
                     /gene="infC"
                     /locus_tag="IYA83_000198"
     CDS             complement(19780..20304)
                     /gene="infC"
                     /locus_tag="IYA83_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ8585667.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            20624..21256
                     /locus_tag="IYA83_000199"
     CDS             20624..21256
                     /locus_tag="IYA83_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ8585668.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(21258..22163)
                     /locus_tag="IYA83_000200"
     CDS             complement(21258..22163)
                     /locus_tag="IYA83_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ8585669.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(22248..22901)
                     /locus_tag="IYA83_000201"
     CDS             complement(22248..22901)
                     /locus_tag="IYA83_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ8585670.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            23223..24167
                     /gene="mvk"
                     /locus_tag="IYA83_000202"
     CDS             23223..24167
                     /gene="mvk"
                     /locus_tag="IYA83_000202"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ8585671.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24160..25137
                     /gene="mvaD"
                     /locus_tag="IYA83_000203"
     CDS             24160..25137
                     /gene="mvaD"
                     /locus_tag="IYA83_000203"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ8585672.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            25150..26235
                     /locus_tag="IYA83_000204"
     CDS             25150..26235
                     /locus_tag="IYA83_000204"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ8585673.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            26232..27296
                     /locus_tag="IYA83_000205"
     CDS             26232..27296
                     /locus_tag="IYA83_000205"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ8585674.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27545..28348)
                     /locus_tag="IYA83_000206"
     CDS             complement(27545..28348)
                     /locus_tag="IYA83_000206"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ8585675.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="IYA83_000207"
     CDS             complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="IYA83_000207"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ8585676.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(29226..30233)
                     /locus_tag="IYA83_000208"
     CDS             complement(29226..30233)
                     /locus_tag="IYA83_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ8585677.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(30240..30884)
                     /locus_tag="IYA83_000209"
     CDS             complement(30240..30884)
                     /locus_tag="IYA83_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ8585678.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30984..31742)
                     /locus_tag="IYA83_000210"
     CDS             complement(30984..31742)
                     /locus_tag="IYA83_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ8585679.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="IYA83_000211"
     CDS             complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="IYA83_000211"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ8585680.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="IYA83_000212"
     CDS             complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="IYA83_000212"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ8585681.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="IYA83_000213"
     CDS             complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="IYA83_000213"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ8585682.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="IYA83_000214"
     CDS             complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="IYA83_000214"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ8585683.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="IYA83_000215"
     CDS             complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="IYA83_000215"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ8585684.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            37140..38009
                     /locus_tag="IYA83_000216"
     CDS             37140..38009
                     /locus_tag="IYA83_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ8585685.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(38135..39034)
                     /locus_tag="IYA83_000217"
     CDS             complement(38135..39034)
                     /locus_tag="IYA83_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ8585686.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(39199..40023)
                     /locus_tag="IYA83_000218"
     CDS             complement(39199..40023)
                     /locus_tag="IYA83_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ8585687.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(40034..41329)
                     /locus_tag="IYA83_000219"
     CDS             complement(40034..41329)
                     /locus_tag="IYA83_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ8585688.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(41345..41824)
                     /locus_tag="IYA83_000220"
     CDS             complement(41345..41824)
                     /locus_tag="IYA83_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ8585689.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41830..42858)
                     /locus_tag="IYA83_000221"
     CDS             complement(41830..42858)
                     /locus_tag="IYA83_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAQ8585690.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(42875..43981)
                     /locus_tag="IYA83_000222"
     CDS             complement(42875..43981)
                     /locus_tag="IYA83_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ8585691.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="IYA83_000223"
                     /gene_synonym="adhC"
     CDS             complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="IYA83_000223"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ8585692.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="IYA83_000224"
     CDS             complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="IYA83_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ8585693.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="IYA83_000225"
     CDS             complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="IYA83_000225"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ8585694.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            48952..49650
                     /locus_tag="IYA83_000226"
     CDS             48952..49650
                     /locus_tag="IYA83_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ8585695.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(49818..52457)
                     /locus_tag="IYA83_000227"
     CDS             complement(49818..52457)
                     /locus_tag="IYA83_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ8585696.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(53076..54107)
                     /gene="queA"
                     /locus_tag="IYA83_000228"
     CDS             complement(53076..54107)
                     /gene="queA"
                     /locus_tag="IYA83_000228"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ8585697.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            54300..55475
                     /locus_tag="IYA83_000229"
     CDS             54300..55475
                     /locus_tag="IYA83_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ8585698.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            55479..56120
                     /locus_tag="IYA83_000230"
     CDS             55479..56120
                     /locus_tag="IYA83_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585699.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            56117..57034
                     /locus_tag="IYA83_000231"
     CDS             56117..57034
                     /locus_tag="IYA83_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8585700.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            57031..57702
                     /locus_tag="IYA83_000232"
     CDS             57031..57702
                     /locus_tag="IYA83_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585701.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(58243..58731)
                     /locus_tag="IYA83_000233"
     CDS             complement(58243..58731)
                     /locus_tag="IYA83_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ8585702.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(58932..59198)
                     /locus_tag="IYA83_000234"
     CDS             complement(58932..59198)
                     /locus_tag="IYA83_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ8585703.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(59198..59461)
                     /locus_tag="IYA83_000235"
     CDS             complement(59198..59461)
                     /locus_tag="IYA83_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ8585704.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(59454..59726)
                     /locus_tag="IYA83_000236"
     CDS             complement(59454..59726)
                     /locus_tag="IYA83_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585705.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            59937..60122
                     /locus_tag="IYA83_000237"
     CDS             59937..60122
                     /locus_tag="IYA83_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8585706.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(60264..62087)
                     /locus_tag="IYA83_000238"
     CDS             complement(60264..62087)
                     /locus_tag="IYA83_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ8585707.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(62685..65390)
                     /locus_tag="IYA83_000239"
     CDS             complement(62685..65390)
                     /locus_tag="IYA83_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ8585708.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(65696..66643)
                     /locus_tag="IYA83_000240"
     CDS             complement(65696..66643)
                     /locus_tag="IYA83_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ8585709.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            66832..67101
                     /gene="rpsN"
                     /locus_tag="IYA83_000241"
     CDS             66832..67101
                     /gene="rpsN"
                     /locus_tag="IYA83_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ8585710.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(67277..67639)
                     /locus_tag="IYA83_000242"
     CDS             complement(67277..67639)
                     /locus_tag="IYA83_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ8585711.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(67650..68771)
                     /locus_tag="IYA83_000243"
     CDS             complement(67650..68771)
                     /locus_tag="IYA83_000243"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ8585712.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(68787..69137)
                     /locus_tag="IYA83_000244"
     CDS             complement(68787..69137)
                     /locus_tag="IYA83_000244"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ8585713.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(69267..70778)
                     /locus_tag="IYA83_000245"
     CDS             complement(69267..70778)
                     /locus_tag="IYA83_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ8585714.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(71100..72452)
                     /locus_tag="IYA83_000246"
     CDS             complement(71100..72452)
                     /locus_tag="IYA83_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ8585715.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(72605..73681)
                     /locus_tag="IYA83_000247"
     CDS             complement(72605..73681)
                     /locus_tag="IYA83_000247"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ8585716.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            73877..75622
                     /locus_tag="IYA83_000248"
     CDS             73877..75622
                     /locus_tag="IYA83_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ8585717.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(75902..76339)
                     /locus_tag="IYA83_000249"
     CDS             complement(75902..76339)
                     /locus_tag="IYA83_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ8585718.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(76323..77696)
                     /locus_tag="IYA83_000250"
     CDS             complement(76323..77696)
                     /locus_tag="IYA83_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585719.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(78018..78506)
                     /locus_tag="IYA83_000251"
     CDS             complement(78018..78506)
                     /locus_tag="IYA83_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8585720.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(78528..79130)
                     /locus_tag="IYA83_000252"
     CDS             complement(78528..79130)
                     /locus_tag="IYA83_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ8585721.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22691 a        16795 c        14460 g        25227 t
ORIGIN      
        1 atattcttta ggatattaaa attaactagc accacgggta gctattaagt aaagcatcaa
       61 aataattgat agcaactttg gtatcatcat tagaatctat ataccttttc aaaaacattt
      121 taaaaaactc atcagataaa ttgtgggttt catttggagc aaacaaaaaa gctaaaaaat
      181 tactatgtct tatttcattt ttagatagct taagtatctg aaatatattt gttttcctag
      241 tccatttaga tagtttttct tcaatatcat ccatatcctc caatagattt tttaaacact
      301 ttttttcatc tatcatgttt tttctccttg ccattattct aactaaaatt ttacgtattt
      361 gtttctatgt ctacattatc tttttaacag tatctatttt tcttttgcaa cttacttggc
      421 taccaaagca aataaaaaag ttatttataa atttctccct accactacct attgctatta
      481 tttaaaatag ggataatttt ttctcttaaa aaatatcatt atttgtggta tggagccccc
      541 gcgttgatcc ggaaggcacg gtagatttgc tcgacgagga tcaggcgcat caattgatgt
      601 ggatacgtca ttttgccaaa agagatctga gcattgcttc ttttcatgac ggcttcgctg
      661 agtcctaaag aaccgccgat aacaaaaaca aggtggcttt ttccttggat acctaattgg
      721 tcgatttgtt tagcaaaaga ttcgcttgtg ggatttttcc cttcaattgc taagccatag
      781 acgtattctt gttctttgat tttcgctaag atccgttctc cttctttttc ttttacttgt
      841 ctcatttgcg cttcgcttaa attctccggg gctttttcgt caggaacttc gatcaattcg
      901 attttcgcat aggcattcag ccgtttgaca tactcattga taccttggat caagtatttt
      961 tcttttaatt ttcctactga aataattttg atattcatgt tttctctcct ttgatcttca
     1021 cgctcacttt gtttattata atcaattttt tcgtctatat gtttattttg actatttttg
     1081 ttaaaaagat ttccccaaca gttatgcaca acatccacag gtttatccac agaaaacagc
     1141 gttgttttac cttatttcat cacattcatt aggcaaacat acgttttata agaatttatc
     1201 cactttatac acaggctgtg gatatgtttt atcccttatt tttccaatcc tatccgcgta
     1261 gttttacaca atatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt
     1321 ttcctaaagt ttttcataaa aagttcaaag tctcaaagta gactaaagtc aacaaataag
     1381 actatactaa cagtagataa atgaatgtaa ttggaggagg aacacatgga tagaaaaaat
     1441 gtgacaccaa agatgaaaaa aaataaaaat aatagcctct ggcgtaagtt gggtctgggg
     1501 cttgttggag ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc
     1561 actggcaaca atagttctac tgctacaagc ggcaatcaaa attctgccgg tgaaacagtt
     1621 gtggaaaatg tcaaagtcaa tgtagattct gatatcacca aagcggtaga taaagttcaa
     1681 gacgctgtcg tttctgttat caacttacaa agtcaaaacc aatcaagcgg ttttggcgga
     1741 ttattcgggc agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt
     1801 gaaggaagcg gcgtaatcta caaaaaagac gggaatactg cttatgtagt cacaaacaat
     1861 cacgtagtag atggacagca aggtttggaa gtcatgatga aagacggtac gaaggtaaaa
     1921 gctgaattag ttggaacaga tgcctattca gatttagcgg tgttgaagat caattcagac
     1981 aaagtcgaaa cggttgcctc ttttggggac tcaagtgctt tgaaggttgg tgaacctgcg
     2041 attgcgattg gttccccatt aggatctgaa tatgcgaatt ctgtcacttc aggaatcatt
     2101 tcttccttga accgacaagt aacaagtacg aatgaatcga acgaaacagt caatatcaat
     2161 gcgatccaaa cagatgcagc gatcaacccg ggaaattctg gtggtccttt agtgaatatc
     2221 gaaggacaag ttatcggtat caactcaagt aagatcgcaa gtacttctga atcgacttct
     2281 aatgtaagcg tagaaggaat gggatttgcc atcccaagta acgatgtagt gaatatcatc
     2341 aaccaactgg aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac
     2401 ttatctgccg tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact
     2461 aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg cagaaaaagc tgggttgaaa
     2521 caatacgacg tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa
     2581 tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg
     2641 aaagaaaaaa caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa
     2701 aatagtgaaa attaataaaa aagaggttgt gacaaagact tgtcacaacc tctttatttt
     2761 ggaaatactt ctccgactac ggcatcctca ggcaatacta agattccttt tttttctgga
     2821 gcatttggca aatgcaattc acgagcagaa caaatcattc catagctggc tacccctcgc
     2881 aaagttcctg gccagatcat cattccatct ggcatcatgg ctcctggttt tgcaacgaca
     2941 actttttgtc cagcttcgat atttggtgca ccgcaaacaa tttgcaatac tgtcccattg
     3001 tccacttctg tctcagtaat cgataaatgg tcagagtcag gatgcgggac gcatgttttt
     3061 acatacccga caacgatctt cggttcatga tcagcttcaa gctgttcttc gaatcctgct
     3121 tctttaatca atcgattcaa tttcgctaat tcttcatctg ttaagcttac ttggccgttc
     3181 ccttcaattg ttagatgatc ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt
     3241 tcatctttga tccgagcaac attccattta cgttcgacag tgttttctgt tcctttatcg
     3301 tcggctatga taaccatcaa agtgtctccg acgtgtgctt tgttataagc aaaaatcatg
     3361 ttctttcctc cattatcctt tgctgagatt tatcttaaca aaaaattgca ttcactgcat
     3421 gagatttttt tcatgcttta tttccgaaga agcttcttct ggaacaccat ttattcgtaa
     3481 caagggactg tgacaagact tttgtcacag tccctttact gtttgattaa aaaggttata
     3541 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
     3601 caaggtgaat gacgtatgac gtagctggct acacttcctg tcataaagcg ttcgactgca
     3661 tttagtccag actgcccaac catgattaga tcgattgcat atttttttgg caatttgact
     3721 gccatggatt cacgaacaga gccgtatgca ataattcctt ctatatttgt gtaatcaaca
     3781 gattttccat attctttaca ttcttcgatc atttgcttgg ctgtttcagt ttgctgatcg
     3841 actagatttt gatcaatagg agcatagccc ataaaattat aaaattgctg atcgattacg
     3901 tttgctacat atactttccc attattacgg cgagccactt ctaacgcttt tttgaaagca
     3961 gcaagtgctt gttcactacc gtcaatacct actaaaatgt tcttatattc ttgattcatc
     4021 ttgacccctc ctaacagatg tcagcctttt attacacttt ctaggaacga ttcgatttcc
     4081 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
     4141 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
     4201 tctacttcaa taaattgcca atcagaaaag tcattttcga tttcaggcat ttgcggtttg
     4261 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactttt tccagtttct
     4321 acgtagcctg ctaattcttc taaactattt gggataatca ttctttttcc tctactttct
     4381 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
     4441 ctttttataa aaaaaatctg acaaaaggtt gcttttgcca gattttatag tttcactatc
     4501 gcgttatttc ccgtatacga tcgtgttcac tgttgattta tctaaacctt tcaatgtttg
     4561 aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg
     4621 gatataacgt cccactacgc caatcactgt acttgggatt ccttcatttt gtgtatgagc
     4681 agcacctgca tctgtaccgc cttttgaaac aaagtattga taagggatgt tgtttgtttc
     4741 agcgatatct agcaagtact cacgcaaacg aggaagcatg atcaaacctg gatcttggat
     4801 acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca tttttcgtca ctgtgtcatc
     4861 tgctgcagag cagtctactg caaagaacag atctggcttg aatttattga cagaagcttt
     4921 agaaccacgc aagcctactt cttcttggac attcgcgcct gcaatcaatg tatgacctaa
     4981 tgtttcattt tgtaacgctt ctaaagcttc taataccatt gtacagccat agcggttgtc
     5041 ccacgatttg ctgatgatat tcttcccgtt ggctgttttg atcgtttcag cgtaaggaac
     5101 gattgtgtct cctggaagaa caccgtaact cattgcctct tctcttgatt caaacccagc
     5161 atcgaacaaa atatctgtta cttctactga tttttgtccg cttgttcctc gtaataaatg
     5221 tgggggaacg gaagaagaaa tacatggata attcttacca gtagaggttt taagtgtaaa
     5281 acgttgagca gaaacaacgt aagggttcca tccgcctaga ggtacgactt taaaaagccc
     5341 gttttcttgg atttgtgtca gcatgaatcc gacttcatcc atatgtgctg cgaccattac
     5401 acgaggtgca tccgcttctt catggtggcg cagcccaaaa atgccgccca aaccatctaa
     5461 ctgtaactca tctaccagcg gttccatatg actttccata taggcgcgaa cgtcttgttc
     5521 aaaaccgctt gttccttgta attctgttaa ctctttgatt cgttgaaatg ttttttcttc
     5581 catatgctat tcgtctaaat ttcagactaa actcctctca gacttatatt gatctgcgga
     5641 taaaatatga ttttttctgc agaccgatcg tatttattat agcttatttt ttctggtagc
     5701 ggttatttat ttcaaagaaa atatgagagt gtggtaaaat aaacacaaag agtagcaagt
     5761 gagaagaagg aggtatatcc acatgtatga agaaagcgaa ttaagctatt ttaaaggcgg
     5821 gttagccgtt gggatcggtc taggattatt gagtggggta gcttcaacga tttggtatca
     5881 tcgcaaaaaa gtgttatcag cagaccaagt gcttgaaaat atcaaaacat cttttttaaa
     5941 agaaggcgca atcgaaggtg cttggatctc ttttgaaaaa gaacctattc ggaaattcgc
     6001 cgttcggtcg agtgcttatc ggggtgggct ttcccggata gaagatggcg aaactgttta
     6061 ttatgaattt ttagcagatg ctcatacagg aaccgtatta gacatttcaa gaaaaaaggg
     6121 cgcaatagat taatacgtca aaaaaatgaa ataacgaata cgcacccact atctaaaaga
     6181 gaggtatcct atccttcttt tatagtgggt gatttttata aaaatagggc tatttcctct
     6241 tggaatagct agtatttgta gttattatac ggtattattg aaataagatt agttttatta
     6301 tataaggaga gtgacaaatg gaatcacgtg aaaaaattgc cgtgctcatt ccttgttata
     6361 acgaagaggc gacaatttca actgttattg aagattttaa aagggaatta ccagaagcag
     6421 atatttatgt ctatgataat aattcgaccg atcgaacata cgaattggcc gttcaaggcg
     6481 gagcaatcgt aaagaaagaa ccacgtcaag gaaaagggaa tgtaatccgt caaatgtttt
     6541 ttgatattga tgccgattat tatttgatgg tagatggtga tgatacttat cctgcagaag
     6601 ctgcacatgg tttgcttgag aaattacgtt ctggcgaagc agatatggtc attggcgatc
     6661 gtctatcaaa tggaacctat tttgatgaaa ataagcgtcc tttccatgat tttgggaata
     6721 atctggttaa aaatacaatt acttacttat ataaaacaaa aattcgcgat gtcatgaccg
     6781 gctatcgagg attcaatcga atttttgtca aaagcttccc tattatgagt tctggttttc
     6841 aaatcgaaac agaattgacg attcatgctt tagataaaaa attcaagttc gttgagattc
     6901 caatcgatta tcgcgatcgt cctgaaggta gcgaatcaaa attgaataca ttctctgacg
     6961 gattcaaagt gatcatgatg atcatcaaaa tgtgcaaaga ttataaacct ttgcttttct
     7021 ttgggatctg gaccatcttc ttctttttat ttggattggt catggggatt cctgtgattc
     7081 gtgaatatat gctgacaagc tttattacac gcatcccttc tgcgatttta agtactggat
     7141 tgatgacttt agcccttctt tcattagtaa cagggttgat tttggatacg gtcgtcacaa
     7201 atgccaagaa agaatatgaa ttgaatttat accatgtcta ccacgaatat caaaataaag
     7261 aaatgaagta ggaggccttt tttatgcata agaaaaagga gaatctattt cctacttttt
     7321 ttgcaacttt atttgcgtca ggttttgctt tttttacttc ttatggtcta ggaactttga
     7381 atccaacagt aatcattggt ttagccatgg ttttgctact tgcttttaag caatatggaa
     7441 tagagttgtt ttcaataaaa aacaaagttg cagctattgc ccgattgttt ttttctgcag
     7501 cttttgctct tacactttct attggcagac agatacaatt tcagtatgtc tcagcaaact
     7561 atacaaagac aacgattgct gatggaagta ttttttactt tcttgtttat ttctccattt
     7621 ttgtgtattc ttttttattt ttacaatcga tttatttatc aatgccaaaa ctggaaacat
     7681 tttttactgc tcaaaacaca caggctttct cttcaagaaa atatttttta ttctttactg
     7741 tctttttagt tctgtgttgg cttccttatc tgttggcatt gtatcctgga gtggttttgc
     7801 ctgattcgtt aagttcggtc gcccaatcta tgggtgatgt tcctataagc aatcatcatc
     7861 cagtcttatt caccttactg gtcaagctat ttgttcatga tttaccaact gatacgatca
     7921 atcacggtgt atttttattt tcgatttttc aaacattgat aacagcttca gcgataagtt
     7981 attctctttg ctggctgtta aaaagaaaca tccatttttt tccggtactc attagtttat
     8041 gctattttgc atttgcaccg gtttttccaa tctacgcctt gaacatgcaa aaagatactt
     8101 tgttctctat cgcttgcttt ttattttcct tagcggtttt tgactttatt tttcaacgag
     8161 aaaagccaac gattctcaat cagatttacg taatcatcct tgctttactg accacctatc
     8221 taagaaataa tgggatttat gtagtaaccg gcacaggttt gatttgcggc ttgattattt
     8281 tgaaacaaaa aaggtatcaa gcattctttc aagtggtgct gggttataca gtgattgctg
     8341 gactactcat ccacccgttg atggctcgtt atgctattcc tacagaaact gctgaacaat
     8401 taggtgttcc tttgcagcaa atttctagaa ctattgttat ggatggtgac ttgtctaaga
     8461 aagataaagc attcatgaat cagctactcc cgataaaaga ttatgaagcg tattctccta
     8521 gtcttgcaga ctcaatcaag tggcataaaa attttaacaa tgagtttttg aatacacata
     8581 aaaaagaatt tctatcacta tgggcaagaa atttaccaag taatataaaa atctatacag
     8641 atgcttatat tttagaaacg ttcgggttct gggtacctgg tacaaaaaat agttacggct
     8701 ttttagatac tcgtgtaaac acgaataatt atggtatgaa gcaagtagat ttgattgaac
     8761 atcttactgg attgtcagga atgaaaaaaa tcatcgatgc tcgtgatttc tttggttccg
     8821 gcacattact gtggttagct atcggcagtg cacttttagc aatctttaag aaaaaatatc
     8881 ggtatttact cctttttctt cccggtatgc ttgtttttgc tactattatg attgctactc
     8941 ctgtggcttt tagtttacgg tatgtgtttg ttttagcatt aggtctacct gtttatatcc
     9001 tctctccttt tttactagat aaaagagaaa aaatgaaaac agatatagga gatactcatg
     9061 aaactattta aaaagatatt ttttgctttt gttctcttga tcgctagcta ttgcgtacta
     9121 ctgacaatta cttttttcat tccgcagcac tctattgaat cgaatgcaga aaaatctcta
     9181 cagatggttg aaaaagaagg aatgtatcct tctatcaatc aaggaaatat gctaggcact
     9241 cggttagata attttactga tcatttgatg atacgaaaaa caaaagcaga tcctgaattg
     9301 aactccttgg agaacgcgat gtctatggcg gattatcccc gttattggca tggttatcag
     9361 ctttttttac gacctttact gctagggatg agtttgggga gtattcgaat gatttacgca
     9421 gccattcttt ttctgctgat tggattgacg agctactttt taaccaagtg tagcgacatc
     9481 tatttaacga cagccttgtt gatttcgctt gctataggaa atgctgcaac ctttttcttc
     9541 agtatgcaat tttcaaatat ttggattttg actttattgt ctgtattact atttttaaca
     9601 aaaccacaac tctccaacac caaccaacaa ttattcttgt atttctttgt cattggttct
     9661 ttagctaact tttttgactt gttgacaaca ccggtcatct cttgggggat acctgtgatc
     9721 attatttatt atgtaacaaa taagtattta atggataaac gcagttcatt gagcaaacaa
     9781 gtaggcagtt ttgtttttac cggtatcttt tggggattag gctatggttt gacatgggtg
     9841 gctaaatgga tcctatcatc gatcatattg aacaaaaaca ttgtaaaaga tgcaatcaac
     9901 caaatcctct ttcgaacgga aggaaacgac aaatacccgt tgcatcgatt agagatgctg
     9961 aaaagcaata ttagattgat gtatccaaaa gtagcaatat tattgctatt gattacttgt
    10021 cttgtttttc tctattttgc ttatcgtaaa cgaaatagct ggcgtccgtt ccaacttcta
    10081 gttttgtttg ttttagctgc tgtaacgccg tatatttggt acaatatttt agctaatcac
    10141 tcacagattc attattggtt tacgtatcgt acgcaaatca tcacaagttt tgctatactt
    10201 agtgctttcg cctttttgat ccctcctgag aaatcaaaag acgaactaaa ttacttttaa
    10261 gaataccgat gtactaattc atttttaaac ataaaaactc cagctgtcag agatagccgg
    10321 agttttttag tatttttatt tcgccaaacg atagatggca tctgcataga ttgctgttgc
    10381 tctgaacaag tcatcaagac tcataaattc gtttgcttga tgcattgtat cagtatatcc
    10441 tgggaacatc gctccataag caacgccacg tttcaacaaa cgtccgtaag ttccgccacc
    10501 gatgatttgt tcgtagcctt tttctccagt gtggtcttca tagacttgca ataatgtagc
    10561 caccaatgga tcatccattg gtacgtaatg tggttccatt acgcgagcac ctcgagtaac
    10621 tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt tgcaatccgt ctactgttac
    10681 accttttgga aaacggaaat tcatattgat gaagttcgct tcctctgtac cattttcttc
    10741 aaaagcaaat agtccagcat tcattgtcaa tttgcccatt ttttcatctt cataagcgac
    10801 tcctagtttt tctccataaa aatcttcatg gacataagca gctgctacat ggataaattg
    10861 tttagcagat cctaggaatt catagttatc taagaacaat gccaagaaag aacctgcatt
    10921 gataccagat tgtgggcttg caccatgtgc gcctttacca actaattcga tttttacata
    10981 cgtattgtcc gcttcaatcg ttccgcttac tggatttgct tcgatgaatt ggtaaaatgc
    11041 ttcttccatc gcgatagctg catctgcact tggtacttga agtgctgcag ttgctgttcc
    11101 tggcaccata ttttcgcgaa gtccggaaac aaaagatttc aagacataat ctcctgcatt
    11161 gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt tcaccattga tgattgggaa
    11221 ttcagcatct ggtgagaaac caaaatctgg ttcttcttca tgttcgaaat aataagccat
    11281 atcgccccag ccgctttctt catcgctgcc gactacgaag cgaacttttt ttgatagttt
    11341 caagtcaagt tctttaatga tcttcatcgc atagtaagct gccatacttg gacctttgtc
    11401 atcgcttgat ccgcgtgcaa agatctttcc atccttgatt actggttcat aaggatctgt
    11461 gtcccagcca tctcctgcag gaacgacatc catgtgtcca aatatcccta gcgtttcgtc
    11521 tccttcgcca agatcaatat gccctgcata gttatcgaca ttttttactt taaatccatc
    11581 acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga ggtccaggtc caaatggtgc
    11641 atctggtgtt acttttgaat catcgcgctc gctgttgaca cgtaaaagat ttttcaagtc
    11701 ttcaagcaag tcttctttac gtgcttctac ttctttttgc caatcaattg tcataaagtt
    11761 aatcctccac ttatttaagt cttcgtgttc atgataccac aaattacaga aaaaaatagt
    11821 tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc taaaaaaaat atacgctata
    11881 atgagtaaaa ggaggttttt cgatgctgat aaacgaacaa ctagtgaaaa gagtcatcac
    11941 acctcgtcca aagatttcac ataaaggaac atttggacgt atcgttttga ttggcggcaa
    12001 tgaacaatat ggcggagcaa tcattatgag tgctttagct gccgtacaca gtggagcggg
    12061 tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc atctgccaga
    12121 agcaatgatt gtagattgga cggatactga ccagtttctt tccgtaataa agcaagcaga
    12181 tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac gtttcaaaca
    12241 agtactagaa aaacaatcaa aagaacaatg gctggtgatt gatggctctg ctttaagttt
    12301 acttgttgct aatcatcttt tgctacccta tcccgaacaa actgtactta cacctcatca
    12361 aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata aagaaaacaa
    12421 aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga caaaaattta
    12481 tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa caggcggcat
    12541 gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc aaaatgacca
    12601 aacgattgca gcagctgttt atcttcatag tttcattgga gaccagctgg caaaagaaca
    12661 atatattgtt ttgccgacac aaatcagcga agcacttccg aagtggatga attatttttg
    12721 taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt atgtgcgaga
    12781 acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt atatccgctg
    12841 gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata aaaaaatcga
    12901 agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag aaatgatcca
    12961 ttacggagaa aaagtgagca tccacccaac tatccaaaag tatacgggaa cacggttgga
    13021 ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg gcagtagtaa
    13081 acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag tcaatccgga
    13141 gttagaacag ttattccaag cgtacgcttc tttggatgat gtaaattctt aataaatacc
    13201 gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga aaaaagcagg
    13261 tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact ggctgtcgca
    13321 ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa tccgaagctt
    13381 ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca gattgattca
    13441 atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt gcagactgtg
    13501 tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat ctgcatagaa
    13561 aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct atacgtaaac
    13621 aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg ttagaagaac
    13681 aactagctat cgggcgtaca ccgattcgag aagcactgat ccaactgcgc aatcaagagc
    13741 ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc cgatctgctt
    13801 cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa tgcagtgcga
    13861 aaatgaccaa gcaagatcaa gctaaactag aaaaaatact agaacaaacc gatcaggcag
    13921 tgatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac cgaacttgtt
    13981 atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt acgcacttga
    14041 accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga gtattagatg
    14101 aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc ggatttcttt
    14161 gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat aattaccccg
    14221 attattttga agatattcag gacagaccca tcaaataaga ggttttggaa tcagcgtttt
    14281 gacctgagta ttagagaaat aaacggcaaa atagttcctc atattttgcc gttttttttg
    14341 agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg aaaaagctgc
    14401 cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt taacttggct
    14461 gttcttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc gtgagcctgc
    14521 cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    14581 ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa aaaaggactg
    14641 tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc gtacatcttt
    14701 tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta tatggccaaa
    14761 gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct tttgaaaaac
    14821 tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa aaaggcaacc
    14881 gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct tcgatgtggt
    14941 gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc gaagctagtc
    15001 ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca tcttctgcat
    15061 aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg attggaacag
    15121 ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg atgctacatg
    15181 ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg gacaaaggaa
    15241 tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct tcggggatct
    15301 gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc ggcaacgacg
    15361 atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag ctgtagttgt
    15421 tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata aaattctggt
    15481 cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc gacagctgtc
    15541 atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac cttgataaat
    15601 cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg ttcgccgcat
    15661 acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa tcgcatatcc
    15721 tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc ggttgctttc
    15781 acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc gatttctacg
    15841 tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat cgcttcattt
    15901 ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac aacatctcgg
    15961 accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg aatcgaatat
    16021 ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc ccctacacct
    16081 acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa aatcaccggt
    16141 tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac cgtcgtatga
    16201 caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc ataagaaaaa
    16261 tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat agtcacttct
    16321 tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt aggagagaaa
    16381 aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata atctagaccg
    16441 gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc ccgtaccatc
    16501 gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt tgggtggcca
    16561 cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat attggttgtt
    16621 gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa agagcccatg
    16681 actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat ttcattgatt
    16741 tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc tgcttctcgt
    16801 acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga tgcattgatt
    16861 gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga agccatcgtt
    16921 gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc cccattatct
    16981 actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa agaagaaatc
    17041 gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt gacaatctct
    17101 atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt tgctaaaaaa
    17161 ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc tccttcaatc
    17221 agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc tggactgata
    17281 ttattttccc gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac tccacggaca
    17341 tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc attatcgatt
    17401 gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa acgtttcatt
    17461 ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag gcgttcccgt
    17521 acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt gggatggatt
    17581 tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca acccaagatt
    17641 gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg aacaaaaagg
    17701 ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag cctgaaccga
    17761 ctagattcat tggaccgcag aaaaatccta gtggtgtcaa tatagcaaat acgattgcca
    17821 agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa ggagaagcat
    17881 acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt ggtgcaacat
    17941 ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta taacctccgc
    18001 gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct aagatgattg
    18061 gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat gagatcattg
    18121 gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc tttttgttca
    18181 ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca atcataccaa
    18241 tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca gcataaatcg
    18301 tttgatattg tttgaaattg acgatattag caaagattcc agccataatc gagcccataa
    18361 atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc aacacaataa
    18421 ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca gcggttacaa
    18481 tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact acctttcgaa
    18541 tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca ccaaagaaga
    18601 catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata tgcgtcaaca
    18661 catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt ccgactagcg
    18721 ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg tagcctttta
    18781 cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata ataatatcac
    18841 tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata tcagtcaaac
    18901 tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg ttcagttttc
    18961 aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg tttcatttga
    19021 tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct tctttcctct
    19081 ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca atgcagtaaa
    19141 tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt cgatttcagc
    19201 aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag ctgctgcgtt
    19261 gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat cgcgatatgc
    19321 gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt gttttgaacc
    19381 gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta ctgttccacc
    19441 tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat tctgttattt
    19501 gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg ccactagaaa
    19561 ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg aaacggtgac
    19621 ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa cgttttgctg
    19681 atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat ctttcgttat
    19741 ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt cgtttttcgg
    19801 tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca ctgttgcaat
    19861 atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt tatgggtaat
    19921 ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca agaacttacg
    19981 tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta agcgaacttc
    20041 ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt gttgttcgaa
    20101 acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag gggcaactaa
    20161 cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg tcttaactcc
    20221 taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc cgtcgttaac
    20281 catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag aaaagcttac
    20341 cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac ttcacctaat
    20401 agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga agcgggtgct
    20461 tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg tcaatgtttt
    20521 tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc tagcagtgat
    20581 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
    20641 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
    20701 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
    20761 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
    20821 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
    20881 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
    20941 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
    21001 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
    21061 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
    21121 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
    21181 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
    21241 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
    21301 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
    21361 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
    21421 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
    21481 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
    21541 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
    21601 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
    21661 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
    21721 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
    21781 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
    21841 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
    21901 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
    21961 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
    22021 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
    22081 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
    22141 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
    22201 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
    22261 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
    22321 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
    22381 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
    22441 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
    22501 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
    22561 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
    22621 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
    22681 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
    22741 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
    22801 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
    22861 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
    22921 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
    22981 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
    23041 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
    23101 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
    23161 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
    23221 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
    23281 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
    23341 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
    23401 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
    23461 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
    23521 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
    23581 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
    23641 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
    23701 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
    23761 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
    23821 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
    23881 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
    23941 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
    24001 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
    24061 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
    24121 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
    24181 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
    24241 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
    24301 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
    24361 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
    24421 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
    24481 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
    24541 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
    24601 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
    24661 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
    24721 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
    24781 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
    24841 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
    24901 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
    24961 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
    25021 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
    25081 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
    25141 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
    25201 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
    25261 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
    25321 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
    25381 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
    25441 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
    25501 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
    25561 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
    25621 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
    25681 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
    25741 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
    25801 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
    25861 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
    25921 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
    25981 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
    26041 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
    26101 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
    26161 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
    26221 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
    26281 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
    26341 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
    26401 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
    26461 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
    26521 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
    26581 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
    26641 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
    26701 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
    26761 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
    26821 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
    26881 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
    26941 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
    27001 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
    27061 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
    27121 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
    27181 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
    27241 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
    27301 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
    27361 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
    27421 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
    27481 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
    27541 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
    27601 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
    27661 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
    27721 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
    27781 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
    27841 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
    27901 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
    27961 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
    28021 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
    28081 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
    28141 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
    28201 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
    28261 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
    28321 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
    28381 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
    28441 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
    28501 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
    28561 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
    28621 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
    28681 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
    28741 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
    28801 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
    28861 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
    28921 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
    28981 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
    29041 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
    29101 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
    29161 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
    29221 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
    29281 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
    29341 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
    29401 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
    29461 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
    29521 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
    29581 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
    29641 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
    29701 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
    29761 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
    29821 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
    29881 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
    29941 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
    30001 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
    30061 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
    30121 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
    30181 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
    30241 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
    30301 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
    30361 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
    30421 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
    30481 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
    30541 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    30601 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    30661 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    30721 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    30781 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    30841 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    30901 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    30961 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    31021 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    31081 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    31141 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    31201 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    31261 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    31321 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    31381 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    31441 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    31501 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    31561 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    31621 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    31681 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    31741 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    31801 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    31861 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    31921 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    31981 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    32041 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    32101 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    32161 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    32221 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    32281 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga aacttccaaa
    32341 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    32401 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    32461 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    32521 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    32581 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    32641 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    32701 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    32761 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    32821 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    32881 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    32941 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    33001 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    33061 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    33121 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    33181 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    33241 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    33301 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    33361 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    33421 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    33481 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    33541 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    33601 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    33661 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    33721 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    33781 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    33841 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    33901 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    33961 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    34021 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    34081 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    34141 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    34201 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    34261 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    34321 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    34381 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    34441 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    34501 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    34561 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    34621 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    34681 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    34741 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    34801 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    34861 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    34921 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    34981 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    35041 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    35101 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    35161 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    35221 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    35281 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    35341 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    35401 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    35461 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    35521 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    35581 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    35641 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    35701 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    35761 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    35821 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    35881 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    35941 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    36001 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    36061 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    36121 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    36181 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    36241 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    36301 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    36361 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    36421 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    36481 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    36541 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    36601 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    36661 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    36721 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    36781 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    36841 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaacggtgta cttcttccaa
    36901 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    36961 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    37021 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    37081 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    37141 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    37201 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    37261 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    37321 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    37381 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    37441 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    37501 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    37561 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    37621 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    37681 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    37741 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    37801 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    37861 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    37921 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    37981 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    38041 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    38101 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    38161 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    38221 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    38281 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    38341 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    38401 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    38461 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    38521 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    38581 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    38641 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    38701 aacaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    38761 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    38821 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    38881 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    38941 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    39001 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    39061 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    39121 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    39181 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    39241 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    39301 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    39361 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    39421 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    39481 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    39541 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    39601 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    39661 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    39721 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    39781 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    39841 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtgtttc
    39901 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    39961 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    40021 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    40081 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    40141 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    40201 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    40261 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    40321 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    40381 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    40441 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    40501 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    40561 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    40621 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    40681 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    40741 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    40801 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    40861 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    40921 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    40981 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    41041 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    41101 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    41161 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    41221 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    41281 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    41341 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    41401 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    41461 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    41521 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    41581 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    41641 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    41701 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    41761 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    41821 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    41881 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    41941 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    42001 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    42061 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    42121 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    42181 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    42241 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    42301 catcacacgg atcttctttc cctccaaatc ttccggcgta tggatcggac cgtctaccat
    42361 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    42421 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    42481 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    42541 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    42601 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    42661 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    42721 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    42781 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    42841 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    42901 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    42961 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    43021 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    43081 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    43141 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    43201 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    43261 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    43321 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    43381 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    43441 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    43501 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    43561 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    43621 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    43681 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    43741 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    43801 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    43861 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    43921 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    43981 tctttacgcc acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    44041 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    44101 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc
    44161 aagttctttt tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt
    44221 aagcatcaat gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg
    44281 tacattggtc ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt
    44341 gtgtgactcc ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa
    44401 cagccgttgc taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt
    44461 ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat
    44521 gtttttgtgg atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc
    44581 gtccatgagg gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta
    44641 ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg
    44701 attccatatc aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt
    44761 gtaggctcaa tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat
    44821 gtgttaacac atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt
    44881 gaggatcgac gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat
    44941 gtgtttcact gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg
    45001 tcgggataca tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga
    45061 tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc
    45121 acattccctt agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct
    45181 tgaagtctgc catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa
    45241 catctgagaa gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg
    45301 tatcgcagta accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt
    45361 tttccatttt ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt
    45421 tgaaccattg catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag
    45481 cagagacgtt acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg
    45541 gaatcatttc gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa
    45601 tacgacaagc tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga
    45661 taactgctgt atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat
    45721 ggtccgtgtt gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg
    45781 gataatctgg gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac
    45841 tgattcctgc caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg
    45901 catattttcc ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga
    45961 tataaacttg gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga
    46021 tcacagcttg ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat
    46081 cgttcactgc acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac
    46141 ctacacctaa agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg
    46201 tcgctaagac gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag
    46261 gatgttcgat ccattggata caattttcag gagctccggc agcgatagca gcatcacgta
    46321 ccactcgtgc tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg
    46381 gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg
    46441 ttggagttac tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga
    46501 catcttcatt gatcacacca actgttttat catgtttgat attatgccaa atatattcgg
    46561 acgcatacat atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag
    46621 ccatcttcgc taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat
    46681 ggtctacttt ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg
    46741 ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt
    46801 tagccatcat tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc
    46861 atacaatatt tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta
    46921 aatataaaat tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta
    46981 ttatttatat gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc
    47041 tttctttttt gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata
    47101 agctttgata cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca
    47161 gtttttactt caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca
    47221 tttgttacga cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag
    47281 atcccttcgc agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca
    47341 tgtaatccgc cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga
    47401 cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg
    47461 ataaacatca caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta
    47521 caaaaaacac tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag
    47581 attttccttt agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct
    47641 tctctaaatt caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc
    47701 aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg
    47761 atcaaatggc ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca
    47821 tcaattggac ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa
    47881 gtcatacatg tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact
    47941 ccacggataa caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt
    48001 ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt
    48061 tccccgacag ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc
    48121 gcgctttgac gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga
    48181 cggtctggat tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact
    48241 gattttttca cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg
    48301 atatcagagc ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc
    48361 gaaccgttca agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt
    48421 aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat
    48481 ttatgcagtc cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat
    48541 gtattactta aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt
    48601 actgtagcta atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg
    48661 atcaattccc ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg
    48721 gcaggttgcg tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat
    48781 cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat
    48841 tctttttttt atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata
    48901 ttttttgata catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct
    48961 tctgcagaat taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt
    49021 ttactaaatc taatccctac attgattggt attgctacta ttcttcttat tatgattcct
    49081 attatcttgt ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact
    49141 tcaggaggcg gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg
    49201 ttcatgagcg gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc
    49261 gaaccattca aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc
    49321 ttagctgtgc tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga
    49381 atcattaaaa gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa
    49441 acaggacaaa cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt
    49501 tataaaggac aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta
    49561 gcaactgctg gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca
    49621 ttttatgaac aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg
    49681 gcaggagaac accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag
    49741 gaggtgtgac aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc
    49801 aacttagaaa cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc
    49861 agttcattca ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa
    49921 ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta
    49981 atagctaatg ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg
    50041 aagatcgatt ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc
    50101 aagaatgcaa cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg
    50161 acataactac ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg
    50221 gcaatttttt gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga
    50281 tccatgatgc cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca
    50341 actacattga caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc
    50401 gcaatcaaaa caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta
    50461 cgaatattct gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg
    50521 tcatccgtca aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg
    50581 gcacacccaa cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc
    50641 gctacgactg cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt
    50701 acccgagcat agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga
    50761 tgcagttctg atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct
    50821 atggcactgg cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt
    50881 ttcgctcgct tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga
    50941 ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca
    51001 taagtagcat aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg
    51061 atcgccgctt gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct
    51121 ttggtgattg agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca
    51181 gaatcaaaag gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga
    51241 taattctctt caacagccct tacgatggct acttctgttg gattaccttt gataacaagg
    51301 tcgtcatctt cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc
    51361 atcgcgctgt tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc
    51421 cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta
    51481 gcggttccta gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc
    51541 ttctgcacac cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct
    51601 gcaacagcca aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca
    51661 ccaatcacaa atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc
    51721 tgattcaatc ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg
    51781 atcttaccca tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca
    51841 gcaaccgtac atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca
    51901 gcaataaatg ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct
    51961 acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct
    52021 aaaacaagaa tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct
    52081 cgtatgacag ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg
    52141 tttccttctt ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt
    52201 ccttcaaata aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct
    52261 acaagaagga tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat
    52321 tctttcggcg cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct
    52381 tgtgaagaca acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc
    52441 tgcttgtatt cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat
    52501 tttactataa catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa
    52561 gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata
    52621 gatgtaagga aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa
    52681 gcaaacaaag ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta
    52741 taaatagtga atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg
    52801 atacgtaaca attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca
    52861 aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg
    52921 ctttgcatgt atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac
    52981 aagaaagccg agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct
    53041 tttttgttat tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca
    53101 aaactaaaga aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct
    53161 cttcctgcaa aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt
    53221 gagaaagctt ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg
    53281 ctgtctgctt tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa
    53341 gttgttccga cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc
    53401 gcttcttccg tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg
    53461 ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc
    53521 tttgcttcga tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca
    53581 gcagaaccat tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct
    53641 ttgatatatg gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct
    53701 tcatactcaa agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc
    53761 aatctgccat caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc
    53821 aatgtctccc acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca
    53881 gtatctggtt tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc
    53941 gcatcaccag gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca
    54001 gttttccgat ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg
    54061 attaattctt ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact
    54121 ttctatcatg tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt
    54181 tttcacttct attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt
    54241 gttttaggca tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca
    54301 tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg
    54361 tcaatctttc atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa
    54421 aaacgacgac gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga
    54481 ttgatggaaa atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg
    54541 ttatccaaag tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc
    54601 aaaaattgtt gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc
    54661 ttgtggaatt gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc
    54721 agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg
    54781 agccattcgg tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt
    54841 tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta
    54901 aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag
    54961 aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc
    55021 tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca
    55081 cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag
    55141 atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt
    55201 tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt
    55261 acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga
    55321 aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat
    55381 tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag
    55441 agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc
    55501 acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga
    55561 tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg
    55621 ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg
    55681 cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt
    55741 attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt
    55801 atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat
    55861 tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg
    55921 catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg
    55981 gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag
    56041 cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta
    56101 gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct
    56161 tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc
    56221 gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt
    56281 tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa
    56341 ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga
    56401 tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt
    56461 gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctatg gttttgaaaa
    56521 tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag
    56581 cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg
    56641 taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt
    56701 acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct
    56761 tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg
    56821 tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac
    56881 gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca
    56941 ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt
    57001 cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt
    57061 caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt
    57121 cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg
    57181 atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc
    57241 cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc
    57301 aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat
    57361 accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact
    57421 gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc
    57481 ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct
    57541 ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt
    57601 ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt
    57661 ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa
    57721 aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca
    57781 ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat
    57841 ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa
    57901 gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa
    57961 ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct
    58021 tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc
    58081 cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata
    58141 caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct
    58201 cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc
    58261 gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg
    58321 atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc
    58381 attgccgttg atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg
    58441 agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact
    58501 ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa
    58561 ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc
    58621 tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc
    58681 cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc
    58741 tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa
    58801 aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt
    58861 agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg
    58921 ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc
    58981 tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca
    59041 tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc
    59101 tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact
    59161 ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat
    59221 catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac
    59281 ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata
    59341 cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct
    59401 ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca
    59461 ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa
    59521 tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc
    59581 ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc
    59641 aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt
    59701 gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata
    59761 tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg
    59821 gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt
    59881 tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg
    59941 cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag
    60001 acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat
    60061 tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct
    60121 aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc
    60181 acctttagct gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc
    60241 tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg
    60301 atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata
    60361 ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt
    60421 ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga
    60481 taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg
    60541 atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca
    60601 ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat
    60661 acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt
    60721 tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata
    60781 atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca
    60841 tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct
    60901 tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata
    60961 gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa
    61021 cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac
    61081 atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa
    61141 ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa
    61201 ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga
    61261 acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat
    61321 gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca
    61381 gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa
    61441 atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc
    61501 acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata
    61561 aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc
    61621 atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc
    61681 accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag
    61741 tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg
    61801 acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt
    61861 aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca
    61921 gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac
    61981 gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca
    62041 gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct
    62101 ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata
    62161 gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa
    62221 aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg
    62281 ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc
    62341 ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt
    62401 actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc
    62461 atctgcggta ataagctcaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt
    62521 cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc
    62581 tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt
    62641 tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg
    62701 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
    62761 gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca
    62821 tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
    62881 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
    62941 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
    63001 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
    63061 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
    63121 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
    63181 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
    63241 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
    63301 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
    63361 tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct
    63421 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
    63481 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
    63541 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
    63601 aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct
    63661 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
    63721 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
    63781 gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct
    63841 ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg
    63901 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
    63961 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
    64021 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
    64081 tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
    64141 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
    64201 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
    64261 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca
    64321 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
    64381 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt
    64441 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
    64501 tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct
    64561 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
    64621 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat
    64681 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
    64741 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta
    64801 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
    64861 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
    64921 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
    64981 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
    65041 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
    65101 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
    65161 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
    65221 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
    65281 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
    65341 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
    65401 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
    65461 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
    65521 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
    65581 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
    65641 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
    65701 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
    65761 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
    65821 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
    65881 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
    65941 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
    66001 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
    66061 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
    66121 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
    66181 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
    66241 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
    66301 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
    66361 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
    66421 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
    66481 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
    66541 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
    66601 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
    66661 ttctttaagt ttacgtttat gtctttattt tgacaaaaaa atgtcttttc gttttggcgc
    66721 atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa
    66781 gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa
    66841 aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta
    66901 cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct
    66961 cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt
    67021 aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga
    67081 gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg
    67141 accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact
    67201 agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat
    67261 ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt
    67321 tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca
    67381 atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc
    67441 ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg
    67501 ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca
    67561 aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa
    67621 tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt
    67681 cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata
    67741 gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct
    67801 tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc
    67861 ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc
    67921 ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct
    67981 tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc
    68041 aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca
    68101 taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca
    68161 tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc
    68221 agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc
    68281 cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta
    68341 caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct
    68401 agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt
    68461 actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata
    68521 aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca
    68581 caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg
    68641 tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca
    68701 tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa
    68761 gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt
    68821 tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca
    68881 ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca
    68941 gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact
    69001 tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca
    69061 atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc
    69121 ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac
    69181 caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt
    69241 aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt
    69301 tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg
    69361 ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca
    69421 ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg
    69481 atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact
    69541 aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga
    69601 ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat
    69661 gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc
    69721 acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg
    69781 gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag
    69841 ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca
    69901 aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac
    69961 aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg
    70021 tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac
    70081 aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc
    70141 aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta
    70201 acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga
    70261 tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca
    70321 tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg
    70381 tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg
    70441 atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc
    70501 cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact
    70561 aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca
    70621 gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt
    70681 ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc
    70741 aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt
    70801 taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt
    70861 ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa
    70921 aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat
    70981 tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc
    71041 atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt
    71101 tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt
    71161 agcataatac tatctgttgc tgcaagattt tgtttgatag catcaatcaa tttattcttt
    71221 tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt
    71281 gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca
    71341 ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca
    71401 gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct
    71461 gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat
    71521 tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac
    71581 agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca
    71641 ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt
    71701 gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact
    71761 aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct
    71821 gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga
    71881 ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt
    71941 gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta
    72001 tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc
    72061 gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt
    72121 ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt
    72181 gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga
    72241 gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg
    72301 ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc
    72361 ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg
    72421 gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa
    72481 aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta
    72541 ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt
    72601 ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact
    72661 ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt
    72721 acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt
    72781 tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta
    72841 acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca
    72901 tttcgatttt attattgata tattttgctt cataatcata gaatgctacg tcttttacga
    72961 cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc
    73021 gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag
    73081 cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg
    73141 ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag
    73201 gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag
    73261 cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg
    73321 cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt
    73381 ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga
    73441 tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga
    73501 catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg
    73561 taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg
    73621 cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca
    73681 aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa
    73741 acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg
    73801 tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag
    73861 aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg
    73921 agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt
    73981 aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt
    74041 tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt
    74101 acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt
    74161 atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc
    74221 tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat
    74281 cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata
    74341 cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt
    74401 gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa
    74461 caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt
    74521 gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa
    74581 aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct
    74641 tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca
    74701 tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa
    74761 aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc
    74821 agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct
    74881 ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc
    74941 ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag
    75001 tcggttccac caagaactga caagttttct tgaacagtat tatgattctc ctattgatga
    75061 aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca
    75121 gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg
    75181 cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt
    75241 tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg
    75301 gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc
    75361 gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga
    75421 aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc
    75481 cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt
    75541 tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat
    75601 gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata
    75661 agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga
    75721 agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta
    75781 ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt
    75841 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    75901 cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg
    75961 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    76021 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca
    76081 cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    76141 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    76201 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    76261 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    76321 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    76381 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    76441 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    76501 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    76561 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa
    76621 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    76681 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    76741 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    76801 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt
    76861 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    76921 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    76981 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    77041 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    77101 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    77161 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    77221 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    77281 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    77341 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    77401 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    77461 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    77521 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    77581 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    77641 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    77701 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    77761 taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc
    77821 tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct
    77881 tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc
    77941 aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt
    78001 taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat
    78061 ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc
    78121 tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc
    78181 gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta
    78241 tttagcaaat ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa
    78301 ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt
    78361 tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg
    78421 atcggcacaa aagcttccgg ccatacttct tgtatgactg ccatcagctc atccagttca
    78481 ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct
    78541 tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga ccatggtttc
    78601 aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga
    78661 cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata
    78721 tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata
    78781 agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc
    78841 actaggccaa tacaactata taaaagccca aatccattgc caaacatcag catgcccgct
    78901 actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca
    78961 aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat
    79021 cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata
    79081 atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc
    79141 cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DACIFS010000004        78696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.004, whole genome shotgun sequence.
ACCESSION   DACIFS010000004 DACIFS010000000
VERSION     DACIFS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.004"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            194..2101
                     /locus_tag="IYA83_000253"
     CDS             194..2101
                     /locus_tag="IYA83_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ8585722.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2642..5920)
                     /locus_tag="IYA83_000254"
     CDS             complement(2642..5920)
                     /locus_tag="IYA83_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ8585723.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6191..6736)
                     /locus_tag="IYA83_000255"
     CDS             complement(6191..6736)
                     /locus_tag="IYA83_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ8585724.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(6748..7440)
                     /locus_tag="IYA83_000256"
     CDS             complement(6748..7440)
                     /locus_tag="IYA83_000256"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ8585725.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(7459..7743)
                     /locus_tag="IYA83_000257"
     CDS             complement(7459..7743)
                     /locus_tag="IYA83_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585726.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(7756..8319)
                     /locus_tag="IYA83_000258"
     CDS             complement(7756..8319)
                     /locus_tag="IYA83_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ8585727.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            8547..9209
                     /locus_tag="IYA83_000259"
     CDS             8547..9209
                     /locus_tag="IYA83_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ8585728.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            9333..10529
                     /locus_tag="IYA83_000260"
     CDS             9333..10529
                     /locus_tag="IYA83_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ8585729.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(10630..12006)
                     /locus_tag="IYA83_000261"
     CDS             complement(10630..12006)
                     /locus_tag="IYA83_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8585730.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(12043..12909)
                     /locus_tag="IYA83_000262"
     CDS             complement(12043..12909)
                     /locus_tag="IYA83_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ8585731.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(12998..14023)
                     /locus_tag="IYA83_000263"
     CDS             complement(12998..14023)
                     /locus_tag="IYA83_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8585732.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(14041..14715)
                     /locus_tag="IYA83_000264"
     CDS             complement(14041..14715)
                     /locus_tag="IYA83_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8585733.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(14899..15792)
                     /locus_tag="IYA83_000265"
     CDS             complement(14899..15792)
                     /locus_tag="IYA83_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ8585734.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(15866..17563)
                     /locus_tag="IYA83_000266"
     CDS             complement(15866..17563)
                     /locus_tag="IYA83_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ8585735.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(17556..18869)
                     /locus_tag="IYA83_000267"
     CDS             complement(17556..18869)
                     /locus_tag="IYA83_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8585736.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(18869..22906)
                     /locus_tag="IYA83_000268"
     CDS             complement(18869..22906)
                     /locus_tag="IYA83_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ8585737.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(22919..23377)
                     /locus_tag="IYA83_000269"
     CDS             complement(22919..23377)
                     /locus_tag="IYA83_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8585738.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(23675..24193)
                     /locus_tag="IYA83_000270"
     CDS             complement(23675..24193)
                     /locus_tag="IYA83_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ8585739.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(24195..24812)
                     /locus_tag="IYA83_000271"
     CDS             complement(24195..24812)
                     /locus_tag="IYA83_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ8585740.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(24809..25663)
                     /locus_tag="IYA83_000272"
     CDS             complement(24809..25663)
                     /locus_tag="IYA83_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8585741.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(25660..26571)
                     /locus_tag="IYA83_000273"
     CDS             complement(25660..26571)
                     /locus_tag="IYA83_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8585742.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(26612..27946)
                     /locus_tag="IYA83_000274"
     CDS             complement(26612..27946)
                     /locus_tag="IYA83_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8585743.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            28154..28984
                     /locus_tag="IYA83_000275"
     CDS             28154..28984
                     /locus_tag="IYA83_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ8585744.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(29228..29477)
                     /locus_tag="IYA83_000276"
                     /pseudo
     CDS             complement(29228..29477)
                     /locus_tag="IYA83_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29668..29862)
                     /locus_tag="IYA83_000277"
     CDS             complement(29668..29862)
                     /locus_tag="IYA83_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585745.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(30367..30552)
                     /locus_tag="IYA83_000278"
     CDS             complement(30367..30552)
                     /locus_tag="IYA83_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585746.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(31402..31743)
                     /locus_tag="IYA83_000279"
     CDS             complement(31402..31743)
                     /locus_tag="IYA83_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ8585747.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(31743..31973)
                     /locus_tag="IYA83_000280"
     CDS             complement(31743..31973)
                     /locus_tag="IYA83_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8585748.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            32260..32598
                     /locus_tag="IYA83_000281"
     CDS             32260..32598
                     /locus_tag="IYA83_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585749.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(32803..33042)
                     /locus_tag="IYA83_000282"
     CDS             complement(32803..33042)
                     /locus_tag="IYA83_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ8585750.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(33018..33365)
                     /locus_tag="IYA83_000283"
     CDS             complement(33018..33365)
                     /locus_tag="IYA83_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ8585751.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(34097..34678)
                     /locus_tag="IYA83_000284"
     CDS             complement(34097..34678)
                     /locus_tag="IYA83_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8585752.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(34861..35487)
                     /locus_tag="IYA83_000285"
     CDS             complement(34861..35487)
                     /locus_tag="IYA83_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ8585753.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(35509..36840)
                     /locus_tag="IYA83_000286"
     CDS             complement(35509..36840)
                     /locus_tag="IYA83_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ8585754.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(36995..37396)
                     /locus_tag="IYA83_000287"
     CDS             complement(36995..37396)
                     /locus_tag="IYA83_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ8585755.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(37482..37688)
                     /locus_tag="IYA83_000288"
     CDS             complement(37482..37688)
                     /locus_tag="IYA83_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585756.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(37783..39285)
                     /locus_tag="IYA83_000289"
     CDS             complement(37783..39285)
                     /locus_tag="IYA83_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ8585757.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(39303..39758)
                     /gene="argR"
                     /locus_tag="IYA83_000290"
     CDS             complement(39303..39758)
                     /gene="argR"
                     /locus_tag="IYA83_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ8585758.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(39856..40473)
                     /locus_tag="IYA83_000291"
     CDS             complement(39856..40473)
                     /locus_tag="IYA83_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585759.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            40694..42067
                     /locus_tag="IYA83_000292"
     CDS             40694..42067
                     /locus_tag="IYA83_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ8585760.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            42142..43254
                     /locus_tag="IYA83_000293"
     CDS             42142..43254
                     /locus_tag="IYA83_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ8585761.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(43326..44963)
                     /locus_tag="IYA83_000294"
     CDS             complement(43326..44963)
                     /locus_tag="IYA83_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ8585762.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(45131..45739)
                     /locus_tag="IYA83_000295"
     CDS             complement(45131..45739)
                     /locus_tag="IYA83_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ8585763.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(46123..47274)
                     /gene="queG"
                     /locus_tag="IYA83_000296"
     CDS             complement(46123..47274)
                     /gene="queG"
                     /locus_tag="IYA83_000296"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ8585764.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(47293..48045)
                     /locus_tag="IYA83_000297"
     CDS             complement(47293..48045)
                     /locus_tag="IYA83_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585765.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(48186..49136)
                     /locus_tag="IYA83_000298"
     CDS             complement(48186..49136)
                     /locus_tag="IYA83_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ8585766.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(49137..49976)
                     /locus_tag="IYA83_000299"
     CDS             complement(49137..49976)
                     /locus_tag="IYA83_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ8585767.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(49973..50719)
                     /locus_tag="IYA83_000300"
     CDS             complement(49973..50719)
                     /locus_tag="IYA83_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585768.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            50957..52327
                     /locus_tag="IYA83_000301"
     CDS             50957..52327
                     /locus_tag="IYA83_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8585769.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(52382..53278)
                     /locus_tag="IYA83_000302"
     CDS             complement(52382..53278)
                     /locus_tag="IYA83_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ8585770.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(53559..54236)
                     /locus_tag="IYA83_000303"
     CDS             complement(53559..54236)
                     /locus_tag="IYA83_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ8585771.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(54327..55232)
                     /locus_tag="IYA83_000304"
     CDS             complement(54327..55232)
                     /locus_tag="IYA83_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ8585772.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            55407..56861
                     /locus_tag="IYA83_000305"
     CDS             55407..56861
                     /locus_tag="IYA83_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8585773.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            56877..57908
                     /locus_tag="IYA83_000306"
     CDS             56877..57908
                     /locus_tag="IYA83_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ8585774.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            57915..59102
                     /locus_tag="IYA83_000307"
     CDS             57915..59102
                     /locus_tag="IYA83_000307"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ8585775.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            59258..60640
                     /locus_tag="IYA83_000308"
     CDS             59258..60640
                     /locus_tag="IYA83_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ8585776.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            60767..60997
                     /locus_tag="IYA83_000309"
     CDS             60767..60997
                     /locus_tag="IYA83_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585777.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(61197..61865)
                     /locus_tag="IYA83_000310"
     CDS             complement(61197..61865)
                     /locus_tag="IYA83_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ8585778.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(62054..62851)
                     /locus_tag="IYA83_000311"
                     /pseudo
     CDS             complement(62054..62851)
                     /locus_tag="IYA83_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(63132..64511)
                     /locus_tag="IYA83_000312"
     CDS             complement(63132..64511)
                     /locus_tag="IYA83_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ8585779.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(64686..65855)
                     /locus_tag="IYA83_000313"
     CDS             complement(64686..65855)
                     /locus_tag="IYA83_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ8585780.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(65830..66981)
                     /locus_tag="IYA83_000314"
     CDS             complement(65830..66981)
                     /locus_tag="IYA83_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ8585781.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(66941..68302)
                     /locus_tag="IYA83_000315"
     CDS             complement(66941..68302)
                     /locus_tag="IYA83_000315"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ8585782.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(68299..69300)
                     /locus_tag="IYA83_000316"
     CDS             complement(68299..69300)
                     /locus_tag="IYA83_000316"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ8585783.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(69316..70011)
                     /gene="dapD"
                     /locus_tag="IYA83_000317"
     CDS             complement(69316..70011)
                     /gene="dapD"
                     /locus_tag="IYA83_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ8585784.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(70017..70733)
                     /locus_tag="IYA83_000318"
     CDS             complement(70017..70733)
                     /locus_tag="IYA83_000318"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ8585785.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(70730..71611)
                     /locus_tag="IYA83_000319"
     CDS             complement(70730..71611)
                     /locus_tag="IYA83_000319"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ8585786.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(71614..72414)
                     /gene="dapF"
                     /locus_tag="IYA83_000320"
     CDS             complement(71614..72414)
                     /gene="dapF"
                     /locus_tag="IYA83_000320"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ8585787.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(72401..73660)
                     /gene="lysA"
                     /locus_tag="IYA83_000321"
     CDS             complement(72401..73660)
                     /gene="lysA"
                     /locus_tag="IYA83_000321"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ8585788.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(74146..74709)
                     /gene="efp"
                     /locus_tag="IYA83_000322"
     CDS             complement(74146..74709)
                     /gene="efp"
                     /locus_tag="IYA83_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ8585789.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            75137..76054
                     /locus_tag="IYA83_000323"
     CDS             75137..76054
                     /locus_tag="IYA83_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ8585790.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            76067..77212
                     /locus_tag="IYA83_000324"
     CDS             76067..77212
                     /locus_tag="IYA83_000324"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ8585791.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            77383..77841
                     /locus_tag="IYA83_000325"
     CDS             77383..77841
                     /locus_tag="IYA83_000325"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ8585792.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            78000..78470
                     /locus_tag="IYA83_000326"
     CDS             78000..78470
                     /locus_tag="IYA83_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ8585793.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(78523..78638)
                     /gene="rrf"
                     /locus_tag="IYA83_000327"
     rRNA            complement(78523..78638)
                     /gene="rrf"
                     /locus_tag="IYA83_000327"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        22586 a        16470 c        14000 g        25640 t
ORIGIN      
        1 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
       61 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
      121 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
      181 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
      241 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
      301 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
      361 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
      421 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
      481 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
      541 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
      601 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
      661 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
      721 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
      781 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
      841 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
      901 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
      961 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
     1021 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     1081 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     1141 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     1201 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     1261 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     1321 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     1381 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     1441 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     1501 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     1561 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     1621 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     1681 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     1741 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     1801 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     1861 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     1921 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     1981 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     2041 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     2101 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     2161 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     2221 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     2281 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     2341 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     2401 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     2461 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     2521 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     2581 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     2641 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     2701 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     2761 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     2821 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     2881 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     2941 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     3001 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     3061 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     3121 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     3181 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     3241 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     3301 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     3361 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     3421 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     3481 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     3541 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     3601 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     3661 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     3721 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     3781 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     3841 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     3901 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     3961 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     4021 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     4081 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     4141 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     4201 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     4261 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     4321 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     4381 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     4441 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     4501 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     4561 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     4621 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     4681 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     4741 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     4801 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     4861 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     4921 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     4981 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     5041 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     5101 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     5161 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     5221 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     5281 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     5341 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     5401 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     5461 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     5521 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     5581 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     5641 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     5701 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     5761 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     5821 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     5881 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     5941 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     6001 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
     6061 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
     6121 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
     6181 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
     6241 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
     6301 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
     6361 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
     6421 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
     6481 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
     6541 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
     6601 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
     6661 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
     6721 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
     6781 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
     6841 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
     6901 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
     6961 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
     7021 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
     7081 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
     7141 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
     7201 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     7261 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     7321 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     7381 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     7441 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     7501 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     7561 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     7621 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     7681 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     7741 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     7801 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     7861 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     7921 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     7981 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     8041 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     8101 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     8161 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
     8221 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     8281 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     8341 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     8401 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     8461 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     8521 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     8581 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     8641 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     8701 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     8761 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     8821 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     8881 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     8941 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     9001 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     9061 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     9121 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     9181 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     9241 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     9301 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     9361 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     9421 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     9481 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     9541 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     9601 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     9661 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     9721 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     9781 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     9841 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     9901 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     9961 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
    10021 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
    10081 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
    10141 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
    10201 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
    10261 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
    10321 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
    10381 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
    10441 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
    10501 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
    10561 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
    10621 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
    10681 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
    10741 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
    10801 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
    10861 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
    10921 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
    10981 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
    11041 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
    11101 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
    11161 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
    11221 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
    11281 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
    11341 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
    11401 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
    11461 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
    11521 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
    11581 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
    11641 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
    11701 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
    11761 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
    11821 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
    11881 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
    11941 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
    12001 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
    12061 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
    12121 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
    12181 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
    12241 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
    12301 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
    12361 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
    12421 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
    12481 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
    12541 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
    12601 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
    12661 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
    12721 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
    12781 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
    12841 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
    12901 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
    12961 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
    13021 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
    13081 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
    13141 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
    13201 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
    13261 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
    13321 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
    13381 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
    13441 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
    13501 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
    13561 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
    13621 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
    13681 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
    13741 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
    13801 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
    13861 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
    13921 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
    13981 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
    14041 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
    14101 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
    14161 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
    14221 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
    14281 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
    14341 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
    14401 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
    14461 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
    14521 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
    14581 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
    14641 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
    14701 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
    14761 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
    14821 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
    14881 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
    14941 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
    15001 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
    15061 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
    15121 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
    15181 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
    15241 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
    15301 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
    15361 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
    15421 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
    15481 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
    15541 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
    15601 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
    15661 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
    15721 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
    15781 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
    15841 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
    15901 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
    15961 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
    16021 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    16081 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    16141 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    16201 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    16261 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    16321 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    16381 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    16441 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    16501 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    16561 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    16621 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    16681 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    16741 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    16801 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    16861 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    16921 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    16981 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    17041 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    17101 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    17161 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    17221 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    17281 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    17341 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    17401 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    17461 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    17521 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    17581 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    17641 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    17701 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    17761 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    17821 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    17881 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    17941 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    18001 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    18061 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    18121 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    18181 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    18241 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    18301 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    18361 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    18421 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    18481 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    18541 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    18601 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    18661 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    18721 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    18781 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    18841 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    18901 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    18961 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    19021 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    19081 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    19141 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    19201 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    19261 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    19321 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    19381 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    19441 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    19501 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    19561 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    19621 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    19681 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    19741 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    19801 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    19861 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    19921 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    19981 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    20041 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    20101 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    20161 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    20221 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    20281 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    20341 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    20401 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    20461 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    20521 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    20581 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    20641 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    20701 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    20761 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    20821 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    20881 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    20941 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    21001 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    21061 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    21121 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    21181 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    21241 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    21301 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    21361 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    21421 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    21481 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    21541 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    21601 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    21661 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    21721 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    21781 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    21841 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    21901 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    21961 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    22021 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    22081 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    22141 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    22201 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    22261 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    22321 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    22381 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    22441 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    22501 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    22561 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    22621 tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag
    22681 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    22741 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    22801 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    22861 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    22921 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    22981 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    23041 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    23101 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    23161 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    23221 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    23281 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    23341 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    23401 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    23461 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    23521 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    23581 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    23641 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    23701 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    23761 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    23821 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    23881 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    23941 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    24001 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    24061 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    24121 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    24181 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    24241 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    24301 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    24361 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    24421 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    24481 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    24541 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    24601 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    24661 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    24721 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    24781 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    24841 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    24901 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    24961 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    25021 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    25081 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    25141 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    25201 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    25261 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    25321 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    25381 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    25441 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    25501 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    25561 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    25621 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    25681 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    25741 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    25801 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    25861 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    25921 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    25981 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    26041 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    26101 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    26161 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    26221 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    26281 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    26341 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    26401 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    26461 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    26521 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    26581 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    26641 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    26701 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    26761 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    26821 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    26881 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    26941 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    27001 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    27061 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    27121 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    27181 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    27241 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    27301 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    27361 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    27421 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    27481 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    27541 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    27601 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    27661 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    27721 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    27781 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    27841 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    27901 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    27961 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    28021 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    28081 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    28141 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    28201 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    28261 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    28321 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    28381 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    28441 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagctggctt
    28501 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    28561 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    28621 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    28681 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    28741 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    28801 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    28861 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    28921 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    28981 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    29041 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    29101 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    29161 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    29221 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    29281 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    29341 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    29401 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    29461 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    29521 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    29581 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    29641 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    29701 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    29761 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    29821 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    29881 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    29941 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    30001 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    30061 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    30121 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    30181 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    30241 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    30301 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    30361 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    30421 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    30481 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    30541 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    30601 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
    30661 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    30721 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    30781 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    30841 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    30901 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    30961 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    31021 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    31081 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    31141 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    31201 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    31261 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    31321 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    31381 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    31441 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    31501 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    31561 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    31621 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    31681 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    31741 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    31801 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    31861 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    31921 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    31981 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
    32041 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
    32101 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
    32161 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
    32221 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
    32281 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
    32341 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
    32401 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
    32461 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
    32521 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
    32581 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
    32641 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
    32701 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
    32761 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
    32821 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
    32881 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
    32941 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
    33001 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
    33061 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
    33121 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
    33181 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
    33241 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
    33301 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
    33361 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
    33421 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
    33481 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
    33541 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
    33601 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
    33661 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
    33721 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    33781 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
    33841 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
    33901 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
    33961 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
    34021 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    34081 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
    34141 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
    34201 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
    34261 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
    34321 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
    34381 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
    34441 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
    34501 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
    34561 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
    34621 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
    34681 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
    34741 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
    34801 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
    34861 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
    34921 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
    34981 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
    35041 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
    35101 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
    35161 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
    35221 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
    35281 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
    35341 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
    35401 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
    35461 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
    35521 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
    35581 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
    35641 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
    35701 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
    35761 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
    35821 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
    35881 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
    35941 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
    36001 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
    36061 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
    36121 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
    36181 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
    36241 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
    36301 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
    36361 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
    36421 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
    36481 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
    36541 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
    36601 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
    36661 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
    36721 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
    36781 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
    36841 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
    36901 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
    36961 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
    37021 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
    37081 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
    37141 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
    37201 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
    37261 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
    37321 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
    37381 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
    37441 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
    37501 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
    37561 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
    37621 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
    37681 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
    37741 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
    37801 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
    37861 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
    37921 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
    37981 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
    38041 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
    38101 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
    38161 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
    38221 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
    38281 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
    38341 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
    38401 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
    38461 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
    38521 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
    38581 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
    38641 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
    38701 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
    38761 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
    38821 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
    38881 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
    38941 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
    39001 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
    39061 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
    39121 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
    39181 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
    39241 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
    39301 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
    39361 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
    39421 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
    39481 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
    39541 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
    39601 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
    39661 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
    39721 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
    39781 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
    39841 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
    39901 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
    39961 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
    40021 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
    40081 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
    40141 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
    40201 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
    40261 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
    40321 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
    40381 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
    40441 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
    40501 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    40561 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    40621 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    40681 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    40741 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    40801 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    40861 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    40921 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    40981 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    41041 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    41101 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    41161 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
    41221 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    41281 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    41341 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    41401 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    41461 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    41521 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    41581 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    41641 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    41701 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    41761 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    41821 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    41881 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    41941 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    42001 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    42061 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    42121 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    42181 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    42241 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    42301 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    42361 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    42421 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    42481 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    42541 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    42601 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    42661 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    42721 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    42781 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    42841 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    42901 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    42961 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    43021 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    43081 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    43141 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    43201 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    43261 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    43321 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    43381 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    43441 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    43501 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    43561 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    43621 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    43681 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    43741 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    43801 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    43861 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    43921 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    43981 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    44041 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    44101 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    44161 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    44221 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    44281 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    44341 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    44401 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    44461 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    44521 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    44581 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    44641 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    44701 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    44761 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    44821 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    44881 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    44941 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    45001 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    45061 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    45121 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    45181 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    45241 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    45301 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    45361 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    45421 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    45481 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    45541 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    45601 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    45661 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    45721 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    45781 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    45841 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    45901 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    45961 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    46021 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    46081 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    46141 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    46201 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    46261 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    46321 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    46381 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    46441 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    46501 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    46561 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    46621 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    46681 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    46741 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    46801 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    46861 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    46921 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    46981 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    47041 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    47101 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    47161 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    47221 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    47281 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    47341 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    47401 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    47461 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    47521 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    47581 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    47641 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    47701 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    47761 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    47821 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    47881 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    47941 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    48001 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    48061 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    48121 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    48181 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    48241 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    48301 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    48361 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    48421 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    48481 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    48541 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    48601 tatgcttctg catttttttt atagagtgtc gcattttctg catctttttc agagaatata
    48661 cgtgcgattt cctccacata ttttattccg ttagataggt ctagccaagc atggggatct
    48721 gcttttgtat gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca
    48781 aaataatctt ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta
    48841 cctgtttcaa gattcaaacc gttgtataaa ataacatctg catcgcttgc ctttttgatg
    48901 tcttctggta gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata
    48961 tctatttgat ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg
    49021 actgacaact tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg
    49081 gctaatacca agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat
    49141 ctgcttcctt tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa
    49201 aatgcggtac atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa
    49261 aacacaccga caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt
    49321 aaatggttcg ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag
    49381 attgttccta ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga
    49441 taatggatca gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg
    49501 atcttcaatt ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct
    49561 gtcagcaaca aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa
    49621 tctgtcgcac tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag
    49681 acaattccaa tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact
    49741 gctgctgtta gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa
    49801 gaaatggcta cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct
    49861 cgtaaaataa taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc
    49921 aaagcgtatt gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt
    49981 tctctcctcc ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg
    50041 atgtaaagac ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct
    50101 catcgaaata ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg
    50161 aattcttcaa ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta
    50221 cgaatggttc atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa
    50281 ctctctgaag ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc
    50341 ctactttttt caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa
    50401 ttcgtaaaga tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa
    50461 gatccaattc acttcgctgt tccacgtagg ctattttttt acggactgag tttaccgact
    50521 gtccagaaaa ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa
    50581 atgttgattt tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg
    50641 tcaagttgat agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta
    50701 caatggccgt acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa
    50761 atatagttta actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga
    50821 attttcctcg cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta
    50881 acctaataat gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa
    50941 cgaaaggagt ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat
    51001 ccaaatgatc gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac
    51061 catacgttgg acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta
    51121 tttgattatg cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc
    51181 aaattacgcg agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat
    51241 ttttcaatat attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta
    51301 ttggtggcct gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct
    51361 ggcaaatctt atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa
    51421 agtcataacg atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg
    51481 taatcatggt caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg
    51541 aggcgtcaaa ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga
    51601 actgatcgga atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt
    51661 ccagtccact atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag
    51721 tatctatccg tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa
    51781 agtcggcatt acatttgctg caggactgat taattttgtc gtgattacag cggcattatc
    51841 aggttgcaat tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg
    51901 aaagatgtct aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt
    51961 agcaatctca ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat
    52021 caaccagtca gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt
    52081 tccttgggta gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat
    52141 gcatgatcat ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt
    52201 tttagctatc gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat
    52261 tggactcgtt ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg
    52321 aaaataaaaa aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt
    52381 atcaattcaa ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat
    52441 tcagcgacaa gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt
    52501 ctccttccaa aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag
    52561 aggaatctgt tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg
    52621 ctgtctcaga atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg
    52681 cgctagtctg caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata
    52741 attgattgat caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg
    52801 cacgtaattt tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag
    52861 caactgctga taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt
    52921 caatcaatgc aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa
    52981 gttgctctgg caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag
    53041 ttgatgactc acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg
    53101 tacttttcgc ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta
    53161 tgatcaaaaa cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt
    53221 tttgctgtct tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt
    53281 ttttcaccac caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag
    53341 caaataattg tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt
    53401 gagatgattc caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat
    53461 ctcagccatc agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc
    53521 gcttaattca tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt
    53581 tcttgcagtg taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg
    53641 atatatgctt gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga
    53701 ataaaccagt caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga
    53761 taagcaatta gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg
    53821 aatggcggaa gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat
    53881 tcggaaatcg tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt
    53941 gtcaatacga ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta
    54001 tcttgccaaa tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg
    54061 atccgatcat gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga
    54121 taacttgatt ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat
    54181 tgctgattta aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct
    54241 tttcctcctt tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc
    54301 gatgcttgtc attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc
    54361 aactgttttt tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta
    54421 cgatagttcg tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga
    54481 ctattgaacc ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga
    54541 aacgcccctc gattgatccg taccccgatc aaatattcct tgatactggt acccatatga
    54601 tttttgaata ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca
    54661 acagacaatt tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag
    54721 gaatgaacct tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa
    54781 aaacggtagg ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc
    54841 atgcattcta aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg
    54901 tcttctctga aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc
    54961 cagcaataca tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga
    55021 atcaagaaca gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct
    55081 ttgccttcca tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga
    55141 aacggcatcg ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga
    55201 tgttcctttt ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata
    55261 atgcaacaaa aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt
    55321 taagtttaag tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata
    55381 tattgaacag gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg
    55441 ctttaggcgc ttttggacac gacacattct atgttacatt aagcacttat tttatggtat
    55501 tcgtgacaag ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta
    55561 cagttacaag cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg
    55621 gtggcatcat cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg
    55681 gcggcgttgt tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta
    55741 ctcataatac gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt
    55801 tttattcatt taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa
    55861 aggaacgtga aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta
    55921 ctactctagt cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata
    55981 ttgaaggcgc ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta
    56041 ctgcgattat cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg
    56101 aaaaagctgg gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc
    56161 ttgcccttgc ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct
    56221 acttcttcaa atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg
    56281 ccttacttgg tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc
    56341 gctacgttta ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg
    56401 caggttcaag tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat
    56461 tgattttctt atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa
    56521 atggtatccg taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg
    56581 gtgctttgtc taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg
    56641 ctactgctgc tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg
    56701 ctcctgctgc tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag
    56761 agaaaaaaca tgcacaaatc gttgagaaat tggaaaacaa attggctcca gctgatacaa
    56821 tggaagatga acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga
    56881 agattacaga aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca
    56941 agctagcact ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc
    57001 gagaattagt tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg
    57061 gtgcttctat cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa
    57121 aaacttacca attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg
    57181 gatttgaatt aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca
    57241 tttttactac tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa
    57301 tccgctatac tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc
    57361 aagatacttt attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc
    57421 agtcaatcga tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag
    57481 atagtattcc tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac
    57541 ctaaaaaatt agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa
    57601 tcgatcatcc atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta
    57661 gccccgataa aagaatccaa gtagatattg ctacagatgc ttctagtgtg gttattttca
    57721 ctgcaaattt cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg
    57781 gcatcacatt tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga
    57841 ttcatttaaa agccggatca gtatttgaaa cagtaacaga attcaaaatc aaaacaagaa
    57901 aggaataact catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg
    57961 gacctaaaaa aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca
    58021 cagattacaa tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag
    58081 cagcacctcg agacgataat aaaatattag tttattccac aaactttgac gatatcggtg
    58141 tcattagttt tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg
    58201 taaaaggaat ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt
    58261 tattagtgga aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat
    58321 tattagtagg tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag
    58381 tccaaacagg taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc
    58441 agtttgccat cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga
    58501 aatacgaaat ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata
    58561 agcggcggga attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct
    58621 tggcacgttt gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat
    58681 ttgaatcaac tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg
    58741 tttctgaaaa tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag
    58801 catttggtca actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg
    58861 ggatcgaatt agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac
    58921 gaatgacagg agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac
    58981 ctgccttcaa aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat
    59041 tttatgttgc acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt
    59101 gataaaaaaa tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt
    59161 tccaaatgtg agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga
    59221 gaacgtgtgt tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt
    59281 atttgtttat gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta
    59341 cgcctccgta gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat
    59401 gtcctatccg tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc
    59461 tgcagcaaaa aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc
    59521 ctatccaagt gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag
    59581 agaaatcaat tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt
    59641 cgatgaaagc ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta
    59701 cgacatggct aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat
    59761 aggaattggc gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg
    59821 aaagcaaatg tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc
    59881 agatatcact gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg
    59941 tatacattct gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg
    60001 ggtcatcggt actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca
    60061 agaatataca ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac
    60121 aagacgagaa gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt
    60181 gagacgatct gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta
    60241 tcttgatcca aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc
    60301 tagcagagat ctagcagatg ctttattgac cattttccac cagcattacc actaccagga
    60361 tattcggcgt gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa
    60421 cctttttgaa gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac
    60481 gattagaaaa aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg
    60541 acgcgccatc gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga
    60601 aggtggaaaa gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc
    60661 ggccatttgg attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca
    60721 aaaaaatgaa gaagaagcac gcaaacagcc tcttcctaaa caacaaatga cattgcaaga
    60781 aatcgatagt atactgacag aagccttttt atctcgtaaa gcagtaatca ttcagctcaa
    60841 tttaaaagat gaatttggaa gactcttgga tgaggtcgaa gggcacttca ttggtgaagc
    60901 ctatgaagat tactttatga ttgaccatca gccgatattg tgggaagacg tacggtatat
    60961 tgagttaaag gaaacaaaaa aatggtttgt gacatgagag gttgtgacag aagtgatcaa
    61021 cttcaaaaaa taagaaagga tttttcgaaa attgcttctt aaatttttgt ggaattttgg
    61081 cttatttttc gaagaagcaa cttctggaac aacgtatatt agatatcaga ggttgtgaaa
    61141 aagtcgctta gttttgagta acaagccaag gcgctgattt cacaacctct gtctatctat
    61201 ccatctattc ggtaagattc atgcatgacg tcttctttat ttagtatgta gagccctttt
    61261 tcttctgacc attcaacata aatcagctgt tttacatctg catagccttc tttcttcaag
    61321 ttctccataa gccatgcttc agtcaattga ttggactgaa gcactttatg ctgaatttgc
    61381 ccttcatcta caagcaggat cgataaagct ttgtcttcat ctgatttttt caatacactc
    61441 atttgaccgg cattctctag gataacatgt ttggcatttt caagaaaaaa gcattcttgc
    61501 tgtcttaaga ttgctcgcaa ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg
    61561 ttgatgacgc cgtctttgat tagaacagat ggctcgcctt tgacccaacg attgaccttt
    61621 tcattttttt ggacgatacg ttcgatacca taaatcatta ctgcccaaag cgcaatggca
    61681 aataggacat gccctacatg gacattgtca tcataggttg attcttctaa gatcccacct
    61741 aaaacaagcg tatagactaa atcaaacggt gtgatttctg acatggattt cttcccaagc
    61801 aaacgtacca ctaataataa accgatcaac ccgaacacca gtttcattgt aattgaaaaa
    61861 atcatttttt ctccccctaa ctatctgtta attaaattat atatgctttc tcacgaacac
    61921 gcatggaata tacaaggaag aaatgtatcc ttttactaaa aaaaggactg tgacactgta
    61981 gtgaccccca aaagttagac ttttttggag tggagaaatc cactcctttt ttttatgctc
    62041 taaaatgata tttttacaca accgttacgg ttgttttacg aaattggacg agactttgcc
    62101 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
    62161 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
    62221 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
    62281 agattttgta ctccttcaat ttgtttctat aagctttcat ttgatacgcc caaccttgat
    62341 ctgagtgaaa cgtacgacga tagggacaat cgttagtggt ctggatagct tcctccaaac
    62401 cttccattac cgctaatgca ttgggctttt cagagatacg ataggataaa atctcactat
    62461 tgaacatatc cataaaagga tctagataca atttcttttg acggataatg cctttggcat
    62521 ccacttcatg gtatttaaat tcagttgtat ccgtcgtgat tttctgatgg cagatactgg
    62581 tgtagaaacg acgatggatc cgatttttgg ctactgctcc aacttttcct cgataagaat
    62641 tgtagcgacg tgatttacgc gtgtatgccg ttacttttat tcctagcttc ttgatcaagc
    62701 gctgaacttt ctttttattg atagagaaac cgcggtttct tagttcattg gtcatccgta
    62761 aacagccata atccttgtgc ttctcccgaa ttttaagcat ttccgcttca atctcttgat
    62821 ctggattttt acggtccaat cgcctttgcc atccaaataa tcatgaacga tctttaattt
    62881 aagttcaaaa gtatatttaa ccataaaaaa taccgacctc caaaagttag attttttggt
    62941 ctaacttttg ggggtcggta cacaaatcgt gtcacagtcc cttttttgaa aataaacggt
    63001 gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata agaagctgtt
    63061 tttacttggc tgttcttatt acttgatgca aggcagcttt ttcacaacct cttttcaaat
    63121 atttcgtttt cttattctac aaatggttct tctcctaacg cttgttgagc ggctagattc
    63181 aacatgctcc attgtcggtc aaagcctggc tggaagaaga agtcagcttc tgctagttct
    63241 tctaaagtca gtttgttttg gatcgctagt gccaatacat ttccttgacc tgtaacatca
    63301 taggtagata atactgctcc tcctagcaat tgatgggtca ttggatccga gaataattgg
    63361 atggacactt ttggatttcc ttgttcttct ggtacatatt ttggtcgtaa cgtatcttca
    63421 taataagaca ctcgtacatc tactcctgct tttgctgctg taaagctatt caatccgctt
    63481 gctgcaaaat gatagtcaaa gacacttaat gcagaggaac ctacgactcc gccgaacggt
    63541 ttgtttggta cttcttcaaa aagatgtttt actacataac gtgcttctct acgtgcaaca
    63601 gtcgctaaag cgatcggcat ttttttgcgt gctgggatag aataagcaag tgtagcatct
    63661 cctactgcat acacatctgg aagattcgtt tgcaagtatt catttgtttg gatccagcca
    63721 cgttcatcta agtcgacgat cccttttaac cattctgtat ttgccttcac accggcagct
    63781 tgaatgacta gatctgctgg gatttctttt tcacttgttt tgattgcctc aacttttccg
    63841 ttaccagtaa aagcttcgat ttttgcattt gtcaaaactt caataccgtt ttcatttagg
    63901 tgttgttcta aaatatctgt catctcagca tctaaataag tgcctaaagg tctatcgatc
    63961 acatcaagca atacgacttc tttcccagct tttcggcttg cttctgctgc ttcgatccca
    64021 atgtaacccg cacctacgac agcgattttt ttaatagccg gatcatttaa tttttctttg
    64081 attttggttg cccaattgta tccacgcatc aagtagacat tttctagatc tgctcctggt
    64141 actggcagat tgtttggttt gacaccagaa ctcaaaatca atttgtcata aggatattct
    64201 tttgtttcgt tatctgatgc gctagtaacc gttactgttt ttttatcggc atcgatcgtt
    64261 tttacttcgt gattatttaa aatatgaaca ttggattgcg gaaagttttc tggtctgaaa
    64321 ttacgtacat catttacatc tgttaccttg tcttctaaat acagctccat accacatgac
    64381 atgaaggaaa tatagtctcc tgcttcaaat agcgtgatat ctacattttc accgtatcgt
    64441 gtcaatagtt ccaagatcga ttgatgccct ccatgagatg cacctacaat gacaactttg
    64501 attttttcca ttgatgaaac ctccttgaat attcttttaa aatcattcta aacaatttaa
    64561 acttaacatt acattagtta tgaatcaaaa gaaacgcgtt catttgaact aaatttaaaa
    64621 aacaatcgtc tccttgcaga atataccgca aaattaacga ttgttttttt gataaaatct
    64681 atgaatcact gttctgttaa aaagtctttg atccgtttgc aggcctcatg taatttttcc
    64741 atgctgctag cataagaaat ccggatataa ccttctcctt ctgggccaaa tgcaattcct
    64801 gggatacacg caacttttgc ctgttccgct aattctacac aaaactgcca agagtcagtt
    64861 tgaatggcag ctgggagttt gcaaaacaag taaaatgcac cgtttggctg ggtcacttca
    64921 aatcctaaag gcgccaattg ctggaccaga tagtctcttc gtgtttgata ttgctctttc
    64981 atattctcgc tatctttcac cccatttatt agcgcttcag ccgcagcttt ttgcgacatc
    65041 gttgtagcgg cagtcaccag atattgatgg actttaatta gttcgtcaat aagggctttt
    65101 tgagcaaaaa taaagcccac tcgccaacca gtcatcgcat gtgacttgga taacccgctt
    65161 atcacgattg tttgttctgg aagataagta gctatggaca tatgcgttcc actataagtc
    65221 aattcagcat atacttcatc acttataaca aataccggat atttttttaa aacctcagcg
    65281 attgctttca attgttttgc agacaatgtc gtcccagtag gattgttagg atagtttaaa
    65341 atgactgctt tgacagctga tccatattgt tcaaatgctc gttcaaattg ttccggctga
    65401 cagacaaacc ctgtgtcaga agtatctatc gggatcaatt cagcaccagc tagttccact
    65461 aatggctgat atcctgaata agcaggcgct ggaataagaa ccttgtctcc ttcttccaaa
    65521 acagataaca acgcagaggc aatcgcttcc gttgcaccaa ctgtcgtcaa aacttccgtt
    65581 tgcggatcat aagttaatcc ataacgctgt tgctgaaata tacaagccgc ttcccgcagt
    65641 tcctctaatc ctctcattcc ggtatagtga gaaaagtctt cttcaatcgc tgctattcct
    65701 gcttgcttga catgttcagg agttggaaaa tccggttctc ccagggtgag tttgatcaca
    65761 tctggtatcg acgatatttg ctgatcgaat tgtcgtattt ttgatacttc tattctagaa
    65821 agcctaggac taaatcgatt ggataaattc atcattattt actcgctcct cttttgaact
    65881 gctatcgatt tttacaagaa gattttctgt aaaaagaatc gttttgtacc attctacacc
    65941 attttctaaa acatccatat caaaatcgaa cgtcggatca tgtaaagcag gggtatcacc
    66001 gacacctaaa aagaaaaata cacatggata ttgttgcgta tatatgccaa aatcttcggc
    66061 ttgaagaact ggagcatcta attcatgtag tttgtttgaa tctaacagtg ctgcatataa
    66121 gcctctatgg ttgattacag ctggatagcc gtcattatag cgaaaagaaa tagtagctgt
    66181 agagtgaaac tgttctgcta aatggtctaa tcccctcttc aaataatttt gcatatctga
    66241 actgtaagaa cggatacttc cggatacaat aacttctcca gctaaaacat ttcggatcgt
    66301 tccaccttgg atttggccaa atttcattag atgattccac tctgccggta attgccggtc
    66361 aaaatcataa acattcttca aaaatcgtac agctgcctcc agactatcga ttcctttttc
    66421 actagaagca atatgtgcag actggccttt aatgataatg tctgtttcac tgctttgtgc
    66481 catcaaaggt cctggtcgag agaaaacttc tcccttagct agtccaggcc atagatgaaa
    66541 gccaaatatt gcttgtactt catatttatc caacaatcct gactgaataa cgctttgtgc
    66601 gccaccggaa atttcttctg atggctgata gatcagtact acattataaa taagatcctc
    66661 aatgtgttta gttataaagt cagtcaaccc taaaagcatc gccatatgtc cgtcatgacc
    66721 acatgcatgc atataacctt catgcttaga agcaaaagag agattcgttt cttctttgac
    66781 tggcagtgca tcgatatctg ttcgaaaagc aatcgtttct tcttgctgat tatcaaaata
    66841 aaggagtagt cctgtttcgc cgatttcatg gataacggcc tcgctgtttt ttacttgttc
    66901 atataaatat ttttttgtct tgaattcatg aaacccgatt tcaggaattt gatggagtgc
    66961 cattcgatat tctttcagca tccgtattca ccatcccttc atagataccc ttcactactg
    67021 tttcatattg attattcggt acaccgatga tcagattcaa ctcctgtgct ccttgtgata
    67081 agatcgatgt gcgaatttct aatttgttca atatcgataa cactttccca gataatccaa
    67141 tcaattcttt atgcggaccc ccaacgacag agatcaaagc taagtcttcg atcacgacga
    67201 tttcttccac gccaagtgtt tcttttaatt ctttcgtgat gagaaataac ttatcggcaa
    67261 tcgcttctgc tgagacgatc actccgatat tgtcgatccc tgaaggaata tgttcgattg
    67321 atatctcatg tttagcaaaa atgctcattg ctttccagat aaacccgatt tcatcggaca
    67381 tatgacgttt gaataaagtg atggaaagaa aatccttgcg tccagctatt cctgtcagtc
    67441 cattttcctt ttctatttcg tgattcgata tttttgtccc ttgagcgaaa ggatcattgg
    67501 tattttttat ttggatgggg atatcagcct cttgaactgg gaaaacagct gcttcatgaa
    67561 gaacgttagc ccccatataa gccatctctc tcagttctct aaaagagatt tcctcgatga
    67621 ttttaggatg atcgatgatt cgcggatcag ccatcatgat tcctgaaaca tccgtccaat
    67681 tctcataact ttccgcttgc aacatcttcg ccagtatcgc gccggtaatg tcgcttcccc
    67741 cacggcccaa taatttttct tttcctgcag ggttcgcccc gtaaaaccct ggaacaacca
    67801 ttttcggata tcttttaaat gcttttgtga tttcctgtgc tgttttttcc tgatcgattt
    67861 tcccatcata gtcaaagaaa atcaggtctt ttgcgtcaat aaactggtaa ccaagatact
    67921 cagccatcag ttgagcagtc agatattcac ctctacttac taaataatct tcagttatct
    67981 tttcgtcttc cagttgtttc tgtatgcgtg ctagttcatc ttcaataggt aactgcaggg
    68041 caagaacatc tcttacttga atgaaacgat cacaaatcct tttccaaagg ggtgtccaat
    68101 caacatgatg ctgaaggtaa gcataaatca aataaagtaa gtctgttact ttgtcatcat
    68161 ctgacgtacg tttgcctaaa gcagaaacaa cgatcacttg gcgattggga tctgaaagaa
    68221 caattgtacg gacttttttg aattgctctt catgagacat cgatgttccg ccgaatttcg
    68281 ctactttcag cgtattactc atcttgccac ctccgctatt ttctgcatta aagctgcagc
    68341 aatctgtacg gcattcgcag cggctccttt gcgtatgtta tcggcagtcg tatagaacaa
    68401 caatccgttc tctaaagaag tgtcttttct gatcctccca acataaacca tatctgttcc
    68461 tctagcaaga atgctcgtcg gatatttatg tgctgctaga tcatcttcta attgaatccc
    68521 tggaaactgg ctgagacact ctctaatttc ttccgtcgta aactcttttt gcagttgaac
    68581 tgccacagag actccatgtc cattttcgat tggcacacgt acgcaggttg ctgatactgg
    68641 caaagcaggc tgatgcagaa tcttacgggt ctcatttatc attttctgtt cttcttttgt
    68701 atacccattt tccaaaggct gatcgatttc cggaatcacc gtttggctaa tattataagg
    68761 atacaaaact ggttcctcgt cattttgcgt acgatgaaga tcttcaatgc ctttttgccc
    68821 ggaaccagaa actgcttgat aggtcgaata attgactcgc tggataccaa aggcttcttg
    68881 taaagcttta agcggaagta ccgcttgaat ggttgaacaa tttggatttg caatcagccg
    68941 attcaatgta tagtcttcca gattgacctc tggaacgatt aatggaatat ccgaatcttc
    69001 ccgccatgct gaggaattat ctatgacaaa tagtccctga cttcgtgcga ttggcgcaaa
    69061 ctctttggat acagaagcac ctgcagaaaa tagcgctaaa tcatatcctt caaatgaagt
    69121 ttctgtcaat tcctgtacag tgtgtttctt tttcttgaat acacaaactt taccggctga
    69181 acgttttgaa gctaataatt tcaatcgtcc tactggaaaa tcatattctt ctaacacttt
    69241 gagcatcgtt tggccgacta atccggttgc ccccacaata caggtatcta tttcgcgcat
    69301 ttttctccat ctcctctata attcgcgtaa cgcagcaatc aatgctgtat tttcttttgt
    69361 ctgatcatca cttattttta atacacgtgc aggtattcca ccgacaacag tttctgctgc
    69421 cacatcttct aaaaccactg ctcctgctgc cacgactgca tcttttccga tatggacacc
    69481 ttcaacaatc actgcattcg cacctaccag tacgccgtct tctacgataa ccggtttggc
    69541 cgatgctggt tcgatcacac ctgctaagac agctcctgca ccaatatggc agtttttgcc
    69601 cactgtcgct cgtccaccta agacagcacc catatcaatc atcgtgtttt caccgattac
    69661 tgcaccaata ttgataatcg ctcccatcat gatcacggca ttgttgccga ttgacacttg
    69721 atctctgata atagcacctg gttcgatacg agcattaatt tttttaatat ctagcaaagg
    69781 aactgcgctg tatcttgcag cagtttcgat ttcgatttca ataatttttt ctgattcagc
    69841 ttctaaaacc ggagcaatcg tctcatagtc accaaaaaca acgaagctgc ttccttcacc
    69901 aaaaacttta ctttctttaa attcgattgg ctcggttaca ttcagataga ctttcacagg
    69961 cgtcttttta gatgccgttt gaatgtagcg gatgatttct tgagcttcca cgttttttac
    70021 acccctttgt ccattaataa atcttctaat ttgtaataac ctggtgactg atctttcaac
    70081 cagtccgctg cttctactgc gcctgcagca aagattcgat tagaaaaaga ttcatgtttg
    70141 atcgtaaata tttcatcagt agcagcaaac aatacatcat gttcaccagg taaagaacct
    70201 gcacgaatac tgtgtacacc aatttggttt tcttctcttt ttttcccttg ccagttgtat
    70261 acgggctgca gcttttgcac ttcttggatt gtttgaagca gcatatttgc agtaccagat
    70321 ggtgcatctt ttttacgact atgatgtttt tcaatcagtt cgatctgcca gccttgcaac
    70381 attgcagctg cttgcttgat tagttgattc atcaccatga tgccgaacga aaaattagca
    70441 ctatacatca caggtacatg tttggcatgt tcttttattt cttggaactg tgattgtgta
    70501 taacctgtcg tgccaatgac taacggaagt tgatatgata agctatacga aaggattttc
    70561 tctaaattat ctggatgaga aaaatcaata agcagttctg gcagttccgt aaaagcaagc
    70621 gtagttgctt ctttaggtgc cgtccgtaaa agaaggaccg tatctcctct ttcttgtgca
    70681 acttcttcga ctctatttcc catctgtcca ctgccaatca gtccgatttt catttttcaa
    70741 ccgtcctttc ttttaacaaa agctgagcta attgtttctt tttctctttt gaaagagaaa
    70801 ctaaaggcag gcgtagaggt ccggaaggta agtctaataa tgacatggct gctttgacag
    70861 gaataggatt ggtctcactg aacaaggcat ggatcagttc aagtgcattt aattgctttt
    70921 cttttgcttg ctgccatctt ccgtcctgcg tatcttttac taattcatgg actattttag
    70981 gttggatatt cgcccataca cttattacac cgcttcctcc tacactcatt actggtagga
    71041 tcaaatcatc atttccactg taaaggaaga attcttctcc aatcaaccga gcggcatcca
    71101 tcacataagc catatctcct gaggcttctt tcaaaccaat gatattagga tgttctgcta
    71161 aatttactaa tacatggatt ggaatcgaca tgcctgttct agaaggaaca ttatacaaaa
    71221 taagcggaat cggtgaggct tctgcaattg ctgtaaaatg agcagctagt cctgcatcac
    71281 ttgttttgtt gtagtaaggg gtgatgacca aaagtgcgtc tgcccccaaa gaagcaaaat
    71341 gcttcgcttt ttcaatcgtt tctttcgtat tattcgttcc agctcctgca atcaccggca
    71401 cacggccagc cactttcttt actgtcagtt gaaggatctg ctcctcctca gattgtgtga
    71461 gcgtagaaga ttctcctgtt gtacctaaaa ctaataatcc atctgtttgt tcatctaaat
    71521 ggaattggat caatttttcc aaaccagcat aatctactga tccctcttca ttcatcggcg
    71581 tgatcagcgc cacgatcgaa ccttttatca tcttcattcc tccaatattc ttgtccattc
    71641 atgaactgct gggccagcca tttgtatcgc atcctcacca gagatcatca agtctccttg
    71701 ttccaagtga acaactacct gatctgctga aattttccca gtttccttag caaccacata
    71761 agaggcacag caacctgtac cgcaagcaag tgtccaacct actcctcttt cataggtacg
    71821 gacgatcaat tcttgttcat tgactacttc tacaaagttg acattggttt tttctttgaa
    71881 caaaggatga tgacagatca gttcacctct ttttctccag acttcctcac gcgcattttc
    71941 aacaaaaaca acggtatgaa tcgttcctaa aaataaacta gtgatcgtta ctttttcacc
    72001 attgatctct atttcctggt tttttattgg ataagtttga ttagctttga ttttttccgg
    72061 agaatcatct ggcactccca tatctacgct gcaataaaaa ggtgagggat ccaacaccgt
    72121 aatttttctt gtgcctgcta atgtctctac gttgaattcc tgttcattca tcatgttctg
    72181 atcatttacg tatcgagcaa aacagcgaat gccatttccg cacatcggcg cgcggctgcc
    72241 atctttgttg taatagatca tttccaacgg atcgattttt acggcgatca atccgtctat
    72301 atcatatttt tcttttgtgc ataaagaagc ggctaattga ctataatctg tgtcttctgc
    72361 gtaatctaat aagataaaat catttccgca gccattatat ttatgaagga tcataggaaa
    72421 cttcccccct tagtagatca gaaaatgctt gtctgcgaac aatcagtctt gattggccat
    72481 tattgacaaa aacaactgct ggtcgtgtcg ctcgattgta gttgctgctc atcgagtatc
    72541 cataagctcc cgtggaataa acagctaaaa gatcacccga ttctgctttc ggcaaataag
    72601 cttctttaat taccacatcg cctgattcac acatttttcc tgcaatcgtc actttctctg
    72661 ttttttcttc cgacagtttt gctgctaaat cacaatcgta ttctgcctga tacagagctg
    72721 gacggatatt atctgtcatc ccgccatcta caaagtaata ttttttatgt ggtgtctttt
    72781 tgatgaaacc aatcgtgtat agtgttgtac cagcttctcc aacaatactt ctacccggtt
    72841 cgatcagtaa ttgacgaatg gtcagttttc gtttttccaa agcttcttcc gtatattcaa
    72901 tcagtttttt tagtgcctct tcgactggga tcggctgatc attttgtgta taacgaatcc
    72961 caaaccctcc gccaagattc aaggaaagag gttggtcaaa atcggttaag taattcgtca
    73021 atttgtcgat ttcagctaac caagcagtaa catcaaaaat ttgtgacccg atatgagcat
    73081 gaaatccttc taactctaga tagctgcttt cttttatcaa ctgaagggct tgttggcact
    73141 ctttactttc atagctcatg ccgaattttg aatcaatatg cgcagtgacg atatattcat
    73201 gagtatgtgc ttctactccg acattcaacc gaagcatgat acagattttt tttaaatagc
    73261 gttggcttaa ttctgctaat aattcaactt ccatcaaatt atcggcaaca atatgcaaca
    73321 aatcattttc aagtgcatag atcagttcct ctggcgtctt gttgttacca tggaagtaga
    73381 tagtatctct aggaaaatca gcttgcaaag cggtatagat ttccccacca ctgacaacat
    73441 ctagcccgaa tccatattca cgaactaaat gcagcatctc aatcgtttgg aatgctttag
    73501 aggcgtaaag cacttttgta tcaaaacgtt tcgatacaaa gcctcgcttg aatgacagca
    73561 tcgtttgtct catcttttct tcatcataca catacagagg tgtcttatac ttttctgcca
    73621 atgtgcttgc tgtgactccg ccaattatca attctgtcat tttttcattc ctcttttcac
    73681 tttttttccg tttttattgg aatttcattt gataaagatt tgaattttat tggaataata
    73741 ttaaacaaaa ttttctgaat tgtctagtat atttcgaaat atttttttaa aaataagaat
    73801 aaaaatggtt ataaaaacaa aatacccgaa ctattagaag ctatttatac ataaaaacat
    73861 aaattcaaca ctatttagtg atgaaaaaat attttcgctt caatatagtt cggaatagtt
    73921 cggatatttt caattttatg aatcatttca atttcttcat aaataagtgc agttttttct
    73981 ttctctagct tttgccattc atctttcaaa atataaataa aatcaaatac ttttttattt
    74041 ttcaatttgc tgattccaca aaaaacctct tgaaaggcag atccgatgat ctgcctttca
    74101 agaggtcaag ctctgttttc aaattcttat caatccgtta gcgaattatt ttgacgcacg
    74161 ttttacataa gaaccttctg ctgtattgat ttccaacaat tcatctgttt cgatgaagtc
    74221 aggaacattg actactaagc cagtttccat tgtcgctggc ttacctgaac cagtgactgt
    74281 tgcacctttg atggaaggtt gtgtttccac aacacgtaat acgacagttg ttggtaattg
    74341 aacgccgatg acttcgctac cgaaaaattg gatttttact tccatgtttt ctaggatata
    74401 tttcatttct tcttctactg tttctacagg aatttcatat tgctcatatg tttccaaatc
    74461 catgaagaaa gctgtatcat ccatcgtgta caaatattgt acaggttttg tgtcgatatg
    74521 tgctttttca aatttttctt ctggtctgaa cgttgtgtcg tatgttgcac ctgaacggac
    74581 atctttcagt ttcatacgca taactgtatt tcctttacca ggtttatggt ggcttgcatc
    74641 cagaacttta atcagtttgc cgtcttgtac gaaagtcata ccggctctta aatcacttgc
    74701 tgagatcatt atttttgctc cttcttttct ttatcataat caactttatt gtatcagaca
    74761 accggccaga aaactagagt attctctatc ttttaaggtc tcatgcctat ttgtcagaaa
    74821 aataaacacg ggtaaacgat gctatcgaaa tatttttttc tttcaagcct ttgactttta
    74881 ttttttttca tgctatcgtt aataaaaatt ttacttggag ggatacacat gacagtcagc
    74941 aaccaaaaac aattgaatcg ttgctgttgt atggtgcata aaagcagcat actatttgtc
    75001 atgtctatta attgcccttc atagaatgag taccaggtaa cttatctttt tatctactca
    75061 atttttctct atgtctttca aggagctgat ttaatagtca gctccttttt ttattatctt
    75121 tttttaggag gcccacatga ttatctcgac aattacagaa gctattggtt ccacaccgct
    75181 ttatcgcttt gacaatcagc actattctat gcctaaaggt tctgctattt atgcaaaact
    75241 agaatattta aatcctggag gaagtatcaa agatagattg ggcagttatt tgatcaaaga
    75301 aggatttgcc caaggaaaaa tcaatgaaaa aacgacgatc atcgaaccaa ctgccggaaa
    75361 tacaggaatt gggatcgctc tggccgcact cacatacaaa ttaagaacgg tttttgttgt
    75421 tccagagaag tttagcttag aaaaacaaca attgatgaaa gcattgggag caaagatcat
    75481 ccattctcct agtgagcaag gaatcatcgg tgccatcagc aaaagtaaaa aacttgcaga
    75541 ggagatatca aacagctatc tgcctcttca atttgaaaat aaagataatc ctgctgctta
    75601 ctatcatacg ttagggccag aaatctttca tgaaatcaaa gaaaatatcc atagttttgt
    75661 agcaggaatt gggagtggtg gaacctttgc tggaacttct acctttttga aagaaaaata
    75721 tcccgacatt cgaataatcg gtgtagaacc agagggctct gtgttaaatg gtggtgatcc
    75781 agcccctcac gaaattgagg gtatcggtgt agaattcatt ccgccgtttc tttctcccct
    75841 ttcaatcaat cagatcgaga caatctctga tgtagaaggg tttaactata ctaggcagtt
    75901 agcacgagaa caaggactat tagttggaag ttcgagtggt gcggcttttg cagcagcatt
    75961 aagagaaatt cgtcgtttac cacctggtca tcgtgtcgtt acgatttttc cagacgcagc
    76021 agaccgctat ttatcaaaaa acatttactt ataggaggaa agaaacatgc acattcaaac
    76081 aaaattgatc cacggaggca tcagtgaaga tcccacaaca ggagctgtca gtgtacctat
    76141 ttatcaaaca tccacttatc ggcaagatgg cgtcggccag cctaaacaat atgaatattc
    76201 aagatctggt aatcctactc gttttgcatt ggaagaactg attgctgatt tggaaggcgg
    76261 tgttcgagga ttcgcttttt cttctggtct ttctgggatt catgccgttt tttctctttt
    76321 tcaagctggc gatcatatct tattaggaga tgatgtctat ggaggaacat tccgtctatt
    76381 tgacaaagtt cttactaaaa atggtctcga gtacacgatc atagatacga gtaatcttga
    76441 taaaatagaa caatccatca aacccaatac aaaagcactt tatcttgaaa cacctagcaa
    76501 tccgcttcta aagattactg atttagaaaa aagtgccact ctggctcatc aacatggatt
    76561 gatagttatt gctgacaata catttgctac tccttatttt caaagacctt tagatctcgg
    76621 ttctgatatc gttgtccata gcggtaccaa atatctaggc ggacacagcg atgtagtagc
    76681 tggtttagtg acttctaacc ataaagatct agccgatcag atcggatttt accaaaatgc
    76741 tatcggagcc gtacttggtc cccaagatag ctggttactt cagcgcggaa tcaagacact
    76801 aagtgttcgt atggaggaac atcaaaaaaa tgcttttgtg gtagctgatt ttctattttc
    76861 tcatccagca gtagaaaagg tctactatcc aggattaccc gatcatgaac tccatggagt
    76921 tgctaagcag cagatgagcg gctttagcgg aatgatttct tttactttaa aaaatgaaga
    76981 atctgctatt ccatttgttg aatcactcca actatttact ttagcagaaa gtttaggtgg
    77041 cgtagaaagt ttagtggaaa ttccttctgt catgactcat gcgtctattc caaaagaaaa
    77101 acgagaagag gctggcataa aggacggctt gatccgtctt tctgtaggaa ttgagtatgg
    77161 tcaggatctc ataaacgatt tagctcaggc atttgacaga atcaagaact agaagtccca
    77221 ataaacgact tagcagaaaa atctaaaaaa tatttcagat tgctacacct tggaaacgat
    77281 aagtgttaga atcaagtaga aagacgtttt tgtaaatttc actaaataat tctttagtga
    77341 aaagcatcaa aacgaatgaa caaacagata ggagtcttac aaatgagaat gaatgtcgaa
    77401 agttttaatc ttgaccatac aaaagtcaaa gcaccttatg tacgtgtagc tgatcgaaaa
    77461 acgggtgcgc atggcgatgt catcattaaa tacgacgtcc gctttaaaca accaaataaa
    77521 gaacacatgg acatgccaag tctacattca cttgagcact tgactgccga actaatccgc
    77581 aatcacgcag attacatcgt agactggtct ccaatgggat gccaaacagg cttttacttg
    77641 actgtattga atcatgaaag ctacgaagac attttagatg ttcttgaagc aacaatgaaa
    77701 gatgttcttg aagctactga agtacctgca agcaatgaaa cacaatgtgg ctgggctgcc
    77761 agccatacac tagaaggtgc acaagctttg gctcgtgaat ttttagcaaa acgcgatgaa
    77821 tggtctgaag tagaagcata gtttctaaaa gaacgtaaag atgagaagca atatagctat
    77881 cttatcttcg tattttatta aatatttatt gtctgaaaag agttctaaac gggaaaacaa
    77941 atagctagtt ttcagtttag cgctcttttt attatataaa atagaaagga gaaaacttta
    78001 tgtctattta taattttgaa actactttag aagacggcac tgtctattca ttgaaaaaat
    78061 atgcaggcaa accgataatc attgttaaca ctgcaacaaa atgcggtcta gcacctcaat
    78121 ttaaacaatt agaagaactt taccaagctt atcagacaca aggtctggtt atcttaggct
    78181 ttccttccaa tcaatttaaa caagagctag attcagcgac tgaagcagca gaagcttgta
    78241 aaaccactta tggcgttacc ttccctatgc atcaattgat tgacgtaaat ggagaaaata
    78301 cagatcctct ttttacttat cttaaaaatg aagctcctgg tactgtcact aaagcaataa
    78361 aatggaattt tactaaattt ttgattgata gaaatggcaa agtaatcgaa cgatttgcac
    78421 cacaaacttc tccattgaaa atgaccgctg caattgaatc cgttctttaa cttaataaaa
    78481 ataaaaaaag atgaactatt tctagttcat ctttttgcct ttgcgtggcg acgtcctact
    78541 ctcacaaggg gcaacccctc actacaatcg gcgctaagaa gcttaacttc tgtgttcggc
    78601 atggttacag gtgtatcctt ctcgctatcg ccaccacact gtggtgttat cttttattga
    78661 gtaaattttg ttcactcaaa actggatttg aagtca
//
LOCUS       DACIFS010000005        66817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.005, whole genome shotgun sequence.
ACCESSION   DACIFS010000005 DACIFS010000000
VERSION     DACIFS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.005"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="IYA83_000328"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="IYA83_000328"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ8585794.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="IYA83_000329"
     CDS             1013..2260
                     /locus_tag="IYA83_000329"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ8585795.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="IYA83_000330"
     CDS             2336..2680
                     /locus_tag="IYA83_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585796.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="IYA83_000331"
     CDS             complement(2797..3123)
                     /locus_tag="IYA83_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585797.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="IYA83_000332"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="IYA83_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="IYA83_000333"
     CDS             4814..5329
                     /locus_tag="IYA83_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ8585798.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="IYA83_000334"
     CDS             complement(5528..5974)
                     /locus_tag="IYA83_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ8585799.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr"
                     /locus_tag="IYA83_000335"
     CDS             complement(6021..7499)
                     /gene="msr"
                     /locus_tag="IYA83_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ8585800.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="IYA83_000336"
     CDS             complement(7853..8482)
                     /locus_tag="IYA83_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ8585801.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="IYA83_000337"
     CDS             complement(8466..8930)
                     /locus_tag="IYA83_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8585802.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="IYA83_000338"
     CDS             complement(9417..10685)
                     /locus_tag="IYA83_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ8585803.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="IYA83_000339"
     CDS             complement(10916..11653)
                     /locus_tag="IYA83_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8585804.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="IYA83_000340"
     CDS             complement(11720..12289)
                     /locus_tag="IYA83_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ8585805.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="IYA83_000341"
     CDS             12417..13289
                     /locus_tag="IYA83_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8585806.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13631..14503
                     /locus_tag="IYA83_000342"
     CDS             13631..14503
                     /locus_tag="IYA83_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8585807.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14500..16458
                     /locus_tag="IYA83_000343"
     CDS             14500..16458
                     /locus_tag="IYA83_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585808.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16709..17560)
                     /locus_tag="IYA83_000344"
     CDS             complement(16709..17560)
                     /locus_tag="IYA83_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8585809.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17683..18150
                     /locus_tag="IYA83_000345"
     CDS             17683..18150
                     /locus_tag="IYA83_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ8585810.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18413..18895)
                     /locus_tag="IYA83_000346"
     CDS             complement(18413..18895)
                     /locus_tag="IYA83_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ8585811.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18995..19858)
                     /locus_tag="IYA83_000347"
     CDS             complement(18995..19858)
                     /locus_tag="IYA83_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ8585812.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20456..21304
                     /locus_tag="IYA83_000348"
     CDS             20456..21304
                     /locus_tag="IYA83_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585813.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21475..21816)
                     /locus_tag="IYA83_000349"
     CDS             complement(21475..21816)
                     /locus_tag="IYA83_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ8585814.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21902..22609
                     /locus_tag="IYA83_000350"
     CDS             21902..22609
                     /locus_tag="IYA83_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ8585815.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22596..22919
                     /locus_tag="IYA83_000351"
     CDS             22596..22919
                     /locus_tag="IYA83_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ8585816.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23008..24045)
                     /locus_tag="IYA83_000352"
     CDS             complement(23008..24045)
                     /locus_tag="IYA83_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ8585817.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24492..25475)
                     /locus_tag="IYA83_000353"
     CDS             complement(24492..25475)
                     /locus_tag="IYA83_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ8585818.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25891..26661)
                     /locus_tag="IYA83_000354"
     CDS             complement(25891..26661)
                     /locus_tag="IYA83_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585819.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26654..27589)
                     /locus_tag="IYA83_000355"
     CDS             complement(26654..27589)
                     /locus_tag="IYA83_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585820.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28183..29832)
                     /locus_tag="IYA83_000356"
     CDS             complement(28183..29832)
                     /locus_tag="IYA83_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ8585821.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30027..30923)
                     /locus_tag="IYA83_000357"
     CDS             complement(30027..30923)
                     /locus_tag="IYA83_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8585822.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30916..31596)
                     /locus_tag="IYA83_000358"
     CDS             complement(30916..31596)
                     /locus_tag="IYA83_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8585823.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31597..32379)
                     /locus_tag="IYA83_000359"
     CDS             complement(31597..32379)
                     /locus_tag="IYA83_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8585824.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32372..33289)
                     /locus_tag="IYA83_000360"
     CDS             complement(32372..33289)
                     /locus_tag="IYA83_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8585825.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33292..33576)
                     /locus_tag="IYA83_000361"
     CDS             complement(33292..33576)
                     /locus_tag="IYA83_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8585826.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34036..34428)
                     /gene="rpsI"
                     /locus_tag="IYA83_000362"
     CDS             complement(34036..34428)
                     /gene="rpsI"
                     /locus_tag="IYA83_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ8585827.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(34444..34887)
                     /gene="rplM"
                     /locus_tag="IYA83_000363"
     CDS             complement(34444..34887)
                     /gene="rplM"
                     /locus_tag="IYA83_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ8585828.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(35406..35873)
                     /locus_tag="IYA83_000364"
     CDS             complement(35406..35873)
                     /locus_tag="IYA83_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ8585829.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(35993..37531)
                     /locus_tag="IYA83_000365"
     CDS             complement(35993..37531)
                     /locus_tag="IYA83_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ8585830.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            37611..38297
                     /locus_tag="IYA83_000366"
     CDS             37611..38297
                     /locus_tag="IYA83_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ8585831.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(38498..39025)
                     /gene="msrA"
                     /locus_tag="IYA83_000367"
     CDS             complement(38498..39025)
                     /gene="msrA"
                     /locus_tag="IYA83_000367"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ8585832.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(39109..39786)
                     /gene="pgmB"
                     /locus_tag="IYA83_000368"
     CDS             complement(39109..39786)
                     /gene="pgmB"
                     /locus_tag="IYA83_000368"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ8585833.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(39783..40913)
                     /locus_tag="IYA83_000369"
     CDS             complement(39783..40913)
                     /locus_tag="IYA83_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ8585834.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(40913..43096)
                     /locus_tag="IYA83_000370"
     CDS             complement(40913..43096)
                     /locus_tag="IYA83_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ8585835.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(43093..44118)
                     /locus_tag="IYA83_000371"
     CDS             complement(43093..44118)
                     /locus_tag="IYA83_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8585836.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(44163..44990)
                     /locus_tag="IYA83_000372"
     CDS             complement(44163..44990)
                     /locus_tag="IYA83_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ8585837.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(44987..46054)
                     /locus_tag="IYA83_000373"
     CDS             complement(44987..46054)
                     /locus_tag="IYA83_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ8585838.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(46080..46916)
                     /locus_tag="IYA83_000374"
     CDS             complement(46080..46916)
                     /locus_tag="IYA83_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8585839.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(46913..47782)
                     /locus_tag="IYA83_000375"
     CDS             complement(46913..47782)
                     /locus_tag="IYA83_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8585840.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(47852..49135)
                     /locus_tag="IYA83_000376"
     CDS             complement(47852..49135)
                     /locus_tag="IYA83_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ8585841.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(49149..50834)
                     /locus_tag="IYA83_000377"
     CDS             complement(49149..50834)
                     /locus_tag="IYA83_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ8585842.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            51109..52134
                     /locus_tag="IYA83_000378"
     CDS             51109..52134
                     /locus_tag="IYA83_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8585843.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(52729..56382)
                     /gene="rpoC"
                     /locus_tag="IYA83_000379"
     CDS             complement(52729..56382)
                     /gene="rpoC"
                     /locus_tag="IYA83_000379"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ8585844.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(56461..60078)
                     /gene="rpoB"
                     /locus_tag="IYA83_000380"
     CDS             complement(56461..60078)
                     /gene="rpoB"
                     /locus_tag="IYA83_000380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ8585845.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFVDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            60806..61774
                     /locus_tag="IYA83_000381"
     CDS             60806..61774
                     /locus_tag="IYA83_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8585846.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            61792..62697
                     /locus_tag="IYA83_000382"
     CDS             61792..62697
                     /locus_tag="IYA83_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585847.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            62694..63500
                     /locus_tag="IYA83_000383"
     CDS             62694..63500
                     /locus_tag="IYA83_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8585848.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            63657..64625
                     /locus_tag="IYA83_000384"
     CDS             63657..64625
                     /locus_tag="IYA83_000384"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ8585849.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(64942..65466)
                     /locus_tag="IYA83_000385"
     CDS             complement(64942..65466)
                     /locus_tag="IYA83_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ8585850.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            65753..>66817
                     /gene="scm"
                     /locus_tag="IYA83_000386"
     CDS             65753..>66817
                     /gene="scm"
                     /locus_tag="IYA83_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ8585851.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        19352 a        14204 c        12266 g        20995 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt tcttaattta
    13381 tttcttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    13441 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    13501 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    13561 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    13621 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    13681 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    13741 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    13801 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    13861 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    13921 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    13981 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    14041 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    14101 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    14161 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    14221 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    14281 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    14341 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    14401 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    14461 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    14521 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    14581 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    14641 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    14701 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    14761 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    14821 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    14881 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    14941 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    15001 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    15061 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    15121 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    15181 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    15241 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    15301 aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt ctttactggt
    15361 gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata tgtcttgcat
    15421 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    15481 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    15541 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    15601 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    15661 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    15721 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    15781 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    15841 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    15901 gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc acaaacaaac
    15961 accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat ttacgctatc
    16021 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    16081 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    16141 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    16201 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    16261 tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    16321 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    16381 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    16441 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    16501 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    16561 aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt ttatttgact
    16621 cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata ctcaaaacta
    16681 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    16741 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    16801 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    16861 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    16921 ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    16981 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    17041 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    17101 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    17161 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    17221 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    17281 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    17341 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    17401 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    17461 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    17521 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    17581 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    17641 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    17701 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    17761 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    17821 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    17881 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    17941 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    18001 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    18061 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    18121 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    18181 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    18241 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    18301 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    18361 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    18421 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    18481 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    18541 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa
    18601 caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg
    18661 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    18721 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    18781 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    18841 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    18901 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    18961 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    19021 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    19081 cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    19141 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    19201 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    19261 gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta
    19321 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    19381 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    19441 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    19501 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga
    19561 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    19621 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag
    19681 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    19741 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    19801 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    19861 ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    19921 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    19981 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    20041 ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc
    20101 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    20161 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    20221 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    20281 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    20341 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    20401 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    20461 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    20521 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    20581 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    20641 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    20701 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    20761 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat
    20821 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    20881 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    20941 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc
    21001 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    21061 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    21121 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    21181 agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga
    21241 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg
    21301 gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc
    21361 acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga
    21421 agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa
    21481 gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat agattttatg
    21541 atacttttct acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg
    21601 acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc
    21661 taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa tatcagcttg
    21721 gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct
    21781 gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta
    21841 gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt
    21901 attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg
    21961 ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact
    22021 agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag
    22081 catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc
    22141 ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca
    22201 aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt
    22261 gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta
    22321 tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc
    22381 gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca
    22441 agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt
    22501 gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac
    22561 actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc
    22621 tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac
    22681 tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca
    22741 ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga
    22801 tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa
    22861 gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa
    22921 taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa
    22981 ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt
    23041 tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat
    23101 cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc
    23161 aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa
    23221 catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc
    23281 tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc
    23341 aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc
    23401 acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt
    23461 cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc
    23521 tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc
    23581 gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt
    23641 caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc
    23701 gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc
    23761 tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac
    23821 tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg
    23881 aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga
    23941 actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa
    24001 cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta
    24061 gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg
    24121 ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    24181 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc
    24241 ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct gttttttgaa
    24301 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat aaacggtgtt
    24361 caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag ctatttttgt
    24421 ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat tcacggtgtt
    24481 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtctctaag
    24541 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    24601 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    24661 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    24721 ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa aatccctcgg
    24781 ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt ctggcgatcg
    24841 gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg taaagcaatg
    24901 attttaggaa tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    24961 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    25021 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagct
    25081 gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc ttttccacgt
    25141 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    25201 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    25261 atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt tccatctgga
    25321 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    25381 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    25441 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    25501 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    25561 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact gtgacaaaaa
    25621 tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt tgcggtaata
    25681 agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat
    25741 gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    25801 ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca
    25861 ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat
    25921 caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga tcatcgatgt
    25981 tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac
    26041 gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga tggcatagaa
    26101 aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcatc
    26161 ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga ttgccgtcat
    26221 caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga
    26281 aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa tcacaaagcc
    26341 ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat ttttctgaaa
    26401 tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag cagacatcat
    26461 aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac cgatccgccc
    26521 accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg gcggaaccag
    26581 tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc
    26641 agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca
    26701 agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc
    26761 ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg
    26821 tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta tcatatggcg
    26881 ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc atacttgtat
    26941 tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt tcagcttctt
    27001 ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct cgtagaaact
    27061 cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc aagatcagca
    27121 aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa
    27181 gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc
    27241 tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt
    27301 gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca atctgttgct
    27361 ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt ccagatgttt
    27421 tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca tttggcccta
    27481 atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat gcttcgaccc
    27541 cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt tatttcctat
    27601 ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa aaacaacttg
    27661 gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag ttcatgatca
    27721 tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa aagtaccgac
    27781 agcaactact acgatgacat atagaagatc cttataactg aggttcttgt cttttttatt
    27841 catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt taaccgaaat
    27901 aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt acggagaact
    27961 ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa gctgctagaa
    28021 ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga tcatctggac
    28081 agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact tcttttgata
    28141 atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa acggatacca
    28201 ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta tggatttagg
    28261 ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
    28321 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
    28381 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
    28441 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
    28501 tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
    28561 cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
    28621 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
    28681 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
    28741 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ctggattaac
    28801 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
    28861 ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg ctgcaacttc
    28921 caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
    28981 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
    29041 caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag ggatgtctct
    29101 taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt ttgctaactg
    29161 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
    29221 ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg ctagatagat
    29281 tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca agccaagaaa
    29341 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
    29401 aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt ctctcagttg
    29461 aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc caaaatcaaa
    29521 gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac tgttgatccc
    29581 ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt cactatccca
    29641 tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt tatcaaatct
    29701 cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt tcaaacgtga
    29761 aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa cgataaattc
    29821 tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag cactgttgcg
    29881 ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta tcaatcacac
    29941 caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca attttttgtc
    30001 attcacttca ggtaaattag agccaattaa accctccgca acttgacgga gatggagaag
    30061 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
    30121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
    30181 cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt ggccactgtt
    30241 aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
    30301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
    30361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
    30421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
    30481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
    30541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
    30601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
    30661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
    30721 tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt cttttctcga
    30781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
    30841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
    30901 agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
    30961 agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca ctgttttctc
    31021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
    31081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
    31141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
    31201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
    31261 taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
    31321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
    31381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
    31441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
    31501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa cagcttcatc
    31561 gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag atttttggaa
    31621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
    31681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
    31741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaacgaaggc
    31801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
    31861 tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata caggaaatac
    31921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
    31981 tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg tgatcaaaag
    32041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
    32101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
    32161 cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac cgttgatgac
    32221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
    32281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
    32341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
    32401 gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca ggaacttttg
    32461 cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
    32521 cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
    32581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
    32641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg gcgatctgtg
    32701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
    32761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
    32821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
    32881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
    32941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
    33001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
    33061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
    33121 aacccataat agttctcata aaggtagact tacccgctcc attctcccca atcaaaccat
    33181 agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca gcaccgtatt
    33241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
    33301 tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
    33361 aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc gcagacgtgg
    33421 ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    33481 catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg gaacttctcg
    33541 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    33601 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    33661 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    33721 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    33781 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    33841 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    33901 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg
    33961 attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc
    34021 gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc
    34081 tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc
    34141 taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc
    34201 tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt
    34261 ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc
    34321 ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc
    34381 aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc
    34441 ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc
    34501 tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag
    34561 catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc
    34621 agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc
    34681 ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc
    34741 caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga
    34801 aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc
    34861 tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg
    34921 acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac
    34981 ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt
    35041 acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga
    35101 ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa
    35161 ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt
    35221 cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc
    35281 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt
    35341 ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac
    35401 agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt
    35461 ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct
    35521 ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt
    35581 gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg
    35641 ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg
    35701 gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat
    35761 gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg
    35821 cttagatctg caactaattg atttaatact tctttggttt gttggaattt catctttctt
    35881 gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa
    35941 aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt
    36001 cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct
    36061 tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta
    36121 ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc
    36181 cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga
    36241 aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa
    36301 acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc
    36361 gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt
    36421 gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac
    36481 caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg
    36541 aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta
    36601 aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta
    36661 cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct
    36721 gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac
    36781 caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata
    36841 tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg
    36901 tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt
    36961 ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc
    37021 tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag
    37081 tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca
    37141 ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca
    37201 gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg
    37261 ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa
    37321 tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa
    37381 agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa
    37441 aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg
    37501 ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca
    37561 aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt
    37621 attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt
    37681 taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt
    37741 ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca
    37801 ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct
    37861 ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt
    37921 tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt
    37981 cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc
    38041 tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg
    38101 gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga
    38161 tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata
    38221 aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc
    38281 ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata
    38341 gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac
    38401 taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc
    38461 tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc
    38521 gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt
    38581 tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt
    38641 ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg
    38701 caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat
    38761 cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact
    38821 gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg
    38881 tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg
    38941 tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc
    39001 ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt
    39061 tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga
    39121 tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat
    39181 cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc
    39241 agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg
    39301 tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac
    39361 ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag
    39421 gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc
    39481 aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag
    39541 atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc
    39601 catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg
    39661 ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc
    39721 gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat
    39781 tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct
    39841 gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga
    39901 aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca
    39961 cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc
    40021 gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct
    40081 tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt
    40141 ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat
    40201 gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact
    40261 gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt
    40321 ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata
    40381 ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact
    40441 ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca
    40501 tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat
    40561 tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt
    40621 gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa
    40681 gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc
    40741 aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc
    40801 agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc
    40861 tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc
    40921 ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag
    40981 gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca
    41041 acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg
    41101 gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta
    41161 gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc
    41221 ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac
    41281 ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt
    41341 catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata
    41401 acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat
    41461 aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg
    41521 gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat
    41581 ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt
    41641 taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat
    41701 attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta
    41761 cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta
    41821 aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac
    41881 tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca
    41941 atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg
    42001 caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt
    42061 acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa
    42121 tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta
    42181 cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca
    42241 tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat
    42301 ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt
    42361 gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct
    42421 tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc
    42481 gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt
    42541 tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt
    42601 tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat
    42661 ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat
    42721 tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa
    42781 tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa
    42841 ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat
    42901 ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg
    42961 caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa
    43021 atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt
    43081 tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc
    43141 caccagattc tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc
    43201 gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt
    43261 cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt
    43321 agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc
    43381 aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc
    43441 atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt
    43501 accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata
    43561 catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc
    43621 tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac
    43681 gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa
    43741 acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt
    43801 tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc
    43861 gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc
    43921 gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg
    43981 tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac
    44041 atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat
    44101 gattgctact ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt
    44161 ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat
    44221 tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc
    44281 atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg
    44341 ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt
    44401 tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg
    44461 atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa
    44521 ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct
    44581 tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg
    44641 cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata
    44701 acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca
    44761 ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct
    44821 gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt
    44881 aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt
    44941 tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc
    45001 ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt
    45061 aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt
    45121 aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt
    45181 aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg
    45241 cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga
    45301 tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt
    45361 caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg
    45421 ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg
    45481 ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa
    45541 tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt
    45601 cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat
    45661 ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac
    45721 gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg
    45781 tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc
    45841 gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga
    45901 taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa
    45961 ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg
    46021 ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt
    46081 tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac
    46141 ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag
    46201 tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac
    46261 aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca
    46321 gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta
    46381 agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt
    46441 agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg
    46501 gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg
    46561 gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt
    46621 tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag
    46681 gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata
    46741 tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct
    46801 tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct
    46861 aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca
    46921 agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg
    46981 atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc
    47041 ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga
    47101 tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct
    47161 tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga
    47221 ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa
    47281 acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga
    47341 aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat
    47401 atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac
    47461 gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag
    47521 ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa
    47581 attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt
    47641 taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca
    47701 ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag
    47761 aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat agctatctac
    47821 ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta
    47881 aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac
    47941 ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt
    48001 gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt
    48061 cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag
    48121 gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt
    48181 cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc
    48241 catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg
    48301 cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca
    48361 tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt
    48421 tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat
    48481 catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg
    48541 ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt
    48601 gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt
    48661 tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt
    48721 ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt
    48781 cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc atcactttag
    48841 caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga
    48901 tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt
    48961 ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag
    49021 agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag
    49081 cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt
    49141 tccacctctt attttattat tttcagccac atatattcat acggctggaa tatcaacttt
    49201 cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt
    49261 aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc
    49321 gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc
    49381 ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact
    49441 gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc
    49501 cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc
    49561 agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca
    49621 gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga
    49681 tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact
    49741 cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata
    49801 cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt
    49861 tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa
    49921 cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc
    49981 tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat
    50041 accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt
    50101 tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag
    50161 cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa
    50221 acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt
    50281 ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc
    50341 acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc
    50401 aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg
    50461 aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg
    50521 ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc
    50581 aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta
    50641 cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct
    50701 tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt
    50761 tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa
    50821 agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat
    50881 attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa
    50941 tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc
    51001 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
    51061 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
    51121 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
    51181 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
    51241 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
    51301 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
    51361 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
    51421 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
    51481 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
    51541 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
    51601 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
    51661 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
    51721 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
    51781 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
    51841 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
    51901 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
    51961 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
    52021 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
    52081 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
    52141 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
    52201 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
    52261 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
    52321 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
    52381 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
    52441 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
    52501 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
    52561 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
    52621 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
    52681 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
    52741 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
    52801 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
    52861 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
    52921 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
    52981 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
    53041 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
    53101 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
    53161 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
    53221 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
    53281 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
    53341 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
    53401 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
    53461 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
    53521 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
    53581 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
    53641 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
    53701 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
    53761 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
    53821 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
    53881 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
    53941 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
    54001 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
    54061 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
    54121 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
    54181 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
    54241 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
    54301 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
    54361 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
    54421 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
    54481 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
    54541 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
    54601 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
    54661 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
    54721 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
    54781 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
    54841 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
    54901 atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc
    54961 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
    55021 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
    55081 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
    55141 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
    55201 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
    55261 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
    55321 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
    55381 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
    55441 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
    55501 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
    55561 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
    55621 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
    55681 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
    55741 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
    55801 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
    55861 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
    55921 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
    55981 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
    56041 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
    56101 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
    56161 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
    56221 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
    56281 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
    56341 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
    56401 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
    56461 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
    56521 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
    56581 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
    56641 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
    56701 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
    56761 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
    56821 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
    56881 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
    56941 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
    57001 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
    57061 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
    57121 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
    57181 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
    57241 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
    57301 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
    57361 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
    57421 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
    57481 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
    57541 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
    57601 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
    57661 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
    57721 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
    57781 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
    57841 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
    57901 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
    57961 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
    58021 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
    58081 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
    58141 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    58201 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
    58261 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
    58321 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
    58381 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
    58441 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
    58501 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
    58561 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
    58621 gtatgtcaat tcgccaagag ggtttgtttg atccacgaac tgtgacaact gagaagaacc
    58681 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
    58741 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
    58801 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
    58861 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
    58921 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
    58981 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
    59041 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
    59101 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
    59161 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
    59221 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
    59281 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
    59341 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
    59401 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
    59461 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
    59521 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
    59581 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
    59641 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
    59701 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
    59761 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
    59821 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
    59881 aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc
    59941 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
    60001 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
    60061 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
    60121 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
    60181 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
    60241 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
    60301 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
    60361 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
    60421 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
    60481 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
    60541 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
    60601 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
    60661 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
    60721 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
    60781 ttccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
    60841 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
    60901 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
    60961 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    61021 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    61081 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    61141 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    61201 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    61261 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    61321 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    61381 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    61441 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    61501 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    61561 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    61621 caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    61681 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    61741 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    61801 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    61861 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    61921 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    61981 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    62041 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    62101 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    62161 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    62221 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    62281 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    62341 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    62401 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    62461 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    62521 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    62581 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    62641 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    62701 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    62761 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    62821 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    62881 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    62941 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    63001 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    63061 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    63121 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    63181 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    63241 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    63301 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    63361 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    63421 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    63481 aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct
    63541 tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa
    63601 actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga
    63661 caacgaaaga caaggtactt caattattga aaaagacacc agattttctt tccggagaaa
    63721 agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg
    63781 aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg
    63841 tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac
    63901 aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca gctcaaggag
    63961 attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac
    64021 ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca
    64081 aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca
    64141 ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg
    64201 gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca
    64261 gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg
    64321 atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc
    64381 agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt
    64441 tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag
    64501 gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa
    64561 caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc
    64621 aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat
    64681 tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag
    64741 gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa
    64801 caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct
    64861 tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta
    64921 agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac
    64981 ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac
    65041 tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa
    65101 gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt
    65161 agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat
    65221 cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa
    65281 atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt
    65341 gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc
    65401 aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata
    65461 atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc
    65521 tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag
    65581 tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat
    65641 gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact
    65701 aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa
    65761 aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc
    65821 cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg
    65881 taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac
    65941 tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt
    66001 tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga
    66061 ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt
    66121 tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct
    66181 tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat atattgattt
    66241 cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc
    66301 taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg atttaactga
    66361 tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac
    66421 gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc
    66481 ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga
    66541 agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat
    66601 cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa
    66661 ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga
    66721 tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac
    66781 agatacatct accacaagtg atagctcaga ttcatca
//
LOCUS       DACIFS010000006        65897 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.006, whole genome shotgun sequence.
ACCESSION   DACIFS010000006 DACIFS010000000
VERSION     DACIFS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.006"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(42..1466)
                     /locus_tag="IYA83_000387"
     CDS             complement(42..1466)
                     /locus_tag="IYA83_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8585852.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            1677..2123
                     /gene="nrdI"
                     /locus_tag="IYA83_000388"
     CDS             1677..2123
                     /gene="nrdI"
                     /locus_tag="IYA83_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ8585853.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(2193..4175)
                     /locus_tag="IYA83_000389"
     CDS             complement(2193..4175)
                     /locus_tag="IYA83_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585854.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4189..5085)
                     /gene="murQ"
                     /locus_tag="IYA83_000390"
     CDS             complement(4189..5085)
                     /gene="murQ"
                     /locus_tag="IYA83_000390"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ8585855.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(5088..6149)
                     /locus_tag="IYA83_000391"
     CDS             complement(5088..6149)
                     /locus_tag="IYA83_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ8585856.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(6361..6888)
                     /locus_tag="IYA83_000392"
     CDS             complement(6361..6888)
                     /locus_tag="IYA83_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ8585857.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6955..8457)
                     /gene="lsa"
                     /locus_tag="IYA83_000393"
     CDS             complement(6955..8457)
                     /gene="lsa"
                     /locus_tag="IYA83_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ8585858.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(8715..9836)
                     /locus_tag="IYA83_000394"
     CDS             complement(8715..9836)
                     /locus_tag="IYA83_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ8585859.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9962..10903
                     /locus_tag="IYA83_000395"
     CDS             9962..10903
                     /locus_tag="IYA83_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ8585860.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(11046..11942)
                     /locus_tag="IYA83_000396"
     CDS             complement(11046..11942)
                     /locus_tag="IYA83_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ8585861.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            12224..13564
                     /locus_tag="IYA83_000397"
     CDS             12224..13564
                     /locus_tag="IYA83_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8585862.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            13759..14202
                     /locus_tag="IYA83_000398"
     CDS             13759..14202
                     /locus_tag="IYA83_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ8585863.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKIAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(14464..15882)
                     /gene="nhaC"
                     /locus_tag="IYA83_000399"
     CDS             complement(14464..15882)
                     /gene="nhaC"
                     /locus_tag="IYA83_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ8585864.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(16286..17509)
                     /locus_tag="IYA83_000400"
     CDS             complement(16286..17509)
                     /locus_tag="IYA83_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ8585865.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(17541..18380)
                     /locus_tag="IYA83_000401"
     CDS             complement(17541..18380)
                     /locus_tag="IYA83_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ8585866.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(18397..19077)
                     /locus_tag="IYA83_000402"
     CDS             complement(18397..19077)
                     /locus_tag="IYA83_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585867.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(19079..20152)
                     /locus_tag="IYA83_000403"
     CDS             complement(19079..20152)
                     /locus_tag="IYA83_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8585868.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(20802..21473)
                     /locus_tag="IYA83_000404"
     CDS             complement(20802..21473)
                     /locus_tag="IYA83_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ8585869.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(21561..24239)
                     /gene="mgtA"
                     /locus_tag="IYA83_000405"
     CDS             complement(21561..24239)
                     /gene="mgtA"
                     /locus_tag="IYA83_000405"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ8585870.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            24899..25336
                     /gene="ndk"
                     /locus_tag="IYA83_000406"
     CDS             24899..25336
                     /gene="ndk"
                     /locus_tag="IYA83_000406"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ8585871.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(25358..26224)
                     /locus_tag="IYA83_000407"
     CDS             complement(25358..26224)
                     /locus_tag="IYA83_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8585872.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(26458..28536)
                     /locus_tag="IYA83_000408"
     CDS             complement(26458..28536)
                     /locus_tag="IYA83_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ8585873.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            29029..30246
                     /locus_tag="IYA83_000409"
     CDS             29029..30246
                     /locus_tag="IYA83_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ8585874.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(30705..31328)
                     /locus_tag="IYA83_000410"
     CDS             complement(30705..31328)
                     /locus_tag="IYA83_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585875.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(31484..32881)
                     /locus_tag="IYA83_000411"
     CDS             complement(31484..32881)
                     /locus_tag="IYA83_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8585876.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(32903..33874)
                     /locus_tag="IYA83_000412"
     CDS             complement(32903..33874)
                     /locus_tag="IYA83_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8585877.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(33881..36181)
                     /locus_tag="IYA83_000413"
     CDS             complement(33881..36181)
                     /locus_tag="IYA83_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAQ8585878.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(36183..37508)
                     /locus_tag="IYA83_000414"
     CDS             complement(36183..37508)
                     /locus_tag="IYA83_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8585879.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            37739..38164
                     /locus_tag="IYA83_000415"
     CDS             37739..38164
                     /locus_tag="IYA83_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585880.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(38662..39363)
                     /locus_tag="IYA83_000416"
     CDS             complement(38662..39363)
                     /locus_tag="IYA83_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ8585881.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(39379..39819)
                     /locus_tag="IYA83_000417"
     CDS             complement(39379..39819)
                     /locus_tag="IYA83_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAQ8585882.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(39819..40394)
                     /locus_tag="IYA83_000418"
     CDS             complement(39819..40394)
                     /locus_tag="IYA83_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ8585883.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(40805..41311)
                     /locus_tag="IYA83_000419"
     CDS             complement(40805..41311)
                     /locus_tag="IYA83_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAQ8585884.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(41322..41801)
                     /locus_tag="IYA83_000420"
                     /pseudo
     CDS             complement(41322..41801)
                     /locus_tag="IYA83_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(41973..42674)
                     /locus_tag="IYA83_000421"
     CDS             complement(41973..42674)
                     /locus_tag="IYA83_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8585885.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(42782..44224)
                     /locus_tag="IYA83_000422"
     CDS             complement(42782..44224)
                     /locus_tag="IYA83_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8585886.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(44238..46103)
                     /locus_tag="IYA83_000423"
     CDS             complement(44238..46103)
                     /locus_tag="IYA83_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8585887.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(46224..47075)
                     /locus_tag="IYA83_000424"
     CDS             complement(46224..47075)
                     /locus_tag="IYA83_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8585888.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(47385..48098)
                     /locus_tag="IYA83_000425"
     CDS             complement(47385..48098)
                     /locus_tag="IYA83_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ8585889.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(48166..48492)
                     /locus_tag="IYA83_000426"
     CDS             complement(48166..48492)
                     /locus_tag="IYA83_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ8585890.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(48615..48842)
                     /locus_tag="IYA83_000427"
     CDS             complement(48615..48842)
                     /locus_tag="IYA83_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ8585891.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(49164..50417)
                     /locus_tag="IYA83_000428"
     CDS             complement(49164..50417)
                     /locus_tag="IYA83_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ8585892.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            50478..51218
                     /locus_tag="IYA83_000429"
     CDS             50478..51218
                     /locus_tag="IYA83_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8585893.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(51304..52062)
                     /locus_tag="IYA83_000430"
     CDS             complement(51304..52062)
                     /locus_tag="IYA83_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ8585894.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(52059..53087)
                     /locus_tag="IYA83_000431"
                     /pseudo
     CDS             complement(52059..53087)
                     /locus_tag="IYA83_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(53134..56274)
                     /locus_tag="IYA83_000432"
     CDS             complement(53134..56274)
                     /locus_tag="IYA83_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585895.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(56498..57382)
                     /locus_tag="IYA83_000433"
     CDS             complement(56498..57382)
                     /locus_tag="IYA83_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ8585896.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(57477..57914)
                     /locus_tag="IYA83_000434"
     CDS             complement(57477..57914)
                     /locus_tag="IYA83_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585897.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(58131..59042)
                     /locus_tag="IYA83_000435"
     CDS             complement(58131..59042)
                     /locus_tag="IYA83_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8585898.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(59499..59927)
                     /locus_tag="IYA83_000436"
     CDS             complement(59499..59927)
                     /locus_tag="IYA83_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8585899.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(59955..60593)
                     /locus_tag="IYA83_000437"
     CDS             complement(59955..60593)
                     /locus_tag="IYA83_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585900.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(60597..62633)
                     /locus_tag="IYA83_000438"
     CDS             complement(60597..62633)
                     /locus_tag="IYA83_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ8585901.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            62759..63424
                     /locus_tag="IYA83_000439"
     CDS             62759..63424
                     /locus_tag="IYA83_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8585902.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(64915..65787)
                     /locus_tag="IYA83_000440"
     CDS             complement(64915..65787)
                     /locus_tag="IYA83_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ8585903.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT        19185 a        13911 c        11161 g        21640 t
ORIGIN      
        1 ttttgaataa accacaggct tcacagcctc tctttatact tctatacgtg aagcatccca
       61 gtggcaaata aaaccattgc gattactccg accatcgtca caccgataag taaaatctta
      121 tctaagtgga tatgttgtac aggttcgttt tgttctttac gtgctttgag gtaaaaataa
      181 atacctggta agaaaagaac aagaacaagc atcacttcat taaaggctac aactagcatg
      241 gcccatactt gaaaagcact tgctaaaaac cctactaaaa attggaccca ctctttccgc
      301 tgataagaaa gcttcatttg gtacagccct aagaaaagcc acgtgaagaa aatcgctgct
      361 gtaccaagtg tataagcaaa gttatatgca ctgtcagtga ataataatga aagtaaaaag
      421 acattcgtac ataacgtcgt caatacgata gcataagttg gtgccccatt tttgttgagt
      481 tttccccatg ctttaggcaa aagtccgtcc tcagccatat tccccattgt ttcaatcggc
      541 aagattgtcc atgagagcca aaccccaact aaagaaatga tcaaaccgcc gttgatcacg
      601 accgcccccc atttaccaac catcatttca aatacatatc ccatagcagg ttgcgctttg
      661 atcgtttgca gctgattcat cgtcattgtg ccatatggaa gcatggaaac caagacgtaa
      721 ataatgatca aaccgaatat tcctagaatc gttgctttcc caacatccga ttgttttttt
      781 gctcgtgaac ctacgacaga agctccttca ataccaacga atacccagag catcaccatg
      841 atgcaactca atacttgact agaaaacggg atcggctgcc cattcgcatt caatatgata
      901 tcgctagaaa agttattaat gaattgttgg atcgaaaagg tgctgatagc tactacaata
      961 aataaaaata atggcactaa tttacaaaat gtgacgatag cattgacaaa gctagcattt
     1021 tcaactccac gattcactag ccatgcatat ccccataaga agatcgtact catcacgatc
     1081 gatgccacat tttgaccacc ttcgaatacg gggaagaaat aacctaatgc actcatcaac
     1141 aatgttgcga acgccacgtt tcctaaccag catgtgagcc agtaacccca accgctgata
     1201 aaaccagcga aacggccaaa accttcttct gcgtaactaa agatcccgcc ttttaaatct
     1261 ggacgttttt tattgatatt attgatcgat aaggagagca tcaacattgc tgttcctacg
     1321 agtacccagc tgaagatagc tgggccacca gccgatccgg agatgtccgt cataatacca
     1381 aatacgccac ctccaatcgc tgaaccaatc actaaggcag caagtggaat caaaccaatt
     1441 ccttttttct gttgctctgt atccatgtaa gcatcctcca aagtaaaacc gcttttatca
     1501 aaaagtataa caaacctttt ttatttttac ttaattaata aaaactttaa tagaaatata
     1561 attctaccga cttttttctc attatacaag caaagcgcct agtccctttc caaattacac
     1621 caccaaaaaa acttaaaaag atgctatact aaagggaaag aattggggga tgatccatga
     1681 acattttata tatatcgatt tcaggaaata cgcgtgcttt tgccaaacat ctagcagaat
     1741 atgctgaaaa aatgcatgca gaagatcctg tcaatcctga agttacttta aaagaaatcc
     1801 atgaaaatag tgattttacg aaagaaacag aaccattttt tactttcgtt cctacttatt
     1861 tagacggcgg gaatgggtta gacaacggtg acacagaaat cttgaccgaa acgatgcgcg
     1921 aatatctgga atacgaagac aatcataaac tttgccttgg tgtagtcggt agcggtaata
     1981 aaaacttcaa caatcaatat tgtctgacag ccaaacaata cgcacaaaga ttcggctttc
     2041 ctttcttggc agattatgaa ttacggggaa caccatctga tgtgacacgc gtttatcaaa
     2101 ttttggcaga aaaaaataaa taacacaaaa gaagaagctg aaaaataaat ttcggcttct
     2161 tctttttatg ttctctattg tttctattct tattaagctt tgacatccat cacaggtgct
     2221 tttgctttcg ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt
     2281 accaagacga tcatggaggt ttctttgtct gctgctttga ttgcttctaa gtcagcttct
     2341 gctacaagcg tatcaggtgt cacttgctgt ccttctgtta ctttcacatc gaatggcgta
     2401 ccgcctaagt caactgtgtt gatgcccatg tgaagcaaga tttctaagcc atttggtgtc
     2461 gtaatgccga tagcatgttt tgttgggaaa acagaactta ttactccagt aaccggggaa
     2521 tagatatgtc cgttttctgg ttctacaaag taaccttcac ccatcatttt ttgtgcaaaa
     2581 actgggtcac ttgctttatc taaagaaaca actgttccgt cagcaactga tgataagata
     2641 gtcacacttt cagctgaagc ttgaggtact gctttttcag gtacttcatt tactgtggct
     2701 gtttcggtcg ctagttcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta
     2761 gcaacgaagc caccagcgta agcagccaat aaaccaagga tatagcctaa ccatttacca
     2821 tcagagatca atggaatcaa agcggctcct gaaggtccaa tagcgattgc tccgacattt
     2881 ccgatcattc cgatcactgc cccaccgata cctccaccga tacaagcagt aataaatgga
     2941 cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctaa gattccgact
     3001 ggtaaagccc ctttgatcat ttctgttaat tctttgtctt ttttgcagcg gatccataat
     3061 gccaatgctg cacccacttg tcctgctccg gccatcgcaa ggattggaag caataaggta
     3121 cgaccagttt catcaatcat ttgtacatgg attggcgtca agatctgatg tagtccgaac
     3181 atgaccattg gcaagaataa tgtacctaga acgaagccgg caaatgctcc tccaacacca
     3241 agtacccagt tgatcccacc gaccatgccg tcagaaatga accctgctaa aggcatgatg
     3301 aagaaaattt cgatcagacc gatggcaatc agggaaagag ttggcgtaac gatgatatct
     3361 accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaataa ccaaactgcg
     3421 aagataacac ctaagatccc gccttgtcca gttgcaagag cttcaccggt aaacaagttt
     3481 ttcagtggtg cttctggatt catccctgtc agcatcacaa cagccccgat aactccacca
     3541 agcgttggcg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactagatag
     3601 ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttcctgcg
     3661 tctagtgttc cagctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca
     3721 gtgccgacaa atgcagggat cattggtaca aagatattag agatgtcttt taataccgat
     3781 ttgattttag agggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg
     3841 tttacacggt cttttccaga tgcagaagaa cttgctgtag ttgcactagc agaaggaagt
     3901 ttttctccta atttcacgcc tgctaagtca accatttcct gcgctacttt gttcactttc
     3961 ccaggtccga tgatgacttg gagctgttca tcctctacta cgccaagtac tcctggaatc
     4021 gcttttaatt ccgagacatt tacaagatca tcattgatga ccttcatacg tacacgagtc
     4081 atgcagtgga caatgctttc aacattgccc ataccgcctg ctccttgata aatttgtttt
     4141 gccagttctt ccaaagtcaa ttcgtttttt gccatggtct tttccccctc ataaggttcc
     4201 ttttacaaaa ccgtttgctt cagctaattt tgcttgagct gcctctttat cactatttgt
     4261 caaaatcatg acgatagcca gtttgacatt ttgatctgct tcctcaaagt acttttcagc
     4321 tgtttcgtag tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt caattagttt
     4381 ttcatttgtt ggtttgacgt cgaccatcaa gttattgaat actttaccga taccgatcat
     4441 actgatagta gaaatcatat tcaaaatcag tttttgtgct gttcctgact tcaatcgagt
     4501 agagcctgtc aaaaattctg gtcctgcatc gacttcgatt gggaaatccg catgctcgct
     4561 gatttctgct tgtttattgc aagaaatcgt tcctgtaaca gcaccaatcg attttgcgta
     4621 atctaatcca ccaatcacat atggtgtacg accgctagct gcgataccaa taactatatc
     4681 attttttgtt aaattcagct tgattagatc ttcttgccct aattcataag aatcttcggc
     4741 tccttcgacg gctacagtca ttgcttcttc tccaccagca attaggccta tgaccatttc
     4801 cggttctaca ccgaatgtcg gtacacattc agcagcatct aatacaccaa gtctaccact
     4861 cgttccagct cccatataaa tcaagcgacc acctttttta aaggattcaa tcgtgttggc
     4921 gatcactggc tcgatacttg gcagttcttt ttcaacagca gcggccactt tctgatcttc
     4981 tttgttcatg agttggactg cttgagaaat actcatttca tctaatccaa aagtttcttg
     5041 gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct
     5101 tttcttataa attgatagtt cataccagct ttgatttggt tgatcagcgg tagttcttct
     5161 ttgatgactt tggctactac attcactttt tcatccgctg gcaaatcata tttagttaat
     5221 tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga tcctttcata
     5281 cgcttagctg tatttcttac tgggattggc ggaatctctc gaaaacgcgc atctgcgctt
     5341 cgtatgacat ctcttgcatc atcttgccga ttcgtatgtt ttcctaatat atattcgtaa
     5401 aaaacttcat ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca
     5461 aattgctcct gaacgtgttc tgacagacct gcatctccga tataaacaat atccgtgtta
     5521 cttgctaata gatcaagagc acctgccaga ggatggatcc ctcgatgttc ttccaaagtt
     5581 ggtaatcctt gatacagtgg acctctcaac tctgtatctc ctggtacaaa gccttgtaca
     5641 aggaatcctt gagttttgag ccattgattt ttttcctgat accattcttt gtccaaccct
     5701 gtttcaagac gtggataata attatgccaa gcttcaatct gtgagaaatc agccgctgct
     5761 tctttcaact catcgatatc attaggcgtg atcgtgctgg cattcaaact gattttcaat
     5821 tgatgtgacc agtcggcgat ctgctgatta gaaatatgat aatccattct taaccctgtt
     5881 acaccaattg ctttcaaggg tgttagatct tctaatgaaa agccagctcg tttcaatgcc
     5941 tctcctgaaa tatcgaccat caactctagt ttattctttt tcgcttcgct tcctagttcg
     6001 atcaatcgat tttgataagc cgaaacatca tcttccggta tatgcatgga agtgaaaata
     6061 ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa
     6121 tcattcataa atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat
     6181 ttctatgact agagtatata tctaaagaaa caaaatttca atacttttgt taaaataatt
     6241 aaattttatt tctaaaaatt ggatttataa tgccaaaaga attgacacag ccacaaaaaa
     6301 ggcactgcta agcaaaagca gtgccttgaa acattttatt taaataacac aaaagtctaa
     6361 ttactcataa gaaattaatt ctgtattgtc taccatgaat gtaaagaacc cttcattagg
     6421 cattggatga aaataagcat tgatcactct tatcaaggca ttatgacaga ctaacaagta
     6481 gacattttct tcgtcttcca tgcattcttt caataaagga acgatccgtg cgtacacatc
     6541 taagaccgac tctccatttg ggaaacgtac agcaaacgct aaacgcgctt tttgaaagtt
     6601 ttcatctttg cttggcagtc cgtcataatc cccaaaatcc atttctacca atcgctcatc
     6661 agcggtcatc ggcaaattga gacgatcaac tactgcttgt gctgtatctc ttgctcgttg
     6721 aagaggggaa tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag
     6781 tgcctgtgcc tgcatctgtc ctttttcagt cagcggcaca tctgctcgcc cgcaaactaa
     6841 tccttgtgcg ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc
     6901 ttcgttgaca aagttctcct taaccatacc ttgttgtctt tgagattaca aggtttagat
     6961 tttctcaaga gaaataatct cagtagatac tttatctaga aaggtttgat catgttccac
     7021 tagtaacata gcaggtttca cgttcaaaat cagttgttct aattgttctt gattgaagac
     7081 atccaaataa ttcaatggtt catcccatat atagagctca gctggttggg ataaagattt
     7141 agccaattcc acttttttcc gttgtcccat actcatctgt tcgattttat tatgaaaaac
     7201 atctctttcc atcccaagct ttcggagatt attcaaaaat gcttgatagt ctactcggtt
     7261 tttcttcgcg aattccgcca atgtccctcg attgtcttca taattttggc gtacgtaact
     7321 aatggtcaaa tgtttgggct gggatttttc tcctacaacc ttgccgttga atgtccccag
     7381 aagataatgg ataatggatg actttcccgc gccgttagga cctgaaattg ccatccgctg
     7441 atgaggttcg atcgtaaaat gaactggctc gaataacaga ttttcatacc ctaattgaag
     7501 attttctacg cttaacagcc gtttatgatg agacacttgg ctattcatcg tcaaagcatc
     7561 gatatactcg atatctttta ataatttttc tttctcactg atctgggttt ccatccgatt
     7621 cacgattgct ttagatcgtt tcatcgtccg tgcagcatca gcgcctactg ctcctttatt
     7681 cactcgccta gattccgtat cgatgaatcc gacttttttc tttgttttgt ccccttctcg
     7741 ggaacgagac cattctgctt tttcagctgc tgttttcttt agcctgctga cttctttttt
     7801 caatttttca tttttagcta tttcaaattc atcacgaagt tttttctgtt cttcatagat
     7861 agagaaattc ccttgataaa gttccagttg gctcttttcg attgctaaaa catggtccac
     7921 tacttcatcg ataaatcccc gatcatgact caccacgatg aatccttgtt ttttcttttt
     7981 caagtaagcc gctacttgtt ttctgccaga gatatccaaa tgattcgttg gttcatcgat
     8041 taacgggaaa tgagtgtcat ccacaaataa gagtgccagt aggacttttg tcttctctcc
     8101 tcctgatagt gtgctgaatt ctctccataa gatttcagga tctgtttgca tcagttggag
     8161 ctctctttca atttcccata tctcaaaatc cataatctca tttaacacat aataggtcaa
     8221 acgctcttta tcttttgttt gctgcgggaa atagacaaac gcttgctgat ggatcacttg
     8281 cccttgataa gggagtttgt tttgcaggat attcagtaaa gttgtctttc ctcgaccgtt
     8341 tcgtccgata agtcctagtt tccattgtgt gtcaaagttt agattggctt gttcaaataa
     8401 taatgtgcct tggctgtcat agccgaatgt cagatttttt atttcgattt tagacattat
     8461 tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag
     8521 ggttggggca cacaaaaaga cccatacatc gaaagaacca agccaatccc gaaaatctgc
     8581 acactgctcc cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa
     8641 tttttcaatg cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa
     8701 attgtttttg tcaactactt cgggagaatg ttgaaaaatc tgctgagtgt ctgtcctaag
     8761 atttctgttt tttctaatgt ttgcctgtgt tccattgctt cctctaaatc gattggtcct
     8821 aattgaatgc ttaagacaat ggggagaaaa tcaaagagtc cttccagctc tcggtctcta
     8881 cttccgcaaa tagcgattat tggttttcga tatttttttg caagcactgc tacgccatat
     8941 ggaacttttc cgtggattgt ttgctgatca atccgccctt ctcctgtaat gatgacatcc
     9001 gcttgttcga tctgtttttc taatccagtg atttggctga ccaactcaaa accagagacc
     9061 atcttcgctc ctaacaaaaa aagggctccg ccaataccgc ctgctgctcc gcttcctggg
     9121 atgtcatcta aatccacctc ccaaacatct tttatctgct gagcaatctt ttcagcttgt
     9181 ttatccaaga aagctaacgt cgtatccgtt cctccttttt gtcttccaaa ttgccaagca
     9241 gctccttgtt gacctgtata tgggttggtc acgtctgcag cgatgatcac ttttacttta
     9301 gaaacaagaa cgttccttcg atccaaatga agttttctag tagataacaa aggatttcct
     9361 tctgtgtacc cttctaaaga agcacccaaa gattggagta atcctagacc accatcgcta
     9421 catccgcttc ctcctaaagt gacaatgatt tgttccactt cttgaagcag tgtatctttg
     9481 ataagctctc ccaaggcata actagaagca agtcggatgg tttgatccgt tggtttatca
     9541 agtaaatcta atcctaatac ttgtgcagat tcaatgactg cggtattttt tccttgccag
     9601 ttcgtcaata aataactgat ttttcgctga cgaaataaga gatccatccc atcaatcgta
     9661 cgccaggttc ccccgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca
     9721 atcggcacat tgatttttct tcctttacgg tcttcccaac catttagaac tgcctgattt
     9781 aattcaaatg aagaagcact tcctttaaat gaatcgattg ccgccaaaat atccatctat
     9841 atccctcact ttcttcttaa gtaagacact ttttttcact tttttcaaac aaaacaatgc
     9901 tattttcttc tgtttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa
     9961 catgaaaatc gcaatagctg gagccggagc aatgggcagc cgaatcggat taatgttgca
    10021 tcaaagcgga aatgaggtcg tcttgatcga tcgctggcct gctcacattg aagccattcg
    10081 tacaaatgga ttgattgctg actttaatgg caaagaagta gttgcaaaac tgccaatcta
    10141 ttcaccagaa gaaatcatcg aaagcaacaa acacgtggat ttgatcgtag ccttgacaaa
    10201 agcgaatcaa ttagatgata tgtttcgttc gatccaatcc attattactg agaatactta
    10261 tgttttgtgt ttactgaatg gattaggtca tgaagatgta ctggaaaaat atgtaccaaa
    10321 gaaaaatatc ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt
    10381 cacattatta ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt
    10441 tacaaaaaaa gttgtagaag tctttcaaga agcaaaattg aatccaatct atagtcataa
    10501 tgttcgttat tccatttggc gaaaagcttg tgtaaacgga acattaaatg gattatgcac
    10561 gatattggat tgtaatattg ctgaattagg tgcgcaaaaa gcggcagagg gcatggttcg
    10621 aacgattgta tctgagtttg cttctatcgc agcaaaagaa ggaatcacat tagatcaaga
    10681 agaagtgtac caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc
    10741 atctatgtac caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg
    10801 tgccgtttgg cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac
    10861 ccaattagtc catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga
    10921 atcagctgga tcagcagctg gttctcattt ttttaccccc atgaaagttc aaaaagaaaa
    10981 acacaattct catgttgcca taagaattgc gcttgtttat tttcatctat gattggttgg
    11041 cacttttatt tatttttaac agaggaaaga tcgtattcat attgttcata aatagcggag
    11101 tcagtagtga aaaaacgttt tgcccaattt tctgcatcaa aaaattccaa cagttctgat
    11161 gggattggtt gaaatggcgt atgcaaaaat tccggtaatt catctaaatc acgttcttga
    11221 agcaaaaaaa tattttcaga atgataaaac tgataatgag ctatggcttg attatctgtc
    11281 actagtttct tttgaaaata catagattca aacgttcgca aagtcactcc ttgctgccct
    11341 tcccgcaaca gttctagaat ccccctcgat tccccggttt ttttcagata ttcttcataa
    11401 gtaaggtatc caggttgttc atttcctcga agcggaagaa taaagtaatt ggtcgtaaca
    11461 cctctttttg taaattcctg cttatatttt tctgcccgtt cttttctgcc gttatttgtt
    11521 gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa aatccatatg tttcgaataa
    11581 aaggaactat tatgttttag tccaagttct ttcgcttcta aaaaatcaaa atgataaatc
    11641 tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttaaag
    11701 tggttccaaa aaaataacac gatttttgca ttggtaaatg gacgcacgcg tcgagccatc
    11761 tccatatcaa atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcga
    11821 gctgatttaa tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg
    11881 atggaaggaa aaaaataaag tgatttattt ttcgcaatta gcaatacgtc gtttttgttc
    11941 atctgttccg cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa
    12001 aagccagtaa aacgcttcct attattttta taaaaattta acatttctat gaaaatacga
    12061 tgaaataaat cattcttcca ttaaaatatg cccaaaagtt ttctgaatag ttgtaattat
    12121 ctgaaaattt gttatcatta gtcattatta ttattactat ttattcttac tatttattct
    12181 tactatgcat tttgattgtg aatttaaaag aaagaggtta tttatggaag ataaaaaatt
    12241 gaaaaaacaa cttagctccc gacatatcac catgctagct ttaggtggag cgattggtgc
    12301 aggtctattt aaaggaagtg gcgaagcgat tggaatcgcc ggcccttctg tattgatcgc
    12361 ttttttgatt ggcggcataa ttttatatat cgtgatgaaa ggtctgggaa aaattgtttt
    12421 aagtggtggt gatacgcacc acggattatc tggattgatt cgtccatatc taggtgctca
    12481 tgctgccgat tttaccgatt gggtctactg gtccatgtgg attatcaata tcattgctga
    12541 agctgttgca gctgcttcct ttttacagtt gtggttcccc cacatcccag cttgggtatt
    12601 tgtttttatg ttggctgttt taactactat catcaatctt tattcggtac gattattcgc
    12661 agagacagaa tattggctgg cctttgctaa aatcagtgtc attatcctat tgatcatttt
    12721 tgctgcttat cttgtcggtc agcaaatgtt gggtactggt gttttcccta cccttcaaca
    12781 attaactgat catggtggtt ttactcccca cggtatgaag ggaatcatca gttctttgct
    12841 tgtagttatc tattcttacg gcggttctga gttgattgct atcacagtaa gcgaagcaga
    12901 tgatcctaaa cgtgcgattc ctaaagcaat caaaggcgtc atgggaagaa tcatttcctt
    12961 ttacattatt cctttatttt tgcttttgat tatttttcct tggaatactt tagcaggcac
    13021 gaacgttagt ccttttgtga tggtatttga aaaaatgaat attccatttg cggcagatat
    13081 cgtcaatttc gtgattgttc ttgctctgtt ctcgtctatc aattcaggtg tatatgcttc
    13141 ttcgagaatt ttgttcttcc gcttgaaaga tcggaaaggt tcctctagaa aactagctgt
    13201 tttaaacaag catcaagtac cgcaacgtgc tgtgctgttt tgtaccagtg ttctttatct
    13261 aggtgtggct ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttctct
    13321 ttcctataca gtattattga tctggatcat tatcagtgct gcgtcctttg tactgacact
    13381 aaggaaagga acgttatggg acaaaggaat cagctttttt gcattagcag tcttatggtt
    13441 gattttcctt ggtatcctat tcacgaactc cattggggta actgtattga ccggtttgct
    13501 ttacctgttc atttatttca gttatcaaaa aaagaatgat gctttcgtct tgacgaatga
    13561 atgaaaccaa tagagaattt tattctattt atattgaata tctggagcta ttgaaaaaaa
    13621 ggagctgcga caaaaattgt tgcagttctt ttttccgaat aaacggtgtt caaaaaccat
    13681 ctgtgcagat taattgattg ttctccacgc tcatgacatg atagaagaaa ggaatgaaga
    13741 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat tggtgtttcg
    13801 acggtagtca tgtttggtat gatgtatttg aatgtttatg cgcttgatca tatcttcttc
    13861 agtcgcacac gtgtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgttg
    13921 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ctatgatttt agccgctagt
    13981 cttcttgttt ttgccggttc cttatttatg gtacgaagcc aaacaactgt caacgatacg
    14041 gcgtggatga aggcgatgat ccctcatcac tctattgcta tcttgacaag taaacgagct
    14101 gatttaaaag atcctgaagt aaaaaaaata gcggaagaaa tcattcaggc acaagaagaa
    14161 gaaatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc catccaaata
    14221 aaaaaagagg atgttccaga agtgatcaac ttcaagaaat aagaagaagc tacttctgga
    14281 acaccgtgaa tcagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    14341 caaaaatagc ttctcatatt tttcgtttgg ttaggcttat taccgcaaat gcaagctttt
    14401 gaacaccgtt tattcgtaaa agggagtgtg acaaaagtct tgtcacactc cctttttacg
    14461 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttctt
    14521 tgagatcccc caacctgtaa aggcactgac catcgaaaac acaggtgaaa gcaaactgaa
    14581 aaacacgaat ggcaagtaag tgagtgtagg aatgccaaat gtacttgcta cgaaacttcc
    14641 ggcgattccc caaggcacta agtagttgat tactgttcca ccatcttcta gtgttctccc
    14701 taatacagtt ggatcaaatc ctgctttttc gtaagtttct ttaaatgcat taccaggcag
    14761 gataacagat agaaattgtt ctccaataaa gatatttact ccgatgcttg tcatcaatgt
    14821 tgtaaaaatc aattttgggg tgttcgtcat ctttcttgat actcgttcca taacggttga
    14881 gatcaaccca aattcgatca acaaaccacc taatgacaat gttagaataa tcaatgacac
    14941 agttggcatc atgctttcga ttcctccacg gctaagtagt gaatcgattt gttggtttcc
    15001 agtatttgaa acaaatcccg attcgatgac ccctgccaac ttcgttaagg aagtttgtgg
    15061 gttttgaata aagatcattc ctactgctac agcaatattc aacaaaatcg tcaaaattgc
    15121 cggtaccttt ttccatgcac acaagagcat taaaaataga ggaatcagtg accagatcga
    15181 aatagtaaag tttgcttcta aaatatcagt cacttcacga atagatgaaa gacttgcggc
    15241 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccggaacagt
    15301 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctaa
    15361 atttgttgat tcggataatg gagacatctt gtctccaaaa acggcacctg aaacaatggc
    15421 tccaacaaca agggctggat ttagtcctag tgttgtccca ataccgaaaa atgcaatccc
    15481 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacaaacaac
    15541 aaatacagaa gggacgaacc actggacact aatcaattta aatccaatga ccatgagggt
    15601 tggaataatt cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    15661 gattggaatg atccccggtt tgatgccatt gatgatccct tcgtgcagcc aatctgtcgg
    15721 gtattttttt atcactgcgt acaacatgac cattccgata gctgccaaaa tcgtgatatt
    15781 aggtgaaacc tttaatccga caacgccaat cgcaatgcct aaaacaatga ccatcaatac
    15841 aagtgttgct tctaaaaaac ttggtgttcc ttttttttcc atgataatct ctcctttttt
    15901 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccaca attcatccgc
    15961 gattcctcct ttataaaaat aaaaagacct gctttcaact aaggacgaat gactccgcgg
    16021 taccacctta attaaaaaca gatctgtttt tcactttcaa acgattattc aaatgtatgg
    16081 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    16141 acaaaacacc taaccactac tgcgtgaaaa gttgacttgt atgaagttta ccttccataa
    16201 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    16261 tttcgcgcac aactttatct ttttactaga aagtagcgcc ctcttgtttt tttcttgcta
    16321 ctatttcttg atgttcttgt ttgatttttt ctaatagctt gggttctctc aataaatcta
    16381 gagctgtatt ggcaagtaat tccgctgcaa tcgcaataga agcaagtcct ttttcacttt
    16441 tagcagcagc tttgaattct tctgtatgtc cggcaatata tgtctcagag attgctacag
    16501 taggctgtat cgttggaata acttggctaa catttcctac atcagaagaa cctagacttg
    16561 ttctttgttc cgtttctatt tcttcatcag gaacccctgc ttcttttgca tgttggaaga
    16621 ataagtcatc gaaagaaggc gttacgatga catcatcgac tgcattttgg aacaatccaa
    16681 actcaaaagt cgtacccgtg cttaatgcag cacctcgtac gatattttcc actttttcat
    16741 atactttgtc taatgtttgt cgatttcctg cacgtaaata gaaacgtcca gcagcgtagt
    16801 ctggtatgac attggccgcc acgcctccat ctgtaatgat tccatggata ttgacatctt
    16861 ttggcaattg caagcgtaga gcgttgattc cgttaaacac ttggatcaag gcttccaaag
    16921 catttactcc tttttcaggt gctgctgctg catgagaagc gacaccgtaa aatttgatat
    16981 cgactggatc atttgctaaa gattctgtcg ttttcccata acgaaaagct ggatggacac
    17041 ataatgctgc atcgacatcc tcaaaaaaac cttcccgaac gaagcttccc ttagctgatc
    17101 cattttcgcc tccttcttcc cccggagtac catacacacg aatttctccg ccaatctcat
    17161 caatgaattg tttcactatg ctggctgcta atacggagta agtaccaaat aaattatgtc
    17221 cgcatgcatg accaatacct ggtaaagcat caaattctgc caaaaaggca attgtcggac
    17281 ctggtttatt ggaaatataa cgtgcatcaa agcctgttcg gtgcccagct acttcttttt
    17341 ctacccagaa cccttctttt tccaattgtt tgattaaagc tgctgaggaa taaaattcat
    17401 aattacttac ttcaggatgg tcgtggatag ctagtgcaag tgcttgatat tctggtaaac
    17461 ggtattgaat cagatccttc aattgatttg ttgagagtgt ggttgtcata aaaatttcct
    17521 cctatgctag tcgttttttc ttattttctc ccgaatgttt cccacgcagg tacgcttgaa
    17581 ccttttgatg ttttttcgat tactttggct gtttcttctg tttgataggc atctactatt
    17641 ttttgatacg ttttattgtc ttcatcttct tttcttgcaa cgatgatatt gacataagga
    17701 cgtgcatgct catcgactgg ttctaagaag attgcatcag aagcgggatt aaatccagca
    17761 tctacagcta caccgctatt gatgactgaa atatctacat ctgacaaagc acgtgctgtt
    17821 tgggaagcat ctaactcaga aatgtctaga ttttttttat tctcggtaat atcagaaacg
    17881 gtaggcgctt ggccttttgc cttgtccact ttgatcaatc cagcagtttg caataacaga
    17941 agagctctgc cgccattcgt gacatcatta gggattgcaa cttttgctcc atctttgata
    18001 tcagccacat cttttaacgt attggaataa attcccaacg gtgcattaac cgtattccca
    18061 atcgatacta aatcagttcc gtgttcttca ttgtaattgt ctaaaaagaa ttgatgttgg
    18121 aaggaattca aatcgatcga tccatctgct aaagccgcat ttggctgatt gtaatctgta
    18181 aatttcacat attccaaatg gatgttttcc tttttcaaac ggtctttcac atcgtcccat
    18241 acatctgtat cttccccaat gatccctagt tttacggtcg tttctttttt ttcagttgta
    18301 gctgcttttc cttggctgca tcctgccaac caaataattg ctacaaatgt aatgactgca
    18361 aaacgaataa tttttttcat tttttctcct ccattttcaa tgtgtgactt ttttaatcag
    18421 ttgattactg ataaattgac tcaaaaaaac taagaccaga atcaataatg tagcgacaat
    18481 cgttacatct gtttggaatc gattatatcc tctcgatata gcaagattcc ctaatcctcc
    18541 ggcacctact gctccagcca tggtagtcag tccgatcaca ttgatgatcg tcaacgctga
    18601 gacccgaatg ataccaggta acccttctaa caaataaaca cgtaaaatga tgccgattgg
    18661 gctcgtgccc atcgcttctg ctgcttcgat caccccagga tcaacttcta gcagtgcatt
    18721 ctcgatttga cgggcaaaaa acgggacaac gccaaaaatc aaagggacta aagctgcttt
    18781 ggcaccaatc gtcgttccaa ctaagaaacg agtagtcaaa gctaaaagag aaagcaagat
    18841 aatgaaaggg atggaacgaa tgatattgat caacttatca agtatttcga acagcagcct
    18901 gttagctagt aatccatttg gacgtgtcac tactaacgtc acacctaaga tgattccgat
    18961 gatcccacca atgaatgccg tccaaaaaga catatatagt gtttgctttg tcgcttcgat
    19021 gaattcatcc ggaatcaaat agacattcgg caaatatgtt ttaaaaaatt cagtcatttt
    19081 agtttcctcc ttcgatgact ttgatacggt tatctaattc atttttttgt aaaatagtta
    19141 cttttacacc agatttaatc aaataatcca atgctttttg ccgctgtttg gtttctccag
    19201 ctaaagtaac gatcaaactt cctaagggtg tttcttgtaa tagctctaca ttaccgtata
    19261 agatattcgc tgttacttgg tacttgctgt aaagatgagc gattagaggt tcgttcgtct
    19321 ggttgccgat ataacttaat tccaccaacc atttatttgc aatctgatcg gcaaaagcag
    19381 catgggcact gattgtttcc aatgcttggt caaggtgtgt cgctgtccga ataaaatctt
    19441 tagtcaattc ttcttcagga tgactgaaaa tctggatgct gctacctttt tcaatgattt
    19501 tccccgcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    19561 tgatcaggac gattgttaga cctagctgac gatttagttt ttttaacagt gcaaggatct
    19621 gaagggtcgt tttcggatca agtgcactag ttgcttcatc gcataacaac actttaggtt
    19681 catttgccag agctcgtgcg attgccactc gttgtttttg cccccccgac agttgtttag
    19741 gatacgcaga tgctttttct tccagaccga caagttccag tagttcattc actttttgac
    19801 gacgttcctg ttttgatttt ccagaatact ttagagaaaa atctacgttg tcaaaaatcg
    19861 ttcgactttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    19921 gcaattcttt tggagagagc tggcttaaat tcgtctgatt gacgatgact tctcctgctg
    19981 ttggtttctg caacaaattc atcacgcgga ctaacgtact ctttcctgca cctgagtaac
    20041 cgacgatccc ataaacatcc cctttttcaa tcgttaaatc tacagaatct actgcctgta
    20101 tctctttttc tttttgtttg aaacatacag aaatatctcg caattcaatc attaacattc
    20161 ctccttgtct ataaaaaaag catcctcaag aaaatgaaaa tagtcatttt cttgaggacg
    20221 cctttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt
    20281 accatcatac tctagcaaga tatcggtcgc agccgtcacc gccttacatc tatgctcggg
    20341 atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag
    20401 ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa
    20461 aaaatccttt tactaacata aactatgtca gtaaaaggac gacgtatcgt ggtaccacct
    20521 tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt
    20581 ttaacggtcg aacatttccg aatttgccta cttatcgaca aattattctc caaggccatt
    20641 ttcagctttt cttacttgac tcttcacacc agccagagcc tctctgcaaa gtttccaagc
    20701 ttactttcct tctcaacgaa atcaatattt tgttgttggg aataatctac aggaggaaaa
    20761 ctgaaaagtc aagcatgaaa tccaaattat tttttctata tttatttttc tgttttttta
    20821 tctaacattt caataacagt tcgaagatct tttccttccg caagaaggtt caatttttcc
    20881 ttctcttcga cgctataatt tcgaacaaaa ctggaatcgg gaatcaaaac aacgcctagt
    20941 cctgcacgtg aagcggctgt cgctcctact aaagaatctt ccactgcaaa agctgaaaga
    21001 gcttctaatc ctgttcgttc caaagctgtc agataaggga ctggtgaagg cttgttctcc
    21061 gatacttcat ctccgaacgt aaacgtatca aagtaatcgg ctaattgaaa gtgatccaag
    21121 atatctgttg ctcttttcgt aacagttgtc gtagcaatcc ctagtttcat atgacgttct
    21181 tttaagtctg tcaatatttc ttttgcatag ggtttcaatt ggatagctcc acttgtcagc
    21241 tgttgataaa aatattcttc acgctttgct cggacttcct gcactttttc aggactgcct
    21301 gctgcctgca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg
    21361 atttctttcg ggatatgaat atcaaattgc tggaatcccc aaagccaacc atcccgatac
    21421 gttctttccg tatccactaa caaaccatct aaatcaaata ttgcaccttt catctataca
    21481 ttaatccttt ccaaaacaat acacctagtt tagcatgact gatccaaaat gaacagaagc
    21541 atagagccca gccgcatcac ttataagagt tcttggaatc gacggatata gattttcttc
    21601 acagctgtca caagcgccag ataagcgacg actgttataa aaaaccataa ccagtaattg
    21661 ccagggagcg gcatcaagcc taactcagca ccaaatcctg tgaatggaag gactgaaccg
    21721 acaatgattc ctaatgtagt gataaccagc atggcaaatg ttgcattgct ttggataaat
    21781 ggtattttcg gtgtacgcaa tgcatggagt actaatgttt gtgtccacag ggattcgaca
    21841 aaccatcctg catggaataa tgcgataaaa atcacttgtt gtgagctatc caatgtatgg
    21901 aaacttccac caactacagc aggacaaata acaaaataca tcaacaagta agtagtgata
    21961 tcaaaaatcg aacttgttgg accaaaataa accatgaatt ttccaatact agatgcattc
    22021 catttctttg gctcatccaa atattcttta tccatccgat cccatggtaa ggaaatgcaa
    22081 gaaacgtcat agatcagatt caaaaataga agttgcaaag gcaacattgg taaaaatggt
    22141 aagaacgtac tggcaatcag tacggaaaac atattgccaa aattagaact tgctgttgcc
    22201 ttgatatatt tcatgatgtt tccaaaagtt tctcttccag aaagaaggcc tctttccaaa
    22261 atcgtcaaat ctttttctaa aaggatcacg tctgctgatt cttttgcgat atccacagct
    22321 gtatccacgg aaataccgat atcagccact ttcattgctg gagcatcatt gataccatct
    22381 cctaaaaatc cgacagtatg cccattttta cgtaaagctg tcgtcaatcg tgctttttgc
    22441 tgaggattca attttacgaa tacttcgtgt tcttcagcta cttgcgctaa ttcatgatca
    22501 ttcatttccg agattttctc accagtaatc aattcattga tttctaggcc tacttgacgg
    22561 cacacagacc gtgtgactaa ttcattgtcc ccagtcagaa cttttacagc tacaccatgt
    22621 tcttttaaag ctttcaacgc ttgttttgtt gtttcttttg gtggatcaag gaaagcaaga
    22681 taaccaatca gaaccatgtc tgactcatct ttgactgaaa attctccaac aacgcttgga
    22741 ttggtctttt gtgcgacacc aatcactcgt aagccgtctt cattaagtga ttgtactttt
    22801 gcaagcaccg cttcttttcg ttcttttgtt aatggtgcga cagtaccacc aagatcaata
    22861 aatttagaaa tgttcaacat ttcttcaatc gcgccttttg tgatcatctg tgttttgcca
    22921 gccggatcag cgactacaac actcatacgg cgacgttcaa aatcaaaagg aatttcatct
    22981 actttttgat aattgattgt atctgtatct aaagtttctg ctgcttcatc aatgatagcc
    23041 acatctaaca aatttttcag ccctgtttga tagtagctgt ttagataagc atggcgcaaa
    23101 acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat tttgtcttgt
    23161 gtcaatgttc ccgttttatc cgtacataac acatcgatcg caccaaaatt ctggatagca
    23221 tttaaatttt tgatgactgt tccttttttg gccatcacac ttgctccttt gaccaaattc
    23281 gtcgtaacga tcattggaag catttcaggc gttaaaccaa ctgccacaga taatccaaaa
    23341 agaaaagctt ctaaccaatc tccttttgta agcccattga taacgataac tgctggagcc
    23401 atcaatgaca taaatttgat cagcagcatg gatgttttgt ggatgcctaa ttcaaaactt
    23461 gattggatgg gtttttcaga tagcgtttct gctacatgcc caaatagtgt gtcatttccg
    23521 acagaaacaa tgattccttc cgcgcttcca ctgatgacat tgcttcccat aaagacaaga
    23581 ttttcataac ttgtttcact ttgctgatcg tcttcacata ctttcgcatg cttttctact
    23641 ggatagcttt cccctgtcaa tgctgcttga gaaatgaata gatcctttgc ggacaataaa
    23701 cgcatatccg ctggtatcat atctcctgct gaaagccgaa caagatctcc acagacgatt
    23761 tcttccatag gaatctctgt atactcgcca tttcgtttga cagctgccgt tactttcaca
    23821 agtgatttca gtttttcagc tgcttgatta gaacgcacag actggatcaa cgtcatcgtc
    23881 ccactgacga taaccatagt gaagataatg gctacaccaa agagatcttt atctcctgga
    23941 gcggctaata cgtattccgt aatgaatgaa ataattccta atgcaatcaa cactaatgta
    24001 aagggtgtga tgtatgcttt caatatttct ataatgaatg gtgttttttt gccatatgaa
    24061 atcgcatttt caccataagt ttctcttgaa cgctcgattt gttcttctga caatccattt
    24121 ggtgatgcat ggaacaattc aaataattct tgcttcgttt ttccagcaaa agctgcatag
    24181 attttttgtg tatctttctt tttttccatc tcgatctctc ctttgtgtcc aaaagacagc
    24241 tgttctcgct gtttttggtc ttttgctatt catcggatcg atagagaaaa tgctgaaagg
    24301 ggctgagaac agatagataa tagccctggc ctttcctcta aatccttgtt tttcgaatga
    24361 ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga cttccaatca
    24421 tttttcgaat agttattttc ccaactcgct cacctccctt tttgtttttt cacctatggg
    24481 atttgcggca acaaaaaaac ctctcgccaa atactcagcg aaaggtttga aaaagcaaat
    24541 caaaaaaagc ttctcagatc tcatcgttga gttttgacac tgtacaacgt aaaaagcaga
    24601 tatctgcttt tagctacctt atgaaaagcc ttatctcgac aattcctgtt ctacccattg
    24661 gcgtctctcg acgtttttgg gcagtagcct atgtttgtat aggagcctca cctaacaaat
    24721 cctattcggt tttccctcgt taatataaca actaaaaaag aggatgtcaa atcaattttc
    24781 catgtttttc atactttttt aaaatacacg cgttatattt cctgtctcac gcagttttat
    24841 ttgtctcatt ttccacctcc cgctataata gaattatagt aggaaacgag gtgctgttat
    24901 ggaacgaaca cttgttatta tcaagccaga cggcgtaaga cgccatttgg tcggaagcat
    24961 catccaacgt tttgaagcaa agggactggc cattgctgaa atgaaatttg caacgatgac
    25021 acctgaacta gcaaaagaac attatcagca cttgactgaa cgtagttttt ttgatgagct
    25081 tatcgattac atgacttctg gtccggtcgt ttatcttgtg ctggtcggcg aggaagtgat
    25141 cgatatcgtc cgtaagatgg tcggagcaac aaaaggggcg gatgccgttc caggtacgat
    25201 ccgaggagat tatgcactgc cggggactga aaacatcatc catgcttctg attcccgtga
    25261 tgctgcaaca aaagagatcg cccgcttttt cccgccattc gatacaaaca aaccgtctga
    25321 aaaagcggcg aagtgataca aatcctaaac tgttggtcta actgctgggt ttagcaatgg
    25381 ccagcagttt tttctctaaa gaattccttt gattcttccc taatgtatat tcgatcactg
    25441 cgaaaaactc ttggtgattt tccgattttt cttcccctaa agcagcagaa atcagtcgtt
    25501 ggtaataatc cgccatcaat gtttcataaa tcactgtact tctttcttta tgcgctaaac
    25561 tccgtttcat atcttcattc acgctacgtg ccttttttaa gcgaccattt tctatataaa
    25621 acaaagtcaa gttcgtcaaa aaagtaaaga gatactgctc gtgattattc agttgcgcga
    25681 tccgtcgcat tctttttttg attttcaaat aattattctc aataaaacga tcactgaata
    25741 tccaaggaaa atctgctaga atagatactt caaattgccc atactctggt acttgcatca
    25801 aatattcttc aatgatacga cattcataac gcaaaaattc aaattccgtc tcagatactt
    25861 ctgttttttt ggacatgtgc agattcaaaa ccaaatagta cagatcttct gttttcttat
    25921 agtgcctgcg gttattagca atttctttgt aaggatcaaa gctgtcttct tgaatccgat
    25981 ctagcattcg tctttttttc tttaattttt ttggctcatc aagctgattt cgtatccatt
    26041 catattcggc aagtgtcatg ttcattcgct ctaaaaatga tttcaatgtt ccaaacgata
    26101 cttttttcac tgaatttgtc tcgatcccga ctagcgaagt tttagaacag attccttccg
    26161 ctaattgggt ctgtgtcagc tttttgttga ctcgaagctt cttaatcaat tctccatcat
    26221 acataatagt tctccttttt taattgaaat taacccttgg cttttccaga atgcttgcca
    26281 ataagaggaa tgattatttc aatttaaatt ttccgcattc aataaataga tacgttaaaa
    26341 ataaaaaata cacgaaaaaa ataaaaaaat gtttttctca taaaaaatac caaccttcaa
    26401 gcatgagact gctgtctaat ccctgaaggt tggtttgatt gtttattgat cattttttta
    26461 ctgctccgca ttggttcctg ttactggata taggatagtc gttgttttat tgccgttttt
    26521 tgaaaagaca ctggtcgaat gttcaccttt ttgcttttcg attgcttcat tacgttccgc
    26581 ttcgttttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg gcacataaaa
    26641 ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct tgactttttc
    26701 ctgatctttg gagatttcct cgtcgattcc tgctgctttc gtatcgatga tttctcctgt
    26761 ctgattattg taagcttttc ctcctaggac ggtgtatttg ggcgtcacga agttcccatt
    26821 tcgaaaagca actacttgat cgtgctgaga agataataag tctgtaccaa aatgaagata
    26881 atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga tttctccacc
    26941 atattcatga ttgactgtcc ctttagtgta accaggcata tggatcatca atggcactcg
    27001 ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa gagcacttgc
    27061 taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca caaagattga
    27121 attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg attgatccag
    27181 ataatgggcc gttcgaacat aattatctac tgtgctattg ccagtattca aagatggaaa
    27241 gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa gaaatttcgt
    27301 gtaaaacggc tgttgtagat gttcaagata ttgggcagat tctctgaata aatctttatc
    27361 taatatccca taacctaacg tttcatcgga ttcatcaaaa taagatcgat caaagaaatt
    27421 gtcataccct aaatttttat atacatgatc tctattccag aaactagcta cattcccatg
    27481 gaaaacagca cttgtatagc cttctgtttg accaagggtc gctggtgctg cttgaaagac
    27541 attatctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg tgccagtttc
    27601 caacatgttt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa
    27661 actgatcgtt gcttgattct ggaaaatact atttaaaaac ggcgtgactt cttgtccgtc
    27721 taccttcata ttgatcaaaa actgttgaaa actttctaaa tgaaggacaa tcacattttt
    27781 tcctttagca atcccaaatg tttcaggatt tggctctgca taatgtttct ttgtatagtc
    27841 taaaacttca tcaattccat tactgcttgc atgcgcgcga acactgctag tcattcctgt
    27901 tttgattccg tcataaatag tgtacgcatc gattcctaga tatttgacaa tataggaacg
    27961 gtcaaatgtc cgctgtagca attggggacg gttggcttcg ctcaacgcta aatttcctga
    28021 aaaaatcaat cctgaaagac aggtaacagc gatagccatc ggtttgctga cagggttgct
    28081 tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca cagcgatatc
    28141 caaccaatag atgacatcat ggggtttcat caaagaaaag gagcttcctg acaacccttg
    28201 tgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga attcccgata
    28261 aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca aaagaatggc
    28321 taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata aggtagtcgc
    28381 aatcgggttg atccacaaga taaagtactg gatcgtacct tccacaccta atgaaaaatc
    28441 tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca aaacgattgc
    28501 taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc tctattcaca
    28561 ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat
    28621 ttttttattc tttctaaaga ttattccatt catttttcac agcataaaat cgtttcggtg
    28681 taagcggtta aaatagtaag gagataaaaa tcttgtggtg accgatttac tctatagtat
    28741 tcaaaagtag tttctcggta ttcgtacaaa aaaaacatac taagattaga ggggaacatc
    28801 attgacgaat gatgttctcc tctattttct atttttagat tatagtgaag agtgaaagaa
    28861 agttcgaaca aagccttgag gactacgagt ccctatcaaa aaccgaactt ctttactgtc
    28921 attttagaat caggtttacg tatgttgggg ccatgagccc gtgtatagga aattggaatc
    28981 gaaatgaagc agtaaagttc tttcaacact taaaaaggag gaaaacaaat gccttacatt
    29041 cttgcattag atgtttcaat gggacacagc tacagtgtcc tatacaaaga cgacacctgc
    29101 ttatttgaag acgagatcga acacactcaa agaggatttc atgcattact cgaagagatc
    29161 cagcacttgc ctgaacgtcc attgattgtt tttgaagcaa cgggtattta ttccagacca
    29221 gtcgaaaagt tctttcaaga tcatcacttt tcttactgtt tgttaaaccc gttagaagcg
    29281 aaaaaacaga tggaagaaaa caccttacgc agttggaaaa cagataaatc ggatgcccat
    29341 cggctcgcac agactcatct taaaaatcaa cgtcaaccaa aagaagtaca aagcaatttt
    29401 tacttagaga tgcgcgattt agcccgtttt tatcaagaaa ttgagaagaa aatcactcga
    29461 ctgcgcatgg atttacacaa ctgccttcag ttgacttttc cagagcttga acagttcttt
    29521 tcaaacaggc tgacccctta cgcattgaca ttgatccgtc tttttcccca tccagatttt
    29581 gtattggcat ctactcggac aaaaataaaa aataaactga tcaacgaaac acgggaaaaa
    29641 atctccgcga atcgtgccga gcaaaaggca gatcaaatca tccactatgc acagtgtgcc
    29701 tatccagctg ttgaaaaaga cagtattcac tgtcaaaaaa cgatctatta cgccgaactt
    29761 ctccaagatt tactcgaaca aaaaaaagca ctggcgacac aaatgatcaa aaaagccgaa
    29821 gcctctcctt gctttttgtt gtaccaaacg ttccctggga ttggcgcact cacagccgct
    29881 ttattgctcg gtgaattagg agatatcaca cgatttaaaa cccataaaca attgaatgcc
    29941 tttgtcggaa tcgacatccg acggtatcat tcaggtaagt atactggcca agaccgcatc
    30001 aataagcgtg gtaatcccaa agcacggaaa attattttct tcaccattcg aaatatgatt
    30061 cgccagcaac gcgcagctcc taatcatatt gtcgattatt actacaaatt aaaaaagcaa
    30121 cctatcccta agaaagataa ggttgccaca gttgcttgta tgaacaagct tctgaaatgt
    30181 atgcacgcta tggttagggc acatacagag tacgactatg cgtacgcggt ctcagtggac
    30241 cattaactaa accacttaca gtataccact tctttgccca gatcgaaaat tttttacgaa
    30301 cggggcttta tttagtgtgc cttgattttg tcgctttcgt ttacgagcta tttttcttgc
    30361 accttttact tgactaatcg taggaaagag gctgtgacaa aattcttgtc gcagctccct
    30421 atttcttgat gcaggacagc tttttcacaa cctctccact aaacggtgtt caatcagctg
    30481 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctattttccc gtttttttct
    30541 ctaatactca ggtcaaaccg ctgatttcac aacctctgat tcacggtgtt ccagaagtag
    30601 cttcttcgga aataagccga aattttacaa aaacttgaga agcacttttc gtaaatttct
    30661 tcttatttct tgaagctgac cacttctgtc acaacttctg tttttcattg tccataggtt
    30721 tgatattgtc cttctgatga ctcattcgta atgattgagg cttgattttc ttcatttccc
    30781 gttatcgttt gtccactgcc agaatctgaa gggcttagtt ctgtcgactc gtttttttga
    30841 gaagaagcag ttgttttctt gacttgttcg ttggtctttt tccattccgc tagtcgatcg
    30901 gccaccactt ttttgtccac tttttcagaa taagctgtaa ccacttttcc atttttcaaa
    30961 cgtgctacct tgtcgccatc atgagtcaaa agcaaaacga ccaaatgttt gtctttgaat
    31021 tgatattgtc tgagagaaat cgcactaagt acgtcatcga tattgataaa ttgattttct
    31081 tcttttcctt ttggcaaata gatgtccaat tcaccttttt cgtaattggc agttaattta
    31141 tattcctccc accaataagt tgagaattct tttttgaaat tatctactga ttctttgctt
    31201 gctacgattt tttctgtttc tttctgagca agatctgctg tcatttttcc tgaaaatgct
    31261 gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt
    31321 ttcctcatgc cttcctccac tcctatttaa ttttgactat tctattatat acaaaaaatg
    31381 aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat tgaacttctt tttcctgaag
    31441 cggtaagaac gggaattttc atacattctc tcttggctat tctttaagct tcaaatccat
    31501 ttctgtctga tacatctttg aaccaatgac ccgatttctt gatcgttctt tttccatctt
    31561 gtgctaaatc tactgaaata aaaccgtaac gattcttata ggcattggtc cacgaccagt
    31621 tatccatgca tgtccacata tgataaccta aacaattggc accttcttga atggcttgat
    31681 ggacatattt cagatgagat tgaacaaatt cgatccgata atcatcgtgg atcataccat
    31741 cttccttgat aaatcgtgct tcattttcta cacccatgcc gttttcagaa ataaaacagt
    31801 cgatattccc ataatgatct cgtacattga tcaaaatatc atagattcct ttttcataga
    31861 tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg tcgaagtaat
    31921 cctcaggcat aggaccgttt ttagtatcga ttggtgtttc ttttgctttt attcggcgtg
    31981 gttggtaata attaattccc aaaagatcta ctgtgttttc tttaatgatc gtcaaatcat
    32041 ctggatgaat ctccggcatt agatcgagtt cttttattct ctcaatcaat tcactgggaa
    32101 actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct gctattcgtg
    32161 ctgctttaac atcttcggga tttgattcgt ctcttggata actcggtgtc aaatttaaga
    32221 tgattccgat cacaccgctt tgatccattt catggtaagc tttgatggct aatgctgaag
    32281 ccaacgcttc atgataacca acttggattg cctttttcat atctgattct tccggataat
    32341 gccatcgata caaatagcct gcctcgactg gaacgattgg ttcattatgg gtaaaccatt
    32401 tttttacttt cgagccaaac aattgaaaac aactttttgc gtatttttca taggcttcaa
    32461 ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata tcgaaatgat
    32521 acagattaac aaagggttcg ataccgtttt caatcaactc atcaaaatag tgatgataga
    32581 aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg ctccattgga
    32641 tagaggttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc tcttgatact
    32701 tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt tgcggttcgt
    32761 ttttgtacca ataatcaaaa atattctctc cttttccatc gcctgaaatt cttccttctg
    32821 tttgtggacc acttgctgca gagccccacc aaaaattttt tggaaattga tatcttttca
    32881 tcaaagcacc tctttttctt ttttagttga ttccatgtat gatattttcc atcacaatca
    32941 ctttttcatt ttcttcctct tcttttttga ttcgtttcaa cagctgttct gctgctcgtt
    33001 ccccgatttt atctgttggt ggcgtgatcg tgatttttgg aaggatactc agatcgtttt
    33061 tatctaaata atcatagtga ctgaacgcaa tatccttccc gattctgaca ccttcttgat
    33121 tgaaacattc gatggcacca atcgacatgt tgtaattgct gacaaaaata gcagttgccc
    33181 ctttttctaa taatgctttt gccccgtaat agccgctttt tctagaatag tctcctgtaa
    33241 agatctctaa atggatagac ggatcctttt gtaccgcttc tttggctcca gctaaacgtt
    33301 ctcttgctac gtaatctgtt tgagcggcag caataaatcc tattgtttta tgtccttgtc
    33361 cgccatgatg agcaatcact tttgctacac tttctcgatc attcactact attgtatcaa
    33421 tacctgctgg cccacccgga caattcaatg cgacaactgg gatactcatt tttgtcagca
    33481 tttcaaatcc agaccactct tcttctgata agaagacgat caatccatcg atcgcatgct
    33541 tcatcaggta ttcgattttt tgttcgattt tttctggctg atcggcaaac ccgcttaata
    33601 ataagctata cccattttct tcagctactc tttcgatccc tgaaacaacc tccgaaccaa
    33661 atcggttaga tatcccgttt atcaacagtc caatagagaa acttcgatta ttcttcaatc
    33721 ctttagcaat gatgttttct ttatagtcca acgctgaaat cgcttctgat attttttcca
    33781 tattcttttc cttcagctgc tgaccgttta aatagcggga aactgttccc actgaaacac
    33841 cagccatctt agccacgtca cggatcgatg ccatacattt tcactcctgc cagttacatt
    33901 tgatttcttt ttcttgttgt tcgaccatga tgaccgcttt ctttgcataa tgccgcttgt
    33961 catcaatatc catataacta aagtcttcag aagtgagttc aaaaacaagc agtctttcct
    34021 cttgagaccg caattcatct ttacgaaaag ctttcaataa tttcgttctt tggatgacaa
    34081 cgccgtcaat taatcggaca tagacaagag tacttgtttt acctgaaaca cttcctgtat
    34141 ttttaacttt aattgatatt attactttgt caatatccgt tttgacttcc atctgactgt
    34201 attcaaatgt tgtatagctt agaccatgtc caaaaggata aaatggactt ccgcttaaat
    34261 cataataatt ctcttgttta gaagcagcac gctgatagta ataaacaggt agttgccgac
    34321 tatttctagg ataagacaaa ctcaatcgtc cgcttgggtt atttcttcca gaaatcatct
    34381 gcgcaatggc ttttccaccc tcctgtccag gaaaccatgc tgttgcaatg gcattcgtaa
    34441 atgactcgat tttcatcaaa tcgtagggtc ttccttgcac aagtatcgtg atgattggct
    34501 tgcctagttg atacaattgt tcaatcagct gttcttggta tccacctaaa gaaagagaag
    34561 caacatctac attttctcca gaatccatgt tgatcccttt cgatgaaact gccccgttac
    34621 gaagaaattc catatcaaag ttccgcgcac ttgatccacc tagaactgca atgatcacat
    34681 ctgagttttg agcaagggtc accgcttcta ataatttttc ttcctgatcc tcctgatttc
    34741 ttacttcaca tccttgtgca taagaaacct ttgattgggg aaactctgtt tttatctctt
    34801 gtactatctt ttttttcaaa cggtcttctg tttgtggtgc agagtagtct cccaacaagt
    34861 ggtaaacatc gtgagcattt ggacctataa cagcaatgtt ttttacttgt tgatccaacg
    34921 gcaatatacc gtcatttttt agtaaagtga tactttcttt tgctgtctct aaatttaagt
    34981 gctctgattc ctccatgaga tgatcccaat actcctttgg atcgtttgca aatggctgtt
    35041 caaataaacc taataaaaat ttgacagata aaactcggcg aactgcttga tctagtagct
    35101 tttcatctac gacttgatta tcaatggctt ctgcaatctt ggtataggtc tcatcccaca
    35161 aactgagatc gattcctgcc gctaatgcat atgctgcagc agtcttttta tctgtaaaaa
    35221 catcggataa acgatccaaa gccacaccgt ctgccataac gattccttga taaccgatct
    35281 cttttcttaa tgtttgctca aaaagcgctc gattgatatg acaaggtatc ccgtcgatgt
    35341 cattataagc agccatgaca cctactgcat ttcttgtact cttcaataat ggtccgtaaa
    35401 tatcggagaa ctctctgcgt ccaatcgtta ctgtgcctga attatgaccg cctaaagctt
    35461 ctccttgtgc gatacagtgc tttaacacaa caccgatctg atccttcttc ttattgattt
    35521 cctctactgt ctgatcaaga aaagaaactt tttctgaaat aatctctcct tggaaccctc
    35581 gaacgaccgc ttcagaaaaa cgagctgaga gaatcggatc ttccccaaag cactcttctg
    35641 tccttcccca acgaggatct tttgccagat ccaaagtcga caccagcgct aaatgaacac
    35701 ctttagcagc cagttctttt gccatcaatc gactcgattg ttctaataat gctacattga
    35761 aagtatttcc acggccgata ttcgttggat aagaaatccc atccaagcct tgatgaccat
    35821 gtggacattc ttctacaagc aaaactggaa ttttccatcg cgaatgctgt agtacatatt
    35881 cttggactag gttagccacc ttccagcttt cttttgcagg aatcccattc ttataatctg
    35941 ttttagacca cggatcagag cgaaacagac cgtataaggc acccagaccg cctccccatt
    36001 taacatgttc ttttaacttt tctgttaatt cgatcttgtc tccgtttttt tcataacatt
    36061 cccaaccata caaatgctgg tttacctgtc ctactttttc agcaatattc atcctcgaaa
    36121 gtaaatcttc tactctttgg cggatggtta actttgggtt ttgatacggg taatcgtaca
    36181 ccttaatatc ctccttctgc caattttgcc ttattagcgg ctaatacaaa tggcaggaat
    36241 aaaaatacat ctagaatgat aagtattact gttaagactg ctgctcgtat atcgcctcct
    36301 gtagcaataa aagattgtat aaccggtgga gtgatccaag gtgcagcagc aactgtttta
    36361 ctgatgattc caagacttgt ggctgcgtag gcaataacca agttaataga tggtataata
    36421 gcgcatggaa tcatatatat tggattcatg actactggta atccaaacat aattggttcc
    36481 gtaatattaa ataaacaagt agtcagacca aatttagcga ttgatcgctg ttcttgacgt
    36541 ttagaagccc acaagatagc aataactaac ggtaaaatac acccgccacc tccgatcaat
    36601 ccaaatgcat tggtaaaggg ctccgtaata atattaggaa tttcttttcc tactgaaaaa
    36661 gcatccatgt tttcctgaat agactgtaac aaaggtgctt gccgtatagg agccaaaata
    36721 ctcgaaccat gcataccaaa cacccataag aaatcagaga aaaactgtaa aaataagatg
    36781 cctgggacgg ataaaacaaa agctttcaat ggtgtctgaa tcgctaattc aattaattgc
    36841 ggaacattca aaccagtcgt atggtttaaa gtaaaaacga caactgaaaa aaataaaatg
    36901 acaactattt ctggtactaa agaattaaat gacgcagcta ctgccggtgg aactccttct
    36961 ggcatcctga ttttaaaact cttcattttc gctaatttga taaataattc tgtactgaca
    37021 agggcagcaa tcatcgccgt tgctaagcca gacgaagaag tcagactttg taagaaaacc
    37081 cctgtagctt ctgctgtatt tccgtccatc aaagtgactg tacttgataa agacatcaaa
    37141 ataaacatta cggctgtact tagaattcct gcatgaacag gactaaatga tggatcatca
    37201 atcacgcctg tttggatata gtgggttgcc aaattccagc cgatattgta acacacgata
    37261 atagctaaaa tactcatcgt accattattc acgattgaag caagttcagc caatggactt
    37321 agatcaagta ttctttggac tgttgcattt gaaaaaatca gagcattcag caaaaccatg
    37381 acagaagcag ccattactaa cggcatagta acgataaaac tgtctcgaat ggccagcaag
    37441 tgtcgctggc tactcatctt agatacgatt ggtagcattt tttcagatag tctgtctacg
    37501 actttcatta tttcatctcc tagattctaa aattgaaccg gttcattttt aatatagcac
    37561 tttttttaaa gattgcaaca cattttttta aaacgctttc taaaggtggt ggatttttta
    37621 tgacttttga aaaattattc tatttaatgg tattttgtga attttttctc ttccatccgt
    37681 ttgaagtttt actttccaat ggtataattt ctttatccta tcaaatgggg gaagtatttt
    37741 gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga ctggctgtga
    37801 tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg tcttaaaagc
    37861 ggaaaaagca gatatcacca acagtgtata tacgctaaaa gtacgtaaag atatggattt
    37921 atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca cgtatcagac
    37981 acaaggagct gattcttctg aatcatctga agaaaaaaca gtgctgacag atatcaagat
    38041 tactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag aagcaacagt
    38101 cacatttacc aaaatcggta attaccgttt gaaggataag gatggcaaca tctacagtct
    38161 atagtgccat ctgttggatc ttcttttgtt tctccccttc ttttctgcta tagtaaagaa
    38221 gcaagatgac tgtaaaggag acgaaatgga tgatcgatag tcctgattat tgtacatgta
    38281 ataacaacaa aaatatccat acagaattta atgatcaagg aagtttgagt atatgcgaca
    38341 attgccacaa actagtcaaa gattctttta aatcgtttga atcttatcct gtcaaaaatt
    38401 atgtaaaaag caaaagaaga taagcaaaat caagcaaaac tcactatggc tgatttttat
    38461 gaacttcaaa ctcagcttct tcctttgatc aagcttcaaa aactggaatc agtaaaacaa
    38521 acagtggatc atttcagttc acaaatcaaa gagtaacgtt ttatttattt cacatctttg
    38581 aatataaaat aaccaaacga tagaaagact tattctacat cacgtagtaa tttttctttc
    38641 ccgtcgtttg gttttttact tttagcctaa ttcttgacct ttcgcatgct gattatctgt
    38701 aaaatatttt ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacgaat
    38761 cacctttttt gcatcttctg gattttctac aaaatcgaat gtatccccat ccacttgtat
    38821 tttaggacat acatcaaaat cctcatacca ttgattatag cgggtgttca gtgtttcgta
    38881 ataggtgtat aacgtttcat cgctttctag ctgttcatag tcacgcccgc gttttttgat
    38941 acgttctagc atcgtgtcca aagaaacttt gatatggacc atcaaatcag gacgtttttt
    39001 tggagcaaca tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac
    39061 ttcgatatcc gttacccgac ctaaatcagc atttagatgg aacaacagac tatcttcgta
    39121 gatggagcgg tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg
    39181 cttattcaaa aagaagattt gcaataaaaa tgcgtactgc tcaggatttg aataaaaaag
    39241 cggcaatact tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc
    39301 aagcatatcc gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga
    39361 catacaaccc ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatttttc
    39421 aaaatcatat tcacgtacag cttggtctga ttttaaatac gtgtgaagac tttgtgtaat
    39481 catcaagttc gtaggtaccg tctcttcttg tacgatgatc atcggttttt tcaacatagt
    39541 tgccacacct aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat
    39601 agccaaaaca aattctgcat ttgtcaactc ttccatatct ttttcgtata cttcctgcgc
    39661 ccatccagcg ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact
    39721 tgtgattgtt ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc
    39781 ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat
    39841 aactgatttt taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa
    39901 aagatcactt gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat
    39961 aattgttgca agccgctgta ataaccgcca atattcaata ttccgattgg cttatcaggt
    40021 gtcaggccga tcgctatcca agaaagaagt tgcgaaaatt cctccaatgt acctagacct
    40081 ccagggaaaa cgaggacagc atctcctttt tcaaataata atacctttct ttcatcaatt
    40141 gtctctgtag gaataaatgt agtaacatct ttcccaggca gctcagtttc aaaaaggcca
    40201 tttggataga tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc
    40261 ataatcccgg agtatgaacc accgtaaacc agttcgattt cttcttgtgc catatatttt
    40321 cctaacgttt gcgcaataaa tttgtaactt tctttattgc caaaacggga accgcaaaaa
    40381 acagtaagtt tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg
    40441 tttttttaaa ataaaagact agattttgta gttttttagt ggtatataga tcaaacacca
    40501 ctatatatag tatttgcaaa aaaataattt ttcagctatt ttacagtaac caaaaaaata
    40561 aataactagt aaatataaaa ttgaaacgac ttacttatag aagaaattgg gatttcgtcc
    40621 tgcatgtcaa taacacgcat tatttaaata gtcacacact tgtttctcct attaatgaaa
    40681 aatccatttt attctcgtgt tatgtttcac caacgcctga tgtttctcta aataaaaaag
    40741 ccgacgaacc aaataatcga aatttgatca ttttggttca tcagcttgtt cccaaacctt
    40801 tgtattaacg ataaatagct ggattcgtaa aactatcact tggatcatca ctgctggaag
    40861 aaaattcggt gataaccgcg cctgttgcac ctgcctggaa ccagtgttta atattttgtt
    40921 cgatcgtgta ctgatctcca gcgtgaagta tgatttcttt ttttgccgta tagtaacgtt
    40981 cttgcccctt tggaataata caggaaagtt tttttgtttc ttttccttcg acataaagat
    41041 atactaagcc ttttcgacaa cgaaaggttt ccttttttcc ttcattctca tctcgaggtg
    41101 ggtgccgatg ctctggacaa gtttgataag gcaataatac cagttctttc ccacagtatc
    41161 gctcattatt ttcataaaca aataaattca atccttcagt ctcgattgct cctaatccaa
    41221 aatctgtata ttcgattctt tctaattctg tttctttcaa tactactcct gaatctagga
    41281 acatttgttg tactcttttc ttgtaatcca ttctgttcct cctaactgat tgtcaagtca
    41341 tcccaagata tctcttcttc ctctttttct tcttggatga cttctgcatt tgggtcaact
    41401 ggttttttca ataataaaat agttattccc gtagcaaaag aaccagctaa caatgcacca
    41461 atgaatgccc atggtctggt catcgtagcg actgcaaaca ttcccgatgc cggaatacgg
    41521 ctatctgcgc cccaaaacat gctcaatcca ccgccgactg ccccaccgac tcccgttgct
    41581 atgacattgc gtgccaagtc gttcaatacg atagggatac atccctctgt gatctgacag
    41641 atccccatag gaaaagcggt cttcaatgta tcgatttctt gacgattgaa tatattttta
    41701 cgaatgattt tccctaagaa ataagcgatc gtcaagccga atggggttac catcgaagct
    41761 aaaatcaata cagtgattgg ttcatagatt ccttctgaaa atcgtgccac acccccttat
    41821 ccagaataaa tggtaatcca gaaacagccg ggtatctgcg gtaataagcc taaccaaacg
    41881 aaaaatatga gaagctaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
    41941 cagggcagct ttttcacaac ctcttttttt atttatgttt cataatcaat atggtaagga
    42001 tgcagctccg tatggtagac cgcctccgaa tattcaatca tttccccaat ttgcgtagac
    42061 gatttccgta tccgtttgat gccgatctgt gcgtctgttt tcagaatttc ttgttcttct
    42121 tctgttaaaa atactgccga gaaatcatca gaaaatgagt caatagtata tccttttttc
    42181 gtcagcaaac gatacaaaga ttctttgcca aattcttctc tgctgaattg catcatttct
    42241 tgtggaagat aatgaacaaa ataaatataa ggctgattgt tcaaataata taagcgctta
    42301 aaacaaacag cttgctcacc gaaaaaggaa aacaaaggat cttccttctt tatatggacc
    42361 aattgcaaat ccagcgtttc tttccggact tcatatcggg agttctccaa gatttgcgta
    42421 aagcttgccg ctttggataa tcgattgtaa ggacgattgc ttagaaccgt cgtcccacga
    42481 ccacttttct ttgcgacata ttcatctgat gccagcagtt cgattgcctt tcggacagtg
    42541 attttgctga cttgaaacag ttcttctaat tcactttctg tcggtaacat cgaaccgact
    42601 ggatattttc ctgagaaaat atcttcttta attttatctg cgacttccaa gtatagtaca
    42661 cgtgtccttt tcataacgtc accctttgct ttactattac taaaagaata gcacataaaa
    42721 aataaaaaaa gaacacaaat cacatgtgag aagtgtgttc tttttcattc agctgcttta
    42781 attacaagtt tacgccattt gaggcaatta cttctttata ccaatcaaaa gattttttct
    42841 ttgatcgatt cagtgtccca tttccttcat cgtctagatc cacatagata aagccgtaac
    42901 gtttactcat ttctcctgtt gatgcactga caagatccat acatccccaa ggcgtatagc
    42961 ccatcaaatc tacaccgtct gctactgcat tagccatcgc ttcgatatga cgacgtaaat
    43021 aatcgatccg ataatcatca ataatcgtgc catcctcttc catatgatca atggctccaa
    43081 gaccgttttc aacaatgaat aatggtttct ggtaacgatt gtataattca ttcaatgcga
    43141 ttctcagccc ttctggatcg atctgccagc cccattcact tgcctcaaga aacggatttt
    43201 ttactcctcc cagcaagttc ccttctgaag ttgctgcttg tggatcagtc tcattgattg
    43261 ccgtagacat ataataacta aatccgatgt agtccactgg atattctttt agcaactgga
    43321 agtctttttc cgtttgctct aatttttccg gatcgactcc atatttttta tgggtacgcg
    43381 ctgtgtatgc agggtattct ccacgtactt ggacatccgt gcagaaaaaa ttgaattctt
    43441 ggttttggat cattgttgct gcctgattga ctggattggc atcgatgctg taagttgttg
    43501 catagataat catgcagccg atttggatat ccgaacgcaa ttcgtggccg atttttacgg
    43561 ctttgctact tgcaacgaat tgattatgcc atgcttggaa aatattttgg tactctcctg
    43621 ctccattgct ttttactaat ccttgactta aagctggaaa gtgaaaggcc gaattgattt
    43681 cattgaaagt catccaatat ttgactttac tgctgtatct ctctaatact gtctgagcaa
    43741 atcgttcata gaagtcgatc agttttcgat ttttccagcc accgtattct tttgccaaat
    43801 gaagaggcat ttcgtaatga gaaatcgtga tcacaggttc aatatcatat tttaagcact
    43861 catcaatcag ctgatcataa aattctaatc ccgcttcgtt aggagtacct tcgtctccat
    43921 ttggaaaaat tcgtgtccag gcaatcgaaa aacgataaca tttgaatccc atttccgcaa
    43981 acaaggcaag atcttctttg aaacgatcgt aatgatcaat tcctcgatga ttcgggtaaa
    44041 tatatttttc ttgatcaatc gtccagtcaa attcttcact gccgataatc gcaaagcgct
    44101 gcttacctcc aggcatagca tctgcaacgg atagcccttt tccgtctcga tcataagccc
    44161 cttctaattg attggcagca gttgctccac cccataaaaa tttttctgaa aaacgtgatt
    44221 gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc aataatctct
    44281 ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac aactggagtg
    44341 attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa taaatctcct
    44401 gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt taattctact
    44461 gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat ccctacagca
    44521 tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag ttctcctact
    44581 tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg gtctggtact
    44641 tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg tgctgaagta
    44701 atcggttctc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca
    44761 aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt agctattact
    44821 gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat
    44881 aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat agctccacca
    44941 atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt cacaccatac
    45001 acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc taatgctcga
    45061 agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg tgccaagatg
    45121 gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt caaaaacatg
    45181 atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa acttcccatg
    45241 attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc gcctaaagca
    45301 ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac catcactact
    45361 aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt ttttacgaat
    45421 ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga agaagtataa
    45481 ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc cgcaccagca
    45541 actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa aaactggttg
    45601 gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt aaagacacca
    45661 tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca gcccatcacc
    45721 gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt
    45781 gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga ctcttctttt
    45841 ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac atctgctaca
    45901 ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat cacgtccggg
    45961 tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc
    46021 gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc ccatacacga
    46081 cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt aaaaaataaa
    46141 aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca atccaggttt
    46201 tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact tactactctt
    46261 tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt ttgcgtttgt
    46321 cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt tccttgaact
    46381 aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata acgctgggca
    46441 aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc gaacacgatg
    46501 ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc ggtcatcgtc
    46561 acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct
    46621 tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc ctctttcgct
    46681 gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt aggcagttgg
    46741 atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt cagataaata
    46801 ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg tttgacccat
    46861 tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt agcaaaagaa
    46921 tctcttaaaa caaattcttt ttggaccgct tctggcgaaa tctcgtcccc aatctttttt
    46981 tgaaaagcta gtccttttcc catacaaacg acttcttccc cgaatccatt tttcgaaatg
    47041 acgacattgt tattcaaaat tttatcaatt ttcattttgc ttcgccctct caagaaaaat
    47101 aaaaaaacct agaattcgcc tactgtttct tagctgaatt ctggttttgc ctgccgtaca
    47161 gtaacaatcc atttcttatt caaaatcatc ataccatcga atttttattt atgcaagcct
    47221 tttcatataa aaaataaaaa aactggaaca caagtctatc tagaaaacaa ccaaatcaga
    47281 gatgcacctc tttgcgtttc tctttttttc agcaaattgg tttttcctta tcgattcata
    47341 tcctttggct gtttttaatc tgtaacgact tatgtcccag tatcctaaaa tgtttttgtc
    47401 tctagattga aagcagatct catatcatct gtcgcatctt ttatcgatgt aacactttcc
    47461 agttctcctt gtacaactag tcgcgtgacg gcgtccatgt cgccacaagt aattagtgtg
    47521 acaatttctt ttccctcgac ttcattcagt aattgggtat ctgtaggagc aactttttct
    47581 ttaaaaaatg ttttgtatgt atacacattt tctaaatctg tcaaatagat cttggcaccc
    47641 atctctaaat tttctagcgg tgtaaacagc aattcagggt tcgttgcacg atggcttgct
    47701 aaggcataat ttccttttcc catttcttga tctggagata atgttcctgc tccgtaaagt
    47761 agtgacgtat tatccaagcc tttgaaaacc ggtaaattga ttgaaactga agggactgcg
    47821 atactagcaa tcactggtag atctgttcct ctcatttgcg acctcatgac cccttctgtc
    47881 gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt tatcgttatt ctttttcaga
    47941 tcttcttttg ttacattcgc tatagcatat ttatctcctg tctctctgac aaaataatcc
    48001 ttgatctgtt cattgaaaat caaagctagt cctactaata acaagaggaa caagaggata
    48061 ttgactagcc atctttttgc ttttcctttt tttttcattt tctctccctc cagactcatt
    48121 ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaca actctgtgta
    48181 tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt tgatcaattt
    48241 ttcttcaatg atctcatcta gatccaggtg aaggttgtct gccatcatgt atgaatagat
    48301 caatacatct gccagttctt ctttgattcg ttctaattgt gtttctttta cttcttccgg
    48361 cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag agatagccaa
    48421 atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt tgactttttc
    48481 catactattc atttgcttgc cttcttccaa tcacttctta cttgattaat cataccattt
    48541 ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt atttaaaata
    48601 aatgtcatag aaaatcaata atttaggtag ccaccatgtg tatgcagtag cgtgtcttct
    48661 tcccttgagg ctttttgttt ttcgatacgt tcttcttccc aaacagacaa cgctactaga
    48721 atctctctta atgattttcc ttcttcggtg agcgagtact ctactttagg cgggacttgg
    48781 ttataggctt ttcgatggat actttcatct tgttctaact ctcttaactg ctgggtcagc
    48841 attttttttg tgatcgtagg gatttgcttg cttctccacg cctaaaataa gcgatattgc
    48901 tcttcccttt atctggtgta agcaaagcag ttagcatttt gacgatcgtg ggttccgctt
    48961 ttcacttctt atctacttag tatcatgaag tatactttcg tactaatact aagtagcgga
    49021 agtgtcaact actttgttta actacatagc aaaagaaaca gaagggtgac aacggtcatc
    49081 acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct atctccgtgt agactttcct
    49141 caaacttatc ctttgacagt aagtcagtag gctgatttgc tctttctaga tgccagatac
    49201 atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc tgcattttcg
    49261 taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt tttagaggtt
    49321 tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt gttcgctata
    49381 ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact ctgatcggct
    49441 acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa aacaaccaga
    49501 tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat tttctccgat
    49561 tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac aatatttgtt
    49621 agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa tatttctaaa
    49681 aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa cagtagactt
    49741 ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat ttgatacagt
    49801 tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat aatggaaaaa
    49861 aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata attagatgag
    49921 cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa aatacaaata
    49981 cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct cgtctgtttg
    50041 tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa aaaacttgct
    50101 gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa aaagctgagt
    50161 ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt tttcgtagta
    50221 aaataaaaaa gaaacatcaa taataagcca attaggtaag gaaagctcac tttagggata
    50281 ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt tactagcagt
    50341 tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt caaataaagt
    50401 gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac acatattttt
    50461 ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag tttcacagcg
    50521 aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta
    50581 tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat gttttcttta
    50641 tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa atacgtaaaa
    50701 aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca gtagagatca
    50761 tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta tctgaacaaa
    50821 ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat gtcgataaaa
    50881 tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg
    50941 aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa gataaattag
    51001 tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac tttttacttt
    51061 taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat tgattctgct cattatgtgt
    51121 tagaattttt caacggtgac tccttactcg ttggtcgaaa gatctttgaa aagacaaagg
    51181 aacgtttcca tgacttccaa aaaactgctt cgagttgatg aataagagct gagacgtaag
    51241 catagcagat ttcagggagt gggccttgaa tcggctccac tccctgaagt cgttgatatg
    51301 attttacgct tcctcttctg taggagcatc tgttaacgac catgtgaatg ttgaaacgta
    51361 gttgtctaca gcataattgg atacaggaac aaataattcc actgattgtc tcccttgttc
    51421 cacattctct cctaatgaat aaatccatgt gccaatccct tcatttgctc ctgcagttac
    51481 tagatctact gcctgatctg ctgggatttc gattccttca gcgccagttc cagaaacggt
    51541 agatggcatt ccactgctat tagttgcgct atttcgaatc acggcgtttt tgaatttcac
    51601 ctttgaacca gttagtttat tcgtatcaga tgttgtacca ataaattctg ttcgtttgac
    51661 tgataatttc caaccggcat aatttccgct tttatccgtt acttgtacga aattctgtac
    51721 ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat gcagcatata ctgtttttgc
    51781 tgtacctaac ttgattgttc caaaatcaag atctggagct tggtcaatcg tcagtaatcc
    51841 ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta tcaggatcat ttggatcaac
    51901 aggaggaact ggaacaacgt cttcaggatt tgcagcatct ggtttggtca cagaaatcgt
    51961 tccttctgta gtggctgagc tcttattctc agcaaaaaca ctttgtgtac ataataatag
    52021 acttccagct agaacggtac ttaatgcaat tgtttttttc atttcttttt ccttctttct
    52081 tttaaaataa ataatccagc aaataagcca ataagaatga ctccaacaag tatcagccat
    52141 cgccagtcaa atgtttctgg ctgatttttt aaatcaatat ccttttcatt caattggtcc
    52201 gctgcatcat cagttatttc aaaatcggta tggagattcc agcgtttttc tttccaagca
    52261 cccttcacaa ccgcttgata atgtcccgga gctaattttt ttccttctaa tgggacaagt
    52321 aaatcaaaat ttgtattggg tgctacatct attttttctt tttttattga aaggtaaggc
    52381 tcttttgttt cttttttgta gatttcaacc gatgtagtca agccgaacat ataagaagca
    52441 tttggatttt gaagattcgc cttaatcaca tttcttccat cgctttgatc cgcataagct
    52501 ttttttaatt tcaaatctgg ggaagactct ttttcgctat ttcaaatcaa gattgctttc
    52561 acttgagaaa actgattgac gacaccaact gtacttttgt tagaaacgtt ctcttttttt
    52621 tcttgaaacg tgatccctcc tgcgaccaca ccagccagct ctttttctgg cgctttcagt
    52681 gaaagtgtct cagtgaccgc actttgagga ggaacagtta tttctcccac tgtttttgct
    52741 aagtttttca tatcataact taatgtttca tcaaatttac tttttgattc tccatattcg
    52801 atcacaccgt ttgaattcgt cgtagcggag tggatcaatg tctcaactgt tactgattta
    52861 tctgttcggt taaagaccgt gtaggggatt tcctgtgttt tcccaggctc catccttaca
    52921 tttatatatg ttttgttctt atcaatctga ttagccggta attcaaaatc taccgcaaaa
    52981 ttgaattcgg aggcatggac aggtgtctgc caaaggatga atccaaggag aaggatcata
    53041 agtaaagagc agttgtttcg atgactttta ttccatattc gattcatcaa tcatcctcct
    53101 tgatctttat tctgtcggcg catctgctaa tgtctatgtt atctctgctg agtaattacc
    53161 agctatctga ttttctttgg ttaagacaag tttaaacgcc cgaccgttat ttaaatattc
    53221 tgaataatct gcttgcacat tcgttgaatt ttgtcctctg acaatttcca gttcttcttc
    53281 attcactaat tgatactcct ctcctttctt gaaatatagg acatcctgca tagaaaatcc
    53341 tgcatcattt gtgaaaggct gagataattt tatcgttaag atccacggat ttttcgtttc
    53401 tcgctgatca taaatccata atttttcctg tatatccatt tggccaaatg cttcttgtgt
    53461 caaaccagtt gctttgatct gaccaaaatc gagtttggaa ggggcaagaa atgagagacc
    53521 accttctact acgatcttcg tagcaggtgc aaaaggcggg gcatcatgat aaacaactgg
    53581 cgtatcactt ggattgtgat tcccaatttc atcgttggta cttggcttgt tctgcacacc
    53641 cttagcagta gcttctcctg cttggtcgtt taatacaatt tctaatacat ctcctgcaaa
    53701 aagttgattt tctgggatag aaagaacaaa ttcccctgta tcatcaatag ttgtttctga
    53761 caaaacagct tgattttttt tcaatgaaac caaggctcct gcttcacctg tacctgtgat
    53821 cgtgcgagaa gctgcattga tcaatgtggt acttaaatta gcaggttcag gaggaatcac
    53881 atcgactgtc gttttggcag agtccagttc tgtcacttct tggccacctc ttactttttt
    53941 agcagcaaca acttgcactt gatcctccgt ttttaatctc tcatcattgg gtacttgaac
    54001 ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta tatcctgccc cttcattttc
    54061 atctgtttgt ccttgcgtga gagcagtaat ttctacagat gatttcgtcg tatctgcgta
    54121 agtaatacgt aaagtcactt ctacttcgcc tgtccaagca tctcttggct ttgcctcgac
    54181 accttcggga acaacgacat gcccatagat ttttttatct gcattcgttg gcactcttaa
    54241 ggattcaatc atcgggagct gattattggc actgattcga ttataatttt tcattccggc
    54301 acctgtctga gcaaattcat ttgttaaacc tgcactaggg cttgatacga aattgcctaa
    54361 gtcggtccca ttgatcgata agtctgtcat atcagaaaaa taatccgggt ctttttcaaa
    54421 gttatcgaat gttgcgcctg tttttcgcca taaagcgatt tctgaatggg cgatctttaa
    54481 agaagaatct ccatttcctt ggaataccca gccgcctatg ccattggtct cgcttccaat
    54541 ccgtttgttt tggaaatcaa aataggtagg agattctaag tccacattca acacatcccc
    54601 actgatgatg ccggtcttat cttcccctgt tttcccgacc atgactaaat aagttttttc
    54661 acctgccgta aaatttaaat caatatctcc agttgtatct acagttgctc cggaatctgc
    54721 gcgtatatcg atatttgatt tttctccagt gactgtaaaa tcagctgaac ctggactttc
    54781 tagaaaacca gaagcgtcat aaaattggat cgcttgattt ccttgattga gtctgttcac
    54841 gtaagatttg ccatcacctg tatttcttac cctcaatgag cctccattgg ttactgtcag
    54901 cttttgcgta ccattctcaa aacgaactgc gctggaacgc ccactctgct tatcgatgtc
    54961 gataactgat ccggctcctt ctacgatcaa agaaaggcct tttcctacaa aacgcatccc
    55021 attgttcccg ggttgatcgt catctccagc cattttgacc gagatttgtc caccacttga
    55081 tacatggatt tgactagaat ctccttgcaa gcgaaatccg cccgtagttg ctgtattgat
    55141 ggtgattttc ccaccattcg atacagtgac tttcgatcct gaacctctca tttggatgat
    55201 tccgcgattg ttgtcattgg taatattgat ctttgtattt accgaaaatt cgcttcccga
    55261 accactgact tcaaacatga cattaccagg tagcgtatcg actgcctctc cttcatcgct
    55321 aacgccatct gcccgatcat aatcaagaga gacctttgaa ccgcttaaga tatggattcc
    55381 tggcgctgtt tttccactac cagtctcatc tcctgaatct agaaaaccgt agaatttccc
    55441 tagatacggt ccataaagtt gactaccatt gatcacattc aattgatagc ttctacaaaa
    55501 ataggcttta cccggagcgg cattcagtcc ttccttcact ggttggacat ccaacacacc
    55561 ggaaacacca tctaatgtga cagttgcacg aggagcgtaa atcagtccta atttggaccc
    55621 atcatacaaa tcaagtgtcc cggtaaattt tatttctccc gtaaaatgat atctgcttcc
    55681 ccaaaaatcg gtcatataag caatgataat ggcatttcca tcgatatttg gtgtcaatcc
    55741 acctggatgt gcagctgttc ctttaaaatt ctggaactcg atcgactgag gtatctcatt
    55801 gatataaata tggtgtgtgc cgaaattgtg agcgaatcca ccaaaatcaa taacgatatc
    55861 tcgtttgatc tctaattccc tgtctaaagt caaaggctga atcagtcgga tcttagttac
    55921 tgattcgtct gcgagaagct gcgccagttc ttctgttgtg gcagcttcgc tgtacccatc
    55981 cgctgaaaca gctcttgcca gtgccgcttc actgctatca gacgtttgtt cagctacttc
    56041 tgaggtctca cttgtcttat tttcttcttg ccttttatta gtagaagcct cactttgaga
    56101 ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta gtcttgtcaa aactttgatt
    56161 ctctgattgc gttgattcgt ttgttttctc tacatcggca aaaacggatg tcaccatcaa
    56221 gagagggaag acaaacaaaa tcgacgcaat cagctttttt acacattgat tcattctcca
    56281 ccatcctctc tgacgttttg acaattttat tttatcgttt taatctattt ccaattgtac
    56341 tttcatattt aaaatatgta tttttatgga aaaatttttc tattaggtgt aaatttcttt
    56401 tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag tcccttatcc cgaacaagcg
    56461 gtgttctccc gccaactcct cggtcacaac ctccatttta acaataaacc tgtaaatgtg
    56521 agggcaatac tttgattgtc acaggtaatt tatccccttc atctccatca acattcgtcg
    56581 tcaattcttc ctcatcagct aatgacacaa ctaattgctc acaagtaagg taggctagat
    56641 ttttcgtaga ttcatctact ccttttaata ggtctggcaa tgttttaagc gtatcgatca
    56701 aagaagaatc ttttaaataa accaaatgga gcttaccatc atccactttt gcatttggaa
    56761 gtaatgtttc aaaaccgccg actgagttag ttagtcctat cagcaaggta ctgctctcaa
    56821 tttcttcctt ctttccatca acttctacgt gaaacgagta ggattgtgtg gaagctaact
    56881 gtttgattcc tgacatgaaa taagctagtt ttccaaattt cgtcttcttc tccgcatcta
    56941 catcattgat tgattcaggg atagagccaa ttgcgacgac attcataaaa tagtcgtcat
    57001 ttatttttcc aatatccaat ggcttgactg attcaatcga aaaatgattg atcgcttcct
    57061 caggttctag cggtatccct aatgctcgag ccaagtcatt caccgtaccc aatgggaaaa
    57121 atccgaaatt tggccgatgt tcttgttcag cgattccact gattccttcg ttgactgttc
    57181 catctccgcc catgacaaac acactatgat acccttccgt agccgcttcg cgagtaaagt
    57241 tttttgcatc ccctgctttt tttgtatgca agacgaccac ttcatcaaac accgattcca
    57301 attttgctat cgctaattgc tcaaattcct ttgcttgttc accgcctgaa ctggggttta
    57361 ccactaataa aacttttttc atcactttct ttcccttctg attgatttct ttaatataac
    57421 gatagcataa agaaaaaaat taggctgtga tgaagtactg ttcatgaaac attcatttat
    57481 tttttgacaa gataaaacat ccatactatg aaactgagga aaattaaaac aatgatatag
    57541 gaaataaaag aaaatgatag gcattgtata cctaataaag taaaaatctt aggtggcaga
    57601 taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct tccacttttt gttatcagac
    57661 cttatagcga tcagaggaat accaaatgca taactgaaaa actcttgatg tactgttaat
    57721 atgtcacggc cttgaataat aaatgcgaca atccccgcta taattagtgt gcctaacacc
    57781 aaaacggaaa attgatgaat atattttata tacaaatgat ccattgtact tcctgccttt
    57841 ttatttgtga gaaaaacaaa tccgtatacg tgaataactt ctagaaataa aagaaatttg
    57901 caaaaccact ccatttatct acttctacgt gcattttttg atattgtttt ctttatttaa
    57961 gattatacag aaaaatatct agggtcgctg gattctaagt aaaaaaaatt tttaaaataa
    58021 aaagaactaa atcaagtagt aattaaacta ctgttgattt agttctaata aaaagattta
    58081 gatgaacagt tttattgatt taaatagttc atatttttta tttataccgc ttatttacta
    58141 cttgctgtac tagaatctga agtgaatgta ctcaaaacat tttcaaagtc tttgtctttg
    58201 atttttacat tagcgtcttt caatacttcg ccaattactt tattctggaa ggtactgtca
    58261 cttaattttg tgtctgttgc aatttctttc aattcttttt tgtatttatc catgtcgttg
    58321 cctttgtttt ggtttttcac cattttaacc acataatatt ccgtagtata agttgaagca
    58381 ttggtagaag tgatcacatc agaaacttga ccatctttca acttaaaggc tgcttctttg
    58441 acttctgccg gaaccgttgt agtagtggaa tcaaatttca cttttccgcc atcttctttt
    58501 gtcgtagtat cagttgactt gtctttggct aatttagaga aatcgtctcc cttatcagca
    58561 gctttcttca catcttttgc atcatcttca cttgctactt ggataatttg agcttccact
    58621 tctggatgga aagattccca agccgttttt aaatcttcat cggtcaattt gatatgtttc
    58681 tttagacctt cttgaaaggc tagactttgt ttgatactgt ctttgaatgt ttgttctgtg
    58741 taacctgcag atttcaattg tgaatcaaat gaatcaccca attgtttctt tgtttgatca
    58801 tattgagaat cgatttcttt ggttgatacg tcatctccat atttatcttc aaatacttga
    58861 taaacgatca actgttgcac agcggaatct gctccttgca tttttttcaa gctatccatt
    58921 acttcttgtt cgctgattgt accgccttta taactaacgg ctgttttttc accggcattc
    58981 gtacagcctg acaataagcc agtgcctagt acgatggcaa gtgctaaaaa tctttttttc
    59041 atttatcaat tctcctactt ttcgtttttt gcgaattgct aaaaacaaag gtgttcaaga
    59101 tgaaaatcat caagaacacc tttactaatc tgctcgatac gtcctgaata gatataaggc
    59161 tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa gtagcacatc acaccttaat
    59221 atactacttc ttttgttacc gtttttcaag tctatcttgc gttccgtatt taacgtaact
    59281 aattcttaga acaaatgtta tcgattttgt ttttcttctt ccgctgctgg gatattcaaa
    59341 tcagacactt ttatgttcga ttttccagac atttttttgt tgtttttatt atgatatatt
    59401 tttatacaac taaaaatgaa tatattaaaa ctaccaaaaa taacttttaa cgctgtttca
    59461 ttatatgttt tttctatttt atataaactt ttggatactt aataccaacc ggttggattg
    59521 taccaacatg agaatgtgtc agttctattt cctgtcgcac ttgcataggc ccatactcct
    59581 tttgttgcat ttgctgatcg aatagagttt ccaactttcg cttttgcacc gtgacgatta
    59641 tagacacgac tatagtggtt agaatgacct gtttttctcg tacaagttgt atcactataa
    59701 taaacccatc ttccagagct atgattaatt gctgttctag aaatttcccg gtctactggt
    59761 aaatttgact taataatatt acctttagca tcaaaatgaa tctctcctgg ctttaaagat
    59821 ttgtaataga tttcaccgtg aactgaattt gcttccactt cagatgacat agcgtttcct
    59881 ccaacaaaac ctatccctag tgcacaaata ataactgctt ttttcataaa acgacatcct
    59941 ttcctttttg atttttaaag catgatcttc ttgtttccta ttttggtcag atatggatca
    60001 tgcgtgacta cgataactaa cgtaccttgg gaagcttgtt gcgataagat ttcaactact
    60061 ttatccttat ttcttttatc caatgaccca gtcggttcat cggctaaaat gattttgcta
    60121 ttcttcaata agactctcgc acaggcgatt ctttgcttct caccaccaga taatgtgtag
    60181 ataggcgtac ttagcttctt gtttatattt acttttttca atgcctcata ctttgctgtt
    60241 cgcttatctg aaacatttaa attcgaaaac ttcaatccaa tatctaaatt aaaatcgatt
    60301 gtttcatatt caatcaaccc aaaatcttga aaaagaaaag atatggtatt tctttttatc
    60361 aagcgcttct ctctcccagt taattgtttc gtctcttgat cgaacaaata gaattgtcct
    60421 gaactgaact gatcattcaa tcctaatata ttcaataacg tacttttacc tgctccactt
    60481 tctccaacaa cagtcacaag atccccagca tccgcagtga aacgatccaa atgaagaatc
    60541 gttttgtttc gaatgatttt tttcgcacct tttatcacta tcaacggctc caattgttaa
    60601 cctccattca tcagcaataa ctgacttttc ttttctgtag acaaaataaa aacaactgaa
    60661 agaataaaat caatgaagct aaagaaaaaa atattaaaaa gatatttagg ctctctttct
    60721 ataagaaaaa ggaagattac acttgtcgtt ccccatttga tgaggttcat catgaagaaa
    60781 tctttatacg tttcaaagag cgaataacca cttgaacgaa agatagcgat tttccgacaa
    60841 ttgtagacaa aatatatgca taacatgaca tagctcaaca agaagaaaga aaagaaagtg
    60901 agtaaaaata tctgtatcaa gtaatgaaat gatctgctta acactttgac agctatctct
    60961 tcagtctgag tatacggaaa tgataactgc aagttatcac gtaactgact ttcttcaatc
    61021 agctcaaata acctttcttt cgacaaagag cctatcttga tctttaacgg tggatagatt
    61081 tctccgctga gaatatttct ttccaaatac atactgtttt tcaatgtcag tatttcaaca
    61141 accggataat gttcgatcca ggggatagaa ggaataaatg tataaatggg ttgattgttt
    61201 tttataaata gaaatttcgg ttctttttca ctgatttctt gataatattc tgttatttgc
    61261 gccaggtcat tttttaaatc tgtatgtcta tatcgttcag ggatcaatat aacagggtgc
    61321 gtttcttttt cggttatttg gatcaacctt ccagagacat caatcaaagg aaatttctta
    61381 agataattca gatttatttt tatccctctg ccaaactgtt cattttcttc attcaagtaa
    61441 tctgatacat tgactaatat acttccgttt ttattcaaag tttcatataa ttcctgatct
    61501 gctgctgatg caaaacgatt ggaataaata aaatccacgt ggtttttccc gatggttact
    61561 ggataaaata catgatagtc ctcaccagcg atttcttttg gtggttcatg ggaaacagct
    61621 aactgtgaag aagaaaaaac gatagttgct aattcgacat tggcaacggt caaaagaaga
    61681 gaaaacaaca atccaaatgt aaaaagaatg tattggatat agttgaacgc ttctcttaac
    61741 gaagcgattt tttcaactaa taaaagagaa atcaaagaga taccatatat tccgctgaac
    61801 aagaaaacgt acatctggaa caaataaaga aattgctcct tataatacat aaaaataaaa
    61861 ccaccaatga ttcctgcaat tatgaatgta ataatgattt tctttttccc caataaacac
    61921 cacaatactt gatgtaatga aaagtcatta ttcaacaata aactgatttc tttattcatc
    61981 aataagaaaa aggatgataa caaaaggaat aaaagaatca atccaatagt taaatacact
    62041 cgataattat atacgtctgt atttgaaata accgaatacg aatagctatt ttcaaagtct
    62101 tcaaaaatat tagtggcata atcagtgtga aatgcttgat tgaatcgatc agtgaatagt
    62161 gaaatgaccc gttcataggc ttctttttct ctcgtcatca agaaaaattc accttcaaaa
    62221 tcttttattt tgatcgattc ttcaatggac gttacgcgaa aggaggtatt agaatagggt
    62281 accttctcgt gagaagattt gacatcccct tgataataca atgtcatttc aagctttggc
    62341 tgtatcttga agttccaaga tgtatctact tcattggatt catttactgt ttttttcata
    62401 taagaaagct cagtttctga tgagacattt tcaaatacca tgattagctg ctcatattct
    62461 tcttgcccaa taaaatgttg tggcaatcga atcggagtat aagtatctgt ttgatggaaa
    62521 aattcatttg tccgatagag tacctgttta tttatactaa ccaaggcaaa accactcatg
    62581 ataccggtca ctatgattgt gagtaaaact gtgaactttt ttttgattat caccactctc
    62641 ccttacttaa atgattcttt gataaaatca taccgttttt catcaaaaaa acgtatctat
    62701 caagtgtata ggtcatgtag aataaatcta tatactgtag aatcgaaagg agaagaagat
    62761 gaacaccgga gaaaaaatac gaacttaccg cagaaattgt aagcttacgc aaaaagaatt
    62821 aggcgaaaaa attggtgttt ccgataaaac gatttctagt tgggaaaatt ctcgtacaat
    62881 gccagacttt gaaatgctgt cacttttaca tcatacttta ggtctgccga ttgattttcc
    62941 ttctattgaa tcagctaccg aatcaataga accaacattg ccagatgaac ctcttgttaa
    63001 caagaaatct tattcatcaa aacaaccctt ctcactcaaa aagacactat ctttctttct
    63061 gctcgtcttt tgtttggtct atctttttct tatcaattat caatacaaaa atgaaacgat
    63121 cgatcgattc aatccttttt tgaaaccaca aatcggctat accacactcc ctgtaggata
    63181 cgtggacaat tccatcgaac gttgggtatg taatgatatc tttggccatg gtacatggct
    63241 gtcttttcac gctggagagg tagaaaaaga gaaaaaccat gctgtaatca aacacaaagg
    63301 aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc attccctcag attaccaaga
    63361 aatcctcagc caagactatg acgacgcctt aaaaagaagc cctcaggagc cagataggca
    63421 ttaagtagga gatgaacgta gagatagaaa aaatcaatgc acatgatctt atccaaaaaa
    63481 caggggtaca aattaattga aaaagccttg agatgaaagt tcctaaataa aaaagttcag
    63541 aatgataggt atttctggct tatagtataa tatttacgcc acagccttcc aatgattctt
    63601 tttggaatca taatcaagga tggacattca caaaaatatt ttgctcattc gatccgaata
    63661 gattaacgac caatctagta agtaatatag attggtacgt agtcaagctg cacataattt
    63721 agatcaattt tagttctttt ttatatttct atgatctgct catttacttt tatatgatgg
    63781 cttacagtaa cataaataaa tatgataatc gctggtagtt tatccaagag tactcaataa
    63841 aatacaaaat ggattgttca agcgcctatc ataatgataa aatgaaacaa ataaacgata
    63901 caaggaggaa gagactttga gtaatggcag aacaattttt tcattgtaaa aattgttcag
    63961 ttgttaatct atgggatttc ggtagcatgg gcattttttt atagaggaat cacaccaacc
    64021 tggccgtttt atctcttgtt agtgggaata ttagtaggaa attttgcccc tcttcactat
    64081 cgtacgaact tttttccaac tgattctggc ctttttctta aaacaaagac gcgaaaatta
    64141 gaaaacatta ttgaaatgac aattacctgt ataatcattt tcttagttat attcctaaac
    64201 gtcttctaat aagaatctct taaaattagc ttgttacagt tagttaccta gtttatataa
    64261 cacagttatc ttatcttcct tatcaacatc ttttgccaac tctttgtctt aaattcgaaa
    64321 catataagca gagctacgaa caccaagact gtttcggctc tgttttttgc atcaaattca
    64381 ctcttatata gctgataatc tcggctcttt aaaagaaatt attttcgctt cattttatta
    64441 taaaaaggct aggaaccttg atgttatcag gttcctagcc tttatttaag tattacccca
    64501 cagacccttc catatagatt ttcaaattac acactagttt aagataaaac aaacaaggaa
    64561 aaacttacca tttactcaaa aaattaactc atataagtac attagaatgt aaaatagact
    64621 ggtcaaaatc tggtcaaaaa agtagatgtg acacaagttc gcattcaaag cgatgatcca
    64681 tgatttcgta atggtcaatt atatggatca aagccacgcg acaagagcgc aatggatgtc
    64741 tctaccgatt ggttgaataa acgaaataaa tagaatgtga aaaaggatcg gacaaatgtt
    64801 ccagtttgct cgatcctttt ttatttgaat tcattttcgc tttatcaaac atcatttgat
    64861 tactcagtta aatacttttg acatgagtac caatgaaaca tttattcatt aaaattactt
    64921 atttcttatc gcctttctag ccaatgccaa tccgccgatt attccagcac tatcacctaa
    64981 ttcgggcgta acaatgtagc tagcaattgg tggtatttca acatatccat ttataagctc
    65041 aacaaattta tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa
    65101 aataattcta tctggcgaaa gcatcagtgt tgtattgtat gcacattggg caaggtaaaa
    65161 ggcttcaatc tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaatcg
    65221 tttttcaatc gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt
    65281 tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact
    65341 gaacccttcc acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt
    65401 gaagtatgca acacctgcta accctttgcc agaaccagca acatattcac catagcaagc
    65461 cgcattaaca tctgttgtcc attcaatcgg aatattgaaa tgttgcttta gcgttcctac
    65521 aaaattgaaa ttctgccaag caagtttcgg tgttgatgtg atatgaccgt atttctttga
    65581 tttacgttga atatcaattg gaccaaaaca gccgaccccg attcctgcca attgttcctt
    65641 aaaaggttta aaaaaatcaa tgacctgctt cattgtttca actggagtcg ttgtgggaaa
    65701 actgactctt tccaaaatcg tcaattcctc agtactgact gcacaaataa atttagtccc
    65761 tccagcttca attgccccat atatcatcat aggcttctcc cttcgaatat gtgtaaaaca
    65821 cttcattaac gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct
    65881 tctgttgaat accaata
//
LOCUS       DACIFS010000007        60891 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.007, whole genome shotgun sequence.
ACCESSION   DACIFS010000007 DACIFS010000000
VERSION     DACIFS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.007"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(9..590)
                     /locus_tag="IYA83_000441"
     CDS             complement(9..590)
                     /locus_tag="IYA83_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8585904.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(723..1406)
                     /gene="rpiA"
                     /locus_tag="IYA83_000442"
     CDS             complement(723..1406)
                     /gene="rpiA"
                     /locus_tag="IYA83_000442"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ8585905.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1725..2138
                     /gene="rpsL"
                     /locus_tag="IYA83_000443"
     CDS             1725..2138
                     /gene="rpsL"
                     /locus_tag="IYA83_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ8585906.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2222..2692
                     /gene="rpsG"
                     /locus_tag="IYA83_000444"
     CDS             2222..2692
                     /gene="rpsG"
                     /locus_tag="IYA83_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ8585907.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2786..4873
                     /gene="fusA"
                     /locus_tag="IYA83_000445"
     CDS             2786..4873
                     /gene="fusA"
                     /locus_tag="IYA83_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ8585908.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5045..6232
                     /gene="tuf"
                     /locus_tag="IYA83_000446"
     CDS             5045..6232
                     /gene="tuf"
                     /locus_tag="IYA83_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ8585909.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6639..6947
                     /gene="rpsJ"
                     /locus_tag="IYA83_000447"
     CDS             6639..6947
                     /gene="rpsJ"
                     /locus_tag="IYA83_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ8585910.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6978..7607
                     /gene="rplC"
                     /locus_tag="IYA83_000448"
     CDS             6978..7607
                     /gene="rplC"
                     /locus_tag="IYA83_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ8585911.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7635..8258
                     /gene="rplD"
                     /locus_tag="IYA83_000449"
     CDS             7635..8258
                     /gene="rplD"
                     /locus_tag="IYA83_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ8585912.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8258..8548
                     /gene="rplW"
                     /locus_tag="IYA83_000450"
     CDS             8258..8548
                     /gene="rplW"
                     /locus_tag="IYA83_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ8585913.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8587..9420
                     /gene="rplB"
                     /locus_tag="IYA83_000451"
     CDS             8587..9420
                     /gene="rplB"
                     /locus_tag="IYA83_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ8585914.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9463..9741
                     /gene="rpsS"
                     /locus_tag="IYA83_000452"
     CDS             9463..9741
                     /gene="rpsS"
                     /locus_tag="IYA83_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ8585915.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9764..10111
                     /gene="rplV"
                     /locus_tag="IYA83_000453"
     CDS             9764..10111
                     /gene="rplV"
                     /locus_tag="IYA83_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ8585916.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10125..10781
                     /gene="rpsC"
                     /locus_tag="IYA83_000454"
     CDS             10125..10781
                     /gene="rpsC"
                     /locus_tag="IYA83_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ8585917.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10784..11218
                     /gene="rplP"
                     /locus_tag="IYA83_000455"
     CDS             10784..11218
                     /gene="rplP"
                     /locus_tag="IYA83_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ8585918.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11208..11396
                     /gene="rpmC"
                     /locus_tag="IYA83_000456"
     CDS             11208..11396
                     /gene="rpmC"
                     /locus_tag="IYA83_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ8585919.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11421..11687
                     /gene="rpsQ"
                     /locus_tag="IYA83_000457"
     CDS             11421..11687
                     /gene="rpsQ"
                     /locus_tag="IYA83_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ8585920.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11745..12113
                     /gene="rplN"
                     /locus_tag="IYA83_000458"
     CDS             11745..12113
                     /gene="rplN"
                     /locus_tag="IYA83_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ8585921.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12150..12458
                     /gene="rplX"
                     /locus_tag="IYA83_000459"
     CDS             12150..12458
                     /gene="rplX"
                     /locus_tag="IYA83_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ8585922.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12486..13025
                     /gene="rplE"
                     /locus_tag="IYA83_000460"
     CDS             12486..13025
                     /gene="rplE"
                     /locus_tag="IYA83_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ8585923.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13044..13229
                     /locus_tag="IYA83_000461"
     CDS             13044..13229
                     /locus_tag="IYA83_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ8585924.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13265..13663
                     /gene="rpsH"
                     /locus_tag="IYA83_000462"
     CDS             13265..13663
                     /gene="rpsH"
                     /locus_tag="IYA83_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ8585925.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13695..14231
                     /gene="rplF"
                     /locus_tag="IYA83_000463"
     CDS             13695..14231
                     /gene="rplF"
                     /locus_tag="IYA83_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ8585926.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14392..14748
                     /gene="rplR"
                     /locus_tag="IYA83_000464"
     CDS             14392..14748
                     /gene="rplR"
                     /locus_tag="IYA83_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ8585927.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14769..15269
                     /gene="rpsE"
                     /locus_tag="IYA83_000465"
     CDS             14769..15269
                     /gene="rpsE"
                     /locus_tag="IYA83_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ8585928.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15284..15463
                     /gene="rpmD"
                     /locus_tag="IYA83_000466"
     CDS             15284..15463
                     /gene="rpmD"
                     /locus_tag="IYA83_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ8585929.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15507..15947
                     /gene="rplO"
                     /locus_tag="IYA83_000467"
     CDS             15507..15947
                     /gene="rplO"
                     /locus_tag="IYA83_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ8585930.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15947..17242
                     /gene="secY"
                     /locus_tag="IYA83_000468"
     CDS             15947..17242
                     /gene="secY"
                     /locus_tag="IYA83_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ8585931.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17304..17951
                     /locus_tag="IYA83_000469"
     CDS             17304..17951
                     /locus_tag="IYA83_000469"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ8585932.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18142..18360
                     /gene="infA"
                     /locus_tag="IYA83_000470"
     CDS             18142..18360
                     /gene="infA"
                     /locus_tag="IYA83_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ8585933.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18393..18509
                     /gene="rpmJ"
                     /locus_tag="IYA83_000471"
     CDS             18393..18509
                     /gene="rpmJ"
                     /locus_tag="IYA83_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ8585934.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18527..18892
                     /gene="rpsM"
                     /locus_tag="IYA83_000472"
     CDS             18527..18892
                     /gene="rpsM"
                     /locus_tag="IYA83_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ8585935.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18920..19309
                     /gene="rpsK"
                     /locus_tag="IYA83_000473"
     CDS             18920..19309
                     /gene="rpsK"
                     /locus_tag="IYA83_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ8585936.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19389..20327
                     /locus_tag="IYA83_000474"
     CDS             19389..20327
                     /locus_tag="IYA83_000474"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ8585937.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20355..20735
                     /gene="rplQ"
                     /locus_tag="IYA83_000475"
     CDS             20355..20735
                     /gene="rplQ"
                     /locus_tag="IYA83_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ8585938.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21007..21846
                     /locus_tag="IYA83_000476"
     CDS             21007..21846
                     /locus_tag="IYA83_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ8585939.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21822..22691
                     /locus_tag="IYA83_000477"
     CDS             21822..22691
                     /locus_tag="IYA83_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ8585940.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22681..23478
                     /locus_tag="IYA83_000478"
     CDS             22681..23478
                     /locus_tag="IYA83_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ8585941.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23681..24427
                     /gene="truA"
                     /locus_tag="IYA83_000479"
     CDS             23681..24427
                     /gene="truA"
                     /locus_tag="IYA83_000479"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ8585942.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24760..27429)
                     /locus_tag="IYA83_000480"
     CDS             complement(24760..27429)
                     /locus_tag="IYA83_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ8585943.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27667..27855)
                     /locus_tag="IYA83_000481"
     CDS             complement(27667..27855)
                     /locus_tag="IYA83_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ8585944.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28125..28487
                     /locus_tag="IYA83_000482"
     CDS             28125..28487
                     /locus_tag="IYA83_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ8585945.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28539..30221
                     /locus_tag="IYA83_000483"
     CDS             28539..30221
                     /locus_tag="IYA83_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ8585946.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30338..32374
                     /gene="recG"
                     /locus_tag="IYA83_000484"
     CDS             30338..32374
                     /gene="recG"
                     /locus_tag="IYA83_000484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ8585947.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32423..33424
                     /gene="plsX"
                     /locus_tag="IYA83_000485"
     CDS             32423..33424
                     /gene="plsX"
                     /locus_tag="IYA83_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ8585948.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33546..33788
                     /gene="acpP"
                     /locus_tag="IYA83_000486"
     CDS             33546..33788
                     /gene="acpP"
                     /locus_tag="IYA83_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ8585949.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34056..35060
                     /locus_tag="IYA83_000487"
     CDS             34056..35060
                     /locus_tag="IYA83_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585950.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35061..36005
                     /locus_tag="IYA83_000488"
     CDS             35061..36005
                     /locus_tag="IYA83_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585951.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36005..36967
                     /locus_tag="IYA83_000489"
     CDS             36005..36967
                     /locus_tag="IYA83_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585952.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            36994..37908
                     /locus_tag="IYA83_000490"
     CDS             36994..37908
                     /locus_tag="IYA83_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8585953.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            37934..39715
                     /locus_tag="IYA83_000491"
     CDS             37934..39715
                     /locus_tag="IYA83_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8585954.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40063..40749
                     /locus_tag="IYA83_000492"
     CDS             40063..40749
                     /locus_tag="IYA83_000492"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ8585955.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            40769..44350
                     /gene="smc"
                     /locus_tag="IYA83_000493"
     CDS             40769..44350
                     /gene="smc"
                     /locus_tag="IYA83_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ8585956.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            44359..45168
                     /locus_tag="IYA83_000494"
     CDS             44359..45168
                     /locus_tag="IYA83_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8585957.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            45179..46177
                     /gene="ftsY"
                     /locus_tag="IYA83_000495"
     CDS             45179..46177
                     /gene="ftsY"
                     /locus_tag="IYA83_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ8585958.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            46364..48631
                     /gene="gshAB"
                     /locus_tag="IYA83_000496"
     CDS             46364..48631
                     /gene="gshAB"
                     /locus_tag="IYA83_000496"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ8585959.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(48720..49172)
                     /locus_tag="IYA83_000497"
     CDS             complement(48720..49172)
                     /locus_tag="IYA83_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ8585960.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            49322..50272
                     /locus_tag="IYA83_000498"
     CDS             49322..50272
                     /locus_tag="IYA83_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ8585961.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            50265..51224
                     /locus_tag="IYA83_000499"
     CDS             50265..51224
                     /locus_tag="IYA83_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ8585962.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            51221..51976
                     /locus_tag="IYA83_000500"
     CDS             51221..51976
                     /locus_tag="IYA83_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8585963.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSTLRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            51999..52952
                     /locus_tag="IYA83_000501"
     CDS             51999..52952
                     /locus_tag="IYA83_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8585964.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(53596..53892)
                     /locus_tag="IYA83_000502"
     CDS             complement(53596..53892)
                     /locus_tag="IYA83_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ8585965.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            54247..54603
                     /locus_tag="IYA83_000503"
     CDS             54247..54603
                     /locus_tag="IYA83_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ8585966.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            54613..55512
                     /locus_tag="IYA83_000504"
     CDS             54613..55512
                     /locus_tag="IYA83_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ8585967.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            56029..56760
                     /locus_tag="IYA83_000505"
     CDS             56029..56760
                     /locus_tag="IYA83_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ8585968.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            56905..58932
                     /locus_tag="IYA83_000506"
     CDS             56905..58932
                     /locus_tag="IYA83_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ8585969.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            59040..60137
                     /locus_tag="IYA83_000507"
     CDS             59040..60137
                     /locus_tag="IYA83_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ8585970.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            60444..>60891
                     /locus_tag="IYA83_000508"
     CDS             60444..>60891
                     /locus_tag="IYA83_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ8585971.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        20164 a        10896 c        13000 g        16831 t
ORIGIN      
        1 tttaggtatt agatgcataa ttgctcgatt tctttttcag gtaaattgat atatttataa
       61 catgttccaa ctgaaacacc gcattttgaa gaaataaatt ggatcgtttc ttttttttca
      121 taatagagac gtcggatttt tttgatcgtt tttgcatcga tttttggtct tccgccgatt
      181 tttccttttt tacgagcttc atctaatcca actaatgtac gttctttgat caatgcgcac
      241 tccatgtttg ctaggttttc cattaattgg aaatagattt ttcccatttc gcgcgtatcg
      301 attccttcat caagactaat caagtgcagc ccgcgatctt tgaataattg cgtgagttct
      361 gttaattgcc gcgtagattt gcctaatcga tgaagttggc aaatgactag tgcatcgcct
      421 tgttgcatgt cagcgatcac tgactccaaa tgaacagcag tagaatcatt ttgttccact
      481 tcatatgtct catggaaaat ttcttgacaa ccaaagtcag ataattgatc aacctgagtt
      541 gttaaatcaa cgtcatttat ggttgtgcgt gcataaccaa taattttcat ggtgattcct
      601 ttctagcagt gattttctaa ctggatataa cagtaaaaat gtagcataaa gtacgtcaaa
      661 aagatagttt tttcgcaaat tttccgctgt ctttttttat tggatgatca agcagttttt
      721 ctttatttac gacggattgc gtcaagaatc tcaacgcctt tttctgagcc gacaactact
      781 ttagaaacca tgtttaggaa aagcccatgt tctactaccc caacaagttc atctaaatag
      841 gtagctaagc tttctggctg ttcgatgact tctagatgca gatcaatgat gtaatgacca
      901 ccatctgtgg ttaagacttc tccgttttcg tctgtacgca agacgggttg gaatcttttt
      961 tcttcaaaaa tatgaagtag ctgttgggag ccataaggga tcacttcaac tgggagtggg
     1021 aacttcccaa gtttatcgac taatttagaa gaatcaacga tccaaatcgt ttcttttgag
     1081 tacgtagcga ctattttctc gaaaagcaaa gcagcgccgc cgcctttgat cccttgaaaa
     1141 tcagcactga tttcatcagc accgtctatt gtcaaatcga ctacggggac ttcatcaata
     1201 ctctttaacg gtattcctaa tttttccgct tgttcttttg tcgcatttga agtagtaact
     1261 cctgtgatac gtaaaccttc ttcacgcata cggcgaccaa tctcttctac catataatag
     1321 gcggttgaac cggtcccgag acctacgatc atcccgtcct ttacgaattt cgcggcttca
     1381 atgccagcta attgttttaa attcatcttt gcgtccacct ttttttagtt tgagcatcgt
     1441 gctttcctca ctattttagc gtgatttacg gtaaaaagat accattcttt tttattccaa
     1501 taaagctttt tcaaaaaaaa ttgcatctct tgttgacatg ttttttcatt cgtgatattc
     1561 taacaaaggt gctttatcta caggtttctg tgaagaaacg tgctgactgt ttatcaaagc
     1621 atatttgata aggatgcaga cattgcattt attttttatc acaaaaaaag aaccacctgg
     1681 atgtgtggaa ctagacgcaa atcgtgggaa ggaggaaaca aggaatgcct acaattaacc
     1741 aattagtacg taaacctcgt aaatcaaagg tggaaaaatc taattcacct gctttgaaca
     1801 aaggatataa cagctttaag aaaactcaaa ctaacgtaaa ctcacctcaa aaacgtgggg
     1861 tagcaactcg tgtgggtaca atgacaccta aaaaaccgaa ctcagcttta cgtaaatatg
     1921 cacgtgttcg tttgtcaaac ttaattgaag ttactgctta tatcccaggg attggtcata
     1981 acttacaaga acacagtgtt gtattattac gcggtggtcg tgtaaaagac ttaccagggg
     2041 tacgttacca tatcgttcgc ggtgctttag atactgctgg tgttaacgac cgtaaacaaa
     2101 gccgttctaa atacggaact aaaaaaccta aagcttaatt ttaattatta aacaaatgaa
     2161 acttgcttct actatataga agtaattact atgaacaata tttcggaagg aggagttacg
     2221 gatgccacgt aaaggtcctg ttgcaaaacg tgatgtttta ccagatccta tttataactc
     2281 aaaattagta actcgcttga tcaaccgtgt aatggttgac ggaaaacgcg ggattgcagc
     2341 taacattatc tataatgcct ttgacttgat caaagaatct acaggtaacg atccattaga
     2401 agtgtttgaa caagcaatga aaaacgttca acctgttctt gaagttaaag cacgtcgtgt
     2461 tgggggttca aactaccaag taccagttga agttcgccct gaacgccgcg ttactctagg
     2521 tcttcgttgg gtagtaaact acgctcgctt gcgcggtgaa cacacaatgg aagaacgctt
     2581 agcaaaagaa atcatggatg ctgcaaacaa cacaggtgct tctgttaaaa aacgtgaaga
     2641 cactcataaa atggctgatg ccaaccgtgc gtttgctcac tatcgctggt aagattctct
     2701 ctgtttggtc ggcttttata agtcgacctc agatagtgtc cttattttta gcaactagat
     2761 agtaaagaga ggagaaacaa ggagaatgac cagagaattt tctttacaaa acactcgtaa
     2821 tatcggtatc atggcccaca tcgatgctgg taaaacgact gctactgaac gtatcttgta
     2881 ctatactggt aaaatccata aaattggtga aactcatgaa ggagcttcac aaatggactg
     2941 gatggaacaa gaacaagaac gtggtattac gatcacttct gctgctacta cagcacaatg
     3001 gaagggtcac cgaatcaaca tcatcgacac accaggacac gtagacttca cagttgaagt
     3061 tgaacgttca ttgcgtgtac ttgatggtgc tgtaactgtt cttgatgcac aatcaggtgt
     3121 tgaacctcaa actgaaacag tttggcgtca agcaacaact tatggtgtac ctcgtatcgt
     3181 atttgcaaac aaaatggaca agatcggtgc tgacttctta tattcagtat caactttaca
     3241 tgaccgtttg caagcaaacg ctcacccaat ccaattacca attggtgcgg aagacgactt
     3301 cacaggaatt atcgaccttg taacaatgaa ggctgaaatg tatacaaacg atcttggaac
     3361 agagatcgaa gaaacagaaa ttcctgaaga ataccgcgaa ttagctgaag aatggcgcga
     3421 aaaattggtt gaagctgtag ctgaaactga tgaagaactt actttgaaat acttagaagg
     3481 tgaagaaatc actgaagctg agttaaaaga aggtatccgc cgcgcaacag ttaacgtaga
     3541 attctaccca gtactatgtg gttcagcatt taagaacaaa ggggttcaat tactattgga
     3601 cgctgttctt gattacttgc catcaccgtt agatatccca gctatcaaag ggattgaccc
     3661 taaaactgat gaagaagtag aacgtcctgc agacgacagc gctcctttct cagctttagc
     3721 atttaaagtt atgactgacc cattcgtagg tcgtttaact ttcttccgtg tgtactctgg
     3781 tgttttacaa tcaggttcat acgtacaaaa cgcaacaaaa ggcaaacgtg aacgtgttgg
     3841 tcgtatccta cagatgcatg ccaactctcg ttcagaaatc tctgaagttt acgctggaga
     3901 tatcgctgct gctgttggtt tgaaagacac aactactggg gacacactat gtgatgaaaa
     3961 gagtctagtt attcttgaat ccatggaatt cccagaacca gttatccaag tcgctattga
     4021 acctaaatca aaagctgacc aagataaaat gggtgtggct ttacaaaaac tatctgaaga
     4081 agatcctact ttccgtgctg aaacaaacgt ggaaacaggc gaaacaatca tcgctggtat
     4141 gggtgaactt cacttagata tcatcgttga ccgtatgcgt cgtgaattca aagttgaagc
     4201 taacgtaggt gcgcctcaag tatcttaccg tgaaactttc cgtgctggca ctcaagccga
     4261 aggtaagttt gtacgtcagt ctggtggtaa aggtcaatac ggtcacgtat ggattgaatt
     4321 tacaccaaat gaagaaggtg ctggtttcga atttgaaaat gcgatcgtcg gtggggttgt
     4381 tcctcgtgaa tacattccag ctgttgaaac tggtttgaaa gatgctatgg aaaatggtgt
     4441 cttagctggc tatccattag tagatatcaa agctaaactt tacgatggtt cataccatga
     4501 tgtcgattca aatgaaactg ccttccgtgt tgctgcttct atggcattac gtgcagcagc
     4561 taagaaagct aatccagtaa tccttgaacc aatcatggct gtagaagttg ttatccctga
     4621 agattattta ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg ttgaaggtat
     4681 ggaagctcgc gctggcggcc aacaagttgt tcgtgcgatg gttccacttg ctgaaatgtt
     4741 tggttatgct acaactcttc gttctgctac tcaaggtcgt ggtacattta caatgacatt
     4801 tgaccactac gaagatgtac caaaatcagt acaagaagaa atcattaaga aaaacggcgg
     4861 aaaagctgaa taatttctta aaaccggata gtcctgcaaa gagattatcc aaagagacta
     4921 tatttttaat atagtttcgt gtaaaataat tatcgatgat atgggcatgt ggtgtacctt
     4981 gtgtacatca tatacctatc agacaaataa aaaatatatt gtatttttag gaggacattt
     5041 taaaatggca aaagaaaaat ttgaccgttc taaaccacac gttaacattg gtactatcgg
     5101 acacgttgac catggtaaaa ctacattaac agctgcaatc acaactgtac tatctaagaa
     5161 aaacggcggc caagctatgg cttacgatca aatcgatggt gctccagaag aacgcgaacg
     5221 cggaatcaca atctcaacag ctcacgttga gtatgaaaca gatacacgcc actacgctca
     5281 cgttgactgc ccaggacacg cggattacgt taaaaacatg atcactggtg ctgctcaaat
     5341 ggacggagct atcttggtag tttctgctgc tgacggccca atgcctcaaa ctcgtgaaca
     5401 catcctattg tctcgtcaag ttggtgttcc ttacatcgtt gtattcttga acaaagtaga
     5461 catggttgat gacgaagaat tactagaatt agttgaaatg gaagttcgtg acctattaac
     5521 agaatacgaa ttccctggtg acgatgttcc tgtagttgct ggatcagctt tgaaagctct
     5581 agaaggcgac gcttcatacg aagaaaaaat tcttgaatta atggctgcag ttgacgaata
     5641 catcccaact ccagaacgtg acaacgacaa accattcatg atgccagttg aagacgtgtt
     5701 ctcaattact ggacgtggta ctgttgctac aggtcgtgtt gaacgtggac aagttcgcgt
     5761 tggtgacgaa gttgaagttg ttggtattgc tgaagaaact tcaaaaacaa cagttactgg
     5821 tgttgaaatg ttccgtaaat tgttagacta cgctgaagct ggagacaaca ttggtgcttt
     5881 actacgtggt gttgcacgtg aagacatcca acgtggacaa gttttagcta aaccaggtac
     5941 aatcacacct catacaaaat tctctgcaga agtatacgtg ttgacaaaag aagaaggtgg
     6001 acgtcatact ccattcttca ctaactaccg tccacaattc tacttccgta caactgacgt
     6061 aacaggtgtt gttgaattac cagaaggaac tgaaatggtt atgcctggcg acaacgtaac
     6121 aatggaagtt gaattaatcc acccaatcgc tatcgaaaac ggtactaaat tctcaatccg
     6181 tgaaggcgga cgtacagttg gtgccggtgt cgttacagaa atcaaagctt aattcactta
     6241 agttttaaag taaagcaaca tttattcgga cgtaatcgct cggaacgaag gtcttaggat
     6301 ctttgttccg agctttttta tttattgcag gatcaggaaa aaagacagga gtattataaa
     6361 tagaagaaac acgaaataaa agagtcagta ttttctaaat gaaactttat gaaaacaaaa
     6421 tgttttttac aagaaaaagc cttgctttgt gagtttatat atattataat gacaaaggtg
     6481 cgtttacttt agtgaattgc acagacgtaa tcagaatgat ttcgtcggct aagaaacgag
     6541 aggttgcgac acgcccggac gctttgccac gaacgagcgt gacggaaatt ttcgtggagc
     6601 tatgtctact tttcaaaata ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta
     6661 tccgtttaaa agcgtatgaa caccgtattt tagatcaatc agcggataaa atcgtggaaa
     6721 ctgcaaaaag aactggagct agcgtgtctg gtccaattcc attaccaact gaacgcagtc
     6781 tttacacaat catccgtgcg actcataaat acaaagattc acgcgaacaa ttcgaaatgc
     6841 gtacacacaa acgtcttatc gacattgtga acccaacacc aaaaactgtt gatgctttga
     6901 tgaagctaga cttaccatct ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa
     6961 ataatggagg tgtactcatg accaaaggaa tcttagggaa aaaagtggga atgacacaaa
     7021 tcttcactga atctggtgaa ttaattccag taactgttgt agaagctact ccaaacgtag
     7081 tactacaagt gaaaacaatg gaaacggatg gctacgaagc tattcaagtt ggttaccaag
     7141 atatgcgtga agttttatca aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg
     7201 ctcctaagcg cttcattcgt gaattcaaaa atgttgagct aggagaatat gaagtaggaa
     7261 aagaaattac agtagatgtt ttccaagcag gagacattat tgatgtaaca ggtactacga
     7321 aaggtaaagg attccaaggg gttatcaaac gccacggaca aagccgcgga ccaatggctc
     7381 acggttctcg ttaccatcgt cgtcctgggt caatgggtcc agttgcacct aaccgtgtat
     7441 ttaaaaacaa acgtcttgct ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg
     7501 aagttgtacg tgtcgatgca gaaagaaatg ttatcttgat caaaggtaat gttcctggag
     7561 ctaaaaaatc tttaattact atcaaatcag ctgtgaaagc taaataattg taggaagaaa
     7621 ggaggaacta aggaatgccg aatgtagcat tattcaaaca agatggaagc caaaacggtg
     7681 aaatcacact taacgaagaa atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg
     7741 caatcgtaat gcaacgtgcg tcattaagac aaggaacaca cgcggttaaa aaccgtagcg
     7801 ctgttcgcgg cggcggacgt aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag
     7861 gatctatccg ctcaccacaa tggcgtggag gtggcgtagt ctttggacca acaccacgtt
     7921 cttacagcta caaacttcct aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag
     7981 aaaaagttgc tgaaaacaac ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa
     8041 caaaagaatt caaacaagtt cttgctaact tatctatcga ctctaaagta ttggttgttc
     8101 tagaaaaagg taacgacttt gctgctttat cagcacgtaa cttaccaaac gtttctgtag
     8161 taacttctga taatgtgagt gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac
     8221 aaactgctct tactcaaatt gaggaggtgc ttgcataatg aacttactag acgtaatcaa
     8281 acgcccagtg atcactgaaa aatcaatgct tgccatggat gacaagaaat acacttttga
     8341 agtggacact cgcgcaaaca aaactttagt aaaacaagcg gtagaagctg cttttgacgt
     8401 taaagtcaaa aacgtgaaca tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata
     8461 tgcaggatat acaaaaaaac gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga
     8521 aatccaatta ttcgaagctg ctgaataagc actaatagat aactcattaa attaggaggg
     8581 aaaaacgtgg cgattaaaaa gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt
     8641 tctgattttg ctgaaatcac aacatcaaca cctgaaaaat cattgttaca accattgaaa
     8701 aaccatgctg gacgtaacaa caacggccgc atcactgtac gtcatcaagg tggcggacac
     8761 aaacgccaat atcgcgtgat cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt
     8821 caaacgatcg aatatgatcc aaaccgttca gctaacatcg cgttagtaca ctatgaagat
     8881 ggagtgaaag cttacatctt agcacctaaa ggactagaag tgggtatgaa acttgtttca
     8941 ggtccagaag cagatatcaa agtcggaaat gctcttccat tagaaaacat tccagttggt
     9001 actgttatcc acaacattga aatgaaacct ggtaaaggtg gacaattgat ccgttctgct
     9061 ggtacaagtg ctcaagtact tggtaaagaa ggcaaatacg tattgatccg cttgaactct
     9121 ggtgaagttc gtatgatctt agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa
     9181 caacacgaat taatcaacat cggtaaagct ggacgctctc gttggatgcg taaacgccca
     9241 actgtacgtg gtagcgtaat gaaccctaat gatcacccac acggtggtgg tgaaggtaaa
     9301 gctcctatcg gacgtaaagc accagtttct ccttggggtc aaccagctct tggttacaaa
     9361 actcgtaaca aaaaagctaa atcagacaaa cttattgttc gtcgtcgcaa aaataaataa
     9421 ttactttgac catgtgtcgc acattttgag aggaggttca ccatgggtcg tagtttaaag
     9481 aaaggacctt ttgtcgatga gcatttaatg aaaaaagttg aagctcaagc aggggttgaa
     9541 aagaaaaaag ttatcaaaac ttggtcacgc cgttcaacag ttttcccaag ttttgttgga
     9601 tataccatcg cagtttatga tggacgtaaa catgtaccag tttacatcca agaagacatg
     9661 gtaggacaca aattaggtga atttgcacct acaagaactt atcgtggaca tgctgctgac
     9721 gataagaaaa cgaaacgcta atttgagggg aggataaatc caaatggcag aacaaatcac
     9781 atcagctaaa gcaactgcaa aaacagttcg cacttcacct cgaaaagccc gtttagtaat
     9841 tgaccttatc agaggtaaaa gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa
     9901 caaatctgct ggaatcattg aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa
     9961 ctttgactta gatgttgaaa acttggtagt aagcgaagct tttgttaacg aaggacctac
    10021 aatgaaacgt ttccgtcctc gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag
    10081 tcatatcacg gtagtcgtat cagaaaaata aggagggata atcagtgggt caaaaagtac
    10141 atccaattgg aatgcgtgta ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa
    10201 aagagtatgc tgagttctta cacgaagatt taagaatccg taaatttatt tcaactaaac
    10261 ttgctgatgc tgctgtatca acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt
    10321 caattcacac agctaaacca ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc
    10381 taagaaaaga attaaacaaa ttaactggta aaagaatcca tatcaatatc gtggaaatca
    10441 aaaaaccaga tctagatgca aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc
    10501 gtgttgcttt ccgtcgcgct caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc
    10561 aagggatcaa aactcaagta tctggtcgtc taaacggagc agatatcgct cgttcagaag
    10621 gatactcaga aggaactgtt cctcttcata ctttacgtgc ggacattgat tacgcatggg
    10681 aagaagcaga tacaacatac ggaaaactag gagttaaagt gtggatctat cgtggagaaa
    10741 ttcttccaac taaaaaaaac actgagaaag gagggaaata atcatgttag tacctaaacg
    10801 tgtaaaacac cgtcgtgaat tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga
    10861 agtagcattc ggtgaatacg gtttgcaagc tgttgattca cattggatca caaaccgcca
    10921 aatcgaagct gctcgtatcg caatgactcg ttacatgaaa cgtggtggga aagtatggat
    10981 taaaattttc cctcacaaat cttatactgc caaagcaatt ggggtacgta tgggttctgg
    11041 taaaggggca cctgaaggat gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat
    11101 cgcaggcgtt cctgaagaag tagctcgtga agcgttacgt ctagcttctc acaaattacc
    11161 aatgaaaact aagatcgtaa aacgtgagga aatgggtggt gaatcgaatg aaggttaaag
    11221 aaatcagaga attaaccact gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat
    11281 tgtttaatct tagattccaa ttagcaacag gtcaattaga aaacactgca cgtattaaag
    11341 aagtacgtaa atcgattgca cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat
    11401 ggaaggaggc cattaagcgt atgactgaag gaagaaatca acgcaaagtt tatcaaggtc
    11461 gcgtggtttc agacaaaatg gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc
    11521 accctatcta tggcaaacgc atgaactatt ctaagaaata caaagtgcat gatgaaaaca
    11581 acacagcaaa agttggcgac atcgtgagaa ttatggaaac tcgtccatta tcagctacaa
    11641 aacgtttccg tttgttggaa gtagtcgaag aagcagttat tatctaataa cacgagattt
    11701 tcctatatag attgaatgca aaatctgaaa ggaggataca caacgtgatc caagcagaaa
    11761 gtcgtttaaa agtcgctgat aactcaggcg cacgtgaaat tttgacgatc aaagtccttg
    11821 gtggatctgg tcgtaaaact gctaacatcg gtgatgtgat cgttgctacg gttaaacaag
    11881 caacgccagg tggggttgtc aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa
    11941 aatcaggagc tcgtcgtaca gacggttcat atattaaatt tgatgaaaat gctgcagtga
    12001 ttattcgtga tgataaaagc cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac
    12061 tacgtgaaaa caacttcatg aagatcgttt cactagcacc agaagtttta taatcttgag
    12121 acacttatca aaggaggtgc gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag
    12181 ttatcactgg aaaagacaaa aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag
    12241 ataaagttat tgtcgaaggt gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg
    12301 ctcctcaagg tggaattgtt gagatggaag cgccaattca tgtttctaac gtgatggtgg
    12361 ttgactctac tggtgtagct ggccgagttg gctacaaaga agtagacggc aaaaaagtcc
    12421 gtgtttctaa aaaaaccggt gaagttttag ataaataaaa gtttaaggaa ggagggtctt
    12481 actgaatgaa ccgcctaaaa gaaaaatatc ttaaagaagt aactccatca ttgatggaaa
    12541 aatttaacta tagctctgtt atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg
    12601 gtgttggtga tgcggtatca aacgctaaaa acttagataa agcagttgaa gaattaactt
    12661 tgatcactgg tcaaaaacca atgattacaa aagctaaaaa atcaatcgct ggattccgtt
    12721 tacgtgaagg aatgccaatt ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat
    12781 tcttagacaa attggtttct gtttcactac cacgtgtacg tgacttccat ggtgtaagta
    12841 aaaaagcctt tgacggtcgt ggaaactata ctttaggtat taaagaacaa ttgatcttcc
    12901 cagaagtaga ttatgattta gtagataaag tacgtggtat ggacatcgtt attgtaacaa
    12961 cagcaaactc tgatgaagaa tctcgtgagt tgttgacaca attaggcatg ccattccaaa
    13021 aataattaaa ggaggcgaac tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc
    13081 ctgcaaaatt ttcaacacaa gcttatactc gttgcgaacg ttgcggacgt ccacattcag
    13141 tttatcgtaa gttccatctt tgccgtattt gcttccgcga acttgcctat aaaggtcaaa
    13201 ttcccggcgt gaagaaagct agctggtaaa aagtaaattc gcataagaag gaggtaaaag
    13261 atcaatggtc atgacagatc caattgcaga ttttctaact cgtatccgta acgcaaacat
    13321 ggttaaacat gagtctttag aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat
    13381 cttgaaacgt gaaggtttcg ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt
    13441 gatccgtgtt ttcctaaaat ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg
    13501 tatctctaaa ccaggtttgc gtgcttatgt caaagctgac gaagtaccaa aagtattgaa
    13561 cggtttaggt atcgcaatca tttcgacttc tgaaggtgtt gtcactgata aagaagctcg
    13621 tgaaaaaaat atcggcggcg aagttatcgc ttacgtatgg taatcaagta gaatctacaa
    13681 ggaggtgtct ttaagtgagc cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa
    13741 cagttactca agacggaaac aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta
    13801 ctttttcagc agatatcaaa atgaatatcg aaggaaacga agtaactttc actcgtccaa
    13861 acgacagtaa agaaatgaaa acaattcatg gaacaactcg tgcgaacttc aacaacatgg
    13921 ttgtaggtgt cagcgaaggt ttccaaaaag cgttagaact aatcggggtt gggtaccgtg
    13981 cacaattaca aggatcaaaa cttgtattga acgttggtta ctcacatcca gtagaaatca
    14041 caccaccagc tggagtaact gttgaagtac cagctaacac acaagtaatt gttaaaggtg
    14101 cgaacaaaga agaagttggc gaattagctg caaacattcg tggaactcgt cctccagaac
    14161 cttataaagg taaaggtatt cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa
    14221 ctggtaaata atcgtttaga aaaattgcat agaagccagc agcaggaaaa aggcatttgg
    14281 ctgaagttta ccttcaaaaa aatacatttt tacttgtgcc gcagcttcta tttttcgaaa
    14341 cgtatttact gcttgaaata gtagtacaat aaaaataaag aggtgacaat tgtgattaca
    14401 aaaccagata agaacaaaac acgtcaaaag agacatcgtc gtgtacgtaa caatatctct
    14461 ggtactgctg agcgcccacg cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa
    14521 attattgatg acgtagcggg tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt
    14581 tcaggtggaa caaaaacaga agcagcacaa gctgtcggta aactagttgc tgaacgtgca
    14641 gctgaaaaag gcattaaagt agtagtcttt gaccgtggtg gataccttta ccatggccgt
    14701 gtgcaagctt tagctgaagc tgctcgcgaa aatggactag aattttagaa gaaggaggaa
    14761 taccattcat ggtttatatc gatccaaaac atttggaatt agaagaccgc gttgttgcaa
    14821 ttaaccgtgt aacaaaagtt gttaaaggtg gacgtcgtct acgttttgct gctttagttg
    14881 ttgtcggtga caaaaacgga cacgtagggt ttggtacagg taaagctcaa gaagttcctg
    14941 aagcaatccg taaagcaatt gaagatgcga agaaaaactt agttgaagta cctatggttg
    15001 gttcaacaat cccacacgaa gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac
    15061 ctgctgtaga aggttctggg gttgctgctg gtggaccagt tcgtgccgta ttagaattag
    15121 ctggggtagc agatatcact tctaaatctt taggctcaaa tacaccaatc aacgttgttc
    15181 gcgcaacagt tgaaggtttg aaacaattga aacgtgcaga agaagttgca gaacttcgcg
    15241 gcaaatcagt tgaagaatta atcggataag gaggacaaca ataatggctg aattaaaagt
    15301 tactttaaaa cgcagtatta tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt
    15361 aggtcttaca aagattaaca gttcagtggt taagccttct aatgaagcaa ttaaaggcat
    15421 gatcaatact gtttctcacc tagtggacgt tgaagaaatt taatttagaa aaatcataac
    15481 tagaacatta aggaggtgcc aaacctatga aacttcatga attaaaacct gctgaaggtt
    15541 cacgccaagt acgtaaccgt gttggtcgtg gtacttcatc tggtaacgga aaaactgccg
    15601 gacgcggaca aaaaggacaa aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag
    15661 ggggacaaac accattgttc cgtcgtttac caaaacgagg attcacaaac gtgaaccgta
    15721 aagactatgc agtcattaat cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa
    15781 ctcctgttgc tttagttgaa gctggaatcg tgaaaaacga aaaagctgga atcaaagtat
    15841 tagctaatgg ggaactaaac aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag
    15901 ctcaagaagc aattgaagcg gctggtggat caatcgaggt gatctaatgt ttagactatt
    15961 aaaagatgct tttaaagtaa aagacattag atcgaaaatc ctatttactg tatttgtgtt
    16021 gtttgtattt cgcctaggtg cacatattac tgtaccaggc gtaaatgctg cttcattgca
    16081 aaacctttct tctttgccat ttttcaacat gttgaacttg gtaagtggta gtgcgatgca
    16141 aaacttttcc attttctcaa tgggggtttc accatacatt actgcttcta tcgtcattca
    16201 gcttcttcaa atggacatcg tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg
    16261 tcgtaaaaaa ctgaatcaag cgactcgata tctgacatta gtattggcat ttatccaatc
    16321 tgtcggtatc actgctggat tcaatcaata cgctcaatta ggtttcgtga ataatcctaa
    16381 cgtagcaact tacgtaacga tcggtttgat tctaacagcc ggtacgatgt ttgttacttg
    16441 gctaggagaa cagatcacag aaaaaggtat cggaaatggc gtatcaatga tcatttttgc
    16501 aggtatcatt tctagattgc ctgaaagtat cagagaaatt tatgaagatt actttataaa
    16561 tattgatcct tcagacatat ggaaatctgc tatttttgtg gccattttac tagtagcggt
    16621 cttgttgatc gtgacattcg taacattttt ccaacaagct gaacgcaaga tcccgatcca
    16681 atatacgaaa cgcgtagctg gcgcgcctac aagcagctat ctgccgttga aagtaaatgc
    16741 agctggagtt attccagtta tctttgctag ttcgtttatt gctacgccaa atgctgtttt
    16801 acaagcattg agcggtagat tcggttctga aaactggtac aaagtgatga tggagatctt
    16861 taactataat acagcacctg gtgctacgat ttatacgatt ctgatcgttg ccttcacttt
    16921 cttctacgcc tttgttcaag taaaccctga gaaattagcg gaaaacttgc aaaagcaagg
    16981 aagttatata ccaagcgtac gccctggtaa ggggactgaa gagtatgtct caagattact
    17041 gatgcgttta agtacagttg gtgcagtctt ccttggttta gtggcattgc ttccaatcat
    17101 tgctcaaatg gtttgggatc ttccacaatc aatcggatta ggcggtacaa gcttactgat
    17161 cgttatcggt gtagctttag aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa
    17221 atatgttgga ttcattgagt aagaaagcgt gttgaggttt cccctcaaca cacgttctca
    17281 cacatttatt aggagggaaa acaatgaacc tcattttaat ggggctgcca ggcgcaggta
    17341 aaggaacaca agctgaaaaa atcattgatg cttatggtat tccgcacatc tcaacaggag
    17401 atatgttccg cgcagcgatg caaaacgaaa cagctcttgg cctggaagca aagtcttata
    17461 tggataaagg tgcattagtt cctgatgaag taacaaatgg aatcgtgaaa gagcgactag
    17521 ctgaaccaga tacagagaaa ggcttcttat tagatggctt tccacgtacg ctagatcaag
    17581 caaaagctct ggacgcaatg ctaaaagatt tgaataaaaa aattgatgct gtcatcgata
    17641 tccatgttgg tgaagaaatc ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt
    17701 gtggagcaac ttaccataaa attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt
    17761 gcggtgggca tgaattctat caaagagaag atgataaacc tgagacggtt aaaaatcgtc
    17821 tggctatcaa catcaaaaat agtgaaccaa tcttggctta ttataaagaa caaggtttgc
    17881 taaacactat tgatggtgct cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca
    17941 ttgaaaaata gcacgattgc ccgtagccga cttgcattat gcagttactc ttgtcaagtt
    18001 caataaatta tgatagaata tgtcagtccg acttctagag aatcctctag atgatccatt
    18061 ttgagaattt tgaggtggga actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac
    18121 aagtacaagg aggtactgtg cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc
    18181 gtcgaaactt tgccgaatgc aatgtttaaa gttgaactag aaaacggaca ccaagttctt
    18241 gctactgttt caggtaaaat tcgtatgcat tacatccgta tcttacctgg tgacaaagta
    18301 acggttgaat tgtctccata cgatcttaac cgtggccgta ttacttatcg ctttaaataa
    18361 ttgtactccg taaaactaca ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa
    18421 tgtgcgaaca ttgtaaagta attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa
    18481 atccaaaaca taaacaacgt caaggataat tggaggtgct ataaatatgg ctcgtattgc
    18541 aggagtagat attcctcgtg ataaacgtgt agtagtttca cttacttata tctatggtat
    18601 tggtaacact actgctaaaa agatcttagc tgatgctggt gtatctgaag atgttcgcgt
    18661 tcgtgaatta acgaatgaac aaacagatgc tatccgtgca gaagtagata aattaaaagt
    18721 tgaaggggat cttcgtcgtg aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc
    18781 ttaccgtgga atccgtcatc gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa
    18841 tgcacgtact cgtaaaggcc cagctcgcgc tatcgctggc aagaaaaaat aattcttaag
    18901 tgaaggaggt taaaacttca tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa
    18961 gaatatagaa gcaggtattg cacatatcca ttctacattc aataacacta tcatcatgat
    19021 cactgatact cacggaaatg cattagcgtg gtcatcagct ggttcattag gtttcaaagg
    19081 aagcaaaaaa tcaactcctt ttgccgctca aatggcagca gaaactgcta caaaagcagc
    19141 aatggaacac ggattaaaga ctgttgaagt aacagttaaa ggacctggtt ctggacgtga
    19201 agcagcaatt cgttcattac aagctacagg tttagaagtg actgcaattc gtgacgtgac
    19261 tccagttcct cataatggtg cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct
    19321 cattttgagt tttatcagta acgtcattga acaagacact tttcgttttg aaaggggtaa
    19381 agataagaat gattgagttc gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt
    19441 atggcaagtt catcgttgaa cctcttgaaa gagggtatgg gactacttta gggaattccc
    19501 tacgtcgtat tttattatct tctttgccag gtgctgccat cactaatatt caaatcgatg
    19561 gtgtactgca tgaattctct accatcaaag gtgttagaga agacgtcact caaatcatct
    19621 tgaacattaa aggcttagcg ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg
    19681 atattaccgg acctgctaca gtaactgctg gcgatattat cgttgatagt gatgtagaaa
    19741 ttcttaacaa agatatgtac atctgtagtg tctctgaagg agctacgttc catgctcgct
    19801 taacagtgaa acctggacgt ggttatgttc aagcagatga aaataaaaaa gaagatatgc
    19861 caattggcgt tcttccagtc gattcaatct acacacctgt tcgtcgtgtg aactatcaag
    19921 ttgagaacac acgtgttgga caccgtgaag attttgataa attgacgatg gagatttgga
    19981 cagatggttc aattgaaccg ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac
    20041 atttggatat ttttgtgaac ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa
    20101 aagaagaaac acaaaaagaa aaaatgttgg aaatgacgat cgaagagtta gacttgtctg
    20161 ttcgctctta caattgtttg aaacgtgcag gaatcaatac tgtacaagaa cttacaaata
    20221 aatctgagcc tgaaatgatc aaagtccgta acttaggacg taaatcatta gaagaagtta
    20281 aagcaaaatt gcacgactta ggtttaggtt tacgtaaaga agattaaaac cttttacgaa
    20341 ggagggaaac caacgtgagt tatcgtaaac taggacgcac atctagccaa cgtaaagcga
    20401 tgttgcgtga tttaacaact gatttattaa tcaacgaacg tattgtgact actgaagctc
    20461 gtgcgaaaga agttcgctca actgcagaaa aaatgatcac tttaggtaaa cgcggagatt
    20521 tgcatgcacg tcgtcaagct gcggctttcg tacgtaatga agttgccagc gtgcgtgaag
    20581 aaaatgagga catcgtcatt gaatcagctt tacaaaaact gttcaacgat attgcacctc
    20641 gttacgcaga acgtcaaggc ggatacacac gcattttgaa gacagaacca agacgcggtg
    20701 atggtgcacc aatggttgtc ttagaacttg tctaaaatca cactaataac atcactttat
    20761 agatgattct tttagagtgt tatgatgttg gaagatattt ttccgagtct agctcagcgc
    20821 tgctcctcaa gcgaatatcg cttggggagt aagcactcat cataaagtgt ttttttatac
    20881 cttcaattac ctgtcatgac agatatgttt gaaggcataa aaaaacattt taagcgagaa
    20941 ataattgaaa attttgtctt tttttgatag tgttagtagg aactttagga tgaagaagga
    21001 gtaatcatgg agccaattat tgaattagaa aagataaatt ataaatacca gccagatgat
    21061 cttcgccccg cattgaaaga tgtctccttt acaattgata aaggtgagtg gatcgctatt
    21121 attggacaca atggttcagg aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg
    21181 ccagaatcag ggatcgtgaa agtgggaaat caaatattgg atgaagaaaa tatttggacg
    21241 atccgacaga tggttggaat gatttttcaa aatccagaca atcagtttgt tggttcgaca
    21301 gtagaagatg acgttgcttt tggtcttgaa aatcaaggga ttcccagaga agaaatgcta
    21361 gtgcgtgtga aagatgcact tgagaaagtt agaatggctg agtttgcttc acgcgagcct
    21421 gctcgtttat caggagggca aaaacaacgt gttgccattg ccggcgtggt tgccttacga
    21481 cctgatatca ttattttaga tgaagcaacc agtatgctag atccagaagg tcgtgaagaa
    21541 gtgatctcta cgatcaagaa gatcaaagaa gaaagtcaat tgaccgttat atctatcact
    21601 catgatattg atgaagcagc taatgccaat cggatactag tgatgagaca aggcgaactt
    21661 gtccgtgaag gtacaccaaa agaaattttt tctgctggtc ctgaactgat cgacttaggt
    21721 ctagatctac catttcctga aaaactgaag agcgcactga aagaacgtgg ggttgatgtg
    21781 cccagtgaat atatgacaga agaaaggatg gtggattggt tatggacatc cgttttgaac
    21841 aagtagactt cacttatcag ccgaatacac catttgaaca aagagcctta tttgacatca
    21901 atatgacgat caaagaaaac agttatactg cattagtagg gcataccgga agcgggaaat
    21961 caaccttact tcagcatttg aatgcgcttg ttaagccaac aagcggtaca gtccatattg
    22021 gagaacgaga tattcagccg gatacggata ataagaattt aaagcccatc cgaaaaaaag
    22081 ttggcatcgt ctttcagttt ccagaagcac agctattcga agaaacggta gcaaaagaca
    22141 ttgctttcgg tcctaaaaac tttggtgtca gcgaagaaga agcactagtc ctagcaaaag
    22201 aaacattaga acaagttggg ctggatgaaa gctatttgga acgttcgcca tttgaacttt
    22261 caggagggca aatgcgtaga gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac
    22321 ttgtattgga tgaaccaacg gcaggacttg atccccaggg cagaaaagaa atgatggaga
    22381 tgttttggcg tcttcataag gaacagcaga tcactattgt attagttacg catttaatgg
    22441 atgatgtagc caattttgca gattatgtct atgtgttgga aaagggacga atcgttaata
    22501 gcggggagcc gcaagaggtc tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg
    22561 tacctactgc caccgagttt gcagaagaat taatggctaa agggatgaac tttgctacct
    22621 tgcctttgac agctgaagaa ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc
    22681 atgatgaata aattgatatt tggtcgttat atgcctggag attccttgat ccatcgattg
    22741 gatccccgag ctaaattatt agccagtttt tattttatcg gaatcatttt tttagcaaat
    22801 aattgggaaa cgtacctttt gttggcattt ttcacattac tttctgtctt cttgtcaaaa
    22861 gtagatttga aattttttat ccgaggcatt cgcccattga tctggctaat cctcttcact
    22921 gtagctttgc aggtcttgtt tacaagtggc ggagaaactt attggagttg gggtgttttc
    22981 catatcactg aatttggtgt gatgaacggc ctctttattt tctgtcgatt cgtgttgatc
    23041 atttttatgt cgactctact cacgcttact acaccgcctt tggaattatc ggatgcgatt
    23101 gagtacttgc ttcgtccatt gaaagccgta cggttcccag tccacgaaat ttcattgatg
    23161 ctgtcgattg ctcttcgttt tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac
    23221 gctcagcgcg ctcgcggtgt tgattttggt gaaggaaacc tagttcagaa aatgaaagca
    23281 gtcattcctt tgctgattcc tttatttgtc agcagcttca accgtgcgga agatcttgcg
    23341 actgctatgg aagcaagagg ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg
    23401 cattggcata atagagacac gatggttatc attgcttttg tattattaac tgtgctactg
    23461 atctttttaa gaggataaac gttcagtggg ccgctatcta ttcttttctt gctgatctgt
    23521 ttgcaagaaa ggatgcaagc agtgtatgac aagaaatatt agggtctgtg gcagactaag
    23581 gtcacaggcc tctttctttt atctgaaaag aaagaacgaa aaaattttat agaaaaatca
    23641 ttattccaga taacctttta ggaacaaagg agagaaaaac atggtacgtt acaaagcaat
    23701 cattgcgtac gacggaacaa attttaatgg attccaaaaa cagccaaatg ggcgtactgt
    23761 tcaagaagaa gtagaaaaaa cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt
    23821 tggttcggga aggacagatg ccggagtaca tgcgatcggg caagtaatcc attttgatta
    23881 tccagaggag cgtccgttag aacgaatgcg tttcgcgcta gatacccagt caccagagga
    23941 tattgctgta agacaagtag aaattgtttc agaagacttc cacgcaagat atttggtcaa
    24001 agaaaagacg tatcaatttc gagtggatat agggaaacca cgcagtcctt ttcgacgcca
    24061 ttatgcaagt tattttcctt acccacttga tttggaaaaa attcagcggg cgcttcctga
    24121 tttattaggg actcacgatt tcacctcttt ttgtgcatca ggaagctcga tagaggataa
    24181 aatacgtacg atctacgagg ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt
    24241 tacatttagg ggaaatggct tcttgtataa gatgatacgg atcttagtag gaacgctttt
    24301 gaaaattggc aatggccgat tgcctgaaga tagtatccca gatattattg cgaaaaaaga
    24361 tcgtaatgca gcaggaccta cagcacaccc tgaaggactt tatttgtatg aagtgaaata
    24421 tgaataagag gttatgatag aagtggtcag cttcaagaaa taagaaggta tttacgaaaa
    24481 ttacttctca aaattttgca aaatttcggc ttattttcga agaagctact tctggaacac
    24541 cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaaaga gcaatttcaa
    24601 aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt gataagtgaa
    24661 tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt gttttgattg
    24721 cccttggtgc ggacagtctg aacaggttcc atcgtttctt tattttctac gtcttcttct
    24781 gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc gtgccataaa aggggcgatt
    24841 gcggtcaagg taccagacat aaagaataaa caatgcaata cataggacaa aaaggattgc
    24901 acctatcgta aatccttcag gcaagtaact gaaagtgaca gtatgtttac ctgctggaac
    24961 ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg actggtttcc catcaatttt
    25021 agctttccat cctttgtcat aaggaatagt tgtcaccaac atggaatctt cagcggtatt
    25081 tacagtccca gaagcttttc tgccagtggc ttccatttcg acaccttttt ctttgatctg
    25141 attcattgcg gcttgatagg ctacaacatc tagaccaacg actttaggct ccatgaaact
    25201 tatttctttc gtcccataaa aacttgcagt aaatgtcact gttgtctctt gaggataata
    25261 gccaagatca tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg taccattgac
    25321 agtgatcgtc gcactagaac tttctaattg cgcaaaattg gttgggaaca aacttaagta
    25381 agcttgtgta tttgcaggca catggacttc ccaagtgagc tcttttggct ggttatctgc
    25441 tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca actgattctt
    25501 caaagtaggt gtatagaatg taaaatactg ttcatttaga ccagacaatg tattgatcaa
    25561 gtttgtctga ctggtcaaat tatctgtcac tggctgctca acatcattaa cggacaacgg
    25621 tgcagtaaat gctagtggca atgcattgac attctgatac agtgtatagt cgccgctttg
    25681 accagcttgg cggaagccat atttactgaa cgtgctttcg tttttagaaa tgttatattt
    25741 gattcctgta aacccatcca tcagcaaagt attattgtta taacggatat tcaaatttgt
    25801 ccctcttgaa cggaaaccta atttatccag gtaagaggaa gaattacgat ttcttataga
    25861 agaaaacaaa ctgataccac tgtaaccata gttgatcgaa tcgtttgcag atactggatc
    25921 cagattttcc aagcggtaaa acgagtcgga atcttttttt gtttcattta caagagcttt
    25981 caagtcagga taaggatcag tatataaact gcgggaagca taattccaat catcaagaat
    26041 gccatggatc atactgttcg tattgaccag tgcttcagca gacatgatca ataataacaa
    26101 tatactcaga tgattgaatg aaagtttttt atgctgataa aatcccacag caaaagcata
    26161 aatccctaag aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg tgtagcttcc
    26221 ttcaggtttg atgccccatg ccaccgcaaa gagagcagct acgaccaaaa tgattcctaa
    26281 caacagccca ccatcgtctt gttctatctt ttcccatcct tttcctgcaa gcaggagcac
    26341 taagaaagag aacagatagg cataacggaa taaaaacata ttcggtgcat gcataccatg
    26401 ccagaacaaa ttcaatggaa caagataaaa acttgcgata agtatggcga aaagactgcc
    26461 gtacaaaaat ttatttttca acggtacttt ccgactggca aaataaaaca gacagaggac
    26521 taatggtagc agacctgcgt aaacgaacgg aatcgatccg tattttgtcg tgtcataaac
    26581 accaatcata tttttcatga ttagatccag ataagaagtc gcttctgttt tcaacgtagt
    26641 gatctgactc agtgcctcgc catttgaccg aagatccagc actgctggca atacaatgat
    26701 cattgaagcg ccgcctgcca gtaaagaagt gataccataa ggaacaatcg tttttttata
    26761 ttctgaccag tttgtcaata gacgggcgat aaaatataag aaagaaaata cgccaatcat
    26821 aaaacccata taaaaactgg ataaaaacaa gagtaaataa ctgataaata aaagtttcgg
    26881 ccgtttaaga tccataacac gatgaatccc caaaatcact aatggaagat aaacaaatgc
    26941 atccagccac atgatgatct ccgaatgtgc agtgataaat gagatcaatg cgtaactgac
    27001 gctaagtgca acatgccccc agcgaggaat cttgaacgta cgtgcaagaa accaaaaagc
    27061 caatccggca cagccgacct taagcaaagt cagaacatac agtgcatctg gcatcatctg
    27121 gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct agatagtaag aaattaaaga
    27181 taaataattc aatcctagag aagcattcca tgtataaaaa atactttgct ttccaagaag
    27241 aacatttcgg aagctggcat gaaaattaga aaactgtgaa aaggcatcac ttgccaaaat
    27301 acttctgctg cttccaggat agattccgat acttagataa acaagtgcta aaatcaaaaa
    27361 cggaatcagg aaactagctg ctgcgtagcg tccgttttct ctaaagaatg actttaaatt
    27421 ttttttcata tttattttcc accaaatttc tatgaagttg tattcagttt atcatatcct
    27481 tgtttttctc tgcttaaaaa gagtctagtt ttcacaaaaa aataaaattt agaataaata
    27541 acttcataat ttgttcgtat aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc
    27601 ccacccttaa aaatttttat taaataaaaa accgttcact atctgaacgg tccaagtatc
    27661 attcaattaa acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt
    27721 tttaggtttt ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt
    27781 tgttgagttc atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta
    27841 acaaactttt gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca
    27901 gctcatacca gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata
    27961 caattgacaa ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa
    28021 ggtttttgag tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga
    28081 tagaagaaaa atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc
    28141 aaacgtcagc tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg
    28201 ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata
    28261 ttactgagat tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata
    28321 atggtattgc tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt
    28381 cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca
    28441 attcagttaa tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc
    28501 tggtaactca gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg
    28561 caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat
    28621 acgtcaattc attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc
    28681 tatctatgac tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat
    28741 tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt
    28801 tgtctcaatt gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt
    28861 ctgatcttgc tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac
    28921 cagtagaagg aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag
    28981 caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg
    29041 ctttagctaa aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg
    29101 gagggcaagg actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc
    29161 aagcggatga aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac
    29221 atcatcgcag tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag
    29281 aaatcatggt aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat
    29341 tccgtaacta tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca
    29401 tcaaagtcca cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg
    29461 gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac
    29521 acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg
    29581 tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg
    29641 ttatcagtgg tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag
    29701 aagtaaacgc agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg
    29761 atcaagcagc agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc
    29821 aaggattgac agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa
    29881 cgatgacaga aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata
    29941 cctcaattga cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa
    30001 tcctgatctc taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca
    30061 atgaggacac agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa
    30121 atgaatttgc tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag
    30181 gagatcagcc tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct
    30241 ttctaacaca gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac
    30301 caagtccttt ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat
    30361 cactatgctg gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca
    30421 aacgattgaa gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa
    30481 tctaaacgag atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc
    30541 tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca
    30601 tgatgtgttc aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc
    30661 ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa
    30721 gattttaggt tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat
    30781 tcgtcaaacg acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat
    30841 tgaagaaaat ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc
    30901 tgtaagaagt atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt
    30961 cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa
    31021 agcagaaacg aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca
    31081 aaagctacct tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga
    31141 tcttcgcagt ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac
    31201 agtggttgcc gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat
    31261 ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc
    31321 caatgaagta accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct
    31381 ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca
    31441 agatggagtc gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg
    31501 cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac
    31561 agcgactccg attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat
    31621 cattgatgaa cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca
    31681 attggacagt gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt
    31741 catctgtccg ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta
    31801 tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat
    31861 gaagaatcag gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt
    31921 agtttcgact actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat
    31981 catggatgcg gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg
    32041 cggaagtgat gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga
    32101 acggatgaaa atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga
    32161 attacgtggt cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat
    32221 tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct
    32281 ttggcaaata aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca
    32341 aatagaaaat caggataatc gttatttcga ttagcagact gtgctatact ttatactgat
    32401 gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc
    32461 acctcaagca attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt
    32521 cttactttac ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac
    32581 gattatccat acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg
    32641 aaaaaagact gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc
    32701 tgtattttca gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg
    32761 tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg
    32821 tgccggtttc gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt
    32881 gcaatacggt attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg
    32941 tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc
    33001 ctatgaactc cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga
    33061 gctactcaat ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt
    33121 gaaatctatt gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa
    33181 tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa
    33241 agcagaaatg gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt
    33301 cattaaaact catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca
    33361 cacaatgctt gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga
    33421 atagtatttg gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa
    33481 aatgccagta aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga
    33541 aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg
    33601 agatagatac cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt
    33661 caattagtat tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag
    33721 atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc
    33781 ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct gcatgcagct ttttatcctt
    33841 ctttttttaa tctttttgac acgtaaaagt catttttctg ataattctgg cgataaacag
    33901 aaattatgtt gcaaagtaaa gcggttttct atataattat taataattca ttacacaaag
    33961 ataagaataa cctttgttat aaaacgattt attctgtgta atgtcctttt ttattaagaa
    34021 aaacttccat ttaggaagca gagaggggta ttcgattgtc cgataaccaa ttattagaag
    34081 ttcagaacct acatacagga ttccgactca aagatgacta ttacgatgca gtagatgacg
    34141 tttcatttac tttagacaaa aacgagattt tagcaatcgt tggggaatct gggtgcggga
    34201 aaagtacctt ggctactacg attatgggat tacttgatcc taataacaca aaaattacag
    34261 gtgagatttt atataacgat ctgaatttaa tcgacctgaa cgaaacgctc tataataaaa
    34321 tccgcggaaa tgatatcgga atgattttcc aagatccgtt gtctgcatta aatcctctca
    34381 tgagaatcga agaccagatc aaagaaggac tttcctatca cacaaagatg aatgcagaac
    34441 agcgtcaagc tcgtgcacta gaattgctag aacaagtagg gattcctaat cctgcgagag
    34501 ttggacgcca atatccccat gaactatcag gcggtatgag acaacgtgtc atcatcgcaa
    34561 tcgcaatcgc ctgcaaacct ccaatcgtca ttgcagatga accgacgaca gccctagatg
    34621 taaccatcca agcacagatt cttgatttac tgaaagacct tcaggaagaa acaaaaacag
    34681 ggatcatctt gatcactcat gacttaggag tcgtagcaga aatggctgat cgagtggcag
    34741 tgatgtatgg cggacagttc gttgaagtag caggtgtaaa agaactgttt gatcatccta
    34801 agcatcctta tacacgctct ttactgaact ctataccaca agaaggaaat catgatgcag
    34861 aactccatgt gatcgaaggt gtcgttcctt ctctgaaaaa catgcctcgt aaaggttgtc
    34921 ggtttgcacc acgtattcca tggataccag caagcgatca tgaagaagag cctatccttc
    34981 atgaagtcgc accgaaccac ttggtacgtt gctcctgcta taagcatttc cactttagag
    35041 acgaaaaagg agaggtgtag atgacagaat taatcacaat caaagacctg aaagttcatt
    35101 accctattcg cagtggattc ttcaatcgag taacggatca tgtatatgct gtagatggtg
    35161 tggattttat tatcgaaaaa ggaaagacat atggtttagt gggagagtct ggctctggaa
    35221 aatctactac gggaaaagcc gttgtgggat tagaaaaggt aacagccgga caaatcatgt
    35281 atgaaggaaa agatgtaacg aagcgaagca accgcaaaaa aatgggttat aacaaggatg
    35341 tgcagatgat cttccaagat tcaatgtcca gtttgaatcc taaaaagcga gtgttggata
    35401 tcattgctga acccatccga aattttgaac ggttaagcga tcaagaagaa aagaaaaagg
    35461 taaaaggact gctagatatc gttgggatgc cggaagatgc gctatataaa tatcctcatg
    35521 aattttcagg cggacaacgt caacgtctag gagtagcccg tgctgttgct accaatccaa
    35581 aactgatcgt agccgatgaa cctgtatctg ctctggatct atctgttcaa gcgcaagtat
    35641 tgaatttcat gaaacgaatc cagcaagaat acggcctcag ctatttattc atttcacatg
    35701 atttaggtgt agtaaaacac atgtgcgaca atatcgcaat tatgtacaaa ggtcgatttg
    35761 tagaaatcgg tacaagagaa gacatttata atgacccgcg acatatctat acgaaacgtc
    35821 tgctttcagc tattccgcaa atcgatgtag aaaataggga aagccacaaa gaaaatcgac
    35881 gaagagtaga acaagagtat atccaaaacc aaaaagaata ctatgatgcg actggtcgag
    35941 tctacgattt gcgtacgatc acaactacac acaaagtggc gttgaaagat ggaggtgcaa
    36001 gctaatgtgg aaaacgattc tgcgacgtgt tttattgatg atcccgcaag ttattatttt
    36061 aagtgttctg atcttcttat tagcccaagc gatgccgggg gatccattta ccggtttgat
    36121 caatccaaat caggatccgg cagtgatcga acagatgcga caagcagctg gcttgaatga
    36181 cccatggtat gtccagtact tccgttggat cggaaatgct ttgcaaggcg attttggtca
    36241 aagtttctta tacaaacgct ctgttgctga cttgatcggc gaacggatca tcaacacttt
    36301 atatctatcg atcttgacag ttgtccttac ttatttgatt gctgtgcctc tggggatgct
    36361 tgctggccgg tatcaaaatt caatcttgga taagattgtc gtaatctaca acttctttag
    36421 ttttgctgtt cctttattta tcttcggctt gatcatgcta tttgtttttg gttatcgttt
    36481 aggctggttc ccaacaagtg gatcacaaac actaggtttg caaggtggat ttatcactca
    36541 atggatagac cgtttgaaat atattctgtt accatcaatt acccaagcat ttttggcgac
    36601 tgccgtcaca atccaatatc tgcgaagtga agtaatcgat tcgaaatcgc tcgattttgt
    36661 cagaacagcc cgttccaaag gggtaccgac gaataaagta ttcagtcgac atattttccg
    36721 aaatgcagcg ttgccgatgg cttctcaaat ggggtatgaa atcacttcgt tgattgctgg
    36781 ttctgtagtt atcgaaaaga tttttggtta tccgggagtc ggaaaactat ttattgattc
    36841 catcggccaa cgggactata ccgtgatcac atccttagta ttgattttag gaatcgcgac
    36901 gctagtgggg acattacttt cagatatcat catgagtatc gttgacccgc gtattcggat
    36961 tcaataaaac caatcagaaa ggagaatgga tcgatgagtg aaaaaaacaa tacagcagtt
    37021 caagaaagta ttcctcctat ggggatacga atgattgctc gagaatttaa aaaagataaa
    37081 attgctattt tttcattagg tttgctagtg gttttattat tagtgatctt tatcggtgcg
    37141 atgctgaccg atcaagacaa agtgatgtat gttagtatct tcgacaaata cgctgcacca
    37201 ggaacagttt cgacacaagg acaaaagttc attctgggag cagatgaagg cggacgtgat
    37261 gtattaggac aattgatcat aggtgctaga aactctgtta cgatcggatt tgcgatcacg
    37321 atccttactt cgatcatcgg ggttggatta gggatcatct ctggctacta cggagggatc
    37381 attgacaaca tcttgatgcg gatcgttgat tttatcatga ttctaccgat catgctgatc
    37441 attatcgtct tcgtatcgat tatttccaga tacaatgtct ggtcctttat attcatcatg
    37501 agtgcatttt attgggtagc taaagcccga ttattccgaa gcaagacatt atctgaagca
    37561 agaagagatt atgtcagtgc ctcaaaaaca atgggaacca atgattttaa aatcatgttc
    37621 cgcgaaatca tgccgaatct aagttcatta atcattacta atttgacgat gaactttgcg
    37681 gcaaacatcg gtatcgagac aactttgact ttcttaggtt tcggcttgcc ggcaaatgta
    37741 cctagtttag gtacattgat cggttatgca agtaacggag aagtcttagt gaatcggcaa
    37801 tggatctggt tacccgcatc aatccttatt ttagttttga tgttgagtat caattatgtt
    37861 ggacaagcat tcaagcgttc tgcagatgcg cgccaacggt taggctagta tagaagcgaa
    37921 gggggaaata ggaatgaaga aaacgatttt tggatttgca gcgctgatgt ctgctgtcgt
    37981 tcttgcagca tgcgggggcg gaggtacaga tacctctgga aacaactctg gaggcaacgg
    38041 caataacaaa gaggaattaa cttttccaat tgcaacaaaa aataatgatg atgcaatcga
    38101 agacggcgaa ttagaagctg cggtcgcaac ggacagtcag tttaaaggat tgttccagtg
    38161 ggagttttac caagattcat atgatgcagc ttttatggct ccttctcatg aaccgctatt
    38221 tgcaaacgat gacgatttca gaatcacaga cgacggagct gcaacgcttg atctagatga
    38281 agatacgaaa aaagcgacaa tcaaagtaaa agacaacgtc aaatggtcag atggtgaaga
    38341 tgtaacggcg gacgatgtga ttttccctta cgaaatcatt gggaataaag attacactgg
    38401 tatccgttat gacgacactt tccgtaacat cgtaggtatg gaagaatatc atgatggaag
    38461 tgccgacaca atctccggaa tcaaaaaaat agacgacaaa acagttgaaa tcgaatataa
    38521 agaagtaaac ccaagcatgc ttcaatcggg tggtggtatc tggtcatatg cagcaccaaa
    38581 acatacgtta gaaggtgtag caatcaaaga tatggaatca agcgatcaag tacgtaaaaa
    38641 tccagtgact tttggccctt attacatgag taatatcgtt gcaggagaat cagtagaata
    38701 cttgccaaac gagcactact ggaatggtac gcctaaattg aaaaagatca cgatgaaatc
    38761 tgtaccgatg gcaagtgcaa ctgaagcatt gaactctaaa ttatatgata tgattttcca
    38821 aatgccaaca gatacttacg atacctataa agaaattgct ggctatacaa acttaggccg
    38881 tgaacaaact tcttatactt atctaggatt caagttaggt aaatggaatg cagatacaaa
    38941 gagtgtggaa tacaatccag attcaaaaat ggctgataaa tcattacgtc aagcaatggg
    39001 ctatgcatta gataataatg cagtaggtga acgtttctat gcaggtttga gaagcaatgc
    39061 gacttcattg attcctccag tattccagtc ataccatgat gatgagatga aaggattcac
    39121 acaagatcta gacaaagcga aaaaactatt agatgatgct ggctacaaag atgtggatga
    39181 cgatggttta agagaagaca aagatggaaa taaactaaca atcaactttg cttcgatgtc
    39241 cggtggggaa acagcgcaac cattggcaga ttactatatc caacaatgga aagaaattgg
    39301 tttggatgtc caatacacaa caggtcgtct gatcgaattc aattctttct atgataaatt
    39361 agaaaacgat gatcctgaaa ttgatgttta ccaagctgct tggaatacag gaactgatcc
    39421 aaatccaagt ggactatggg gaccaaatgc tgcgttcaac tacactcgtt atgaatcaga
    39481 agaaaataca aaattgatca acgatatcag ttcaagcaaa gcatttgatg aagagtacca
    39541 aaaagaagca tatcaaaaat ggcaacaata tgcatttgaa gaagcatttg cgattccaac
    39601 gttgttccgt aatgaagtat tgccagtcaa cgaccgtgtc aaagactggg attggtctta
    39661 tgatgtacca aacccatggg caaatgtttc tgttacagca gattcacgtt cataatcact
    39721 aaaaatgacc gaaaaagggc gatgtccttt ttcggttttt tgatattttt ctgtttattt
    39781 ctctaatatt caggtcaagg cgctgatttc atgacttctc caataaacgg tgttcaaagt
    39841 ccagtttctc ggtattaagt caaatttatg caaaaatatg gaaagccatt ttcacaaaat
    39901 tttttcttca tactcgaaac taaacgaatt tttcacaact tctcgtcaat cactaggatt
    39961 attaaagctt tacttacctt ttgaccgaac catttcaaat ttgacaaaaa accagtataa
    40021 taaggaagta tcgaaaaaat atccataaaa ggaatgtgga aaatggacaa ccagctgaca
    40081 aaagaactaa aagaaaacta taacatcgtt tttcacgatc taaatttgct tgaacaagct
    40141 tttacccatt catcctatgt gaatgagcat cgcaatctgc aattatcgga taatgagcga
    40201 cttgaatttt taggagatgc cgttttagaa ctgatggttt ctcagtattt attccgttta
    40261 tatcctcatt tgccagaagg aaaattgaca aaaacacgag ctgcgatcgt tcgcgaagac
    40321 agcttgtcga aatttgccaa agaatgtcac tttgatcaat atgttttgtt gggcaaggga
    40381 gaagagaatt caggaggacg tacgcgacca gcattgcttt gcgacctatt cgaagctttt
    40441 ttaggcgcgt tatatcttga ccagggattc gacacgaccc atgcattcat cgaaaaagtg
    40501 atttttccta aagtcaaagc aggtgctttt tcacatgaga tggatcataa aacaaaacta
    40561 caagaagtct tgcaaaaatc aggtgatgta tcaatcgaat accgattgat caatgaagaa
    40621 ggtccagctc atgaacgagt attttggatc gaagtttatg tagatgatca attgattggc
    40681 actggacaag ggaaatcgaa aaaattagca gaacaagctg cggcggaaaa tgcattggca
    40741 gcactgtaaa agtaagtgag ggaaagcttt gtatttgaaa cgaattgaaa tagccggttt
    40801 taaatcattt gcagacaaga cagtcattga ttttgaaaat agcgtcacag cagtggttgg
    40861 accaaatggc agcggaaaaa gcaatattac agaggctatc cgctgggtat taggagaaca
    40921 atctgctaaa agtctgcgag gcggaaaaat gccggatatc atctttgccg gttccgatac
    40981 ccgtaagcag ctgaatattg cagaagtcac agtgatttta gacaatagtg atcattatct
    41041 gccattggag tacaatgaga tcagtgtaac ccgccgttat cgacggactg gggaaagcga
    41101 gtttttcatc aataaacaaa gctgcagatt aaaagatatc caagaattgt tcttagattc
    41161 tggattagga aaagaatcgt tctcaatcat ttctcaagga aaagtagaag cgattttcag
    41221 tagtaaaccg gaagaccgtc gtgggatctt tgaagaagca gctggtgtct tgaaatacaa
    41281 gcagcggaaa aagaaagcag aacagaagct ttttgaaaca gaagacaacc tcagccgcgt
    41341 ccaagacatc atccacgaat tggaagaaca gttaactcct ttggccgccc agagtgaggc
    41401 agcaaaggag tttttacggc taaaagaaac actgactcaa accgatgtct ctttgatggt
    41461 tgctgaaatc aagacagcaa aaaaagattg ggacaacaaa caagcacagc ttgcgaaatt
    41521 caatctagaa ttaggcaaac tatctgagtc gatccaagaa caagaatcca ttttggcgaa
    41581 gcagcgtaaa gaaaatgcgc aagccgatcg tctgatcgaa aaaaatcagc aagtattatt
    41641 agatctttcg gaaaaactga agcagacaga aggacaaaaa gatgttttgc aggagcggac
    41701 aaagcataca caaaaaagct cgcaagaata tcaaacttca ttagcagaag cacaaaaaaa
    41761 ggttaaacat tttgaaaagc ttcaagaaag cttgatgaaa gcagcagcag aaaaagaaac
    41821 agagatccaa aaagctgaag caaatctcat aaaaacacag caagaattag aaaaatacca
    41881 aaaatcaacg aaagaattat tggcagaatt aagagatcaa tatgttgatt tgatgcaaga
    41941 acaagcagct gtaggaaacg aactgaagta tctagaacgt caatatatac aagaaacagc
    42001 aaaaagcaag cagacgttag ccaagcaatc ggaagtagaa gcatcagtag accgattgat
    42061 cttacaaaaa gaagaattga cacaaaagca ggcgcagcta aaatcatctt taactgaaac
    42121 aaaagagaag ttagaaatga tccaacaaaa cgggaaaaag ttccaagaag cattagcaaa
    42181 agaacagcct aaaatgtatc aattaatgaa tcaagtccag cagctacgtg ctcgtaagaa
    42241 aagccttcaa gaaatccaag aaaactattt tggtttttac caaggggttc gattggtctt
    42301 gcagcataaa cagcagcttt cgggtattgt cggagcagtg gctgaactga tcgatgttcc
    42361 tgcagatttc acgttagcta tcgaaacagc tttaggtggg gcagcccagc atgtcatcgt
    42421 tgaaaatgaa aaagatgctc ggcaagccat cacgtatttg aaacagcaaa gaggaggaag
    42481 agcaacattc cttccactga cgacaatcaa accaagacaa ttaccggctc atatattgac
    42541 acaagcagca gctgtagaag ggtttatagg gattgccagt gaacaagtca gttatcccga
    42601 tcaaatccaa actgtggttc aaaacctgct aggaacgatc cttttagcca aagacttaac
    42661 tagtgccaat gcaatcgcgc aaacgatccg ctatcaatat cgagtggttt ccttagaagg
    42721 tgatgtgatg aatgccggag gatccatgac aggtggagcg aataaacgag gaaaccaagg
    42781 cagtttgttt gtacaaaatc aagaattgaa acagttgact agtgaatttg aagaagcaga
    42841 taaacaactc caagcacaag aaaaaaaagt tcaggagctg caacaagaaa cagctcgttt
    42901 agccgaagaa caagaagttt tgcgtacacg aggcgaacaa ttacgttttg aagaacaaga
    42961 agcgacaaat cagctacaaa acatcataaa tgaacttgaa cgttttgaaa aagaaaagca
    43021 aatctcaact tttgagacac gtgaactgca gcagtttatc gaagattacg aaaagcaaac
    43081 gaacgaattg aaagacaaac aaacagatct tgaatcgcaa cgacaaaaaa tcgacgaaga
    43141 aatcaaatcg ttaagtcaag aaagcgacca gatggaagcc agaagagcac aagtacaaag
    43201 tcaaaaagca caagaacaag cagatcttgc tgtcttgaaa gagcagttta atcatctgca
    43261 gatccagctc cgcggtgccc gagtccaaaa agcagaggca accgaacgtc aagaagcgat
    43321 agaaaagcag ttggcgacat tgacagcaga cttttctgat catgaagtca cagaagaaag
    43381 tttggaaaaa caaattaacg aattatctgc tcagcgagaa acattaaaag ccgagttggc
    43441 aaaagccaaa gagcagcgag atagaaggca aaaggagatc gataagcttg aagcagtctt
    43501 ggcagagcgt aatcgtgagc aaaaagcacg actgtcggaa caatccaaat tagaagtcca
    43561 aaaagaccga gctgaaatga ttttagataa tcatttgacc tatctgcagt cagaatatca
    43621 gctgacattt gagaaagctt cccaagacta tcaagaaaca accgatatcg aagacagtag
    43681 aacaaaggtt tcatccttaa aagaacagat tgaaaaactg ggaccagtaa acttaaattc
    43741 aatcgaacaa tacgaacaag tcagtgaaag acatacattc ttagcaacac agcgagatga
    43801 tctgttagca gcgaaaaacc aattgtttga aacgatggat gagatggatg atgaagtgcg
    43861 gacaagattc aaagaagtat ttgaagctat ccgtcaagaa ttcaaaattg tctttccaaa
    43921 tatgtttggc ggcggtaaag ctgaattagt actgacggaa ccaactgatt tgctgaaaac
    43981 aggtattgag attgaagtac aacctcctgg caaaaaactg caaagtctaa gtttgctttc
    44041 tggaggagaa cgagcactta cagccatcgc gttgctcttt tccattatcc gtgtctgtcc
    44101 tgttccgttt tgtatcttag atgaggtaga agcagcgtta gatgaagcca acgtgaaacg
    44161 ttttggtcgt tacttgagcg atttccaaga tgacacacaa tttatcgtag tcacacaccg
    44221 caaaggaacg atggtcgcag cagatgtact gtacggagtg acgatgcagg aatccggtgt
    44281 atcaaaaatc gtttctgtac ggatggaaga tatcaatgaa gaggcgaaaa tcaacgaaac
    44341 gaaagtgtag gaacgaaaat gatcaaatta gtagcaatcg atttggacgg tacattatta
    44401 aatgaacaaa agctgatttc agatgagaat aaacaagcat tagcacaagc aaaacagcaa
    44461 ggggtcaaga tcgttctatg tacaggacgc cccttagctg cgatggcgca ttatctgcaa
    44521 gaattaggac tggtagatga aggtgactac agcatcactt tcaacggcgg tttggtacaa
    44581 aaaaatgata ctggtgaaat catggaaaag aaagtgatgg aagtatcaga tatccaacgc
    44641 ttatacaaac tggctcagca attggatctg ccactagatg ttttgtctga caatgtggtt
    44701 ttacagctgc ctagtgcccc aaggaagcaa tccttgtaca atgtgttgaa taagctgctg
    44761 caattccaac cagcggatat aagcgatatt acagatgaat ttgtgcttaa taaggcagta
    44821 atcgcttatg accaagaaga attagatcct aaaatcaaag aaataccagc agaattccat
    44881 gaaatgtatg agatcatcaa aacaagaagt atgcttttgg aatttatgcc aaaaggtgtt
    44941 acaaaagctt atggtatttc tttattggca aaagatcttg gtttgaaaca agaagaaatc
    45001 atggcgatcg gtgatgagga aaatgattta ccgatgatcc aatatgctgg actaggcgta
    45061 gcgatggaga atgctgtgcc tttcgtcaaa gaagcagcag atttcgtgac agccagcaat
    45121 gaagaaaatg gcgtagccaa agtgatccaa aaatttgtat tagagtaggg ggagtcaaat
    45181 gggatttttt gataaaatca agcgtgcttt tactggcgaa gagaaaacag aagaagttga
    45241 agtacaggag aagtacgaca agggtttaga aaagacgcgt aaaacatttg gtgaacggat
    45301 gaatgagttg tttgccaatt ttcgaacagt cgatgaagac ttttttgaag aattagaaga
    45361 aacacttatc ggtgcagatg taggttttga aacagcgata cgaattaccg aagcattacg
    45421 tcaagaagtg aaactcagaa atgcgaaaaa atcggcagaa gtccaaaatg cgatcatcga
    45481 gaaacttgtg gatctctatg aagcggaagg aatcaatgaa gtaaatgaac taaatatcca
    45541 agaagacgga ctaacagtta ttttgtttgt cggtgttaat ggtgtaggga aaacaacaag
    45601 tattggtaaa cttgcccatc agttcaaaaa cgaaggtaaa aaagtattga tggcagcagc
    45661 agataccttc cgcgctggag caatcgacca gttagttgtc tggggcgaac gcgctggtgt
    45721 tgaagttgtt cgtggcaatg caggaggcga tccagcagct gtcgtattcg atgctttgga
    45781 aagagcaaag acagagcagg cagatgtttt actagttgat actgcaggcc gactgcaaaa
    45841 taaagtgaac ttgatgaacg aattagaaaa aataaaacga gtaatccagc gggaattacc
    45901 agaagcaccc caagaaacac ttcttgtatt agatgcaacc actggtcaaa acgcgatggt
    45961 tcaagcaaaa caattcaaag aaacaacaga tgtgaccggt cttgttttga caaaattaga
    46021 tggtacagca aagggcggta tcgtacttgc tatccgtaat gaattgcatt taccagtgaa
    46081 attggtcggt cttggtgaag gaatcgatga cttggagccg tttgatccaa atgattttgt
    46141 cgttggattg ttcaaaggat tactaaaaga agaataaccc atgtctaaaa agcaggagct
    46201 gtgacaaaag ttgcagctcc ttgtttgaat gattgttttt aacatagctg tgtttttgat
    46261 gccttcattt ttatacttat ttgtaagcgg ttctcaaaca atttcgttct ttttcttaga
    46321 ctttttgatt ataattagat tcatatgaat tggaggtcac ttaatgatga attttaagca
    46381 attattattg catgtcaatg cgcgcccttt tatcgaccaa gctcgatttg gtatagaacg
    46441 ggaaggacag cgggttgatc ttgcaggaaa tctagcaaaa accgatcatc cagcaatctt
    46501 tggcgatcga tcctatcacc cctatatcca aacagatttt agcgaaacac aaacagagat
    46561 gatcacccct gttaccgatt ctattcccga attatttcag tatctcgctg ctgtttatga
    46621 tgtgactgct cgttctatac cgaaagaaga gatgatctgg ccattaagta tgccacctgc
    46681 cttaccggaa aaagacgaag agatcattat tgcaaaatta aaaaatttcg aagatgtctt
    46741 gtatcgacgt tatttagcaa aagaatacgg gaagcgaaaa cagatggtaa gcggtattca
    46801 tttcaatttt gaatttggcg atgagttgct aagaacattg ttcagccatc aggaagaatt
    46861 tcaagatttt tccgaattca aaacagaact ttatttgaaa acagcgagaa actttatgcg
    46921 ctatcgctgg atgatcactt atttattcgg tgcttcacca atgagtgaga agaactactt
    46981 tttagatgaa tcacatccgc aagaacctgt tcgcagtatt cgcaacagtg cgctgggcta
    47041 tacgaatcat ccaaatgtga aagtttccta tgcttctatg aaacagtatt tagcagatat
    47101 cgagcgaatg atcgaagaag ggaaactttc ggaggagaaa gaattttata caccgcttcg
    47161 tttccgaggc ggaaagaaag tcgcagattt agcaacaaca ggtgttcgtt atattgaatt
    47221 gcggaatatc gatttgaatc cttatgcaag attgggtatc aatccagagc aagttcggtt
    47281 cttacaatta ttcctgatgt acatgttatg gacagaagaa aaagaagact gtgaccaatg
    47341 ggtagcagaa ggtacgactc gaaataacaa agtagcacta gaacaaccat cagatcaaac
    47401 agaatttcat caagaaggta gagaaattct tgaaggaatg aagcaaatgc tggttgaatt
    47461 ggattggttg gattctcttt atttagtaga ggaagcattg actcaaatgg atcatcctga
    47521 acaaacgtta gcagcgaaac tctatcaaga agcacagcta tcgagtcagc aagaagttgc
    47581 tgtggcattg ggacatcaat attataaaga aagccatgaa cgtccctatc agttggccgg
    47641 ttttcgcgaa atggaacttt ctactcagat atttatgttc gatgccatcc aaaaaggtgt
    47701 ccaagtcaaa gtattagatg agtcggatca atttttgcgt ctgcaattcc aagaccatgt
    47761 agaatatgtg aaaaatgcaa acatgacaag taaggacagc tatatcgtcc cacttatcat
    47821 ggaaaacaaa acagtaacga aaaaagtctt aaaagaagct gggttccggg taccaggagg
    47881 cgcagaattt tcatccatgg aagaagcggt aaaagcttat ccgagatttg ccgagcaggc
    47941 atttgtgatc aaacctaaat caaccaatta cgggttaggt atcacgattt tcaaggaagg
    48001 agctagtctg gaagattatc aagcaggttt agccattgct tttcgtgaag acagttcggt
    48061 tttagtagaa gaatttatgc cgggaacgga atatcgtttc tttgtgatcg acggagaagt
    48121 ccaagccatc atgttgcgag tccctgctaa tgttattggt gacagtatcc gaacagtgaa
    48181 agaacttgtt gaagaaaaaa atagtgaccc tttgcgtgga acgaatcatc gtgcaccttt
    48241 agaattgatc caattaggtg aattagaaca attgatgttg aaagaacaag ggttgacgat
    48301 cgaatcagtt ccccaagcca atcaaattgt ttatctaagg gagaactcaa atatcagtac
    48361 aggtggtgat tcgatcgata tgactgatga attttctgaa gcttacaaaa aaatcgccgt
    48421 ttctgcggta gaagcactag gagctaagat tagtggtatc gatttgatta taccagataa
    48481 agaaatcgat cctacaactg ataaaaaagc atatgggatc atcgaagcaa atttcaatcc
    48541 agccatgcat atgcatgttt atccttttgc aggaaaaggc agaagattga cgatgaatgt
    48601 attgaaattg ttatatcctg aagtatttta aaagaaaaag cttttgacag attgtcctat
    48661 ttgatgtcaa tctgatcaaa agcttttttt gcatgtaaaa atttatgtgt tttttcgttt
    48721 taaaataaac gatcccagaa gctttttttc ttttttggtt tttctttttc agctggcgaa
    48781 gagttttctt ctgggtattt tttttcgata tcaatcacag gttcatttac tgcttctttt
    48841 gctgttaaga gcaatccgaa ttcctcagga tttgcagtga cgtaatcgtt gactacagtg
    48901 aaacggctat tgtttttcgt tgctagctgg atatattggt tttggacaga aaggggtaga
    48961 gccccattca agagtatgga agcatctgga tgttttttga gttcttttaa aaagttttct
    49021 ttgttttttg gttggatcat ttcagcaaca gtcatactaa gatagcagcg ttccctgaat
    49081 gtccctaaat atttatgctg ttcttcaggg ttcactaaag gtgtaccata aagtccctta
    49141 tccaaatggc tttttacatc atcttgtgcc ataaaaaata ccctccttct tctctttgtc
    49201 tttcttacga ttatagcata ttcccaataa tacctaaaac atatgaaaaa gcggttgctt
    49261 ttgttcaacg attcacttaa aataagaatg aaaatcattc tcaattagaa aggtggattg
    49321 gatgcgctac ggacgactag tagcattatt agttatttta agtagtattt cattgttcat
    49381 cggagtatcg aatgtacaat tgtcagaact gctccagtta gatgggcaaa gcttacaaat
    49441 cttgctagtg actcgtttgc cacgaacgat ttgtttgatc cttgtgggag cgaccagtag
    49501 tatttgtggg ttgatcatgc aacatttgac ccaaaataaa tttgtttcac caacgactgc
    49561 gggcacgatg gatagtgcgc gtttaggtat tttagtggct atgattttcc tacctggtgc
    49621 atccttgatt gtccgatcct tgacagcatt ttgctttgct tttgcaggaa cattgctttt
    49681 tttatccctt acgcgattgt tcctccagaa aaatcaattg atgcttccgt tgattggagt
    49741 catgttaggg aatatcattg gttcagttgc tacttttttt gcttatcaat tccagttggt
    49801 acaaaatatg acttcatggc ttcaagggaa ttttgcgact gtgatacgag gaaattatga
    49861 gttattgtat gctgttgttc cattgttgct cttggtttgg ctatttgctt accgatttac
    49921 gatcattggt ctgggcgaat cgtttgcagt aaatctggga atagattacc agaagatgca
    49981 gtttttaggc attggcttag tatcattggc tagtgcagtc atgttgatca tggtaggaag
    50041 cattcctttt atcggagtga tcattccgaa tcttgtctct agaatatatg gagatcaagt
    50101 gcacaaaaca attggcgtaa ctgctttagc aggtagtttg ttcttagtat tttgcgatct
    50161 tgtcgctcgc gtgttgattt ttccttatga aataccagtc agtgtgatcg ttggaatcat
    50221 cggtggggct atcttccttt atcttctgat aaggggacga cgatatgctt aaaaaaaagc
    50281 catctatttt tattggtatg cttgttcttg ttttatttgt tacaggaatg tacttgacga
    50341 tccatacata tggaaactgg cattttgcat gtgcattgag gagtaagaaa atttttgcat
    50401 ttatccttgt ctcgctagct gtcagcagtg ccacaatcag ctttcagaca attacgcata
    50461 accagttttt gactccttca gtattgggac ttgagtcgtt atatgtcctt gtccaaacgg
    50521 tccttttctt ctttatcgga ggagtgacga tgcttcagca gacaggtaca gtttttttct
    50581 tattgaacgt tgggctgatg gtcgggctga gtctattgtt atttttcttt cttttgaaaa
    50641 aaatgagagc gaatcttttt ttattactga tgtcagggat gattttaggt acgctttttg
    50701 gtagtgtcag taccttttta caagtcatca tggatccgaa tgaatatgat ttgcttcaag
    50761 gcagattatt tgcaagcttt ggcaatatcc agagtcagta tcttttgcct gcttctttga
    50821 tcattatcgg ctgctggtac ctgttatttc gatatcgaca tgtgctagac gttttccatc
    50881 tcggaaatga acaagcagta aacctcggga tagacacaga tcgttttgaa tttttgattt
    50941 tatgttgtgt cagtcttttg acagggacat caacggctct tgtgggtcca gttacttttt
    51001 tagggtttat tgtcgcaaat atcagttatc gtgtcttttc tacttatcgt catggtcagc
    51061 tatttatcgg aagctttctg gtagctatgc tgtttctgac aggaggacag ctcgcagtag
    51121 agcagatttt cggctggaat acaacagtga gtgtcgtcgt tgaatttgtc ggaggaattt
    51181 attttgtcac aaaattgatt cgcgaaagga agagtatcct atgattgatt tacaaaaggt
    51241 caataaactg taccatggaa aatcagtggt taaagatgta cagttaacga ttcctgaaaa
    51301 gaaattgact gcttttatcg gtccaaatgg tgctggaaag tcaacggtgt tgtcgatgat
    51361 cagtcgtttg ataccaaaag atacaggtga aatctattta gaccataacg aagtaaaggc
    51421 ttggaaatcc aatgaattag ccaaaaaatt atcgacccta cgacaatcga atgaaataaa
    51481 tgtccaaatc accgtgaaag aattagtaga atttggacga tttccttata cgaaaggaaa
    51541 attgacgaca aaagacgagg aaatcgttca ggaatcactg gaacaccttg ggttagaaga
    51601 aatagcggat caatcgattg acactctttc aggcggtcaa ctccaacgtg cgtatattgc
    51661 aatggtgcta gcgcaagaca cggattatat tttactggat gaacctttga acaatctaga
    51721 tatgaatttt gcggttcaaa tcatgcagat tttaaaaaat ttagtacaag aaatcgggaa
    51781 aacaattgtt attgttcttc atgatatcaa ctttgctgca agttatgcag acgaaattgt
    51841 tgcaatgaaa gatggacagt tatttacgca aggaacgacc caagaaatga taaaaaaaga
    51901 tgtattggat gaattatacg aaatggacat acggatttgt gagattgaag gccgcagatt
    51961 ctgtctttat ttctaaaact aacattagga gagaagagat gaaaaaatta gtagtaggta
    52021 tcttgattct agcatcattt ggtttagcag catgtggaaa tagcaacaac accagtcagg
    52081 cagacacaaa aaaaagcagc acacaaactg aaactacatt gacgataaca gatagtaatg
    52141 gagaccaaat cgaggtgcca aataatccag aaaaagtcgt cgtattcgac aatggttctt
    52201 tagatacgat ggatgctctt ggtgttggag ataaggtagt tggagcagct acaagcagcc
    52261 ttcctgagta tctttcatcc tataaaaaag tagaatcagc aggtggaata aaagaaccag
    52321 acttagaaaa aattaatcag cttcagccag atctaatcat catttctggc cgacaaagag
    52381 attttcaaaa agatctaagt caaattgctc cgaccatttt cctttcttta gatgcgaaaa
    52441 atccatggga atctttccag caaaatgtca ctgctttagg ggagatcttt ggtaagcaag
    52501 aagaagctaa aactcaatta gaggaattat ctagtgcaat cgatcagacg aaaaagaaag
    52561 cagaagctac tgacaaaaaa gcactagtca cacttgtgaa tgaagggcaa ttatcagcct
    52621 acggatctgg ttctagattc ggtttcatcc atgatttatt tggctttgaa caagcagatg
    52681 atcaaatcga agcatccact cacggtcaaa gtgtttctta tgaatatgtg ctggaaaaaa
    52741 atccggatat cttatttgta gtagacagaa caaaagctat tggtggagac gacagcaaag
    52801 atgatatttc tgccaatgaa ctggtagcac aaacgaatgc tggtaaaaat cagcagatca
    52861 tctcccttga accagatgtc tggtatttaa gcgggggcgg tttagaatca atgaaactga
    52921 tgatcgaaga tgtgaatcaa gcatttaaat agcataaata acagaggatt aaactctcat
    52981 actatgtaac aataatgttt cttacattct taaatagcaa gagcctatcc tcaaagaagt
    53041 tatcaaaaac catccgtaaa atcgtattga tgattttacg gatggttttt atttatttga
    53101 gaaatctttt ttcaacgtga attcattatc tacatcatac gggttgattc tagaatgggg
    53161 agtggcaaaa aatagtgtga tccatttttt cagcatcaag atcgcctcct ttgatttgaa
    53221 tcaaaggtac aaagcgaaac ttagataaaa cttaaattta gatattttta ttttgtaccg
    53281 atctcttctt taaacgatgt tccatctgct gcttctcggc aataagtcca ctagactaga
    53341 aaatatgaga agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc
    53401 acaatctctt ctaaacgatg ttccatctgc tgcttctcgg caataagccc actagactag
    53461 aaaatatgag aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt
    53521 cacaatctct tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga
    53581 tttcacaacc tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat
    53641 ttttcgataa aaatcggcgg gaagcttact ccaccgtttt aataattgat agaaataact
    53701 ctcttctgct ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga
    53761 agctaatttt ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt
    53821 tacttcaata taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat
    53881 atattccttc atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga
    53941 agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt
    54001 tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg
    54061 ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct
    54121 tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt
    54181 cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg
    54241 atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa
    54301 gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa
    54361 gtcagttgcc aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca
    54421 gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac
    54481 agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt
    54541 ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac
    54601 tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa
    54661 ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg
    54721 cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt
    54781 ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg
    54841 cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt
    54901 gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc
    54961 gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc
    55021 atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat
    55081 ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct
    55141 aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca
    55201 tttttaggat cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta
    55261 gtttttggcc atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc
    55321 tgcagacctt ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa
    55381 cagttgctag acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca
    55441 gcttttcagg cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc
    55501 gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga
    55561 taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc
    55621 agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta
    55681 ttcttttttt gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata
    55741 attaatattg attgaagtct aggcagtcaa gtcacatttt gatagatagg agggggactt
    55801 acgaattatt ttaaaggcta tgacagctgt atttctagat tgtgtttgca tcaagttgca
    55861 ataaaaacta gaaatcaaat cgggctgata atcatttata actaatcgat acataggacc
    55921 aaatataggt ctatgttctt ttatttcata agtatcgtaa aacagaattt tgctatggat
    55981 taatccaatc gactgatatg tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa
    56041 gaaatcgtat taacagcagc aatgatgtta ttcagcatgg tagcaagtac gacttttgtt
    56101 tctgcaactg aagtataccc aaaggaatat aacacagaag gaacgattac atttgaagca
    56161 ggagatgagg gtgtaactcc tcctgttgat ccagagaatc ctgatcccaa taaacctgtc
    56221 gatccatcag atcctccaag ccctggaaca ggaggggcat tatcgattga ttatggatca
    56281 aaattcaaat ttgggactca aaaaatttct actgcggata aaacgtatta tgcagctgct
    56341 gatgtgatga acgatgggtc tagaaaaccg acatatgtcc aagtgacaga taggagaagt
    56401 accttatctg ggtggaaatt aagtgtctct caaccagaac agtttaaaac tgccagtggg
    56461 gatgaactag ttggagcaca gctgaaattt acgaaagggc aggctgtttc tttggttgat
    56521 ccaacatata cgccgcaaac ggtgaattct gaattaacgt tgacaccggg aggaaacaat
    56581 acgttagcaa ttaatgctaa atcaggaaca ggtgtgggga cttgggtcta tcgatttggt
    56641 gcaaatgaaa atgaaaatca agatgcggta cagctttcgg tacccggtaa atctgtcaag
    56701 ttagcgcaac aatattccac taaattagtt tggacattag aagatacacc taacaactag
    56761 tttactgaaa agaactgtta aaaataaata cgtagtcata ttgtcttgcg aggtaatcga
    56821 gcaaaaattg tcaaaggcta ggctaaattt gtttggatac gaatagttca tctaggaatt
    56881 taacgatcag gaggaaaaga tatattgaaa aagaggagga tcgtgctcat tttaagcagt
    56941 ctcttagtga ctaatatctt tccgatcata gggtactctt tggaattgaa tcagttggag
    57001 catggaaata gaagagacga agaaagcaaa ctcacttcga cattcgacag tttaatagaa
    57061 tccaaccaga cacaagaaag cagtgaggat ttaagtattt cgagtagtgg agagaccact
    57121 gacaacgtag aagctgaggg ttcggacaaa caaggactag aagatgcacc aatttctgcg
    57181 gttgagctgc ctgacgatcc caatattatt tctatcgata aaatttttca aacacctatc
    57241 ggtactagca cgtccatatt ggaaaatggg aagttactgc agttgaaccc agcagaaagg
    57301 tctcaaagag gagctatttg gtcaaataag aagattagtt tactctcgga ttttacattt
    57361 aaatcttatt tatacttagg aaatcaacga gatagagcgg gagatggcat gacctttaca
    57421 ttgactggcg atcctagaat ggctagtaat cccagtcaag ttattggatc gccaggtatg
    57481 ggaattggct catattctac tcgttctggc caaccttaca ttgcgaatgc gctctcgatt
    57541 gagtttgata cgtataaaaa tagtggttct tctgatcgaa tggatcgaga gataactgct
    57601 gataatagag ggcatggaca tgtcgctttt gtcacgccta aaccaaataa taataattat
    57661 tctggagagc atactggcgt gacggtagct cctacatttc tttctaatgg cacatggcgc
    57721 ttgttaataa ttaaatggga cgcggagcaa agaagtctta cttatgattt ggaaggtgtc
    57781 ggtcactctt ctcggattat acctgatctc attaggcagt ttggttctac agaagtaaat
    57841 tgggggttta cttcttcaac aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga
    57901 cttccatcga atgtcacatc aacagctgat gtaaaagtca acgatagtga ttatggggcc
    57961 atcgctgaag ctggaggaaa cgataagata actatcagaa atacattgaa ggtcgagggg
    58021 gattttcttg aatataagaa aatgcagact agtatcgacc tcccacaaga attaactatt
    58081 cccgagcata tcttgatcaa tggaaatcaa attgatgcca aagatatctt gataaccgga
    58141 aatcgagtca cgctaaatct gacagattat tttattgctg gagatacgtt aataatcgaa
    58201 ttggagacaa ctttgcagga agtaattcct gaaaaagtac tcactatgca ttttgaatac
    58261 cttgaaaacg ggaatttgat tgagcaatcg aatgaaataa acctaaagat cgcaaagctg
    58321 aaggagatag atatccatgt ttattataaa gataaagaca gtcagattga tattgctgaa
    58381 atgaaaacaa tcactgggaa aataggagag aagtataaag aaactcctat tgatattgaa
    58441 gggtatgttt ttgactcgga ttcagcgaat acagaaggca tcttctccga aaatacagag
    58501 gatatctatt tttattacag aaaaggcaaa ctttatctct caactgctcc tcaatatgtc
    58561 aattttggaa aacataaaat atccagtaga ccattaagtg aattcggaca gttttcaggt
    58621 gggttgaaaa ttatagatga acgggctttt ggaagttgga aattacaatt gaaacaagag
    58681 aaagttctta caaatggaga ttttgagctt ccagaagctc tttcctttgt tacctctgaa
    58741 aatacaacgg ttatagggac atccgctgtt acgattttcg aaagtgatca aaagggtgaa
    58801 agcgatttgt cctctctttt agattctaca gctcatcgtg gaatcagaat agatattcct
    58861 gtagaaaacc aaagaatcgg aacctttgag gggaaactat cttggatact tgcagatgtc
    58921 ccaggaaatt aacaggaaga gaagaatata acaaaattat tgagtttcaa gaagggcaca
    58981 ggtaccatag gctcttgtgt cccacagaga caagattttg ttatagtctt ctcttacata
    59041 tgattaaagg agcgattttc ttgagaccaa aacaacaatt atttataaca ctcctagtta
    59101 ttttaggggc actttttaac aatatatcag tgtcagctag tgcatttaat ttttcagtga
    59161 cgccgataac ttcagagaat caaatcgaca agagaaaaac ttacttcgat cttcaacttg
    59221 atccggatca ggaagtagaa gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga
    59281 aaatcgatat ttctgtgaat agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa
    59341 aaaacgagat tgaaaaagac atgagcctga tctttgatct ggtagattat gtgagctatc
    59401 cacagatagt gacgctaaaa cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc
    59461 cgaatgagcg atttgatggt gtattggctg gtgggatcac attccaagaa atccagacgg
    59521 aaaaggacca aacagaaact aaggatcaaa gcttatcgat tgagaatgaa tacacctata
    59581 ttattgcttt gttgatgcaa cagacactga acgaagttgc tccgaacctt actcttcatg
    59641 aagttaaacc agatcaaatc aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc
    59701 aaaaaaccta tatcaatcaa gtagttattg aaacaaaaat tacaaaaaaa ggacattctg
    59761 aagttttata tcaagaggaa aaagaaggat tgcagattgc gccgaataca aatttttctt
    59821 ttccaactgc tttaaatggt caaccgctaa ccccaggtga atatcacctt acaatgactg
    59881 ttttaggaaa tgaaaatgaa aatggaaaat tttcgagaaa aaaaggaaat actacgatca
    59941 actataccaa tcaatgggta tttgaaaaag attttacaat agacggaaaa gtagccaagg
    60001 aattgaacac gacggatgtt acgattaaaa aagacaataa ttggatttat atcttagtag
    60061 cttcactttt cttattgttt attttattat gcatactttt gtttgttttg agaaaaaaag
    60121 aagagaaaga aaagtaaggt taagataaca aatagtaaat ttttattatt gtaggatggt
    60181 tgggctcttt ttataacaat ataaaatata ccattttatt aataaaaatg catactagta
    60241 aatgatataa ttctaataac aggtttgatc gcgagaaagg ccaaataaaa cgctctccaa
    60301 aatgttaaaa aaaagacagg acgatgtata ttatacaaac aagaatcgct gacaacagtg
    60361 actcggatcc aggaaatcag ttttatcccc agctagttgg tgaatgcatg gaaagtaata
    60421 tacgtagaaa gagctgatga ctaatgaatt atgagttttt tttaagcaaa gaagatcaca
    60481 gaagttattc attacttaaa tatttagaag caagctcaaa tttatcagag tcaatatcga
    60541 atgttcaaga agagttgtcg ctatcgacat ttctcttgaa aaaaacgatc gataagctga
    60601 aacatgattt acaaaagttc gatctagaga aaaatttgcg agtaaccgtc agtgaattgg
    60661 atatatgtct ggaaattaat ggaaaatgtt ctagcaaagc tctgttatct cgttatataa
    60721 cggattcttt atctatgaaa atggtattgg ctttctttca ggaaaaatat atctcagtag
    60781 aaaattttgc taaacagcat cacgtgagtt attctgtggc ttataaagta ttacaaagac
    60841 taaaaagaaa tctaaaaaaa tatcaaattt tttttgaaaa gagaaagttg a
//
LOCUS       DACIFS010000008        59368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.008, whole genome shotgun sequence.
ACCESSION   DACIFS010000008 DACIFS010000000
VERSION     DACIFS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.008"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(182..1492)
                     /locus_tag="IYA83_000509"
     CDS             complement(182..1492)
                     /locus_tag="IYA83_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ8585972.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1504..2922)
                     /locus_tag="IYA83_000510"
     CDS             complement(1504..2922)
                     /locus_tag="IYA83_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8585973.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3162..4730)
                     /locus_tag="IYA83_000511"
     CDS             complement(3162..4730)
                     /locus_tag="IYA83_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ8585974.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4734..5144)
                     /locus_tag="IYA83_000512"
     CDS             complement(4734..5144)
                     /locus_tag="IYA83_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ8585975.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5141..6064)
                     /locus_tag="IYA83_000513"
     CDS             complement(5141..6064)
                     /locus_tag="IYA83_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ8585976.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6061..6939)
                     /locus_tag="IYA83_000514"
     CDS             complement(6061..6939)
                     /locus_tag="IYA83_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ8585977.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6926..8263)
                     /locus_tag="IYA83_000515"
     CDS             complement(6926..8263)
                     /locus_tag="IYA83_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ8585978.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8266..9369)
                     /locus_tag="IYA83_000516"
     CDS             complement(8266..9369)
                     /locus_tag="IYA83_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ8585979.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9443..10951)
                     /locus_tag="IYA83_000517"
     CDS             complement(9443..10951)
                     /locus_tag="IYA83_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ8585980.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10917..11609)
                     /locus_tag="IYA83_000518"
     CDS             complement(10917..11609)
                     /locus_tag="IYA83_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ8585981.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11631..12605)
                     /locus_tag="IYA83_000519"
     CDS             complement(11631..12605)
                     /locus_tag="IYA83_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8585982.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12905..13777
                     /locus_tag="IYA83_000520"
     CDS             12905..13777
                     /locus_tag="IYA83_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8585983.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14235..16433)
                     /locus_tag="IYA83_000521"
     CDS             complement(14235..16433)
                     /locus_tag="IYA83_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8585984.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16665..17657
                     /locus_tag="IYA83_000522"
     CDS             16665..17657
                     /locus_tag="IYA83_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8585985.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17717..19138
                     /locus_tag="IYA83_000523"
     CDS             17717..19138
                     /locus_tag="IYA83_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ8585986.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19131..20630
                     /locus_tag="IYA83_000524"
     CDS             19131..20630
                     /locus_tag="IYA83_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ8585987.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20669..22063
                     /gene="uxaC"
                     /locus_tag="IYA83_000525"
     CDS             20669..22063
                     /gene="uxaC"
                     /locus_tag="IYA83_000525"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ8585988.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22222..22905
                     /locus_tag="IYA83_000526"
     CDS             22222..22905
                     /locus_tag="IYA83_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ8585989.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22907..23743
                     /locus_tag="IYA83_000527"
     CDS             22907..23743
                     /locus_tag="IYA83_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ8585990.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23754..25508
                     /locus_tag="IYA83_000528"
     CDS             23754..25508
                     /locus_tag="IYA83_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8585991.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRIYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25784..26125)
                     /locus_tag="IYA83_000529"
     CDS             complement(25784..26125)
                     /locus_tag="IYA83_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ8585992.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26193..28364)
                     /locus_tag="IYA83_000530"
     CDS             complement(26193..28364)
                     /locus_tag="IYA83_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ8585993.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28575..29432
                     /locus_tag="IYA83_000531"
     CDS             28575..29432
                     /locus_tag="IYA83_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ8585994.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29503..30360)
                     /locus_tag="IYA83_000532"
     CDS             complement(29503..30360)
                     /locus_tag="IYA83_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ8585995.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30345..33047)
                     /locus_tag="IYA83_000533"
     CDS             complement(30345..33047)
                     /locus_tag="IYA83_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ8585996.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33060..34349)
                     /locus_tag="IYA83_000534"
     CDS             complement(33060..34349)
                     /locus_tag="IYA83_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ8585997.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34499..35545
                     /locus_tag="IYA83_000535"
     CDS             34499..35545
                     /locus_tag="IYA83_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8585998.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35547..37700
                     /locus_tag="IYA83_000536"
     CDS             35547..37700
                     /locus_tag="IYA83_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ8585999.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38173..39624)
                     /locus_tag="IYA83_000537"
     CDS             complement(38173..39624)
                     /locus_tag="IYA83_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586000.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39621..41345)
                     /locus_tag="IYA83_000538"
     CDS             complement(39621..41345)
                     /locus_tag="IYA83_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ8586001.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41338..41952)
                     /locus_tag="IYA83_000539"
     CDS             complement(41338..41952)
                     /locus_tag="IYA83_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ8586002.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42297..43754)
                     /locus_tag="IYA83_000540"
     CDS             complement(42297..43754)
                     /locus_tag="IYA83_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586003.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(43795..44715)
                     /locus_tag="IYA83_000541"
     CDS             complement(43795..44715)
                     /locus_tag="IYA83_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8586004.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44727..45674)
                     /locus_tag="IYA83_000542"
     CDS             complement(44727..45674)
                     /locus_tag="IYA83_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8586005.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46153..46872)
                     /locus_tag="IYA83_000543"
     CDS             complement(46153..46872)
                     /locus_tag="IYA83_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ8586006.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(46921..48567)
                     /locus_tag="IYA83_000544"
     CDS             complement(46921..48567)
                     /locus_tag="IYA83_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ8586007.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            48746..49336
                     /locus_tag="IYA83_000545"
     CDS             48746..49336
                     /locus_tag="IYA83_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8586008.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49428..50933
                     /locus_tag="IYA83_000546"
     CDS             49428..50933
                     /locus_tag="IYA83_000546"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ8586009.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51017..52369)
                     /gene="celB"
                     /locus_tag="IYA83_000547"
     CDS             complement(51017..52369)
                     /gene="celB"
                     /locus_tag="IYA83_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ8586010.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52369..52887)
                     /locus_tag="IYA83_000548"
     CDS             complement(52369..52887)
                     /locus_tag="IYA83_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586011.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(52903..53229)
                     /locus_tag="IYA83_000549"
     CDS             complement(52903..53229)
                     /locus_tag="IYA83_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ8586012.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53242..55221)
                     /locus_tag="IYA83_000550"
     CDS             complement(53242..55221)
                     /locus_tag="IYA83_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ8586013.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55242..55556)
                     /locus_tag="IYA83_000551"
     CDS             complement(55242..55556)
                     /locus_tag="IYA83_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ8586014.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55724..56017
                     /locus_tag="IYA83_000552"
     CDS             55724..56017
                     /locus_tag="IYA83_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ8586015.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56095..57207)
                     /locus_tag="IYA83_000553"
     CDS             complement(56095..57207)
                     /locus_tag="IYA83_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ8586016.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57594..57773
                     /locus_tag="IYA83_000554"
     CDS             57594..57773
                     /locus_tag="IYA83_000554"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586017.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            57967..58137
                     /locus_tag="IYA83_000555"
     CDS             57967..58137
                     /locus_tag="IYA83_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8586018.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58561..59301)
                     /locus_tag="IYA83_000556"
     CDS             complement(58561..59301)
                     /locus_tag="IYA83_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ8586019.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17757 a        12143 c        10386 g        19082 t
ORIGIN      
        1 agcttcgcaa gtggttcttt ttaacgactt agttatctta tcacgtctaa aaacatgtgt
       61 caactctttt ttggaatttt cataaaatgt ttcaatgaag cgcgacaacg ttgtttaata
      121 tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc ttattataat
      181 ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata gataaatcac
      241 agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt gcagttattt
      301 ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact aaatcaaaag
      361 ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg atgtgtatgt
      421 ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct tgccaacttt
      481 ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc cagtccatta
      541 tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg atattttcga
      601 tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac tgaaaaaaat
      661 cgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca atagttacac
      721 cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt cgattgactc
      781 ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca caggccaaat
      841 cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct gtacttggtc
      901 catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa gtgatttgca
      961 agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt tctgcatgat
     1021 acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga agtccctttt
     1081 gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc tcccaccaat
     1141 ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc gccgagagaa
     1201 cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta tcgatttctg
     1261 gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag ataagatgac
     1321 tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata aatacaggta
     1381 agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca tcttgttgag
     1441 tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc aatacgtttc
     1501 tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt ttgaacgatt
     1561 cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg ctggtaacaa
     1621 gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc cacctgttgt
     1681 agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg cacatgcttg
     1741 tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc ttaagcctgt
     1801 cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca aattgaataa
     1861 accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat ttgttatatg
     1921 aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata cgcttgcttc
     1981 ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta ctactgttcc
     2041 gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat cattgaataa
     2101 ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt caacaatgac
     2161 taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag cagatacttt
     2221 ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca tatatgcgac
     2281 aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg aaacgacttg
     2341 ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg tagttgatcc
     2401 gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat taggatttga
     2461 gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa aaatcccata
     2521 aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat gttggacgaa
     2581 tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc tgatccaagg
     2641 aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa ccatcacatc
     2701 agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg cccctgaaac
     2761 gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta aaataaaaca
     2821 gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt cctttttttc
     2881 tttttttatt gtatttgctg taacagcatt ttgtgaaagc atttgaattc ctcctacctc
     2941 ttctagtaaa cgttttcaaa attataactc tttttttctt gtttttatat tcacaatgtt
     3001 ggctttttat tgcattatct tgacagataa aaaacaaaaa ataatctaaa aacattgatg
     3061 cataagattg ataacttttt ttacataaca atatatacaa acaaaaaaat tcactaacta
     3121 gtgaaaataa gttagtgaac ctcatttatt aaagaattaa attacagatg atcttttcca
     3181 tcatacttga aaaactcaaa atcagcataa cttcctcttt ttttatttag atcatgcact
     3241 gctatcccga taaaatttcc agtaaaccct cctgagagta tacacatgtt tttttgcgga
     3301 cctactttct tccattcttc agttgatacg tctttcaaat gaaactgtgc tgtctctttc
     3361 tttacagtga ctttcaaaaa agtcttcttt tctgtgagtg gaatcgtcca atcttctatt
     3421 tgtgctactc cgttttctga ttctatcata cgtaggcatt ttcccttgtc ctcattccaa
     3481 gtaacataac aataaatata attggattca tttaagtaca gtaccagtcc agccatctga
     3541 ttaaaattat taggcatgta ttcaaatgct gtctccgcat caaaagtaaa atccgtttgc
     3601 cgaatagcta acaaatgatg ttcgaaggta gattgaagag attcaccgga aatcagacgt
     3661 aaaaaacctg gtctacattt tgtatcacac caatcagcat ttggcaaaat gcgtctggca
     3721 ttccagttct tctttagctc tccttcaaat gtatccataa actgatgatc tttttcttgt
     3781 tgtacctctt catttgtctc aatcattatt tcttccatcg gagttgtctg gctatcttta
     3841 agtcttaacc aatcgtcgga cgtccaatac acttcttgaa tagcggtttc tcttcccaaa
     3901 atagccgcac cgtttaatgg tctggtacaa agatatgtca tgtaccatct tcctgacggg
     3961 gtttgtacta agctcccatg tcctgtacat tgcaatgtag aagcaggttt gtcactagct
     4021 gtcagcatag gaaaaccagg atcaagctcg taaggaccag taatattctt tgctcggcaa
     4081 actgtaactg aatgtcctct tcctgtaccg ccttctgccg tcatcaaata ataatacccg
     4141 ttacgaaaat acaaatgtgg tgcctccgtt ttcgctaatt ctgtacctgc aaatatttta
     4201 tagatgggac catcaagctt cttcttctct gcatcatatt cttgtaatac gataccgctg
     4261 gatttattcg gtgtgtccag acgataatcc catagttcat tgacaagcca tttttttccc
     4321 gtttcaagat catggaacaa agaaggatca aagccgttac tatttaaata aattggttcg
     4381 gaccaaggac ctgtgatctc agatgcagtg atcaaataat tgtggacatc tttaaacgga
     4441 cggcctgcac ttttgacatc tgtgtaaaca agataaaaca atccatcagc ataactgatc
     4501 tgtggcgccc atatacttcc atttgtagga tttccttgca aagatacttg agtggtcaaa
     4561 atatccgttg tatgtgacca gttgactaaa tttttcgact catatactcg gattcctgga
     4621 agccattcaa aagaggagac aataatgtaa taccgctctt tcactcttaa tatattagga
     4681 tctggattga atcctcttaa aataggattt ttcactttga tagtctgcat gattcaggct
     4741 ccttttctca ttcttactcg atataaactg aatgccccag ctgcgaaata aaaaaatgca
     4801 aaaggaaacg caataaactg agtgccaatc aacaatatga atcctgttat aaaatacaaa
     4861 ccagtggcag ttgttaagta ttttccttta gcagtcaaaa acaatgctac tgctaaaaaa
     4921 gcaagtgaaa taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat
     4981 gtattttcta aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac
     5041 tctgacgcag acatattact taaagtaact gctaacccgc caaagaaaat caaagataga
     5101 ctgccaccga ccattcctag caatagttcc cattttcttt tcatttctga accccctgct
     5161 tttctaaatg tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag
     5221 agaatttgac agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa
     5281 catgcccacc tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc
     5341 ctaaatagac gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc
     5401 ctgttttttg atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta
     5461 agctatggat atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg
     5521 ttccttcaat ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg
     5581 gtgtacaaaa aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat
     5641 cctgataacc agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag
     5701 caacagcctg tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta
     5761 atacgacatt cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac
     5821 cgtcaagata ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca
     5881 caagtttaaa attggataat ctgctttcta tatattcatc atatcttcct tcttttacta
     5941 tcaagacctt tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag
     6001 tttcaaaatc cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc
     6061 tcataaatgc tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg
     6121 accgcctgtt aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt
     6181 gtccagccct tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc
     6241 aactaagcta agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc
     6301 tgtcaataga tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa
     6361 aagtatttcg atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa
     6421 tgctgtcacc caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg
     6481 agtatctgtg tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg
     6541 cattccccat aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat
     6601 acctccgcgt tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt
     6661 cagtacttct gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt
     6721 cccgactgaa aaatctccgc cgtgagaatg caggtaaagg accggcggaa acggtccttc
     6781 tttttctgga taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata
     6841 gtattcaagc ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg
     6901 tctgctagat ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa
     6961 ccagttcatc catattttct gattgatttg attggaattt ctcaatactg atttctttag
     7021 tattttctaa tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa
     7081 ccttacccgc aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca
     7141 tagcaggaac accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga
     7201 tttcttgtat gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc
     7261 ttgccgtgat attcgaaaaa tggatccttc tgaatgctgg tgtccgttca tctatcggat
     7321 aagccttttt ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat
     7381 tcagtataaa tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa
     7441 cgatcccgcc tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga
     7501 tacaatttga aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac
     7561 cgccatgtcc atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt
     7621 cgtacgtatc ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc
     7681 aattactaat acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat
     7741 ccgctgggtt caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga
     7801 tcgtccaact tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat
     7861 ggaaactcat caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat
     7921 gccaccattt ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta
     7981 cattttgagc atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat
     8041 gaacaacagg ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat
     8101 gaagttcgac attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa
     8161 ctacggtccc gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat
     8221 tcaattcatc aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac
     8281 ttcaatcaat gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc
     8341 actgatgtag taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg
     8401 acagttttta ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa
     8461 tccttgatgt gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg
     8521 tattccttta gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc
     8581 tttccgtttt gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg
     8641 aagtaatgca gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc
     8701 atgaagtaat tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca
     8761 aaaatgatgg gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca
     8821 tgcatgaatc agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg
     8881 tctcaaaaca tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa
     8941 aggagaaccc atatataagc catccaacca aatttgataa ggataaattt ctttatgcca
     9001 aaaagctcct tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc
     9061 ttttttatat ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt
     9121 tacatggtcg atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac
     9181 gaaatagtcg atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca
     9241 aagtaattgg aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca
     9301 cttttcttca tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca
     9361 gttttccatt atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa
     9421 gtatttgtcc caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt
     9481 gtgtgattcg atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt
     9541 cgtcaagctg tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg
     9601 atcctacttc attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt
     9661 cttcattttc tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag
     9721 agaacgtcac ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat
     9781 ccatatcgat aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac
     9841 cgccagtcat caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt
     9901 tcttcaattg tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac
     9961 catttccttc tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag
    10021 gcactaattc catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca
    10081 ccattccagt gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt
    10141 gggtaactgc aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca
    10201 aagctttcat atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag
    10261 cccagccatt tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat
    10321 cactaggcgc acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt
    10381 tttctgtaaa ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc
    10441 ctagattatc caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta
    10501 tcggtacgcc gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt
    10561 gacttaaatt tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta
    10621 acgcattgcg gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta
    10681 gttcatcaga agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt
    10741 tgaatgtcag ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg
    10801 gtgaagcatg atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg
    10861 aagaactgtt acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa
    10921 gacctaattt tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata
    10981 tttttgtttt cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata
    11041 gaatttgctg tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca
    11101 cctggcattg tttggatgat catcagataa atccctacca tacttaatat caacactaat
    11161 gagtaatgaa gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct
    11221 ttccaatcat aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac
    11281 gcagcaatat acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt
    11341 aaaacaatcc cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct
    11401 ttaaaatatg tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt
    11461 ctaatcactg caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg
    11521 atgactaaac cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt
    11581 ccgtttattt tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg
    11641 ccgtcttctc ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg
    11701 acccctttaa acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct
    11761 gtataaatat aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag
    11821 tacatatgct cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg
    11881 attactggac gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca
    11941 tccatttctg cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg
    12001 atcgtagacc agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat
    12061 tcaggggatg ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat
    12121 acccctccgt caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg
    12181 aagtgaggga tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca
    12241 tttaatagta aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac
    12301 aagatcaacg tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa
    12361 tgtttgaaac cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa
    12421 gcaataacga gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc
    12481 agaatcatca catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt
    12541 ctgagctttt cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt
    12601 tccataagcc cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac
    12661 tatctgaatt acgtatataa tcatttttac cgtaagtaaa aacgcttaca tagcaatgat
    12721 ttttgtgcta tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac
    12781 cttctatttt tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac
    12841 cgatgataaa ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat
    12901 ctatatgaaa aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt
    12961 tctaattttg ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt
    13021 tgcttctaca caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt
    13081 agacgcgtat cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat
    13141 tggtgttaca ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg
    13201 tctttccaga aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat
    13261 gttgttcagc ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa
    13321 ttcttactgg gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag
    13381 aaactttttc caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac
    13441 agattttggt gtatttttca aaattatgct gccattagcg ttaccgtcta ttgctactat
    13501 ctcgcttttt tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa
    13561 cgattcatct aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg
    13621 gatgcaagca gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc
    13681 tgttatcgtt attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt
    13741 tgtcaaagga gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt
    13801 agttgtgtca gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt
    13861 aaatttttgt gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc
    13921 agaggttgtg aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg
    13981 acgagtaacc cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca
    14041 aaattatgcc ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac
    14101 tgtgacaaga cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca
    14161 taaatacaaa tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa
    14221 aacagcctct tgattcacaa atattttttt cgatattctc ccggcgtaat cgattccttc
    14281 tttttaaaga aacgaataaa attttgcgga tttcgatatt gcaatctttc tgcaatctct
    14341 tttacggaga ggttagtctc tttcaaccat ttttttgcta cctctaaacg atgattttga
    14401 acaaaatcac caaatttttc tcctgtttcc tttttaaata cattacttaa atagttaggg
    14461 ttataatgca acctatcagc gatcacatct aaagacagat cacgatcata ttctgtgtag
    14521 acgatatgca taatattctc cgacaaattt ttaaattcat gatcagtgat tgcttgagag
    14581 cattctgtga ttggcagaac taattgttct aaaacaagtt tttctagttc tttgggatga
    14641 taattgttca gtgcttttac ataaatttgt ttcatgttat caaagttttt tgaatctact
    14701 cccaataact gtcctaattg aacgagttca ttgatcaagc ggaccgaaac tacttcccga
    14761 ctcaaaggat ttttattttg tgcaaacaat gcatcaatca attcatgtac taaattcttc
    14821 acttcctgat ttccagaacg aatggcttca aacaatcgat tttgttcttc tatcggatat
    14881 ctaataagtg ttttttcatg agcagcagga acgatttcct gataaaaaat aattgaactt
    14941 ggaccagtat ttacacgata atacagggct tcttttgccc gatcaaccga ttgcttgctt
    15001 tcattcagcg tttcgaaccg gttactgatg cctatactga tagttaaatt caagtattct
    15061 tttacacgtt tttgaatgag tcggcaatac tccatgactt ttttgcttgc ttctgcatca
    15121 tctttatcca ttcgataaat agtggcttgc atttcttcat tcaaaacgat cggcagcatt
    15181 cgattttcct ttggcacaat ttccgtgatc atgttgttga tcgctaaaag tagcaaatca
    15241 cgctccccac cgtgactatc ttctaacaga tcgatttgaa ccaaagctgt ataatagcag
    15301 tcattttgag aactataacc aaattgctgc aaccgctgat ctagttctcg cctttctact
    15361 cgatttctaa ataaactaag tacaaatagc gtttccagct gtggcttttg ggtaactaag
    15421 tgtgcactta acgcttcttt ttctcctatg atccgactga ctgattgagc gaccaaatct
    15481 agttctttcc cactgaatcc ttttgattta agattcagac gttcctgtat ttgagaaatc
    15541 ggacgggcaa atctgtccga aaataagtag gaaagaagac caatgagtcc taccagtgtt
    15601 aaagacactg caactagccc gattctcaga ttgcggatca ttgcgccaat tgcactagag
    15661 tcaacttcta atctgtaaat ccaattatta taatctgatt tcagtactat atacgtttta
    15721 tctttttctc ttatcacttt cagctgattg taatctagat ccacgtccgt taatacagtt
    15781 ggttgctctt tattttttac tgtctgagta gtaaaaaggt tgccaacttc gttttcaata
    15841 gaaagtatgt cttctccact ttcaccaaca acttgttcta ttgtatgttt ctctatcgta
    15901 gcaattccca atgcatattt ttctttttca aacattggca aagaaatcac catttcgatt
    15961 cctttgtttt tcttttgcca aaataaatta tttttgttat ttatgtactt ctgttgatac
    16021 ttttcaattt cctcttcggt cagttgactc aacgatccat tgattactcc ccatttacca
    16081 gccaaactga ctaaatcata attattatca tccataacaa tcgtttctat aaaatttagt
    16141 tctttcctga tttcagaata agtggagtaa tctgtatatg tcagcggatg cttgaatatg
    16201 tccgagaaat ttttggtcga accatacgta gaaaacgaat attcaatcgt tctaattttc
    16261 cattcaacgt tagttttaac ttgcctgagc aaattctttt tgtctgttaa ataactcgat
    16321 tcgatcgatt cataggtatt ctggtaaatg aataaactaa atcctatgac aagtacggct
    16381 cctaataaaa acatcggtaa aaatatttta taaaatactg tacttttctt cattttcttc
    16441 ctctcctcct tttaaaataa aaggctttca tttttctgta aaattgatac tcatctaatt
    16501 aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc aagtaatttt
    16561 taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta tcgtaatatg
    16621 atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta acgatcaaag
    16681 atgtcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg aataacagca
    16741 gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa atgaattatt
    16801 cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc ggactatttt
    16861 tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt gcaggcatcc
    16921 attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt gttgatttaa
    16981 cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt gatgatgatc
    17041 agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc aatcgtcaca
    17101 tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt atgcaggcag
    17161 tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga aagaaaaatt
    17221 ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa gcgagcatcc
    17281 ctgtcaatcc agcctatttt ttaacaggag attacagctt agaaagcggc ctccaaaaca
    17341 tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc aacgacgaca
    17401 tagcgattgg tgcattgaga gctgtacatt tagctggcct ccgtgttccc cagaacattt
    17461 ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg cttaccaccg
    17521 tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta gatttgatcg
    17581 aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc cttcgtgagt
    17641 cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt gtgcataaca
    17701 cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa acgaagcacc
    17761 tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg attggcagat
    17821 tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc aacctttaga
    17881 acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca ttttacaagg
    17941 actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag aacaaatgat
    18001 caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg acttggccta
    18061 tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata atttaacaga
    18121 tactccccca cagagcctcc cgggcaaact gacagccttt ttataccgac gtttcaaatt
    18181 aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg gcgaacgtct
    18241 aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt ttgttgagtg
    18301 gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc caggttatcc
    18361 tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat tgatggtcaa
    18421 agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga atcaactacc
    18481 attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt atcgtgaacg
    18541 taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt tgttggcgga
    18601 attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg ttgatcagtt
    18661 attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag ccatttatgc
    18721 tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag aagcgatttc
    18781 attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga agtatatcga
    18841 agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac ttcttatgta
    18901 tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat ttactgatgc
    18961 ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc tttggggaga
    19021 agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg caaagatcga
    19081 tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc atgcttgaaa
    19141 ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca ttaaccccta
    19201 ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt gaagatatcc
    19261 cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg atcaaatatg
    19321 gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg cacactcata
    19381 atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat gtccatccaa
    19441 gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat ggaaaagtag
    19501 ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc attgctgaac
    19561 tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac catattacga
    19621 ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat actcgagaaa
    19681 ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt ggattaggtt
    19741 gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat gaagaacgag
    19801 tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg gctctattag
    19861 aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc tctaagttga
    19921 atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct aatcctttac
    19981 ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta acagaagtgc
    20041 cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa gaagtttttg
    20101 actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac ggggaaccag
    20161 tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta gaagataagt
    20221 ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta caatatggcg
    20281 aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat cttgtagctg
    20341 cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga agagggactc
    20401 cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt tttgaacgca
    20461 aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg gaagtaatct
    20521 tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg aaaaatgaac
    20581 aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga ttttcataaa
    20641 acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt tattaaaaga
    20701 cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta ttgattatca
    20761 ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc tgacagaagc
    20821 ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg tgcctgaaga
    20881 aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc aaaccgtacc
    20941 aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc gtttcttcgg
    21001 tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag caaatgaaat
    21061 tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg tagaagtgat
    21121 ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg ctcatgaaga
    21181 accagatttg aaggttctac cttcattccg tccagataaa gcactcaata ttgaaaaaga
    21241 aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga tcaccaccta
    21301 tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac atggatgtcg
    21361 tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa cagtattaga
    21421 agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa tcgatgctta
    21481 tcgtacagaa acactgatac atttgaccca cttctatcat tctcataatt ggacgatgca
    21541 gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat tagggcccga
    21601 taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa aattattgaa
    21661 ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga atccgaatga
    21721 ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg gaaaactgca
    21781 gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc aattgacaca
    21841 atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt caagaagttt
    21901 cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt taggcgaaat
    21961 ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag ttcaagcgat
    22021 tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta atagtaacag
    22081 taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt tttagagaag
    22141 aagacggacc aaatttgctc cgtcttcttc tcttgctgtt ctgcttcatt gcctataaac
    22201 ctttatgaaa gaagagataa catgcctact atccatattg ccggtgattc tactgcggca
    22261 gaaaaagatg aaaacaagcg tcccgagaca ggttggggag aaaaaatctc tgcttatttt
    22321 tcacccgaga tccagatcaa taaccaagca aagaatggac gcagcaccaa atcatttatc
    22381 caggaaggaa ggttagcccg tctagcttca cagtttcagc cgaatgattt cttatttgta
    22441 caatttggac ataacgacca gaaaatccaa gaagaaagag gtacgcttcc cttcggtagc
    22501 tatttaaaaa atctgcagat attttttgaa tgcgcaaaaa aagcgaacgt ccaaatggtc
    22561 ttactgacat ctgtgacgcg acgagattac ttagaaaacg gaacattgaa tccagatata
    22621 cttggagact atccaaaagc gatgcgtgca tttgctgaga aacaccatat tcctctttta
    22681 gatgtgttct caagatcaca agaattgttc cagaccttct caaaagaaga gacaaaaaaa
    22741 ttctacctgc atcttatacc tgatacttgt aaaaattacc cagaaggact aaaagacaat
    22801 acccacttta gtccagaagg cgctgataaa gttgcccgaa taattgttga attgataaaa
    22861 gaatcccgtc tgcctcttgc taattattta caaaaaggag tttgagatgt ttccattaaa
    22921 tttaggcatc cgtgcccacg atttagatgt tcgaaatcgt gaagaaatcg ttcaaaaagt
    22981 atcacagctt cacttgtccc atatccaatt tgcaccacaa aaagcatttc ctgaacaact
    23041 taaattaact gacatgacac ttgggagtgc tgcctatttt ggtgaatact tccaaaaaaa
    23101 caatatcgaa ctttccattt taggatgcta tatcaaccta tccagcaaaa atcaagaagt
    23161 tcgtgcacaa gcattggcaa ctttcaaaca gcaaattgct ctgtgctctg cctatcaagc
    23221 caagatgatt gctacggaaa ccggtagtgt ctctgtcggt tacacaaaag aaaattttac
    23281 tgaagaagct tatcagatcg ccagaaattc tatcattgaa ttagtcgctc atgcagaaaa
    23341 ttttggcgta actgttgctg ttgaagcagg gatcaatcat ccaatctata actatcagtt
    23401 agctaaacgt ttaatcgatg aagtagcttc accaaattta aaaatcattt tagattgtgc
    23461 gaatttgatg cataaagaaa atcatgctga tcaggaaaaa gtgatccgag gtgccttgga
    23521 caatttacat ggttatatta cagcgcttca tctcaaagac tatatcatgg aaaatggaaa
    23581 gatccgcatg gtacctgttg gcaagggctg tttagatttc cgcccggtac tgcattatat
    23641 caaatatgag cgtcctctaa tgtacgccac tcttgaagct actcctgaga tttatgttcc
    23701 tgaagcaata gaacacttgc agtttattta ccaagattgt taaggagaag attatggagg
    23761 aacgaataca gaaaagttat caaaaatatc atgcaaatgc ttttcagaca ttgctgcatc
    23821 tttatcaggg aaattattgg cgttttgcca tgtcaggact cttttttatt atcaaacatc
    23881 ttccatcttg gttaatgccg atcgctattg ccaatgtaat caatgcagct tcatcgaaag
    23941 attcttctca gttgcaggcc atttatttca acgctttttt gatggcttgt ctgattggtc
    24001 aaaatatatt gacgaattat tttcatgtaa aataccatag cacttcgatc cgtcaagtag
    24061 aagctggact aagagcgacc cttatcaaga aaatccaaga actatctatt ccttatcaaa
    24121 aagaattgca atctggacga atccagtcaa agatcatccg tgatgtagaa gctatccaaa
    24181 cgttgtcctc tcaagtattt gtcagcggat taaatatcgc tgtcaattta ttcgtagctt
    24241 taggtatcac gctttataaa agtcctgttg ttttcatgtt tttcttattg actgttcctc
    24301 tcgccgcatt cgttactctc tttttccgta aaaggatcag acagtccaac tcagacttca
    24361 ggcaagaaat ggaagagact tctgcaaaag tgatcgaagt gatcgaatta gcaccgattg
    24421 ctcgagctca tgggttagaa aaacacgaaa caactcgctt aaataagcat tttactcaaa
    24481 tctatgaaaa aggattccac ttagatatcc tgcaatctgt tttcggatca gtcagctggg
    24541 ctagttttca gatatttcag ctgatttgtt tgattttcac tgggatatta gctttacaag
    24601 ggcgcatcca acccggcgat gtcgtgatgt atcagacgta tttcacgacg atcgtcaatt
    24661 ccgtttctgg aatgatcacg ctgattccta cgatttcaaa aggcatggaa tctatcactt
    24721 ctattggcga actgttgatt gctggcgatg tagaaactta tcacggtaag aaaaaaatcc
    24781 aacatttgga tggaaatttt cgttttgagg atgtttcttt tctttatccg gactcactca
    24841 aaccgattat cgatcatttt tctctagagg tgaaagctgg agaaacaatt gcttttgttg
    24901 ggccatctgg ctctggaaaa tctacactcc ttaacttatt gattggattt atccagccga
    24961 cttcaggaaa aatttttcta gatgatcagc ctttggatga actggatatg cgaagtgtgc
    25021 gaatttattt agccgttgtt cctcaaacca ctttgttgtt ctccgcttcg atcaaagaga
    25081 acatcactta tggcttgaaa aacgtatcaa aagaacgttt agatgaagtc atcgaagctg
    25141 cgcaactttc ttcactgatt gaccagcttc ctgatggtct ggatacctta gtaggcgaac
    25201 atggcaacaa gctgtctggt ggccaaaagc aacgtatctc cattgcacga gcgttgatac
    25261 gtcagccaaa aatcattttg ctagatgaag caacctcagc tttagataac cagtcagaga
    25321 aaaaaatcca gcaagccctg aattacctga cagaaacacc cactactttt attgtggctc
    25381 atcggctttc aacgatcaaa gaggcagata aaatcgttgt gatcgatgaa ggaaaaatcg
    25441 ttgaaatagg tacttacgaa aaactgttag ctcaaaaagg ctatttctac cgcttgaaaa
    25501 atgaatgaca aaaaatagga gctatgacaa gacttttgtc gtagctccta tttctgaata
    25561 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcatcaa acatgagaag
    25621 ctgttttttg aaattgttcc ttgtaactca gagttaaacg gtaggctcac gacctcttgg
    25681 taaatggtgt tcaagagaca gcctatcggc aataagaagc aattttgtca tcgctcaagg
    25741 ccgaacgtct ctttcacacc ctctgttgtt tcataaaata gttttattcg ctgtaaaggt
    25801 cgcgaactgg tgtcatgatg atacggttca agtcattgat gattgtactg aagccttgct
    25861 ctttttgcat caagtcattg attacttctt ctgattggat tttcacagcc atttcttgtg
    25921 ctttcattgc atcttcgtcg ctgaactctt gtccactcat ttgtttttct tgtaattcga
    25981 tttgcatcgc ttggaagtct ttgaacagtt tgtgtgcttc ttcgtttgct tgtactttct
    26041 cgtacgcttc tttcaacgct ttgaattctg gtaattcacg gatctcacgt tcgatttgat
    26101 tcgcgctatc gtagatgttt gacatgttat gccctctttt cttttagtag ttactctcta
    26161 ttgtaacatg gggttattcg ttttcaaagg ggctactctc cttgatattt ccgccagagg
    26221 tctttagccg cttcaccaat tccctgaacg ccttctttca ccatatcgcc gaagtttttc
    26281 agcccttctt ttgcttgttc accatattga ttcaagttat cttgccaatt tccagactct
    26341 ccattagaat ttcctgtgtt tccaacttga tctgcagcta ccacttgtcc tcctgtggca
    26401 taggcatctg ctacactaaa tcctgttttt gaagtatacg gaaggatgcc ttctgccgct
    26461 gatttgaaga ctttcccgac aacatttcca cttgtccctt ccaaatagtg cagcttgctg
    26521 gattcttcgt aacccatcca tgtcgaaatc actacgtctg gcgtgtaacc gatcatccat
    26581 tggtcatttg cttttgtggc atcgaaatta gtttctgtgg ttcctgtttt ccctgctatc
    26641 gtataacctg caggattcgc agcagccgct gttccatttg aaaatgtacc aagaagcatg
    26701 ctggtcattt catcggctgt ttcctttgat atgacctgtt ttggtttttt gtttgtgtta
    26761 tcgacgatca ccgctccagt cgaatcaacg attttcgtga taagatgcgg cgtatacatc
    26821 tgaccatcat tggcaaatgc actgtaagca cccgccatga tcaatggaga tactcctttt
    26881 tccagtccac caagggctaa gccataatac ttatcagatt ttgctaaagg caggccgaat
    26941 tcttctgctt tatcatatcc tttttgtaaa ccgatctcat gcaaaagcca tacagtaggc
    27001 aaattcaaac tttgtgcaac agcttgatac atcggtactt ctccttggta tgtcccgtca
    27061 aaattatgcg catcgtaata actttgcggt tcgtctttca agatactgga aggcttgtat
    27121 ccagcttcca gtgctggtgt atatacgctt aatggtttga tcgttgatcc tggtgaacga
    27181 cgcatctgtg tagcaaagct gaatcctcta aagacatgat ctcctcttcg tcctactagt
    27241 gcttgtacac cacctgtatt cggatctaaa gctactgaag cagattggac catggcacca
    27301 tctgaagcat ttgctgggaa taatgcactg ttttgataag tagcttctaa ttgcttttga
    27361 taatcctgat ctaaagctgt atagatttta tagcctttat tcatgacatc ttcttcatca
    27421 agaccatact cgttaactgc ttcatcgatc actgcatcaa aataataagg ataacgataa
    27481 ccagaatctg aattatcgta tgtgtcatta agatacgcgc tgatatctgt ctggctttct
    27541 gcttgttctt gttcttttgt gatttttccg ttttcttcca ttacacttag tactgtgttt
    27601 ttccgatcat tcgcattttt tgggtgatcg atcggattat aaataccagg acctttcagc
    27661 attcctgcca aagtagcagc ctcagacagc gtaacattcg ttgcatctac tccaaaatac
    27721 tttctggaag cgtcttggac accccatacc ccgttgccaa aataggcatt gttaagatac
    27781 atggtcaaga tttcttcttt gctgtacttc ttttcgatct cgattgccaa aaataattcc
    27841 ttcgcttttc gatcaaacgt ttggtctaat gtcagatacg catttttggc aagctgctga
    27901 gtgatcgtac ttcctccgcc ccctgatgtt ccgccagaca agaccattcg gactgctgca
    27961 cgggcaatcc ctttgatatc aaacccgtga tgctcgtaaa aattcctatc ttctgtcgag
    28021 attactgcat cttggatata gggagaaatc gcgtttaatt ctacatatgt tcctttttgg
    28081 gcgtacagtg tgccagcttc ttcgtttttc ttatcataaa tgactgtcga ttggcttaat
    28141 cctgatttta acgtttcgac atttgcttgt tttgctaaaa taaagaggta gatacttgtc
    28201 actaagacac agatcaaacc aatcagcaaa atcaatttgt tcacatgata ttttttccag
    28261 atgcgctttc gccatcgatg gaattgtccg agatatggtc tgatccaacg ccaaaaactg
    28321 atcaaagcag cttttatctt ttctaaaatg ctttgaaaat ccatttgctt cttctcctgt
    28381 ctatttgatt caatcgtctt attttaccat agtgaaagag tgttttcttc ctgcttaagt
    28441 tgtagttcaa tatgtttttt tacgttagtg caagtagagt aacataagaa tgaacagaaa
    28501 aatgtaagtt ttttcttaaa tatgttttaa aaatggtaca attagtctgc gattgcactg
    28561 aaaggagtac acttgtgaaa atcacgatca ctttacctat gaaccatcct gaaacaacaa
    28621 ttcgcgactt gcttgaaaag gaatggttag ttcctagaaa ggttcgacac tttttgcgta
    28681 tacgtaaaaa cgtttggatc aatcaacaac ctgctttgtt ccatcatgaa gtaaagggca
    28741 atgaccagat cacattgtta ttcgaagaaa cagattataa ttatcaagaa gttcaattag
    28801 gagatgcgaa gcacatccgt gttttatatg aagatgagca tttgattata gtggataagc
    28861 ctgcaggaat gaagactcat ccaaatgaac cgacagaaaa cagtacctta ctcaatcatt
    28921 tagctgctta tctcgacact aaacagcaac gaccttacgt tgtccaccgc ttggacaaag
    28981 aaacaagcgg tgccgtttta tttgcaaaaa atccatttgt cttgccgata cttggaagaa
    29041 tgttggaaca aaaaatgatc tatcggagat atcaagccat cgtatgggga gaaattgcca
    29101 aagatatgac catcaccaat aaaattggac gagatcgcca tgatcggaga aaaagggtcg
    29161 tagatgataa aaatggtcag agcgccttga cacatgtcac tgtcaacaaa gtactttctg
    29221 aacaaacaca agtctattgt gttttggaaa ccggacgtac acaccagatc agagttcatt
    29281 tagctcataa gggacatccg atcgttggag accctctcta ccaaaataga acagctgaac
    29341 gactgatgct ccatgcatta gaactgcaca tgatccatcc atttactcaa gaaaaaatag
    29401 tcggtaaagc tttacctgga ttatgggaat agaaataaaa aagaacagag tcttatcagc
    29461 agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt gatgttcgtg
    29521 ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc aacgaacgat
    29581 atattcactt acttgaggtg tttccatata accacccatc aattcaacaa attgctgacg
    29641 gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa tcttttcagg
    29701 tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct gaatagccca
    29761 aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct caatagacgg
    29821 tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt cataatggtc
    29881 atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc ctgaaaaaat
    29941 ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt aaatacaact
    30001 attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat tgacatccgt
    30061 tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat cgaattgtgc
    30121 ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt tgtcgatacc
    30181 aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa aaaattcaaa
    30241 tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt ctaaaatttc
    30301 tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc cgccatacat
    30361 tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat tgggacaacc
    30421 gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt gtctttagtt
    30481 atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac tttatcacta
    30541 aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt taaatattgt
    30601 tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa aggaatctgt
    30661 gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag ttcaatgctg
    30721 taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata tccccaatcg
    30781 cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc tgttacttca
    30841 tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg aatattgaca
    30901 aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac atggtttggg
    30961 cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa ctttgtagga
    31021 aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc aaagtcgatt
    31081 tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg tttttctttg
    31141 gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt ttctaaacgc
    31201 tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc tatttccggt
    31261 gtatctacta atacttttac ttcagagttt tctttgttac tggataggat tggctgatca
    31321 cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc aaaaatatgc
    31381 agattattca actttcgttt gtttttgcga tcttccattg taatggtacc atttttttca
    31441 atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat cattgtttcc
    31501 ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga aaatgctggc
    31561 atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac acggaaacgg
    31621 tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc tggtatagtg
    31681 gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa ctcttgatat
    31741 agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt tatttctgct
    31801 tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct gtcttttggg
    31861 aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc aagatacttt
    31921 ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga atcgactgaa
    31981 caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc ataatctaat
    32041 tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg ttctgtaata
    32101 ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg agcagatgct
    32161 gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc ttctactgag
    32221 cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa attagaatga
    32281 ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga tatatctttt
    32341 tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga ggcatattgt
    32401 tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc tactggaatc
    32461 tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt ttgatcaggt
    32521 cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa aacttgatta
    32581 tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc agcttgtttc
    32641 attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc taacatgaca
    32701 ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac gttcgattcc
    32761 gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa ttttccttca
    32821 ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata gtcatctaaa
    32881 ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc aaatagctct
    32941 agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc catgtaccat
    33001 tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta cgatacctcc
    33061 tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat catatttgcc
    33121 caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc ttcgtgcata
    33181 aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt ttcagatttg
    33241 tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt ttctggcgta
    33301 tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt ttctttactc
    33361 aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata tcccaagtaa
    33421 ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt tcccattcca
    33481 tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc gtgattctga
    33541 atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc tttatcatcg
    33601 agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa catattggat
    33661 ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga ccatgtcatt
    33721 cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc ttttcttgtc
    33781 gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt gaacacgtcc
    33841 aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt tcctgtattt
    33901 ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc atattttctt
    33961 tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc gttagcttct
    34021 tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt tttgactaac
    34081 ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata aggacgaact
    34141 tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa aaaggtggtt
    34201 ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc tgtaaaattc
    34261 tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat aaatttctgc
    34321 accgattttg gtatatcttt ataagccact aataaaacct tctttctatt tgatatatct
    34381 ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata tctttttatt
    34441 tactttttca tccattatgg tattatttga atatgatata tcattaagag gtgtttatat
    34501 gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt taactggtga
    34561 attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct ataaagtaag
    34621 cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta tttatcgcgt
    34681 tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg cttctcctac
    34741 aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg ttggtgattt
    34801 tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga tgacgacgct
    34861 tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg gattgattta
    34921 ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat tgaaagaatt
    34981 ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg ttctttccga
    35041 taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac attcaaaaat
    35101 cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt accttggtta
    35161 ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg acaaaagcgc
    35221 gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt ttgtttgtga
    35281 aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt atcatatccc
    35341 tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg ttgacccgcc
    35401 actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag atgctttggt
    35461 cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt cattcatcaa
    35521 aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact agacatgcaa
    35581 cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc gttccttttg
    35641 ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg tttcatcctg
    35701 atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg atgggtgatt
    35761 tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc cttttccatg
    35821 ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg tcgatctgtc
    35881 aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga atcttatcta
    35941 ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc catcagctct
    36001 ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt tcagaagatc
    36061 ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt gatgagttag
    36121 ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca aaaaaacaaa
    36181 ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac ctggaactag
    36241 aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg cagtcctatt
    36301 ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt tatcataatt
    36361 tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt gggcaaaaaa
    36421 tccactatga tacgactagt cgatctataa aacctggtgt tctctataca aataatgggt
    36481 tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta gaaaaatacg
    36541 aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg ccaaaatggc
    36601 tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc attgcagatg
    36661 ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca atggaaaaag
    36721 gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa gattacttga
    36781 catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta gactatgctt
    36841 atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa atgacgaaga
    36901 aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca ggatttatgc
    36961 gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt tggggacaag
    37021 attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat ttttcaggac
    37081 tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag ctgtgcaatc
    37141 agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg agtgagatgg
    37201 ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac tatccttact
    37261 tgttcagtta tataggacaa cctgaaatgg cacagccact gcttaaacag ttgatgactc
    37321 agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga agtatggccg
    37381 cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc ggtgaatatg
    37441 ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga aaggaattga
    37501 cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc aaacggatgg
    37561 acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc ggcacgatct
    37621 cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat ttgccttttt
    37681 ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc aagtaataag
    37741 aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat taccgcagat
    37801 gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga cctgagtatt
    37861 agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct aataccgagg
    37921 gtcagctgat tgaacaccgt ttagtggaga ggtcgtgaaa aagctgttta gtttcgagta
    37981 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
    38041 agaaactggc ttttgaactc cgtttatcga gaggtcatga aaaagctgcc ttgcctcaag
    38101 taataagaac agcccaaaat tagatttcta gacaattttt tataagttat ttcattacat
    38161 tggctttttc ttttatgatt ctccactgtt ttctcggtac tctttagggg atctgccgac
    38221 gattttcttg aataatttag agaaatacgt ggtgttgttg tagccaatct cacttgctat
    38281 ctcattgatg ttttgattac tgtgcaaaag cagttgtttc gctttttcca tacgtaaatg
    38341 gttcaaataa gctgaaaaac tttttttcgt ttcttttttg aacagctgtc ctagatacat
    38401 gacatttaaa tgcaggtttt ctgacacttc tttgagtgta agtggttctt gataatgctg
    38461 gtgcaggatt acgattactt tttccacatt gtcactgtac tttttctgtc tctgatgctt
    38521 tttgatgaat gacagaagta gctgctgaag ttcttgcacc gtttttgcct gatggatttt
    38581 ttcgattcct tgtaaatatt cctcatcttc cagatcaatc aattcccgtt gaatctccat
    38641 aaacagtaaa aaactggaat atcgtatatc ttccggcatc atggctgcta actgaaactg
    38701 ttcaaaaaaa tcagtgatca ttttttgagc gacttccaat tgtttatttt gcagcacgcg
    38761 tctgaaactt gaaaaatcga tggtttgatc ttttgtttct gcttgtactg cgtaaaaaac
    38821 atgctcgtct ttatctccat aaaattgatg caactgtaga ttgtctttcg cttgttggaa
    38881 tgtttcaggg attttgtcca aagaatctgt ctcttccccg atactgatca gccattcctg
    38941 atcaggaaag ttttcttgaa caaaacaaca gaagccatca acttcatctg cctccaacaa
    39001 caacgtgatc aatacacggt ctccgtaatt tcgttgatag taaaagattt gttgccgttc
    39061 ttgcaaccat ctattgataa gagtatcatg agttcttgcc agttgaatca atactatcct
    39121 atgctgcttc tcacttattt gtcctattat ttcttcttca cttatttcat ccaattcttc
    39181 attcaaccat tgactgaata acagttcttg atagatttct tgttgatttt tttgttcatt
    39241 ttgctgatct aaccgtgttt tgattttctt gaggctttca accaattcga acttattcac
    39301 gggtttcatc aaataattca cggcgcctaa ttgcatacct cctttcaaat aatcgaattc
    39361 ttggtatcct gataaaatca taaattcaaa attatgacgt tctttttgag ccgcttcaat
    39421 aaattccagt ccatttattt ctggcattgt cacatccgtt actactaaat caatctgtcg
    39481 ctgttcaagt acagctagtc cttctttggc attctctgct gttgctacta tttgaaagcc
    39541 taaactttgc caatcaatta tcttttttaa cccattcaaa atcatgtatt catcatctac
    39601 caataacact tgatacacgc tcatccctcc ttgaatgtaa tagaaacggt cagtccacct
    39661 gtctcattat tctcaagcga catataataa ctgctgccga agctggcacg taaacgttca
    39721 ttcacgtttt gcagaccgat agaaccgtga agttccactt taggatgaga cagtttttct
    39781 tcaatctgtt ttagccgttt ttcagtcatc ccttttccat tatctttgat gatgatcctt
    39841 acccgtttgc cttcctgcaa gactttgaca cttagcgcat tatcgaatcg agtaaaatca
    39901 ataccgtgtt taaaataatt ttcaactaat ggctggatca caaatttagg tacttcgatc
    39961 ttttccaaat caggatcgat cataaaatga taggctactc gattaggata acgcatttga
    40021 tacaaataga catatttttc gcaaaagtcc agttcttctt ttaaagtgat cgttttctct
    40081 tgattcgtat tgtttcgaag caaagcggaa aacgcataca caacatctgc taattcttca
    40141 cttccttcgc tgattgcata cattcggata tattctaatg tattatataa aaagtgtgga
    40201 ttgatctgtg cttgaagcgc ccgcatatgt gcgtcttgct gtttgatttc taatttgtag
    40261 atgtcttcta caaactgatt gatgctatcc aacatttcgt tgataccgat tgaaagttcc
    40321 ttcagttcat attccgtgtt tgttatgtcg atccgcgtct ctaaattccc tgctgctgtt
    40381 tgatccatgg cctgcatgac gatagatact tgctgtgaat atcgtttgaa tgttttataa
    40441 agaaagataa gcagtatatt gtctaaaata atgataccaa tgatcagcgg ggcaagattt
    40501 tttcccgttt cgatttttac acttttttta tctagtacag ctaatatacg ataatcatca
    40561 gacagttctt ggtgttgaac aaaattgttt ttttccagat cgtcaatcgg caattgtgaa
    40621 tttttcttca gactctctgt gatgatcatt tcttgtcttt caaacgtttc tttagctatt
    40681 ttattatcat ggaatgtgta tagtcgattt gtcgttccag aaatcatgta taaagagagt
    40741 ccattgaatg acgttagatt ttctaaaatc atatctagtt ctttcttttg aaaaccaata
    40801 aaaatacttc ctgactcagc tcctccttgt gtgataggca acttgatata aaaggcgtct
    40861 agtaattttg gtgtcccctt ttttatttcc ccacctttgt tttctctaga agattcataa
    40921 tactccggta gttgattcaa cacgatatat atcgcactta gttcttcata atttgcgtag
    40981 agatctttga tttgatttgg gaaagaaact aattcatcgg tactgttttg ctgttcgtat
    41041 acatacatca gatactgatc tatgggtttt gttaagtagg tcgtcacatt ttcgatcttt
    41101 tcggaattcc ctacaagctg gtttttgatg atcctactca aggttgtttt atcattcagc
    41161 attctgcttg tgctttgaac ggcatcactt gtcatgtttt ctgcctgttt ttgattttgt
    41221 tcccaagtat ggatactaaa aataacggta gcagtcatcg ttacaccgat catgataacc
    41281 gcgtatttcc gcaacagacg atttagtaag gttccttttg aaaatagaat ccgaaactta
    41341 ttcataactt tccaactgtt tatctaattc ttcacgccaa tgtgttgtca tcgtaccatt
    41401 ccaaaccgtc aaaagaccaa tgacaccaaa caggatcaat cctttgaatt gccaagtcac
    41461 gcctaaaatt ataccgctac caataattat ttttagaaaa gacgaaaagc tggaaaaaac
    41521 cgaaataaaa cttagcttta gcagattagg aacagagatc gtatattcac tatctaaaat
    41581 catactgtat tgataagtag caaataggca gagcaaggca aaaagtaaaa taaaatcaat
    41641 agctaaaaaa atgagccctt tgatttgaac agataaatat aaattgtaac tcaataaagc
    41701 agtaccagat aaaaataaac taaatagcag attgcctctt aaaaaatttc ttttatatat
    41761 attccagcct ctttttattg tgatttcttt atactcgaat ccgtacaaat aaaatagttc
    41821 attgactgtt ttccatgccg cacctaatcc aaagatgatc ccgccggcaa atgtatatat
    41881 ccagaaatac aaggttagtt ttataatgac ccagatccgg ataaataact cttccaaaac
    41941 cttccctaac atatcttctc ctccaaacat taagtttata ctttctcatt atacaaaaaa
    42001 aggttgggca atcagcacct tgacctgatt attagagaaa caaacggaaa aatagttcct
    42061 catattttgt cgttttgtga gcgaataccg agggttagct gattgaacac cgtttaccaa
    42121 gagtttgtga aatagccgct tagtttcgag tagtaagaaa aattttgtga atatggcttt
    42181 ccacattttt acatatattt agcttaatac cgagaaactg gcttttgaac accgtttatt
    42241 cggaacaaga ggttgtgaca cgtcttttgt cacaacccct ttgtctttat tcattattat
    42301 tcttgtcgga actcatcata ttgtttttgc atttctttca ataccttatc atagccagcc
    42361 ttgtccaaag cgtctttcaa tttcggtaat gtttcatctg gatcaacagt ccctgtattc
    42421 aatccatcta aatattgatt catgacatta gaaatattac ttaattctgt tttgacactg
    42481 tcagtgttga agctgaatcc taaaatcggt gaagtttctg catcagcaat cgattgatca
    42541 cgtttggcaa tcatgtcatc cgtaatagac tcttgtgtat atagaatctt gttgtttcct
    42601 gtattccaag cagacatatg tgtatttggc tgataaccat ctagcaattt gattttatca
    42661 tctgaaccat cgactttttc ccatgcttct ccctcgatcc cccaaacaag cccattcaat
    42721 aattctggat cactgttaag cagccctaga agttctactg atttttcttt attttttgaa
    42781 gtatttgata cgacaaaatt tgccatttgt gcttgagctg tagattttag tggaacagta
    42841 attgcttttg agacaagttc ttgttttgct gcatttgtca aaattgtatc gccataatca
    42901 tacggtcctt gtgtttctcc acgcattagc caagtatttc cttctagtgg ataatcctta
    42961 ttgctcgttg cagcgtcact tggaatgagt ccttgtttgt accagctatg cattgtacgt
    43021 aacagtccga tatacgtttc attatcgtat tggttgatga ttttcgaatc atctccatcc
    43081 agatcaatag caaatggtaa cgtattccct aaaggataat caaagttccc ttggactttg
    43141 aagccttgtc caatggcaaa agctgccgtg tttggttctt tttcatggaa ttccttcaag
    43201 actttttctg catcagcgta agattggatt ccatcaattg aaagatcgta tttatctagt
    43261 aaagctttgt taaaagtaag tacttgttga gcaaagacat ttccattgac tgggaaagca
    43321 tataattttc cattgaccag atttccttta atatatgctt catctaaatc attataagct
    43381 ttttcagcgt acttaggcgc taattcagtc aaatcagcaa aggctccttt ttgggcattg
    43441 gaaacatagt tgttcgcaaa ggcgatatca taattttccc cggaagaaat gatgacgttc
    43501 attttctttt cgtaatcgcc ccagccaatg taattgatat tgacttttac accgatctct
    43561 ttttcgattc gtttgtttgc tacttccatc aattgatcga aattatctgg tttatctccg
    43621 atttgataca tctgcaaagt gtccgaattg ctagcttctg cctggtcttt agaaccagaa
    43681 cctccgcatg ctgctaatgt tcccaacaac aacgctgaaa cacctgtaaa ggctagtttc
    43741 ttccataact tcatttttct tcctcctaat tttatgattt atatttttgt ttctttattc
    43801 ttttactccg ccgattgtta gacctttgac aaaatgcttc tggaaaaatg gatagctgat
    43861 cgcaataggc aacgtcgaga tgactacgat cgccattctg gcagattctc ctggaactga
    43921 ggctaatccg cctgtcaatt ggcttcctgc tcctgaattt tgtgtcagat attgaatatt
    43981 actttggatt ttcatcaaaa gatactgtag tggaactagc ttatcatctt ggatgtatag
    44041 taaagcattg aaccagtcat tccagtaacc caatgcggca aataaactga ttgtcgcaat
    44101 acctggaaca gctaacggta aaacgatctg aacaaatatt cgcagttcgc tagctccatc
    44161 gatccgtgcc gattcaataa tcgaatcaga gaccgaacgt ttaaagaatg tgcgcatcac
    44221 aataatattg aagggactga cagccattgg caagatcaat gcccaaatcg tatcttttaa
    44281 ctgaagaaga tttgtcataa caagataatt ggccaccatc cctggtgaga acaacatcgt
    44341 aatcaaacaa aaaacggtaa aaaaacgacg gaatggaaag tttgaccgtg aaatcgcata
    44401 agcatataaa gaagtagccg tgctattgat cagtgtacct aaaacagtaa caagtacagt
    44461 gacgaaaagc gcttggaaaa ttttatcctg catctggtcg aacaagtacg tataaccaaa
    44521 tatactgaac ttttctggcc aaaaactgta cccgtgagtc gacaatatgg actcatcggt
    44581 aaaagagatg gcgataacaa atataaatgg caaaatacag gaaatagcaa agagtgcaat
    44641 cattatatta gagaaaaaat tagccgtctt attgaaagag cggacttgga ctctctccac
    44701 tttctttttt ttcatttttt tcctccttag aataatgcag aatcgctatc aaaacgtcgg
    44761 gcgatcgtat ttgtgatcat caacaagaca aagccgacaa ctgattggta aagtcctgca
    44821 gcagccgtca taccaaaatc tcctgtggat gtcaggccat tgtagatata cgtatccaaa
    44881 acttgagtca catcatataa tgcaccggaa ttcctaggaa tgttataaaa caggccaaaa
    44941 tcagctcgga aaatgtttcc tacggctaaa atcgttaaaa tcgtcattag tggaataagt
    45001 tgtggtatcg tcacatgttt gatctgctgc catttgctag ctccatcaac catagcagct
    45061 tcataataag tcggatcgat tcccataaca gatgcaaagt aaataatgct gttataacca
    45121 atccctttcc atgtaccaat gaacaccaag ataaacggcc aatatttcgg ttcattatac
    45181 caattgacag cttctcctcc atgtccagtg atccactgat tgaatattcc tttgtcagga
    45241 cttaaaaaag cgtaaacaaa gtaactgata atgacccaag atagaaaata aggtagtaag
    45301 gacatcgttt gataaacttt aacgagtcgt ttatttctca actcgctcat aatgattgca
    45361 aatacgactg agatcagcag attcaaaagg ataaatgcta cattatataa aattgtattt
    45421 ttagtgatga gaaatgcttg atccgaagaa aataaaaatt tgaaattttc ccaacccacc
    45481 caaggacttt ccctcaagct agccaagaac ccatctgctg aaatatgaaa atttttgaat
    45541 gctacgacat tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag catacccggt
    45601 acagccatca ggacaagtgc tttataacgc catagattca taaaaaaatg attgaacccc
    45661 tttcgttttt tcatctaaaa acccctttcg attcattcgc aacgtaatta tagcgctttc
    45721 aataatgaga ataaataaac aaagtatagg gaaaatgaaa caaagtatag gagaggttgt
    45781 gaaatcagcg ttttggcctg agtattagag aaaaaaacgg gaaaatagtt cctcatattt
    45841 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat cagaggttgt
    45901 gaattcagcg ttttggcctg agtattaggg agatcgtgtc cttactatct aactctgagt
    45961 cacaaggaac aatggggact gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac
    46021 ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaaa gccatccgaa
    46081 agtcagacgg acaagtctgc ttttcagatg gcgctacaac tactctgctt attcttttag
    46141 ctatccattc aattattttt ctctcacttg ttctagctct tctttggtca tgggtcgata
    46201 ttgcccttta ttcaaatctt taggcagcca aaattttccc atacggattc tttttaaata
    46261 taagacttct tttcctactg ctttgaccat tcgcttaact tggtgaaact ttccttcatg
    46321 caaaatcaga cggataattg aagtttctgt ctcttgatct acttgatcaa tttggagttt
    46381 tgccggcaat gtctgctcat cgccatcaat aatcagtcct tctgcaaata aacggatatc
    46441 ttcttctgtc attataccgc taacctcagc tagatattct ttctccacat gttttttagg
    46501 tgatagtaac tgatgggcaa acaccccgtc attcgttaaa atcaagaggc cctctgtgtc
    46561 tttatctaat cggccgactg gaaaaagatc ttcccgataa tcctggtctt ccaataaatc
    46621 gattacggtt tgatcatgat tatcctgagt agcagaaatg actcctgcag gtttatgcaa
    46681 aatgtagtaa aaatcttttt gatacgtaat gatttctcct aaataagtaa ccgcatcttc
    46741 gaattcattt acttgcgctt tatcgctttt caccacttgt ccattgactt cgatcgttcc
    46801 ttttttgatt aatcctttga cttctttgcg gctgccaagt cccatatctg ctaaaaactt
    46861 atctaaacgc atgcgtttcc cccatttcaa aagagcatca atctagctct tctgattcac
    46921 ttattttatt tttaatttat gtcgtaaacg cgctattcct ggtcctagta gtctttctgc
    46981 caagcggatt ttcaaagcta aataaccata cactaaagcc cctatacctg ctactacaag
    47041 aatcaacaat agagactgga atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt
    47101 tgtgatccac gaagccaaaa gcataatcaa ggtaagaata aagatcagca atgtcctgcg
    47161 ccagacaaaa cttggtttaa agcgggctac ttcatggatt ctctttaaga tcaaggtaca
    47221 agacacagca aatccgatca ttgtcgccaa taaagggccg tatacttcaa ataaacgaat
    47281 ggctgggtat tgaagaagga gtttcactag aaacccaata accaaataat taattgccgc
    47341 cttgttttca aacattcctt gcagcatgtt ggaaactagc atatatagtc ctaggaagag
    47401 tcctacaaaa cttgcttgga tcagtacatt acttcctaga gaatccggtg tataaaataa
    47461 tgtatttagc ggataggcaa gtaacataac accaaatgtc gccggaaaca taaaaaatga
    47521 aaacaactgt aaatttccgc ttgtcaattt tgccagacct actcgatctt tcaacgtaac
    47581 agcttctgta atcaacggta acccagtaga cgcgatcgat gttgccaatg caataactac
    47641 catcgtcaac ttatctggat tggcactaaa aatcgaaaaa agatccagta actgtgcatt
    47701 ggaatactcg gtggatgcag acatgatctt cataaaagtg aactgatcga ctaatttgaa
    47761 gatcgttacc cctgaaccca caataataaa cggaatcgct tctttaacgg tatctaacaa
    47821 caattccttc gttgcaatat ttattttgtt ttcgctgtag tgaataaacg ccgatgtata
    47881 cgcttgatgt ttcttcaaaa aatacaaaag gacaagaata cttgctagca taccgataaa
    47941 tgccgcgaaa gttgattgcg tgaccgccgt cacatagtca ccatccatga ctttcatgat
    48001 gatgaatgta gccaatagca tataaaaaat acgtgccacc tgttctacaa tctgcgacag
    48061 cgcataaggc atcatttctt gattgccttg caaataccct cgtatcacac tcatacaagg
    48121 aaaaaccaat acagcgatac tcaaagaacg catcgttgga atcagttctt ctccgccacc
    48181 agaagcatga gctagccaag gagaggcgat gtacatgaac aaagcaaaca acaggcctaa
    48241 cccgcccatc atctgtaatg cacggataaa taatcgtcgt gaagtgccat attcattcaa
    48301 tgaattatag cgtgcagttt gttttgcaat cgcagcaggt attcctgctg tagagatcag
    48361 tagaaacaat gcatatatat tgtatcccat attgaataat ccattggcag ccttagcatt
    48421 ttcgcccatc caagcatacc agggaatgat atagacagcg cctagcaatc ttgaaatgat
    48481 attactggcg gtcatccaag cagagcctcg tgccattttt tcttgcggtg ttaattgatc
    48541 taatgctggt tttctttgat tatccatcgg tcagattcca actttctaca aaacattact
    48601 atcattttaa ttggtaaaac agaaactggc aatcgatttt atataacttt tataagaaca
    48661 attgtttttt tgaattcggt ttcgtttttt tggtaaagta aagaaatata cagcctttta
    48721 tggctaacat ttataggaga tatcaatgac acaagaaata aaaaaactac ccgttatatt
    48781 tttaggtctt ttctttatca cagttggatt gaattggttc ttgctccctc acgatatagc
    48841 ttccgccgga gtgggcgcca tcggacattt agttgaaacg gccttttcta tccaacgctc
    48901 agttactgtt tggagcatca atcttatgat gctgcttcta gccgctgtcc tattaggaaa
    48961 atcagtattt tttaagactg tagcgggaag tctgcttttt cctgttgttt tagagattgt
    49021 tccaaaagtg gaaatgatct cctcacattt cttttcactg atttcaggaa gtcttttgtt
    49081 cagtcttggg gtttacacat tgtacacagt cggggcctca aatggcggta taacgatacc
    49141 gccgattatt ttccacaaat tttttcggct tccaatgccg caaggacttt tacttaccaa
    49201 tagtcttatc gtctttttga attattatgt ttttggattg ttggagacac tattcgttct
    49261 cctatctatt acagtcagtt cattgtttat gaaaatactc atccgcttgt cccctgtgtc
    49321 aagaaaattt gaataaagct ggattttgtc atagcggaaa caaacgatat attaaaaaga
    49381 gtgtgctata ataaaccaag aaattgaata aagcgaggga tcaatcaatg agcttctctt
    49441 tagaagacat ccgtcattta ctattaaaag aaaatctatt aaaagaattt gtttcaacaa
    49501 gaggctggca tcttgatgta ccagaaaaag ctgaatttct tcagttaagt tatgattcaa
    49561 gaaaagctga tgcttctact ttgtttttct gtaaaggaat gaatttcaaa gaagaatatt
    49621 tggattcagc aatcgaacag ggtattcaat attatgtatc agaacaacca tacgaaaatt
    49681 cagcagtagg aatcatcgta acggatatcc gtaaagcaat ggcgcttctt gctatggctt
    49741 tttacgacta tccgcaaaat aaattgaaag tgatcggatt cactggaaca aaaggtaaaa
    49801 caactgctgc gtactttaca aaagcaatct tggatcacac aacaaataaa aaaacagcat
    49861 tgctttctac tatgaataca acgttagatg gcaaaacata tttcaaatcc catttgacaa
    49921 cgcctgagtc attagattta tatcgtatga tgtcagaagc agttgaaaat ggtatgactc
    49981 atctagtgat ggaagtttct tcacaagctt acaaaacaca acgcgtatac ggcttgactt
    50041 tagatgtcgg aatcttcttg aatatctcgc ctgaccatat cagtccgatc gaacatccga
    50101 catttgatga ctatttctat tgcaagcgac aattgatctt gaattcaaaa acagttgttt
    50161 taaatcatga aagtgactat tttgatttac ttagggaaac agccgatcta ttccagattc
    50221 caacgatcac ttatggtcgt tcggaagaaa gcaattatca agtgatccgt tcagataaag
    50281 gtactcacgg ttttacttta tcttctcatg aagatcgctt agctatctgc gatactgctt
    50341 atgatatttt gctggctgga ggattcaacc aagaaaatgc agccagtgcg atcattgccg
    50401 cagcactaac aggtgcctcc agtcaagatg cacaagacgg attaaaagaa gctcgtgtac
    50461 caggccgaat ggatcaattg atccaatcca atggagcgca tgtctatgtg gactatgcac
    50521 ataattatct aagcttgaaa acattgctga aatttgccaa aaatgaacat ccagatggcc
    50581 gtgtaattgt tgtattaggc agcccaggaa acaaagcaat ctcccgtcga catgattttg
    50641 gaaaagtgct atcagaaaca gctgacatcg cctttttgac ggcagatgac ccagcatttg
    50701 aagatccgaa aaaaatcgcg gaagaaatca atgaagcaat tactaatcca gatttgatcg
    50761 ttcattatga aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa agcgctccgc
    50821 aagattcagt cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc aatggtgtag
    50881 atgaacctta tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa taatggtatt
    50941 tatatactag tggtttccca tttctaagaa aaaaagggac tgtgattttt tgtcacagtt
    51001 ccttttttct tagaaatcat tttaactttt cactagctaa ttctgctgcc ttttctcttt
    51061 cttcatttaa taatttatta tcatagaatt tgataaatgg gaaccagata atgaatgcga
    51121 tgattgcaca aatgactgct aacacagcag ctttccaatc accaccacta ccaacaaatg
    51181 ctccgatccc gactggtgaa ggccacggca tctgtgcaag catcggtttg acgatttcca
    51241 gccgaatggc aaaataagcg agtgtagcag aagccatcgg agctaagaaa aacggaaccg
    51301 ctaaataggg attgtagacg attggcatcc cgaaaatgat tggttcattg atattgaata
    51361 atcctggtac aagtgaggct tttcctaaca ctttcagctg atcagatttc gcgaggaatg
    51421 caataaagat gatcaatcct aaagtcgcac ctgatccacc aacggttaca taagaattgt
    51481 tgaattctcc tgccaacgga taatttgcgc cttgtgcgtt agctgccata ttagctaata
    51541 cgatcggggt caagaaagaa gtgatgatat tcgctccatg gatccctacg atccacaatg
    51601 catggatcaa gaaataaatc accatgacac caagccatgt attcgtcaac tgcgtaacaa
    51661 acccaaatgg gatagcaatc attttgaaga tatccgttcc tagtgcaact aataatccat
    51721 tgatgatcat caccacaaac gcaatgacaa atgttgggat cagagcagtg aaagaacgag
    51781 aaactcctgg cggaacaaca tctggcattt ttatgaccca atttctcttg acacacatac
    51841 aatacaattc gaccgcaata atcgacataa taatagctgt aaagattcct gaagttccta
    51901 aacgttcgac aaaagagccc atcctaatac catttactac agtttctgtc tcattgatag
    51961 aagcaatcaa gctgatttta ccatcagtca tgatcaattc agggatacac ataaagaatg
    52021 caaaaacaga tagtaatgcc cccgtgagcg gattaacatt taatttttgt tcatctcgtt
    52081 caattgacgt taattcataa gcaaacacaa tggcaaagta aatagataaa atccccatcg
    52141 tcattgtatt gacgatcatg tacaagtcac taactttgaa aaaagttgca ttaaaaaatc
    52201 cttctaaaaa tgggaacggt aatggcaaaa tatttaaaac caaaaacatc gagcctacaa
    52261 tcgtgaaagg aatagaagcc atccccgctg ccatgacagc ccgaacgatt ttaagttgcg
    52321 caattttccc cataggtccc atcaggtatt tctgcaaaaa atcaaacatt agttattctc
    52381 ctttcctaaa tataacgctt tttcatattg tttaatacgg atatactgtt ggtcttggtt
    52441 gagtgaaatt ttctttaaac gatactgtat aaatgcactg gcaaaaatac cgcttacaac
    52501 aagaatgaaa acaaataact gtcctccatt gttatttgcc ataaaaggat acaagaatgt
    52561 aaatcctgag gtaaacaaca ctggaatcac taataaatta aagacactct ggaacaaaaa
    52621 ataccacttt gtccatggaa cgttcgtttg gtgttttcga taaagcttta cttgttctcc
    52681 tactgcaggc aaaatagcta aaatcagaat catcggtaaa ctacttccta aacttttagc
    52741 tgatagaagc agaatcatcc attgcatgtt catgaaaaag aaaaaggcag tcaggtaacg
    52801 tacgattaga taacgattga aatacatgtt tttcaaacta agttgctttt tttctaattc
    52861 gattttcctt tttactgtat cctgcacaat gatccttcct ttctatttat ttttttgata
    52921 caaataattc atttcctgtg caacttccaa taatgtcatc gtcgtcatca gatgatcttg
    52981 tgcatggacc atgataattt ccatttctat ttttgtcccg ctggcatatt ctttcagaag
    53041 ttgagtttgt gactgatgcg catgaagaat ctcttcattt gcttgtcgca acttctcttc
    53101 tacttcttca aacatctctt tacgcatcga gcgaaatgct tcatgtatca gtgtccgtgc
    53161 atttccgcta tggaaaatga tatcaaaaga agtcacttgt agtgtttcac tgtttatttt
    53221 ttcagccatc tgttttcctc cttattcgat cagtttgata aatttactgc tgaaattctc
    53281 aaaaataggt tcagctaaga tttcctgctg tacttttagc tggtcaacta acctaacgat
    53341 tgctttagtc acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa ctaaaaaaac
    53401 aaaatgaatt tcttgatatt gatcccactg catcccagaa ggaatcaatg caacggctac
    53461 ctttgtttcg attccaactg gaacagcggg atgtgggact gccactgtat cactgaatat
    53521 gatttgtccc atattttcac gttgctggat ctgctttcgc atttctctta tatatccttc
    53581 ttcttcattt atgctcagct tttgcagcaa atcatgaaga atttcttcct ttttcggttc
    53641 gtacgtataa gtcttgaaaa atttttcaga caactgttcg gtgtaaatct gctttttttg
    53701 ctgtagagaa aatgctggta tcggtctatc tgaggaatga tagggtatag attctccaac
    53761 tacctttcgt attttacgaa catcatcttc attcaaaaaa atactgacat gtaaaactgg
    53821 aattttgaaa aacattgtcg aaagatcaat agaagaaata atcaggtcga ttccttttaa
    53881 agttgtttca ttgatctcat aatagccttt tacacttacc actgtaatat ttttaccgaa
    53941 ttcactgact acgcgattct ttaatagttg ggcactgccg taacccgttg cacagataat
    54001 caatgcatgt agtttatgcg cagctctttc tttttccaat gccgccatga gatgcaaagc
    54061 caaataggcc cattcatctt cattgatggt atagtttttc aaagccggca tgttgctgaa
    54121 atgccttttt actgcgtcaa agactttggg attttctttc tttatctcct cagtcaatgg
    54181 gttctcaatg cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat gatcgagtag
    54241 tccattgaaa agctggagat cctctactaa tgaaaaatga aattcctgag acaatctttt
    54301 cagaactttc gctaattcct ttgatagttc ctgatcattt tgattttctt tgtgattcga
    54361 cttagccatg agatgtaatg tcaaataagc aatttcttct tcgggaaacg atacacctgc
    54421 gattacctca atacgttgaa cgatccgctg cgccacttga tactctactc gcttagaaac
    54481 ttcttgttcg atgcctaatt ccttgatttc aaacccttct cggagccgtt tgatccccaa
    54541 agccaagtgt aaagtcagat tctgtatgat aaaatccgaa acttttaact tagcttctct
    54601 tgtttcatcg agaatgataa tgactagctc ttcaaaactg atgtctggga aaaaacggct
    54661 ttgccctaaa tattctttca atgaatttcc ataatggttt ccaaaaaaag tatccatgat
    54721 aaaatgccgt cgatttcttt cttccccttc gatccatgtc ccttgaccat gcttagaaac
    54781 gatttttagt gaatatggaa tcaatcgctc cttgatttct tgcatgtcct ttgtcaaact
    54841 ggaacgactg atataaagct cttctgccaa atcatccata aaaagaatgg catcttccaa
    54901 aagcaattgg ttcaatatat acttttgtcg atcttcggac tcataaaatc cttgcggttc
    54961 tctagcatat ccaggtacta tttcttgccg actcaaaaac aagtcaaatg tcagtctttg
    55021 taaaatatgc agttggtagc cataacctgg tttagcaatg atcttacctc cgtttttttc
    55081 tactaactct ttgatccgct gcaagtaatt acggattgtc cgatcagata ataacaattc
    55141 agaagctaaa cgttgactgg tgacatactc cttcttgtga tcaatcaaat atctcaacag
    55201 caatttttcc ttctgtttca ttatcatcca ctcctattcc atcatatgtt ctcttcgatc
    55261 acctctgcta acttctggat gcctgtaggg attgggacat acgcttggaa agggatacta
    55321 acaacaggct ttccaacaga ttctcctaat tttttgaatt tatccaaaaa catcgtcgtt
    55381 tgcgggctga tcagaaataa atcaaaatca ctattcttaa tcatattatc gccttctgta
    55441 gctgaaactg catctacgag tacctcttct cttttttgct taaagaaatc cgttgtcttt
    55501 gaggccatca tcgaggatga cattcctgcc gcacaaataa tcaatgcttt tttcataaat
    55561 caatcactcc ttttattttt ataaaccaag catatcttgt aagcgttttc tattcaaaat
    55621 atatatttcc gtagcattgc ggaaaaaatg tgtatcatat cttgaataat aatttctggc
    55681 accgtgatat actagtgata gaattttttc aggaggggat ttagtgagta tcacagtgaa
    55741 tattttgtat acaagtacaa atggacaagc cagaaaattt gcagaagaaa tggtcgaaag
    55801 aaacattgtc aaagcgattc gtgcagaaga aggaaacgag aaatatgact attactttcc
    55861 actagaagat ccagaatctc ttttactgat cgaccgttgg aaagatgaag aagcaatcga
    55921 aaagcatcac aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa agtataaatt
    55981 aaaaatgaaa gtcgaacgtt tcactgattt accgtaaaaa gatacacatg gaatgatagc
    56041 aatttatcat ccatgtgtat cttcactttt tccttatttt ttattcgatc tcatctaatg
    56101 attcaaaccg tgtttttctc ttatttttcg gataagtatt tcagtcaccc caatatacat
    56161 gattcctaaa cataccccaa ggatattctc aaaaatgcgg atcgttactg ctcctggaac
    56221 cccgaataaa ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg ttttatatct
    56281 ataggtagaa caaacaccta aagctagacc acctaatata ccgacccagt taaaaggaat
    56341 aaattggctg ataccaataa ataaaacaca tccaatcaat gagccaatga ttcggtctac
    56401 tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta gatacaatag aagaaaatgc
    56461 aaaccctgcc cacatcaatc gctgaaaagg gacatactct cctgcaacga tcaaaagact
    56521 aatgccaaat gcatagatga gcatccaaat tttttcctgc gaatacatgc ctttcaaaaa
    56581 aatctcttta aacaagcttt tgtctttgtt cttttctcga tgttttctgt aaaggagaac
    56641 tgaaaaccaa caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga aattcttgtt
    56701 caacgaatct ttaggtaacg aataaacaat aaagagatat gaaaagccgt acaaagaagc
    56761 attccccatt ttgggatcac tagcggttgc cattaatatg ctgcttagta aaataaaatg
    56821 aatcaacatg cccataactg accatttgat cagctggaca tacggtgcta ctaatagtga
    56881 gaacatgacg attgctaaac ctatgatcga atgggaaatc ttataaccaa agtcaacaaa
    56941 acgaatactc atcaacatac agaataaaac cacagcgtat ggtttatgct tcgattcaaa
    57001 taatgttgta attataatga taaaacaaat ggcaaacaat acgatgaata atgatcgtaa
    57061 aaacaatgaa caaatataca tgcacttttc ttttttagaa gctgcttctc gtattttttg
    57121 ttttaaaata aacggatcta gttgtagaga ctgataaaaa tccacctgtt gtcgtccttt
    57181 ctttttactt ttatttttta cagtcatcta tgacaggttg taaatactat ggaacatcat
    57241 atggcgttgt cattttcttg tcaactattt ttcctattca tgtttttata aaaaaacata
    57301 tcgttttctt gtatgattaa atcaccacaa aataaacaac tgagagaacg atatgcttta
    57361 tgaatggttc tatcaaaaaa attctgagat caatagacaa aaatttgatg attacagagg
    57421 tctcttacga aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg
    57481 ctccatgtgt ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg aaattaatgg
    57541 tggtgaaaaa caagaagaag gaaaccattg tccactttga ataatataat catatgccaa
    57601 tggttatacg cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg
    57661 ctcaaagtta ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga cataaaatca
    57721 cttctttact gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt taggtatctt
    57781 tgacaaccgt tattcgcaca ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa
    57841 gaattctgtc ttgagtgttg agggttgtga atttcgttct cacgtttccg ttgaggataa
    57901 actgagtttt atttccgcgg atggcgagac agaaaaatta atcgagaaaa tttacctaca
    57961 caaaaaattc ctcgattaac aaactatttc cttagctgca ctaatcctaa agaaatagaa
    58021 ttcttagtta cagacatgaa cgccacctac tttcagctca ccaaacatgt tctaccaaat
    58081 gcgaaattgg tgattgatcg attacttggt ttttccacgc ctttcaagga ggcgtaacct
    58141 ttattcacga gttggttgaa gctttccgag acaaaagacc cgacttgttt ttctccttat
    58201 tgacaaagag ccattaaaac tagagaaaaa attacaactg atcgctgatt atttgtagaa
    58261 agaaaatcaa agtattaccc aataaacttg agtcattagg caaaaaacac tgcgaaactc
    58321 aagaatgatt ttcgcagtgt ttttgtatat tttttattaa ctagtattac tacaaaggtt
    58381 tatttttcac ttctcaacgc agcacaaaat tgtctttact tatttactgg ttgggttcaa
    58441 ccttctctac tccgatacta atatttgttt gtatacccct tctaaaatcg gtagtttcca
    58501 ttcaattgat gggtactagt tgattttaaa aactatgctt attcaatttt ttattttagc
    58561 tcatatattt cgtttctttc taaattttat actgatgaaa acaagtagca cacccaatat
    58621 tgacaatata aactggctaa tgatggtttc tccagttttt ggaagatgct tatttccact
    58681 gcttgttgga ttgtttcctc ttatatctga gctatcagca acttttctat tctcttttgt
    58741 taaaaagatc gccacttctt gttctttttc gatcttgaaa gttactggtg tcgcatccaa
    58801 ttcatacccc gttggcgctt gagtttctac cagctgatat gctcctggtg ctaagccatc
    58861 tatcgccagt tttccatctg ctcctgtggt tagacctgac tgcagaacgg ttccattggc
    58921 atcttgtaac tcaaaaacag ctccttgcag gacttctccg ctttggtcat cgatctttgt
    58981 caacacgaca ccgcctggtg taagtcgatt ttctttcgtc aattctacca ccgctgtctg
    59041 acttctttcg atctcaaact cgattggtgt cgcatccaat tcataccccg ttggcgcttg
    59101 ggtttctacc agctgataca ctcctggtgc taagccgtca atcgccagtt ttccatctgc
    59161 tcctgtggtt agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc
    59221 tccttgcagg acttccccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt
    59281 aagtcggttt gctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc
    59341 gatcggtgtc gcatctaatt catacccc
//
LOCUS       DACIFS010000009        55736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.009, whole genome shotgun sequence.
ACCESSION   DACIFS010000009 DACIFS010000000
VERSION     DACIFS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.009"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..980)
                     /locus_tag="IYA83_000557"
     CDS             complement(<1..980)
                     /locus_tag="IYA83_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8586020.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTRE"
     gene            1463..1678
                     /locus_tag="IYA83_000558"
     CDS             1463..1678
                     /locus_tag="IYA83_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ8586021.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            complement(1919..2105)
                     /locus_tag="IYA83_000559"
                     /pseudo
     CDS             complement(1919..2105)
                     /locus_tag="IYA83_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252699.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            2539..3498
                     /locus_tag="IYA83_000560"
                     /pseudo
     CDS             2539..3498
                     /locus_tag="IYA83_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            4029..4439
                     /locus_tag="IYA83_000561"
     CDS             4029..4439
                     /locus_tag="IYA83_000561"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ8586022.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            4432..4887
                     /locus_tag="IYA83_000562"
     CDS             4432..4887
                     /locus_tag="IYA83_000562"
                     /inference="COORDINATES: protein motif:HMM:NF015769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586023.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            4904..5635
                     /locus_tag="IYA83_000563"
     CDS             4904..5635
                     /locus_tag="IYA83_000563"
                     /inference="COORDINATES: protein motif:HMM:NF015566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586024.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            5652..6464
                     /locus_tag="IYA83_000564"
     CDS             5652..6464
                     /locus_tag="IYA83_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ8586025.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(6680..7963)
                     /locus_tag="IYA83_000565"
                     /pseudo
     CDS             complement(6680..7963)
                     /locus_tag="IYA83_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8038..9449)
                     /locus_tag="IYA83_000566"
     CDS             complement(join(8038..8894,8894..9449))
                     /locus_tag="IYA83_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ8586026.1"
                     /translation="MAKYPFELKLKIVKEYLSGVGEYRHLANKYGISSKSQVSDWVNA
                     YREYGEEGLLKKHQNQKYSVQFKLNALELYQTSEMSYREVASTLEMNNPALIANWMRN
                     FREAGIEGLSKEKGRPLTLTRKKENKKENTTTEERERIKALEKQVRSLQIENAFLKEL
                     RKLRKQEAQQRRKNQSHESSPASEDPFKLVELLETLKFPKATYMYWQKRFDRPNPDQE
                     IEEKMLEIRKEHKDYGCLRLKKELENQGIHVNKKKIQRLIKKLGIEVKSYTRKSRRYN
                     SYHGKVGTVAKNRIHRRFYTNICHQKITTDTTEFKYYEVDTTGVVRQKKLYLDPFMDF
                     YNSEILSYRISEKPNALAIMEGLEEAIQMTNDCPFRRTFHSDQGWAYQMNAYKNKLKQ
                     HKIFQSMSRKGNCLDNSPMENFFGLLKQEIFHGEVYRSLDELKTKIDQYIYYYNHKRI
                     KKKLNWRSPVQFREAVKTAV"
     gene            complement(9538..9621)
                     /locus_tag="IYA83_000567"
                     /pseudo
     CDS             complement(9538..9621)
                     /locus_tag="IYA83_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10138..11388
                     /locus_tag="IYA83_000568"
                     /pseudo
     CDS             10138..11388
                     /locus_tag="IYA83_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(11962..12816)
                     /locus_tag="IYA83_000569"
     CDS             complement(11962..12816)
                     /locus_tag="IYA83_000569"
                     /inference="COORDINATES: protein motif:HMM:NF012515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586027.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(12833..13831)
                     /locus_tag="IYA83_000570"
     CDS             complement(12833..13831)
                     /locus_tag="IYA83_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8586028.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            complement(13866..14690)
                     /locus_tag="IYA83_000571"
     CDS             complement(13866..14690)
                     /locus_tag="IYA83_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ8586029.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            complement(14680..15462)
                     /locus_tag="IYA83_000572"
     CDS             complement(14680..15462)
                     /locus_tag="IYA83_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586030.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            complement(15486..15956)
                     /locus_tag="IYA83_000573"
     CDS             complement(15486..15956)
                     /locus_tag="IYA83_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586031.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            complement(15974..16396)
                     /locus_tag="IYA83_000574"
     CDS             complement(15974..16396)
                     /locus_tag="IYA83_000574"
                     /inference="COORDINATES: protein motif:HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose IIA subunit"
                     /protein_id="HAQ8586032.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            complement(16524..19247)
                     /locus_tag="IYA83_000575"
     CDS             complement(16524..19247)
                     /locus_tag="IYA83_000575"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012385.1,HMM:NF013070.1,HMM:NF015567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8586033.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            19437..20453
                     /locus_tag="IYA83_000576"
     CDS             19437..20453
                     /locus_tag="IYA83_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8586034.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            20561..21430
                     /locus_tag="IYA83_000577"
     CDS             20561..21430
                     /locus_tag="IYA83_000577"
                     /inference="COORDINATES: protein motif:HMM:NF012585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586035.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            21445..22149
                     /locus_tag="IYA83_000578"
     CDS             21445..22149
                     /locus_tag="IYA83_000578"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012610.1,HMM:NF019322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8586036.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            23370..23983
                     /locus_tag="IYA83_000579"
                     /pseudo
     CDS             23370..23983
                     /locus_tag="IYA83_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(24732..27881)
                     /locus_tag="IYA83_000580"
     CDS             complement(24732..27881)
                     /locus_tag="IYA83_000580"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ8586037.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(27894..29111)
                     /locus_tag="IYA83_000581"
     CDS             complement(27894..29111)
                     /locus_tag="IYA83_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ8586038.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(29101..30696)
                     /locus_tag="IYA83_000582"
     CDS             complement(29101..30696)
                     /locus_tag="IYA83_000582"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ8586039.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(31206..31988)
                     /locus_tag="IYA83_000583"
     CDS             complement(31206..31988)
                     /locus_tag="IYA83_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586040.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(32053..32889)
                     /locus_tag="IYA83_000584"
     CDS             complement(32053..32889)
                     /locus_tag="IYA83_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8586041.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(32907..34316)
                     /locus_tag="IYA83_000585"
     CDS             complement(32907..34316)
                     /locus_tag="IYA83_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8586042.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(34330..36153)
                     /locus_tag="IYA83_000586"
     CDS             complement(34330..36153)
                     /locus_tag="IYA83_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8586043.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(36996..37322)
                     /locus_tag="IYA83_000587"
     CDS             complement(36996..37322)
                     /locus_tag="IYA83_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586044.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(37368..37730)
                     /locus_tag="IYA83_000588"
     CDS             complement(37368..37730)
                     /locus_tag="IYA83_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ8586045.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(37767..37982)
                     /locus_tag="IYA83_000589"
     CDS             complement(37767..37982)
                     /locus_tag="IYA83_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586046.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            38109..38327
                     /locus_tag="IYA83_000590"
     CDS             38109..38327
                     /locus_tag="IYA83_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586047.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVEL"
     gene            complement(38521..40518)
                     /locus_tag="IYA83_000591"
     CDS             complement(38521..40518)
                     /locus_tag="IYA83_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586048.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(40616..40891)
                     /locus_tag="IYA83_000592"
     CDS             complement(40616..40891)
                     /locus_tag="IYA83_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586049.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            41123..41857
                     /locus_tag="IYA83_000593"
     CDS             41123..41857
                     /locus_tag="IYA83_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586050.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            41896..43038
                     /locus_tag="IYA83_000594"
     CDS             41896..43038
                     /locus_tag="IYA83_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8586051.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(43133..44524)
                     /gene="sufB"
                     /locus_tag="IYA83_000595"
     CDS             complement(43133..44524)
                     /gene="sufB"
                     /locus_tag="IYA83_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ8586052.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(44529..44999)
                     /locus_tag="IYA83_000596"
     CDS             complement(44529..44999)
                     /locus_tag="IYA83_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ8586053.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(44986..46221)
                     /locus_tag="IYA83_000597"
     CDS             complement(44986..46221)
                     /locus_tag="IYA83_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ8586054.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(46218..47504)
                     /gene="sufD"
                     /locus_tag="IYA83_000598"
     CDS             complement(46218..47504)
                     /gene="sufD"
                     /locus_tag="IYA83_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ8586055.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(47517..48290)
                     /gene="sufC"
                     /locus_tag="IYA83_000599"
     CDS             complement(47517..48290)
                     /gene="sufC"
                     /locus_tag="IYA83_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ8586056.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(48487..49335)
                     /locus_tag="IYA83_000600"
     CDS             complement(48487..49335)
                     /locus_tag="IYA83_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586057.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(49359..50045)
                     /locus_tag="IYA83_000601"
     CDS             complement(49359..50045)
                     /locus_tag="IYA83_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586058.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(50038..51072)
                     /locus_tag="IYA83_000602"
     CDS             complement(50038..51072)
                     /locus_tag="IYA83_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8586059.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(51467..51805)
                     /locus_tag="IYA83_000603"
     CDS             complement(51467..51805)
                     /locus_tag="IYA83_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ8586060.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(51807..52163)
                     /locus_tag="IYA83_000604"
     CDS             complement(51807..52163)
                     /locus_tag="IYA83_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ8586061.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(52169..53356)
                     /locus_tag="IYA83_000605"
     CDS             complement(52169..53356)
                     /locus_tag="IYA83_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ8586062.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(53547..54392)
                     /locus_tag="IYA83_000606"
     CDS             complement(53547..54392)
                     /locus_tag="IYA83_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8586063.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            54626..55591
                     /locus_tag="IYA83_000607"
     CDS             54626..55591
                     /locus_tag="IYA83_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ8586064.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        16879 a        11213 c         9257 g        18387 t
ORIGIN      
        1 tcttcccgtg ttcgactgta cacatccgca caactgattt taatgatatt cttcagctta
       61 ccaatattcc cactaaaggg atgagataac attagagaca aagcttcttt tgaaacaata
      121 atatcttttt gtaaaatatc cgcttcattt tgaaaaaata attgaatcag ctctaaccgt
      181 tctcgaatgg gacgatcttc aaaagttggc aacataacac tgatttgaat acgtcgatca
      241 aaggtgtcta ataaataatc ttcacttttt tccgtcgtgg cgaagataaa acgaacctta
      301 gcagaatgcc agtttacatt ttcaccgact ggacgataat ttccagtatc aataaaaaca
      361 aagagctttt cttgattttc tttcgacaaa cgatggacct catcaagaaa aagatagcca
      421 ttgttggctg ttgccaataa cccctctgta tccttctcag cccccgtaaa ggcccctttc
      481 ttgtatccaa acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacaattt
      541 aaaatagcaa atggtgcatc ttcttcaata atttgctcgt ggatagcgta ttcaaaaatt
      601 tttgtggcta aaaaactttt tccaaccccc gtggctccat ttattaatac actgagtccg
      661 ttaggcggat atttgacagc tgcaatacat ttttgaatga cctctctcag acttccgtca
      721 tgacccacca cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat
      781 tcatctaaag gcttccacaa aacaggcttt cccggtaatt tctcgacctt tttatcttcc
      841 actaatctcg tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt
      901 ttctcggcag tgacagaaga catattccct gttagtcgta tcagctcatc aaaaactcga
      961 tctttagcgc tctttttcat tttcccctcc agttcaacgt gtctagaagc ataataaact
     1021 atcggctaca atatgtctat tctatatttc tgttccaatt ttactttgaa acaaaaataa
     1081 tcgagcttaa actgatatta gttataaaac tatagttaga ttaccaatct tcctctcata
     1141 caataaactt ctctcacctg catccaacct acttatgtga aagaataact gccgattatt
     1201 tatctttttt acagttactt tcgaacataa aaaaatcacc ttcaaatact agaaaaacta
     1261 ctatttgatg gtggtttaga acaatttccg ttgattcagt atcgtctggt cccacatcct
     1321 ctgtctcatt aatcacaata gcatactcca aaggataaag ctggttgata tctcaatgcc
     1381 tttcacacct atcaattttg tcacacttat cttttcctag gttctattct atataatcta
     1441 gaaaaacaaa gaaggagaca ccatgaaaaa attcattatt ccttgtatat ttcttttctt
     1501 gagtattttg ttattttcaa cttccatcct agtcggttct tacctcgatt caagcggaat
     1561 gctgattgaa ccagcgttct tttgtgttcc gttgggctac ttaacattag gacttgcttt
     1621 ctttactgcc ttatacagtt tcgtcaaaca aagattattg aagaaatact cggcttgatt
     1681 tgcacgtaaa tcctctgttg aagaaaagtt agttttttca acaacgaacc ttcgagtatt
     1741 aagaatggat gttgatgtag acatcaaacg tctgcataaa cattcatttt attcctccat
     1801 taccaacaat tattcccaat cgccaaaaga agcttttttc ggaattacct tctccatttt
     1861 ttcacctgct ggttatattg tcttttgtaa taccgatagt cctcttcaat cattttcaat
     1921 atgcgaaaag aaccaatttt aacatggcac tcaaatcaca attcctaggg cggccaaaaa
     1981 gatacggctc gttgatttca agggtttcaa cgagttcatt gacaaaccca gcggtgctat
     2041 cttcttttgg aataagaagt tgtgatatct aaagtgagtt gattcatgtt ataatttgta
     2101 tgcatgattt tgatcctttc aagggttgaa tgatttagag tgcgagtatc ctctcgtact
     2161 cttttatttt acctaaaaag ctccagaaat gacgaatgac tcgtcatttc tagagctttt
     2221 ttcagagaat tatgtcacaa tctctttttg ttatattgat attctatatc tttttttatt
     2281 ggttttattc ccttgttcgt tatagactaa aatccttctt tcagcattat tcaaatcaaa
     2341 tggcaacata atagagaaat ttatagtatg tataatcatc tgaattcaat caaatcaacc
     2401 ggagtcatat acggagtcaa actatcccac tgagagttta ttaaacgcag attgtaaaat
     2461 taagctagac aactaaaaaa gtcatttgtg ctacactcaa aatagttccg accaaagaat
     2521 tataaggagt gaagacaaat gacctatacc catcttacaa caaatgaact cgtgatgata
     2581 gaagcctatt tcaatatgca tcaatctgtt accgtagtcg ctaagacctc aaatcgttcc
     2641 agacagacca tctacaatgt gtacaacttc ctgaaacaag gccaatctat ccatgactat
     2701 taccaacaat atcaaaaaaa taaaacacgg tgcggccgac gacccatttg tttgccagca
     2761 gaacaagatg cgtatatccg aaacaaggtg gcgcaaggat ggacacccga tgtgattatc
     2821 ggtcgtgctg agttttcaat ttcatgttct gttcgaaccc tttatcgcaa gtttaaaaca
     2881 aactaatttg attgctcgaa actacccatg aaaggaaaac ggaaacccaa cgggcataaa
     2941 gaacgtcgag ggaaacaagc cttcaaacgg aatatttcag aacgtctcac ggactatcct
     3001 gaatttcaac aggaatttgg acatttggaa ggcgacacta ttgtgggtgt ccatcataaa
     3061 agtgctgtca tcacgctggt tgagcgcttg tcaaaagtga ttattacttt aaaacccgaa
     3121 ggacggaaag cactagatat tgaaaaagtg acaactgact ggctacaaaa gcttccccga
     3181 aacttgttca aatccattac ctttgattgc ggaaaagaat tttcgaattg gaaaaacatg
     3241 agtaatgcta acgatatttc tatttatttt gcagatcctg gaacgctgtc acaacgggga
     3301 ttaaatgaac attctaatgg attgttacgt aaagacggac ttcctaatac aatggatttc
     3361 aatcaggttg accaaaaatt tgtggcggcg gttgcctcca aaagaaatca tataccaagg
     3421 aaatctttaa attatcgaac atcgttagaa gtatttttga gttacataag tgaggagcaa
     3481 ttgtctaact taatttgaca tttagaaaat aataaaccat atcccatcta ttcaaataat
     3541 attttaccga ttgaaagact tgctaatcaa agcagaatag tttatataga taaattattt
     3601 tctttaaact tatactcatt tttagatatt ttataagaat acttcatgtc ttcgccagta
     3661 atataaagaa catctgtctc tccatttgga cttgggttat ctattttggg tgtaacatac
     3721 tatacataca accgatacat aaaagatttg acatctggac aaaacacaat gctttacttc
     3781 atttttatag gctttgttta caccatcaca aaaatcacct cctaatttac aaattcatta
     3841 aacaaacaga aataaaaaat caccacgata gccgcgttca aaaaaattta caaaagagaa
     3901 ttttagttaa agtttttaga ttcgccccac taattaccaa aggtatttta tttattctta
     3961 atacgaaata atttattgac atttagatta ccaatggtat attatttata aataaatgga
     4021 ggtggattat gtacacaatt attcttgcaa gccacaaaaa tttagcgaaa ggaatgaaag
     4081 agacagtcga atatatcctt ggccctgcac cattcataaa agtaatgact gcatacacag
     4141 acgataacta tgatattgaa aaagatacga atattcttct cgatggaact gataaagtcc
     4201 ttgtcatcac tgatttactt ggtggtagcg taaataatca ttggatgaat tatgtttacg
     4261 aaaaaaaatt aacaaaaaaa attacagtaa ttgcaggtat gactttgtcc ttaattatgg
     4321 aattaagtat gaacattgaa gattataaat tgagagaaaa aatttccatg attattgctg
     4381 aaagccaaaa gagcattata aactgttcag aattaatgga ggtagaagat aatgattaaa
     4441 ctattaagaa ttgacgatcg cctgatccac ggtcaagttg caatggcttg gactaagtcc
     4501 gtatctgctg actacattat tgtagttgat gacgcctctg ctaatgacaa aatgaaacaa
     4561 atgattttat cattagcaaa acctgtatca acgggactag atattattac ttcagcagat
     4621 tttttaagta cctttgaaaa aaggaaaaat caaaatttga tgatagtcac tagttcccct
     4681 aaaagcgcat ttgaaattgt aaacgctatc ccaggggaag tcgattcaat taatatcggt
     4741 ggtttacggt acgccgaggg aaaagagaaa gttaacgagt atgtggctat cacatcagaa
     4801 gacaaaaact acttagcaaa attaaaggag tccggcttca aattaattat gcaggctact
     4861 ccaactggaa aagcgaagat ttattagaag gtggggaata agaatgattg aatgcatttt
     4921 aattggcata gttgggtatt taacgacaat agatgaaaga tattttgggg catctatgat
     4981 gaatcgaccg ataatagttg gacctgttgt tggacttatc ttaggggatc tccatcaagg
     5041 catcttgata ggagcagcat tagaagcaat gtttattggc attgtaacga ttggtgctgc
     5101 actaccacca gatgtaggtg tagctggtac tattgcaacg gctctagcaa taaaaagtgg
     5161 agcaggtgct gatgtagctg ttacattggc gatgcctttc gctgtattgg cacaaggatt
     5221 aaacatgttg gcttttacga ttaactcctt taccgtttca caaggtagaa aagcaattga
     5281 aaaccgcaaa ttatcatctt ttcagaagtg gcattttttt ccattaatcg ttcgcttacc
     5341 ttccgcagta cttacatttt tagtagtata ttttggtgcc aatgctacag aaagttttgt
     5401 aaatattatg ccagaaaaat taatgaccgg tttcaccgta gcttctggtt tactccccgc
     5461 tgttggattt gccatgctaa ttcaaggaat gattgacaaa aaatcagttc cttatttttt
     5521 cattggatac cttttagcgg catatttagg agtccccgta gttggtgtag ctgttgctgc
     5581 aattatggtt gctttattag ttatccaaac taaacgcaat cctgtaaata tttaaaggga
     5641 gagtgataat tatgggagat tctaaattaa ctaaaaaaga aataaacaag gttttctggc
     5701 gttcatttgc tttacaggga tccgagtcat acactgatat gcaaggaatg ggatatagtt
     5761 tttctatatt acctgcttta aacaaaatat atcaagggaa tgatgaaaaa tattatgaag
     5821 ctgtttaccg aaatacggaa ttttttaata caacgcctca tatttcaacc tttattatgg
     5881 gtatgtcatt agcacttgaa gaagaaaatg caaaaacaga tcaatttgaa actgaaacaa
     5941 tttcatcgat aaaagctgca ttgatggggc ctgtagctgg tattggtgat agtttttact
     6001 ggggaacatt tcgcgttatt gctgctggca tcggcttatc tttagctgcc caaggaaaca
     6061 ttttaggtcc aatattgtat ttttcaattt atacgctgtt acattttctt ggcagatatg
     6121 gctgcttacg tattggatat cggatgggag caaacgccat tgaaaaagca tttgagaaca
     6181 attaccttga aagtttctcg ttagcagcat ctatcgtagg tttaatgtca attggagcca
     6241 tgacctcatc tctggtacgc tttactattc ccgccgtatt taaatcaaaa ggtggagaaa
     6301 taaatgttca gcaaatgctc gatgatattt ttccaggaat tctctcattg gggctaacat
     6361 tctttgttta ctttctatta aagaaaaaag taaaggttct ttggattata cttgctatct
     6421 tcgtctttag tattgttgga gtttatattg gattattagg ttaattaatc cagaataaaa
     6481 ctagatcaga catcaactta aaccataaaa tacccaaaga taaatgtaaa tatgagtaaa
     6541 aagagcatgg tataaattat taggatctat aatttatggg tctgatgaat cagaattgat
     6601 tctgtagtga cctaaaaaag atagatgttt tcggagtgga gaaaattcac tccgtttttc
     6661 tttgcttttc ttgatgattt tagacagctt tgaatgctat tactctaaat tgcaccagac
     6721 tctgccagtt tagtttcctt ttcatacgct catggttgta gtaatgaata tagccatcaa
     6781 tggcttgctt caattcttca aagatacgat agtctcttcc ataataaatt tcctgcttta
     6841 atagactaag gaagttctca atgatcgaat tatctaagca ggtctcctta cgggacatac
     6901 tttggaagat cttatgttct ttcaacttat gactataagc tttcatttgg taggtccatc
     6961 ctttttggag tgaaaagttc gacgatagat acattccgtt gcgcagatag cttcctctaa
     7021 agcttccatg atagccaacg cacttggctt ttcagaaatg cgataaaata aaatttcact
     7081 gttgtacata tctaaaaatg gatcaagata cagcttcttt tcacgattgg caccgtattt
     7141 atccatttca aaatatttta gttctgtcgt atctgttgta accttttgat gacagttgct
     7201 tgtgtaaaag cgtcgatgaa ttcgattttt tgtaatcatg ccgattcttc cacgataagt
     7261 attatagcgg cgatattttc tcgtaaatga tcgtacttca atactcagct ttcgaatcaa
     7321 acgttgtact tttttcttgt taacatggaa cccacggtcc cacaattcct ttgtcatacg
     7381 taaatagcca taatctttat attgacttct tattttaagc atttcctctt cgatttcttg
     7441 atctgaattt cttcgatcaa atcgtctttg ccaatacata aaggttaatt caggaaattt
     7501 caaggtttca agaagctcaa ctaatttgaa tgatcctcgg aggttgtcga tgattcgtgc
     7561 gattgattcg ttcgtctttg ttagacttcc tgttttctaa gcttcctcaa ttcttttaaa
     7621 aaggtattct caatctgaag gaaacgcact tatttttcca attctttgat acgattatgc
     7681 tctttaggcg tcagtttgct tgtttcttcc ttttattctt tcttagacaa aatggaggag
     7741 gaccctttca tttttgagag cccgtgcaat ccttttgaac aaaaattttc atccaattga
     7801 caactaacgc aggttggttt atccctaaat gattcgccac ttcacgatag gatagctcac
     7861 ccgtttttta taactctatc gcatctatct tgaattgaac agaataactt cgcttatttc
     7921 gcttacgcaa gaggccttct tctccaaatt ctcgataagc atttgtagcg accccaaaaa
     7981 gttagacttt tatggagtgg agaaatccac tccttttttg tacgataaaa ttaactgtta
     8041 gacagctgtt tttacagctt ctcgaaactg aaccggactt cgccaattta atttcttttt
     8101 gatgcgttta tggttgtaat aatagatata ttggtcaatt tttgttttca attcatctaa
     8161 actccgatag acttctccat gaaaaatctc ttgctttaat aaaccaaaga agttttccat
     8221 aggtgagtta tctaaacaat tccctttacg agacatactc tggaagattt tatgctgctt
     8281 caatttattc ttataagcat tcatttgata ggcccatcct tgatctgaat ggaaggttct
     8341 acgaaatgga caatcatttg tcatctggat tgcttcttct aatccttcca ttatggctag
     8401 tgcgtttggt ttttcagaaa tccgataaga tagaatttca ctattgtaga aatccatgaa
     8461 aggatctaaa tacagctttt tctgacgaac gacacctgtg gtgtctactt cataatactt
     8521 aaactctgtg gtatcagttg taattttttg gtgacagata tttgtataaa accgacgatg
     8581 aatacgattt ttggcgacag tgccaacctt cccatgataa gaattgtacc gtcgagattt
     8641 tcgagtgtaa gactttactt cgatacctag tttttttatc agacgttgaa tcttcttttt
     8701 attgacatga atcccttgat tctctaattc tttttttagt cgcagacagc catagtcttt
     8761 atgctccttg cgaatttcaa gcattttttc ttcaatttct tggtctgggt ttggtctgtc
     8821 aaaccgtttc tgccaataca tataggttgc tttaggaaat ttcaatgttt caagaagctc
     8881 aactagtttg aatgatcctc ggaggctggc gatgattcgt gcgattgatt cttgcgtctt
     8941 tgctgggctt cctgttttcg cagcttcctc aactctttta aaaaggcatt ctcaatttga
     9001 agggatcgga cttgcttttc taatgccttg atacgctcgc gttcttcagt tgttgtattt
     9061 tcttttttgt tttctttttt tctagtcaaa gtaagtggtc gccctttctc ttttgagagc
     9121 ccctcaatac ctgcttctct gaaattacgc atccaattgg caataagcgc agggttgttc
     9181 atttctaaag tgctggcaac ttctcgatag gacatctcac ttgtttgata taactctagc
     9241 gcatttaact taaattgaac agaatatttc tgattttgat gctttttcaa tagaccttcc
     9301 tctccgtatt cacgataggc attcacccaa tcacttactt gagacttgct tgaaataccg
     9361 tatttgttgg caagatgccg atattcgccc acgccactaa gatattcttt aacgattttt
     9421 agttttaatt caaaaggata ttttgccata caaataacga cccccaaaag ttagattttt
     9481 tggtctaact tttgattgat ccatccgtgt accttttttt gatctcactc cttgtatttt
     9541 tgctaaatat cctaatccac cttctccttc aagatactct tgaacgattg ttgacttgaa
     9601 ttcaaagcta tattttccca taaaaatacc atcccccctg aagttagatt ttggtctaac
     9661 tttttagggt ttggacatca ccagtcccat tttcattcac gtaatgcaag aaactgactg
     9721 aagtcattaa acaaaaatct gagctagaat gaagttcatt ctgactcaga tttgattttg
     9781 cacaactcat cagtcttcgt aaaatatatt ttgcctaata agtttctttt aaagtatcta
     9841 taaaacacaa atttttttga ataacatatt ccttccggaa tttatttcgt tattgtgtga
     9901 tttctcagag tacaatggat gtatggaaag gattcaacga gaatttgcta atcagtcctg
     9961 tagctttgtc taccttcata agtctgttat ccaaagcgct gtgtttacta tcaactcatt
    10021 agctctttta ttaccgtatt ctattaggcg aggtcgtgac cacacagtga gtttctgtgg
    10081 aatcccacag tatcgaagca ttttctagaa tttatatcga aaataggttt ttattccatg
    10141 aaattatttg ttggtaatga cgttagttct gaaaaattag acgtttgctt cctaacagac
    10201 gacgatcagc tatctatatt gactgaaaat tctgttgcta atgacattga aagggctcca
    10261 ttgacacgag aactgattct tgtgttcaat gaaaacaatc acttcggtca aatcgttatt
    10321 gagatggaat taaacgtcca tgtacagctt tcatccttcg atgtttttcc atgaagatgc
    10381 agagctaaaa aaactcaatg cacttgtgag gattgaaaat ccttttcgga tgaaacaatt
    10441 cagtcgcatg tttgaggaag atacgactga tcgaaacaac gcgttaagaa ttgctgattt
    10501 attgcgtatt caacggttca caacttctcc tatcaaagaa gagaagtacg tggctttaca
    10561 gcgtttaaca cgcactcgct atcgactcat taagcaactt cactcgtaca aaacaacact
    10621 ttctggaaaa tctaacgtac aaatgcaata cgcttactcg ataaatgcgc gatgaatcga
    10681 caagtctcta tagtgccact gtcattactc ttgtgacaga agacttcacg ctagatgaat
    10741 tggctgtgct tcctttggaa gctttctgtg acttactaaa agaaaaagga aatgtcgctt
    10801 taaagatcca gaaaaaaatg ccaaatcgat tcaacgtgca attgccgtga gttatcggtt
    10861 agatagtctt gcataagaat ccatcaatgt cgttctaaat gtttttgtat gtgaaattcg
    10921 tgcgcttgaa aagaacatca aagacttgga taaagcaacc gagcaatttg ttgtcgttat
    10981 tccagaatat caatatttga ctagtgttcc aggtattgga aaggtacacg ccgctggatt
    11041 aatcgctgaa atcggtcaaa ttgaacgatt tgaagatcaa acaaaattgg caaaatacgc
    11101 aggcttatgt tggaaaggca aacaatctgt taactatcgg tcacaaaata cacctctgac
    11161 aaaacaaagg agtcgctatt ttagatacta cttagttgaa gcttccaact ctgtaaaaaa
    11221 atatcttcct caatataaag ctttttatca aagcaaatgt aaagaggtcc caaaaataaa
    11281 cacaaacgag cacacgtctt aaccgcaaga aaatttacgc gtctggtaga tacgctacta
    11341 cgtaaccatc aactctacac gccaccaagg aacgtgatag ataaataaca ttctgttatt
    11401 gacgcaaacc cttgatatac cccgaaaaaa tttgattttg gccggggcta gttaagtacg
    11461 cttcaaaata ggttacacaa taaaaatatt ctcaatctcc aacttgtctt attaccatta
    11521 gacttattga caaagagaca attaccatgc tcttttttac tatttagtct actatatatt
    11581 ggtttagaca gccttccaag tcttatttca tcacgcagcc taccaagtcc tttaaaaata
    11641 aattgaagct cgcaataatt tcagcaaata atcaacagca atatttttgg gtcgtacata
    11701 acatgaagtg cttgtaccta ctagtctcgt taagtaacag aagattatct ttgcttgact
    11761 aatcaataaa ttaagtaaat aacaatataa tattatcgag atatttattc atactatcaa
    11821 tgacgcttta acaacgatat gctccctcat gcaattagcc agtgttattt tcatcatagc
    11881 ctcttgcctt ttcacataca aaaatatagt tatgatgagt ctactataca aattccagca
    11941 ataaaagccc aaagcacaaa gctaagggtt aataggtgta gcatgaccaa aggagccatc
    12001 aatcatcaat gtttcatatg caaggtttgc acctttgtga aaacaatcaa taacatgttc
    12061 tatcattgat ttttcacgtt tagataagac aaaattactt gcaaaagctg caaagtatgc
    12121 atcacctgcg cccattgtat catgaggtga ctcaactttg cgaatgaatc cttcataagt
    12181 cttattctct cgagtgtcaa caaaaacagg gctcttacta ccacgcgtaa aaagaagata
    12241 cggaacctca aagtccttgc attcactaat taagcgatta atttcctctt gatcatctgt
    12301 agcgactgaa aactgtgcaa tataaattga cggacaaaca tctgacaagt attcatgcgt
    12361 tctatattta tctaagtcag aaaaatcaaa agaaacttta acatcatatt tttttaattc
    12421 tttaattttt gtttctgttc gactatggca actagtatga actaatgaac tgttcttcat
    12481 caaatcaata agttctgctg agagagcagg tgtcaatcgg gagccacgca cgccacctat
    12541 aaactcccta tctccatctt taatagttac atgtgcttgc tcagtaggct cgtcaataac
    12601 ttccgagaaa gagatatcaa caccttcaga actgatggcg tgttgtaata acacgccatc
    12661 aggatcagat gatagactac cgacatagta tgctttaaca ccactctttt ttgcattcac
    12721 agcaaaatta actgcatttc cccctggaaa tttttgattc aaattctcat agtaatcaac
    12781 aacattatcg ccaaatccaa tcacatacgc tgtcataatt aatcacctac ttttagtatt
    12841 caactactcg gtaataacga cgtttatcca aagagtggtt ccgaatatct tcaagatgaa
    12901 cactgacgcg acctagggct gcccacataa ctggagcaca aaagtacttt cgaaattcgt
    12961 cactaatacc tggtagctca taatccttag tatcaaaaac attaaattta tctgtatatt
    13021 ggaccgcaaa tttttcaact cgagcatcta aaagccgtgt ttccccctca gttttcaaaa
    13081 gtgttacaca aactcctttt tcgactaact cgagggtacc atggaaaaac tctggtgatg
    13141 agacagactt tgtacgcaac cattgagatt cctcaagcac acacattgaa taagagtagg
    13201 tgattcccca taaattcccg ctaccaatcc aaatttgata aggatcttct ttaaattctt
    13261 cggcatactt cttccctttt tcatcagcct gttctctaac tgaattcaga gccgttggta
    13321 agtttttcag ttcatcagca aaacgagtgt attcaggaaa ttccccattc aaattcatga
    13381 tagacataat taagaaataa agtggcattt cttgagggcg gccgtctagg taattgattg
    13441 cataagttga tagagcctct aacggtgtat cttctttact tacaatacca acggttggca
    13501 cgtttcgttc ttttaaatat tcagctgcct taatagtttc tttcgtatct ccagattttg
    13561 agtataagat tactaaagat ttttcattca actgtttatg tcccattaag acaagctcag
    13621 ctgcagtcat agagtaaaca ggaatacttg attcagtcat caatacatac tcaaatggtt
    13681 gcattacggc taccgatcca ccagaagcag taaagaaaat attactaatt ccagaatcat
    13741 aagcttgctt tgcaattttt tctacatctt cacgaatacc gtaaatccgt ttgccttttt
    13801 ttttcatttc ttttgcatta tagtttaaca ttagttctct ccttacaatt atttagatta
    13861 ttcaattaag ttgtcaaaaa tccgaagaag gctgctatga taccaaagat aaagattccg
    13921 aataacacgt aagttgtttt aaagcctttt ttaagcatcc agtaaatcag aaatacacat
    13981 aatactgaca gcagatttgg taaaatacca tcaatgatac tttgaatttt tgttgcttca
    14041 tcaccttgtc caatagaacc agctactttg attgatacca tacttccaga catgccccca
    14101 attaccataa gacccaaaat tgaggcagca tgagtcaacg ttttcatgac gccagaggat
    14161 tcaatgttct ctaatagctt tgtaccaaag ttatatccct gcataactcc aaagtaacgg
    14221 agaagaaaat tagggacatt gaagattagt aaaaataaaa atggacccaa aatattgcct
    14281 tcttttgcta atgaagcacc tacaccgata ccgataacta ataaggtacc ccaaaacatt
    14341 gaatcaccaa taccagctaa tggtcccatc aatgcagttt taatagaagt gattgtagaa
    14401 gtatcaaagt catcaggatt tttggcattt tcttcttcca tagaagccac aattcccatt
    14461 atgaatgttg ataatggcgg agctgtgtta aaaaactcta gatgtctttg agctgctttt
    14521 tgcttttctt cttcattgcc actataaatt ttttctaacg ctggcatcat agcaaaagca
    14581 aagcccatat gttgttggcg ctcaaaattc catgatgctt ctaatgcaca tgaccgaata
    14641 caaatttttg ttaaatcatc tttagttaaa gctttcccac tagaagtcat cttcaaccat
    14701 ctcctcccct cttgaaccca ttgcagcttt ggtttccatg tttactataa tataagctag
    14761 acatgcgccg aagattgcaa tccccagaat cggcacatgt aagtagctaa agagtgcaaa
    14821 accaattacg aaatatacaa agacatcttt cttaagaagc atttgcaata aaatagcaaa
    14881 acctagtgct ggcataatgc ctgcagcaat gttaatccca tcttggacaa aagttggaat
    14941 agcatttaag attcgttcca taacaggact tcctaagtaa aatgagaatc cgacaaataa
    15001 agcccgtggt aaacttttta ctaagataaa acctaaaaca tgcattttcg acacattttt
    15061 tagttcgccc ttttcagcat atctatctgc ctttctagca atcattggta gaacaagcaa
    15121 tgttagtaga ttatcaacta ataagaatag cgcagcgatt gggaatgcca aggtcaacgc
    15181 aaattctgct cccttgccag ttgcaatagc aaacgcagtc cctaaaatcc caccagatac
    15241 cgtatcaggc ggacgtgccg ctccgattgt tattgctccg ataaatgcta actccagtgt
    15301 tgcccccatc ataatcccca tcttaagatc gcccataatt aaaccaacaa agacggaaga
    15361 tacgattggt cttgataagt tattcgtacc tgtcatccag tcaattacga tgaacgcagt
    15421 caagagacca agcaaaattg cttgtactaa gagactatcc atgaaattac ccccttattt
    15481 ttttattaca aagatttttt taaatctatt gccttatctg ttggaacttg ttgagcgaac
    15541 acttcaatgc cctctgagac cattccttta agttctgctc gttcctcttc tgttacaaaa
    15601 attgtatgcg tcaaagcgct actgccacta gtttccttaa cactaccgat atttacagat
    15661 ttaatttgtt caaccccatt tgccaaccgt ttagcatcgg aaatggtttt ggtaactaca
    15721 aatagtttgt atttatctgt cttcccacta ttaattgcat caatcgaatc ttgaattgat
    15781 ttgataacaa ccttcacgtt atgtggtgcg gctaatttca aagttgtctt ttgaattgga
    15841 tcattcaaaa tttcatcgtt cgctactaaa atgcaatccg cactgattgc gttcgtccaa
    15901 gttaaagcaa cttgaccgtg tatcaaacga tgatcgatcc tcactaattt aatcattctc
    15961 ttccctcctt tatttaaaag tcatcaaccg tttgctcata atctagctga ttacaaaact
    16021 taatcgaacc agaagatgct tgaattgcat gatcaataat ttcttcaatc tgatcagatg
    16081 aaactaattg gaattttaaa tataaatcca ctaaaagact taaattcatt ccagaaatca
    16141 aatagacttt agaaagatct ggcaaccgtc ttagccactc attattaatg cttccaccaa
    16201 aaatgtcggt tacaacaatc gtctcatttc ccatagattc ctttagataa tcatcgatga
    16261 tcatttctac gtcattattt ccatcgacat aggcacatcg aaaatcaata ttatccaaat
    16321 ctcctacgat aatactcaaa gtttcaatca ttccttttgc tagaggaccg tggctcatac
    16381 ataatatttg tctcataccc gaactccttt cgctttgtta ttagcaaata ctgtgccaat
    16441 tactgtcacc acttaaatga aagggcgtac aaaaaataaa gtgacactat ttccatcgct
    16501 gtgaatagtg tcactttatt ttatcaataa cttacaatga tgttgcacac atatgcaatc
    16561 tcttccatag gaatcttaat attataaagt ttctcaatct tactaagtgc cttttgaata
    16621 gaagcaacct cagtttcgta tcgcttaaga aactgatcta ttgctggata actacttact
    16681 ccctgattac gaattagtcg ctcaatcata cagctgatat gaacataaag attaattttg
    16741 gacaatgatg ataacgtttt ccccaccctc atttgataat cttttaaact ttcctcaatt
    16801 tgattgacaa ctttttcggc atctaaaata gtaagactgt caatcaagcg gtttaatgag
    16861 ctatttttaa caatattttg attaattgcc tcagcaatct cttttccata gactctcaaa
    16921 agaactttat agagtttgcc aatttcacca tcaaataatt cttcaagaga aatataggga
    16981 atgttcgaca cacgcggatc atctgttccg ataatggcaa tgatatcctt tgttttaatg
    17041 agaaactcta attgctgagt atctctaaga gtttcatttt cgtatggcag aaattcaatg
    17101 cccaagtctt tcgacaaatt attttccatc atttctttaa tttttatgga ggtacccaaa
    17161 ccagtattgc aggaaacaac gattgtacgt tttctaattt tgatgggata tagaacttgc
    17221 ttttcaaact gagaatacgg caattttttt acaacctttt ggatatcact cgtactttga
    17281 atcagttgtg cggtttcaat agccatttcg gttgtaacat tattaataat cattgttgga
    17341 acttcgatta cttgttcaat ttcagtctta atttgagcta aacttcccat atcaacgaaa
    17401 atcactagtc catctttagg ctttcgatac tctatatagt gaagtaatct gtcaattact
    17461 tcactaaagc tgatatctag aggcatatca atagaatcta tgacaaactg attaaccaaa
    17521 cgattggcaa cattagcaat actactagct gtcgcatatc catgggctaa aataattgcg
    17581 ttaatgtctt tcgcttcatt tccggtctca ctaataacac acaaactgat atacagttta
    17641 tcccaaatag tcaatgttgt atccagggcg ttctcgaaga tcagtaagaa tacatttaaa
    17701 tccgtcaaat agttacactt taggttttca tataactttt caatttcatt cgctgaagca
    17761 ttctctttcc cataccccca ttgatcccta atagcgagat aggtagctat agcttggacc
    17821 gtattgccag aaaggtgaac aaaaagactg ggagagattt tttgaacaat attttggatt
    17881 tccggtaaat aatacttaac taagacttct gaattttttt ggtaaataaa acgatcatta
    17941 aattctgcca tttggtcctt cacaaaacgt cgaaactctt cactatttct attcacatca
    18001 tgacactctg aataatattt agaaacacga tgaatcaaac ctcgtaaatt tgtagttaca
    18061 gttgaagtct cgtcacaatc atgaacatag ttaccattac taaagatata gaaccgttgg
    18121 ctcgcaattt ggttttttaa ctcaatattt tctgttagca agtacctgct aggtaaatca
    18181 gaaatatgaa tttcgatata ttttttattg cttccactaa atagtttatt cgcaatagtt
    18241 tgttttatta gtccatccag ctcaccaatg tttccataca aatctaagcg aatcaggaaa
    18301 ctaagggcgg cttcagaaac taatatttct ttattgatca gagtagactc tttctctaat
    18361 aaatggaaga caatctcttt tcgttcgcta atttctctat cttggtaatt aggcaaagta
    18421 caaacaatcg gaattcttcg tcgaaaagtt tctagaaaaa aattttcttc ctgttccgtt
    18481 gtcgcaaaaa tatatctaac atgtgataaa cgccaaggac cactatctcc aactcttcga
    18541 aaaacgcctt tatccatatg aataaaaagt ttctcttgtc cttctggtgg taaacgatga
    18601 atttcgtcaa gaaagacgat tccaccgtct gctgcttcta ttataccaat atggtcagaa
    18661 tcggcgccgg taaacgatcc cttcatatgg ccaaacaact gacttgttaa taactctgga
    18721 ttattaaaat actgagcgca gtttaactcg atgaacggag cattctcatg aataatacca
    18781 gtttgtcggc aatactcagc tagtttttcc actaagtaac tcttcccaac tccggacgat
    18841 ccaaaataca ttattggtaa tccacctgga tatttcattg ctgatatccc ttgttgaacc
    18901 gcttctttta aactttgttt tgcaccaatt aaatcatcaa aaaaatgatc tgcttttttc
    18961 tttgaaattt tttctctaaa cgaatcaatt gaagaaaact caaaactatc taatgaatgt
    19021 gatatatacc gttcaattgt atggactgga aaaaaaagaa caggacgacc attgattttc
    19081 acaacttttt tctgttccat tagttcgttt agatgcttac tagccgtatt tctttttatt
    19141 tgaaattttt gggcaatata ttgcgcgtta cattcttctg gaaaatattg tggaaaatca
    19201 tagctgttaa gtagatcatt taattctttt aaaattttcg ttttcaaatg aacccacctc
    19261 ctttacttaa taataaatga ttttcactat aaaattcaat attctttata aacatctttc
    19321 cactttaaca atacctatgg tatttttcct aaaaaatatt tgacactttt tattacctgt
    19381 ggtattttat ttatgcaaac aaaatattac cattggtaat caaaggagag atttttatgt
    19441 ttaattttga tcaagaaaga tttttaaaga ctcaagctgg tgccgttgca acaaatgaac
    19501 aaatttccaa tattttagaa tccatcgcat ctgacttaga agaaatttac tttattgggt
    19561 ctggaggagt tggtattctt tttgattcaa caatcgaatt cgcgagaact cgtagcactt
    19621 tcccttttca tcgtgttatc gctgccgaat ttatgattca tcccaataaa acattttcta
    19681 acaaatcttt ggttgttatc ccatctttat ctggtacaac aaaagaaaca cttgagttag
    19741 tcgaatactg taatgaaatg aacgtcaaaa cattagcttt ggtaggaagt cctaaaacgc
    19801 ccctagaaaa agctgcaaca tattctatct ataacgacgt tttagatgat acatcttcag
    19861 aaaattatta ccttcaagga ctgctgattg ctctcttctt aatgaaacaa agaaatgaaa
    19921 tcgatatcaa tcaatacaat acctatattg aggatttaaa aaaaatacct gaagccttag
    19981 tgcacgctaa agagcaagcg gaaccaatgg caaaatcttt tgcaaaaaaa cacaaagata
    20041 cttcatatca tattttaacc ggtgctggtg atagctttgc ggaagcatat tactatggca
    20101 tgtgtatttt agaagagatg caatggatta agactcgtcc tgttcatgca gcagacttct
    20161 tccatggaac tcttgaatta attgaagcgg gtgtaagtgt catcattaca gaaggagaag
    20221 atgattctcg acctctcaca gaacgcgtga agaatttcgt tcctaagttt acagatgttc
    20281 acgtaacggt tgatacaaag gactatgact tacctggcat ttcggaagac acgcgaaaaa
    20341 ttatttcccc agtcgtctta gctactgtct ttgaacgaat tagtgctttt ctagaagaag
    20401 aacgtaacca ccctttaact actcgccgtt attataagca aatggaatac taatcataaa
    20461 aatatgaaaa aggtgaccgc gactccattg cccatttgat tgcttcattt tctctaatgg
    20521 gagaatagaa tcgcagtcac cttttttagg aggatctaaa atgaaaatcg cagttatctg
    20581 tagtggtggc gactctcccg gaatgaattc agctttatta gcaataaaaa aaacaattga
    20641 tagcgaacca gattcggaag tagtcttttt taagaatggt tatgccggaa tatttaagga
    20701 atgtattgcc tcagtttctc taaaagaagt tgaggtattc aaagctggaa cacttattgg
    20761 aacagccaga tttgagaaaa tgagagatct cgaaagtcgg aaaattatta ttgaaaacct
    20821 aaataagatg gagatcgaca acttaattgt tattggaggt aacggttcct tgaaaggagc
    20881 tgaactattg aaaaatgaat cagaattaca agtatctgtc atcccaggga ctattgataa
    20941 cgatttcccc aataccgatt atacacttgg cttcgatagc gccttaaaca ctataatccg
    21001 atcaatcgat gatttaactt ctactgctaa tgctcatgat cgtattatta taatggaagt
    21061 catgggccga ggctgctttg ctcttccaga gtacgcttcc attgcaaata atacttattg
    21121 ggttgagaga gacgacaagg aatacttaca cttaatatgt aaaaaatttg agtcggagtt
    21181 ctactcgcct atattgcttt taccagaagg tatatctaac aaacaaaaaa tcattgaatt
    21241 tattaccaaa aatttaccaa aagaaaccaa atacttaaat cttgcgtatc ttcagcgtgg
    21301 atcatctcca actttttatg accgatatat tggctatttg tggggtcaat tagcggcgaa
    21361 ggaattaatt gaaggaaaat ctgggatatt aagtcaaaaa aatggtaata taatgttaat
    21421 accttggtaa aaggatggat tactatgatt tttgacaaag taaaagaaca aattcgaaaa
    21481 gtgtatatca ccactgatcg gaaatctgaa ttattgcctt cggaaagaga tttggcccag
    21541 gaattttcgg tgagtcgtcc aacaattcgg aaagctttag cttctttaga agacgaagga
    21601 attatttcta aaaatgaagg tcgcgggtat ctagtgaaac cagcttcaga aaaatatgtt
    21661 gaccacgaac tcgactcttt tatcggattc ttccaagatg cggttcagca aggaaagaaa
    21721 atcagttcta agattatcca acaaaccatc atacctgctg attcaacaat tagtgaatgt
    21781 ttggagattg agcagaatac tttagtgctt gtcttggaaa gaattcgata catcgatgac
    21841 tatccaactt gtattgcaaa aagttgcatt cccctatctg ttttacctga aattgttgaa
    21901 catgattttg ccaaagaatc cctgttctca gtattagaaa gtaggggaat tagattagct
    21961 tatgccaaac gctcaattga agttacaccg ccaacagatt tagaaaacct ttatcttgat
    22021 ttagaaaaaa atgagccagt ctttgtcttt acaagtattg gatttgctaa aaatcagaaa
    22081 ccgtttgaat atgaaatatc taagtaccct gcttataaga caaagtttga aagtactgtt
    22141 tccaaataat ttttaaatat gcttaaatgt ataaagagcc tcgcggataa cagataaagt
    22201 gtctttccgc aaggctcttt ttctacttcc tctttatctt aatccgctaa gtagcctaca
    22261 gtccaaacat tatcatctaa ttaaggtgtg tggaggtact cctctaccct aagtcaagga
    22321 tagtcgacat agttgttaat aagtcttcaa agcaagcctg aacctttttc attaggtgtg
    22381 cttacttcac atatgacgct atcaaaaatg atagcacccg ttgaatcaac caattttctc
    22441 taattcatat ccccattcaa acaactatat tttaaaaact tttttaatct ttgattatgt
    22501 gtagcaaatt tgtctaattt ttatttcttt gcaaaatttt tccaaattta ccagataaaa
    22561 gtaattcata ttcatgattg agtcctttac caataaaacc tatttcgcaa aaattatttg
    22621 taagcattaa ggattttctg ctgactttca ctggaaggat aacccaagaa ttctgaccaa
    22681 agtgaaagat taagaaccat ctttgccagt ctatctatgt gctattgtaa ctgtcaacat
    22741 catgcgggca gttattatta tttatttgaa taatattttt attataaatc agttttttag
    22801 attgtgcata atcactaatt gctgtgatta attacagaat atggctctct gggaaaatgt
    22861 gcgcgtcatg tattcaattt taaattctaa tttgtacaaa atgaatcgtc acgactcgat
    22921 ctgacgcaac tccaaatggg ggcgccgacc tgtaccaagt acagtggcat gttctttaaa
    22981 catttgaagc aaaatctgtc catcaaaaac ctgtattttc aaagtgaaca aggtgcgcta
    23041 aatcaggaaa tacttacact gatcatttct taatttacct acttaaccaa aatttaaatc
    23101 aatatgaaag caacactttt caaactaaag aggtcttttt accgcttgat gtttgcttcg
    23161 ataaaaaagt tgatgtcgga ttagttttca ttttaataaa acgtactatt cctgaaaaaa
    23221 atttatccac tactaagtat tcggattttt ctaattttta acaaaattta tttcgaaatt
    23281 ttctgatatt atattcgcaa cattagtgaa aattcttaga taaattcatt ctttcaatct
    23341 ccgaatttta gatatatgtg aaatatctgt tttttgaatg tttgcataag ttaaaagatg
    23401 agcgtgcttt agtattcttt agaatagctt cttatacagg ggaacgaaaa ggagaaatac
    23461 tcggactgac atgtagagac atagatttcg ataatcaaac tatctgcttc aacaaaacgt
    23521 tagtagaact atccgagggc tctctatgta ttaatcctac aaaaacagac aaatcaaagg
    23581 gagtaatttc aattgataat ggaaccttac gtattctaaa aaatggaaac gttctgtaac
    23641 ataaaagaac ctttctagag ggattcggat agataatata gatgatatac ctgtaatcag
    23701 cactatcaaa gggaataaaa aaagggactt cctttacaaa ggctacccta accatgtcat
    23761 ggataaagta gcacggaagt tcccagatat gaagataatc aaagtccatg gcttcagaaa
    23821 gactcatgct acccttctct ttgaagcagg tgctcaagta aaagatgtac aagataggct
    23881 aggacattct tcttctaaaa caatcatgga catttatatt aaagtatcaa aaaatcggca
    23941 aaatgaaaca gtcgaattat ttgattctta catgaacgga tgataagcga ggtcaaaaac
    24001 ggagtcagag gatattatgg ggataccttt ttacctctta atcctttaat atcaatcctt
    24061 taagcttcga cttttgcaat cttcccttgt tcgatataga cccaaatcga tctatcaata
    24121 attatcaaac tgagtcatat agagacaaaa atttcaaaaa cgacctatta attggtcatc
    24181 gaatatcatt taaaatcaat acgacggagt caaacacgga gtcaaaacaa gcccgctgag
    24241 agtaaaaaat atacagtccc gtaggtaaag tgccacaggc ctgtatattt tttatttgag
    24301 attacctgaa atgattccat ttaattttta tctcattttt tcctactagt tagtatcaaa
    24361 tactctccta ttttctcaat aaactatttt gggttacatg gtatctgaaa cagctaaaac
    24421 cttgtcatta tagcaaaaaa tcactttatc tccgtttcat ttcagataca tttcacttaa
    24481 gcggattcca aaaaaacaaa agaaaaagga acccctagtt agcctatgct ttttattggt
    24541 tcctctcaaa acacggtcat ataacaacgg ggtggggtaa atgaatgaaa aaggtggagg
    24601 aaacattatt caatagctgt cttaaatact tcgttataaa atgtgaacaa ataaaagaca
    24661 tctatcccta ttttctacac ttccacttga ctaccatctt tgaatcgtac aattttctta
    24721 actcttctcg tttacctcaa ttcattctca agtggaatca actcatcatc taacatttta
    24781 cgccatgctc gcttcatttc ttgtgggtat ttaaatggct tcgcttctgg atgtttggct
    24841 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
    24901 cctggtagat attgcagatt agaagcatgt aattctttct cagaaacaaa ccactcattg
    24961 gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat agcgacgttt aatgacaaag
    25021 atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg tcggtaaact
    25081 tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc ttctcgtgca
    25141 tctgaactat tcgcagcata agttcctagt aattgatcaa tataagttga atcaatatca
    25201 tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc tccttctttt
    25261 tctggttctt cctctattaa tgaacccttc acggtctcgt acacgccaat ttgttcttcc
    25321 aaaagttgaa tttgattgtt caaagttgga gatttcacca tgtcatcatt gtaatcgtca
    25381 taagtcacta acgattcata agcacgattc aattcttgaa atgctttgac gtactcgata
    25441 cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc ttttaactta
    25501 ccatatgctt gtttgaatct ttttttggat tcctgataac ttggataaat caaacctgat
    25561 tcttcttttg cttttgaata cagttttgtc gcatcctcga cattcttctc cattgtcgct
    25621 ggcttacgaa acgtcacaat caatcctttt gtcttatcag gatacgtacg attcgtacgt
    25681 gaaaaagctt ggatcaatcc tgcatactct agattacgat ccacaaatag ggtttgaatc
    25741 gtaggggcgt caaaacctgt taatagacga tccactacaa tcgctaaatc gatttgtttc
    25801 ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc tccgttataa
    25861 cgctcaatgt ctgctaatga ccaggaagta tcataatact gattatattc ctcaataatt
    25921 ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa tgaataggta
    25981 atagcgattc gtggaaaatc aggatcctcc atcgttcgtc cttcacgaat gagctgattg
    26041 ggaaactctt ttgttaacca atcaggctct ttcgtcatct ctttgatggc ccgataatac
    26101 tttttggcca tgtcaatgga actcgtggtt aagattgcag atttcgttgg cctgccattt
    26161 cgaaaatcaa attttgtata ggcattatct ggacggaaca ttttatgaat gactttttgg
    26221 atatgttcat ctgcttcaaa aacagctggg tccaaataac tttctttttt gacaggttcc
    26281 atttgatcaa tcatacgatt gatctgttct gatgaatact ctgcatatgt ttctacttct
    26341 tttaatttcc gatagatttt attctctagt gaagtcggct ccacggtgtt ttcatgctct
    26401 acttgaaatc ctaagacaga gccatcttct agcgcattct taatggtata ggtatgcaag
    26461 acctctccat actgatcatg ggttgtacgt gccaattgcc ctttggcttg tttccgattc
    26521 tcaggaaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa catttttttg
    26581 atttcttcca tattttcagc acttaacgct cgatgacact catctactac aaacacgata
    26641 tgttggccca ttaatttttg gaagcgattg gttccttttt tctcttcttg tttctttgca
    26701 tatttcaacg ccgcatctag tttttgacgg gttgtaatga ttacaacatt tgcattggca
    26761 tctgccaaca gggtttcact taattctttg gcacttcccg ttccaacgat caagctattc
    26821 gctttggcgt tcccggaaga aatcccagta ttaaattccg aagcaaattt tgtaaactct
    26881 gtggtggttt gattatctaa atctttgcgg tccactaaca tgatcgttcg atcgatcccc
    26941 gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc tgaaccagtc
    27001 gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca
    27061 tgtacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc actgacgatt
    27121 gtataatccg caatcaaacg atgagcatcc gggatattta acacttgttt acaaaactca
    27181 tataagtttt ctactttttc attgtctttc gttcgccaac taaataaaaa cttagggtgc
    27241 aagtctttcg gtaaggcatt tgcaaaatac cgagtggttt gttcattgga aaccacaaac
    27301 aactgtaagg tcgaaaagat attgttccga aacatacctt cttctgcata ttttttgatt
    27361 tggttaaatg cctgataaac accatctttt gcagttactt gtttcagctc gatttggaca
    27421 atcggtaatc cattgattaa taaggtgacg tcaaaacgtc gatcccgtgc ttctatgtta
    27481 cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc accaatgtct
    27541 tgattggaat aaaggataag tgagacagaa cccaattgcg aatcttcccg ttcaatcgtg
    27601 atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg ggttttcgtt
    27661 cgcagcagta attccgtttt gatcgtttca aattctttat cggtcaaagg aaaatcattc
    27721 agttctgctt gattatttga gataattttt tggcgtaaat tgacccaaag gtcttcttct
    27781 gattttagat ccggacgata cgtccactga ttatgccctt ctccaagtac acgaatcaac
    27841 tgttcttcga cttctgcttc ctcacggtga ctaattttca ttcttcttcc ctcctagaca
    27901 aacatctttt gtaagaagct ttttttcgtt tcttttagta aatctaactt acgttgatga
    27961 agagcgatcg tatcatctag ctgtttgaag aaagtaccga ttttttgttg ttcatccttt
    28021 acaggaacct taacactgat attctcaata tcagaactgt tataagaagg caatgcccct
    28081 acttgggagt atcttggcaa atttatagtt tcaaacaata ttttaccaaa atagatatcc
    28141 caagtattat taaataaata agccatggta ttgttatcta ttaagaaagg gcttgtgacc
    28201 aaacgttttc tattaagcat tattgctgcg ccaactttag caaatataat cgctggcaca
    28261 tcttttattg gcttccattt tttacgctca atttgctcat tagatacata atgatttgca
    28321 tttctcattt catactcatt accaatgtta ttcatgtcgg aaactttaaa aaatggagta
    28381 ccttcagaac caccttgctc ggcatcagga aatccaattc ccgattgagt tttacctaat
    28441 tctcccaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat ttctggcact
    28501 tttgctccgt tttcagggaa cattttttgt aagaatcctt ttttcgtttc cttcagcaaa
    28561 tctaacttac gctgatgaag agcgatcgtg tcgtcaagtt gtttgaagaa ggtgccgatt
    28621 ttttgttgtt cttcctcact aggaaacatt gctactatat ttttaaactt ttttagagac
    28681 agaacatgtt gacctgttcc ctctgcttga atagataatt gtcgttggat tccattgtta
    28741 atacgtctta agaagaagtc ggaatcagca gattttaaag taaatacagg ataaaaatag
    28801 cttataatcc ctctatcaat aatgtcatta cggttaaaca caaacacatc attatcactt
    28861 cgactacgga acgtgaaata tcctctaggt agtacaaagt aaccaatatt attttctgta
    28921 gttacttgcc gatttgcaaa atagtcttct tgcaaaacta tccctttttg ttgagatgaa
    28981 gttagcatag gaaattgatt ttgtacactt gtcttttcaa cataatctac aacgaagtcc
    29041 cccaacttcc tctgttccca atcatctgta aatcctggga aacggatttc tggttgtgta
    29101 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
    29161 tgtttgcagg gaagagatag cttctaaaag ttttttttct agcacttgct gttcttgtcg
    29221 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
    29281 atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
    29341 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
    29401 gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac tagcatcaat
    29461 gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg ttggaatcga
    29521 tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc catcttctag
    29581 taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt gtggtaagac
    29641 gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa acgcaaaatc
    29701 tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat ctaagaaagt
    29761 cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat caaatgtgta
    29821 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
    29881 ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
    29941 ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta cgtttaacat
    30001 caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt ccttttgatc
    30061 gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa attctccggc
    30121 tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat ccccgatcac
    30181 gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt cggtaatcgt
    30241 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
    30301 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
    30361 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtgc
    30421 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
    30481 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
    30541 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
    30601 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
    30661 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
    30721 taaagttgct atataccctc ttattttagc ataccccctt ttataaaaag caaaaaaaaa
    30781 aagaaagaaa cttattcttg ctaataaact acgcgaaagg agtctaacgt tgtttgatat
    30841 aacttagact gatcgttaac ttatttttta tatttctaca tagttatctt tagaaccatt
    30901 tcagatacat atgaaacaat tacatatgat tttttcataa aaaaacagta ctgtctagta
    30961 gtataaaata tacaaatttc ttaaaaattt tttcactaaa atcactacct gaaacagtct
    31021 aaaacactta tatatcaacg tttaatgaaa ctatctccgt ttcatttcag atacaaataa
    31081 atatagtcgg gctccaaatc cgaaaaaaaa tacccccagt agcttatcct aagctactgg
    31141 agatgtataa gtacggtcat ataacaacgg ggtagggtaa ataaatgaaa aagatggaag
    31201 aatcattatt caataacttt tttaaatact tcgttgtaat cagttacctg tccgttctta
    31261 aataccacag aataatcttt tttcccattt tcaagtaacc gaagtggaat gtctttacca
    31321 acttgctctg tcactgcatt actgatacct gttagattag ctaataaatc tgataaagat
    31381 tgagcatctg tagaaatctc tgtatcagta tttatcgtga ttcctttatc ttcatttaca
    31441 agcttccaag taggtggtat agctgtgtat gtttcgatga atgaatcaat tctttcatct
    31501 atcgtcttcc cagtatctac ttctgtctca ttactcttag atgaatcgat tctttcatcc
    31561 atcgtctgct ccgtaactgc ttctgtctca ttattctcac cggaaactct cttcacaccc
    31621 aaacttatat attgtccatc tttagtgtgc atagttaaaa ttcccacgct tttactaata
    31681 aattcactat aaccagttct ttcaatagag acttcaatgt atttcacact atcatactca
    31741 aaagaaaaat agacatgtaa aagcattttg ttgtttacat ctacataata atcctttgac
    31801 tctacttgag accttcctat acggaataca gctttactgt ctgatatctc tataccagca
    31861 tccttattat taatctgcca atctccaagt agctcttctg gcaagctagt agtttcttgt
    31921 gaacacttta ccacttcact ggtagaggtc gaaacattca cctccgcact gttagaagaa
    31981 ttttccaagg aatcaggagc cttagcacaa ccaacaagtc tttatatatt gtgcagccga
    32041 tattttcttc attcagtaca cccttgaaat atgcagtgtt agataaagca gttcgtcctg
    32101 atttgctgag gcacccatct cttcttgaac gtactccttg attttcaagg ccgcccgata
    32161 ctctttcgga aaacgctgct tcactgaagc cagcaaaaaa tcatccttct ccggcaactc
    32221 ttccttctta atcattcggg caataaaaaa ctgtaaatgc gtcacaaatc ttaagtaaag
    32281 catcgaatct ttatcaacct ttttttcggg aaacagatat cctagcatag ccaagatatc
    32341 ctttagtgct cttaccgaga gcatggtttc ttccatattt gcttgttcac tttgggcatt
    32401 tacaatgtga aacgcaatgt ttccagcctc ctcttctggc aactgaacat tcattttttt
    32461 gttgatcaat tggatagcat agcagccgat ctcaaattct tttgggtaat acttttgaat
    32521 ttcaatcagc aggcgatttt gaaaaacaat gcctttttga ctgcgttcaa tggcatgctt
    32581 caagtgatca accaaggtaa aaaatagact tttattcagc ttaaagtcaa acttggcgtt
    32641 ggcataatcg ataattcgct tgacgcaaat aattagatca tccggagcag cctcaaacag
    32701 tttaatgtaa tcctttttta gagcatcaga aagtgaatct aatttaaata ctttttggat
    32761 tttgtcagcc ggaaccaaat cgcctttctt tagttgaaat ccaatccctt tccccatcaa
    32821 aattacttct ttgccttgct tatctaagca aagaattaaa ctgttgttta acgttttaat
    32881 tactctcatt agattcaccg ttctctctaa gctaagccat ttcttgcgat tactttttga
    32941 taccaaaaga agctttcttt ttttattcgt tttaattctt taagattctc atcggttcgg
    33001 tcaacatata taaaaccata acgcttattg aagccttgat gggtactaac aacatcaatc
    33061 gctgaccaag gtaaataacc aaaaacatct acaccatctg ttaaagctgc ttgaatagcc
    33121 agaatatgtt tggctagata atcaattcga tcctggtcat taattcgtct atcttcctca
    33181 actatgtcgg gtgccccata gccattttca gtaattaaaa taggtaaatc atagcgatcg
    33241 tacaatttcc tcaatgttaa tcgtagtcca gtagggtcaa ctacccagcc atatggcgtt
    33301 ttatctacat aaggattttc agctgcccgg tagacacctt cttctcccaa catgatctgt
    33361 tgatcccccg ctctagcgga aacatctgat ccgtcatttt tgctggcagc gatggtggca
    33421 gttgaataat aattaatcgc aataaaatcg ggtttaccgg aggataaaat tgcctgatcc
    33481 tctgcctcaa ttttaggata taatccacga tcattcaaat agttttcaaa caaataattg
    33541 tattttcccc gaactgctac atctaagaaa gaccagcctc tcaatgtttc ccaattatgg
    33601 gctgcaattg catcttctgg actggaggta gcttgataca tagccgttaa attgattgct
    33661 ggaccgattt ttcctttaag attcaactga tgatacaagt gaattactct agcttgagct
    33721 aataaaacat ggtgattaat ttgaaagagt tctttttttg aaggcagctc tccacttggt
    33781 ggcaaaccaa tcgctccagg atgaaggatc atcgtgtttt gctcattaat tgtcagccaa
    33841 tagttcacct tgtcaccata ctcagtaagc aatacctgta catagtccac aaaataatcg
    33901 atagttgctc gatttaacca accgccctgc tcctctagaa aataaggtaa atcgaaatgg
    33961 tacatggtga caatcggttc gattccctga ttcaggcatt catcaattac ttgatgataa
    34021 aaagaaattc ccttttgatt ggttttatta ttttcaatat ccataattcg cgtccaagcg
    34081 atggaaaagc gaaaagcttt taagcctaat tctttaaaca gctgtatatc ttctttatag
    34141 tgatggtaaa aatccgaggc aacttcaaaa ttagctaccc cttctgtatg ctgatacttg
    34201 tcaataatag acggttgttt cccatcctcc tgataggcac cctctgcctg gtaggcacta
    34261 atggaggcac cccataaaaa cttctctgga aatgctgaaa gctgtttgta ttgcacaaaa
    34321 atcctctcct taactaattt gcatcaatgg ttcgtgttcc tttaagcttc cgcttgcttc
    34381 actaattcct gcgtaatttt cactgttggt cactaccaca atcactgtat catcgtagcc
    34441 agcgtttcta atggcagcga aatcggcgtt gattaataat tgtcctgctg acacctgatc
    34501 tcctgctttt acaaatactt caaagggttc cccttttaaa ttcacggtgt cgatccccac
    34561 atggatcaat aattcaatac cttctttaga acgtagacca atcgcatgtt tagattctaa
    34621 aatagtaatg acttctccct caacaggtga ataaatgctg ttttcttctg gatgaatggc
    34681 tacgcccttt cccaatagtc ccttagaaaa gacagcatcg ttcacttcag aaataggaca
    34741 cacggttcct tttaccggat tttcaacaat gactgcttcg gctattgccg tttgctctga
    34801 ttcatcagaa gtatcgtcaa atccaattag ataagcaatg ataaaggttg caacaaaggc
    34861 gattacacac gtaataattg cgtgaacaat attcattgga ttttctccaa taaaggctgg
    34921 taaggctgct aagccagggg aaacaaaggc atatctgact aaaccgctca agccagcata
    34981 caaaccagca attgcaccac caatcatgga agcaactaat ggttttttca gcttcaaggt
    35041 tactccatag agtgaaggct cagtaattcc cattaaagca gtgatcccag tggacacagc
    35101 tagctgcttc aactctttgt ttttagtttt cgcggctaca gcaaaggtag cacctgattg
    35161 agcaatatta gaagccagca ttcctggccc attgatcatt tcagaacccg tactgctcaa
    35221 ttgcatagta gcgattggcg tcattgccca agctgtaccc gtaaccacca aaaatggctg
    35281 caaaccaccc atcaaaaatg gaattaacca agctgctttg ccgttaatcc attcagcccc
    35341 cattgctata agatcgttca gcaacgttcc aaacggtcca atgacaacca acgctaaagg
    35401 ccccataatt aaaaatacca gcatcggctt agtaaagaat ttaataaacg aaggcgaaat
    35461 tcgatccgca aagggctcta cataggacat gatccaaaca gtaaagataa tcggtaaaac
    35521 tgatcccgca taatctgcag ctaataccgg aattcctaga aaagaaacgg catcgccagt
    35581 agaaaaaagc tgtgaaatat ttggatgaag caaaatgcca gcaacagtaa tcgccaaaat
    35641 tgggttgaca tcaaacttta tggctgcccc ataggctaat agtactggca taaaataaaa
    35701 ggctgcatca gcaatagtat tcaaaatgat gtaagtttga ctactgtctg atagtacacc
    35761 agtcaaagtc aagacggcca aaatggcttt taccatcccg gcacctgtta tagctggaat
    35821 catcggtgta aaggtggtag aaatgacact aatcaatctt gaaataatac tttcttgatt
    35881 gtctgaagag gggccctttt taattgaagt tcccacttcc ttttgaattt ccttgaaggc
    35941 tgtttggact tcattgccaa tcaccacttg gtattgtcca ccgctatcca ccacactaat
    36001 tacaccaagt gttttttcta aggctttcgt atcggctttc cttttatctt tgagttcaaa
    36061 acgcagtctg gtggcacaat gggttaagct gaccacattt tcttttcccc caacctgttg
    36121 caatacattt cttcctactt ctctgtaatc cattattctc tctccttaaa gcagtaaaaa
    36181 agaccaagtc aaaccacgcc ccaatcaaca ttgaagtgtt agtttgtctt ggtcttgcct
    36241 gcataatcag taacatgcca ataacgttat tttgctgtaa tccaataata gcacattatg
    36301 atagcgcaat caacagggaa tttaattttt tatcttttcg tttgaccgta tagggtaata
    36361 taacagttag atcacatcaa ctagggtata tctgcgtata cttactgcta aaataagtat
    36421 atctaatatt aactatacca gtaaaaccat tcctacataa ccagcgggaa attcccaaaa
    36481 tcgtgaaagc atcaaaaatg catataacgg atcacacaac ttcaacaatt ctgctcttgg
    36541 gaaaaattga tgaatttggt gagtaaagga tggaataaac agcattccaa caatataggc
    36601 aacaatagtg atacttgttt ttttggtaaa aatcagtaag caatagccaa tttcaatcat
    36661 tccgagatat aaaggtatcg atagaatgat ttgggctagt attcttaaaa attaactagc
    36721 aaatgtttaa actgatttat ttctaatcta catgtagtaa gtatttcaat tccaccaatt
    36781 atcaataaaa ataccaaaca tatccataat aattttttct ttttcatttc ttttatattg
    36841 ttataacagt gaataacata aaaattcccg acaatcattg taatattttt ttgtcacctg
    36901 tattagtatt taatttatag gaaactataa aaactcccta gtgaatttac acctttggga
    36961 gttcctgttt ggacggtatg cataatacat ttaaatcaat ttaacattaa cacctcagca
    37021 tatggtatct ccacatatat acggtctttc tttggatagt actcaatatt tatgttgata
    37081 gcacttccat tgtctaatat agctttagcc cggtatgtgt tatctccatt ttcatgagta
    37141 atcgtcacat catcacatga tcgatttaag ttgtactgtt cttgtaaaat ctgtgttacc
    37201 aatggttttg atgacttctc taatccagat gtacctgtca tttttgaaaa aataatacct
    37261 ataatagcca agacaacaat acctataatg acaaacttct tgttcatatt tactttattc
    37321 aactttttat ctcctatctc caaaataatt tgctcttatc cctcttatca gtacattgac
    37381 atccctaaaa gtacaccaaa gattgctcca gctacccctg ctatcatcaa aatcgtaccc
    37441 attttctcat ctttttctcg atataagtag cccataataa cagcaacagc accaaataca
    37501 acaggaataa atgctaacga tactaccata gaaatccaac caataatcaa atacagcttc
    37561 cctgtctctt gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt
    37621 ttctttactg tgtctgttcc ggttgtctca tctgtttctt gtagtgctcc acaaaactgg
    37681 caatacttac tttcatctgt tatttctttg ccacattttc tacaatacat gtcttctcct
    37741 cctaaaatca ttcaattgct tattagttaa ctgtttactt tattaaatac atcagaaaac
    37801 agctcattca ttggtacact taatatctca gaaagcatct tcatttgata gggtttagga
    37861 atcgttctcc ctgtttgcca cgaacttaca cattgctgtg taacattcat tttttttgct
    37921 aactccgttt gagaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc
    37981 atgattctcc cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat
    38041 ataaaaaact tcaaatacca ttacaaatac aacaacttgt tgtactatac tgagatgggg
    38101 gtgtattaat gaaacagaaa caatatgcaa aggtcattag agaaaagcga aaagctctcg
    38161 ggtggacaca aaaagaatta gctgaaaaaa tcttctctac acaacaggca gtagcaagat
    38221 gggaaaattc tgtaacagag cctaacctcg atagcttaac agctctttct agagccctag
    38281 gaacccctgt aagccatttt ctagacaatg ttgtcgtaga attatgagga agagtttttg
    38341 gctctctatc gttctcttag cactgaagat gcagttcgaa caattgacta tatgaagcta
    38401 ttaaaaaggc aagaaaatga acgcaatcag ctattgaaag attgaaaaat aaataaccca
    38461 ccgaacttta acggtgggtg tattgagatg aaataagatt aagagctatg gataacctgt
    38521 ctagtttaat acaattgggt cttttctctt ccttactctc cgtccatcat ctcttgttac
    38581 acttgtaaat tgatacagag gaactatccc agctcctgat aatatcaatt cttttattgt
    38641 ttcatctatc cctgcccctt tcgctaaata tattaaaaaa ctactacttg ctttcacatt
    38701 gtatagtttc ttaatatcct tagaagctcg ttcaagagtg aatccttgtt taacaaagta
    38761 gcctctgttt tttattaaag catttttcac gctgttatcc ccaacattac gtaggcgttc
    38821 acaaacagtg ttaataagtt ctttctctgt cattagatat gtcccttgcc acgataagcc
    38881 ctgtaaaact tcatgtgctt catctaaaat caattgtttt tccttgctga gttctgatcg
    38941 ggaattttga ggaaatactc ttttagcctc tttttcgtta aaacatatct taacccattc
    39001 ataagaaaaa tattgatatg taaaatgaat tttttccaga agtggaagga tatcttcatt
    39061 caatttttga atcacagaag ttgcattaaa aacgtctaat cgatagacat tgcttctctt
    39121 atgattaaag tttcctaacg ctttctcttc ctttaaccta tttaggtatt cgagcatctt
    39181 caaaggaaca tcttcatcat ctaatttttt tatcaatcct ataaaagtca taagtgatag
    39241 taacttatta catttatcta tgtcataagt tttaccatag taagaactca tttttttagc
    39301 taattgttcc cccgacaaaa tacaagtcac actttcctta ctatcatcaa aaaaattaac
    39361 atcagaaagc aagacatcat agacacgtcg catttcctct agataattcc ttattgtcct
    39421 gaaaagtttg tctttcattg attcaagagt gtcaaaaagt acttttgatt cctctttcaa
    39481 ttttcgaaaa tgtgtaggat acaagtctag gttctctaat agccaattaa atgtttctac
    39541 ccgactgtct ttctttaaaa ttagcatcac aactccaata aaattttcac tgatatcaca
    39601 attactggaa tgacaataaa aaatggaatg cccagtttta ctggtaaaaa tactagcaga
    39661 gggattatga tcgttgtgaa acagacatct taagttttta cttgacttaa gtaatttatt
    39721 catatcaatg gttaacaatc tttcataaat ctcattaaaa gacatgtcgg aggaatcaaa
    39781 caggcagtct ttaaaccact gtttcatttc ttctttgtcg tcatttttta tcaacttaac
    39841 aaaatcggtt tcattgacta gttcatactc tctccctgaa tcactttgac aatttttaga
    39901 acatgtcatg gtaaaatcaa tcggaagctc agtaatatct aacatgttgt cttcattaat
    39961 tattatcact gcatctgact tatatcctcc aaaaaacatt ctattcgaag catttacatt
    40021 gtcatcacac ccggggatgt tctttttcaa gtattcgtaa acagcaacca ttttaccata
    40081 gtcagtaatt gcttctttta atagaaagac aatcttgtat ttttctagat tttcagagta
    40141 ccgaatactt ttttgtatcg caaacgcaga attatgaact aaattgatag ataaaacctt
    40201 ttctacgctt aagtatctac tatcatcttt actcaaatct actaccttcc ccttctcatc
    40261 tttaacagtg ttatcgacat ccagaataat tactctggtt tccacaaaac tctccttgtc
    40321 attgccttta ttctttgtct tgcttggaat aaatgattgt gatgaattta gtatctctac
    40381 aaactcatct cttgtaactt ctctatagtt atctaaacac ctatctctta catcagtttt
    40441 ttcttttttt gtaactggct tctctaataa tttttcttga tctacataaa ttggaatatt
    40501 aatcattgat gtttccattt gtgtttcctc cttataaaat tctgttaaag actaaaacat
    40561 acagataata aaaacaaact ctttattact atatgtttta gtcttatttg tttactcaac
    40621 ctttataccg ttccatgaaa ctatcaatat cagatttttt aaatctaatg accgtatcaa
    40681 tttttataac aggtaacccg tattgagaaa catattttct aatcgttcct actgatacac
    40741 ctagatattc acttgcctgc tgatagtcca tatactctag agtgactcca gaaataatgt
    40801 cttctagcat aggctttagc actgtaatta tctctttcac ttgttgttca acttgtatag
    40861 aaacaatgct atcaatgatt tgtagagaca tatttccccc tcctttcgtg attgcttgaa
    40921 caaattttct aagaattatc ttcgtatttt ttgctcaaaa taaaagtatt ttgttattaa
    40981 ccgattaatc aaacaaaaaa tgattaccag ttcatcgaca tacacataat acctcgataa
    41041 aaaaggggtg tcaacatctt tgtaaaattt atttacaaca cccctttttt gaggtatact
    41101 aattttagga ggtgtggtca atatgataaa acgtccacca ataaattatt tagaacgaaa
    41161 aaagatactc ggaacaaaaa taaaagctat aagaaagagt aaaaaattaa cacaaccagc
    41221 atttggtctt atgattaaca acggtcaatt aattgacaaa aaaactatct acgaatggga
    41281 gaagggaacc tatttaccaa taccagagcg attatcccga attgctgatt tgggaaatat
    41341 gagtatagag gagttagttt gtggcaatgt agaagaatat attttgggaa ttattcttta
    41401 tagagatagc attgtactag atggtataac atttccagat aaaaacctgt tccagcatct
    41461 tcgtcaacag ttcccaccag tacactctaa tttagataca tggctggata gatattccaa
    41521 attagaacca gagatgcaag aatttattgc taacaagact tgtaataaag ttaaaaatga
    41581 aaaaattagt cttttcaata tcttaaaaat agaagaatta ttcatcaatg ctattgtaga
    41641 aaaatttgac aataacattc ttttcctcac tagcagtata gaagaactac tagaaagaat
    41701 ggtagatgaa tggctaccta ttcaattgaa agacatgtct tatcccgaag aagctgtgag
    41761 agaaattact gataatataa acaagctaga acaaacgatt tcatcaattg ggaaaaaata
    41821 cactaagaaa aaaatgaagg gaggtgatac catctagtgg taagtactcc ttgtaaacta
    41881 agaggagatg atttaatggc aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg
    41941 tggatgtata aaggacacat tgccaccgat ccacgaactg gtgaaaaaat aaatactacg
    42001 agaagaggtt ttagtagcaa acaagaagct caaaaagatt atgacgagta cagacatcaa
    42061 atattatacg gtgttaaaaa gaaagcacct gatatgacat ttgaagacct gtataatgaa
    42121 tggataactc atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac
    42181 gcaaataatc aaatacttcc agcatttggt aagcttaaac tatcgaatat ctctgtccca
    42241 tattgtcaaa aagtggtaga tgaatggcac agtaaatatg aaagttacga ttatatgaga
    42301 aaacagactg cacaaatttt acgctacggg gttgcaatgc aatatattga caataatcct
    42361 atggaaaaaa cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa attttactca
    42421 aaagaggagc ttaataattt actggatgct tttaaagatt ttggtaacat gaaacaatac
    42481 gccttcttca gattattggc atacacaggt atgagaaaga gtgaagtact cgctttacag
    42541 tggaaagaca tagacacgtt taataaagaa ctacatgtca ataaaactct tgctgtagat
    42601 gaatttggta aagttataat tcaatctccg aaaacaagag cttctagacg agttatctct
    42661 ttagatactg agactctttc tatcctcaac aactggaaat tacagcaaaa agaagagtat
    42721 ttaaaacttg gatacaatac tagctctaaa gagcaacatg tttttacaac agttaaaaac
    42781 actctataca ttccaaacac tgttaatgat tggttaagat atattttgaa aaaatataac
    42841 ttgcctcgta ttacacctca tgggtttaga catacacatg ctagtttatt attagaagca
    42901 ggagaatctg tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa agtgacatta
    42961 gacatctatg cacatattac aaataatgca cctaagaaaa caggtcaaga ctttgctgat
    43021 atgatggctc atcagtaaaa gagctggtca aaatctggtc atttctagta tacaggcaaa
    43081 acaaaaaagc tagaaccctt atatatcaag ggttctagct cctataacag gtttacccaa
    43141 cactgccttc catctcataa ctgatcaatc gattgagttc gactgcatat tccattggaa
    43201 gttctttggt gaatggttca acaaagccca tgacgatcat ttcagttgct tcagattcgg
    43261 tcaggcctcg gctcatcaga tagtagagtt gttcttctga gatttttgag actttggctt
    43321 cgtgttctaa agagacttgg ctgttgtgga tctcattgaa tgggatcgta tcggatttcg
    43381 accattcgtc catgatgatc gtgtcacatt cgatatgaga gatcgaaccc gcactgtttt
    43441 tggcaaaggt tacttgtcct cgatagttta cttctccgcc gtcttttgaa attgatttag
    43501 agacgataga gctagatgta tttggtgcat tgtggatcat ttttgcaccc gtatcttgga
    43561 tttggtttgc tcctgcaaag gcgatggaga gcatggtgcc tcgtgcacct tttccatcta
    43621 agtaaacact tgggtatttc atcgttgttt ttgcacctag gtttccgtcg atccattcaa
    43681 cggttgcgcc ttcgtatgct tttgctcgtt tggttactaa gttgtacaca ttgtctgacc
    43741 agttttggat cgttgtgtaa cggcaatacg catcttttct tgtaaagatt tctacgatgg
    43801 cggcatgcag gctgttactt gaataagtag gtgccgtaca accttcgaca tagtggatgc
    43861 ttgctccttc gtcaacaatg atcagcgtac gttcgaattg ccccgtgttt tccgcattga
    43921 tccggaagta cgtttgtaat ggaacatcga ctttgacggc ttttggcacg taaataaagg
    43981 tcccacctga ccatacggct gaattcagcg ctgctaattt gttgtcggtt ggcggtacaa
    44041 gttttgaaaa gtactctttg aataattcag ggtattcttt caaagcagag tcagtgtcag
    44101 taaagacgat tcctaacttt tcgaattcat ctttcatatt atggtacacg acttctgatt
    44161 catattgtgc ggaagcaccg gctagataag ctcgttcagc ttctggtata ccaatctttt
    44221 caaatgtatc tttgattttt tctggtacgt cttcccagtc acgagcgggt ttgtcacttg
    44281 gtttttggta atatttgatg gcatcgaaat cgatgtctga aaggtcgggg ccccatgttt
    44341 gcattggcat tttgttaaat gtttctagag atttcagacg gaattcaagc atccattctg
    44401 gttcgccttt gattcttgac atctcacgga tcacttcttc tgtcaagcct tctcctgtac
    44461 tgaaaaccgg ttcgacatca tcgtgaaacc cgaatttata ttcttctaat tctggtacgc
    44521 ccatgttctc actcctcttc cttgtggtaa tgatggaaag ctgcggtacc atcgttttcc
    44581 attgcttttc ctagtgcttt ccaggcaagt gtcgcacatt tgatccgagc gggaaattta
    44641 gctacaccac caagcatagc agcatcgcct aattggtcgg gatctttgac ttcttttcct
    44701 tggacaagct gtagaaagtc tacagttaaa tgttctgctt cttctattga ttttccgatg
    44761 acggcgtctg tcatcatcga ggcacttgcc gtactgatgg agcagccact gccgtcaaag
    44821 gcgatatttt tgatgattcc gtcttttaca tcgacttgca attcgatcac atctccacat
    44881 gtggggttgt tcaattcgat cttgcttgtc gagtcagtca agcttccacg atgatgcgga
    44941 tgggaagaat gatccaagat cacttgacgg tataaattgt ctaatttaga tagtcccatg
    45001 ttggaaaaac tcctttgttg ccatgattgc ttcaactaaa cggtcggcgt cttgcgttgt
    45061 gttgtagaaa taaaagctcg cgcgagcagt cgctgatact tcaaggtact tcaatagcgg
    45121 ttgcgcacaa tgatgacctg ctctgacggc taccccttcc atgtctaaag ctgtcgcgac
    45181 atcatgggga tgtaacccat ccaaattgaa tgcaacgact cctgtccgaa aattcgggtc
    45241 ttgtggacca taaacagtca atccatcaat tgcttgtaat tttggcaaca cataagcggc
    45301 tagttcttct tcatatcgat ggatattgtc cataccgagt ttttgcaaat aatcgattgc
    45361 atggcctaat gcgatagcgc cggcgatatt tggtgtccct gcttcgaatt tccaaggtag
    45421 ttctttccat gtactgtcgt ataattcgac aaaatcgatc atttcgccac caaattcaac
    45481 aggttccatt tcttctaacc attttcgttt accatataaa acaccaatac ctgttggtcc
    45541 gcacattttg tgtccgctga aagcaaagaa atcacagtct agtgcttgga catcaatggc
    45601 catgtgcggg gcagattgag caccatctac tacgatgacc ccgccgtttt catggacaag
    45661 ttcagctaat tcttgaacag gattgaagac acctaagaca ttggatacat gagcaatgga
    45721 gacgattttg gttttgtccg taatcttttt cgctgcatcc tccatatcca ggaagccttc
    45781 ttctgttagt tcgatatatt tcaagacagc acctgttcgt tttgccaact gctgccacgg
    45841 gataacatta gaatgatgtt ccatgtaaga gagcactatc tcgtctcctg ctttgatata
    45901 gcggtcgccg aaggttctgg ctacccaatt caagcttgtc gtcgtaccac gagtaaacag
    45961 cacttctgct gtttcttttg catggatgaa cgcccgtact ttttctcttg cttgttcata
    46021 atcatgggtc gcgcgttctg ctaacgtatg gacaccgcga tgaacattgg cgttatcgtg
    46081 ttcgtaatag tacgctaatg cctggagtac ttgttttggc ttttgggtcg tcgctgcatt
    46141 atccagataa actaaaggtt catcattgac gtcttgaaat aagataggaa aatcattgcg
    46201 tagcttttta gcatcgatca tccattcaac ttcccttcga ttacttcgac taattctttc
    46261 cgaacttgtt ctacagggat tgctgttaag acagaaccga ggaatcctcg aatcactaag
    46321 cgctctgctt cttctttatg caatcctcta ctcatcagat agtacatttc ttctggatca
    46381 actcgtccta cactggcagc atgtccagca gttacttcgt tttcatcgat caaaagaata
    46441 ggattcgcat caccacgtgc tttatcagaa agcatcaaga cacgactttc ttgttgggca
    46501 tctgcacctt ttgccccttt caagatgtgg ccgataccat taaaggtcaa tgttcctttt
    46561 tctcggatca ctccgtgttg caaaatatgc ccgatcgtat gaggggcttt attggttact
    46621 cgtgtatcga ttccttggat ttgtctgccc gagctgattg ccacgatttt gacttctgcg
    46681 tgtgcacctt ctcctgctaa atctgagtca aagtcagcga tcacatgacc gtcattcatg
    46741 acaccaattg cccagtcgac agaagcatct cgtaaaatgt gcccacggcg attcatataa
    46801 gaagtaatat tttctcctaa ttgatctacg gctgaatatt tgatttttga accagcttta
    46861 gcgatgactt ccacgatgat attacctgat gattttgcta cttgttcttt ggttgtctgg
    46921 aaacgttcca gataagaaaa ttcactatgt tcatctgcca cgatcaacac atgcttgaag
    46981 aagtgctcat cggatgcgcc atcttgaatg aataaggact cgagtggttc atcgatcacg
    47041 acatttttag gcacatacag gaaaataccg ctgttcataa aagctacatg gagtgcagtc
    47101 aattggtctt cgttcatgtt tactgctttt ttcatataat attcttggac tagttctgga
    47161 tattcgatca ttgcagtgaa caagtcggta aaaatgactc ctttttctgc taattccaca
    47221 ggcagttgtt caaaaatcgt ccatgaacct tgttggacaa gcacaggatt gtctttcatt
    47281 gcatcaaatg ccggaatcgt gcctgtttca ctgatggttt gtgtttcttt cacatcaaat
    47341 aacgaccagc gatggaattt cacccgatcg atatgcggca atggcagttc atctgctttc
    47401 gctagtgcgt cttgacggaa ggttctcatc caatcaggtt cgcctttact taaagaaaaa
    47461 tcatttaccg cgtcaagata atcgagccag tttttttctt tcattcttgc tcccccttac
    47521 gcttcttctt ctttatagtc gattcctaat tctttactga taccagcgta gccttctgct
    47581 tccagacgtt tagctaggtc agcattccct gtcatgacta ctcggccttc catcatgatg
    47641 tgaacgacat caggtgtgat atagttcaat aatcgttgat agtgggtgat gatcaatgca
    47701 ccgaaattgt ctccgcgcat ttcatttact cctttagcaa cgactttcaa agcatcgata
    47761 tccagacctg aatcgatttc gtctaaaatt gcaaatgtag gttctaacat caacaattgc
    47821 aggatctcat tccgtttctt ttctccgcca gaaaaacctt cgttcagata acgttcagcc
    47881 atttcttctg gcatatttag taaagccatt ttttcatcta actttttcaa aaatgccata
    47941 acagaaattt tgtcgttttc atcacgtttc gcattgatcg ctgcacgcat gaattccgca
    48001 ttcgtgatac ctggtatctc acttggatat tgcatagcta aaaataagcc aagtcgcgca
    48061 cgttcatcta cttccaagtc taaaacattt ttcccgtcaa acaaaatttc gccttctgtt
    48121 acttcatagt ttggattgcc catgatagcg gcagataagg tagattttcc tgtgccgtta
    48181 ggtcccatga tggcatggat ttctccggtt ttcattgtta ggtttacgcc tttaaggatt
    48241 tctttttcct cgatcgacac atgtagattt tttatttcta agacagacat gcattatacc
    48301 cccaaattct ctttcgttct cactcatttt agactaattg agaatgaagc gcaatgacct
    48361 tccttactaa aatcaaatat cttttattta ttattaaaca attttgattt ctaaattaga
    48421 ataggtataa aaaagatgag aaataagcca ttacagactt atttctcatc ttttttgttc
    48481 cataccttat tttgcatcag cctttactgg tttaacagag ccgccccatt tgtctgtgat
    48541 ccattcttgg acttctttgc ggtgaagcac ttcaatcagt tttttgattt tgtcactgtc
    48601 ttcatcacct ttacgagtcg caataatatt cacgtaagga gagttgtcct tttcaagcaa
    48661 tacggcatct tctaatggat tcaaccctgc gccgtaagcg aagtttgcgt tgattgcaac
    48721 taggtcacct tcatcatttt ggtacgtaga agctaaaagt gatgggtcga tcgtatggtt
    48781 gaacttcaag ttcttagggt tttcttcaat atcatcgaat gttgctgttt ccagatctac
    48841 tccatctttg actttgacta agccagcatc ttgtagaatt gtgatgatgc gtccccagtc
    48901 tgattcagaa tttgaagtaa tgattgttgc accatctttt aattcggaaa catctttgac
    48961 ttttttagag tacaagccca ttggttcgat gtggacagca ccggcattga caaagtcata
    49021 tcctttttct ttgatttctt tgttcaaata aggaatatgc tggaagtaat tggcatcaat
    49081 gtcgccttct tccaaagatt ggttcggtaa tacatagtca tcgaatttga cgatttcaag
    49141 atcgattcct tcatctttca ataatggttt cacatgttcc aaaatctctg cgtgcggtgt
    49201 cggtgaagcg ccgactttca atgtcgtgtc gtctgcttta tttgctgaat ctttgctgtc
    49261 gctgttgtta ccgcatgctg ctaatcctac agtcagcaaa agcgctgctg caaatccaaa
    49321 aattttcttt ttcatatcct acatccccct atatcctatt atcttttgtc tgttttctta
    49381 gttaagaaat caccgattcc ttgaacgata aatacgatta gtaaaataat taaagtcgca
    49441 acgaatgtca ctgttagatt accacgctgg tagccttcct gccaagcaag acctccaagt
    49501 ccacctgcgc cgatcgcgcc ggccatcgca gtaaatccga tcatcgagat cgttgtaact
    49561 gttaatccag aaatcaatgc gggaatactt tcaggtatca agaccttcca gatgatttcc
    49621 cagtaacttg ctcccattgc atctgctgct tccagtacac cagggctgac ttcacgaaaa
    49681 gcaatctcta ctagacgggc ataaaatggt gctgcggata aaacaagagc tggaatggct
    49741 gcttgcgcac ctagcatagt gcctacgatt gctttagtaa atggaatgat caaaaccatc
    49801 aggatcataa atggcgttga ccggaaaata ttactgatga tcgatacgat tccataaatg
    49861 attcgagcat acggtttgtt tttgcgtcca atagagtaaa ggatcaatcc caaaactaat
    49921 ccgatgatcg tcactaagac cattgagatt aatgtcatat atagtgtatc aaggatagcc
    49981 tgattcatcg tttccgggtt gatttgttta aaatctagat atgtttctga aaagctttta
    50041 tccacgatgg atcacctcaa ttcctactcc ttgttctttg atgaacactt ctgcttgatc
    50101 taattctttg gtttcaccag tcatcaagac agtcaatgaa ccaaaggaac cttctttcac
    50161 ttgtttgatc tttccataca caacgttgac atcgatgtta tagcgtcgaa tcgcttggga
    50221 aatgactggc tcgttggcat ttctttcgct aaaggtcaaa gttgccaaac ggccgcttgg
    50281 attttctttg atgagttcag ctaatagttc tgtcgaatca gcttctggtt cgatgtcttg
    50341 ttgtacaaag cgtttggtta cttctttttt cggttgtctg aagacagtca atacgtctcc
    50401 ttcttctacg actttcccca actccatcac agcgactttg ttgcagattt tgcggatcac
    50461 attcatttca tgtgtgatca agacgatggt caaattcaat ttcttattga tcgctaacaa
    50521 taagtctaat acttcttctg ttgtttgcgg gtccaatgcg ctagtagcct catcacaaag
    50581 caggatctca ggatcatttg ccaatgctcg tgcgataccg acgcgttgtt tctgtccgcc
    50641 ggaaagttgt gaaggatatg cttgtccgcg tccttctaac ccgactaaac gcaacagttc
    50701 ttctgcccgt tctttacgtt tgctctttgg tacgccagct aattccagcg gcagctggat
    50761 attttcaagt acagttcttg accataacag gttgaaatgc tggaagatca taccgatttt
    50821 ctttcggaat gtacgtaatt ctttgttttt taattttgta atatcttgat cgtttacgat
    50881 cacttcgccg ctcgtgggaa gctcaagtcc attcaggaga cggactaatg tactttttcc
    50941 tgcgccagaa tagccgacga ttccataaat atcccctttt tcaacgttta gtgaaacgtc
    51001 attgacggct tggatgtttc cgtttttccc attgaatgtc tttttgacat tcgttagttg
    51061 gattaatgac atgccattca catctttctc ttatgatttt tcaaacaaaa aagccttcgc
    51121 cctcttgcct taagcaaaag gacgaaagct tgaacttccg tgttaccacc ttgatttacc
    51181 gttacttcgc aataacagcc tcttccagta cttggactta gcttctttcc gtatactgtc
    51241 gcgctgtaac gggcactccc gtagtaagtt tgcttacgct cgcttactct gctctaagac
    51301 catcttccat ctcattttcg ttacccattt tcagcaaaca gggctctctt caaacgtccg
    51361 tgacatgtac tcttcttttc aaagctttct cgtttcatat aggattgctt atgattatag
    51421 caactggaag aatcagtgtc aacttttttc aagtgatttt ctgattttat aattcgtcaa
    51481 attcttttgg atcaacatct ttgaaactta agatccacgc attcatttca tctgaatcat
    51541 tcaagatatt gatatcttca ccgattttat cattaacaga agaaactgta cccgttagcg
    51601 ggctggcaaa ttcattgacc gctttttccg cttcaacttc gatcagaggt tcatcttttt
    51661 ttagcgattg tcctgctttt ggcagattgg caaacgtgat gtctccaagt tcttcttgcg
    51721 cttcatttgt caaaccaaca cagtattctt ttccattaaa taagatccaa aggttgtcct
    51781 ttttcttcaa atgcttgcct gccattttat atcagttctc cttcataaat tttttcattg
    51841 aatccaattg caacaagctg gtcctctcgt aagatcagtg gtcgcttgat cagcatacca
    51901 tctgtcgata agacttggct cgcttttgaa gccgtaaact cgcctacttg atctttcagt
    51961 ccaagttccc gatatttcat cccgcttgtg ttgaacaggc gacggattgg tacttgactc
    52021 gtttctagcc aagtctcaat cgttgaggcc ttcggtactt ctttgatcat atcaacggtt
    52081 tgataactaa cgccatgctg gtctaaccac gctttggctt ttttgcatgt tgaacatttc
    52141 gggtaccagt aaaaggtgta caattttctt acctccttga agatcttgca ggtgcttcat
    52201 tttggtatct catcgataat atcaacccga taccgatcat gttccctaaa atcgaggatc
    52261 ctccctgact aatgaaaggc aatgggatcc ctgttaaagg aagtaatccg atatttgctc
    52321 caatattctc aaagacatgg aacagcagca tcatgatcaa accgcttgcg atataggcgt
    52381 aaaattcatt attcgtatcg aaacacaccc gaatcatacg atagattaaa ataaaataca
    52441 agaaaatgac gaatgcacca ccgataaagc cgaagttttc accgataacg gtaaaaatca
    52501 tatcggactc acgaacaggc acatagatat tgcttacgtt gaatccttta ccaaacaagc
    52561 caccggtacc aattgccaaa agtccttggg ccggctgata actcattcct gacgtatcat
    52621 ggaaagggtc cagccatgaa tcaatacgtg caaactgata agatttgaat ccaactttat
    52681 aaagcaagtc tcttccgcct tcagtcgtaa ccaagaagat cgttcctgct ccaacgacta
    52741 ctgccaagat tactacaggt gcgatgatgc gccaagagat tccagacatc aggaaaatcc
    52801 cgccaaagat cgcaagaaag acaagcatcg ttccaaagtc atcttgggcc atgatcaatg
    52861 ctaaaaccgg tattgtcacg atcaagagct tcccaatcag ccaaaagtcc gatttcatcg
    52921 tccgttcttt tacttgcgta ttatgtttag ttacgatcag tgccatcatc aagatgtacg
    52981 caatcttcat caactctgct ggctggaaag taaacgcacc gaatcgaaac cagtttttag
    53041 accctgttga agcagctaaa ttctgatcat agaatttgag caagagtccc attaccacta
    53101 accccaaacc ataaaggtaa ggagtcagtt tccagatcca tttggaattc aaatgcatga
    53161 tgacaacgat tgctgccgct ccaactaaat accagacaac ttgcatccct acgcctttca
    53221 agactgctgg attgttcgga tcatggctaa gtgctacata taatgagagc agaccaatca
    53281 aacataacag aaagactggt aaaatcacgc cataatcaat acgattatcg ttcgaagctt
    53341 ttaatttttt ttccatgttt catccctttt cttaaaaacc atactgctga ttattatagc
    53401 ggtccagtta gataatggaa agtctttcca tagacatttg ggaaaaatta actttccgtt
    53461 tgtgcagttg cttgagcaaa tgcagtacaa aagcctactg cttatcttta gtgtatcata
    53521 gaactttttt tgatacatgc aattatctac cagctgagat tctcctcgct gtgagaagtt
    53581 tctgtgcttt cattgtatcg aaggatctct tcggcaatct ctagcagctg atcagaaacc
    53641 agattggttc gttggatgaa ataaaaatat cgatggaaat tcaacggagt cattggcaca
    53701 ttttctggaa cagagcgctt agacaaaatc gagcgaccga ttccttcctt cagcaacgca
    53761 acaattacat cgttactttt cacgatcatc ttcttatctt ggatattcat ctcttcaaaa
    53821 taacgctgcg tgtaatgaaa aacaccagag tttgcttctc ttacaagcca caatttgctt
    53881 tttgggtccc cagcgatgac tagctggtct tccatcaaag agtatctttg tacgccggaa
    53941 gtctctaatg gcttttcgat aaaaccaaaa tcggattcat gtttcgctac ttgttctaat
    54001 gattcatgag aattcgtgat ttcgatctca aaatgcactt taggaaacct tttgatcaga
    54061 tgtttcatca actgaggcaa aagatagaca gcaaaggtgt gggaagcaac gatccgacat
    54121 acttcttctt cattttgttc attgagcaat ttttgctgag tttcttccca ttcttctaac
    54181 aaaaccagtg tccgttcata taataagtcg gcttgcttag ttggaagcat ctcttttctt
    54241 ccgttacgtt gaaaaagatc gatattcaat tcccgctcca actgtttgat ttgattcgag
    54301 actgctggtt gagataaaaa cagtacttct gctgctctgg aaaaatttct tgtttcatat
    54361 actgctcgaa aagtcgttaa taacttaaac atggcaccac ttccataact ttttatgata
    54421 attactataa aaactatttg atttaaatat acaaaaaacc atctataata gaaatgtaca
    54481 acaaaacagt tgctgatcca actagtaact tagatgaaga caatcagctg tctgcttgct
    54541 cgtaatcata cattcctatt cattttacaa tgtattcgtt catcttggta ctaaaatcta
    54601 ttaaataaaa aggagaatat tatatatgaa gaactttttg aaaattattc cgatcccgat
    54661 ctgcgggttg atccttgggt tagcttccct tggaaatctg tttaaagact atcaccatgt
    54721 tgctttaggc aatctaactg gcggtatctc tatgattctt atgatattca tttttgttaa
    54781 attgtttatc ttattcgaac acacgaaaca aacgttgcat gatccaatca tcgcctccgt
    54841 ttcaccaact tttacgatgt cactcatggt tatttgtact tattttgttt catatgaaag
    54901 tattgcacca attgtcaaat tcatttggct gtgcgctgtt atcttccaag tgatccttgt
    54961 cttttatttc aattatcacc atgttatcaa agctgatctt tcaattgaag caatttatcc
    55021 aagctggttt atcatttacg ttggatttgg ggtcatcact gtaactgccg ggaacttctt
    55081 cccacttatt ggaaaaattt tcttttggat ttcattgatc tgctatgcgt tcttattgcc
    55141 gatcatcatc aaacgtgtct ttttcgtaag aaatatggct catccgacac gtccgctgat
    55201 tacgatcgtt gcagcacctg gttctctttg cctaacaggt tatcttaaaa actttgctga
    55261 acctaatttt tatcttgtcg tagcattgtt cgctttatct caagtattgt atttcgttgt
    55321 gctgtctatg cttccacgtt tgttcaagct tcgtttttat ccaagctatg cagcctttac
    55381 attcccgctt gttatctctg caacagctat atttactact gttcattact tcaacagttt
    55441 aggtatctat agtagcgtat tggatttctt aaaagttgcg gaatgtctgt ttgcaagtgt
    55501 agttgtcttc tacgttcttg ctcattacgt acgtttctta attaaagaac atagcaaaca
    55561 tggaaaattt gtttctaaaa aaatcgctta ataatcgatt caataggttg tgaaaaagct
    55621 gttctgcatc aagtaataag aacggccaaa caaaaacagc tgttcatgtt tttgtactgg
    55681 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taccaagagg tcgtaa
//
LOCUS       DACIFS010000010        55197 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.010, whole genome shotgun sequence.
ACCESSION   DACIFS010000010 DACIFS010000000
VERSION     DACIFS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.010"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            368..1471
                     /locus_tag="IYA83_000608"
     CDS             368..1471
                     /locus_tag="IYA83_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ8586065.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1755..2435
                     /locus_tag="IYA83_000609"
     CDS             1755..2435
                     /locus_tag="IYA83_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ8586066.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2525..3559
                     /locus_tag="IYA83_000610"
     CDS             2525..3559
                     /locus_tag="IYA83_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ8586067.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3573..3908
                     /locus_tag="IYA83_000611"
     CDS             3573..3908
                     /locus_tag="IYA83_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586068.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3892..6372
                     /locus_tag="IYA83_000612"
     CDS             3892..6372
                     /locus_tag="IYA83_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586069.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6428..7987
                     /locus_tag="IYA83_000613"
     CDS             6428..7987
                     /locus_tag="IYA83_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586070.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7984..9348
                     /locus_tag="IYA83_000614"
     CDS             7984..9348
                     /locus_tag="IYA83_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ8586071.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9519..10589
                     /locus_tag="IYA83_000615"
     CDS             9519..10589
                     /locus_tag="IYA83_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586072.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10604..11272
                     /locus_tag="IYA83_000616"
     CDS             10604..11272
                     /locus_tag="IYA83_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586073.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11292..11870
                     /locus_tag="IYA83_000617"
     CDS             11292..11870
                     /locus_tag="IYA83_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8586074.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12273..13184
                     /locus_tag="IYA83_000618"
     CDS             12273..13184
                     /locus_tag="IYA83_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ8586075.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13440..14357)
                     /locus_tag="IYA83_000619"
     CDS             complement(13440..14357)
                     /locus_tag="IYA83_000619"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ8586076.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14559..14996
                     /gene="zapA"
                     /locus_tag="IYA83_000620"
     CDS             14559..14996
                     /gene="zapA"
                     /locus_tag="IYA83_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ8586077.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15054..15602
                     /locus_tag="IYA83_000621"
     CDS             15054..15602
                     /locus_tag="IYA83_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ8586078.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15717..18077
                     /locus_tag="IYA83_000622"
     CDS             15717..18077
                     /locus_tag="IYA83_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ8586079.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18164..18478
                     /gene="trxA"
                     /locus_tag="IYA83_000623"
     CDS             18164..18478
                     /gene="trxA"
                     /locus_tag="IYA83_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ8586080.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18660..20438
                     /gene="uvrC"
                     /locus_tag="IYA83_000624"
     CDS             18660..20438
                     /gene="uvrC"
                     /locus_tag="IYA83_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ8586081.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20583..21482
                     /locus_tag="IYA83_000625"
     CDS             20583..21482
                     /locus_tag="IYA83_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586082.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21475..22737
                     /locus_tag="IYA83_000626"
     CDS             21475..22737
                     /locus_tag="IYA83_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586083.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22900..24798
                     /locus_tag="IYA83_000627"
     CDS             22900..24798
                     /locus_tag="IYA83_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ8586084.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25097..25816)
                     /locus_tag="IYA83_000628"
     CDS             complement(25097..25816)
                     /locus_tag="IYA83_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ8586085.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26153..26485
                     /locus_tag="IYA83_000629"
     CDS             26153..26485
                     /locus_tag="IYA83_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586086.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26472..28475
                     /locus_tag="IYA83_000630"
     CDS             26472..28475
                     /locus_tag="IYA83_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ8586087.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28494..28964
                     /locus_tag="IYA83_000631"
     CDS             28494..28964
                     /locus_tag="IYA83_000631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ8586088.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28985..29569
                     /locus_tag="IYA83_000632"
     CDS             28985..29569
                     /locus_tag="IYA83_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ8586089.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29584..30570
                     /locus_tag="IYA83_000633"
     CDS             29584..30570
                     /locus_tag="IYA83_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ8586090.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30560..30871
                     /locus_tag="IYA83_000634"
     CDS             30560..30871
                     /locus_tag="IYA83_000634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ8586091.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30893..32674
                     /locus_tag="IYA83_000635"
     CDS             30893..32674
                     /locus_tag="IYA83_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ8586092.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMCG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32667..34043
                     /locus_tag="IYA83_000636"
     CDS             32667..34043
                     /locus_tag="IYA83_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ8586093.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34050..34685
                     /locus_tag="IYA83_000637"
     CDS             34050..34685
                     /locus_tag="IYA83_000637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ8586094.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34715..36070
                     /locus_tag="IYA83_000638"
     CDS             34715..36070
                     /locus_tag="IYA83_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ8586095.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36262..37656)
                     /locus_tag="IYA83_000639"
     CDS             complement(36262..37656)
                     /locus_tag="IYA83_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ8586096.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37748..38719
                     /locus_tag="IYA83_000640"
     CDS             37748..38719
                     /locus_tag="IYA83_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ8586097.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38716..39498
                     /locus_tag="IYA83_000641"
     CDS             38716..39498
                     /locus_tag="IYA83_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ8586098.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39667..40038)
                     /locus_tag="IYA83_000642"
     CDS             complement(39667..40038)
                     /locus_tag="IYA83_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ8586099.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40172..40615
                     /locus_tag="IYA83_000643"
     CDS             40172..40615
                     /locus_tag="IYA83_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ8586100.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="IYA83_000644"
     CDS             complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="IYA83_000644"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ8586101.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41486..42853)
                     /locus_tag="IYA83_000645"
     CDS             complement(41486..42853)
                     /locus_tag="IYA83_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586102.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42837..43667)
                     /locus_tag="IYA83_000646"
     CDS             complement(42837..43667)
                     /locus_tag="IYA83_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ8586103.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43830..44267
                     /locus_tag="IYA83_000647"
     CDS             43830..44267
                     /locus_tag="IYA83_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ8586104.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44343..44963
                     /locus_tag="IYA83_000648"
     CDS             44343..44963
                     /locus_tag="IYA83_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ8586105.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45152..45658
                     /locus_tag="IYA83_000649"
     CDS             45152..45658
                     /locus_tag="IYA83_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ8586106.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45675..46574
                     /locus_tag="IYA83_000650"
     CDS             45675..46574
                     /locus_tag="IYA83_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ8586107.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46661..47545
                     /locus_tag="IYA83_000651"
     CDS             46661..47545
                     /locus_tag="IYA83_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ8586108.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47737..47808)
                     /locus_tag="IYA83_000652"
     tRNA            complement(47737..47808)
                     /locus_tag="IYA83_000652"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47773..47775),aa:Arg,seq:ccg)
     gene            47868..49202
                     /gene="rpoN"
                     /locus_tag="IYA83_000653"
     CDS             47868..49202
                     /gene="rpoN"
                     /locus_tag="IYA83_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ8586109.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49405..50442
                     /locus_tag="IYA83_000654"
     CDS             49405..50442
                     /locus_tag="IYA83_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ8586110.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50483..51484
                     /gene="gap"
                     /locus_tag="IYA83_000655"
     CDS             50483..51484
                     /gene="gap"
                     /locus_tag="IYA83_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ8586111.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51594..52784
                     /locus_tag="IYA83_000656"
     CDS             51594..52784
                     /locus_tag="IYA83_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ8586112.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52888..53643
                     /locus_tag="IYA83_000657"
     CDS             52888..53643
                     /locus_tag="IYA83_000657"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ8586113.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53759..55057
                     /gene="eno"
                     /locus_tag="IYA83_000658"
     CDS             53759..55057
                     /gene="eno"
                     /locus_tag="IYA83_000658"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ8586114.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18304 a         9608 c        11686 g        15599 t
ORIGIN      
        1 ataccgaggg tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag
       61 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac
      121 ttaataccga gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta
      181 cttttgtcaa agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt
      241 agtgacatct ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag
      301 taatataaaa agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg
      361 cattgagatg gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa
      421 tatggaagat ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat
      481 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg
      541 tttgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
      601 cgaagaaatg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca
      661 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
      721 aagttcttac gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc
      781 gcaatggttt caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt
      841 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
      901 ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg ccaatgccga tcgttcaggc
      961 ttatcaatca ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact
     1021 tagtccaaaa gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg
     1081 tgttcaatca gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt
     1141 atctaaccat ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata
     1201 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg
     1261 acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt
     1321 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
     1381 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
     1441 aataaaattg cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc
     1501 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
     1561 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
     1621 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
     1681 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
     1741 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
     1801 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
     1861 cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag
     1921 tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg
     1981 tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
     2041 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
     2101 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
     2161 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
     2221 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
     2281 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
     2341 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
     2401 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
     2461 cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg
     2521 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
     2581 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
     2641 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
     2701 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
     2761 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
     2821 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
     2881 ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc
     2941 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
     3001 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
     3061 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
     3121 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
     3181 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
     3241 tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
     3301 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
     3361 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
     3421 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
     3481 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
     3541 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
     3601 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
     3661 caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg
     3721 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
     3781 tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat
     3841 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
     3901 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
     3961 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
     4021 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
     4081 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
     4141 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
     4201 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
     4261 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
     4321 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
     4381 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
     4441 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
     4501 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
     4561 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
     4621 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
     4681 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
     4741 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
     4801 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
     4861 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
     4921 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
     4981 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
     5041 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
     5101 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
     5161 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
     5221 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
     5281 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
     5341 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
     5401 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
     5461 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
     5521 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
     5581 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
     5641 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
     5701 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
     5761 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
     5821 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
     5881 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
     5941 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
     6001 cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg
     6061 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
     6121 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
     6181 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
     6241 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
     6301 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
     6361 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
     6421 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
     6481 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
     6541 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
     6601 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
     6661 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
     6721 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
     6781 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
     6841 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
     6901 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
     6961 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
     7021 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
     7081 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
     7141 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
     7201 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
     7261 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
     7321 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
     7381 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
     7441 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
     7501 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
     7561 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
     7621 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
     7681 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
     7741 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
     7801 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
     7861 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
     7921 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
     7981 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
     8041 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
     8101 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
     8161 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
     8221 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
     8281 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
     8341 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
     8401 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
     8461 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
     8521 aatgttttaa caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
     8581 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
     8641 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
     8701 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
     8761 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
     8821 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
     8881 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
     8941 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
     9001 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
     9061 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
     9121 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
     9181 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
     9241 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
     9301 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
     9361 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
     9421 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
     9481 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
     9541 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
     9601 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
     9661 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
     9721 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
     9781 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
     9841 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
     9901 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
     9961 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    10021 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
    10081 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    10141 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    10201 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    10261 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    10321 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    10381 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    10441 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    10501 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    10561 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
    10621 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    10681 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    10741 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    10801 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
    10861 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    10921 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
    10981 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    11041 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    11101 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    11161 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    11221 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    11281 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    11341 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
    11401 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
    11461 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    11521 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    11581 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    11641 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    11701 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    11761 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    11821 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    11881 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
    11941 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
    12001 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
    12061 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
    12121 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
    12181 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
    12241 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
    12301 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
    12361 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
    12421 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
    12481 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
    12541 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
    12601 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
    12661 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
    12721 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
    12781 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
    12841 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
    12901 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
    12961 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
    13021 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
    13081 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
    13141 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
    13201 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
    13261 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
    13321 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
    13381 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
    13441 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
    13501 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
    13561 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
    13621 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
    13681 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
    13741 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
    13801 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
    13861 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
    13921 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    13981 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    14041 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    14101 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    14161 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    14221 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    14281 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    14341 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    14401 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    14461 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    14521 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    14581 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    14641 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    14701 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    14761 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    14821 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    14881 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    14941 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    15001 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    15061 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    15121 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    15181 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    15241 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    15301 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    15361 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    15421 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    15481 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
    15541 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    15601 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc
    15661 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    15721 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    15781 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa
    15841 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    15901 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag
    15961 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    16021 tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt
    16081 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    16141 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    16201 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    16261 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    16321 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    16381 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    16441 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    16501 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    16561 cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg
    16621 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    16681 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    16741 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    16801 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    16861 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    16921 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    16981 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    17041 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata
    17101 cgattaatgc cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga
    17161 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    17221 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    17281 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    17341 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    17401 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg
    17461 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    17521 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    17581 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    17641 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    17701 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    17761 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    17821 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    17881 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    17941 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    18001 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    18061 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    18121 aatgctatac taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    18181 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    18241 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    18301 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    18361 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    18421 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    18481 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    18541 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    18601 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    18661 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    18721 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    18781 tccgctcata ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg
    18841 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    18901 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    18961 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag
    19021 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    19081 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    19141 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    19201 atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt
    19261 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    19321 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    19381 ctgatttact ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc
    19441 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    19501 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    19561 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    19621 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    19681 cgaataagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    19741 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    19801 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    19861 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    19921 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga
    19981 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    20041 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    20101 caaaaaacga taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac
    20161 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    20221 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    20281 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    20341 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    20401 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    20461 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    20521 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    20581 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    20641 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    20701 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    20761 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    20821 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    20881 ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa
    20941 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    21001 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    21061 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    21121 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    21181 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    21241 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    21301 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    21361 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    21421 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    21481 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    21541 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    21601 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    21661 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    21721 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    21781 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    21841 atacaacaac aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca
    21901 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    21961 gattcaaaag gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt
    22021 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    22081 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    22141 caaaagcatt tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca
    22201 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    22261 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    22321 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    22381 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    22441 ggatatatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    22501 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    22561 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    22621 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta
    22681 tggagttcat tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat
    22741 atagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    22801 ctatttctat ttttttatta tattggatat atatattcat tgaaaataga gcataacaac
    22861 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
    22921 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
    22981 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
    23041 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
    23101 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
    23161 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
    23221 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
    23281 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
    23341 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
    23401 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
    23461 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
    23521 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
    23581 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
    23641 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
    23701 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
    23761 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
    23821 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
    23881 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
    23941 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
    24001 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
    24061 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
    24121 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
    24181 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
    24241 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
    24301 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
    24361 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
    24421 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
    24481 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
    24541 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
    24601 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
    24661 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
    24721 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
    24781 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
    24841 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
    24901 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
    24961 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
    25021 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    25081 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
    25141 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
    25201 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
    25261 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
    25321 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
    25381 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
    25441 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
    25501 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
    25561 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
    25621 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
    25681 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
    25741 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
    25801 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
    25861 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    25921 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    25981 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    26041 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    26101 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    26161 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
    26221 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
    26281 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
    26341 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    26401 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
    26461 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    26521 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
    26581 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
    26641 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
    26701 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    26761 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
    26821 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
    26881 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    26941 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    27001 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    27061 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
    27121 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
    27181 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
    27241 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    27301 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
    27361 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
    27421 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    27481 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
    27541 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
    27601 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
    27661 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    27721 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    27781 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    27841 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    27901 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    27961 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    28021 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    28081 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    28141 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    28201 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    28261 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    28321 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    28381 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    28441 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    28501 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    28561 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    28621 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    28681 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    28741 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    28801 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    28861 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    28921 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    28981 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    29041 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    29101 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    29161 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    29221 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    29281 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    29341 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    29401 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    29461 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    29521 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    29581 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    29641 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    29701 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    29761 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    29821 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    29881 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    29941 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    30001 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    30061 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    30121 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    30181 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    30241 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    30301 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    30361 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    30421 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    30481 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    30541 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    30601 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    30661 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    30721 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    30781 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    30841 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    30901 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    30961 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgtg
    31021 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    31081 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    31141 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    31201 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    31261 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    31321 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    31381 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    31441 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    31501 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    31561 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    31621 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    31681 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    31741 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    31801 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    31861 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    31921 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    31981 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    32041 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    32101 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    32161 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    32221 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    32281 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    32341 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    32401 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    32461 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    32521 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    32581 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    32641 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    32701 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    32761 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    32821 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    32881 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    32941 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    33001 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    33061 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    33121 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    33181 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    33241 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    33301 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    33361 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    33421 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    33481 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    33541 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    33601 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    33661 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    33721 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    33781 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    33841 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    33901 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    33961 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    34021 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    34081 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    34141 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    34201 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    34261 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    34321 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    34381 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    34441 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    34501 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    34561 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    34621 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    34681 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    34741 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    34801 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    34861 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    34921 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    34981 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    35041 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    35101 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    35161 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    35221 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    35281 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    35341 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    35401 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    35461 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    35521 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    35581 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    35641 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    35701 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    35761 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    35821 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    35881 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    35941 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    36001 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    36061 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    36121 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    36181 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    36241 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    36301 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    36361 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    36421 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    36481 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    36541 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    36601 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    36661 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    36721 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    36781 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    36841 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    36901 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    36961 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    37021 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    37081 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    37141 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    37201 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    37261 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    37321 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    37381 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    37441 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    37501 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    37561 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    37621 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    37681 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    37741 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    37801 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    37861 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    37921 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    37981 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    38041 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    38101 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    38161 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    38221 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    38281 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    38341 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    38401 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    38461 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    38521 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    38581 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    38641 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    38701 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    38761 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    38821 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    38881 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    38941 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    39001 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    39061 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    39121 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    39181 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    39241 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    39301 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    39361 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    39421 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    39481 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    39541 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    39601 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    39661 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    39721 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    39781 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    39841 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    39901 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    39961 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    40021 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    40081 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    40141 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    40201 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    40261 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    40321 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    40381 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    40441 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    40501 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    40561 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    40621 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    40681 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    40741 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    40801 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    40861 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    40921 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    40981 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    41041 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    41101 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    41161 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    41221 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    41281 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    41341 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    41401 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    41461 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    41521 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    41581 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    41641 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    41701 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    41761 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    41821 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    41881 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    41941 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    42001 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    42061 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    42121 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    42181 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    42241 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    42301 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    42361 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    42421 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    42481 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    42541 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    42601 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    42661 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    42721 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    42781 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    42841 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    42901 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    42961 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    43021 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    43081 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    43141 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    43201 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    43261 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    43321 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    43381 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    43441 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    43501 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    43561 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    43621 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    43681 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    43741 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    43801 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    43861 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    43921 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    43981 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    44041 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    44101 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    44161 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    44221 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    44281 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    44341 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    44401 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    44461 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    44521 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    44581 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    44641 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    44701 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    44761 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    44821 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    44881 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    44941 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    45001 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    45061 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    45121 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    45181 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    45241 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    45301 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    45361 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    45421 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    45481 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    45541 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    45601 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    45661 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    45721 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    45781 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    45841 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    45901 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    45961 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    46021 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    46081 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    46141 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    46201 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    46261 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    46321 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    46381 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    46441 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    46501 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    46561 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    46621 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    46681 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    46741 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    46801 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    46861 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    46921 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    46981 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    47041 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    47101 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    47161 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    47221 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    47281 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    47341 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    47401 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    47461 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    47521 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    47581 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    47641 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    47701 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    47761 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    47821 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    47881 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    47941 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    48001 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    48061 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    48121 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    48181 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    48241 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    48301 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    48361 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    48421 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    48481 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    48541 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    48601 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    48661 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    48721 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    48781 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    48841 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    48901 gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat
    48961 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    49021 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg
    49081 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    49141 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    49201 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    49261 agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    49321 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    49381 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    49441 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    49501 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    49561 acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    49621 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    49681 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    49741 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    49801 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    49861 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    49921 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    49981 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    50041 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    50101 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    50161 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    50221 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    50281 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    50341 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    50401 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    50461 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    50521 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    50581 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    50641 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    50701 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    50761 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    50821 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    50881 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    50941 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    51001 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    51061 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    51121 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    51181 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    51241 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    51301 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    51361 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    51421 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    51481 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    51541 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    51601 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    51661 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    51721 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    51781 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    51841 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    51901 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    51961 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    52021 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    52081 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    52141 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    52201 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    52261 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    52321 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    52381 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    52441 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    52501 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    52561 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    52621 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    52681 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    52741 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    52801 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    52861 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    52921 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    52981 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    53041 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    53101 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    53161 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    53221 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    53281 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    53341 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    53401 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    53461 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    53521 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    53581 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    53641 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    53701 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    53761 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    53821 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    53881 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    53941 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    54001 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    54061 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    54121 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    54181 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    54241 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    54301 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    54361 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    54421 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    54481 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    54541 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    54601 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    54661 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    54721 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    54781 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    54841 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    54901 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    54961 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    55021 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    55081 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    55141 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgttt
//
LOCUS       DACIFS010000011        54992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.011, whole genome shotgun sequence.
ACCESSION   DACIFS010000011 DACIFS010000000
VERSION     DACIFS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.011"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            4..1086
                     /locus_tag="IYA83_000659"
     CDS             4..1086
                     /locus_tag="IYA83_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8586115.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="IYA83_000660"
     CDS             complement(1304..2446)
                     /locus_tag="IYA83_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8586116.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="IYA83_000661"
     CDS             complement(2451..2636)
                     /locus_tag="IYA83_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ8586117.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="IYA83_000662"
     CDS             complement(2990..3235)
                     /locus_tag="IYA83_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586118.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="IYA83_000663"
     CDS             complement(3229..3630)
                     /locus_tag="IYA83_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ8586119.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYA83_000664"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="IYA83_000664"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ8586120.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="IYA83_000665"
     CDS             complement(5648..7414)
                     /locus_tag="IYA83_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ8586121.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7427..8221)
                     /locus_tag="IYA83_000666"
     CDS             complement(7427..8221)
                     /locus_tag="IYA83_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ8586122.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8231..9607)
                     /locus_tag="IYA83_000667"
     CDS             complement(8231..9607)
                     /locus_tag="IYA83_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8586123.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYA83_000668"
     CDS             complement(9727..10374)
                     /gene="eda"
                     /locus_tag="IYA83_000668"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ8586124.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYA83_000669"
     CDS             complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="IYA83_000669"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ8586125.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYA83_000670"
     CDS             complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="IYA83_000670"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ8586126.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13041..14672)
                     /locus_tag="IYA83_000671"
     CDS             complement(13041..14672)
                     /locus_tag="IYA83_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ8586127.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14976..15647
                     /locus_tag="IYA83_000672"
     CDS             14976..15647
                     /locus_tag="IYA83_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8586128.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16372..16707)
                     /locus_tag="IYA83_000673"
                     /pseudo
     CDS             complement(16372..16707)
                     /locus_tag="IYA83_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16804..17256)
                     /locus_tag="IYA83_000674"
     CDS             complement(16804..17256)
                     /locus_tag="IYA83_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586129.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17263..17811)
                     /locus_tag="IYA83_000675"
     CDS             complement(17263..17811)
                     /locus_tag="IYA83_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8586130.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17856..18188)
                     /locus_tag="IYA83_000676"
     CDS             complement(17856..18188)
                     /locus_tag="IYA83_000676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586131.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18202..18432)
                     /locus_tag="IYA83_000677"
     CDS             complement(18202..18432)
                     /locus_tag="IYA83_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586132.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18654..19196)
                     /locus_tag="IYA83_000678"
     CDS             complement(18654..19196)
                     /locus_tag="IYA83_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586133.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19217..20308)
                     /locus_tag="IYA83_000679"
     CDS             complement(19217..20308)
                     /locus_tag="IYA83_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ8586134.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20316..22526)
                     /locus_tag="IYA83_000680"
     CDS             complement(20316..22526)
                     /locus_tag="IYA83_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586135.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22542..25070)
                     /locus_tag="IYA83_000681"
     CDS             complement(22542..25070)
                     /locus_tag="IYA83_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ8586136.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25122..25532)
                     /locus_tag="IYA83_000682"
     CDS             complement(25122..25532)
                     /locus_tag="IYA83_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586137.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25545..25763)
                     /locus_tag="IYA83_000683"
     CDS             complement(25545..25763)
                     /locus_tag="IYA83_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586138.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25760..26752)
                     /locus_tag="IYA83_000684"
     CDS             complement(25760..26752)
                     /locus_tag="IYA83_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8586139.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26831..27742)
                     /locus_tag="IYA83_000685"
     CDS             complement(26831..27742)
                     /locus_tag="IYA83_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ8586140.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27761..28105)
                     /locus_tag="IYA83_000686"
     CDS             complement(27761..28105)
                     /locus_tag="IYA83_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586141.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28125..28681)
                     /locus_tag="IYA83_000687"
                     /pseudo
     CDS             complement(28125..28681)
                     /locus_tag="IYA83_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28762..29262)
                     /locus_tag="IYA83_000688"
     CDS             complement(28762..29262)
                     /locus_tag="IYA83_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586142.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29263..29808)
                     /locus_tag="IYA83_000689"
     CDS             complement(29263..29808)
                     /locus_tag="IYA83_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586143.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29805..31061)
                     /locus_tag="IYA83_000690"
     CDS             complement(29805..31061)
                     /locus_tag="IYA83_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ8586144.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31422..31733)
                     /locus_tag="IYA83_000691"
     CDS             complement(31422..31733)
                     /locus_tag="IYA83_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586145.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31756..33066)
                     /locus_tag="IYA83_000692"
     CDS             complement(31756..33066)
                     /locus_tag="IYA83_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ8586146.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33680..34132)
                     /locus_tag="IYA83_000693"
     CDS             complement(33680..34132)
                     /locus_tag="IYA83_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586147.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34134..34499)
                     /locus_tag="IYA83_000694"
     CDS             complement(34134..34499)
                     /locus_tag="IYA83_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586148.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34598..38881)
                     /locus_tag="IYA83_000695"
     CDS             complement(34598..38881)
                     /locus_tag="IYA83_000695"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAQ8586149.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKAIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39028..39267)
                     /locus_tag="IYA83_000696"
     CDS             complement(39028..39267)
                     /locus_tag="IYA83_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586150.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39357..39629)
                     /locus_tag="IYA83_000697"
     CDS             complement(39357..39629)
                     /locus_tag="IYA83_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586151.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39712..39963)
                     /locus_tag="IYA83_000698"
     CDS             complement(39712..39963)
                     /locus_tag="IYA83_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586152.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40959..41870)
                     /locus_tag="IYA83_000699"
     CDS             complement(40959..41870)
                     /locus_tag="IYA83_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586153.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="IYA83_000700"
     CDS             complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="IYA83_000700"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ8586154.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44048..45268
                     /locus_tag="IYA83_000701"
     CDS             44048..45268
                     /locus_tag="IYA83_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586155.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45288..45782
                     /locus_tag="IYA83_000702"
     CDS             45288..45782
                     /locus_tag="IYA83_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ8586156.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45775..46446
                     /locus_tag="IYA83_000703"
     CDS             45775..46446
                     /locus_tag="IYA83_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586157.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="IYA83_000704"
     CDS             complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="IYA83_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ8586158.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49457..50503)
                     /locus_tag="IYA83_000705"
     CDS             complement(49457..50503)
                     /locus_tag="IYA83_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ8586159.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50514..50786)
                     /locus_tag="IYA83_000706"
     CDS             complement(50514..50786)
                     /locus_tag="IYA83_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ8586160.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50821..51729)
                     /locus_tag="IYA83_000707"
     CDS             complement(50821..51729)
                     /locus_tag="IYA83_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ8586161.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="IYA83_000708"
     CDS             complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="IYA83_000708"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ8586162.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53108..54229)
                     /locus_tag="IYA83_000709"
     CDS             complement(53108..54229)
                     /locus_tag="IYA83_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ8586163.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54226..54927)
                     /locus_tag="IYA83_000710"
     CDS             complement(54226..54927)
                     /locus_tag="IYA83_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ8586164.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT        16578 a        11367 c         8238 g        18809 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac
     7501 atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa
     7561 taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa
     7621 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt
     7681 tttttccttt atcttgacaa cacagttcat caactaatga taataattca tgtctttccc
     7741 attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
     7801 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac
     7861 taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
     7921 aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt
     7981 gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa
     8041 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
     8101 tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt
     8161 tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca
     8221 tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt
     8281 tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa
     8341 ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag
     8401 agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta
     8461 tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta
     8521 cttcttgatc atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca
     8581 aacccatata gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca
     8641 aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt
     8701 ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag
     8761 gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat
     8821 aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta
     8881 tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt
     8941 attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa
     9001 taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta
     9061 tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca
     9121 aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa
     9181 tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac
     9241 tcccccataa tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa
     9301 tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa
     9361 tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag
     9421 ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc
     9481 cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag
     9541 taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc
     9601 tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac
     9661 tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt
     9721 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctactcgact
     9781 tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc
     9841 aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat
     9901 tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga
     9961 acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat
    10021 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg
    10081 atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa
    10141 cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc
    10201 aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat
    10261 tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac
    10321 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
    10381 ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag aataaattta
    10441 agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac
    10501 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
    10561 cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg
    10621 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
    10681 ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
    10741 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
    10801 gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca
    10861 ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
    10921 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
    10981 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
    11041 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
    11101 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
    11161 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc
    11221 aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt
    11281 aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta
    11341 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct
    11401 gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg
    11461 cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc
    11521 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
    11581 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
    11641 tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta
    11701 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
    11761 ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt
    11821 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
    11881 aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa
    11941 ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
    12001 taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc
    12061 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
    12121 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa
    12181 atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
    12241 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
    12301 tccagtacat aatgtaatac cattagaagg agaatccact acattacaca accaatctaa
    12361 atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg
    12421 gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
    12481 tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt
    12541 aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
    12601 tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt
    12661 agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat
    12721 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
    12781 attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac
    12841 actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat
    12901 tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt
    12961 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
    13021 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
    13081 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct
    13141 accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc
    13201 gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc
    13261 aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc
    13321 agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
    13381 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
    13441 gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta
    13501 attacttcat caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa
    13561 ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct
    13621 gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc
    13681 aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc
    13741 acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa
    13801 tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata
    13861 tctaaatcct cgagccccat catcaataga tcttccttta cattctctga aggatttggc
    13921 gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
    13981 ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta
    14041 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac
    14101 tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga
    14161 ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct
    14221 tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca
    14281 aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata taaactatca
    14341 gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa
    14401 atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac
    14461 acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
    14521 aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct
    14581 tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg
    14641 atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag
    14701 ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata
    14761 aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac
    14821 tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt
    14881 atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa
    14941 atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa
    15001 atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa
    15061 aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag
    15121 cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata
    15181 tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac
    15241 gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa
    15301 ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata
    15361 atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat
    15421 ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag
    15481 gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac
    15541 aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc
    15601 aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt
    15661 ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata
    15721 tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt
    15781 atgactgctg ataacttttg atattaaact agtatctcca atacaaataa aaaatagata
    15841 tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc
    15901 ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact
    15961 ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg
    16021 ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg cttttttcaa
    16081 aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac
    16141 atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact tataagaaat
    16201 tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca
    16261 gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc
    16321 cgattactta tattctacgt atagttttag tattcttatt ttttatcacc agtaaccaat
    16381 tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt caggcttagg
    16441 aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag aaataccact
    16501 gcctatcttt tttacaaata tttcatttgg tgtacagact aataaaacaa tatttgtaat
    16561 caatccttgc actaaagaat taaaaggaag actatttaaa acagggtgtg cataactata
    16621 acatgtgaag tagttatttt gaactttaac ttttaaaaac tttaaaaata atcttatttg
    16681 ttcttctaaa caatcatcta tactcatata atcactccac taattaatct ccccaaaaag
    16741 acacagcatt aagagatata ccaacacaac agtgcaaata actctacatc ttccttcgca
    16801 accttatttc aataaaccat aaaaattatt ttttaataga aactgaaaat taaaactaga
    16861 aaaagtagtt ttaccgaatg taagagcatc atcaccatca atatagaaat acattctttt
    16921 ccagttacat ttaattttta aacaaaattc tccccaaaaa ggaacttttt ctagtgaaaa
    16981 cttggtaatc tctttgtaat taattcttgt taaagaataa ttaaacactt catttttact
    17041 tgttattttt tcattgacta taactagttc atctggtaaa aaaattaacc aacaaacatc
    17101 gacaaacagt aaatgatata atccatttcc acctccaagc atattcaata ctggtgaaca
    17161 tgccttagaa acacgaatat agttatggtt taaatagata ttttctttaa acattgcttg
    17221 tttaaaagcc atatgctctt gttctaaact tttcataaac ttttactcta atgcacagct
    17281 agcagcagct gctgcaattc ctgctacaat atttacctta gctaactctt tagctgcagc
    17341 ttttcctaat tgtttttctg caatttttaa taataaatcc gcagcttttt caaaattaaa
    17401 agcttttaat gcttcaataa aatctccctg cgtacctata gcccaagcca ctcctcctcc
    17461 aggaagagaa ttaatggcta cacatccagc atatcgtttc caatcaactt tctccattct
    17521 aggaacaata atatgttcat aaaagactct tgctgctctt ccttgttcgt tatcttgact
    17581 gattcgttca cgaatagcac caaaatcctt tatatggtat acaccatgtt cgtctaaaaa
    17641 accaactgta tcaaaataaa aagttaattc atctactaat ttattcttta tttctaaatc
    17701 aactgtattc actttttctg tggcatacac acttgttcca gtactaacaa aagtacttaa
    17761 acctaataag aaaacaccta aaacaccaaa tattttaatt attcttttca taatttctcc
    17821 tccataaaaa ataagttact ttttaaatat cttgtttatg aattcattcc ccaaaggcct
    17881 tttaagattg atacagcagt ccgttcactt cttttagact tgtcatacaa gtcttttaaa
    17941 tgatcccata tcagactagt ttgtctatct agcttcaatc cattaaacgc taatgaacgg
    18001 ttcatttttt ctattacttt agataaagct acagaaattt ttataccttc acaacgcgca
    18061 tcttggattt caataatagt atcatcaatt agtttttgaa tttcttgcct atcaacatat
    18121 tcaatcttag aaagagaatc ttttaaagct actaatgatt caacgatttc ttttttctct
    18181 tcagacattt tcttcctcct tctagtgacc cgtaaagcca tgtagagcag agtccattga
    18241 accagtaatt acactatcta caacgccatt ccaatttact ctacattttc catttttcag
    18301 atttgtcggt ccatacggat agcctttttg acattcaatg ttcggattat accagatagg
    18361 attataccat tcagccgcag aagcagattc agtcgataca aacaatccca caccaacaaa
    18421 tccaatacac atcacactat agccaattag ttttttcatc tcgtatccat ctcctaaaaa
    18481 atagaatagt tcttatagtt attcataaga catatttttt ccgtttttgt tttatccttt
    18541 ctgtcctata acaacttttt tcaagtaaag aatgacatag tttttcttaa aaaaacagtc
    18601 tgtttttttc tatttcttcg gtgtataata aatatgagtg ttcatttttt attttaatac
    18661 gcactccgta cataataaat cttgatcgca tcaatctttt gagcttcttg atctactttc
    18721 aattgaagta atacaaattg agtaacctta ttcattgttt cttctccaaa taacaaatat
    18781 tgatccggat tctctatatc ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt
    18841 acatgcgggt ctacatcaat actattgtct ttaatacaac gatctgtcat atagtccctc
    18901 acagcttgat tgcgtttata aacatctgtg aaattactcc attttctagc aaaatctttt
    18961 aagaaatttt ttgtcatttc atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa
    19021 ccaatttgac tgttactttc agtactttga agagttccac ttgtttcttt tactgcttcg
    19081 attgtttctt ctgtcgttct caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa
    19141 ccagtaagac ctatcaaaca taaccaacta aatactaaaa ttatcccttt tttcacgttt
    19201 ctagtcactg cctttcttat ttcaccttac gaaccagact acgaattttt gtttgattgt
    19261 acgtgcgttg ataccgagcc ataatccgtc cttgctcggc attctgttcc acactagaga
    19321 aatgttgtcc gccatatgaa acatctgtta taattccggt gtgaccatag attccaggag
    19381 acaatgcacc acctgcttgc caattcacca catctcctgc ttgtaaatca cttggttttg
    19441 ggttcatgat tacttgccaa ccataggttg tccagttata atcttcccct attaacatcg
    19501 caaacatgtg tccagaaccc attaactgtg ggccacctaa ttgagataca taccatgccg
    19561 taccaccata acattcccca ttatttacgg atcgtccaat gatccgatcc aaaaccttcg
    19621 cattgccttt actttttcct gcaataagcg aagttttttt tgttggtagg ctattccaat
    19681 gcttgatcgc aaaagtatca tattccgtta agttatagga cgttatgata ctcattgctc
    19741 ctcgataata cggactccct tcggtaatac ctgctgccgc atagcctcca cgacctagaa
    19801 tcaataaaca acttccagga tctttattgc ccacacattg attaaagttg ctttggctcg
    19861 ccaccataaa gccataatag tcataacaag cttttaagtt cggaaacttc atatagaaac
    19921 caccttcaga accaccaatt ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc
    19981 aggtaatccc aaaaaagttc gtatcgtttc ttccagattg tgactgtcca taagctgatt
    20041 catagcctaa ttgagaaaga atcacactcg gtaataactg atactttaca gctgcaccaa
    20101 ctgctgcttg cgcatgttca tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg
    20161 gtaatccttc ggtatattcc ccattaaaag cgacagctag acctgtagct tctcctccat
    20221 gctcagaaag ttctcctgaa aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa
    20281 gaagacaacc tagacaccct ttcttcattt cttcctcatc ctttcttcga taatggtttt
    20341 tggactgttc gtccttttgg taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc
    20401 agtgttttag gatccttttc tattttttcc gattgctctg ttggcttatc taaagaagaa
    20461 aatgcaggtt gtaacgattt tggtgcggaa atcgacggtt tactttcaga cgaacgttgc
    20521 cccggttgtt gcacagaacg atttcctgat agagtggcta ccgacgaatt aggaacagag
    20581 tattggttca ataactgctg cattgctgga taactcccat ttttcgcctg atttttcact
    20641 tggcgaatct gttgtgaaag agaaccagca gctgttaatt gtggcacggg tacttttcca
    20701 gaaggcaacg gaatcgattg accaacaaaa gcgatacctt tatttaacaa tggttttctt
    20761 tgattattcc cttgttgctt actcaattgt gccaatgatt gtacctgttg ttttggtctt
    20821 actgaattca tggacgaatt tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt
    20881 ttcgatttgg tagaagacat ttcaggctct accccatgat aacgaacatg acgaccacta
    20941 taaggcaagg cttgatttga acgcccacct agttcaaagg gattaaccac ttgcctccct
    21001 tttaccgatt cttgtggtgt acgtttttgt tgaaactttt gaaatccatg tatcgctgca
    21061 gaagcgagtg gattcgttgc tcgatacaca ttccctgcta atcctgcacc tttgattgcc
    21121 aaattttgtc cagcagttga tagttgacca ggcagttgtt tcatttgaga caaagaatga
    21181 cttgctatgt ttttcgtgtt gtttatagtt tgatctgcag ctcctaaccc tagtttcttt
    21241 aataaatctt tccgttttac aatgagtaaa attgatagga gtgctttgac aaacatattt
    21301 cctaaaaaac caaaaacagt attcattgga attaaatcgt ctactatgtt atagatcaaa
    21361 aatgccacca aaataatcac actgtaaaaa gatttttgaa acatcaagcc caaaaaggac
    21421 tttaaggtat tcatgagcat ataatcaaat actggaacaa aagaagcgag taatgaaaaa
    21481 ggaatcacaa gtaataaaca caacaccaac atttgaaaga taaaacgaat catgccaatc
    21541 actacaagtg gtaatccaat acttagatta agcaatggca ccatagaacc aacgactgct
    21601 ttataaacag ctttagttgg ttgaatatag acacgataag ccttcttttt ccctctatct
    21661 tctcctgcag cagtggtcac ttttgtctca atatcagata attcttcact tgacgcttcc
    21721 ttggctagaa attcttgtgg ttcaatctct gcttgataca attcttctaa atttgttgtc
    21781 ccataattta acaatatata cggatcaaca acagccgctt tataataaag ttggcgcact
    21841 ttttgaatgg cttccttttg tgtcgttggc actaaaacac cattgacatc ttttgtcgcc
    21901 aattcatcta gactatttgt agcagaaaaa ataaagcctt ctacctccac ggacaaatca
    21961 tttaaccctt tggtaaaagc ttctccttta ctaggtacgt tactaccata gccaaaccaa
    22021 accattgaaa aaatgacaac gaagagaaat cgaataaaca ccatttttgc gtaatgtttc
    22081 cctctaaata tacgaagagt aaatagatat aacacataca agacgacaat ccccgcacca
    22141 ataggatcgt atatttttga ataaatagta tccgaaaaag tgaaaaactg gtgaattaac
    22201 tcgttcattg cagctccttc atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc
    22261 aatttactta aactaaagaa catattcgtc atcattttta gaaaatctgt gccatttgcc
    22321 ccaaataaca ttttgcctaa tgaaaattca ctatcagata aataagaaga aaaggtatca
    22381 atatgaaatt tcccaatctc atagatcgat tgatccacct gcttttctgc cgcatacacc
    22441 aatccaccaa caaaaagcag tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc
    22501 ttttccttat gctttatata agccattgtt tcacctcatt tctattgcat gattaacgta
    22561 tcctgttctt ctacttgaga gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt
    22621 tcttcaaaca aactgtgtac attgagtttt cctacacgtc catagggatc taacatccaa
    22681 cactgtcctt taatcatgtg gctcatttca gctaagttcg cttcatttac ttccaaacgt
    22741 aaatgattca aaatcttttc tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa
    22801 ttcccttttt ctttatctga acctaagtca ccaataaatt gtgtatctaa cactaattga
    22861 ttcatctggg aacgccccac acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa
    22921 ttattaaata cccatgcttc tgtaaaataa atcgctgttt tcttactact atctcgtttt
    22981 ccaaacattt cacaaaaacg acctaaacta atcatcaaac acaaagactt tcgatccgca
    23041 tctgtatata gttctggtcg gacattgtca tttggtaatt tcaaatcttg gatctctaaa
    23101 atcgttgttt tggcattaaa gtctaatccc gggttttctc cataagaaaa tcctaaacgt
    23161 aaaatcgaat cttctaccgt caagcgtaaa aactcaccat attctcgaac ctctttttgt
    23221 tccatttgtt ctaaatagtc gaccacatgc atcaagccaa cttgttgtcc ggcttctttt
    23281 tgaactaaaa cttcttggat tccctttaat accgcccctt ttaaaaggtg attggtacct
    23341 aacggactta actcatcaat catatcctgg gctacttgtt tcgcatctac tcgggataaa
    23401 caaacaattg gatctaatac accataatta cggggatttg tagcatctaa cgtgacataa
    23461 ctcatttttt gcaaatgttc tacaaacaaa gggtaatgtt tctgataata cgggtcttga
    23521 atgagtttcg taaaccattt gcgcttttct cctttgggat ccacaaataa actttgtaca
    23581 ttcataaacg aactatacaa aaagagcaag ccaactaaaa aggtcttccc tttaccggtt
    23641 tttccagtta ctgctacatg aggcgcatca aacaacgcac ctttgattcc ttccgcaatg
    23701 gctagaagat tcaaaaagac aaaacgattt gacgcataaa ttaattcttc taatggtgtt
    23761 ttttctcctc gagaaccacc atcccctaat gtatcttgcc gaccaatata ccagccggat
    23821 tccataccta attctgatac tgctgaaaac aaccattcgc caactgcttc tacagttgtt
    23881 cggtgcttcc aatgtaactg ccgtcccaat ggttgcccta ataaacattt atagaacaat
    23941 tctcgttgat cattttttgc acggtggata tggataatcg tatgaagttg ttgaataacc
    24001 gttgcactat tttgacgaac ttcttcaacc gtttgaccgt atacaataaa acatgcgatc
    24061 caatcgacga caggttgccc attatctact cgttgcttta aatgttttac taatcctcga
    24121 acagttaata agcgttcact aggttcttca tcattttgaa ccaattcctt atgctctgtt
    24181 ttgaaccgtt ccgatagcca attcagtttt aaaccaatac ctccctgtcc tttcaagcgt
    24241 ggatagtgaa ttttcattcg caattctgct gggaaagaaa gatcctgagc aaattgaaat
    24301 agatcacaat ttctcatatt ttcaggtgtt tccccaatag gcaacaatgc taaataacta
    24361 ctccctaact catcttctaa ttgaagaagg cctttatgtt ccttcggtat acgtacatac
    24421 gttccttgat tgtctttcag tacttcccaa ttattttgtt cttctacttt gtgagccatt
    24481 cctcgaatat attgcagacg attgagatac aacagctctt tttttactaa gggacgaata
    24541 gaaaagctat aaagtaattg tgtcacttct tttacataag gatcactttc agaaaacaaa
    24601 gtttccaaat catctacttc taatccaaat aagcgtgcta aacttcctgc taaatctaac
    24661 cagattagat ttattacatc atgaaaactc tccaccgttg ctttttcttt gactttgatt
    24721 cctaaaataa atgaaggacg atagacttga ccttctcgtt ccaacaatgt cactgtttct
    24781 tttgcataat acgcaccaat tgcttgacta tcgggagaaa atccctttcc taatgtgtca
    24841 aacttcgtcc gtaaattcat tgttcgtgga tacatcacca actccacatc ttcataatct
    24901 gctaaaagca acagtaaacg agccatcatt tcttttattt cctgtgtctt ttttggtgtg
    24961 ttttttgaaa ttgttcgtgg aaaaacatga tagtaggccc agatacaatc ctctccattt
    25021 aacaaacaat ttttcttgaa tgcttttact ggttctttta caagcaccaa tgaatttcac
    25081 ctcccaataa aaaaggaaca accagttgat tgtccctttt tctaatcctc taaataaaca
    25141 tacttattca caaaaagtaa ttcttccaat gcattgtttg ataccttgtc ccagttataa
    25201 tacacacaat caccattcat catacgaatc atataacaaa accaatccca taaataaaaa
    25261 tgcacttttt ttccatcagg ttttagtttt gccagtgcta acacacataa ataggggagt
    25321 cctatataaa aaacaactcc tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa
    25381 cgtaaaaata aatattcaaa gattacgacc aacaaaaaag ttgctgcatt gactaatgca
    25441 actggaaatg gaagacgcca attgcttgta atctgttgta cgcgataagg ttccgcataa
    25501 acaatcgaat aatcataagg ttcccaatcc acttcactat cctcctaatc cataaataat
    25561 ttttcaatga ttttaccaaa cgcatcaaat acacgtgttg gatcatttaa gaagaaataa
    25621 actgcggcac ctgctgcgat agatattaat gcatttccta cttttgattt cataaaattt
    25681 cgcaatccca gataaacaac caccattatt aaaaaatagc caccttgcgt taatataaaa
    25741 tctacaattc ccttcagtgt catttccgtt cttctccttt cctatccaac caattatgtg
    25801 ttaatttttc aacataatac tgatttgttt ttttcgtaat gacaatcgtc atttgttctt
    25861 tttgtaaaag tgacgtatca ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt
    25921 ttttcgtttc tttataacgc aatactttca tctctacttg atcaacttta gctgtttgat
    25981 tgagtccttc aggttctttc atcatgtatg acatatcttt tgcattgttt ttcgcatact
    26041 tatctaagaa atcttccaca aatccttcaa catctttcgc ttccgacgca tcaaccgttt
    26101 cgtataatgt gtcatcataa gtaatcattc gttgtttttc tgtattcttt ggtagtgccg
    26161 taaaataagg ataagcaata atacgacagc ccttgtttga aatagaaaag ggaagattga
    26221 gtacttgtgt gattgtcaca cttgtttctt tcttactctc tggattcatc gagcgaatat
    26281 cataagttac tttataacta gcagttggaa tggtttcaat ttcttttaat ccaataaacg
    26341 aagcagcagt taattttcga acacctgaaa cacttcctgg ttcttgaata aagttttgtg
    26401 cataaaactc tttttttaat gtttcttgac gttgttgttc ttgttcatga ttatcgaatg
    26461 tattcatata caaagcaaca aaacgattca taaaggattc aatttcaggt gtgtacgtta
    26521 attctgaccg actttgtact tctttctttt gtaattgttg gatttcatct ttcaaaaacg
    26581 acacttgaac cttcgttttc atggattgaa aaaatgccaa gccccctgaa aaacaaagaa
    26641 ataaaaccaa acaccaaatc catttcactg ctttttttga atggattttc ttttgcttcg
    26701 gtactttctc tgtataaact ttttctttcc gccgtagttg tcgttttttc ataagctact
    26761 ccttctagta tgtttttact agtacgtacg attgacaacg ataagcttgt ttttctgaaa
    26821 aatagtccat tcaattaccc tcattttcta ttccttttct ctttctttta tatccaacaa
    26881 atccagcaat acttgtcaac actcctccta gaacagaaag tgcaacatgt atttcttcac
    26941 cagtctttgg taagactttt gtttttccat ctgctgtttt gatttcaata ttgccacttg
    27001 gaagtttctt tacaggaatc gacaaatcct ttaccaaatt cccttcacta tcttggatta
    27061 atggaatacc atcttttaca ccaacaattg tttgatcacc tgctactttt aatggggtgg
    27121 ttggtgttgg aatatgggtc aattcctcta cattactagt gacaatcggt acactcattc
    27181 cttgagaagt attcgtcgta atttcaccgc ttggggttac cgatactgtg acttcttttg
    27241 ctggaacaat aggtttattt tcttttggaa ttgcttctga tttttcaaca ggttgtttcg
    27301 gttcttcttt ttgttcttca ctggtagacg acatcgttgg ctgttccgta cttgacgaat
    27361 cagtattctt ttcagaagaa ctggttgttc ctgattgttc cggagttgtg actatacttg
    27421 aatccgttgt tgttgaacta tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg
    27481 attcaggaaa ctctggttct ggtgtcactg gctctttttc aggtttctct gactgcaatt
    27541 gttcaggttc tgctggtttt tccgttgttc ctccatttag ctctgtctca acatctggta
    27601 atccttcccc tggcaatgtt tctacaattt ctgacttagg tgtttctaca gtttcatctg
    27661 cataaaccaa tgaactccct aattgaaacg aaaataatga aattcctagt gttgttacga
    27721 ctgctaatgt tgtttttttc atgattattc ctccaatttt ttatgcaatt cgtgttttgt
    27781 tcatagacgc cgcactaata ttatctgaac gataaaagaa ctcccgtccg ttttcttcta
    27841 acccaacata tggattattt ttgaataatt gaatcaatag ttcactggtg ttgtattttg
    27901 gatagtttgc aaaaaaataa tactgtgctt gtcgcccatc cgaaaattca acaagtaatt
    27961 cataacgata ccgcttcaat ccttttcgtt gttttaaagg atctctatac caacttgtcc
    28021 atcgttccat tcgtggatag agatagacac gatacacata gaacaatgcc ataccacata
    28081 ccaccaaaaa taataatcgc cacataaaaa ttcccccttt tcttctacct ttcttgaatc
    28141 atcgaacatt cataccctct atagagattt gaaaaatcca tcatcaattg ttcatataaa
    28201 cgttgttgca aatagcgatt gtttcgaata cataattctt catagccatc aatcacttga
    28261 tcttcttttt catagcatct tgtagctcta tgacgctcag cttgcaagac ataaccaaac
    28321 tctgcaccat atcgttctac tgttaataac tgttgactac gcaataatgc aatataacaa
    28381 tcacaaaaat gttcaaccga catttgattt ttctcacgtt tgtgaaatag gtattcttta
    28441 gaaagcacat tattcgttaa acaatctgat aaatagcgat aatctggcgg tcggttattt
    28501 tcatctgtga atttttccca taataaactg atttttattc gttcagcggc tagatagtct
    28561 tgttcgcgtg gataaaattc atcaatcgca caaagttttt tatcacctga ataaacaaat
    28621 ccatataatg cgaaacgaac tcttcctttt caattggctc tcctaaaaat tccagtgaca
    28681 taccatcttc tccttcctaa aaaaaataaa aaagcagaaa ccgatttggc ttctactttt
    28741 ctttcctttt atagctcttt tttacggaag aatccgcaaa tgacattttt tcaaaaaatc
    28801 aatcggcaca agttcacgaa aacttggagc aattttggat agaatcactt ccccatcttg
    28861 taaaatacaa tcataaaaat catcagttac ttgatactta cgcttcttac aataattgat
    28921 aaaatagtag cgatcatttt ctatttccat tcgttgttct actaagcgca tacgaacatc
    28981 atcatagaaa taagccacaa atcgcccttc aagttcatgt ttttcaatgg gatactcttc
    29041 tggtaacaat ctattttttc gtcgtttata cattgctgga taccttagaa gaagttcatc
    29101 taaacgtctg tcagtatatg gtaactgacg aatcaacgat tctaatcgtt ctttatacag
    29161 atagaataat tggcgcaagc aatagtgtac cgcctctgga ttttctaata ctgttggatc
    29221 acgtgaccaa tacgcaccaa cataaatcat aatttttttc atttagacaa tcacaccagc
    29281 aatactaatg accgatccac aataggtaga acaagtggca tagcgaccaa tttcctctaa
    29341 ttctatctca atctcttcta aagaaagata tgtctgttct tctgttggct ggcgactcat
    29401 ttcccaagtg acagtcacca atgaaatttc ttccataaga ggtttttcgc tgctaagtgc
    29461 tgccacacag ttttcatata tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc
    29521 aggtattggc aatgaaaatc gataaaagcg aatgaattct agccttcctt tccttcggct
    29581 aaattccaac tcttcgagtt cttgttcgta tcccttacga acttctaata agcgtgcaat
    29641 ttcttgccag ttctcttttt cctgataacg tccagctttt accgcttgtt caatataagg
    29701 aactcgaaaa cgaaaataag acaaatcaac catcttttca tttaatgctt cgattgcagc
    29761 ctttaaatcc aacaggcgca gccattctgt tgtcgtatat gcattcatgc aattttccct
    29821 cctgacgttc ccatccattc tagatagttt tgttgctcgt catttagttc ggcattatca
    29881 atcccttctt gtacaaagtc tcttcctaaa atacgatctg cttcttgtaa caactttaat
    29941 gtcggagcta cttgtttcaa aaaccagcgt tctgaacgat ctaatgagaa tttttctggc
    30001 tttactgtaa aatccaattc ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca
    30061 atccgtgcat cactttccag tctatcccaa acaataagtt ttgcattaat taaaccaacg
    30121 ccaatctctc ctaaacgttt cggtgaatca cctaaaataa atgcttctac tgcttgtgtt
    30181 gctttctcat tggctaaccg taactcatat cgattataaa taccatattt cgttaatgct
    30241 tctaattctg aaattttttc tcggttcgca atttcaaaac gtttctcata aaaattaaaa
    30301 aagagaggac tctttcgcga tccaaaatac aaagataatc cttcacttcg aaaatagcca
    30361 tcatctaaaa aacaaccgcc aatattctgg aatctcttaa agtccatttc taataaacct
    30421 gctttttgtt tttctagtaa ttcaaagaga tcgaaatgtt cttccgtttc caatccccaa
    30481 agttcatcta acgcaatatc gaatcggcta cattgcacac gtgtataacc agcttccaac
    30541 tttccttgat ttttccaaat ggaatatagc catgttcgcc aatctgtttg ttgttcacgc
    30601 atgacttcct catattcccg gcaaccttgt cctgaaaatt gcacaagtac attttctgtt
    30661 acagttcgtt ttgaatgcca gaacacccag atattcccac gaacagaaag acagtcaaaa
    30721 agatacaagc ccgttggacg tgtttcaaat tctgtcagat ccattcgtaa tacacgttga
    30781 atcactgttt tgtagtctgt cccaaaaaag gaaagacgca aataatcaat cattgctcga
    30841 agatctattc cgcttttcat atacaatcga aagcgttcct tgattcgttg ttcaaaatcc
    30901 tctgggattt ttcttttccc ttgttccatc tttctaatgt aatcttttga gcagcccatc
    30961 aattgcgcaa atcttgcttg tgatagcggc tttttatctc cacttatatt atgaattgca
    31021 cttaacttct ttctgtagct ttttagctca atcggtgtca ttttctcgct ccttttataa
    31081 aataacaaaa tcgatttttt ttaacccttt agaaacaccg atatatcaac tagtccccct
    31141 cgttttttgg gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt
    31201 ttataaaact tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg
    31261 ctttacacaa agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta
    31321 gcgagtgcgc attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc
    31381 ggctaccaga ctacgtctag agccctcccg atagataata actacaaaat taaatgatct
    31441 aattttgtta tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa
    31501 tcctcttcta aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc
    31561 gttagcaata cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg
    31621 taatcagtta cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa
    31681 ggattaatca ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt
    31741 cacctcctta gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt
    31801 tgctaaatac atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt
    31861 ttgatcttga ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc
    31921 aaattctttg ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac
    31981 atcaaatcct ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt
    32041 tttaaaatct tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat
    32101 acggaatgtt aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat
    32161 tgctaaaata atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt
    32221 atcgactcta tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa
    32281 aaagataaaa tgcggtggta acccataata agcataattt ttccctggtt catatttagg
    32341 atctttcatg gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa
    32401 acaaataatc atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc
    32461 acctaatgct gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa
    32521 acaagcttga attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat
    32581 caacatattt ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc
    32641 attttctgct atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt
    32701 gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa
    32761 atgacccatt tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac
    32821 taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg
    32881 tttctcacta acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag
    32941 taactgacgc caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa
    33001 ccctaaaatt aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag
    33061 atacatttgc caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca
    33121 acctaacaga atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg
    33181 attgaaatag cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat
    33241 tcctcctttc tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac
    33301 aggaatatca catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg
    33361 gtgtgcacac atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg
    33421 tttccactgg tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat
    33481 aaccgcacaa atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc
    33541 aatcaattgt tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta
    33601 tctgaattca aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc
    33661 ctattctttc aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt
    33721 tttgatctaa acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta
    33781 ctttctctaa tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg
    33841 ctggaatata taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa
    33901 aaccagtcaa atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg
    33961 gatttccaaa aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt
    34021 cattatcaaa tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag
    34081 tttcatctaa tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa
    34141 acctccccaa tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga
    34201 cgctctgttt gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca
    34261 aaataagctt cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc
    34321 tcttcagctg tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt
    34381 aattgaacat atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa
    34441 cccaataaaa atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata
    34501 aagtttttcc tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa
    34561 caagcggtta aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt
    34621 tataaagtgt tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac
    34681 tacttgtttg ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt
    34741 gttctggttt cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt
    34801 tgtttgttag atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg
    34861 ttttcgcttg gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca
    34921 ctaagaattc aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat
    34981 gagtgccttc aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga
    35041 tttgaccaat cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg
    35101 ccaagatctc ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga
    35161 ttttgatggt ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc
    35221 taaacagatc ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa
    35281 agtctagatt gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt
    35341 cttttccatc tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg
    35401 tgtatgtttt tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg
    35461 ttaatggatt aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg
    35521 ggttagttac tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt
    35581 catcatttct aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat
    35641 caaaggtaaa tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg
    35701 tttctaccgt atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc
    35761 ctttgaccaa gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg
    35821 taaacaatcc tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa
    35881 cggttggatt attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa
    35941 cttcaatgcc atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac
    36001 tagcattaac cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca
    36061 aggtacttgt gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca
    36121 atactcctga aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt
    36181 taatgacgat cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt
    36241 gtgtttctag ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat
    36301 catctggcgc attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga
    36361 aactttctaa tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta
    36421 aaatttcccc gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca
    36481 aggtaaagtt cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata
    36541 attttacact tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat
    36601 cattggtaat cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga
    36661 tttcttccaa acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc
    36721 ctttttcatt ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga
    36781 cataacgatt ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt
    36841 ttcctgtttc ttgatccact ttcaccactt taagacgttg ttcaattgct ttatcttcta
    36901 aaacataatg gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt
    36961 tatacccttc ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt
    37021 taccaaaagt agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg
    37081 tttttgacgt caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg
    37141 aatcgactaa atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat
    37201 cttttgcggt cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag
    37261 aaagcacata accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat
    37321 ctttaatttc tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt
    37381 tcccatctgc ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt
    37441 tcacaccagt ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta
    37501 aattaataac agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg
    37561 gacgacgata ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt
    37621 tatccggtgc ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga
    37681 caccttttgg tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt
    37741 tcaacgtttc gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa
    37801 agttattaat caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt
    37861 tgattgggat ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt
    37921 catcggattt gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc
    37981 gtacatttga atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg
    38041 cggctgagac accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca
    38101 tataatttaa agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc
    38161 aatcaatttt ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac
    38221 gattggttgg tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt
    38281 ttttcttttg attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt
    38341 gactagctgc ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt
    38401 cagcagtagc cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt
    38461 tctcagaaga ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag
    38521 tggacgtttc tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac
    38581 caggactatt ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat
    38641 tgaccgttgg cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc
    38701 cggcttctgc tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg
    38761 taggttgtat agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga
    38821 attgagaaaa agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca
    38881 caagaattcc tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag
    38941 tgataacgac gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc
    39001 atcaatgatt ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa
    39061 atcaactgtc catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa
    39121 tctcatgcca taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc
    39181 ttcatctaat gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag
    39241 atcttctgga agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc
    39301 attactagct actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat
    39361 tcttctttta cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta
    39421 aaaccatcta ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa
    39481 taggtaggtt tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca
    39541 aaataactat ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt
    39601 attgatactt gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat
    39661 aaatattccc gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc
    39721 cccattcgta taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc
    39781 gtcccaacca atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa
    39841 tgccataagt tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac
    39901 acgtgtgcct tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg
    39961 cattttttgc tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct
    40021 tctttataaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40081 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40141 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40201 tttactagaa cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa
    40261 aaacgataga tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc
    40321 attgcttatc aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga
    40381 ccgtacgaat ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa
    40441 taaccctatc ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact
    40501 atgaccatta ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga
    40561 tctccgaatc tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat
    40621 gtggctctgt acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc
    40681 aagcgaactt tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca
    40741 ttttatctag ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc
    40801 cattgagctt gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa
    40861 ccctttttgt ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa
    40921 aacgtcgata acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat
    40981 tctaaagaaa gatttcctat ataagcatat tggataattc gattagtatt gggtatatct
    41041 ctaccattct catacctatc tatggaccat tttgaaattg aaggagaaaa aagctttcca
    41101 aactcctccc tttctagata ataatctttc ctaattttcc tcatccttaa tcccatacta
    41161 aaagaattta tctttttaaa atcgctaact ctttcatctt ttggaagtat attcttataa
    41221 tcttctccgt acataaggta aggaacagtg accttaccaa gataggcaat attcattagt
    41281 ttttcaatat ctggaatatt aatacctttt tcccatctac ttataacatt tttgcctgta
    41341 tagatggtaa agtttttacc aaatttttca agcgtctctt cccggttctt tcggacttct
    41401 cgtattcttt ttcccataac aattttatcg tcttttgata aactaaatat ctcactatca
    41461 cgattaatct ccttcaaaat ttctttttca ttacttaata gaaaaatatc gatttttttt
    41521 tgcatttctt ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta
    41581 attcctatat tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt
    41641 agtttaccga tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc
    41701 agaatcttgc ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat
    41761 ttaactaaag tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt
    41821 ttctcttttc ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca
    41881 cctcctatga gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc
    41941 aattttgtta attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca
    42001 gataatcctc agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt
    42061 ataagttatt tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc
    42121 ggaacttcga cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag
    42181 agtttacgtt tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag
    42241 gctttgatgt ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca
    42301 aattgttggc gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag
    42361 gcaaagtctc ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg
    42421 tccatcttca ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag
    42481 cctttcgtgc ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata
    42541 ttatagacga catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt
    42601 tttcgttgag aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct
    42661 ttgacgaatg gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta
    42721 tctactgtag cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc
    42781 gtgatcaatt ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga
    42841 agtccgagga atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat
    42901 tgtaaagcag catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga
    42961 gaagtatcaa ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt
    43021 aaagcttcag cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg
    43081 acagttttga tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca
    43141 actgcttttg gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc
    43201 agtttgattt tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca
    43261 ccgcggtaac tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct
    43321 ccacttggtg caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct
    43381 gatttggcac tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct
    43441 tgacggacac gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc
    43501 tccaaatcat tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt
    43561 gctgcatcga ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag
    43621 gtgaagtcga cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc
    43681 gctgtgtaat tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa
    43741 tccatcgagt ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct
    43801 aaaatacgat cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta
    43861 cgaatctgct cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata
    43921 taatcagcac tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc
    43981 agctatagaa gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga
    44041 tgaaaaaatg gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga
    44101 acatatcgat caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat
    44161 cgaggaaagc gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga
    44221 agtatcgaaa gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta
    44281 tccttgtgaa gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg
    44341 aacttatcca gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga
    44401 taatatgcgc aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta
    44461 tcggcgtaaa ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca
    44521 gctacctaaa caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga
    44581 cgtattttcc gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga
    44641 ttttcattgg gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta
    44701 ctacgatagc aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt
    44761 tcacgtaaaa gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat
    44821 ttccgcacat ttttccatga tctactgggt acatggagat atttataaaa atgagccttt
    44881 agcatttttg attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg
    44941 gatcgttgat gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt
    45001 tcatttcatc attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg
    45061 ggatgaacag ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga
    45121 tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat
    45181 cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat
    45241 ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt
    45301 ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa
    45361 tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg
    45421 acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg
    45481 ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg
    45541 aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac
    45601 agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat
    45661 ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt
    45721 cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt
    45781 aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat
    45841 aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg
    45901 gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa
    45961 cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt
    46021 ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg
    46081 gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat
    46141 gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga
    46201 ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat
    46261 cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat
    46321 acgatgggcg acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg
    46381 tctcaaagta aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat
    46441 acctaataaa agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc
    46501 gaagaaacta cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt
    46561 cagacccctt ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc
    46621 tgttttgaaa tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac
    46681 atgtcctgca acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt
    46741 caatggtctt gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc
    46801 atcactgatt gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat
    46861 tttgtcaacg attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc
    46921 gattcggttc aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc
    46981 cactgcttct ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat
    47041 attgctggtc atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt
    47101 caagcgtccg tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc
    47161 gatttcatcc aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc
    47221 accttcttcg taacctacat agcctggagg agctccgact agacgagaca cagcatgttt
    47281 ttccatatat tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc
    47341 agctaacgct ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga
    47401 acctagagga cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac
    47461 cgcatcaact gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat
    47521 tagtttttct cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac
    47581 gacttgagca atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt
    47641 tgctttagct tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa
    47701 tacagcagcg cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt
    47761 atcgatttca gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg
    47821 catcttcatc gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa
    47881 gcgtttttta cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg
    47941 catcaaacgt ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat
    48001 cgttgcactg gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat
    48061 ataacggtca gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg
    48121 gtggatctca aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg
    48181 ttctttgact aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg
    48241 acggtactca tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg
    48301 tttcaacaga tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg
    48361 gatttcatcg atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc
    48421 ttttagtctt tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc
    48481 taatgaaaaa atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg
    48541 agcaagccct tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt
    48601 attttttgtt tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat
    48661 gattggatcc atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa
    48721 tgctttgtat tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc
    48781 gatgttttta cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg
    48841 ataatttttt aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc
    48901 gaagctttca cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt
    48961 ttgtccatat tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat
    49021 ttcatgttca aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg
    49081 attagattgt aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt
    49141 gaccgcaatc tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat
    49201 attcatacag gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca
    49261 aaaactatta tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt
    49321 ttttctcgac ttatataaat cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga
    49381 gccaaacaaa cgtaaaatta tcttatccat aacgtttttc tttgatttga taaaaagggt
    49441 agatcttata aaagacctac ccttgatttt ctttattctc tttttttact tctattattt
    49501 cttctttgat cactcttcgc cgttcagcaa tcacatacac aaatccatag agaaatggca
    49561 agataaagga actgaaacga tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa
    49621 ctggtctgaa taataacaaa tagacaaatt caaaggaacc aataccagca ggcgtaggaa
    49681 taacccctga aagcactact gcaaagctga taaacgaaat ggtcagtaga aaatctattt
    49741 taggatggtt ttcaatcaga atcaaataag gaagtacata ccaaaataca agttttaaca
    49801 tattccatag ataaatccgc aatagcgcag tcctgtcttg gataatggtt tggaccgttt
    49861 cccttaaaga gtagatctgc tgattgcatg tatccaccca tttccgcaaa cgctcacttt
    49921 taaataactt attcgataag ctgacaaaaa atatttgaac attcaagctg acggacatcg
    49981 ccaataaaaa ggcaacaatc acgatcgtca aaaggatacc agcgatgata aaccagatca
    50041 ttgatggacc attagaataa aaaagagaga attggatgac caagccagct aatgcataag
    50101 tgatcactgc cagtttgtac ataatcatat ggagtgctgt cacccctgct ccctgggata
    50161 gcttcattcc ttttttgtta taaaaataaa tctccgagat cagcgttcct gtaccaaacg
    50221 aaacgattcg gtaaaacgca atatagctag aggtcaagaa cccatctttc gttgtaaaat
    50281 tcggcgcgaa aggttcagca atttctttga ttgagcgccc ttcagcgatt tggtaaactg
    50341 tccctaatat aacagccgct gccaaaacaa caacactggt agacagcaat tcggcaaaga
    50401 tatctgcaaa tgattgattg attaaataat aaataatggc aaaaatcaca atcagcaaaa
    50461 gcagattcag aatgatttta gttttcgttc ctttagacga catctcctgc tccttaatgg
    50521 aaaaaataaa tgagaatcaa atcaacaagt acacctgcag cgaatcctat agtaaacttc
    50581 tttttccgag ttttatgatg gaaaactttt cgagagatca atcctcccaa accaccgcct
    50641 aacaggctga caaatatcaa attattctca ggaacacgcc agccgctagt tctagcctga
    50701 tgtttatcat accccatcag acaaaataag taaaaattca caatgaaaag gtaaaaccac
    50761 aaaggattgt gtaacaatac atccattact cttcttccaa ctttctgtta actactattt
    50821 ctaagctttt tcttctttct ttggtgaata aactgttaat ccttttgata caacagataa
    50881 ttcaactgga agatcatctg tcgtgtctcc atccgttcga gtgcccacag atttttcctg
    50941 cgcttggatt ttcatgcgtt tggctgtgaa ataagtaatg ccggggatcg tatagaattt
    51001 gcctctcatg attctaggca aataaaaaat aaatcggaaa atatctaagg aaggtacgat
    51061 cgttacatga aattttccat catctagggt cgctgagtcg tcaaaatttg taaaaccgcc
    51121 cacggagtta gacatcgtga ctgtcaacag ttgggatttt cctttccaat gctcctcgtc
    51181 tgtttctaaa tccaaatgat attttttctt cttcagcaat aacttaaaaa acttcttgat
    51241 aaagatccac gagccgtaac gctgtttttc ttcttgactg atccaaactg ctgtatctgc
    51301 taagattccg attgtcagtg tagagataat gacttcatca ttgacttgac catagtctat
    51361 tttccgtaca ttgccttcca ggatcacgtc agcagcttct tcttttttga ggggaatctc
    51421 aagaacacgt gcaaggttgt tgacagttcc acctggaagt aatccgacat ggtattgaga
    51481 taatgttttt tggaacgctt ggacgatatg atgaatcgtg ccgtctccac ctataattac
    51541 taaagtatca gcttctatgg cttctgcttt cttgactaat tccgcatcat caatcgatgg
    51601 gccagtttca gctagttcta catataattc tggctgcttt tcttttacat aatcttgaaa
    51661 ccagcgtgct aatttctcac cctcatcttt ccctgaactt ttgttataaa aaactagcag
    51721 cttttccatt ctttacctcc tatttactct tcaaaaaaga gggcgggaca accaattcca
    51781 ttgtcctgcc ctttagcctt agacagatga cattccatca ttgggacgaa cgccttgtca
    51841 taagctgttc tttttcgatt ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg
    51901 tctgttgctt tttccattac ttgtaaagaa acaaattcga atcgtccgtg catattttct
    51961 cctccggcaa atagatttgg cgtcggaata cccatgtaag agatttttga accatctgtt
    52021 cctccacgaa ctggttcgat cactggagag ataccaagct cgatcattgc ttgtttcgct
    52081 aattccacga tgctcatatc tttttcaatg atttctttca tgttgtaata ttgatcatac
    52141 atatctactt tgattcgttc ttgatcaaat cgctgattca actcttcttg gatatgtgtg
    52201 atttgtgctt tgcgtgcttc gaatttttca cgatcatgat cgcgaatgat ataactcata
    52261 gaagcttctt caggattccc atccaatgcc atcaaatgga agaaaccttc gtatccttcc
    52321 gttttttctg gaacttcatc ggctggaagc tgattatgaa aatcgattgc taactgtaga
    52381 gcattgatca ttgtatcttt tgccgtccct ggatgtacat ttttcccttg gatcgtaata
    52441 tttgcttgag cagcactaaa cgtttcatac tgcaactcac ctaaaggacc accatcgact
    52501 gtataagcaa aatctacatc gaattgctca acatcgaatt tatccgctcc gacaccgatt
    52561 tcttcatcag gaccaaaagc cactttgatg gtaccgtgag ggatagaagg attattgatc
    52621 aagatctcca ttgcagtcat gatttccgca atccctgatt tgtcatctgc tcccagtaat
    52681 gtactgccat ctgttgtgat caatgtgtgg ccttcataat ttttcaaatt tggaaaatct
    52741 tttgtattca atgtatagcg ttcttccgca tctagtacga tcgtagactc gccatcatag
    52801 ttttcaatga tttgtggatt gactcctact gcattaaaat ctgctgtatc catgtgggaa
    52861 ataaatccaa tggaacgcac ttcatgatca acattgcttg gtaatgttcc cataacgaat
    52921 ccgttatctt cattatagaa aacatcagct aagcctaact cttctaattc ttttttcaat
    52981 tcttgcgcaa aagcaacttg tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag
    53041 cgtgtttctg ttttgacata tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc
    53101 tctcctttta attaaattga aatgggtttg tatttgtttc agaaacgaaa aagttaatat
    53161 cccaattttc ttcttgtttc caagattcaa atttctcgat aaatcgttgc ttgcataacg
    53221 attcaatata atgaccggga tcaattgcaa tcaacccagc gctttgcata tcatgagccg
    53281 tatgataata gatatctccc gtgatataga catctgctct ttgggcgatt gcttgcggat
    53341 aaaatttttc accgcttcca ccgcaaatcg caatgcgctt cacagaggat tttgcatttt
    53401 ttggctggac tattctcagt ccatctagtt ggaacgcttc ttttacttgt tctacaaatg
    53461 cctctataga aatttcttgc ggcaattcac ctactcgtcc taacccaaac atttcgactg
    53521 gttcatctat agcgaaaaga tcataagcag gttcctcata aggatgagct gaccgcatgg
    53581 cttgaatcac ttgtttttcg atagtttccg gcaaaataac ctcaactttt gcctcttgaa
    53641 cttgttctgt cttccctact ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc
    53701 gaccgtgtcc aatcgaggta aaacttgtac cggtataatc accttgtgta ccagcaccag
    53761 ctgcagctaa tacttcacgc attttctgtg catgatccac aggtacataa acggccagtt
    53821 ttttataatg aatctcatga gtctttacta aataactctc cacttcaata ccgagaagtt
    53881 cacaaaacca gtcattcagt ccatcccaaa taatatccat gttcgtgtgt gctgcataga
    53941 cggcaatatc atgtttcaac aaatctgcat acattttttc ttgtggctga tcagtaacca
    54001 gccgttttac aggacgaaag attggaggat gtttagcaat cagcaaatcg atttttttct
    54061 tgattgcttc ttcgaccact tctggacgaa cgtctaatgt catcattact cgttggattg
    54121 gcttatccaa tgttccgata tgcagtccga ctgggtctcc ttcttcagct agccactgag
    54181 gacaatagct ttcaaacttt tggatgaaag tgcgtccgct gattgtcatg gctttaaaac
    54241 ctcctcaatc atttggattt cctgctcgat ttgtttttga cgtgcctctt gattagctgc
    54301 ttgtttgagc tgttcacgga tagcttttct ttgttgaagc tctcttgtcc attttgattt
    54361 gaagatctca cttttctctt caagcagaaa tggcccgaac atcagctcca tttcattgta
    54421 agtaactggt ttttgttctt tttcggctac aatgatttca tatatttttt tattttcttc
    54481 aagtattttt tctgattgga ttttatactg attttcttgc agccaagatc gtaatgtacg
    54541 ctctccgatg tttggctgaa ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt
    54601 gccagcttct aggatatctc ggatcagtga acctcccata cccgcaatcg tgacagctgt
    54661 aataccatct tgtggctgga tagcatctag accatttgca aggcgtacgg tgatacggtc
    54721 ttctaatcca tcttttttga cttgcttacg tgctgattca taaggacctt gaacgacttc
    54781 accagcaact gcatattcga tttttcctct taatatcaat gcaacgggta agtaagcgtg
    54841 atctgatcca atgtctgcta aacgagcgga ttcagggaca aagtcaccca ctgcggctaa
    54901 gcgctgagat aaatctaaat ggttcatagc ttcactcctt ctagataatt atagcatttt
    54961 ccaaataaaa taaggagact aggaataaaa aa
//
LOCUS       DACIFS010000012        52449 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.012, whole genome shotgun sequence.
ACCESSION   DACIFS010000012 DACIFS010000000
VERSION     DACIFS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.012"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            1..794
                     /locus_tag="IYA83_000711"
                     /pseudo
     CDS             1..794
                     /locus_tag="IYA83_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            934..1860
                     /gene="cysK"
                     /locus_tag="IYA83_000712"
     CDS             934..1860
                     /gene="cysK"
                     /locus_tag="IYA83_000712"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ8586165.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2096..2542
                     /locus_tag="IYA83_000713"
     CDS             2096..2542
                     /locus_tag="IYA83_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ8586166.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2814..4166
                     /locus_tag="IYA83_000714"
     CDS             2814..4166
                     /locus_tag="IYA83_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ8586167.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4337..4624
                     /locus_tag="IYA83_000715"
     CDS             4337..4624
                     /locus_tag="IYA83_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586168.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4850..5293
                     /locus_tag="IYA83_000716"
     CDS             4850..5293
                     /locus_tag="IYA83_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8586169.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5299..6483)
                     /locus_tag="IYA83_000717"
     CDS             complement(5299..6483)
                     /locus_tag="IYA83_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ8586170.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6751..7449
                     /locus_tag="IYA83_000718"
     CDS             6751..7449
                     /locus_tag="IYA83_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ8586171.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7456..8133
                     /gene="nth"
                     /locus_tag="IYA83_000719"
     CDS             7456..8133
                     /gene="nth"
                     /locus_tag="IYA83_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ8586172.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8259..8792)
                     /locus_tag="IYA83_000720"
     CDS             complement(8259..8792)
                     /locus_tag="IYA83_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586173.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9089..11470)
                     /locus_tag="IYA83_000721"
     CDS             complement(9089..11470)
                     /locus_tag="IYA83_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ8586174.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IYA83_000722"
     CDS             complement(11460..12107)
                     /gene="recU"
                     /locus_tag="IYA83_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ8586175.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12171..12710
                     /locus_tag="IYA83_000723"
     CDS             12171..12710
                     /locus_tag="IYA83_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ8586176.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12800..13219
                     /gene="gpsB"
                     /locus_tag="IYA83_000724"
     CDS             12800..13219
                     /gene="gpsB"
                     /locus_tag="IYA83_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ8586177.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13241..13608
                     /gene="rnpB"
                     /locus_tag="IYA83_000725"
     ncRNA           13241..13608
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IYA83_000725"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13817..14983
                     /locus_tag="IYA83_000726"
     CDS             13817..14983
                     /locus_tag="IYA83_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ8586178.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15029..16525
                     /locus_tag="IYA83_000727"
     CDS             15029..16525
                     /locus_tag="IYA83_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ8586179.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16606..17289
                     /locus_tag="IYA83_000728"
     CDS             16606..17289
                     /locus_tag="IYA83_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586180.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17934..19124
                     /locus_tag="IYA83_000729"
     CDS             17934..19124
                     /locus_tag="IYA83_000729"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ8586181.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19226..20179
                     /locus_tag="IYA83_000730"
     CDS             19226..20179
                     /locus_tag="IYA83_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ8586182.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20248..21597
                     /locus_tag="IYA83_000731"
     CDS             20248..21597
                     /locus_tag="IYA83_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8586183.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21780..22103)
                     /locus_tag="IYA83_000732"
     CDS             complement(21780..22103)
                     /locus_tag="IYA83_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586184.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22232..23395
                     /locus_tag="IYA83_000733"
     CDS             22232..23395
                     /locus_tag="IYA83_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586185.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23465..24814
                     /locus_tag="IYA83_000734"
     CDS             23465..24814
                     /locus_tag="IYA83_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ8586186.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24859..25290)
                     /locus_tag="IYA83_000735"
     CDS             complement(24859..25290)
                     /locus_tag="IYA83_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586187.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25678..28320
                     /gene="alaS"
                     /locus_tag="IYA83_000736"
     CDS             25678..28320
                     /gene="alaS"
                     /locus_tag="IYA83_000736"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ8586188.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28520..29116
                     /locus_tag="IYA83_000737"
     CDS             28520..29116
                     /locus_tag="IYA83_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ8586189.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29238..30386
                     /locus_tag="IYA83_000738"
     CDS             29238..30386
                     /locus_tag="IYA83_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ8586190.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30424..30837)
                     /locus_tag="IYA83_000739"
     CDS             complement(30424..30837)
                     /locus_tag="IYA83_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ8586191.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30916..31347
                     /locus_tag="IYA83_000740"
     CDS             30916..31347
                     /locus_tag="IYA83_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ8586192.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31446..31679)
                     /locus_tag="IYA83_000741"
     CDS             complement(31446..31679)
                     /locus_tag="IYA83_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ8586193.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31862..33532
                     /locus_tag="IYA83_000742"
     CDS             31862..33532
                     /locus_tag="IYA83_000742"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ8586194.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33679..34065
                     /locus_tag="IYA83_000743"
     CDS             33679..34065
                     /locus_tag="IYA83_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586195.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34052..34750
                     /locus_tag="IYA83_000744"
     CDS             34052..34750
                     /locus_tag="IYA83_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ8586196.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34755..35219
                     /locus_tag="IYA83_000745"
     CDS             34755..35219
                     /locus_tag="IYA83_000745"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ8586197.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35216..36127
                     /locus_tag="IYA83_000746"
     CDS             35216..36127
                     /locus_tag="IYA83_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ8586198.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36362..36901
                     /gene="pyrR"
                     /locus_tag="IYA83_000747"
     CDS             36362..36901
                     /gene="pyrR"
                     /locus_tag="IYA83_000747"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ8586199.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36915..38198
                     /locus_tag="IYA83_000748"
     CDS             36915..38198
                     /locus_tag="IYA83_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ8586200.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38211..39137
                     /locus_tag="IYA83_000749"
     CDS             38211..39137
                     /locus_tag="IYA83_000749"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ8586201.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39156..40436
                     /locus_tag="IYA83_000750"
     CDS             39156..40436
                     /locus_tag="IYA83_000750"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ8586202.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40441..41517
                     /gene="carA"
                     /locus_tag="IYA83_000751"
     CDS             40441..41517
                     /gene="carA"
                     /locus_tag="IYA83_000751"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ8586203.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41522..44707
                     /gene="carB"
                     /locus_tag="IYA83_000752"
     CDS             41522..44707
                     /gene="carB"
                     /locus_tag="IYA83_000752"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ8586204.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44761..45537
                     /locus_tag="IYA83_000753"
     CDS             44761..45537
                     /locus_tag="IYA83_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ8586205.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45534..46466
                     /locus_tag="IYA83_000754"
     CDS             45534..46466
                     /locus_tag="IYA83_000754"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ8586206.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46463..47179
                     /gene="pyrF"
                     /locus_tag="IYA83_000755"
     CDS             46463..47179
                     /gene="pyrF"
                     /locus_tag="IYA83_000755"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ8586207.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47186..47815
                     /locus_tag="IYA83_000756"
     CDS             47186..47815
                     /locus_tag="IYA83_000756"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ8586208.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47803..48117
                     /locus_tag="IYA83_000757"
     CDS             47803..48117
                     /locus_tag="IYA83_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586209.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48155..48796
                     /locus_tag="IYA83_000758"
     CDS             48155..48796
                     /locus_tag="IYA83_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586210.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48827..49531
                     /locus_tag="IYA83_000759"
     CDS             48827..49531
                     /locus_tag="IYA83_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ8586211.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49528..50430
                     /locus_tag="IYA83_000760"
     CDS             49528..50430
                     /locus_tag="IYA83_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8586212.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50588..52294)
                     /locus_tag="IYA83_000761"
     CDS             complement(50588..52294)
                     /locus_tag="IYA83_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ8586213.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17146 a         9140 c        11269 g        14894 t
ORIGIN      
        1 ggaacgagtc ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca
       61 gatgaacaag gatctcaaac agttgctgat cctcaagcgt ttatcgatca aatcggctgg
      121 tctgcgtcag aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg
      181 ttgttggaaa gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc
      241 ggtgtgaaag gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaacg
      301 gacaatgggt aacgatgaat gaaccattcc gaaaatattc ttcttattgg gaatccttcc
      361 aagaccatgc gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg
      421 gcgtgtggaa aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt
      481 atgctaccga tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa
      541 cccgttttga tacgccatca acaggttcgg ctaccacaag tacaacaaca acgactacta
      601 cgacgacttc aacgtcatca tcttcaacaa cagatgtaaa tacaaataat ggtcagacat
      661 atacagtcgt gtcaggtgat acgctatggg atattgcaca aaaatttggc atgtctgttg
      721 atgatctaat ggcgaaaaat ggcatgtcta tgtccagtta ttctttatta gttggtcaag
      781 aactaaacgt ttgatttaga taaaaaggca aagaaactac catatatggt atatctttgt
      841 cttttttttg ctagtcaaag acgaatcact cgccataaat gaacagattg tattatgata
      901 agtaagaaga gcaaagacaa ggaggaattg aagatgacag ctatctataa ctcagtcacc
      961 gaattgattg gcaaaacgcc aatcgtcaaa ttaaataaaa tcgtaccaga agattcagca
     1021 gatgtttttg taaagcttga gttttttaat ccaggaggaa gtgtaaaaga tcgtattgct
     1081 ttaagtatga tagaaaaagc agaacacgat ggtctactga aaccaggtga tactatcatt
     1141 gaacctactt ctggaaatac aggaatcgga ttgtcgatgg taggagttgc aaaaggatat
     1201 aaagtaatca tcgtgatgcc tgagacgatg agtatcgaaa gacgtttatt gatgaaaggg
     1261 tacggcgcag agttgatttt gactcctggt gcggatggta tttctggatc aatcagagaa
     1321 gcagaacgtt tggcaaaaga aaacggctac tttttaccat tacaatttga aaatgaagca
     1381 aatcctcttg tgcatgagaa gacgactggt cctgagatac atcaagcatt tggagtaaat
     1441 ggcttggacg cgtttgttgc aggtatcggt acaggaggaa cgatcactgg cgctggccga
     1501 gaattaaaac gtgtgtatcc gaaaatcgaa ttaatcgggg tagaaccagc agaatctgct
     1561 attttagaag ggaaagaagc gggacctcac aaaatccaag gaatcggtac aggttttgtt
     1621 cctaaaacac tggatacttc tgtgtatgat aaagtccttt ctattagtgg agacgaagcg
     1681 atggaaacag ctagagaagt tgggcgaaaa gaagggattt tagtaggcat ctcatctggc
     1741 gctgcaattg ctgccgcgct aaaagtcgca aaagaattag gaaaaggcaa gaaggtatta
     1801 gctgtggttc ccgacaatgg tgaacgttat ttatctacag ccctttacca agagatataa
     1861 ggcacttggt ttttctccag ataggtttat gtaaaaaact tttaaagatg aaaagaacac
     1921 acaaaaattt atgatgagaa aaattatttg aatagagacg taaaatagtg aacgcgactg
     1981 ttttacgtct tttttcatta aaatacaata aaattgaatt ttcttttcat gttaagtgga
     2041 ttttaaaatg taaaaatagt atgataagac gagaataaca acgtggggtg tttacatgga
     2101 aaataagtta gtcgaagagg ttatcgaaaa gttaaaaaaa gagaacattc gaatcacacc
     2161 tcaacggtac gcggttttag aatatttgat tgaacatcat tcccatccaa cggctgatga
     2221 gatttataaa gcacttgaac atcggtttcc aaatatgagt gttgcgactg tttacaataa
     2281 ccttcgctta tttacttcga ttggatttgt tcaagaaatg aagtacggcg atgcttctag
     2341 ccgatttgat ttcagttcaa agcgtcatta tcatgtaatt tgtgaaaact gcggtaagat
     2401 tgttgatttt cattacccag ggcttgaaga tatagaaatg gctgcgagca gactgactga
     2461 ttttgagatt aatgaacatc ggttggaatt gtatggactc tgtccggatt gtaaagagtc
     2521 agcaaaaaag gcgcaaatgt aaaaacagtt tctataagtg ttatacctaa aaaaagctag
     2581 ctgtgtaaaa aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatatc
     2641 aaagtgaata ttatcacaat aagaatcttg tagatattgg gttcttatgc ttgaaaagga
     2701 atgaaactac aaacaaacct ttgtgaattc acaagcatta aactgttgta tccgttttat
     2761 ttctgttata gtagttttgt cgaaggacaa aatgttaatc ggagggacca taaatgaaag
     2821 tagtagtaat tggctgtact catgcaggta ctgcagctgt aaaaagtatt ttaaaaaatc
     2881 atccagaagc agaagtgaca gtatatgaaa gaaatgacaa tatttctttc ttatcttgcg
     2941 gtatcgcact ttatgtcggc ggagtagtaa aggacccagc aggcttgttc tactcaaatc
     3001 ctgaagaatt atcttcatta ggagcaacag ttaaaatgga acacgatgta acagacgtcg
     3061 atacagaaaa taaaacagta acggcaaaag atctgaaaac aggagaaaca gaaacggttt
     3121 cttatgataa attagtgatg acaactggtt catggcctat tatcccaccg attagtggaa
     3181 tcgatgcgaa aaatgtactc ctatgcaaaa actacaatca agcaaatgaa attattgcac
     3241 aagcgaaaga agcaaaacgt gtagtaattg ttggtggtgg atatatcggt attgaattag
     3301 tagaagcttt tgtcgaatct ggaaaacaag tgacattgat tgatggatta gatcgcatct
     3361 tgaataaata tctagataaa ccattcacag atatcttaga aaaagaattg acagaccgcg
     3421 gtgttactct tgcgctaggc gaaaatgtta ctgaatttat cacagatgaa gaaggaaaag
     3481 tgaaacaagt agctacacca acagatacat ttgatacaga tatggtcatt ttatgtgtgg
     3541 gcttccgtcc aaatacaaaa ctgatcgaag gaaaagtaga tacactgcca aacggggcaa
     3601 tcaaagtgaa tgaatacatg cagacaagtg atccagacat ttttgcagct ggagactctg
     3661 cagtagtcaa ctacaatcca agtggcacgc aaaactatat tcctttggca acaaatgccg
     3721 ttcgtcaagg tttacttgtg ggaaataact tgacagatca taaaatggcg tatcgtggta
     3781 ctcaaggaac ttcaggactt tacctatttg gctggacgat cggttcgaca ggtgtgacca
     3841 aagaaagcgc aactttgaac gatctagaag tccaagcgac tgtatttgaa gataattatc
     3901 gtccagagtt catgccaact actgaaaaag taatgatgga gctagtctat gaaaaaggaa
     3961 cgaatcgtat cgtaggtgct cagttcatgt ccaaatacga cattacacaa tcagcaaata
     4021 ccatgtcttt agctgttcaa aataaaatga ctgtagaaga tctcgcactt tcagatttct
     4081 tcttccaacc acattttgac cgcccatgga attacttgaa cctacttgca caagaagcgc
     4141 taggcgaaat cgaaactgca caataaagaa aaagggttgt gacacaattt tgtcacgacc
     4201 ctctgtcttt ttttgtgaaa agtacaatta gttgtctttc ttcgaatttt ttgtcacaat
     4261 aagcagcaaa gataaagctg ttacataaaa gaacaagcag ctttatttga ttttgaaaag
     4321 aaaggtgaga aagaagatga ataaagaata ttttgtccaa caagatgcat ttttagaaga
     4381 ttataaaagt gcttcgaaga aattaacgat caatattgat gagttggcag atcaagtact
     4441 ctctcagttg aatgaaacgc aagaggaata ttacacgatg ccagcaaatg acgccaaaga
     4501 taatgtttct catcgttttc ctttcagtag acgcatctac tcagatgaaa atgacggcac
     4561 attgaataca gagtacacat atgaaggtca gccatatgtg ctagaaactg atgatgatct
     4621 ttaaagacag tttttgaaaa ggccgctcat ccttaagtga tgacaaaaaa tcatcacaat
     4681 taccgatgac tgtctcttta ctatctttta atcagaaaaa ggggttatga caagctttgt
     4741 caaccccttt ttttcttggt ttcagattgg gttttcctac aaaatatttt tatctttgtt
     4801 gaataggaaa caaaaaaaca atataatgaa agaaaaagaa ggtgtagaaa tggataaaag
     4861 acaactgcta atagaagaat atctggattt agctaatgag attattcatc gtaataaacc
     4921 agaaatggaa aaagaatttg caggatttac tctaaatgaa ctagaagtga ttgaacatat
     4981 cgggaaaatc cctgatccta acgtgacaaa attagcgaat gctagttaca tgactagagg
     5041 agccatcagt aagctaacaa aaaaactgat agctagaggg atcataaaca cttatcaaag
     5101 cgaagagaat aaaaaagaaa tctattttca attgacgcca atcggacaaa aaataaggaa
     5161 tagacacgag caactccatc aaaaatgggt acagcgagat gctactgtat ttgaaaatat
     5221 gtcaaaagaa gaatttgaca cagtatttcg ttttatcggt cgatacagag aatttatacg
     5281 aagtagcaaa tgaagctact attttttatt gtagtgataa taggcaaaaa tcataagcaa
     5341 aaatccgaat accgtcggaa gaaatggtgc tgcagcataa agctgtcctg cgattagcgg
     5401 gccgatgacg cgcgatagag attgtatact ttgggtaccg cccattacaa gaccttgctg
     5461 gttatctgag acagaattgg atagttggcc attgaataca ggagtaaaaa tagaatcacc
     5521 gaaaccataa cagataatcc caaataaaaa caaaatcggc agtagaagaa ttccagataa
     5581 tgtgatgaat agataggcga ggatttcact gaacatccct attcttgtga tctgctgttc
     5641 tgaaaatttt ttgagcaatc gaggcataat gaacaattgc gagacagtat ccaagattcc
     5701 aatcaaagag aagcttaaac caaccaaccc agccttccac tgaaaggcgt caatcgaaaa
     5761 ttgagaaaag atagattgaa gagagccagc tgccaaccaa acagcaaaac ctgctgtcaa
     5821 taaaggaata acggatgaaa tcttgaataa ttgttgtaat tggtgaaaag gtcttacatg
     5881 ggagaaactt aaatctactg aacgtttttt tattggaagg ctttctagca taaatgtgta
     5941 gccataaacg gcatttaaga aagtaaagag tgctccaata aatataggaa cagaattccc
     6001 caactcagct aatagacccc ctataatagg acccagcgtt gttcctatac cgactaaagc
     6061 acccatccat ccaaaaactt tggttctttc attaggttcc gtgatgtcgg caaagtaagc
     6121 atataaggtg gagatttctc ctgccgtcaa tccttcaatg atacgaccaa ggaacaacat
     6181 ccatatcgaa tttcctaaac caaaaagcaa atatccgata cttgatccta ataaactgat
     6241 aataaggatc ggatgacgcc caaaatgatc acttaagcta ccgagaatgg gggcagataa
     6301 gaaggcagct agagcataaa cagacatgag caaagtgata tagaaagctt gctgatgtat
     6361 ggagtgagaa taaggcagcg ttaaaaacgg aagtacagga ctgacgatag taaatcctaa
     6421 tccagtgaat aagacagaga cgtatccaaa aactttagaa tgtttatttg tgaaactgta
     6481 catgtaaaaa ctcctttatg caataatgtt aacttggaaa caaaaatagt gtatagtaat
     6541 tttgtttcct tgtcaacatt ctaaggaaca gcgaaatcta cttaatttat ttattccgaa
     6601 aagtgaagat acgcatcaaa aacatgaaca aacaaaaatc aagtagacga cattgtaaat
     6661 ctgaccgatc agtgaagtaa atagaaaaga aacctgttct tgcttcccat gtttttttta
     6721 cgtatactgt gattgagcgg aggagaaatc atgttgaatt tagaaaatta cttaagcgct
     6781 ggacaaacag tcatatctaa tcttttactt aaaaattatc ataggatcgg tctacaatct
     6841 gaagaatttt tatttgttct acagttgcat atggcacagc ttgaaggaga tactttccct
     6901 gatttgcaga tgattgctat ggatatggga atcaaacaag atcagatttt tcaaatactg
     6961 gatcgtctcg taacaaatgg atttatcaag atagaaacga cgattgtcaa cggtaaaaaa
     7021 gcagatcgat ataatcttta ccccatctat gatctattag gtgagtattt aaaaacacaa
     7081 gaaaaaaaat acgaagaaaa aacacaagaa aaagaaatcc agtcagtgta tcaattattt
     7141 gaacaagaat ttggacgccc gctttcttcg atcgagttcc agcggatcgg acaatggcta
     7201 gaagaagacc attatcagcc ggaaattttg aagctagcac tgcgggaagc cgtattaaat
     7261 caggcttata gttttaatta cgttgaccgc attttgctat cgtgggaaag aaaaaatcta
     7321 cgtacaaaac aacaagtgga agaagaccaa aaacgaagaa aacagcagct tcttcaaaga
     7381 gaagtagaac agtcagctca acaagaagaa ttgccgaaag tctcactgaa aaattggttg
     7441 gaggagtagc ggaatatgtt aagcaaacaa aaaacaatgg aagctctaga aacgatgtat
     7501 ggaatgtttc ccgaagcaca tggagaactc aagcacaata atccatttga gttattgatt
     7561 gcagtgatct taagtgccca ggcgacagat gtatcagtga ataaagcaac gccagatttg
     7621 tttgcctctt ttccgacacc cgatgcgtta gcagaagctt caatcgatga aatcattctt
     7681 aaaataaaaa cgattggttt gtatcgaaat aaagcaaaaa acatcaaagc gtgtgcacag
     7741 cagctgattg aacgtttcga tgggcaagtg ccaacgtcaa gagaagaatt gatgtctctt
     7801 cctggtgtag gcagaaagac agcaaatgtc gtattggggg atgcattcgg gataccagca
     7861 atcgcagtag atacccacgt ggaacgtgtc tcaaaacgcc tgaggatctg caaactagat
     7921 gctacggtca tggaagtaga agaaacactg atgcggaaag taccacaaga attatgggta
     7981 aagacacatc atacactgat tttttttggt agatatcatt gcacggcaag aaatcccaag
     8041 tgcgaggtat gtcctttgct atcaatatgt caagatggga aaaatcggat gcgtctaaaa
     8101 gaaaaggcgc taaaaaagaa accacgcctg tagacgtggt ttctttaatt gcaaataaat
     8161 gtatgaatta tcttgttatt atggagaaaa gctgtttcgt ttatcttttt tgcatgaaaa
     8221 agtaaatgta catactcagt agctaggatg gtaacttatt acaatttatt gcgataaaaa
     8281 taaaagttgt tatttttatt gggaaaatac ccgatgtaga aaataatgcc ttccaatata
     8341 cctataaaat aagtccagta ttccaaagga ttatataaat aagtgacgta tctatctaaa
     8401 ggtgtaataa aaatgataag tgataataaa aaaataccat ttataactaa acataaacta
     8461 atggatttgg gcggtttcag gctcatcaga aaaatataga ttctggtgaa caacaaccat
     8521 aaaaaagtga gaatcattgc atgagagctt ctttccataa attttccttt aagagtagtg
     8581 cttaaaagta cagaaaaaat aataaatgca aggataggag caattaaagt aaatttattt
     8641 aataataaac aaaatttttc agctttttta ttcttggaat aacgaggtat tgttttcgat
     8701 aaaaaaagat ggtgcagaca aattatgata attaagcata ctatcagata actcaaaatg
     8761 aagccttctt tcaaaaaatt attcaaaacc atatattgta ttttttattt gataataaca
     8821 atgggattat taagaagtgg cactaaataa aagactagtg tgtatttagc cgatttaatt
     8881 ataaacaagt aaatggataa agtatatgta atgataaaag aaaaatggat tttctaattt
     8941 ttaagctaaa tgttagatca cacatgtata tgctggatag tcttataaaa aatcatgtaa
     9001 tacagcttgg tacatgattt ttatttgtag ctgtttttta catagacaca aaaaagaaac
     9061 cacgcttgta gacgtggttt cttttttact atctgtttcc agaagctgag gtgctgctgg
     9121 atggtgtatg gtcatttgca gcatccgctc cgctagttgc tggctcactg gaagaagcag
     9181 gaggagcaga tgaagacggt gtcgagcttt cttcggatga agaagaagga accgttgttg
     9241 aagacgctgg ctcgcttgtt tctgctgtcg tgctttcttt tgaggactca gcagttgatt
     9301 cgctttgact agtagagcta gagctttgag tcgttgtctc tgttgttgag cttccaggag
     9361 tctggacata gctggaagat ggaatagttg ttgatggtgt aattgcattc gatctagctg
     9421 tatattgatc tttgtagtac agttcaccgc ccacacgaat taatccgctt ggcatctccc
     9481 agtctgtatt tgtcacgtta gctgaaacat actgcataag ctcgcgataa acatcagagg
     9541 ctacgtgtga cgattctgaa gtgacaggtg tcattttttt attgtaacct gtccacacgg
     9601 aaatcgaata atttggcgta tagcccgcaa ataggatatc tggatacacc ccagaagaaa
     9661 taccaagttt tgcatactcg tcatctgtgt aattggatgt tccggtctta ccggcttgat
     9721 ataacccagc gatttgcgca ttcgtaccag ttccttcagt gatcgtatct tttaaaatgt
     9781 ctgtgatcat ataagctgtt tctggagaca tggctgtttt tccatctggc tcataagttt
     9841 cttctgttcc gtcttggaag acgattttat taacatattg aggtttgtaa taagtccctc
     9901 cattggcgaa agccgcatag gctgctgcca tttttaagga agaagctccg tattttgtcc
     9961 catcttgttc ttccgtatta ctagagatcg cattcgattg atggatcgta ctgtattcga
    10021 tcccaagatt ttttaagaaa gaagccactt tatctgaacc gacttcattg aataatttga
    10081 ctgctggaac attacgggat aagtaaagtg cttggcgtaa agtaatggtg cccatatatt
    10141 gattatccca gtttcctaca ggagtcgaag tcccttcata gttatacggt gcgtcagtaa
    10201 tagtttttcc agtagaatat ttcaaatatt caaaagctgg tccgtaatct gtgacaggtt
    10261 tcatcgtaga accaaaatca cgggaagtat tcactgctaa gttgttgcct aaagttacgt
    10321 cttctgcgat gtgacgtccg ccaatctggg cttttacttt cccggtattg gtgtcgataa
    10381 gggtggaggc aacttgcatc tcgtcgtctg ggtaagagac atactgatcc gtgttgacga
    10441 tatcatatag tttcttttgg gcgtccagat ccaaattcgt ataaatctct aaaccgtctg
    10501 tgtagacatt cttatctgtt ttttcttgta cttcattgat gacttctttg acataattat
    10561 cgacgatctt agtattgtca tcggattgag ttagttcttg cagaccatcc gttaccggta
    10621 cattaacagc ttgatcatat tctgtttgag agattttttc gttttgaagc atcgtataca
    10681 aaacagtatc tcgtcttttc ttcgcctgat ctggataaac ataaggatca taggcggaag
    10741 gggcttgagg catcccagca agcaatgctg tttgcggtaa agaaagttct gataatttct
    10801 taccaaagta catctcagaa gctgtctcca tcccgtacaa gccgtttgac atatacactt
    10861 tatttacgta gtaggtcaag atctcttgtt tcgatttttt ttgttccaat cttacagcca
    10921 tccacgcttc ttgggccttg cgtttcagag tctgatcctc ggcactggtg gagaagaagg
    10981 aaagtttgat caattgctgt gtcaaagtac ttccgccttg cagtccgcca gaggtaaagt
    11041 tagagagggc agaaccgatg atccgaatag ggtcaacccc gatgtgttta taaaaacggc
    11101 gatcttctac cgacacgatc gcatcttcta atgttttcgg taattcattc gcagaaattt
    11161 tttcacgctt ttctgcgcct aaatcttcga atagttcgcc atcttgtgta tatagtttgg
    11221 aagatacggt agcgtctaat tttttatccg tcaattcggg cgcatctttt gcgtaatacc
    11281 agaaaagtcc tacacctgct aaaaagagga tacagaaaaa tgagagtatt cctaaaagga
    11341 tcttgataaa taatcctttt ttatgtgttc cagaggactt tccagaacct ttgccagaat
    11401 tcttcttcac actcttttta ggagtaggct gtttctgtcg tctagagctt ctagtttgtt
    11461 catttgccat tcttgctttc tccttcaata gataatgttg ataaatagaa gtcaaccgcg
    11521 tccaaataag gaatccgcgg agaaatacca gttttgatct ctattcctag ttcttcgatc
    11581 aaagataaag gtagtgattt gcgtcctttt tccttttggg cgtcccagta agcaatcaaa
    11641 tgaacactat tcaaataaaa acagcgcttc aaagacgaaa accataacaa gacaaagcag
    11701 accccatttt gttcgaggca tgctttcata tgttcgattt gatgggcatg gaagttttta
    11761 aagggaaaag atgttttgtt cttggtttct tttgcttcaa agtccaagta aaagcctcga
    11821 tagacgccat tatagtctgt agttgaggct tgggtaaagt aggcttcctt gatcacagca
    11881 gcacttctct taggatagtc tactttcacg atttggatag gggtaggctt tttatggata
    11941 accgccaacc cgcgggaaag ataatattta ttactttcat tgattgcttc ttcaaagcgc
    12001 atcccccgat tgccgaattc agtgatctga tttctttttt cttgaatttt ttcgacggga
    12061 gaggcattct tataataagg ttggccattt ggatagcgca ataccatctt taatcacact
    12121 cctaggccac attatagcaa aaaagcggat agaaagaaaa ggaatctgag atggataagt
    12181 caaaagtaat ctatgtgtct gggtatcgaa gtttcgaact cggtgttttt aaagaaaatg
    12241 atcctaaaat ccaagttgtt aaaaatgttt taaaaaaaga actccatcag ttagcagaag
    12301 aagggctgga atgggtactt gtcagtggga atttgggcgt cgaactatgg gctgtgcaaa
    12361 tagtagcgga attgaaagaa gaatatccgg aactatctct tggtattttg tatccttttt
    12421 ctgattttgg taataactgg aatgaaggga accaagaaaa aaaacaatta gcagaacaat
    12481 tagcagatta tgtcgaagct gttagccatc aaccctacca atcacctagt cagctaaaga
    12541 atcacactcg ttttatactt gagcatacag tgggctgtct gctgatctat gatgaagaat
    12601 tccctggaaa aacgaagtat ttttttgaag atgcacagaa ttaccaagca gatcatccct
    12661 acgaaattcg gctgatcagt atggacgatc ttcaaaattt tagtgaataa tcgatattgt
    12721 gtttgattta tcaacttttt tcctatacaa tgaaaaaggt agaaatgtta ttacgtaaat
    12781 gaaaactaga ggtgtaaata tggcgaattt ggtttatagt cctaaagaca ttttacaaca
    12841 agaattcaaa acaaaaatgc gcggctatga tcccgttgaa gttgatgaat tcttagacaa
    12901 tatcatcaaa gattatgaaa cttacagcaa agaactcctt gctttacaag aagaaaacga
    12961 tcgattgagt gcaaaagtag ctcaattatc taaaactcaa ggagcagctc aaacacgcgt
    13021 gcaacaaaca gaggcaccaa agagcgcagc agtgaccaat tttgatatct taaaacgctt
    13081 gtcaaatctt gaacgtgaag tatttggtaa aaagcttgat caacaagcaa gtgcagtcaa
    13141 accagcacaa cctaatccaa ataactacac aaatgcagac acaagcttag atgataatga
    13201 aaaaacacgt caattttaat ttttgattaa tgaatttgcg gttttcgggt aatcgcggtc
    13261 tgacttgatc aggctgagga aagtccatgc tcgcacaggc tgtgatgcct gtagtgttcg
    13321 tgcttagcga aaccataagc taaggtactc tttggagtaa cggcaggaaa acaagctaag
    13381 gcttcggcta tgcttaagta tccttgaaag tgccacagtg acgaagcggc tggggaaacc
    13441 catccggtgg aacgcggtaa acccctcgag cgagcaaccc aaacaatggt aggggcgctt
    13501 tttgtctgga agaagaacag gacaaaaagg cagtataact gcagatagat ggttaccgct
    13561 ttgttttttc tccctggaaa agcaaagtac agaacatggc ttatagaaaa ccgcaaacat
    13621 cagggaatta ggtcgtgaca aaagtaatgc gttataagaa acaagaaaaa catttgaaag
    13681 ctatttttca atacttcaga tgaatcgttc ttattacaaa acaaactttt gtatcaccgg
    13741 ttattcggaa tttgagtaaa agtgtggggt aggactgatg tcccacctcc tttttttata
    13801 aataaggaga ttgtgtatgg aaaaaaacag gaagttcaac ctcgtcgcaa ctgcggcaag
    13861 cgggctagaa gcactggttg gtaaagaatt aagagatctt ggaatcgaat gccaagttga
    13921 gaacggacgt gcacgttttg aaggaacaat ggaaacgatc gctacagcta atttatggtt
    13981 acgtacagca gatcgagtaa aaatcgtggt tggggaattc gatgcgtata cgtttgatga
    14041 attgtttgaa aacgtaaaag cattgccttg ggaagatttt cttccactag atgcagcttt
    14101 tcctgtagct ggaaaatcga tcaagtcgaa actatatagc acacctgatt gccaagcgat
    14161 cacaaaaaaa gcaatcgttg aacgtctccg tacctattat caccgtccag cttctgtacc
    14221 actgacggaa actggcgcac attttcagtt ggaagtagct ttgctgaaag accatgtgat
    14281 ggtaactctg gatacgacag gaccaagttt attcaaacgt ggttatcgct tagaaaaagg
    14341 tggagcacca ttgaaagaaa acatggcagc agcacttatt atgttgacaa attggcgaaa
    14401 agaccgtcca ttttatgatc ctgtatgtgg ttcaggtacg ctttgtattg aagcagcctt
    14461 aataggccat aatatcgcac caggattcaa tcgtgagttt gcttgcgaat cttgggattg
    14521 gttcagtcaa gaaatcatgg aaaatgttcg atcagcagcc gaagaaaaag cagattatga
    14581 tattgaatta gatattactg gttcagatat caatggacgg atgattgaaa ttgccaaagc
    14641 caatgcagaa gaaatcggat taggctcttc gattaccttt aagcaacaag cagtcaaaga
    14701 cttcaaaacg gataaagaat acggggtcat cgtggctaat ccgccttacg gagaacgtct
    14761 aggagaagaa gaaactgttc ggaaattata taaagaaatg ggcgatgtat tccgtccatt
    14821 aaaaacatgg agtaaatata tcttgactag cgacttagcg tttgaggaat tttacggaca
    14881 aaaagcaacc aaaaaaagaa aattgtacaa tggtgcattg cgtacggatc tattccagta
    14941 ttggggaact cgtccgccta gaaaaaaaga gaaagacgaa taatcttact aattgattca
    15001 ttcaattgga tgtttggagg aatagggaat gaaagaaaaa gagtttttac aagaagtaaa
    15061 agaaatcaat cttctacaac aagctttagg aatcctcgat tgggatacac aaacaggtat
    15121 gcctgaaaaa gctagtgcat accgaagtga agtagacagc tatctttaca gcatttattt
    15181 ttcaaaaaaa atcggtccaa aaatcaaaga agccattcgc tatttttctg aacatccgga
    15241 agaattgtcg gaagttggaa ctgccgtttt tgccaaagta aaagaagagt atgatttgga
    15301 acatcgcgta ccggaagatc ttatgagaaa atatacagct gccgtttctt cagcacacgg
    15361 acaatggcaa aaagctagag aaacacaaca attttctgat tttcaacacg cattgaatca
    15421 aaatattgaa ttaacaaaac agttgatacc ttactggaga aaagatgaaa agacgaatta
    15481 tgatgttttg ctgaatcaat acgaacccgg gatgactgtc gaaatccttg ataaggtatt
    15541 tgatcagctt aaagaaggaa ttctttcgat ccgccgtacg ctccaagaaa aagggactga
    15601 acctgaaaca gactttttaa atcggagaat gacgaaaaaa caacagaaga gatttgtagt
    15661 gaaagtaatc gaacaacttg gttatgattt tagtcgtggt cgtttagatg acactgtcca
    15721 cccatttatg actggaatta atccgaatga tgcacgaatc acgacacgat ggaacgaaac
    15781 agatttcaaa atggctgttt ttggagtgat ccacgaggct ggtcatggta tgtatgaaca
    15841 agacatcgat gataaatatg cgtatacacc ggtttatgaa ggtacatcaa tgggtatcca
    15901 tgaatcacaa tcgttgttta acgagatcat tattggaagt agtcgcagct tctggaaaaa
    15961 gcaatatcct ttctttcaag aatgtgcaga aggaacattc gatgatattc cttttgacaa
    16021 attttatcgt ttattgaacc atacaaaatc aagtttggtt cgtatcgagg cagacagttt
    16081 gacttaccct ctccatatca tcattcgtta tgaaatcgaa aaaatgattt tcaatgaagg
    16141 ggtagatgtt gctgaactac ctgaaatatg gaatcaaaaa tatgaagagt atctgggaat
    16201 acgaccaaca aatgatgtgg aaggcatcct acaagacgtt cattggtcag gtgcaagctt
    16261 tggttatttt ccctcttatg cattagggta catgtatgcg gcacaattac ttcattcttt
    16321 gcagcaagaa atagatgtgg aaaatgttct agcgtcagac gattattctc caatcaaaaa
    16381 ctggatgtca gagcatatcc atcaatatgg tgcatctaga aaaccaaatc aattaatcat
    16441 ggatgcaaca catgaaccgc ttaatccgca atacctgatc gactatatga aagacctgta
    16501 ttttaatgta tatcaggtaa aataaaatac aatttacaaa gatagctgaa attcatctaa
    16561 tctatagatg ttttcagcta tttacttaag aaagaagtgg aacgaatgga taaattacaa
    16621 gtgatcgctg attatagtat ggaccaatta gcgcatgatc aaacaggaca tgggagcgat
    16681 cataccaaaa gagttgtcaa gttagctgaa aaaattttgg agacggaacc gcaggcagac
    16741 cgctttgtga cgcttgcagc agcttaccta catgatacga ttgatgataa agtcgttaaa
    16801 aatgagaacg aggcaaagca acagcttcgt gtattccttc gctctttgcc aatagtagag
    16861 gaacagatct cgatgatttt ttccattatc gaaaatatgt ccttttctaa aaatctctct
    16921 gaagcggccg aactatctct ggaaggtaag atcgttcaag acgctgatcg tatcgaagca
    16981 ttaggagcaa tcggcatttt gcgtactgct tatttcggcg gtggacatgg gcacccgatt
    17041 tttgatcctg aactgtatcc gcaaacattc aaggacaaag aaaattaccg aaaaggcacg
    17101 acagtgatca atcattttta tgaaaaactg ttcttgctgt ctgataaaat gaatactgat
    17161 tacggatatg aagaagccaa acgaagagaa accttcatgc gtgaattctt agaagaattt
    17221 tttctggaat ggtctattga cgatgaaaat tttactccag acaattggat caaaaataac
    17281 aaaaaatgat ggaggttgtg acagaagtgg tcagctccaa gatatttcgg atcattttcc
    17341 gtaaaagtta cttctggaac actgtttacc aagaggtcgt gagcctgccg ttcagctccg
    17401 agtcacaagg aacaatttca aaaaatagct tctcgtgttt gatgaatatt gtaacttgtg
    17461 attcggaatt ggcaggagaa caccgtttat tcggaagaat aggactgtga caagactttt
    17521 gtcacagtcc ttttttcgaa ttcaacaagt aaaactttta agaaatattc agagagtaaa
    17581 attaaaaatt gctgataaac gaagatggaa attttatgta tttttattgg tctatgcaat
    17641 aaataaatta aatattcaga atacaattga ttttaacgaa agggcttcta tcaatggttg
    17701 tgttgtattt atctgcccag gtagagtaaa atagatcatt atctaaaagg taattgttca
    17761 tactatataa gtcatttgaa agtaaaaaga tactgtaaat aaaaaatgtg gttaaataat
    17821 tctcataatc agtgtaagcg ttatcaaaaa tatagcatac ttgctttgta gatgaatgac
    17881 acgatatatg tttggtatgt tctgcaagac agatgaatat ggaggtggag acaatgtctt
    17941 ccaaagaact aacaatgtat ttagagaaag aattggatca actgaaagaa aaggggttat
    18001 acaatacgat cgacatacta gaaagtgaaa atggtgcttg tatcatcgta gacggaaaaa
    18061 agatgatcaa tctagcatcc aacaattatc ttggatttgc caatcgcgaa gaattgaaaa
    18121 aagcatgtat tgaagcaacc gaaacttatg gtgtaggagc aggagcagtc cgaacgatca
    18181 atggttcgct gaaaatccat cagcaattag aagaaaaaat cgcagaattc aaagggacag
    18241 aagcagcaat cgcctttcaa tctggattca actgcaatat gggcgctatc tctgcagtaa
    18301 tgacaaaaga agatgctatc ttatcggatg aattaaacca tgcatctatt attgacggtt
    18361 gccgcttatc cggagcaaaa atcatccgaa ttaaacatca agatatgaaa gaccttgaga
    18421 aaaaagcaaa ggaagcgata gaaagtaaga aatataaaaa aatcatgtat atcacagatg
    18481 gtgttttctc catggatgga gacattgcac gattgccgga aattattccg attgttgaaa
    18541 aatatggact gatcacctac gtcgatgatg cgcacggctc aggcgtaact ggaaaaggtg
    18601 caggaaccgt caagcatttt ggattaagcg acaaaatcga tatgcaaatg ggcacgttat
    18661 cgaaagctat aggtgttgtt gggggttatg tagctggatc gaaaacacta atcgactggt
    18721 tgaaagctag aagtcggcca ttcctatttt ctacttcgct aactccaggt gcagctgcag
    18781 ctgcattggc ttcgattact ttgatgcaag aacatccgga acttgtcgaa aaagtttggg
    18841 aaaatgctaa ttattttaaa gaagaattga aaaaagtagg ctataacatc ggtatgtctg
    18901 aaacacctat tactccagtc attttgggag acgaaaaagt gactcagact ttctctaaga
    18961 agctgatcga acatgggatt tatgctaaac caatcgttta tccaactgtg cctcttggga
    19021 caggcaggat cagaaatatg ccgactgcgg aacatacgag ggaaatgctt gatgaagcag
    19081 tagcagttta tcaaaaaata ggaaaagaaa tggaaattat ttaactaaat ttagtttttt
    19141 cgtctattta aaattagaga acatgttcac gcatatattg ttaatcatct gaagatagta
    19201 gtaaaaaaga tcagggagga tcatcatgaa aaaagtattg gtgacaggat gtttaggaca
    19261 aattggttca gagttgatca cacggctacg tcaagaacta ggcagtgaaa atgtcattgc
    19321 gacagatata cgcatgcctg aagtcattga gaaaaacgat atctttgaga tcctagatgt
    19381 aatggattac gaaaaaatgc gcagcatagc agaacaatat caagtggaca cattgattca
    19441 tttggctgct cttttgtcag ctgttgcaga agcaaaacca caattagcct ggaatttgaa
    19501 cgtgactggg ctggtcaatg cattggaagt cgctagagaa ttccagctga aattttttac
    19561 gccaagttca atcggcgcgt ttggtccaaa tactccaaaa gatcataccc cacaagatac
    19621 tcttcagcgt cctacaacca tgtatggggt aacgaaggta gctggagaac tactatgtga
    19681 ttattattat gtgaaatatg gcgtagatac tcgtggtgtc cgtttccctg gattgatttc
    19741 ttatgcaacc ttgccaggag gcggtacgac agactacgca gtcgatattt attattcagc
    19801 aatcaaagaa ggaaagtaca ctagtttcat tgatcaaggt acatcaatgg atatgatgta
    19861 tatgccagat gcgatcgatg ccattataaa actcatgaat gcagatcctg atcgattggt
    19921 tcatcgaaat gccttcaata tatcggcgat gtcttttgac ccggaagaga tcaaagcatc
    19981 tattcaaaaa atcataccag attttgaaat ggattatgat gtggatcctg tacgtcagtc
    20041 aatagcagaa tcttggccaa attcactaga tgtttcttgt gcgcaagagg aatggggctt
    20101 ttcgccgaaa tacgacttag atgtgatgac aaaggatatg ttggaaaaac tgaaagaaaa
    20161 attggtagag acggtataaa acaagaacca catccaaaaa gttttttata cttttgggat
    20221 tggctatggt atgaaagggg acaagggatg gaagaatatc aagccacgcc agtaaaagaa
    20281 gtaaaaatag aaaatgaatt aaaacgtacg atgggatttt ttactgcttt atcgactgtt
    20341 atgggcacag tgatcggtgc aggggtattt tttaaagccg caagcgtagc agaagttaca
    20401 ggatccgcca gtttgcatat gttttcttgg tttctcggtg gtatgatttc tgtctgtgca
    20461 ggactaacag gagctgaact ggcagctgct attccagaaa caggagggat gattaaatat
    20521 atcgaacgga tttacggaaa tacagcagct tttttattgg gctgggcgca agtcgtgatt
    20581 tattttccag caaatgtagc agccttgtcg atcattttcg gtacacagtt cgtcaatcta
    20641 tttggtcttt cccagtcaat gattgttccc gtagctgtta cagctgctgt atcgatcctg
    20701 ttgatcaatt ttttaggttc taaagctgga ggggcttttc agtcgattac gcttgtctgc
    20761 aagcttattc ctttgtttgt gatcgtcatt ttcggtttgt tccgacaaga aggagtagac
    20821 ttccaactgt ttccgatcca agcaggagaa aatctttcat ttttctcagc cttgggagcg
    20881 ggcttgctag caacgatgtt cgcatacgac ggttggatac acgtaggaaa tatttcggga
    20941 gaattgaaaa agccggcgaa agatttgcca aaagcaattt ctttaggaat catcggaatc
    21001 atgatcgttt atttattagt aaatgcggtg ttcttaagaa ccgcatctat cgacggagtc
    21061 gcaggaaaca gcaatgcggc aagtgacgtt gcaaaaatga tttttggcgg cttcggtgga
    21121 agactggtca cagttggtat cttgatttct gtttatggaa ccatcaatgg ctataccctt
    21181 actggaatgc gtctccctta tgttatggca aaagaaaaca acctgccttt tagcaagctc
    21241 tttgctaaac ttcatgataa aactaaagtt cctgtagccg caggtatctt agagctagtc
    21301 atcgctattg gcatgatgat gatcggtggt tttgatacac taacggatat gctgatcttt
    21361 gtcatttgga ttttttatac gatggtcttt gttggtgtca ttcttcttcg taaaaaagaa
    21421 cctgatttgt ttcgaccata caaagttcca atgtatccgt ttattccact agtagcaatc
    21481 attggaggga cttttatcgt atcaagcacg cttatcactc agaccttttt agcttcgatg
    21541 gggatagtat tgacacttgc tggtatcccg atttatctct atttaaaacg tagatagcta
    21601 aaacattcga attttattag aactgcactt ctctgccaaa caaaatattt tatttaaatg
    21661 aggagtacgt ggaagaaatg tcaaaagtac tcgttaattt tgatcacgta aatgtcgttt
    21721 tgtctacgat gaaatagcaa agtaaaagac agaaatcggg ctcaaccgat ttctgtcttt
    21781 tactttgatt tgcgtaactg aactattcgg cgtacgccgc gataaatatt taataaaacc
    21841 aagcaaccag aaatgaaaat gggaagccaa atcagataga cagggtagcc aagcacttta
    21901 gacaaagcat gtggagtcat tttatccatt tcaagaagtg cttgctgcca agtctgaaga
    21961 ttgaagacta gcatgaaacc cgaaataatc aggagaataa atcctaaata atgaattgta
    22021 gttctttttc ttacataagc gatcgtagca taaaggaaca gtaaaaaaat aggtacgcca
    22081 aaaacaaagt acatattcag catctcgaca ccccctttgt gtttatctta ctttactttg
    22141 aaagaaatga gaaataatag cctcgttact tgcttgaaac agcaaaggac tgctaagata
    22201 agagagatac aaagaatcaa ggaggagtca tatggcaagt tatatcgaac cgattcgttt
    22261 tgcgctgatt gtttttccat ttttagcctt agcgatctca tctgtttttt ttatttatga
    22321 atatcgaaag tatggaacat ttctagtaac aagggcagtt attttgtatt ctttcgtttt
    22381 ttatttattg tgtgcgtatt ttttggtgat cttaccactt ccggcaaagg agacggttgc
    22441 ccaaatgaca gggccaaaga tggagctgca tttatttgct tcatggcaac atttcagaga
    22501 caacacggta ttggtcttgt ctgaacctag tacctacctg ccggcaatga agcagagtgt
    22561 atttttggaa ccagctttta atgttttatt actcatacca tttggcattt atttaagata
    22621 ttatttccgg ctttctttta tgaaaacgat gattgctagt ttctgccttt ctctattttt
    22681 tgaattgacg cagctaagtg gactttattt tatctaccca agaccatacc gcttatttga
    22741 tgtgaatgat ttagtgacaa atacattggg tggaatcatt ggttttgtcg ttacgccgtt
    22801 atttacattt atgcttccta caagaagacg aatggatgaa ctttcttatg aaaaaggaaa
    22861 gactgtctca ttgactcgac gcttggtcgc ttatttgatc gactggacat ttttatctct
    22921 tattaatttg gtagcggcga ttttctttaa aattgcgttc gattcagggg cagttacttc
    22981 tcatatctgg tggcttttat tagaagtagg ggtatacttt atgattgtac cgtttataac
    23041 caatggatgg acacttggca aaaaaattgt gcgtatccgt ttggtggaag aagaccgcga
    23101 acgaatttct ttaaaagcat tgatgatccg ctacgggtat ctgtactttc ttttttatgg
    23161 tttaagctgg ttggggcagt tgcttcagca aatcatgaat tcgacaacga atacggtgtt
    23221 gcagatgagt gcagtattca tttctctcgg tgtcttgtta ctacaaggta tttttatttt
    23281 aaatatcttg ctatcatttt tgagaaaaaa acggattctg ttttatgaaa aagctagtca
    23341 cacatatact gttagcacga ttggccaaac aaaggatgat gattctcgaa attagctgag
    23401 caaaaacgct ttgtctgtgt tataatgcca aagtcgtgat aatttaaaga gaagaggtgc
    23461 acgaatgaat aattttgata tattccgctt caaaccgttt atcaatgaag cgttaaccgc
    23521 aaagggcttc aaacagccca cagaggtcca ggaacgtttg attcctttaa ttgcaaaagg
    23581 aaaaaatatc attgggcaat ctcaaactgg tacaggaaaa acacatacgt ttttgttgcc
    23641 tttgtttgaa aaaatcaatg cagaaaaaga ggaagtacaa gttgttatta cagctccaag
    23701 tagggaacta gctgcacaga tctatgaagc agctacacaa atcgctaaat tttctacacc
    23761 ggagatccgt gtctcgaatt ttgtcggcgg tacagacaaa cagcgtcaat taaatcgtct
    23821 gcatcaccaa cagccacagg ttgtagtagg cacgcctggt agaatattag acatgatgaa
    23881 tgaacaagcg ctgaagattc acaccgcatt tgcatttgtt gtcgatgaag cggatatgac
    23941 attggatatg ggttttttaa atgaagtaga ccaaattgca agtcgattac cagaaaagct
    24001 gcagttttta gttttttctg ccactattcc agaaaaactt cgtccattct tacgaaaata
    24061 catggaaaat ccaatcattg aagaaatcaa accaaaagca gttatttctg aagacattga
    24121 aaattggctg atttcaacaa aaggtaaaga tgagaatcaa atgatttatc aactgctgac
    24181 aattggtcat ccttatttag caatcgtatt tgccaataca aaacagcatg tggatgaaat
    24241 tgccgattac ttgaaaaacc aaggcttgaa agtagcaaag attcatggag acatcacccc
    24301 aagagagaga aaacgagtga tgcgacaagt acagaatctg gagtatcaat atgttgtagc
    24361 gactgattta gctgctagag ggatcgatat cgaaggtgtc tctcatgtta ttaatgcaga
    24421 aattccagaa gatctagact tctttatcca tcgtgtcgga cgtactggac gtaatggctt
    24481 aaaaggaacg gccattacgt tatattcgcc aaatgatgag caagccattg atgcgataga
    24541 aaaattgggc gtctcatttg aaccaaaaga aatcaaaaac ggggaaatcg tcaaaacata
    24601 cgatcgtaat agacgtgcca aacgagaaaa atcaaaagat gtattagatc ccacgctgat
    24661 tggtcttgtg aagaagaaaa agaaaaagat caagcctggt tataagaaaa aaatcgattg
    24721 ggcaattgca gaaaaaaata aaaaagaacg caaaatcgaa cgcagacagc aaagccgtac
    24781 tgctcgtaaa aataagaaaa attcacataa ataaaaaaca agaggttgtg acaaaaggcg
    24841 tgtcacaatc tcttgttttt aagctagact ttttttcatt ccgatacaaa gtacttcatc
    24901 ttcaataaaa ggttctgtgc aagtatgata cccttgtttt tcataaaaat ctacagccgt
    24961 catttcagct actacataac tagtctgaca accttctgaa accgcccgtt tttcagcagc
    25021 ttcaagcaac tgaccgccta acccttttcc tcggtattct tttgctacgc aaaatcgttc
    25081 cggcttgaag caagtaggtg ttttcttttc ataacgcaaa gtagctactg gtcttttttg
    25141 atcaagaaat agataatagg tttgttgcgg gatatctttt ccgtcgaatt ctagttctgc
    25201 aggaatccct tgttctaata caaaaacttg atatctcaaa aagcagctag ctgcgcgtat
    25261 ccattcatcc gctccaacta tttttttcat catattcctc cttgtcatga atagcagaga
    25321 tgtattgatt cctttattct tatcgtacca aaaatcgtgg agtatgacca aaaggaaatg
    25381 aaaataaaaa ttgagatatt gacagtgatc aatagaatgc gtatactaat taagtacgaa
    25441 aaggatatga tttgtttttt aagactcaca gaaaattctt tcttggctga aaaaagaata
    25501 gtcactaatc aaattgctcc ctttttctat tttggtagaa ataccgacgc agctcagcgt
    25561 tatcgagttt aagaggtaat gattacacat cattgcaatc aaggtggtac cgcgagtttt
    25621 cgtccttggc tgtgatggtg tttttttgta tttaatttat agaaagcagg taatgacatg
    25681 aaacaattaa gtagtgcaca agttcgacaa atgtttcttg atttcttcca atctaaagga
    25741 catacgattg aacctagtgc gtctttgatc cccgtgaacg atccaacctt attgtggatc
    25801 aactctggtg ttgcaacatt gaaaaaatat tttgatggtt cagttgtgcc agaaaatcca
    25861 agaatcacaa atgcccaaaa atctattcga acaaatgata tcgaaaatgt cggtaaaaca
    25921 gcacggcacc atacgatgtt tgagatgctg ggtaattttt cgattggtga ctatttcaaa
    25981 aatgaagcca tccactgggc atgggaattt ttgactgatc caaaatggat ggcattcgat
    26041 ccagaaaaac tttatgtaac agtttatcca aaagatacag aagcaaaacg catctggcat
    26101 gaagaagttg gcctgccact agatcacatt attgatgtag aagacaactt ctgggatatc
    26161 ggtgcaggcc catgtggtcc ggatacggaa attttctatg accgtggaga agcttataac
    26221 gatttagcag aagacgatcc tgaaaactat ccaggaggag aaaatgaacg ttatttagaa
    26281 atctggaact tagtattttc agaattcaac cataagcctg ataacacatt tgaaccactt
    26341 cctcataaaa acattgatac ggggatggga ttggaacgca tggtctcaat cgttcaagat
    26401 gcgtcaacga actttgagac agacttgttt atgcctatta tccacgccgt agaaaagcta
    26461 agcaaacaag taaaatatgg tgaaaaccca gtaacagata tttcttacaa agtaatcgca
    26521 gatcatattc gcgccctttc atttgctatt ggcgacggtg cattgccatc gaatgaaggg
    26581 agagggtatg tcttacgccg tttgcttcgt cgtgctgtca tgcatgggaa aaaattaggt
    26641 attgaacaag cattcttata tcgtttagtc cctgttgttg gggaaatcat ggtaagttat
    26701 tatcctgaag tacttcaaca aaaagaattt attgaaaaag ttatccgtac agaagaagaa
    26761 cgtttcaatg aaacaatcaa tgaaggtttg gaaatcttaa atcaagtgat ccaagaagta
    26821 aaaaacgaaa acgggacgac tctagcagga aaagacatct tcaaactata cgacacatat
    26881 ggcttcccgg tagaattaac ggaagaggtg gcagaagatg aaggactgaa agttgatcat
    26941 gctggttttg aaaaagaaat ggatgcacaa cgcgaacgtg ctcgctctgc ccgcagtact
    27001 gaaaaatcaa tgggggtaca atctgcttta ttgacagata tcaaagtaga aagtaaattc
    27061 attggataca cagaacaaga atcagatagt caattattgg tcatcgttaa aggtgagtct
    27121 cttttgaatg aagtcgcaga aggtgaagct caaatgatct ttgatcaaac acctttctat
    27181 gcagaaatgg gtgggcaagt agcggatcaa ggatggatca tgaatgaagt aggcgaaatc
    27241 gtagcagttg ttacagatgt tcaaaaagcg cctaatggtc aatttatgca caccgtagaa
    27301 gtaaaagcac caatgagtga aggacaaacc tatcatctat tgatcgatca atcgatgcgc
    27361 aatcggatca tcaaaaacca tacagcaacc catctactgc acaaggcatt gaaagaagta
    27421 cttggcgagc atgcaaatca agctggttct ttagtcgctc ctggacattt acgattcgac
    27481 tttacccact ttggtcaaat cacttcagat gaattagctg aaatggaaag aatcgtcaac
    27541 gaaaaaatct gggaagctat cccagttgaa acaattgaaa cagatatcga tacagccaaa
    27601 aacatgggtg caatggcttt gtttggtgaa aaatacggac atgacgttcg agtagtaaat
    27661 attggcggct ggtcaatcga actgtgcgga ggcacacatg tagcaaatac agaaaacatc
    27721 ggaatcttca aaatcgtttc tgaatcagga attggtgcag gcgtgcgacg aatcgaggct
    27781 gtaacaagta aagaagcgta cgaactactt gaagaagaag aaaaacaact aaaacaagta
    27841 gcaacaattg ttaaatcacc tcaattgaaa gaagttgttt caaaagcgga acaattgcaa
    27901 cagcaagtaa gagagctaca aaaggaaaat gaacaattag ctagcaagtt agcgaatcaa
    27961 caagctggtg atgtattcaa agatccagaa acgatcaatg gaaaaacaat cattgcagca
    28021 caagtaaaag tgaaagatat gaatcagctg cgtcaattag ctgaccaatg gaaacaaaaa
    28081 aatatttcag atattcttgt tttagcaaac gaacaagaag gaaaagtcag tctgctagcg
    28141 gcaatgacga aagaatcaaa tgaagctgga ctaaaagctg gcgacttgat taaagcaatc
    28201 gcaccgaaag taggcggtgg cggtggtgga cgtccagaca tggcacaagc tggcgggaaa
    28261 aatcctgctg gtattccaga tgcacttaaa gctgcgaaag aatgggttgc gcaacaatag
    28321 ggcgtttacg tttgacagaa aaacaaaagt tcaaacattg aacgaaacat gattatgggg
    28381 gcgaagacaa acaagtcttg ccctcatact tttttcaatt ggatggcgca tctaatcaaa
    28441 tggtcggatt cggatggtct ttagttgacg aaagtgacag aaatttttac gattagtata
    28501 aataaaaagg ggtgaaaaaa tgaatagtag aaataagacc tttcgactag tcttacgtgc
    28561 gatcttgtta gcaatcatca tcgtacaagc gatggttcct tggctggggt ttattccatt
    28621 aggattcatt agtttaacaa ttatccatat cacagtaatt attgctgcag tagtattagg
    28681 cccaaaagat ggcatggtga ttggcttgtt ttggggaatc gctacaattg ttcgtgccta
    28741 tgccatgcca acaacgccat ttgatacatt agtgttcacg aatccgatta tctcggtagt
    28801 tcccagagta cttgttggct tagtagcagg cttggttttc cactggattt atcaaaaaaa
    28861 taaatcaata acgatcagct cagcctttgc aggggtactg ggttcattgg tcaatacagt
    28921 cttagtgtta ggattcatgg gattatttta tacaggtgca acggcagatg cctatggtgt
    28981 agactctgct cttttattta aaaccttagc aggggttgca gcaatcaatg ggataccaga
    29041 agcaataggt gcgggaatca taacaccatt actagcaaaa gcgttatttg cagcaacacc
    29101 gttgaaacca gaataatgac catcttttaa tagtaatagg acgcacgata gatagaaaaa
    29161 ggaagagagg agaggaaaac aatcgtcctc tccatctttt tacctgagca acaagcaaac
    29221 tagaaggggt aaaaaacttg gctaagaaac aagaagaaaa aaagaaacaa ataccgcaac
    29281 agcggacaaa aaaaggagta ctagcgggta tcttaggatt ggttacgaat ctgatcttgt
    29341 ttgtctctaa gctaatgatc gggctttttt caggaagtgt ctccattatg gctgatgcag
    29401 tgaacagtct atcagatact gcttcatcca tattgacttt gatcggattt aaaattgctg
    29461 caaagccagc agatcaggaa catccttatg gacacgaacg cttcgaatat atcagtgggt
    29521 tatttgtttc gatcatcatt acttatgtag gatttcaatt tttagagtct tctgctgaaa
    29581 aaatcatcca tccagaaaat gtctctttaa cacccatcgt cttttttgtc ctaatcattt
    29641 ccatcttttt gaaatttgca caaggcaaaa tgtatcaggt tattgcgcaa tccattgatt
    29701 ctgaaacatt gagagctaca gcaacagata gttataatga tgtctttact acactggctg
    29761 tattgctttc agcagcaatc gaatgggtaa ctggatggcg tattgatgga tatataggtt
    29821 ttgctttagc attatatatc ctttatagtg gattgacgat gcttcgaaat tttgtatacg
    29881 aactattagg aagtcgccca acgccagcag agattgaaga aatggaagag catttaaatg
    29941 cttgcgatga tatacttggc tatcatgatt tattggtgca taactatgga cccaataaac
    30001 gctttgcatc agtccacatc gaagtagatg aatcaatgaa tttgaatgtg gctcataaga
    30061 ttattgatct gatagaaaaa gactttaaaa agtctttgga tgtggaactt gtctgtcact
    30121 tggatccaat gcctgttgat aacgaaaaat actataatat cttgcggcag ttaaagcaaa
    30181 ttcttcttga aacaggtaca gggcttaata ttcatgattt tagagtaatt catcaaggaa
    30241 aaacgctgca atttgatgta gtggtccctg agaaattcca gtttgttgat acagaactag
    30301 aaagaatctt acgtagtcaa atagaagaaa aaatcggaga ttaccaattg gaaattacgt
    30361 ttgatcacaa ttatctttta tattaaatga gaaactataa aaagactcct taaagttttt
    30421 aatttaaaga gtcttttttt cttttaattg tttatggaga gcctgacgtg caagattatc
    30481 tgctccttta tttttgtttt cgggcaacca ttgcaggatc aataaagaaa attcggatag
    30541 aagttcttgg attctttgaa ggtagggaag gaagagctcg ttcttcgttg cgtttttgtc
    30601 gaccgtagaa actagtgttt tgctgtctga aaaacagaaa atcgtttcat tctgtaagtc
    30661 gtttttcttt agaaattcta aagtttttaa aaagacttcg aactctgctt gatggtttgt
    30721 ccctatagaa agcgggatag atagctgttc ttgtttacct tgatagacaa tcaagattcc
    30781 acctccacta ggaccagggt tgccttttgt agaggcatca atgtatactt ttaacataaa
    30841 aagagattct cctttttgtt caaactaatg ttatggtaag attatatcat tactgaatcg
    30901 gtgagaggtt agagaatgaa aaaaaatatt cgatggcagc ctgaactggc acatacgatc
    30961 atctattggt cattgacctt gatcattctg ttttttagtc tgattctttc tttagaaaac
    31021 acaggtcctt attggaaaag caattttgtt ttaggtattt ttcttgtttt tctatttcta
    31081 ggtttgcggc gttcttttat tttaaaatca aactctgtga ttatacgtta tgctgctttt
    31141 tggaaagacc gagaaatatc tgtcggacag attcgatctt tgacgccaac taatactggg
    31201 gtgcaaatga tgttgcaaga tgagaaagcc ccatctgttt tcatgatgcg aaaaaaagat
    31261 caagtagtcc tttttgatca ttttgaaaaa aacggaccca aatcagaatc aattccttat
    31321 ccttatacga aaaaaagaaa aagctaattt taaaaatcag aacctaaaaa aacgaatttt
    31381 tacaggaaaa gaaaagagtg agacgtacgg ttcatcccgt ttagcgtctc actctatttg
    31441 tttatctatt catgcatact agtatcggta gtctgaacgt tgcctttctt gacatgggca
    31501 gcttgtgttc ctcgattccc ttcgatgatc tcaaattcga ccacttgatt ttcttccaaa
    31561 gttttgaatc cctcttcttc aatcgcagta aaatgtacaa aaatttcttc tgtgtcatca
    31621 taactgataa agccgtatcc ttttttgttg tcaaaccatt ttacaattcc tgtggtcaaa
    31681 gcactcacga cctttctaaa gtctgatcat agccttcatt ataaagagta tttataagta
    31741 cgtcaaataa attaaaaacc tgtaatttta tgtttttttt ctgaataaaa aaaggtataa
    31801 ttggcatatc ttaacaaaga agatattttg ttgaataaag ttcgtgaaaa ggggtaatca
    31861 aatgaagaat gatctgcaaa tttcacaaga aacagagcta gaaccgattg ttgaagtggc
    31921 tagtaaatta gatcttaagg aagacgattt ggaactgtat gggaaatata aggcgaaaat
    31981 caatttttct ggaatcaatc gatcaaagga taacgctgag gggcatttga tcttagttac
    32041 ttccatcaat ccaacaccag ctggcgaagg aaagtcaacg atcactgtag ggttaggaga
    32101 cgcgctcaat aaaatcaata aaaaatctgt tattgcattg cgagaaccct cacttggtcc
    32161 tgtgatggga atcaaaggtg gtgctgcagg cggaggatat gcacaggtat tgcctatgga
    32221 agatatcaac ttgcatttta ctggtgacat gcatgctatc acaacagcta ataatgcatt
    32281 atctgctctt ttagataatc atattcatca aggaaatgaa ttgaacatcg atgcccgccg
    32341 agtgatttgg aaacgggtag tcgatctcaa tgatcgtgaa ctgcgtaaag taattgttgg
    32401 cttgggtggg ccaatccaag gtgttccaag agaagatggg tttgatatca cagttgccag
    32461 cgaaatcatg gctatcttgt gtcttgcggc agaccttgaa gatttaaagg ctcgtcttgc
    32521 aaggatcgtt gtcggctata catttgaccg tcagcctgtg actgctggag acttgaaagc
    32581 agaaggcgct ctggcattac tgctgaaaga tgcaatcaag ccgaatcttg tacaaacaat
    32641 ttacggaaca ccagcttttg tccacggggg accgtttgcc aatatcgctc atggctgcaa
    32701 tagtatttta gctaccaaga cagcattgag attagcagat tacgttgtca cagaggcagg
    32761 atttggtgca gatcttggcg gagagaaatt tttagatatc aaagtaccta acttgaaaaa
    32821 agcgccggat gctgtggtga ttgtcgccac tgtccgtgca ttgaaaatgc atggcggaat
    32881 gaaaaaagat gaacttaaaa atgagaatct tgatgctttg aaagtaggtt ttgcaaattt
    32941 aaagcgccat atacgtaata tggaacaata tcagctacct gtgatcgtcg caatcaatga
    33001 attcgtaaca gatacagaca atgaactcac actgttggag cacttgtgtg aaaatcaggg
    33061 gattcttgcc aagagagcga gtgtatgggc agatggagca gaaggcggcg ttgatttagc
    33121 aaaagctgta gtacgattga ttgatagaaa agaagctaat tacaagccat tataccgcct
    33181 agaagaaacg atacaagaaa agacagaaac aatcgtcaag aaaatttacg gaggaaatgg
    33241 tgttgttttc agcaaaaaag cgcaaaaaca aatagaagag tttacgaaga atggatggga
    33301 caaactgccg atttgtatgg caaaaacaca gtattcattt tctgatgatc cttcactgct
    33361 aggtgcaccg gaagatttca ccatcacgat ccgcgaaatc atcccaaaat taggggcagg
    33421 atttttagta gccttgactg gagatgttat gacgatgcct ggtttgccta aaaaacctgc
    33481 tgcattgaat atggacgtga caaatgacgg agaagtgttg ggattgtttt aagcttgatt
    33541 ataatctacc atcgaaggag ctgtattttg actacagctt ctttttttag acgataggga
    33601 attaattttg tttacactag gatcattctt tttctttatt gtattttgag ctatactaaa
    33661 gcataatagg agggtatgat gaaaacgaca tttataagaa aaaccggttt ttacggtatg
    33721 ggtagctcga ttcagctaag aatcaacaat gaaaaacgca gtgttctcag tcatgaacaa
    33781 aagataacgc ttgatctgga agtgcccttt acgatgcagg tcacctttta ttggctaaaa
    33841 agccccgttt atacagtgaa agaagcaaag cctgcttatg tgatcacaat gaattacttg
    33901 attcttcaaa tctatccgtt tttatttcta gcaactgggt tgggggccgt tttcctacaa
    33961 aatttatttt tcagtttttt tgcagttatt gtgttgattg cgttcttttt ctatataaaa
    34021 aaccgggcat atttgatcaa ggaggaaaat tatggcaaac tctgatcttt ttttaatcag
    34081 tttcaatcga atcgaaaaat ggatgagaga cgagatggga aatgcacgaa atatgggatt
    34141 taccgaactt gtcagacgcc tagctcaaag aaaacaatta atgatcagaa aatacgaaga
    34201 tgatctttta cagcttgctc agttaaggaa tgcgatcgtc catgatcgga tcgcagtcga
    34261 ttttgtcatt gcagagccaa atgattggac agtcaagcga atccagctga tcgaacaaga
    34321 actgattcag cctgaaaccg tgctgcctcg atttgcaaaa cgtgtaacag gttttgaaca
    34381 agatcttccg ttacttgaac tgttaaagat agtagcagaa aaaaggtatt cacaatttcc
    34441 gttatacaat aaaggaaaat ttgtagcatt gattacttta cgtaatatcg ggttttggct
    34501 agcaaaagaa agccaaaaag gtccggttga tttaacaaat aaacgagcgt tggatttgat
    34561 catccaaaac ggaaagtaca caaattatca ttttgttcca gctaaaacac atatttacga
    34621 ggttgaagcg atgtttcgtg agcaagggac tttggaagcc gtacttatca ctaaagatgg
    34681 gaatcccgat ggtaatctgc tcgggatcgt acgacctcgt gatatttata aacaaataga
    34741 aaaggattga ttttttgtta attatttatt ggatattcag cgctttgcta gtaggagtgg
    34801 atcagttggt caaatggtgg atcactgata atctagcgct tggagagacg aaaaatctta
    34861 taccaggtat tttgtcactg aatcatattc gaaacactgg agccgcatgg agtatgctag
    34921 aagggaagat gtggttcttt actatagtga cactcattgc agtcgttgtg attctcacat
    34981 tgatgattaa aaatcgtgaa aaaggtaatc gctggttcat gattggattg agtttgattc
    35041 tagctggtgc gattggcaat tttatcgacc gtattcgtct tggctatgta gtagatatgt
    35101 tccagacaga ttttatgagc tttccgattt ttaacgtagc agatgtgaca cttgtatgtg
    35161 gagtgatttg tatgttaatt tatattattt tagatgaaaa ggatcaaaga aaaaaatgac
    35221 agaacttaaa acgacgattc accatgaatc tggtaggatc gataaagtat tgactaattt
    35281 attcaccgat tacagtcgtt cgcaaattca gctgtggctg aaaaatggag cggtttctgt
    35341 caatggacag ccagtcaaag caaattacaa agtaaaacaa ggagacgaga ttactgtctt
    35401 gatccccgaa ccagaaacat tagatgtggt cccagaaaat atcccgttga cgatcgttta
    35461 cgaagatgaa gatgtagcgg tgataaacaa acctcaagga atggtcgttc atccttcagc
    35521 aggtcatacg catggcacaa tggtcaatgc gctgatgtac catatcaagg atctttctac
    35581 gatcaacggc gttattcgtc caggtattgt tcaccgaatc gacaaagata cttccggact
    35641 gctaatggtt gccaaaaatg accaggcaca cgaatcatta gctcagcaat taaaagataa
    35701 gacctctttg cgcaaatatg ttgctttagt ccatggagag attcctcacg aaaaaggaac
    35761 catcaatgcg ccaatcggcc gttcgaagga aaaccggaaa atgcaggcga taagagaaga
    35821 tgggaagtcg gctgtcacac attttactgt attggaacgt ttcgaaggat ttactctggt
    35881 tgaattaaaa ctagaaaccg gaagaacaca ccagatccgt gtgcatatga agtatatcgg
    35941 ttatccgtta gctggagatc cagtatacgg accgaagaaa actttgaaag gcaatggtca
    36001 gtttctccat gcgcaaatgt taggttttac gcacccgact acaaacgaaa agatgatctt
    36061 tgaagcacct cttccagaag tttttgaaaa aactttagaa aaattaagaa aaaacattgc
    36121 attttaattt agataacggt atagtaatta acaacgaaag cataaaactt aatagaattc
    36181 ctttaagttt agtcccgtga ggctaagaag ggtgggagca aggatacgta tggtgcgcca
    36241 taagtagatt gctcattgac atagtatagc tatcagtgta tcagtatacg ctcagtctaa
    36301 acattcctcc tgtcctctta gggcaggagt ttttttatgc aaaaaagtag ggggaatgaa
    36361 aatgcaagca aaagaagtag tagatcaggt aaccatgaag cgagcattga ctcgaattac
    36421 ttatgagatt atcgagagaa accatagtat ccaagatatc gtattggtag gtatcaaaac
    36481 acgaggaatc tacattgcat caagaattgc cgaacgcctg aagcagctag aagatatcga
    36541 tattcctgta ggagaactgg atatcacttt ataccgagat gacaaaaaag aaaatccaga
    36601 agaaccggaa cttcattctt ctgacatccc agtttcctta gaaggaaaag aagttatttt
    36661 gattgatgat gttttgtata caggacgaac gattcgagca gcaatggacg cagtaatgga
    36721 ttttggcaga ccaagaaaaa tttcattagc agtcctggtc gatcgtgggc atcgagaatt
    36781 gccaatccgt gcagattatg ttgggaagaa cattccaaca tctcgggcgg aagagatttt
    36841 agtagagatg caagagcttg atggacaaga tcgcatcatg attttgaaag aggaagatta
    36901 gaggagagag aaacatgtcg gagaaaaaag gatttcataa tgatgacgtg gtgttagata
    36961 tccacgatcg cccaacccca ggacattggg taggattaag tttacaacac ttgttcacga
    37021 tgttcggagc aaccgtgtta gtccccatct tagtaggtat cgatccaggt attgcgctag
    37081 tcagttcagg actaggtaca ttggtgtact tatttactac taaaggaaag atacctgcgt
    37141 acttaggaag cagcttcgct ttcattgcag cgatgcagct tttgatgaaa acagatggtt
    37201 atccagcgat tgcacaagga gcagtcacaa ctggattagt ctatttgatt gttgcgctga
    37261 tcatcaaaaa gatcggttca gcatggctgg ataaaatctt gcctccaatc gtcgtcggtc
    37321 cagtcatcat ggttatcggg ttgggacttg cggcaaatgc agcaacaaat gctatgtata
    37381 atcagggcac gtatgatttc aagtatattg ctgtagcgct gatcacatta ggattaacaa
    37441 ttttcttcaa catgtggcta agaggttttc tcggattgat tccgatttta ctaggtatta
    37501 ttagcggata catcgtagcc ttgctgtttg gaatcgtgga tactaagcca atcgtagacg
    37561 ctgcctggtt tgctgttcct aactttgaga ttccctttgt acaatatgaa ccaaagctat
    37621 atcttggagc aatcacaaca atggcaccca tcgcttttgt gacgatgaca gagcatatcg
    37681 gccatcttat ggtattgaat aaactaacta aacgtaattt ctttgaagaa ccgggtcttc
    37741 ataagacgtt gacaggtgat ggattagcac aaatcgtggc agcctttgtc ggaggtcctc
    37801 ctgtaacaag ttacggtgaa aacattggtg ttctggctat cacacgtgtc catagcgtgt
    37861 ttgtgatcgg tggagctgca gtatttgcag tcattttagg attcgtagga aaattaagcg
    37921 ctttgatttt aagtatccct ggaccagtca tctcaggaat cagttttatc ttatttggag
    37981 taattgccgc aagtggaatg aagattttaa tcgagaacaa aatcgatttt gataaaaaga
    38041 aaaatctgtt gattgcttca gttatcttag tagtaggaat tggcggactc gtctttgaag
    38101 taggcacatt tacattgtcg gcaatggcat tagcaactgt acttggcatc attttaaatc
    38161 ttatcttacc agaaacttca cgaagcgaag aaaactaagg aggaaacatt atgatcatca
    38221 aatcagaacg tatcagctta aagcatttat tgaccgttga ggcacttact gatcaagaag
    38281 ttatgggctt gatccgcaga gggcaggaat tcaagcaagg tgctaattgg actccgcaaa
    38341 agcgccagta ctttgctaca aacctctttt tcgaaaatag tactcgcaca cataaaagct
    38401 tcgatgtagc ggagaaaaaa ctagggctag aagtgattga atttgaagca agcacaagct
    38461 ctgtccaaaa aggggaaaca ctatacgata cagtactgac gatgtcagca ttaggcgtag
    38521 acgtagcggt tatccgtcat ggtgatgaga actattatga cgaactgata caaagtaaaa
    38581 caatccagtg ttcgattatc aatggaggag acggaagcgg acagcatcca acccaatgct
    38641 tgcttgattt gatgaccatt tatgaagaat ttggtcattt tgaaggtctg aaagttgcca
    38701 tcgtaggtga tatcactcac tctcgcgtag ctaaatccaa tatgcagatg ttaaaacgtc
    38761 taggcgcaca agtattcttc tcaggtccta gagaatggta cgacgatgaa ttcgaagtgt
    38821 acggacatta tatgccattg gatgaactac tagatcaagt tgatgtcatg atgatgcttc
    38881 gggtgcaaca cgaacgccat gatggaaaag aaagtttctc aaaagaaggc taccatcagg
    38941 aacatgggtt aacagttgag cgtgcgaaga agttgcaaaa acatgcaatc attatgcacc
    39001 cagcaccagt taaccgtgat gtagaattag ctgattcatt agtggaagga ttacagtcgc
    39061 ggatcgttca gcaaatgagc aatggagtat tcgtcagaat ggcaatctta gaagcagtgc
    39121 taaatggaaa agcgtaatta taggaggatg aaaaaatgaa aacactcatt aaaaacggtc
    39181 agatcaatac acgaaaaaat atgacgacac cagcagaaat ttggatagaa gatgggcgaa
    39241 tcaaagcaat cggtacagga ttctcagaag ccgaatttga tgaagtgttt gatgcaaagg
    39301 gacaattgat cacacctggt ttagtggacg tccatgttca tttgagagag ccaggattta
    39361 catacaagga gacgatcgaa gctgggactc gctcagctgc tcgtggcggt ttcactactg
    39421 tatgcgcgat gccgaattta aatcctgtac ctgatacggc agaaaagctg agacaagtat
    39481 atgacatcat tagaaaagat gcagttgtga aggtattgca gtatgcgcca atcacagaaa
    39541 atctgcgaag tgagaaatta gttgatcaag aagcactgat tgaagaaggt gcttttgctt
    39601 tcacaaatga cggtgtaggt gtccaaacag ctgggacgat gtatctggct atgaaagaag
    39661 ctgcaaaaaa caataaagcc ctagtagccc atacagaaga tgaatcttta ttatttggcg
    39721 gcgttatgca cgctggcaag aaggcagaag aacttggtct tcccgggatc ttaagcgtaa
    39781 ccgagtcttc tcaaattgct agagatctgt tgctagctga agcaactggt gttcattacc
    39841 atgtttgcca tgtttctact aaagaaagtg tccgtgtaat cagagacgca aaaaaagcag
    39901 gaatccacgt aacagcagaa gtttcacccc atcatcttat tttgatcgac gaagatattc
    39961 ccgaagattt cggcttttgg aaaatgaacc cgccgctaag aggacgggag gacagagaag
    40021 ctttgatcga aggcttgctt gacggtacga tcgattgcat cgctacagac catgcccccc
    40081 atggattaga agaaaaaagc caaagtttca tgaaatcacc atttggtatc gtaggaagcg
    40141 aaacagcgtt tcaactgatc tatactcatt ttgtagaaac tggtcgattc acgttggaac
    40201 aagtgatcaa ttggttggca gttaaaccgg ctgagatttt tggtttgaat gcaggaacgc
    40261 taacggttgg tgcccccgct gatgtagcag tttttgatat tacacaaact tgcacgatcg
    40321 ataaagaaga ctttttatca aaaggggaga atacaccttt tatcggctgg aaagtaaaag
    40381 gcgaaacaca aatgactttt gtcaacggaa aacttgtgtg gcagaaggga gaataaaagt
    40441 atggaacgtt tgcttatatt agaagatgga acagttttcg aaggaaaggc ttttggtgca
    40501 gatgctagtg tcttcggaga aatcgttttt acaacaagta tgactggtta tcaagaaact
    40561 atcactgatc aaagttttaa cggtcaaatc attacattta cctatcctat ggtgggtaat
    40621 tatggggtaa atagagatga ttatgaatcg atttctccaa catgcaaagg agtagtagta
    40681 aaagaacatg cacgtgtagc ttcaaactgg cgaaaccaaa tgacgctaga ttcatttttg
    40741 aaacaaaaag gaattcctgg aatatcaggt atcgatacgc gcgcccttac tcgcaaaatc
    40801 cgtgaagtcg gaacgatgaa agctagtata gttgatgctg gagattcctt cgaacatgca
    40861 tttgatcagc tgaaagcaac cgttctagca accaatcaag tgcagcaagt ctcaactaac
    40921 aaaccttatc caagtccagg aacaggtcgg aaagtcattg tcattgactt cggcttgaaa
    40981 catagtattt tacgagaatt gagtaaaaga aactgtaacc tcacagtact tccctataac
    41041 acagatgccg agacgatttt agcgctatca ccagacggag tcatgctgac gaatggacct
    41101 ggtgatccaa aagatgtgcc agaagcaatc gaaatgatca aaaaaatcca aggaaaagta
    41161 ccgatttttg gtatttgtct cggtcaccag ttatttgctt tagccaacgg tgcagatacc
    41221 tataaaatga aattcggaca tcgaggatta aatcacccgg tacgagaagt agcgaccgga
    41281 agaattgatt ttacttctca aaatcatggc tatgcagtcg atgaagccac gattgatcca
    41341 gaaaaactca tcgtaactca tgtagaagta aatgacggaa cagtagaagg agtcaaacac
    41401 agagattatc ccgctttcag tgtgcagttt catccagatg ctgcccctgg tccgcatgat
    41461 gcactacatt tatttgatga atttatggaa atgatggacg caggaaagga gcagtaaaca
    41521 gatgccgaaa cgtacagata tcaagaaaat tatggtgatt ggatctggtc cgatcatcat
    41581 tggtcaagcc gcagaatttg actacgcagg aacacaagcc tgccttgcct taaaagaaga
    41641 aggatatgaa gtggttttag taaattctaa tcctgcgacg atcatgacgg ataaagaagt
    41701 ggcagatcaa gtatatatcg aaccgatcac acttgaattt gtatcaagga ttcttcgaaa
    41761 agagcgtcca gatgctctcc ttccaacttt aggcggacag acaggcctca atatggcaat
    41821 ggagctggca gcatcaggta ttttagaaga attgaacata gaacttctag gaacaaagct
    41881 ttctgcgatc gatcaagcag aagatcgtga tctattcaaa cagttgatga aagaattgaa
    41941 tcagccaatc cccgagtctg aaatcgtcaa tacagtagaa gaagctgtag attttgcaaa
    42001 taaaatcggc tatcctgtca tcgttcgtcc agcctttact ttaggcggaa ctggtggagg
    42061 tatgtgtgaa aatgaagaag aattacgtat cgtatcagaa aatggattaa gtctttcccc
    42121 cgctacccaa tgtttgattg aacgaagtat tgcaggtttt aaagaaattg aatatgaagt
    42181 gatgagagat tcggctgata atgcaatcgt tgtctgcaat atggaaaatt ttgacccggt
    42241 gggtatccat acaggtgatt ctatcgtatt tgcccctagt caaacattgt ctgaccatga
    42301 atatcaaatg cttcgtgatg cttctctttc gattattcga gcacttaaga tcgagggagg
    42361 ctgtaatgtc caactcgctc ttgatccaca tagttttaat tattatgtga ttgaagtcaa
    42421 tccgcgtgtt tctcgttcat ctgcgttagc aagtaaggcg actggctatc cgatcgctaa
    42481 gctagctgct aaaatagcgc ttggattaac attagacgaa atgaaaaatc ctgttacagg
    42541 aacgactttt gcagaatttg aacctgcatt agattatgtt gtagctaaaa ttcctcgctg
    42601 gccgtttgat aagtttgaaa acggagaacg gcttttaggc acgcagatga aagcaactgg
    42661 tgaagtgatg gctatcggtc ggaatatcga agagtcttta ttgaaagctg tgcgttcttt
    42721 agaaatcggt acacaccata tcgaacttcc tgagttggca gacatcacgg acgatgacct
    42781 aatccaaaaa gtggttcgag cacaagatga tcgcctgttt tatgtagctg aagctattag
    42841 aagagggtac accatagaag aattagcgga tttgacaaaa atcgatttat tctttttaga
    42901 taaactgttg catatcgtag aaatcgaaca gcagttgaaa cagcagccgt tggatgacaa
    42961 gatattgaaa acagcgaaac aaaacggttt tacagacaaa aaagtggcgg agctgtggga
    43021 aataaacgaa gaagccgttc gccagatgag aaaagaaaat gatttgattc ctgtatataa
    43081 gatggttgac acttgtgcag cggaattcga atcaaaaaca ccttattttt atagttccta
    43141 tgaatatgaa aatgaaagtt cacgatcaga aaagccctca attctagtac tgggatctgg
    43201 tcctatccgt atcggacaag gggttgaatt tgactatgcg actgtgcatt ctgtcaaagc
    43261 aatccagcag gcaggatacg aagcaatcat catgaacagc aatcctgaaa cagtttcgac
    43321 tgacttttct atttcggata aattatattt tgagccattg accttcgaag atgtgatgaa
    43381 tgtgatcgag ctagaacagc cgataggagt aatcgttcaa tttggtggtc agacagcaat
    43441 caacttagcc gaacctttga ctgctgctgg tgtaaaaatc ttagggacaa cgatcgaaga
    43501 cttggatcgt gcggaaaatc gtgatttatt cgagcaagcg ctgaaagagt tggatattcc
    43561 tcaaccttta ggggatactg ctacaagtaa agaagaagcc gtggttatcg cttcgagaat
    43621 tggttatcca gttcttgttc gtccaagcta tgtattaggc ggacgtgcga tggaaatcgt
    43681 tgaaaaccag cgagatttag aagattacat ggaacatgct gtgaaagcat ctccagaaca
    43741 tccagtacta gtggaccgtt acttgcttgg tggtgaatgc gaagtggatg cgatttgtga
    43801 cggtgaaaca gtactcatcc cagggatcat ggagcatatt gagcgtgctg gtgtccattc
    43861 aggggattca atggctgtgt atccacctca aacattatct gaatcgatca agaaaacgat
    43921 cgaagattac acgatccgct tagcattagg tttgaattgt gtcgggatga tgaatatcca
    43981 gtttgtcatc catgaaaatg aggtttatgt gatcgaagtc aatcctcgag caagtcggac
    44041 tgttccattc ttaagtaagg tgacaggaat tccaatggca caaattgcga cgaaagctat
    44101 tttgggagag aaactaaaga cacttggtta tgaaaacgga ctttatccag aaacaaaaga
    44161 agttcatgtt aaagcacctg tcttttcttt tacgaaattg cagaaagttg atacttactt
    44221 agggcctgaa atgaaatcga ctggagaagt aatgggatct gacagacaat tagacaaagc
    44281 tttgtacaag gcattcgaag cttctggatt acacttgcct gaatatggag ccgtattatt
    44341 tacgattgcg gacgaaacaa aagaagaagc actagaatta gctaaacgtt ttacagaaat
    44401 tggctatagt ctgataggaa cgaaacatac agctgcttat ttcgaaaaaa atggtctggt
    44461 cgttgaaaca gtagcgaaaa tttctgagga agcagcagaa aaaaatgtag tcgacttaat
    44521 aagagatggt aaaacacaag tggttgtcaa tacgattgat aaagatcggg gaaatgcttc
    44581 taaagacggt ttcatcatcc gaagagaagc agttgaacat ggtattccat tgtttacttc
    44641 tttagacaca gcagatgcga tcgttcgagt gatggaatcc cgtgcttttt caactaaccc
    44701 tatttaacgg cgagcacatg ttaagacaaa aagacgaaaa accgttgcag gaggggacag
    44761 atgaaacaag aaatgatgga aatcgttcag caaaaacagc tggcaccaaa gatttttgaa
    44821 atgacattga aaggtgatct agtaaaagaa atgcagcaac caggacagtt cttgcatatt
    44881 ctcgtgccgc gtgcagactt gctattacgc cgaccgatta gcttgaatca tatcgaccac
    44941 gaaacaaata cttgccgtat catttatcgg gtggaaggtg aggggactag agtcttctct
    45001 actctttctg ccggagacca tttagatgtc atgggtcctt taggcaatgg ttttgatttg
    45061 tccaatcttc atacaggaga cgaagtattt ataatcggtg gaggaatcgg tgtccctcct
    45121 ctatatgagt tatcaaaaca gttagtcgaa aaaggaatta agccaacgca ttttttaggt
    45181 tttgccacac atgaagtcgt ttattatgaa aaagaattta atcgtctagg agaaacaaga
    45241 atcgctactg acgatggaac atacggtatg cacggaaatg tcggtaattt attgttagga
    45301 acgaagacgg aacctacagc tgtttttgca tgtggttcaa acgggttatt gaagacagtg
    45361 gaacagctgt tttcttcgca tcaaaatgtc cagttatcat tagaatcccg catggcatgt
    45421 gggatagggg cttgctatgc ttgcgtttgt catacgccgg aagatgaaac aggaacaaaa
    45481 agtgtgaaag tttgtgacga aggaccgatt tttaaaatag gagaggtaat tatatgaatg
    45541 agaatccttt agcggtcaaa cttcctggcc tagaattaaa aaatcctatc atgccggcaa
    45601 gcggctgttt cggttttggc aaagaatacg gaaaatacta tgatttgaat cagcttggat
    45661 cgatcatggt caaggcaacg actccaaatg cccgctttgg caatcccacg ccaagagtgg
    45721 cggaaacacc atctggcatg ttgaatgcaa ttggtttgca aaatcctggt ctagacgttg
    45781 ttatgcataa acttcttcca gaattagagg agtacaatga gttacccatc atcgcaaatg
    45841 ttgctggtgc ttgtgaagaa gactatgtag aagtttgtag caaaattggc gaagcaccaa
    45901 atgtgaaagc aatcgagctg aacatttctt gcccaaatgt caaacatggc ggaattgctt
    45961 ttggaacaga ttctgatgtt gcttttcaat taacacaagc agtaaagaaa gtttcttctg
    46021 ttcctgtcta tgtcaaattg tccccaaatg taacagacat cgttccaatc gctcaggcaa
    46081 tcgaagctgg aggggcagat gggtttacga tgatcaatac tcttcttggt atgcggattg
    46141 accttaagac aagaaaaccg attttagcca atcaaacagg tggactctcc ggaccagcaa
    46201 tcaagcctgt cgctattcgt ttgattcacc aagtagcaag tatatccagc cttccaatca
    46261 ttgggatggg cggcgtgcag acagtggatg atgttcttga gatgtttatg gctggagcaa
    46321 gtgctgtagc tatagggacc gcgaatttca ccgatccgta tatctgtcct aaactaatta
    46381 aagagctccc tgtgcgaatg agcgaactag gcatcgaatc gctggaaaga ttaatcaaag
    46441 aagtgagaga ggaacgtgaa cgatgaatca actagaccaa cgaccaatta ttgcattaga
    46501 tttttcaact aggcaagagg tagaagactt tcttcgattt ttcccgaaag aagaaaaatt
    46561 gtttgtgaaa atcggaatgg aattgttcta ccaagaagga cctgaaatcg tacgttatct
    46621 aaaagaggct ggtcataatg tatttttaga tctgaaactt cacgatattc cgaatacggt
    46681 cgaaaaagca atgcggggat tggcaaagct tggtgttgat ctcacctgtg ttcatgccgc
    46741 tggcggtatt cgtatgatgg aggcggcaat gcgaggatta gaagagggga ctccagaagg
    46801 tggaaaacgt ccgctacttc tagctatcac tcagcttact tctacaagtg aagaagaaat
    46861 gcatgccgat caactaatcg aagtttcttt agaaaaaagt gtgatccatt atgctagctg
    46921 tgccaaaaaa gcaggattag atggtgtggt ttcttctgct tgggaagtag aagcgatcaa
    46981 agaaatggca ggagacgaat ttgtttgctt aactccgggt ataagaccag aaggaacagt
    47041 cgcaggagat cagacaagag tagtcacacc ttctcaggca aaaaagatcg gctcaacttt
    47101 tatcgttata ggtcgaccaa tcactcagtc aactaatcct tataaagcat atcaaacgat
    47161 tcaaacagaa tggagctaac ctaaaatgaa tgttgaacaa agtatcgcaa aagacttatt
    47221 agaaattgaa gcagtatttt taaatccaag taatcctttt acatgggcaa gtggaatcaa
    47281 aagcccaatt tactgtgata accgaatcac gatgagctat ccaaaagtcc gaaaagagat
    47341 cgcaaaagga ttagcaagca aaatcaaaga agcttaccct gaagtacaag tgattgctgg
    47401 aactgcaaca gcaggaatcc ctcatgccgc atgggtagcg gaaatactgg atttgccaat
    47461 ggtctatatt cgaagtaagg cgaaagatca tgggaaaggt aaccaaatcg aaggacgtat
    47521 atttgaaggt cagaagatgg ttgtgatcga ggatttgatt tctacaggcg gaagtgtctt
    47581 ggaagcagct gaagcagcaa aaagagaagg tgcggatatc cttggtgttg cggctatctt
    47641 tacgtatgag ttgccaaaag gaaaagcaaa tttcgaaaaa gccgaaatcc ctcttatgac
    47701 gttgaccaat tattctgtat tgattgaagc agctttagaa gatcgttata tcgatgagca
    47761 agaattaact ctgctaaaag aatggaaaaa agatcctgaa aattggcaga tttaagcgcg
    47821 cttgatgaag atagagaaat ccgatttgtg tctccagaat catcaattgg aatagcaaaa
    47881 atccaaaaac tgattgcgag aagcatcttt tcatcaggta tgaaaagaaa cttctatcgt
    47941 gatcagtttt tgattttttt aaaattcatt tttctaagac tggttagcag aaagaagaga
    48001 ataatctctt ctcttttttc tattatatat agattttctc tattttttgg taagggagct
    48061 gtttcaaaaa tggtaaagag cggaaatgtt gcgaatatca gttatttaat gatatgatat
    48121 tcggaaaaag aaatctcaaa gggaggtaac ttttatgggg ttgaatgaat ttattttagg
    48181 attgaacaat ttagagacga tcacaagggc acctggattt tttaaatata cagagcatac
    48241 agttgcagca cattcgtatc gtgtagcatc tattgcgcaa gttctaggag acattgaaga
    48301 agcgaatgga caaccgatcg attggaaact gctctatgaa aaatcattga atcatgacta
    48361 cacagagcgt tttattggtg atatcaaaac gccggtgaaa tacgcaagca aagagttaag
    48421 agggatgctg caaacagttg aagaaaagat gacggacgaa ttcatcaacc aagagattcc
    48481 agctgagttc caagaagttt atcgccgtcg gttgttcgaa ggaaaagatg aaaccgtaga
    48541 aggagaacta ttagccattt cagataaggt tgaccttctt tatgaatctt ttgatgaaat
    48601 catcaaaaat aacccggaaa aagtttataa ggagatgttc attgaggcag tgactacgat
    48661 taaaagcttt gaacatcggc cttctgttca gtatttcttt aaagagatat ttccggaact
    48721 attgaaccag gaattttatg gaaaagaggc gtttttagaa accgtctcaa cgattgtaaa
    48781 gaaagaagaa aaataaaaat gatatgagat gcagggggaa gtatagttga aaaagaatat
    48841 ggatgtcgaa tggggctatt ctggagacaa aggtccggaa aattgggcga gtttatgtga
    48901 ttggtttgcg agaggtgcgg aatttccatt gcagtcgcct attcatttga ccaaggggga
    48961 agaaaccaaa atagaaggag acacgctttc ctttcattat caaaagcaac ggtttactga
    49021 caaggaattc aaaaacacga ttcatttagt accttttgac caactcagtt atgtagagtt
    49081 caagaaagaa cgctattatt tgacagatat tcatttccac ttgccttctg aacatatcat
    49141 caacggaaaa caggaagata ttgaatttca ttttgttcat atgaacagca aaaaagagaa
    49201 tctagttgtg ggtgtcatgt accagctaat ggtacaggaa ggttggctgt ataatcaaga
    49261 aaatggggaa agttggaact tagaaaaaca tgagcattgg gtaaatccgg atgtgttctt
    49321 tccggagcaa aaaagccatt tccattatat tggctcattg acaactccgc ctaccagcgg
    49381 gccaatccaa tggtttgtaa tggatcatgt aggaaaactg aaccaagagt ttcttctccc
    49441 cttcgatggt cagtatgctc gaccgaacaa ccgaccaatc cagcccttga atggaagaga
    49501 aatttattat ttcgaggagt ttgagcaatg aatattcagc agatgaaata cgtggtcgct
    49561 gtagcaaaca atggtagttt cagagaagcg gcaaagaaaa tgtttgtctc ccagccaagt
    49621 ctttcagctg gtatcaaaga attagaaacc gagttaggga tcacgctgtt tactcgaacg
    49681 aatcgagggg catatttaac agaagaaggg caagacttct taaatcgtgc cgagaaaata
    49741 ttggtccagc ttgatacatt agaaactcac tatctaacga gtgaaaagag agacaatttt
    49801 tcgatcgctg cccagcatta tgatttctta ggtcctttga ccgcacagct actcgaggaa
    49861 ttcaaagacg agatcaaaca atttcggatc atggagacga caacagcgag agtgatcgaa
    49921 gaagtcaaag agcagcacag tgagatcggc attatttata tgaatgagca taatcaagct
    49981 ggtatcagaa gatatttaca acaaggagag ctaagtggcc gcagcattgg aaaattcaaa
    50041 acgcatattt ttttaaggca aggacatcca ttagctgaac acgcagagat caccaaagaa
    50101 gaattgttat cttatcctca agttcgtttt acacaggatg gaaataattt cccgtatttt
    50161 tatgaagacc ttatcgagat ccctgatcaa gaaacagtga tttatacgag tgatcgaggt
    50221 actttgatga atatcgttct tgaaactgat gcttacgctt caggatctgg tattgtgatt
    50281 ggagggattc gcgagcactt gcggttaatc cctttggcag aaggcgaact aaatgaattt
    50341 tatatcattt attcggcgaa acgtccgctc agtgaaatcg ctcaaagatt tatcaaagag
    50401 cttactcggt tactggacca gcagagctaa gaaaagcaga tgagaacgct gtttattgaa
    50461 caccgttgat caaaaaagag gggttgtgac agaattttgt cacaactcct ctttttgtag
    50521 caaacaattt ataactaatt gctgttgtgg gaaagtacgc aaatatgatt ctagaaacac
    50581 tcattgatta cctgttttgt ttcatagctg taattttttc ttcttctggt gtcacgataa
    50641 tcgtcttttg gttttcataa ataacataac caggcttggc gccatttggt tttcggatat
    50701 gctttacctg tacaagatca accggcactt gggcagagta tcggtatttt gaaaaatagg
    50761 cagctaactc ggcagcttct gtaattgttt catctgaggg cttgtcactt ttaatgatga
    50821 cgtgcgaacc gggtatattt tttgcatgaa gccaatagtc ggttttttta gctgtcttca
    50881 ttgtcagctg gtcattttga agattgtttt taccaacaag aatgagcgtt ccgtcagaag
    50941 aaaagtattg atcaggctga gatggtttct tacgtttttg tttttttaag ttcttctttt
    51001 tcaggtatcc ttctgaagtc aattcttctt taattgcttc gatatccatt gggccggcaa
    51061 tttccagttg agataagaca gattcaagat attggatttc atctttggct tcttggattt
    51121 gttcgccaat cagcttaaca gcgttcttca gcttttggta tcgatggaaa tatttctgtg
    51181 cattttgatt tggtgtcaat gccggatcta gtgctatttt gattggccga tcttcttcat
    51241 aataattagg taaaacaact tcttttgcgc cgcgtggtac ttgtgtcaga aaagtagtaa
    51301 gtagttcgcc atttctacgg taattctcag cgttttcgga ttctttgagt gtttgttcac
    51361 gttttttcaa tttattttta tttcgtttta gttcattttc gatttttcga atcagttctc
    51421 cgccttgttg ttttgcccga tctttctctg ctttttcgtg ataatagcta tctaataatt
    51481 gactgagtgt tggataagct gttacagcag atgcctgttc ggacaatacc tgataagcaa
    51541 tcggtgtgaa aaaatcttta ttttccgttt cgtaaaaagt aggaataggc tgatggctga
    51601 tggctgagaa aaattcgtcc catacaacca tcttttcatt aggacgttcc agtaatcttt
    51661 tggtcagctc ttgagcgata tcgaaaccga ttccctgaaa ctgacgttgg attgcttttg
    51721 gatctagttc agtttgggaa agccgctgaa agattttttc tttctcggag ctaaatgggt
    51781 tcaagacctc ttgttttggc ggagcaacat attctacacc aggaagcaaa gaacgataag
    51841 tgttttgaga gcttccaatg tgtttgatcg catccagtat ttttccagtt tctcgattaa
    51901 ctaagacgat cgtactatga cgtcccatta attcaacgat caatatgata ttttgtagat
    51961 cacccaactc gtttcgttta gcaaagtgga aatggatcac tcgatcattt tcgatttgtt
    52021 cgatgtcttc taaaatagcg ccatccaaat attttcgaag catcatcacg aagttaggcg
    52081 gattatcagg attctgataa tcgatttggg taatctgtac acgagcataa cttgggtgcg
    52141 cagataataa taatcgatgg tttttgccgc gtgaacgaat caccagcacg atttcatttt
    52201 cataaggctg atggattttc gagatccggc cagatagtaa cgtttcacgc aattcgttga
    52261 ccatcgcgtg ggtaaataca ccatcaaagg acatggttca ttcctcattt cttttcataa
    52321 gtatattcat tataacgaaa aaaagaagaa aaaccaattt ggccgaatag taaagcaaaa
    52381 gtctagaaaa atcttggact aattacataa aaaacagtgt ttctatcgtt gaaatccaaa
    52441 tttttaggt
//
LOCUS       DACIFS010000013        51404 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.013, whole genome shotgun sequence.
ACCESSION   DACIFS010000013 DACIFS010000000
VERSION     DACIFS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.013"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(61..2424)
                     /locus_tag="IYA83_000762"
     CDS             complement(61..2424)
                     /locus_tag="IYA83_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ8586214.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="IYA83_000763"
     CDS             2872..3093
                     /locus_tag="IYA83_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8586215.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IYA83_000764"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="IYA83_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ8586216.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IYA83_000765"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="IYA83_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ8586217.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="IYA83_000766"
     CDS             complement(5894..6205)
                     /locus_tag="IYA83_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ8586218.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="IYA83_000767"
     CDS             complement(6202..6495)
                     /locus_tag="IYA83_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ8586219.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IYA83_000768"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="IYA83_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ8586220.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IYA83_000769"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="IYA83_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ8586221.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="IYA83_000770"
     CDS             complement(8333..12685)
                     /locus_tag="IYA83_000770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ8586222.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="IYA83_000771"
     CDS             complement(12897..14606)
                     /locus_tag="IYA83_000771"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ8586223.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IYA83_000772"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="IYA83_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ8586224.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="IYA83_000773"
     CDS             complement(16103..16903)
                     /locus_tag="IYA83_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ8586225.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="IYA83_000774"
     CDS             complement(16900..17712)
                     /locus_tag="IYA83_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ8586226.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IYA83_000775"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="IYA83_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ8586227.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="IYA83_000776"
     CDS             complement(18467..19189)
                     /locus_tag="IYA83_000776"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ8586228.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="IYA83_000777"
     CDS             complement(19325..20206)
                     /locus_tag="IYA83_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ8586229.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IYA83_000778"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="IYA83_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ8586230.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="IYA83_000779"
     CDS             21446..21925
                     /locus_tag="IYA83_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ8586231.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="IYA83_000780"
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="IYA83_000780"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ8586232.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23226..24011
                     /locus_tag="IYA83_000781"
     CDS             23226..24011
                     /locus_tag="IYA83_000781"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ8586233.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="IYA83_000782"
     CDS             complement(24357..26048)
                     /locus_tag="IYA83_000782"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ8586234.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IYA83_000783"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="IYA83_000783"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ8586235.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IYA83_000784"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="IYA83_000784"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ8586236.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IYA83_000785"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="IYA83_000785"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ8586237.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="IYA83_000786"
     CDS             30332..31033
                     /locus_tag="IYA83_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ8586238.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IYA83_000787"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="IYA83_000787"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ8586239.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IYA83_000788"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="IYA83_000788"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ8586240.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IYA83_000789"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="IYA83_000789"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ8586241.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IYA83_000790"
     CDS             complement(33615..34439)
                     /gene="tsaB"
                     /locus_tag="IYA83_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ8586242.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="IYA83_000791"
     CDS             complement(34528..35508)
                     /locus_tag="IYA83_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ8586243.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IYA83_000792"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="IYA83_000792"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ8586244.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IYA83_000793"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="IYA83_000793"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ8586245.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="IYA83_000794"
     CDS             38266..38433
                     /locus_tag="IYA83_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ8586246.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="IYA83_000795"
     CDS             complement(38495..40306)
                     /locus_tag="IYA83_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ8586247.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="IYA83_000796"
     CDS             complement(40303..40668)
                     /locus_tag="IYA83_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ8586248.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="IYA83_000797"
     CDS             complement(40831..41226)
                     /locus_tag="IYA83_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ8586249.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="IYA83_000798"
     CDS             complement(41244..42206)
                     /locus_tag="IYA83_000798"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ8586250.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="IYA83_000799"
     CDS             complement(42206..42418)
                     /locus_tag="IYA83_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ8586251.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="IYA83_000800"
     CDS             complement(42439..43137)
                     /locus_tag="IYA83_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ8586252.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="IYA83_000801"
     CDS             complement(43157..43699)
                     /locus_tag="IYA83_000801"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ8586253.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="IYA83_000802"
     CDS             complement(43831..44835)
                     /locus_tag="IYA83_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ8586254.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="IYA83_000803"
     CDS             complement(44832..45821)
                     /locus_tag="IYA83_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ8586255.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="IYA83_000804"
     CDS             complement(45818..46624)
                     /locus_tag="IYA83_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586256.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="IYA83_000805"
     CDS             46790..47746
                     /locus_tag="IYA83_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586257.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="IYA83_000806"
     CDS             complement(47823..48341)
                     /locus_tag="IYA83_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ8586258.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IYA83_000807"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="IYA83_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ8586259.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="IYA83_000808"
     CDS             48806..49252
                     /locus_tag="IYA83_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ8586260.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IYA83_000809"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="IYA83_000809"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ8586261.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        14445 a        10986 c         9025 g        16948 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgga acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca taga
//
LOCUS       DACIFS010000014        51186 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.014, whole genome shotgun sequence.
ACCESSION   DACIFS010000014 DACIFS010000000
VERSION     DACIFS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.014"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            120..983
                     /locus_tag="IYA83_000810"
     CDS             120..983
                     /locus_tag="IYA83_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ8586262.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQAIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1112..2734)
                     /locus_tag="IYA83_000811"
     CDS             complement(1112..2734)
                     /locus_tag="IYA83_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8586263.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2983..3504
                     /locus_tag="IYA83_000812"
     CDS             2983..3504
                     /locus_tag="IYA83_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586264.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="IYA83_000813"
     CDS             complement(3690..5420)
                     /gene="spxB"
                     /locus_tag="IYA83_000813"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ8586265.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5585..6403
                     /locus_tag="IYA83_000814"
     CDS             5585..6403
                     /locus_tag="IYA83_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ8586266.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6506..6703)
                     /locus_tag="IYA83_000815"
     CDS             complement(6506..6703)
                     /locus_tag="IYA83_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586267.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6872..7450
                     /locus_tag="IYA83_000816"
     CDS             6872..7450
                     /locus_tag="IYA83_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ8586268.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8496..8999)
                     /locus_tag="IYA83_000817"
     CDS             complement(8496..8999)
                     /locus_tag="IYA83_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ8586269.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9358..10383
                     /locus_tag="IYA83_000818"
     CDS             9358..10383
                     /locus_tag="IYA83_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ8586270.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10730..11608
                     /locus_tag="IYA83_000819"
     CDS             10730..11608
                     /locus_tag="IYA83_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586271.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12084..12200
                     /locus_tag="IYA83_000820"
     CDS             12084..12200
                     /locus_tag="IYA83_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ8586272.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13128..13466
                     /locus_tag="IYA83_000821"
     CDS             13128..13466
                     /locus_tag="IYA83_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586273.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13560..13790
                     /locus_tag="IYA83_000822"
     CDS             13560..13790
                     /locus_tag="IYA83_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586274.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14190..14561
                     /locus_tag="IYA83_000823"
     CDS             14190..14561
                     /locus_tag="IYA83_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ8586275.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14835..15527)
                     /locus_tag="IYA83_000824"
     CDS             complement(14835..15527)
                     /locus_tag="IYA83_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ8586276.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15530..16315)
                     /locus_tag="IYA83_000825"
     CDS             complement(15530..16315)
                     /locus_tag="IYA83_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8586277.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16552..17952
                     /gene="lacG"
                     /locus_tag="IYA83_000826"
     CDS             16552..17952
                     /gene="lacG"
                     /locus_tag="IYA83_000826"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ8586278.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17977..18294
                     /locus_tag="IYA83_000827"
     CDS             17977..18294
                     /locus_tag="IYA83_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ8586279.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18307..19980
                     /locus_tag="IYA83_000828"
     CDS             18307..19980
                     /locus_tag="IYA83_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8586280.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19994..21625
                     /locus_tag="IYA83_000829"
     CDS             19994..21625
                     /locus_tag="IYA83_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ8586281.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22497..23687
                     /locus_tag="IYA83_000830"
     CDS             22497..23687
                     /locus_tag="IYA83_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ8586282.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYSPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23821..26010
                     /locus_tag="IYA83_000831"
     CDS             23821..26010
                     /locus_tag="IYA83_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8586283.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26007..27800
                     /locus_tag="IYA83_000832"
     CDS             26007..27800
                     /locus_tag="IYA83_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ8586284.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28000..28314
                     /locus_tag="IYA83_000833"
     CDS             28000..28314
                     /locus_tag="IYA83_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ8586285.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28335..28712
                     /locus_tag="IYA83_000834"
     CDS             28335..28712
                     /locus_tag="IYA83_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ8586286.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28722..29495
                     /locus_tag="IYA83_000835"
     CDS             28722..29495
                     /locus_tag="IYA83_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586287.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29517..30920
                     /locus_tag="IYA83_000836"
     CDS             29517..30920
                     /locus_tag="IYA83_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ8586288.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31102..32286
                     /locus_tag="IYA83_000837"
     CDS             31102..32286
                     /locus_tag="IYA83_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ8586289.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32283..32573
                     /locus_tag="IYA83_000838"
     CDS             32283..32573
                     /locus_tag="IYA83_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586290.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32570..32791
                     /locus_tag="IYA83_000839"
     CDS             32570..32791
                     /locus_tag="IYA83_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586291.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32833..33612
                     /locus_tag="IYA83_000840"
     CDS             32833..33612
                     /locus_tag="IYA83_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ8586292.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33679..34173
                     /locus_tag="IYA83_000841"
     CDS             33679..34173
                     /locus_tag="IYA83_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ8586293.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34229..34867
                     /locus_tag="IYA83_000842"
     CDS             34229..34867
                     /locus_tag="IYA83_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586294.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34939..35334
                     /locus_tag="IYA83_000843"
     CDS             34939..35334
                     /locus_tag="IYA83_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8586295.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35318..37771
                     /locus_tag="IYA83_000844"
     CDS             35318..37771
                     /locus_tag="IYA83_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ8586296.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37768..39795
                     /locus_tag="IYA83_000845"
     CDS             37768..39795
                     /locus_tag="IYA83_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586297.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39792..40814
                     /locus_tag="IYA83_000846"
     CDS             39792..40814
                     /locus_tag="IYA83_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ8586298.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40831..41760
                     /locus_tag="IYA83_000847"
     CDS             40831..41760
                     /locus_tag="IYA83_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8586299.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42005..42121
                     /locus_tag="IYA83_000848"
     CDS             42005..42121
                     /locus_tag="IYA83_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ8586300.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            42137..42940
                     /locus_tag="IYA83_000849"
     CDS             42137..42940
                     /locus_tag="IYA83_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ8586301.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            43115..43411
                     /locus_tag="IYA83_000850"
     CDS             43115..43411
                     /locus_tag="IYA83_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586302.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            43418..45370
                     /locus_tag="IYA83_000851"
     CDS             43418..45370
                     /locus_tag="IYA83_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ8586303.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(45442..45939)
                     /gene="dfrG"
                     /locus_tag="IYA83_000852"
     CDS             complement(45442..45939)
                     /gene="dfrG"
                     /locus_tag="IYA83_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ8586304.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            46301..47341
                     /locus_tag="IYA83_000853"
     CDS             46301..47341
                     /locus_tag="IYA83_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ8586305.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            47439..47627
                     /locus_tag="IYA83_000854"
     CDS             47439..47627
                     /locus_tag="IYA83_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8586306.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(47685..48038)
                     /locus_tag="IYA83_000855"
     CDS             complement(47685..48038)
                     /locus_tag="IYA83_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586307.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            48569..48991
                     /locus_tag="IYA83_000856"
     CDS             48569..48991
                     /locus_tag="IYA83_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ8586308.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            48988..49218
                     /locus_tag="IYA83_000857"
     CDS             48988..49218
                     /locus_tag="IYA83_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586309.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            49716..49916
                     /locus_tag="IYA83_000858"
     CDS             49716..49916
                     /locus_tag="IYA83_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ8586310.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            49943..51136
                     /locus_tag="IYA83_000859"
     CDS             49943..51136
                     /locus_tag="IYA83_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8586311.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT        16968 a         8426 c         9972 g        15820 t
ORIGIN      
        1 tcacaacctc ttattttgat tagaacttgc gaaaataaac cggttaaagt gtgtctagcg
       61 caagagattt gctataatag acgataaggg taaaatgtaa cgagaagggg ttgaaaaatg
      121 tgtatcaaat tgtaacagat tcttgttgtg atctgccata ccatgtatta gcaaaagaaa
      181 aagttgattt tttatcaatg aatctccaat tgaatgatca agagttagta gatgacctcg
      241 gaaagacatt caattatgac cgctacatga atcagctgaa aaatggtgcg atgcctacga
      301 catcacaaat caatgttggc cgatatttag aatttttccg ttcttatgcg gaaaaacaga
      361 ttcctgtttt atatgtggct ttttccagtg gattaagcgg ctcttacagt agtgcgcttc
      421 aagcggttga attgctgaag gaagaatatg aagaccctga gatctatgtt tttgatacaa
      481 aagctgcaag tattggggaa gggcttttag tattggaagc tgtaagattg aaaaaagaag
      541 gtaaaagcat cgaagaaacc ctagagtggc tggaaagtaa ttatttgaaa gtccattcat
      601 gggtaacagt agacgatctg aaatatctag aacgaggcgg taggatttcc aaagcaagtg
      661 ctactcttgg cagtctattg agtgtaaagc ctattattac ggtggataaa gaaggaaggc
      721 tgacgaatgt tgacaaagtt cgaggaagaa acaagtcgat ccaggcaatc gtcaatgaga
      781 cggtaaaaac aatcgttgca ccacttgatc aaatcgttta tgtggcttac gctggggatc
      841 tagagagcgc ggagaaggca aaacggcttt tggaagagca gataaagatg aaagatgtga
      901 agctttatcc cttaggacca acgatcgcca gtcacacagg ctatggctgt atcgcgattt
      961 tttcgatggg aatcagtcga taaaagcagt aactcattct gtaaaaaagc ccagaaattg
     1021 attgtaacaa aaacagcgac tcttaaaaga tagatgagca tcctatctct tagaggtcgc
     1081 tgttttttta ttttttaagc taaataggcc attatgccca taactcgttc aattgttttt
     1141 gaatatctgc attttccaaa aattcatcat atgtcgtatc agctcgatcg atcacacctt
     1201 tatccgaaac ggcaatgatg cggtttgcca atgtttggat aaattgatgg tcatgtgacg
     1261 caaataagat tgagccagta aatgccatca agccgtcatt caatgctgtg attgattcta
     1321 agtctaaatg gttcgttgga tcatctaata caagtacgtt agctttcgaa agcatcattt
     1381 ttgaaagcat gcaacggact ttctctccac cagataaaac attcactggt ttcaatactt
     1441 cttcaccaga aaacaacatt cttcctaaga agcttcttaa gaaggtgtta tcatcttctt
     1501 ctttacttgc atattgacgc aaccagtcta aaatcgttag atcagtatcg aattctttcg
     1561 ttgtatcctt tggtaaataa gaattacttg ttgtcacacc ccaacgtact gttccagtat
     1621 ctggcgtaat ttcacccatg ataactttga atagcgttgt tgttgttata tcattttctg
     1681 cgacgaaagc gaccttgtct tctttgctca aattaaaaga tatatcatct aaaattttct
     1741 ttccatcaat cgttacactt acattttcta cctgcaataa gtcgtttcca atctcacgtt
     1801 caggtttgaa tccaacgaat ggataacgtc tagaagaagg ttggatatcg tctaaagtaa
     1861 ttttttcaag cattttttta cgtgaagttg cttgttttga ttttgatgca ttcgcactaa
     1921 agcgggcaat aaattcttgg agttctttga tttgttcttc ttttttggca tttgcgtttg
     1981 cttgaagttt agccgctagt tgactagatt ccaaccaaaa atcataattt cctacataaa
     2041 gtttgatttt accaaaatct agatccgcca tatgtgtaca tactttgttc aaaaagtgac
     2101 ggtcatggga tacaacgatc acagtatttt caaaattgat caaaaattct tccagccaat
     2161 tgatcgattg gatatccaga ccgtttgttg gttcgtctag caaaagaaca tcaggtttac
     2221 caaatagtgt ttgtgctaat aacactttta ccttttgtcc accagttaat tcactcattt
     2281 gctgatggtg caattcttca gggatgttca gaccttgcag taaaacagct gcttctggtt
     2341 cagcttccca tccattaagc tcagcaaatt ctccttctaa ttcagctgct cgaataccat
     2401 cttcgtcagt gaaatcctct ttcatgtaaa tcgcatcttt ttctttcagc acttcataca
     2461 gacgcttgtg tcccatgatg actgtttcaa gtacagtata gtcttcatag tcgtaatggt
     2521 tctgtttcaa tacggccatc cgttcatctg gtcccatagt aacggaacct gtggaaggct
     2581 gcagatcccc tgataacact ttaaggaacg ttgatttacc tgcaccattt gcaccaatca
     2641 agccatagca gtttccgggg gtaaatttaa tattcacatc atcaaatagt ttgcggtctg
     2701 aaaagtgcaa actcacatca tttacagtaa tcaatttttt tcctcacttt tatcattatt
     2761 agttttgaca agtcttatag aattatatag gggaaaggta aaagattcaa ggcaatcgaa
     2821 cattctttgt gtttcacgtg aaacagaatg aaaaaaataa aaataaaagc ggagcgaaaa
     2881 tagctactat aggatttatt agtttgcaac agcataattt ttgacgttat ttataagagg
     2941 gagtactata aaactgtaaa agaaatgaaa gaaggattga aaatggatga aaaaagaacc
     3001 atcgactggg gctcattgat tttgggaatt ttatttgtac ttgtctcatt gttatcattt
     3061 caagaccctg tcggaaattt agttgcaatt gtagtagtct ttgcgatttt tgcctttatt
     3121 aaagggatat ttgaactgtt tgtacggaac agattaaaag agctaacagg atataaagga
     3181 aagatgccct taataatcgg gatcatcgat attctagttg gcgtcttttt cttattcaat
     3241 attggtgcag gagtcgcagc attgcctttt gtttttgcag tatggtttat tgctgattca
     3301 gttttagcgt tatttactgc tgacttggca agaggagtaa gtgaaggata ttattggttt
     3361 acgatcatcg tcaatatttt aggaatttta ttaggaattt tcttattgtt taatccaatt
     3421 tcttctgcgt tgacattaag ctttttagtt ggattctatt ttatgttatt tggtatcaca
     3481 catatcgtct atgcatttag ataagtaaaa tcaaacaaat aaaaaagccg gcttccgatt
     3541 acagttttac tgttgtaatt ggaagccggc ttttttatca tggacaaata tgcacgaggt
     3601 gatttttagg tgtcgagaca aatttatgca aaaatgtgag aagctatata cacaaatttt
     3661 atcttaatac tcgaagcata gcgcagttgt cacaacctct tttcatataa ttcattcaat
     3721 actggcaaat catggacttc atatttttct ttgaatgctt ggatttcttc ttcagaatat
     3781 tttcttggat ctaaatgcag atcttctact ggcaatgggc gcttgttatt gatcttcaca
     3841 tcgattacta caggacgatc tgattttgca gctgcatcaa aggcaaatcg aagttgcgag
     3901 tattctgtga ctgtaaagct atttgccccg agcgcttctc caactttacc gaaatctgca
     3961 tcttctaaaa atacaccaaa tttcttttgt tccgtatctt cttgttctgc ttcgatgaaa
     4021 ccaaaagaat cattcgaaaa gacaacatta ataattggaa gctgatattt gacttgtgta
     4081 atgatatctt gcatgaccat agcaaacccg ccatcaccac ttaaggtaaa tacttgtttt
     4141 tctgggtagc ttagttgtgc ggcaattcct cctggcacac cattacccat agttgcgaac
     4201 catccagatg tcgtatgttt ttgtttaccg ttcatattca ataaacgaat agaatggata
     4261 gtcgtgttac cgacatctgt gacgaaaatc gcatcgtctt cagcaatacg attgatttct
     4321 ttatagacag cctctggacg aatcggttct tcctctcgat cttcaaaaga tttcaaccaa
     4381 tttacccatt tttctttatt ttcttgattt gctaggtacc agctatcagc agggcgagca
     4441 tcccctaact cgacaagtct ttgcaaagct gtgtttgcat caccgaggat cgacaaagat
     4501 gtttcatggc gtcgtccaaa ttttgacgca tcgatatcga tttggataaa ttcagcagtt
     4561 ggattaaaaa atgcacgtcc aaatgggaag tcgctaccga caaataagat cagatcggct
     4621 tctgccaatg cttcatttcc gggtttagta gcgacacgtc cagcaaaacc aagaaagttt
     4681 tcataatcat caggaataat tccttttgct agaacggaag cgacaacagg aactgaaaaa
     4741 tgttctgaga attctttgat ttgcggaaat ccgtttcgcg ttccttgacc aacataaaga
     4801 actggttttt ttgctttttc aagataaggc aaagcagcta gcaaatcttt ttcttctggt
     4861 aatatcacac ttgttttgtg agtgtgagcg gtggagaaag gttgttcgtc aatttcttca
     4921 aaaccaagat caacagggat agtcacgata gccacgcctt tatgctcata ggcggcttta
     4981 atggcttcat ctactacatg cggaagactt tgtggtgtca tgactgtacg attgtatacg
     5041 ctaacatctg cgaaaatagg attttcattt agttcctgga aagagttata gttcatggat
     5101 gtagaagcaa cttgtcctaa gaacgctact agaggcacat ggtccatctg tgcatcatac
     5161 aaaccattga tcaaatgtgt cgcaccaggt cctgcagagc cgaagacagc gccgactttc
     5221 cccgttaatt ttgcatctgc agcggctgcg agtgcgccga cttcttcatg acgaacttga
     5281 atgtagttga tttcggtttt ttcttgatat agcgcatcca taattgaatt gaaagatcct
     5341 ccgggaatgc cgtaaatatg gtcgataccc cagctttcaa gaacttttac cattgcgatt
     5401 cctgcattga tttttgacat ggtgtttcac tcctttagca atgatgatac ataaattttt
     5461 tgtatctaga gtatagaact ttttatcgaa aatcccaact aattcgtctt ttcacaaaaa
     5521 tgtagtcatg tttttgggaa tatgctagag tagtacatag atagattcaa gagaggggaa
     5581 tggcatggtt gaatggctat tgagacttga accttggcag caagcacttg ctgggacagc
     5641 ttttacttat tttatgacag ctcttggtgc cgggttggtt tttttcttta aagaaatcaa
     5701 aaaagaagtg ctaaacttga tgttaggatt tgcttctggt gtcatgattg ctgcaagttt
     5761 ttggtctttg ttagatccgg ctattacaaa agcagaagaa aatggagata ttgcttggct
     5821 agttgtaagt attgggtttg gtttaggcgg tgtgttttta tatatggcag ataaaacact
     5881 gccccatatg cattttggac cacaacatga agcagaagga ttgcctaccc atttaaaacg
     5941 gacgatcttg cttgtttttt ctattacatt gcataacatt cctgaaggtt tagccgtggg
     6001 ggtagcattc ggtgcagcag caactgcgga taatccgaca gccgctatat tagccgctgt
     6061 ttcggtggcg ctaggtattg gtatccaaaa cttcccagaa ggagcagctg tatcaatccc
     6121 gttgcgacaa gaaggtctga gtcgtaaaaa ggcctttgtt tacggacaag cttctggaat
     6181 cgttgaacca attgctggag tgattggagc gctactagta acgaaagtgg agctgctgct
     6241 tccttatgca cttgcttttg ccgcaggagc aatgatttat gtcgtggtgg aagaattgat
     6301 ccctgaagca cagcagacat tgtccagcaa acgtcatttt gcagtttttg gcgtcatgag
     6361 tggatttatc attatgatga ttttggatgt ggcattagga taaataaaaa aaatatatat
     6421 gatatatcaa atagggccgc gaacttatat cgcgacccta tttttttagt aagttatatc
     6481 ataatgaagt gaatggtatc atacattaat aatagttttt tctatgtgta tttcctttga
     6541 tccagcgaac aagccagatg ataagtgcga ttgggattag aactttgacc gcaaaccata
     6601 aaagcccgct taggacactg acaaaaagac tgatgacgaa gctagctgca attagcacta
     6661 aaagaaccat gaagatattt aagaacatat ttttttgacg catacaaaac cctcctcttg
     6721 ttcattcttt actatagaaa aagtataaca gggattgttt aaaaacacct ccaactgaag
     6781 actgattttt acttcatcta cttaaatgat caaacaattg ctttttctct ggtttatcat
     6841 ctaaaataga gcggaaaagg aggagaatat catgcctaaa aatcgtcttg aagcatttac
     6901 agatgctgta atcgcgatta tcatgactat tttagtttta gaacttcatg aacctaacgg
     6961 agacacgtgg gaggcgctcg ctgcattagg ttaccgattt tttatctata ttgtcagttt
     7021 tatcatttta gcgatttatt ggaacaatca tcatcattta tttcaagttg tacataagat
     7081 aaatggacgg gtcttatggg cgaacagttt ctttgttttt gcgctttcgc tttttccgtt
     7141 tgcaacttca tggatcagtg agtacatcaa tagcctggct ccagaaatga cttatggttt
     7201 tgttaccttg accgctaata ttgcgtatta tatcttggca aaagaacttg tcaaagcaaa
     7261 taaaggccac gagaaagcat tgaaggcgat gttgaataat tacaagaaat cgtatctatc
     7321 gattggtttg aatgtgttag gaattgtctt agggtattta gtagcgccag tagccgtgct
     7381 gatcgcaaac atcgtgattc tagctgcttg gatcatccca agtcgtacca tcgaatcaca
     7441 atatcggtaa aatgatccgt gtttaaaatt taagagaaaa atatagaaaa aataaataaa
     7501 cgatcaaata tccgagtgat actagtattc aaagagggat gcagatggat caaaaaagaa
     7561 ttcatgtagg actgattact agttttgtca cgatgcttat ttatttggat caaggtcatt
     7621 tgacttggac agtagttggt gtcgtaggat ttttgacatt attcacagca ataaaaaaat
     7681 aaacatcttt atgtaatctg atgttctatc tatttaagaa aagcatgttt acagcagtat
     7741 gattctgttg taaacgtgtt tttatttgtg gaaaatttaa tttttagaaa agtgtatcga
     7801 aaaatagata gttagttttt ccacagtgga taacttcgta agagataaaa ataaaaaatt
     7861 agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa atgaataaat
     7921 attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa aaataacata
     7981 tctcatttta cgcttttgtt attttatact taaaaaataa cgacacagaa aataccgtgt
     8041 ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa
     8101 agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa
     8161 aaaccataga aaatataaag taattactta taaaacaagc tttttataga aaaataatat
     8221 acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa
     8281 gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa
     8341 tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga
     8401 tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg
     8461 aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc
     8521 aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga
     8581 cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc
     8641 agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg
     8701 aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa
     8761 gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta
     8821 taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga
     8881 ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa
     8941 atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata
     9001 gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta
     9061 tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa
     9121 tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc
     9181 tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt
     9241 gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg
     9301 gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg
     9361 ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat catgaatctg
     9421 atgctactta tctttattgt attatggtta ggttacataa atcatgattt tttgatttca
     9481 agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat
     9541 ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc
     9601 ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc
     9661 agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt
     9721 tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat
     9781 ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca
     9841 cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta
     9901 gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac
     9961 ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca
    10021 gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg
    10081 ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg
    10141 atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat
    10201 atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt
    10261 tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt
    10321 gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa
    10381 tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa
    10441 ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa
    10501 aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttatatt tattctatca
    10561 aaaaattttg tttatgatgg taactgaata aactttcctg agatggtcaa tgctttcctt
    10621 gaaagtctta cagatattct ttcttcatag aaagtttaag ttttatagtt agtgtacaat
    10681 aaaagtatgt ttataccata tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa
    10741 agatatgaaa aggggaaatt tgatgaagaa aataagcaat gaaatccaaa ttctggcaga
    10801 aactttaact cttcccaaag tcgttataga gcaaatgatt gtacaagcaa aaaacatcct
    10861 tgatgaggtg tttgagcaat atagtcatga actaatggat cctaataaag caaaggcgac
    10921 tgcaaagaaa atagacaaag tctatgaaga ggaacctttt gcagcattag ctatttatct
    10981 atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc caagaactat
    11041 ctatttggca acaatgaatg cttttactag atttattcat gaacatttcc aagtaacagg
    11101 aagctatcgc tttgaccgtg gtttttggat ttggcattac actagtggct tgatctttcg
    11161 gattggtgaa ttggagtacg aaatgacata ttttccgaaa gggtacacgg atctaagatt
    11221 agaaggaaag cagtgccttt ctcttcatat tccttccgat gcagatttat ctcagaataa
    11281 gataagagat agttacgaaa gtgccattcg tttttttgaa ctttattttc ctgattacca
    11341 ttaccaagtg atgtacacag atacttggtt actatcaccc aatcttacta aatggttgaa
    11401 aaaagagtca aaaatctgtc tttttgcagc cgattatcga ttattatcag ttgatgaaca
    11461 ggacgatagt ggagtccctt ggatctttgg acgagtagat gcccaaatac atgattatcc
    11521 agaaagtacg agtttacagc gacaagcaaa agagcaattg cttgcaggcg agcacattgg
    11581 ctctggctta ggtattctta cttcataagt ttagtacttc gaaatagttt aaaagagcgt
    11641 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    11701 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga
    11761 atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt agtgttctat
    11821 cgaacacaca gtacgttcaa taggtaaact agctataaca atagatatta aagttaacgg
    11881 acaagagggc cttttttagg tagctaaaac gtaataatga aaatttattt ccagagatag
    11941 gagtcaatca gctagtctga tcgactgttt taacaagttg actttcattt taacttttga
    12001 taccatttaa aacacaaatc gtaattgttt cgatttgtgt tttttagcat gactacaatc
    12061 aaagaagaaa gtgggtcgtc catatggaaa aaacagatat ctcaagtgca taccgccgct
    12121 tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac
    12181 ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt
    12241 tttcggctat tttcaataat cggtttttgt gtaaggtttc cgaaagaact gctgttgatt
    12301 ttccattttt aacatatgat aaattggtag aatctttctt ttcaaattga taccattccc
    12361 taattccagt tatatagtta actacagaac cacaaagcac gtacgtttat aaaagtatta
    12421 tctgtagtta tttttcaatt ccctttcaac ctcttatttt gaccagtagt attgatcact
    12481 ttacctttta acacctttgt tcgtttttga aacattttta gtcaagaatg gaaacgtaac
    12541 tacataatca aaggttttgc gaaatatttc atgaaatatt tcatgtagaa gcgagcaata
    12601 cggttgcaaa taaccattat cgtttaataa gcagttacga aagctcccaa tatctctaac
    12661 gttcagctca ctccatttct tttcttcaaa aaaatcagtg attcgtatct ctttctattt
    12721 gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca
    12781 gttactattg ctagaataac atcaaggaat aataagtttt ggttttcaga taatagtcga
    12841 taagttgtgt attggaattt tagatacagt aataaagtat ttttcatgaa attttgtgag
    12901 aatcactatt acaagtatct ttaataaaat taatggttaa ggaggaactt aaagtagaat
    12961 gtaaatgaat agttgtagtc agtcgattgt gatactagac tcaaagttgc aggaataagt
    13021 ttttcaattt gtgattggaa gataaaaaat gaaaaagaag ggcttttttt tatactttgt
    13081 tgattgtagt gtttattttc cctctgttta gccaagtaac attttctatg actcaaccag
    13141 tttcaagtga ggaacatgat aacctttttc gtgctacaga agagtttaag aaatatcagg
    13201 aaaatattgt tccggagact ccattagcta gtgagccaat tactgatatt gatggaaacc
    13261 aaataggatg gtatatacag tacgaagtta tggtggtaaa taatgactca atagtaaaaa
    13321 atacagtagg cttagagcta acaccgattg aaaaggtgag ttcattgtta acgtatattt
    13381 atactgatga aagtgacgag actgagatca ttttaacgga ttactcaaaa attgctactg
    13441 ataaaaagta tatattgtgg atgtagacac tagtgaaata ctttatgata tagaaattga
    13501 tgaaaattta ctagatgtag cacaagaaat cgatcatcaa aagcaggaag ctattcgata
    13561 tgctggtaga aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa gttagagaag
    13621 atgaaaatga gtggaaagaa ttcaagagtc gctactcaat taactcgact ccgacattta
    13681 cagtttacag agaaggatcg atagaaaaaa ctgttttttg gactaaagaa agtggaatgt
    13741 ctttagctga agtagaagag tttttggatt atgttagtat gcaacagtaa gggagttctt
    13801 attatgaaaa attttgacac atattatgtt attgtttttt cgatatcttt gctgttaata
    13861 ttactaactt taatgcagta taaagagtat ccagagtatt ttattttaac ttttgtatat
    13921 ttagtaagta gctacacact tctatataaa ttcagaagtt cttctagagt ttctaaagtt
    13981 tgtttcttaa ttagcttatt gattctataa tatttttgat ttatatagga aattaaaaat
    14041 attatagaat caacactcag agaacgggtt ggtttttaat agatttaata agtactgata
    14101 atgaaatttc tcctggttgg gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag
    14161 attactttgc ggagtatgcc gataggataa tgagagatta cgtaacagat aaaactcaag
    14221 aagtcatcaa tcaatcccag tcattaaatg aattatctga taaggcgaaa gaagaagcag
    14281 ttgccattat ttcttcaggg attagtgact atattgatat gagtggtgaa atcaattatg
    14341 gcgataaaga caaaattgta cgcatgataa aagcttttat caaaactttt gatgtgaagg
    14401 cagatcttaa gtttaatgat acttacctag taggaaaatt aattaatatc cttgggacag
    14461 agaaagatac ggaaacgttt atttcctctt tgtctatgtt gtttacagat aagcattttt
    14521 caactcagct tggtggagag gaactagtgg aaatctttta accattacgc ccagcattaa
    14581 taaaagtata tcaaaaaagt actcctgatg atttttaaga ttggtttgag aaataatgtg
    14641 aaaacactat ttttaatgtt aattatagat agagatgaac gcgtcatatg gttaaaagaa
    14701 tgaaaaagtc aaagatacac tgctatattt cactaaggat atagcagtgt atctttcatg
    14761 tcgtttcact ggcagttttt tataaaagga ggtgtgcatt tcagttgctt ttctttggta
    14821 gttagttgaa tcgtttaaga attaacggat gtggcagaca ggtgctctgt ccaaaaacca
    14881 gtaaaatctt tgatggttcc atctttattt agctgtgcct gttcaaatac tgttttgttt
    14941 ttaccgaatg gatcaattgc aataattgtt ccgatccccg cacgtttggc agcaagtaat
    15001 cctgagtaag catcttcgat aacaagacaa tcagccggag ctaagctcag tttgtctgcc
    15061 gctttaagaa aaatatccgg ttgcggtttc ccagggaaag agccgtcgtc atagacaatc
    15121 ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa aataaaaagc aacattctct
    15181 ttgacagtag ccgtggcaat cgttaatgga atctgttggt cagtcagctg gtcaagagtc
    15241 gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt gcaagcaaag catacgataa
    15301 tagctttctt tttcttctga caatacggcg atctcagagg cggtcaaatg ctctgaaatg
    15361 aaatgtttca aaatctccgt attcgtccga ccatgaatat tttttaaaat atcctcgtct
    15421 gataaaccat cttttgtatg atcatgtatc atatgcagcc aagcagcttc atgcagatga
    15481 gagtccaaaa acattgttcc gttgaaatca aatataatcc ctttcatagt tacgctccta
    15541 tagtggtatt ttcggttgaa tcagcaattt gcagatcgac atattgtgca tagtgctctt
    15601 gaacctcccg cgagatctgg cgatccgtaa tgagcagatc gaaatcatgc aaattataaa
    15661 acacataaaa atcatctcga ttgaattttt tattatcaac gagtagatat ttcttacggg
    15721 aattatttag agcgatctgc tgtgcttctc cttcttcggt actgtaagta gagatcgctt
    15781 cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa ctttagtctt cttaaattgt
    15841 cgttggtgat cggtccgaca aatgcgccgg tatttttccg gaaatcaccg ccaatcagta
    15901 gaatttctgg tccatctgct tgagcaagaa tatcaaaaac tggataatta tttgtaatga
    15961 tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc gattgttgta cctggtccta
    16021 agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt ggcagcaatt tctttttttt
    16081 cttgaatctg cagagtggct ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa
    16141 gactttgagc ccctccatgg acacgcagca gcttgcccga tttttctaat tcatctaaat
    16201 ctctgcgaac ggtcatatca gaaacatcta attgattgat gatttcatta acggtaagga
    16261 ttcctttttt attgaccatt tctacgatag taagcaatct ttcttttttc aacattgtct
    16321 tcacctctaa tttaaggata atctatcata taaacggaca acagtcaatc taattcgaac
    16381 aaaagtaaac atttagggtg gtttttatgt ttgttacaat agattttatt gctgttttct
    16441 ggtttttatg attatttgtg tttgtttttg ttgacaaatg ttttggatgc gtttacaatg
    16501 caatcagtta gaaagagcga aaactgatta tagatttgag gagagatgta catgcttaaa
    16561 ttaccagaag attttatttt tggcggagcc acagcagcct atcaggttga aggagccact
    16621 aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt tagaggaaca agggcgattc
    16681 agtcccgatc cggcaagtga tttttatcat caatatgcaa aggacattga attgtgtgaa
    16741 cgttttggtg taaatggtct aagattatcc attgcttgga gccgcatttt tcctgatgga
    16801 gctgggaaac caaatccaga aggaatcgct ttttatcatc gtgtgttcga agaatgtaag
    16861 aaaagaaacg taacgccgtt tgtgacgttg catcattttg atacccctaa acgtttgttt
    16921 gaccatggag attttttgaa tcgagaaaca atagaagcat ttgtttccta tgccattttt
    16981 tgttttcatg aattcaaaga agtcaaggtg tggagtacct ttaatgaaat ctatccagtt
    17041 gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa tcaaatatga ttttacaaag
    17101 atcgtcgcct gtcttcataa tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa
    17161 gaaaacgaac ttccgggtga aattggtgta gttcactcat tagagacgaa atatgcggca
    17221 acagacgcgc cggaagataa gcatgctgcc tttttagacg atgcattgtc gatccgtttc
    17281 ttattagatg ctacctattt aggctattat tcaactgaaa ccttgactgc tcttgatgag
    17341 atatgtgaag caaatcaggc cagctatcat tttcctgaag aagatttcgt tgaattgaaa
    17401 aaagcatcca cgcgtaatga ctacttaggg atcaatcact accaatgcca ttttgtcaaa
    17461 gcttacgatg gagaaaatgc aatccatcat aacggtacgg gtgaaaaagg gacctctgtt
    17521 tataaagtga agggaatcgg agaacgtatt tataaagaag gaattcctcg aactgattgg
    17581 gactggctga tttatccaga aggtctatat gacttgttgc tgcgaattaa aagcgattac
    17641 ccacattaca acaaaatcta tatcactgag aacgggatgg ggtataaaga tcagtttgaa
    17701 gatggcatta ttatggatca gccgagaatc gactatttga gagtctattt ggaatcctta
    17761 agcaaggcga taacagccgg ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat
    17821 cttttctcat ggacaaatgg gtataacaaa aggtatggct tgttttatgt tgattttgaa
    17881 acccagaagc gttacccaaa agaatcagct tactggtaca agttggtcag tgaaaccaaa
    17941 acgattattt aataagtgat aatggaggaa gaaaagatga ataaagagga attacaaatg
    18001 cttggttttg agatcgttgc atattcaggc gacgctcgca gtacattatt gaaattattg
    18061 aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg cgttgaaaga agccgacgaa
    18121 aatttaaccc ttgcacataa ttcgcaaaca aaaattttag cggaagaagc atctgggaaa
    18181 gagatggaga tgggattcat ttttatccat gggcaggatc atttaatgac cactttattg
    18241 ctgagagatc tgattcaaga ctttattgta ctgtacagac agaatcaagg ataggagaag
    18301 aacgctatgg atggaatcat taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc
    18361 tcacgtaata gttatttagg agccgttcgt gacggatttt taacagcgat gccagctatt
    18421 ttattttcaa gcattttcat tttgattgct gcggttcctg agatttttgg ttaccaatgg
    18481 cctgaatctg tctccacatg gctatggaaa gtctataatt attcgatggg tgttgtaggg
    18541 ttactcgttt cagcaaccac tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg
    18601 aaaaacaaag taatcaacac cacctctgtg atgctggcct ctgtggtaag tttcctgttg
    18661 ttaagcgtct ctccgattga aggtggattt tcaacggaat atatggggac aaagggacta
    18721 ctggcttcct ttgtcgctgc atttttaaca gttaatttat ataagttttg tgtgctgaaa
    18781 gatattacca ttaaaatgcc taaagaagtt cctggaacga tttcgcaaac cttccgagat
    18841 atttttcctt tctctttttc agtatttgcg gccgttttaa ttgatttagg cattcgacaa
    18901 atcttccaaa tctcatttgc agaagcaatc attacggtgc tgcagccgct atttacagca
    18961 gctgacgggt acttaggaat cgctattatc tggggagcga tggcgctgtt ttggtttgtc
    19021 ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg caattatcta tgccaatgtg
    19081 gaaaccaatc tgcaattgtt caattccggt gaacaagctt ctaaagtact gacggtgggt
    19141 ttagggaatt ttgtaggaac tatgggagga actggggcaa cattggttgt tccttttatc
    19201 ttcctactct ttgctaagtc taagcagttg aaggcagttg ggaaagcttc ctttattcca
    19261 gtaagtttcg ctgtcaacga accattgcta tttgcggcac ccatgatttt gaacccttat
    19321 ttttttattc catttttgtt aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc
    19381 gatgtattgg gcatgaacag ctttatgtac gttttgcctt gggcaactcc agcaccgatc
    19441 ggtttggttc taggaacggg catgggtatc ttagcttttg ttctagcagc ggttctaatc
    19501 gtcgttgact tcctgatcta tctaccattt tgcaaagcat acgatgctgt cttagtagag
    19561 aaagaagcac aaaaagcaga ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt
    19621 gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg caaataaaga aatcaatgtg
    19681 ttggtacttt gtgctggcgc agggacaagt gcgatgttag ctaatgcttt aaaaaaaggt
    19741 gcatctgaat cgaaagcacc gatcagtgca atggcgggtg cttatggttc tcattacgaa
    19801 atgctgcagg attttgatat ggtggtctta gcaccgcaag tcaattctta ctatgatgat
    19861 atcaaagaag acaccgatcg cttaggaatc aagctggttg cgacaaaagg agcagaatat
    19921 atcggattaa cgagagatcc tgaaaaagct gttcagttta ttctgaacca atttcaataa
    19981 aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt
    20041 gttaggggct ttgggggctg tttcaaaaac tgttgaagca tctgccagcg aatttattcg
    20101 cggagcagat atttcgatcc tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga
    20161 cgatatttca aaagatgcct tagagatttt aaaaaataat ggtgtcaact atgttcgact
    20221 gcgtttgtgg caggatcctt ataatagtaa tggagaaagt tatggagcag ggacaaatga
    20281 tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct
    20341 ggattttcac tacagtgatt tttgggttga tcctgggaaa cagaatcttc ctaaagcatg
    20401 gcaaggtctg acctttgaag aaatgaatac ggcattgtac gactatacaa agaacgttct
    20461 gtcggaaatg aagcagctgg acgtttatcc tgatatggtt cagatcggca atgaactgaa
    20521 tagcggaatg ctctggcctt atggcaagtc ttggggagaa ggcgggggag aatttgatcg
    20581 attggctgct tttttgaagt ctggtatcca agctgttcga gacactcaac ctaaaacaac
    20641 tccagtaatg ctccacttag ctgatggtgg cgatacagga gcatttactt ggtggtttga
    20701 tgaaatcacg agtcgaggtg tgtcatttga tttaattgga gtgtcgtatt atccgtattg
    20761 gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa
    20821 agaagtgatc gtagttgaaa cagcttatgg gcatacaaca gcgaatgccg atactatgcc
    20881 aaatgctttt ggtgaagccg aagcagctgc ggggggatat gaaccaacac cgacaggaca
    20941 agcagagtat ttgctggatc ttgccgatcg gattcaggca gtaccgaata atcgcggggc
    21001 gggcttcttc tattgggaac ctctttggta caacggaaat gtgagttggg caacacaagc
    21061 aggtatgaat tatctcagtg ttcaatccac tatgggaaac gaatgggaca accaagcgat
    21121 gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg tttaaacaag caggggcgca
    21181 aataaatcta gttaaaaatg caagtttcga aagcgatgga ccaacgacca gtccttccaa
    21241 ttggcaaacc tggtttcaac aaggaaccgc taagggtacg atcaaaacgg aagcgggcac
    21301 tttttttggg gactacaaac taactttttg gcaggatcaa gcatacgagg catcagctta
    21361 tcaaaatgtt agtggcataa gcaaaggaac ttacacattg agtgcctatg taatgtctgg
    21421 gggagagttt gatactaatc agttatatat caaaaattat ggtggagcgg aactaaatac
    21481 tgcggttgtt aaaacaagtg aatggacaaa aattacaatc aaagacattc caattacaac
    21541 aggtgtttgt gaaattgggg tttatagcaa ggcttcagga aatgcttgga cgaacgttga
    21601 ttttgtttca ttggtgaaac aatagactgc ggatcgtttc gattcaagat cggaggttcc
    21661 cttcagccac atcgttagcg tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa
    21721 aataaccaga aacgatttcc agtcattttt accaaaacga gtaaagcacc agacttcatc
    21781 tacactttgt attatgctaa agagagttca acgatttcgt aagaagcaat gaaaaaattg
    21841 caaaaagttg tgcagataac ataaagtttg tttaagaaag ggtaacctta gaattccgtc
    21901 gtgaatatga tttgagcact tagatggata gaaaaaagtt ctcggaaaac agttcgtttt
    21961 tcgagaactt ctttttatga taagaatgaa acaaattaac ttatattata gtctcttttg
    22021 gtagggaacc atcttatttt taatgaaaat caagcatttt agctattgaa gaatgttgat
    22081 ttatagggat tccccctcca tattatgttg gctctttatt cccactctgt tttcaacgta
    22141 aagtgtataa aacagaatgc gatactatca agggagttaa aacaatatac agaacagtaa
    22201 aacactataa ataaaaataa aagtgcaata aaagtgcaat ttttgcacct aatcatactc
    22261 tgtttatata tgaaaactag attctgaatc caatatttgg ttaacgagag gcgttcgaat
    22321 ttgaacgtct tttatatatt actttcaaat aaatatgagg tagaatacta tctattttct
    22381 ttttaaccca attgttataa tggagagtgt aaaatatttt gtgtaaatag aaaaaaggaa
    22441 gtcccttctg tagaatagag ttaccacaac acattcacag aagagaggac ttccctatga
    22501 acgattttac tacagaaatt ctaaagactc tagcgaacaa aggcgatttg aatgaattat
    22561 tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa aacggagtta acggctttcc
    22621 tagattacga aaagtatgat cgcattggtt ttaacacggg taattctcgt aacggctcct
    22681 atgaccgtac ggtcaagacc gagtatgggg aacttcatct ccagattccg cgcgaccgca
    22741 atggtgagtt caagcaacag actgttcctg cttatagacg gacgaatgac acgttagagg
    22801 agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc ggaaatcgca gacttgattg
    22861 agaaaatgta tgggcattac tactcgcccc aaaccatgtc caatataaca aaatcattta
    22921 cagaagaggt aacggcgttt aatgggcggg agcttcatga ccgttatgct gctatttata
    22981 tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc caaggaagct attcatatcg
    23041 cagttggcat tcgcccggac ggatcaaagg aagtattgag ctatgcgatt gcaccgactg
    23101 aatccatcac gatttgggag gaaattttat tggaccttca agagcgcggt ttgaaaaatg
    23161 tcctcctgtt catcacggat ggcttaaagg ggatggtagg agcgattagt cggttctatc
    23221 ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg taatatcagt cataaagtgc
    23281 gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat ggtgtatcaa gcctcatcta
    23341 aagaggtggc gttggaagca cgtggtactt ttgcggaaaa atggaaaacc agctatccaa
    23401 aagtggttga atcgattctt tcgaacgatc acttgctcac tttctatgat ttccccttgg
    23461 ccatacgcaa gagtatttat tctacgaact tgattgaatc ctttaataaa caaatcaaga
    23521 aatacagcca ccgcaaggaa cagttccaaa atgaagagtc gatggaacgt ttcttagtct
    23581 cgtcttttga tacttacaac caaaaattcc taggtcgcag tcataaaggt ttccaacagg
    23641 ccgaaggcga gctcgaacaa atgctaagcc aactgattga gaattagaaa caatctgcag
    23701 tagggaagaa tcatttacac aaaattattg acgctccctt ataatgagaa cttttactaa
    23761 cataatttat gataaactta ctatataatc atattaatta aatagttagg agatctgctt
    23821 atgtttatat ctactcttca aattcgaaat tttagaaatt atgaatccga aatctttcga
    23881 ttcgataatg agacaaatac tattataggt gagaatgatt cagggaaaac caatgctctt
    23941 actgcattga gaatcttatt agatgatagt tattactata gtagtaaaac actaaaagaa
    24001 tcagattttt ttcatggaat tcaaaatggg tggcaagggc attggattat tatctctgcg
    24061 acatttgaag gaatcagcga agaagaattt gacaatgaga tttgtgcttc catttcttta
    24121 aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa atgcagataa aggtgtaggt
    24181 agtatttctc tatttattag acctaacaaa gctattagaa agcaattatt tgatatttcc
    24241 actgaattag actctcatca atttaacgaa tttagaaatt ctattcgtct atccgactat
    24301 gaattttact acacatctaa atcaaatcta aatttctgta tcaatgaaaa ttatgataaa
    24361 ttggtaggaa gactcaatga caatatagct agtaatcctg aagaagacga tgaagctctg
    24421 ttgggctcaa gaattgacat gtcagatatt ttcaagcata tttcggttgt ttatgttgac
    24481 gctcttcgag atgttcttag agaaatgaaa aacaatcgaa acccagtcaa aagaatcatg
    24541 gaaacaattg aatcaaaaat atcatcagat aatgttgagt cattaaaaac aattatacag
    24601 caattaaatg agactattac cagtgttcct gaaattagga aaattggcga aaatattaat
    24661 cggcaactaa atagcataat tggttcagtg tattctccta atcttcttct ttcctcaact
    24721 atgtcagatg atatgggttc tttagcaaaa tttctttcaa tgaagccaga acaaaatatc
    24781 gacttagact tactagggtt aggacatcta aatatgattt atttagcttt gaaaatagtc
    24841 gaatttgaag cgtgtcgttc aagagaacta cttaatatta tgttgattga agaaccagaa
    24901 gctcatattc atcatcatat tcaaaaaact ctgttcgaag gattaaatct tcaaaaaaac
    24961 tatactcaga ttctcatgac cacacattct gtacatttag ctgaagtgtc tgaaatatct
    25021 cggatgaatg tattaaaatc gtttaatggc aaatctatcg ttatgaatcc agcaaataat
    25081 ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc taaatctaat aaacgcagta
    25141 gagagatatt tagatgttaa aagaaatgga ttgctttttt ctaaaggtgt tatcttagtt
    25201 gaaggagatg ctgaagaaat acttattcct caaatagtaa aaaaattatt tggtataaac
    25261 ttagacgaac ttggaatagg tttgattaat gttggtagta cttcgtttga atatatcgct
    25321 agcttgtttg acgatgatag aatacagaga aagtgtgcta ttgtaactga tttagatgaa
    25381 caggcaatcc catcagaaca tcgattgtat aaaagcactg cacaagagaa aggtcaagca
    25441 agaaaagaaa aattatcctc cctttttaat gaaaacaatt gggttaattg tttttatgct
    25501 aatacaacat ttgaaattga gtttttagat gtgaataaag aaaatattct aacttatgtc
    25561 agtccaatta tagatgacgc attcaaagat aaaaaaacaa taactgctta taaagaagaa
    25621 atacaaagtg aacaatattt agaaagaaat ttagcgatgt taaaactagc caatcatgtt
    25681 ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg actttttaat caaaattcct
    25741 aagtatattc ttaatgctat tgcttttgca tgtcaggaaa tagtagatct ctcaatttat
    25801 aaaaaaattc tactccatac aatcgacttg tacgaggaaa ctacagaatt gtcagcttta
    25861 tcagctagtt taaaaacaag tgatacagct gatttgaata tagcaataat ttctttatta
    25921 gagtactttg gtaaagatga ggatgttgtg tatctaatca atcagataga aaattacaca
    25981 aaaatacttt atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc gtaaatgatt
    26041 taaatttaga acaaaaagat gttgtggaaa gtgaagataa cctttatgta aatgcatgtc
    26101 ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca attaaacacg
    26161 atggatcact aaaaagaata attgctatca catacactaa tagagctgct gaagaaatta
    26221 aggaacgcct aagtttactt agtatagatg atgatattaa tatttgggtg gggacaatac
    26281 atcagttttg tcttgaattt attatttatc cttttaaaat gaatctctca agagtatcaa
    26341 aaggatttac aattattgat gattatactc aaagagctta cgtcagccaa attaataata
    26401 tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca ctttctcatg
    26461 attttaaaat taatgaagtt cagtatactc aagtttctga aatgtatcac actatgttgg
    26521 aaaaaaataa agaaattgat tttgatatga ttttactagt agcgtataaa attttaactg
    26581 taaacagtaa cgttgcacgt aatattgcta gtaatataag ggcgatttat gtggatgaat
    26641 ttcaagatac tagagaatta caatacaatg taatagctaa attacttcaa aacaaccctc
    26701 aaattcagtc aatgtttgtt ggagatattg accaagcaat ttatggatca ttagatggta
    26761 tagctaaatc agttgaagaa ctagaaatgc taactagtca cacttttcaa tcaaagacgc
    26821 tacatggctg ttacaggtct aatcagagat tagtagactt ctactctaat tttcagagtt
    26881 gtccttacga gattgaaagc agagggaata ataaaaacga ccgtggtttt ataagttatg
    26941 attgcaaagt tactaaggag gatttaccca acataataaa aaatataata aaggaaaaga
    27001 ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc tcctcaacac tatcctctgt
    27061 tttcactggg tgagtattta aagaaagaat taccgtattg caattttgat gctataaata
    27121 tatcaccaat taaggtaaat aatcatagtt tatttttcaa gttagcaatt ttgtacttta
    27181 ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc aaacgatatt ttaattatat
    27241 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
    27301 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
    27361 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
    27421 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
    27481 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
    27541 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
    27601 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
    27661 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
    27721 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
    27781 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
    27841 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
    27901 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
    27961 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
    28021 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
    28081 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
    28141 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
    28201 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
    28261 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
    28321 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
    28381 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
    28441 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
    28501 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
    28561 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
    28621 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
    28681 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
    28741 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
    28801 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
    28861 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
    28921 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
    28981 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
    29041 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
    29101 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
    29161 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
    29221 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
    29281 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
    29341 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
    29401 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
    29461 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
    29521 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
    29581 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
    29641 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
    29701 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
    29761 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
    29821 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
    29881 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
    29941 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
    30001 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
    30061 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
    30121 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
    30181 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
    30241 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
    30301 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
    30361 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
    30421 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
    30481 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
    30541 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
    30601 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
    30661 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
    30721 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
    30781 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
    30841 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
    30901 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
    30961 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
    31021 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
    31081 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
    31141 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
    31201 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
    31261 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
    31321 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
    31381 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
    31441 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
    31501 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
    31561 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
    31621 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
    31681 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
    31741 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
    31801 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
    31861 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
    31921 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
    31981 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
    32041 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
    32101 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
    32161 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
    32221 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
    32281 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
    32341 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
    32401 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
    32461 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
    32521 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
    32581 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
    32641 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
    32701 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
    32761 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
    32821 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
    32881 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
    32941 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
    33001 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
    33061 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
    33121 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
    33181 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
    33241 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
    33301 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
    33361 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
    33421 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
    33481 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
    33541 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
    33601 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
    33661 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
    33721 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
    33781 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
    33841 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
    33901 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
    33961 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
    34021 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
    34081 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
    34141 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
    34201 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
    34261 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
    34321 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
    34381 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
    34441 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
    34501 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
    34561 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
    34621 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
    34681 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
    34741 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
    34801 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
    34861 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
    34921 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
    34981 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
    35041 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
    35101 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
    35161 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
    35221 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
    35281 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
    35341 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
    35401 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
    35461 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
    35521 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
    35581 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
    35641 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
    35701 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
    35761 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
    35821 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
    35881 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
    35941 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
    36001 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
    36061 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
    36121 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
    36181 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
    36241 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
    36301 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
    36361 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
    36421 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
    36481 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
    36541 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
    36601 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
    36661 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
    36721 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
    36781 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
    36841 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
    36901 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
    36961 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
    37021 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
    37081 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
    37141 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
    37201 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    37261 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    37321 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    37381 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    37441 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    37501 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    37561 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    37621 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    37681 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    37741 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    37801 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    37861 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    37921 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    37981 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    38041 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    38101 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    38161 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    38221 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    38281 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    38341 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    38401 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    38461 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    38521 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    38581 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    38641 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    38701 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    38761 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    38821 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    38881 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    38941 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    39001 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    39061 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    39121 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    39181 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    39241 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    39301 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    39361 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    39421 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    39481 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    39541 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    39601 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    39661 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    39721 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    39781 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    39841 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    39901 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    39961 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    40021 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    40081 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    40141 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    40201 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    40261 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    40321 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    40381 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    40441 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    40501 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    40561 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    40621 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    40681 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    40741 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    40801 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    40861 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    40921 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    40981 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    41041 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    41101 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    41161 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    41221 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    41281 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    41341 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    41401 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    41461 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    41521 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    41581 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    41641 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    41701 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    41761 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    41821 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    41881 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    41941 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    42001 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca
    42061 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
    42121 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
    42181 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    42241 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    42301 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    42361 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    42421 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    42481 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    42541 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    42601 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    42661 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    42721 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
    42781 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
    42841 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
    42901 aattgaatat acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt tttggagtac
    42961 aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt
    43021 tttattgtta aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat
    43081 ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt
    43141 acaatttact aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa
    43201 gaacgctaag tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata
    43261 cgctgaatat gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt
    43321 agacgataag aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga
    43381 aaaaatgggt atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc
    43441 cacgaaggaa gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga
    43501 tagggctaaa caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat
    43561 gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta
    43621 ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa
    43681 agggaaacca agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat
    43741 gtactgcaat gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt
    43801 atctaattgg tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga
    43861 tgttgagcca gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt
    43921 caatgagccg aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca
    43981 atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta
    44041 tcaccaacaa aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc
    44101 agtcagtcgt acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga
    44161 gtggctgtat aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac
    44221 aaagaaattt aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt
    44281 ccgtaagaaa ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac
    44341 gtatatagtt gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta
    44401 tgattggaat atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca
    44461 tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc
    44521 atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa
    44581 taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta
    44641 ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt
    44701 ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca
    44761 tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg
    44821 acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct
    44881 acggactttg gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga
    44941 ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc
    45001 attagccact aaggataaag gattttattc agttgattgt agaacggatt tatctgctct
    45061 ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt
    45121 tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg
    45181 tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac
    45241 aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt
    45301 gacacctgct caaagattag ggattacaga taaacaattt gattggaaag atataattta
    45361 tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt
    45421 cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata
    45481 gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc
    45541 ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat
    45601 gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa
    45661 aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca
    45721 accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc
    45781 tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt
    45841 tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac
    45901 tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac
    45961 taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt
    46021 agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt
    46081 ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg
    46141 taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa
    46201 actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt tacgagattc
    46261 ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg
    46321 tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt
    46381 tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga
    46441 aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat
    46501 gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga
    46561 ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag
    46621 tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta
    46681 tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc
    46741 tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta
    46801 tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg
    46861 gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg
    46921 ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac
    46981 tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct
    47041 tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa
    47101 atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga
    47161 aattcctgct cgatgtattc aagattatcg caatgattta acttttttta caaatgggct
    47221 tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca
    47281 gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta
    47341 gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta
    47401 atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat
    47461 tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa
    47521 atttcccttt atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga
    47581 atataataac aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa
    47641 actctcatct tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt
    47701 cttgcttcat tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag
    47761 ctatccatgt atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt
    47821 aagaaaaatt catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta
    47881 cttgggtgtt gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt
    47941 gttcccactt gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca
    48001 acggctctaa aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt
    48061 tactgttcct atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc
    48121 ctatagaaca ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct
    48181 gattttattt acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa
    48241 ttggcgactt aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct
    48301 taattaagtt cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg
    48361 tggttttgca gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt
    48421 ttatgtgctt ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac
    48481 aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc
    48541 ctttcacgac agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga
    48601 aaatcagttt gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa
    48661 acatctagac agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt
    48721 caactctttt tctactgttg atacgtacgt cactgatttc caactattta aattatttgg
    48781 attaagaatc aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa
    48841 aaaacggaac attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga
    48901 gttactaaat ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat
    48961 taaagaattc atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc
    49021 ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat
    49081 cgagggtata cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat
    49141 atggttattg atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta
    49201 tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt
    49261 cccgatttgg gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata
    49321 ggctaacaag ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa
    49381 gaaaagagct gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag
    49441 taattcgaaa gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata
    49501 ctcccgtata gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg
    49561 agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata
    49621 ttcttctctt gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg
    49681 agttttccta gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt
    49741 tagttgagct aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat
    49801 tgatgattac aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg
    49861 aagctgttga agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt
    49921 tgcacagtaa ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac
    49981 ctattatttt cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg
    50041 tagtggattc actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa
    50101 accagaagaa ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat
    50161 tttccttcca tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac
    50221 tattaagaag catttctctt attttgataa aatggctgtt tccgatattg agcctattga
    50281 tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt
    50341 tcaaggacta ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa
    50401 tccatctaag attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa
    50461 agaagaattt gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt
    50521 atttatctct ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat
    50581 tcaatgggaa gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta
    50641 caagaatcaa tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat
    50701 tgtattagat aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct
    50761 aattcagaca gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc
    50821 atatgctatt gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt
    50881 acggcattct catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga
    50941 tcgtctagga cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa
    51001 cagtaatttc gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca
    51061 aaattatgat acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat
    51121 gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa
    51181 gaggat
//
LOCUS       DACIFS010000015        48678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.015, whole genome shotgun sequence.
ACCESSION   DACIFS010000015 DACIFS010000000
VERSION     DACIFS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.015"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(148..2301)
                     /locus_tag="IYA83_000860"
     CDS             complement(148..2301)
                     /locus_tag="IYA83_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ8586312.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="IYA83_000861"
     CDS             complement(2595..3443)
                     /locus_tag="IYA83_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ8586313.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IYA83_000862"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="IYA83_000862"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ8586314.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="IYA83_000863"
     CDS             complement(5944..6123)
                     /locus_tag="IYA83_000863"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ8586315.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IYA83_000864"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="IYA83_000864"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ8586316.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="IYA83_000865"
     CDS             8271..10550
                     /locus_tag="IYA83_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8586317.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10643..11290)
                     /locus_tag="IYA83_000866"
     CDS             complement(10643..11290)
                     /locus_tag="IYA83_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586318.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11469..12188)
                     /locus_tag="IYA83_000867"
     CDS             complement(11469..12188)
                     /locus_tag="IYA83_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ8586319.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12319..13470)
                     /locus_tag="IYA83_000868"
     CDS             complement(12319..13470)
                     /locus_tag="IYA83_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ8586320.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13687..14427)
                     /locus_tag="IYA83_000869"
     CDS             complement(13687..14427)
                     /locus_tag="IYA83_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8586321.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IYA83_000870"
     CDS             complement(14431..14754)
                     /gene="rsfS"
                     /locus_tag="IYA83_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ8586322.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14811..15404)
                     /locus_tag="IYA83_000871"
     CDS             complement(14811..15404)
                     /locus_tag="IYA83_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586323.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15388..16035)
                     /locus_tag="IYA83_000872"
     CDS             complement(15388..16035)
                     /locus_tag="IYA83_000872"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ8586324.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IYA83_000873"
     CDS             complement(16048..16359)
                     /gene="yhbY"
                     /locus_tag="IYA83_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ8586325.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IYA83_000874"
     CDS             complement(16375..17484)
                     /gene="yqeH"
                     /locus_tag="IYA83_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ8586326.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17481..18008)
                     /locus_tag="IYA83_000875"
     CDS             complement(17481..18008)
                     /locus_tag="IYA83_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ8586327.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18188..18973)
                     /locus_tag="IYA83_000876"
     CDS             complement(18188..18973)
                     /locus_tag="IYA83_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ8586328.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18966..19835)
                     /locus_tag="IYA83_000877"
     CDS             complement(18966..19835)
                     /locus_tag="IYA83_000877"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ8586329.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19836..21215)
                     /locus_tag="IYA83_000878"
     CDS             complement(19836..21215)
                     /locus_tag="IYA83_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ8586330.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IYA83_000879"
     CDS             complement(21223..21642)
                     /gene="fabZ"
                     /locus_tag="IYA83_000879"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ8586331.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IYA83_000880"
     CDS             complement(21667..22143)
                     /gene="accB"
                     /locus_tag="IYA83_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ8586332.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IYA83_000881"
     CDS             complement(22146..23381)
                     /gene="fabF"
                     /locus_tag="IYA83_000881"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ8586333.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IYA83_000882"
     CDS             complement(23400..24137)
                     /gene="fabG"
                     /locus_tag="IYA83_000882"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ8586334.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IYA83_000883"
     CDS             complement(24149..25066)
                     /gene="fabD"
                     /locus_tag="IYA83_000883"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ8586335.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25173..25397)
                     /locus_tag="IYA83_000884"
     CDS             complement(25173..25397)
                     /locus_tag="IYA83_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ8586336.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25450..26412)
                     /locus_tag="IYA83_000885"
     CDS             complement(25450..26412)
                     /locus_tag="IYA83_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ8586337.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26422..26862)
                     /locus_tag="IYA83_000886"
     CDS             complement(26422..26862)
                     /locus_tag="IYA83_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8586338.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27168..28133
                     /locus_tag="IYA83_000887"
     CDS             27168..28133
                     /locus_tag="IYA83_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ8586339.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28190..28420)
                     /locus_tag="IYA83_000888"
     CDS             complement(28190..28420)
                     /locus_tag="IYA83_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ8586340.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28506..29393)
                     /locus_tag="IYA83_000889"
     CDS             complement(28506..29393)
                     /locus_tag="IYA83_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ8586341.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29482..30705)
                     /locus_tag="IYA83_000890"
     CDS             complement(29482..30705)
                     /locus_tag="IYA83_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ8586342.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30722..31801)
                     /locus_tag="IYA83_000891"
     CDS             complement(30722..31801)
                     /locus_tag="IYA83_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ8586343.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IYA83_000892"
     CDS             complement(31932..33659)
                     /gene="ptsP"
                     /locus_tag="IYA83_000892"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ8586344.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33659..33925)
                     /locus_tag="IYA83_000893"
     CDS             complement(33659..33925)
                     /locus_tag="IYA83_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ8586345.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34404..36638
                     /locus_tag="IYA83_000894"
     CDS             34404..36638
                     /locus_tag="IYA83_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ8586346.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36786..37106
                     /locus_tag="IYA83_000895"
     CDS             36786..37106
                     /locus_tag="IYA83_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ8586347.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37311..37535)
                     /locus_tag="IYA83_000896"
     CDS             complement(37311..37535)
                     /locus_tag="IYA83_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ8586348.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37733..39313)
                     /locus_tag="IYA83_000897"
     CDS             complement(37733..39313)
                     /locus_tag="IYA83_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ8586349.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39714..41090)
                     /locus_tag="IYA83_000898"
     CDS             complement(39714..41090)
                     /locus_tag="IYA83_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ8586350.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41218..42336)
                     /locus_tag="IYA83_000899"
     CDS             complement(41218..42336)
                     /locus_tag="IYA83_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ8586351.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42478..42912
                     /locus_tag="IYA83_000900"
     CDS             42478..42912
                     /locus_tag="IYA83_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586352.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42990..43523)
                     /locus_tag="IYA83_000901"
     CDS             complement(42990..43523)
                     /locus_tag="IYA83_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ8586353.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43617..44795)
                     /gene="mutY"
                     /locus_tag="IYA83_000902"
     CDS             complement(43617..44795)
                     /gene="mutY"
                     /locus_tag="IYA83_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ8586354.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44788..45585)
                     /gene="recX"
                     /locus_tag="IYA83_000903"
     CDS             complement(44788..45585)
                     /gene="recX"
                     /locus_tag="IYA83_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ8586355.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45692..47074
                     /gene="rlmD"
                     /locus_tag="IYA83_000904"
     CDS             45692..47074
                     /gene="rlmD"
                     /locus_tag="IYA83_000904"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ8586356.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47248..47826)
                     /locus_tag="IYA83_000905"
     CDS             complement(47248..47826)
                     /locus_tag="IYA83_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ8586357.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48148..48618
                     /locus_tag="IYA83_000906"
     CDS             48148..48618
                     /locus_tag="IYA83_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ8586358.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT        13849 a        10352 c         8442 g        16035 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
    10561 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
    10621 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
    10681 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
    10741 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
    10801 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
    10861 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
    10921 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
    10981 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
    11041 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
    11101 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
    11161 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
    11221 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
    11281 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
    11341 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
    11401 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
    11461 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
    11521 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
    11581 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
    11641 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
    11701 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
    11761 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
    11821 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
    11881 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
    11941 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
    12001 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
    12061 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
    12121 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
    12181 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
    12241 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
    12301 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
    12361 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
    12421 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
    12481 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
    12541 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
    12601 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    12661 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    12721 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    12781 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    12841 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    12901 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    12961 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    13021 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    13081 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    13141 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    13201 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    13261 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    13321 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    13381 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    13441 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    13501 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    13561 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    13621 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    13681 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    13741 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    13801 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    13861 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    13921 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    13981 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    14041 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    14101 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    14161 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    14221 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    14281 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    14341 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    14401 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    14461 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    14521 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    14581 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    14641 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    14701 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    14761 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    14821 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    14881 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    14941 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    15001 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    15061 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    15121 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    15181 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    15241 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    15301 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    15361 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    15421 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    15481 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    15541 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    15601 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    15661 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    15721 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    15781 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    15841 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    15901 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    15961 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    16021 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    16081 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    16141 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    16201 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    16261 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    16321 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    16381 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    16441 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    16501 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    16561 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    16621 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    16681 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    16741 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    16801 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    16861 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    16921 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    16981 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    17041 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    17101 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    17161 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    17221 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    17281 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttggatct cattataatg
    17341 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    17401 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    17461 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    17521 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    17581 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    17641 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    17701 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    17761 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    17821 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    17881 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    17941 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    18001 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    18061 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    18121 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    18181 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    18241 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    18301 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    18361 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    18421 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    18481 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    18541 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    18601 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    18661 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    18721 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    18781 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    18841 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    18901 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    18961 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    19021 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    19081 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    19141 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    19201 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    19261 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    19321 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    19381 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    19441 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    19501 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    19561 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    19621 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    19681 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    19741 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    19801 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    19861 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    19921 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    19981 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    20041 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    20101 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    20161 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    20221 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    20281 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    20341 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    20401 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    20461 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    20521 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    20581 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    20641 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    20701 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    20761 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    20821 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    20881 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    20941 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    21001 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    21061 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    21121 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    21181 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    21241 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    21301 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    21361 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    21421 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    21481 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    21541 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    21601 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    21661 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    21721 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    21781 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    21841 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    21901 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    21961 ttcagatatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    22021 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    22081 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    22141 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    22201 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    22261 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    22321 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    22381 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    22441 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    22501 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    22561 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    22621 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    22681 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    22741 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    22801 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    22861 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    22921 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    22981 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    23041 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    23101 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    23161 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    23221 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    23281 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    23341 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    23401 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    23461 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    23521 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    23581 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    23641 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    23701 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    23761 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    23821 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    23881 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    23941 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    24001 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    24061 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    24121 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    24181 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    24241 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    24301 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    24361 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    24421 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    24481 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    24541 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    24601 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    24661 tcggcgcatt catcactgca accattttcc cgcttccagc tggggtagct tctgtcatat
    24721 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    24781 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    24841 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    24901 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    24961 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    25021 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    25081 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    25141 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    25201 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    25261 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    25321 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    25381 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    25441 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    25501 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    25561 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    25621 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    25681 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    25741 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    25801 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    25861 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    25921 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    25981 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    26041 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    26101 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    26161 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    26221 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    26281 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    26341 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    26401 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    26461 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    26521 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    26581 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    26641 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    26701 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    26761 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    26821 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    26881 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    26941 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    27001 actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct cacatatttt
    27061 gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt gtccattatt
    27121 tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg ttaaaagaaa
    27181 aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga gatggtaagg
    27241 tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat tttcatcaga
    27301 ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat tttgatttcg
    27361 tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc caacattacc
    27421 gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta catattacca
    27481 ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct tttcgtctct
    27541 ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg ccttggttgt
    27601 ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa atgacttttg
    27661 aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag ctactgactt
    27721 acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa gccggtatgc
    27781 ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa caaaagcagt
    27841 ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag agtcagaaaa
    27901 agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct ggtgacgttt
    27961 ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt caattgtcaa
    28021 aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta gaaaaagaac
    28081 agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat tagttgcttc
    28141 tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt tattggatta
    28201 agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc tgatgagtat
    28261 ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta cacttctcta
    28321 cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg accatagcct
    28381 ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct ccttttctta
    28441 tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct aaaaaaactc
    28501 gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt gatcaaagct
    28561 tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt caatgcttgg
    28621 gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat ctttaaatct
    28681 ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat acgaggagaa
    28741 taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat cactttcttt
    28801 aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt catcgtacct
    28861 gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga accatgcaat
    28921 gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac ggcttcttct
    28981 ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc ggataccggt
    29041 gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc tgccaggctt
    29101 ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga aaaaatcccc
    29161 gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt aaagagaata
    29221 ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc tgttactaaa
    29281 ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc ttctagtaga
    29341 tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt cattttttcg
    29401 acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa caaggatttt
    29461 ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt tgattcgttc
    29521 aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa ccatgtcttg
    29581 ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa gtttagcttc
    29641 taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg ctggtgcaaa
    29701 gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc ggttcaaata
    29761 agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat aagtaagccc
    29821 ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat aaagtgctac
    29881 atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca gttctgagat
    29941 caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc tggcgttagg
    30001 ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt tctcataaga
    30061 aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg actcccttaa
    30121 tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt cgataccagt
    30181 aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa taacacgttc
    30241 acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt attttacatg
    30301 ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat acatcgtatg
    30361 gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa gtatccctgc
    30421 tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa ccaaataagc
    30481 ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg gtacacttgg
    30541 cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg taaaaatata
    30601 gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg tagcaactcc
    30661 gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt tcgcctccaa
    30721 actagtcctt gacctgattt acttttttta atttgctaga gactttttca ttcagtacac
    30781 tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct tttgctgttt
    30841 gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg atcagtcgga
    30901 taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac cagccattat
    30961 acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct aaaacaacga
    31021 tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa tacgcacctt
    31081 ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga tcttctttta
    31141 caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac caaataaatg
    31201 tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta cgttcgaaaa
    31261 acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat tctctgaact
    31321 tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg gaaatagctt
    31381 gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt gttggcgtga
    31441 tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata ggagaaagct
    31501 ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca tgatagacaa
    31561 ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt ccgtatgtat
    31621 tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata ccgacttccg
    31681 tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca agcatctttt
    31741 cactttcaaa atataataat accttcacct tacgtccccc ttcacttata tagtataaca
    31801 tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta ctgacaaaaa
    31861 cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca caccagcttt
    31921 tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc catcatatcg
    31981 cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa gcgtttcatt
    32041 aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag acccatacct
    32101 actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc tgcccatttt
    32161 ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga tgggttatat
    32221 ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt gtattgaatc
    32281 aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc tgctaagact
    32341 gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac ttccttgcct
    32401 tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg gaattctttg
    32461 agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc acgaagcaat
    32521 gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat acgcaatgca
    32581 cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag ttctttatct
    32641 ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc taatacagct
    32701 ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat atacaggaat
    32761 tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac tagatctttt
    32821 ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac agtttctgca
    32881 ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc tttttcaaat
    32941 tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc gttgacagcc
    33001 aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat tgctggaatt
    33061 tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc tgttacaaac
    33121 gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc atgtgcaaca
    33181 acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa taagtgcgct
    33241 aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata agcattatct
    33301 tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc tgattcagca
    33361 tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg atctgaaaga
    33421 accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg cgctgctttt
    33481 tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa acgagcttct
    33541 tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg aactagcagg
    33601 taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat ttcaaccatt
    33661 attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca tcagcttcgt
    33721 cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa gacataacgc
    33781 ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca tcagagttaa
    33841 atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca gtttctgcta
    33901 ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt gttgttttta
    33961 tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc ctttcatctg
    34021 taaagattaa catttttttt cgttagatac aacagttttc attcaatttc agataaaagt
    34081 attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac aaacgtgttt
    34141 atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc ctttgaccct
    34201 ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta taataagagt
    34261 caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat ctcttaaaaa
    34321 actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat gtacccattt
    34381 ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat gaggcaacga
    34441 ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt cagagctgtt
    34501 atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa aatgatccat
    34561 ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg caacagcaag
    34621 gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc aatggtggtc
    34681 agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat atcacagaac
    34741 aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc aaacgagtca
    34801 ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa ccaggtgttg
    34861 gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat gtaccgcaaa
    34921 aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa ggtactggta
    34981 tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt caagcagaaa
    35041 atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct gctggtgatg
    35101 gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa ttgcaaatgg
    35161 ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct ttagaacgtc
    35221 gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt attttgaaag
    35281 gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag gccatcgaag
    35341 cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat aaagccatcg
    35401 atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt gatcctaaaa
    35461 caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct cgagaagaag
    35521 atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa atgaaagaac
    35581 gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa aaaatcgttg
    35641 aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca caactgaaaa
    35701 acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta gatcgagtag
    35761 cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca atcggctcct
    35821 tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa cttgcctacg
    35881 aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat atggaaaaac
    35941 atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa gaagctggtc
    36001 aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat gaagtagaaa
    36061 aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt cgattgacag
    36121 atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca agtaatgccg
    36181 gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt gttactcgtt
    36241 ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt tttgacggga
    36301 tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca ctaatgctag
    36361 acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct acgaatgtca
    36421 aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct cttcgacgca
    36481 cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac ccttctatcc
    36541 atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct aaaccagaac
    36601 gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat agcgtgagac
    36661 ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa tactgttcat
    36721 tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata aaagaggtga
    36781 gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta aaccagcttg
    36841 aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact attgtctaca
    36901 ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa atccagccga
    36961 aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc gtttaccacg
    37021 aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt ccaaaacatg
    37081 aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa agagcgtggg
    37141 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc gaaagcagaa
    37201 gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    37261 ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt ctagcttttt
    37321 gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg cgtgtcttta
    37381 tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat ttgctcgttt
    37441 ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc ttttttctgt
    37501 ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct ttctttaatc
    37561 atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga ttttaatata
    37621 gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga gaggctgttt
    37681 ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt ctttacatca
    37741 aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca aattggttct
    37801 cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga cttgaactca
    37861 tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc attggagaca
    37921 tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg aattgtagct
    37981 gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc tgaatcgctc
    38041 cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg acattttttg
    38101 cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa gcaactttta
    38161 gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat acaccgatga
    38221 tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat tgtgtgacat
    38281 tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct cgttcgaatg
    38341 ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc atatttgctt
    38401 ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc tgatcatctt
    38461 gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa aaagtctgta
    38521 caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca cgagcaattt
    38581 tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct aagacttgtt
    38641 catataagga attttcagtt agaggatgat tttcgggaag cagtccatct tcatccaatt
    38701 ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga ttatggtaga
    38761 tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc ggataagatt
    38821 cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga ccatcacgat
    38881 ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact tggaataatt
    38941 tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca ctatccacgg
    39001 ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta tccaagatat
    39061 ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa atcccacgtt
    39121 gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg ccttttacgg
    39181 tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc gttgttttcc
    39241 cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt tgctttaatt
    39301 ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt ttctctaggc
    39361 acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac aaaagccgtg
    39421 tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc aataagccca
    39481 cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact taatgcagga
    39541 tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat ggattcagcg
    39601 aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga ctaaaagaaa
    39661 agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc caattatctt
    39721 cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc aggttccgct
    39781 tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga accttccatt
    39841 tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc ttcatccgga
    39901 atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact catatgcaga
    39961 tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat gatatattcg
    40021 tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc gatttctccg
    40081 acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc atattcatct
    40141 aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc atcaataaag
    40201 accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa attatcaaag
    40261 ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac tactttatct
    40321 ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc attcacattt
    40381 tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac catgacttct
    40441 cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc ttcattattc
    40501 aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt tgaatcttca
    40561 tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga aagcagccag
    40621 acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa ctttgcgact
    40681 tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac aatcgataca
    40741 taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt gccgccacct
    40801 aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga caaaatgttg
    40861 accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg caatattttt
    40921 aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg attcttattg
    40981 atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt caaaacgatc
    41041 aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat aatcgcattc
    41101 tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact atagccacgt
    41161 taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta aaactaccta
    41221 cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga cgattttgat
    41281 tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga agataccaag
    41341 caatccggca atatttccag ctactagaag aacgataatg atcgttaatg gatggatttt
    41401 cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt ggacgattag
    41461 cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta ctagaaatgc
    41521 aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc ccgcaatgac
    41581 cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg tgaaggtacc
    41641 aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt tattcagttg
    41701 ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt ggatgtttgg
    41761 cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg taatgattac
    41821 tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata ctccactaag
    41881 gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt tattgatatc
    41941 tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta cctgtttgat
    42001 gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg ggatgacact
    42061 aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga cagcccatat
    42121 tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat agtaaagaaa
    42181 gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg gttcgaaaat
    42241 aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt caagcgacca
    42301 aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt tctttcttac
    42361 tagtatatat tatattcgtt aaaaattctt ttattctttt tttatttttg aaaacttgcc
    42421 atacttttta tttgttttat tttccagtat actatacgaa aggaaggtgg ctagaccatg
    42481 aaagttgtac aaacaaaaga tacaatgagc aatatttact tagatgctgt acggatcagg
    42541 catcaagttt ttgtagtcga acagggtgtg cctttgtctc gagaaataga caaagatgaa
    42601 gcccattgta tccacttcgt cctgtactca gataaaaaag aaccgcaagg aactgtacgt
    42661 cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga tggcgattct ttctgaatac
    42721 agacaccaag ggttaggaaa aatattgatt gaagaagcag aaaactttgc taagaatcaa
    42781 ggctataata cgattttatt aggggcacag tccacagctg aaacttttta cgaaaaacta
    42841 ggatatacag cttacggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa
    42901 aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc
    42961 tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta
    43021 taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc
    43081 agaatcttca cattttcttt taggatgaag tcaatatcgt tcgggtaatt catcattttg
    43141 ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg
    43201 atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct
    43261 agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat
    43321 tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca
    43381 gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg
    43441 gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac
    43501 tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt
    43561 ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag
    43621 aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta
    43681 ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta
    43741 aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga
    43801 tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc
    43861 ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct
    43921 ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt
    43981 ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct
    44041 ccagaatgat tttgaagagc tgcactgata taataaacgt cttttggctt tgcttttttt
    44101 gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc
    44161 ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt
    44221 gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag
    44281 attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact
    44341 cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa atgcgatact tgcaatcgcg
    44401 cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt
    44461 tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag
    44521 taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct
    44581 tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg
    44641 gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa
    44701 ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt
    44761 tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa
    44821 tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt
    44881 tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa
    44941 ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagctatcg cttgttggat
    45001 cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt
    45061 ttggcttttt ccgtgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac
    45121 ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt
    45181 ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg
    45241 cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac
    45301 gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt
    45361 ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc
    45421 gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg
    45481 atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg
    45541 atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga
    45601 acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata
    45661 aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca
    45721 aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa
    45781 acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac
    45841 gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga
    45901 tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt
    45961 agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt
    46021 caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg
    46081 gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga
    46141 agctggttta tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca
    46201 agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc
    46261 acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg
    46321 tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa
    46381 aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa
    46441 tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa
    46501 agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt
    46561 tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt
    46621 ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt
    46681 agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc
    46741 aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga
    46801 gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc
    46861 accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa
    46921 acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa
    46981 aaagttcaag attgaatatt tacagtccgt cgacatgttt cctcagactg cacgttgtga
    47041 agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc
    47101 cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaatactg
    47161 tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat
    47221 agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa
    47281 tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc
    47341 ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat
    47401 ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt
    47461 ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt
    47521 taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg
    47581 ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc
    47641 ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc
    47701 tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg
    47761 aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt
    47821 tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca
    47881 caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca
    47941 gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaatc aaatcatatg
    48001 atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatta
    48061 tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa
    48121 ctaatattat agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt
    48181 tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga
    48241 aaatattact attctttgca acttccacca tttgcaccac ctagctggat atttgggccg
    48301 atgtggacct tactgtatat attgatgggt atttctcttt atttgttact taatcacaac
    48361 aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat ttttaatttt
    48421 atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca
    48481 cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctgcatca tcgcctggca
    48541 atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt gaattattca
    48601 atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag
    48661 tgccacctaa gttagatt
//
LOCUS       DACIFS010000016        45107 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.016, whole genome shotgun sequence.
ACCESSION   DACIFS010000016 DACIFS010000000
VERSION     DACIFS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.016"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(474..836)
                     /locus_tag="IYA83_000907"
     CDS             complement(474..836)
                     /locus_tag="IYA83_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586359.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(910..1290)
                     /locus_tag="IYA83_000908"
     CDS             complement(910..1290)
                     /locus_tag="IYA83_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ8586360.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1372..2547
                     /locus_tag="IYA83_000909"
     CDS             1372..2547
                     /locus_tag="IYA83_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ8586361.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2624..3208)
                     /locus_tag="IYA83_000910"
     CDS             complement(2624..3208)
                     /locus_tag="IYA83_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8586362.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3409..4407
                     /locus_tag="IYA83_000911"
     CDS             3409..4407
                     /locus_tag="IYA83_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ8586363.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4519..5028
                     /locus_tag="IYA83_000912"
     CDS             4519..5028
                     /locus_tag="IYA83_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ8586364.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPSILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5058..5717)
                     /locus_tag="IYA83_000913"
     CDS             complement(5058..5717)
                     /locus_tag="IYA83_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ8586365.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5719..6483)
                     /locus_tag="IYA83_000914"
     CDS             complement(5719..6483)
                     /locus_tag="IYA83_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ8586366.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6491..7168)
                     /locus_tag="IYA83_000915"
     CDS             complement(6491..7168)
                     /locus_tag="IYA83_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ8586367.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7205..8590)
                     /locus_tag="IYA83_000916"
     CDS             complement(7205..8590)
                     /locus_tag="IYA83_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ8586368.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8800..8884)
                     /gene="ffs"
                     /locus_tag="IYA83_000917"
     ncRNA           complement(8800..8884)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IYA83_000917"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8907..9422)
                     /gene="tadA"
                     /locus_tag="IYA83_000918"
     CDS             complement(8907..9422)
                     /gene="tadA"
                     /locus_tag="IYA83_000918"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ8586369.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9529..10386
                     /locus_tag="IYA83_000919"
     CDS             9529..10386
                     /locus_tag="IYA83_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ8586370.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10467..10802)
                     /locus_tag="IYA83_000920"
     CDS             complement(10467..10802)
                     /locus_tag="IYA83_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586371.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10869..12239)
                     /locus_tag="IYA83_000921"
     CDS             complement(10869..12239)
                     /locus_tag="IYA83_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ8586372.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12317..12853)
                     /locus_tag="IYA83_000922"
     CDS             complement(12317..12853)
                     /locus_tag="IYA83_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8586373.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13162..14643
                     /locus_tag="IYA83_000923"
     CDS             13162..14643
                     /locus_tag="IYA83_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ8586374.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14746..15453)
                     /locus_tag="IYA83_000924"
     CDS             complement(14746..15453)
                     /locus_tag="IYA83_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ8586375.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15532..15870)
                     /locus_tag="IYA83_000925"
     CDS             complement(15532..15870)
                     /locus_tag="IYA83_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ8586376.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15981..16943)
                     /locus_tag="IYA83_000926"
     CDS             complement(15981..16943)
                     /locus_tag="IYA83_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ8586377.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16958..18289)
                     /locus_tag="IYA83_000927"
     CDS             complement(16958..18289)
                     /locus_tag="IYA83_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ8586378.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18297..18977)
                     /locus_tag="IYA83_000928"
     CDS             complement(18297..18977)
                     /locus_tag="IYA83_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ8586379.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19196..19675)
                     /locus_tag="IYA83_000929"
     CDS             complement(19196..19675)
                     /locus_tag="IYA83_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ8586380.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19802..21481)
                     /locus_tag="IYA83_000930"
     CDS             complement(19802..21481)
                     /locus_tag="IYA83_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ8586381.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22029..23531
                     /locus_tag="IYA83_000931"
     CDS             22029..23531
                     /locus_tag="IYA83_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586382.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23544..24206
                     /locus_tag="IYA83_000932"
     CDS             23544..24206
                     /locus_tag="IYA83_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586383.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24307..24510)
                     /locus_tag="IYA83_000933"
     CDS             complement(24307..24510)
                     /locus_tag="IYA83_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ8586384.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24621..25805)
                     /gene="iadA"
                     /locus_tag="IYA83_000934"
     CDS             complement(24621..25805)
                     /gene="iadA"
                     /locus_tag="IYA83_000934"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ8586385.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25962..27701)
                     /gene="yfcC"
                     /locus_tag="IYA83_000935"
     CDS             complement(25962..27701)
                     /gene="yfcC"
                     /locus_tag="IYA83_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ8586386.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28473..29087)
                     /locus_tag="IYA83_000936"
     CDS             complement(28473..29087)
                     /locus_tag="IYA83_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8586387.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29693..30070)
                     /locus_tag="IYA83_000937"
     CDS             complement(29693..30070)
                     /locus_tag="IYA83_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586388.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30370..31251)
                     /gene="galU"
                     /locus_tag="IYA83_000938"
     CDS             complement(30370..31251)
                     /gene="galU"
                     /locus_tag="IYA83_000938"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ8586389.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(31294..32313)
                     /locus_tag="IYA83_000939"
     CDS             complement(31294..32313)
                     /locus_tag="IYA83_000939"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ8586390.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(32946..33950)
                     /locus_tag="IYA83_000940"
     CDS             complement(32946..33950)
                     /locus_tag="IYA83_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8586391.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(33963..35012)
                     /locus_tag="IYA83_000941"
     CDS             complement(33963..35012)
                     /locus_tag="IYA83_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8586392.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(35029..35850)
                     /locus_tag="IYA83_000942"
     CDS             complement(35029..35850)
                     /locus_tag="IYA83_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ8586393.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(35837..36616)
                     /locus_tag="IYA83_000943"
     CDS             complement(35837..36616)
                     /locus_tag="IYA83_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586394.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(36633..37103)
                     /locus_tag="IYA83_000944"
     CDS             complement(36633..37103)
                     /locus_tag="IYA83_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586395.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(37115..37534)
                     /locus_tag="IYA83_000945"
     CDS             complement(37115..37534)
                     /locus_tag="IYA83_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ8586396.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(37615..40383)
                     /locus_tag="IYA83_000946"
     CDS             complement(37615..40383)
                     /locus_tag="IYA83_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8586397.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            40583..40807
                     /locus_tag="IYA83_000947"
     CDS             40583..40807
                     /locus_tag="IYA83_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586398.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            41586..43301
                     /locus_tag="IYA83_000948"
     CDS             41586..43301
                     /locus_tag="IYA83_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586399.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            43304..45067
                     /locus_tag="IYA83_000949"
     CDS             43304..45067
                     /locus_tag="IYA83_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586400.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT        13386 a         9321 c         7612 g        14788 t
ORIGIN      
        1 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
       61 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      121 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
      181 ataactattt tttaaatttt ttagaaatgt gatataacat ccacttcaga atcaacgctg
      241 ttaaatttat catgtatttc ttccttggtc aactataaat taagcattac tttgtgaaat
      301 gatttttcga tcaattatta ttattcgtta tacaaccgtt ttcaacatat tttgaaaata
      361 aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg accaaacaat
      421 aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag
      481 aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc
      541 caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac
      601 aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta
      661 acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct
      721 tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt
      781 ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt
      841 tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa
      901 atacactttt tatttcttat tttctttccg ttctagttca gataatgctt cttctatcca
      961 aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat tcgtgaaata
     1021 cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta aagataaact
     1081 gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct gtccgacttt
     1141 taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat gaataagtcc
     1201 ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg tcacttttcc
     1261 ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat ttatcattat
     1321 actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa
     1381 tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc aattgctgag
     1441 acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt cttgtctccc
     1501 ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg ttatgccact
     1561 ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca tgctgtggag
     1621 ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta
     1681 caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta tcctccattt
     1741 tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat
     1801 aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa
     1861 gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa agaagaattg
     1921 aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga cgagatccat
     1981 caagatttaa ctttattcga tcatacattt acttccatgc taacgattga tcctaattta
     2041 gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc tgcaatcaaa
     2101 aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa acatttagtg
     2161 atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc ggcttatgag
     2221 acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt
     2281 aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga aggaacttac
     2341 ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga gaacactctt
     2401 gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc cggtcatggg
     2461 catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact taatcgaatc
     2521 aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga cgagacctta
     2581 ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct gaaatttcaa
     2641 ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt acatttgcga
     2701 tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt cggaaatcta
     2761 gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat ccagcacctt
     2821 ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag aattggcttc
     2881 cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg aacagttcac
     2941 gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt cctgttccag
     3001 ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc acgccatcat
     3061 aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct agttcaggga
     3121 ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt cctttttcac
     3181 tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc atacttcata
     3241 ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt cttttatttt
     3301 tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac atttttgaga
     3361 agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat ggaaatctat
     3421 gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta cgcaggaatg
     3481 cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca attagcaact
     3541 ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa agcaagtgaa
     3601 ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca tttaaaagaa
     3661 gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa taaaggcatc
     3721 cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc aagaaaattg
     3781 aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt gaatgatttg
     3841 atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc tatcgattgg
     3901 acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt
     3961 cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt gcttactccc
     4021 tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg gttaaaaaaa
     4081 gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa ttatggattc
     4141 tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa tgccattact
     4201 gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga catctgtacc
     4261 tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac tgcagtgaac
     4321 aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag cacaagctta
     4381 tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc aagttaaacc
     4441 cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa
     4501 taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa
     4561 ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat gtttttacaa
     4621 aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa cagcgtgcta
     4681 acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat attagcagaa
     4741 aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg cctttacggt
     4801 atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat
     4861 tatggttata tcgacaaggt gaaacctagt attttaaaag aactaaacag ccacgatggc
     4921 gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc tgctgccagt
     4981 cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa
     5041 agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtttcaa gctatttttt
     5101 cctaagaaaa gaaaaacagc aaaaaataat tctaataata caaaaaataa gataaagcta
     5161 tgcatgaaaa attcttctgg aagtacattg atgattggat cagtaacggg atcgaataac
     5221 cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc aaatcctatc
     5281 atcatgaaaa aaccgaaaac aactggcagt aacatgccga tttgaaacgg acgaatcaac
     5341 cgccaaagcc gatcattctt ttgcaaatat ttgataaaca aaatactcgg gatcaaagtg
     5401 atgaaaaaca cagcataatc cactaaaaac aaaattttca cttcataaaa gtgatgggct
     5461 ccgctagcag aaactgggaa atctggcaag ctgaggatgg ttttgaaggg attattgaga
     5521 taattcatta ggtgatcaaa atttttcagt aacgtctctt gatctaaaga agtataatcc
     5581 aaaatttgca aatgtccgat atcaaatacg tatagtggtc gaaagttgat cgtcacacca
     5641 actgccagac tgatcagtgt cagaaatagg ctgaacaatc cagcatattc catccactgc
     5701 catctcgaat ttttcatatt agaagctcca ttcatccagt gattgtttca cataggtagc
     5761 tgggataggc aacccaggta catcttcttc ttttgtaaaa cctgataaaa cgagcaaagt
     5821 atctatatta ttgcgtatcc cagcttggat atctgtttca tagttatctc caaccatgat
     5881 tacttcttct ttttgcagcc ctaatacttc aacggcttta tccataatga tggcttctgg
     5941 ttttccgatg tagatcggcg gcgtttgggt tgctgtctct acaaaagaga tgactgaccc
     6001 tgctccagga agaaggccgc gttcagtagg tatattctta tctgggttcg ttccaatgaa
     6061 agtagcacct ttttgaatag caagtgttgc tttaactact ttttcatagg tcagataatt
     6121 atctaagcct accactacat agtcaggagc ctcttcttcc catgtaaatc cggcagctaa
     6181 aatcaagtca atcaatcctg cttctccgat aacatagact tttttccctt ttccatctgc
     6241 tttcataaaa tcgattgttg ccaaagtagc tgtgtagact gtctctggag ctacatgaat
     6301 gtcaaactca tctgctagac gattagctac tgtttctggt gacttagtcg tgttattggt
     6361 aacaaacaag aaaggtaatt ttcttttttg caattcttcc acaaatcgtt taccggccgg
     6421 tatgggttgt gtcccacgat aaatcgttcc gtctaaatca attaaatatc ctttatagtc
     6481 cattcatcca ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt ttttctactt
     6541 gtttaggttg ttcttgtttt tttacttttt cagaggtttt tgctgactct gtttttcgat
     6601 ttttaataac cggttttttg cgattagatg cgactggtcc ttttttctca tcgatatacg
     6661 cacgattatg gttttttttc ttttttcggc gctgagttgt cttttctttt ttaccgccga
     6721 ttctctcgat gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga tagtcttcca
     6781 aggaatcgat tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa aatcctttta
     6841 ggcgtagttg ttcataaccc caatctccta cgatataatc atatcgggct agtacatcac
     6901 tgtaacgttc tccaagcttt tctgcattaa atccttcacg ataatcggac acaagccgat
     6961 aatgtctttc acctatcaaa aaattactgt catctaacag tgtcactagc tcgcctttga
     7021 ctttttctgc tttaggctcc tcaacgattt cttccaaagc agctgtcagt tcttcggtga
     7081 ctgttgtttc ctgttcagct gatgattttt tcttagcaat ttctgtttta tgctcaacgt
     7141 tctgctgttc attttgtttt gcagacatgc gttcaccacc tatctttttt attatacctt
     7201 gtttttattt gattgggtag tgagctttca agtaatctcc taccacactt cggataaatt
     7261 cagggaacaa aaattcattt tttccagcaa tctcaatcgt tggaaaaaat ggaacaaaca
     7321 tcaaatgatc tacggtagta aaagtataat ttttattcag atcgattggc ttgttcttcc
     7381 aaaagacttg atggttaata gaatcatatg tgattccatt atagaccatc tgcccaaaaa
     7441 tcttaccgcg aaatcccatt cccttcatag gatagttacg taaaaagtta cgattttttt
     7501 ctatttctaa aactaagcgg atcagatcat ttccacccaa tgtgacattt aacaagtgca
     7561 tcggatgcgg caacgcagtg tgaagctgat cctgattgac ccgccctttt ggaatttcag
     7621 ttaaaaacag accactgttt aatattgctg cttccgtctt tcctcgttct ttcaccgcct
     7681 caaaggctac ttggataaac gaatgctcat tgaagatatc tagactcaga gaaaatggca
     7741 aatctgctac ttctttttct tttaataatt catgcccacg ttctagataa ctatttattt
     7801 cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg gatggctttg gctgaatgct
     7861 taaggatttt tttctcttca tcgatggtta aatggacttc accgacatac tgcccgtact
     7921 ttccagcggc tgctatttgg acatcattga ttacgtgacc ttctctgaat aaatgatggg
     7981 tgtgcgatcc taaaataaca tcaattgctg gtatttcttt tgcaatttgg aaatcatcct
     8041 gaatcccgag atgactcatc aaaactaaaa catccacttg tgaacgtaaa tcttcgacta
     8101 attctggcaa aatatcataa ggattccgta tgtcccagcc attcggatta taagtaagcg
     8161 gaaatggagc agtaaatgca attagtccga ttttcgtctg gtgtttcgtt gtaatgattt
     8221 tatatggttt cgtccatgcc ggtggctgga gcgtattttt atcgaataag ttatccagga
     8281 tgatatcaaa ctcagcatga tcgtataaat gattcaactc ttctttggaa tttcctacgc
     8341 cttcattgtt tcctatcgtc gctgcatcat aaccaacttc attcatcagc aagatatttt
     8401 cctgtccatt cgttgcttct gacaatggat gccaacgatc cacaaaatca cctagatcta
     8461 ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg tttacgttga tccaaaaacc
     8521 ttctgatcct tggccaattt tctaaatgag aatgcagatc attcgtatgt aaaatcacta
     8581 ttttttccat gtaccctctc gcctctcttt atctttatca taccataggc agctttttct
     8641 tccattgaaa caagctctat ttcagagttt ttcctgcctt gaagcaaatt gtataagtac
     8701 aatattcgat aaatcagtct ggctaaaaaa tcaggaacat tcatttttac agtactataa
     8761 aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat tataatcaga cacatggcgg
     8821 aacctgctta gtgctgcttc cttccagacc tgacacgctt cacaagccca ccattgtcct
     8881 agcctttcgg caaagaatag tataccttat ttttcattcg ttgaccagtc tttttttaat
     8941 ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc acattcgtct
     9001 tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc caaaagattc
     9061 atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac ttctgctaca
     9121 cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac aaaaagtgca
     9181 gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc aagcatttct
     9241 gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc aatcactttt
     9301 ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc tttctttgct
     9361 tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt tgtatttacc
     9421 aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat aaaaaggctg
     9481 taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat gaaaatggat
     9541 aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc agaagcggat
     9601 atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc attatctcaa
     9661 caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa aaaacaccca
     9721 tggtatcact atctttttaa agatgcttct tgtgttcaag aaaaaagctt tcgtttgatc
     9781 cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga tactattttg
     9841 gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc gctcttagtc
     9901 gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt tctatcttta
     9961 gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc acctggcacc
    10021 gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca aaacaatctt
    10081 tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta tacaaaagat
    10141 actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca aaatgaaatg
    10201 gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc tgcaaaaaca
    10261 ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga acagtctggt
    10321 ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct tcatgaacat
    10381 aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct tatttcgaat
    10441 gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg cattagccgg
    10501 tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat tttctttttt
    10561 cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc tgaaagacca
    10621 tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa tttttttact
    10681 ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac aacgactgct
    10741 gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt tgatgttttc
    10801 ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa ataaacgaac
    10861 taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt tccttttact
    10921 tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc ttgattccca
    10981 tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt gatcggcaag
    11041 caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg ttcaaatcga
    11101 ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc atcgagagaa
    11161 ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct tccagtagct
    11221 tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt tgtcatttgc
    11281 actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc atgaaaagtc
    11341 agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg tattgttttt
    11401 tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg agtcaaagga
    11461 atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc ttcattttct
    11521 tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag catttttatt
    11581 gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc ttctgtccac
    11641 tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg agcatcaacc
    11701 acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc atgatttgtc
    11761 actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt cgcacgtttc
    11821 ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc taattgggtt
    11881 gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt tgctgcagca
    11941 gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc ttgatggagg
    12001 aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc gggttgtaat
    12061 tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg attccaccga
    12121 aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc ttcttcgcct
    12181 aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc ctccttcatt
    12241 ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg gctgatctac
    12301 tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag tattcatccg
    12361 tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga gaagaatcat
    12421 ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt tcttctttga
    12481 tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa tccatgtcta
    12541 aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa tctttcaata
    12601 agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt tcaaaataaa
    12661 cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa tgtgtataaa
    12721 tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt gacttcattc
    12781 catattcttc cacgtatacc atacttttga acagcaaata ttctttacta taatttgtta
    12841 ctctccccat cattcttacc tccgtctctt ttcatctacc cataaataaa taacataaat
    12901 aattctaaaa atattattga taattatcaa aacatattta gttatatcca ttaaaaatca
    12961 atttttccgc cttaaaaaca agaatatctt gacaaaatac cctaggaaat aaacaataga
    13021 ataaacaagg taacaaaaaa attaaataac gttatataaa aaagaaggcg acactctgtg
    13081 agaataacac agactgaaat gactttccac tcacagtttt tgtctaacta tatacaaaaa
    13141 cagaataaaa aggaggtatt tatgctaatt gaagcaagaa aaaaagagtt acttgttcta
    13201 caaatggtca ttcaaaaaaa taattgttct ttacaagaat tatccaatga cttacatatt
    13261 ccaaaacgga caatcaaaga actgatccga aaaataaatg tatcactcaa gcaattttta
    13321 ttgattgatg aatttattta ttctaatcat aaaggagaga tccaaaccaa agaaaccgat
    13381 caaactaaaa agttagctgt tttttctaag ttaaaattat gctacctaaa agaatccaat
    13441 cgttttaact atttgatgtt actaatcaat tatccacaaa ctgctgtacc aaaaaaatat
    13501 ttacttgaac aactttatat atcgccctcc tatctcgaaa aactgacaaa acagctcaat
    13561 cattccttaa aaaaattcca tttacggatc ctatctgtaa aaaattgctg tactcttgaa
    13621 ggaaatgagt tatctatccg tctctatctt tacttttttc tttcagatac atttggaggt
    13681 aacgattggc cattttcctc acttgaatta aatgcactaa aaatgaacag tacagatgag
    13741 caaatagaaa aaatttcaaa acaagaaaaa aatatctact tgctgttaac tttattcata
    13801 ttacgaaaca aacaacaggc atattttcca aaaacttctt ctgaaatcac tgaattgatg
    13861 gacttattgc agcatacaca tgattattca gaatacttta cttctttttc agtgagtcgc
    13921 ccttgtgaag agacagaaac agagagagcc tactttaatt attttttcta ttgttttttt
    13981 ccaaatcaaa tctcttttcg acaaaaacag gagattggtt ttttattttc cacaagcaaa
    14041 aaccttattt gtcgacaagt cggtcaattg atgacttact taactcaaca gtttcctctc
    14101 aaccatttag aaaataaaat accaatttat ttttattttc ttgtgttggc agttgttttt
    14161 ttcgaactgt tgggtgaatc cagcagtagt ttttctacac tttgctttcc agaagccacg
    14221 tccaatcatt ctagttcttt tatacaggaa aagaaattaa gagaaaaaat cgatacaatt
    14281 ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc ttagtcaatt gatccaacac
    14341 ttactacaaa cagaaacgaa agagcagtta ctcatccatt tgcagataac caaacagttt
    14401 acaggaaatt atcatcttca aagccatttg cagcagttat ttcgcaaaga tgctgttaaa
    14461 attaccgcat attcagaaca tgctgatttg atcattacag atcagcatga gaaaatttca
    14521 gatgaaacgg agactttcta tctggataca acgcacaata cagtgttatt tggcaagtta
    14581 ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg agaacaaaac aaagaggatg
    14641 tgacaaaatt cttgtcacat cctcttattc caaataaatg gtattcaatc agctaactct
    14701 cggtattagc tcagatcaaa gcgctgattt cacaacttct ttattttaat ctcttcggtt
    14761 gccgccgaac aataagatca agcgcaacag ctgcaggaat gtcgaaagcg ctgctgcaac
    14821 ataagtcagt gctgctgcaa ataatacgtg tcttgccatc ggtacttctt cttccgtcag
    14881 tagtccgcct tcactcaaaa tttggattgc tcgccgagac gcgttaaatt cgactggtaa
    14941 tgtcaccaat tggaaaagta atgctaaaga aaaagcgaaa atcccgatat tgattaacgt
    15001 agagttccag ctaagcaata cacctaccat aatcaagggg aacgaaatca tcgaaccaaa
    15061 gttagctgct ggaacaagtg ctgcacgaat cttcaatggg acataacccg aagcatcttg
    15121 taccgcatgt ccgcattcat gagctgctac acctatcgca gcaactgaag tagactgtgc
    15181 agttgcttga gacaaactca acatcttatt acctgaattg tagttatctg tgagatctcc
    15241 ggcaatttgt tgaacaccga cattatttat ctgttgactt tgtaatatat attgtgctgc
    15301 ttgtgtacca gtcacattgt ttttgcttct cacttgatcg tattttcgga aagtactatt
    15361 gacgtacgct gacgcaattc cggaaataac taatccaaga atgaccaaaa gaaatgtcgg
    15421 atctaaaaaa ccatacattc cataaccata cattttcgtt tcactccttt tcgtttactt
    15481 tattttatca ttagtagtag gaaaagaatg tgaaaagacc ttgaatattc tttaattctt
    15541 tttcacaaat tccgatttca gtttcatcgg tccaaatcct tctaccttgc aatcgatatt
    15601 atgatcgcct tctactaaac ggatgttctt gacttttgtt ccttgtttaa tggcacctga
    15661 cgcacctttt actttcaagt ctttaatcac tgtcacgcta tcgccctcta acagacggtt
    15721 tccatttgca tctaatacca ctaatccgtc ttctatgaca gtttcttgtt cttctttgtt
    15781 ccattcgttc ccgcattctg ggcaaatcaa caagcctcga tcttcatatg tgtactctga
    15841 gccacattct gggcaatttg gtaatgtcat cttctttctc ctttaaaatc tttagtgagc
    15901 actattgtaa cggaaaaaga agatcttgaa aagatacatt tgtgattttt actacaaaag
    15961 gcacgttttc gaacgaagta tcattctgtt tttctgctct tatacgattg gcatatatct
    16021 tggatctctt tatagatttc ctctacttct gctaaatctt ttgcattcgc accacttgct
    16081 aatggatggc ctcctccatg atggcgttta gccaattcat tgatgatcgg ccccttagaa
    16141 cgtaaacgta cgcggtaata ccctgaaggc tgctgaacaa aaatccccca agccagcact
    16201 tcgtcaatca ctccaggaag tgaaacgatc gaagctgtct ctgaatcatc gattccatat
    16261 ttatcaagaa tttcttgcgg aaggattatt cttcctgcac cattttcatc cacctggata
    16321 ttttgataaa gataaccgga aagtttagcg actttcatcg gcatttgttc aatctctctg
    16381 ttcaaagcac ttgcatcaaa tggataattt ctcaatatcg aagcaatctc taatgtacgg
    16441 gacgtcgtgg agggatacaa aaagcgccct gtatctccga cgattcctgc ataaagtaac
    16501 cgtgcagcct taacgtttat ctttaaagat tcaggaaaca tcaaagcaaa ttctgcaatc
    16561 atctcactac agctacttgc ttttgtattt acccacacaa gatctccata agggtcatca
    16621 ttgggatgat ggtcgatttt gatcagtttt tctgccaact gatatctttg atcactgatt
    16681 ctgggtgaat tagctgtatc agtcacgatg actagcgctc cttggtagaa gacatcttca
    16741 acttcttgca tttctgctaa aaagcgaagc ccttcaatcg tttcacctac ttgacgtatt
    16801 tctttttctg ggaaactttc ccgtaatagt tcggctaatc ctacttgtga tcctaatgca
    16861 tcaggatcag ggcgcaaatg cctgtggata atgattttat cgtatttttt tattgtatct
    16921 actatttctt gctgtactgt catttcactc ctgctttcta agaacgttcc attacttgac
    16981 aaacaacgat tgctttggaa acgatggtgt tttcaataaa cacttcgata tccagtttag
    17041 cggaacgtct gccgatttcc aatacacgtg gacgaatgtc taattcgctt tccaattgga
    17101 tcagccgcaa ataatgtagg ttcacttgtt caatcaagat atttcgtttt tggttcatca
    17161 ccattgtttt ttgtgcgaca tcagaaatga tttcgctaag tacgccaaaa gaaatggttc
    17221 caacgccatt gaccatttgt ggtgctacta cgaatttgaa cttagggttt tccaaacgat
    17281 ttccttcacg gtcagtatca atcgtatgga tatttccaga tatctgatcc gaaatcgtat
    17341 ctgcaatctg cggttgtcgc tggacaagct gcatcgcttt cattacatct tgccgggtaa
    17401 tcaagccttc caatgtcaag tcatcggaaa cgaccggcat gacttctagc ccatcccaga
    17461 tcatttgatg gctaacagag gcaatactca ttgttttctt tacactgatt ggttctttcg
    17521 tcatcacccg atcgatgatc tggtttggcg atttccctaa aacatctttt gccgtaacaa
    17581 tccccatcaa acgatgatgg cggttgacga ctggaaaacg cgaatgctgc gtttcttctg
    17641 acaaacgctg ataatcagaa atcgtatctg tctgctgcaa ataatgagtc ttttcagctg
    17701 gcatatagat atcactgaca agcatgatat cttttttgat caattggtcg cttaacgctc
    17761 ggttgatcat ggttgcgact gtaaatgtat cataagaggt gcgtaagatc ggcatttcaa
    17821 gatcatcagc tagctttgca atctcagcag tcgtattgaa tcctcctgtg atcaatactg
    17881 cggcgccatc ttccaatgct aatttttgga cttccgcccg gttgccgacg atcattagtg
    17941 atcctggcgt aatatagcgt tccatggcat gtttagtcat cgcgccgatg acgaatttat
    18001 tcaagacctt gtctaatcct gccgccccgc ctaaaacgtc cccttcaatg atcctgacga
    18061 cctcgccaaa tgtcaaacgt tcaatatgtt tttttaattt gcgttcgata cggatggtcc
    18121 ctactcgttg aattgttgaa actagtccga cattttctgc atctttgatt gcacggtacg
    18181 ctgttccttc actgacattc aattcttttg cgatgcttct tacagaaatt cgattgccaa
    18241 caggtaaact ttcaatatat tctaaaatca aatcatgttt tgtcgccatt ttctgcctac
    18301 acttccagcg tttccgagac gctcattatt tttccttcac tatcttccag taactctaca
    18361 aaagattcgg gatcttgttc aatgacaggg aaagtattgt aatggattgg aactactttc
    18421 ttggcacgaa ttgtttgcgc agcctttgct gcatcttctg gccccatcgt atagttatcg
    18481 ccaatcggca agaaagcgac atcaatatcg aattcttctg caagcaatcc tagatcactg
    18541 aaataagcag tatcccctgc atgataaatc gtttttcctt ctgcttgaat aataattccg
    18601 ctaggctcgc ccatatactg aacgataccg tctacttcat agcttgaact atgctgtgca
    18661 tgtacgaatt ttaccacgcc gaatggaaac gcatgttttc caccgatatt cataccgaat
    18721 gaatctactc ctctgctgtt cgcataatca gcaatctcaa caatactgat aattgtggct
    18781 tgattttctt ttgcaatagg aatcatatct cctatgtggt cactatgacc atgagtaatc
    18841 agcaaccaat ctgtctttac ttcttctgca gttaagtctg tcaatggatt tcctgtaata
    18901 aacgggtcaa tcatcacgcg ttggccattt tccatttcaa ttgttaaaca ggaatgtcca
    18961 tgatacgtaa ttttcatatt ttacacctcg cgttttctct gtatcttttt tattaaaaac
    19021 gatttcatca cttgcacatc aatgaaaaaa tatagctgct aaatatattt tcattggatt
    19081 gatagtacag tatgtacaaa gcaattgtaa cagataatac aggatgcacc aagtaccaag
    19141 cagatataat ctcaagtttg aggtctctgc tctgcttagc ttctcttctc gtcccttaac
    19201 taattaaatc agcgtaaata ggatggacga tcttttctaa atcggctact tttatccgaa
    19261 ggcttctccc gatttcaccg cttgacacaa ttacttcttc tttttctttt aaagaactgt
    19321 ctgctaaaaa acaataatca ggatgtgcga gttttattcc aataggggtg tttgctccgt
    19381 ggggatatcc tgtatgcatt aatacaaaat ccattccagg aaaaccaact ttacgatctt
    19441 tcgtcacttg tctcgttttt ttgtaatcta agtgatcgtt caaaggaatc aatgcgatga
    19501 tcaccccagt tttgtttcct ttcactacga gagttttaca tatcaaagct ggatcaatcc
    19561 ctcttttttg tagttcttct gtgaaagatt cttctaccac atgactaaaa tcaagtggtt
    19621 gatagctgat cttttgttct tctaaataac gttcgacttt tgtttgactc atcattgatt
    19681 cactcccttt attctatcgt ttattataaa gcgagggatg ctgtattcaa aaattatttg
    19741 tttttcgcaa aaaaagagca agacgagcac tctatagaga ggttcatctt actctttttg
    19801 tttatttttt atttcgacgt tgagtagctt ccataatgac tggcaagatc actgggcggc
    19861 gttttgtttg ttcaaataag taacggccta attgatctcg gatgtcttgc tttaatttgc
    19921 tccactcaaa atcattgctt tctaaatgtt tctcaacgat tgtttcaatc atattgctgc
    19981 tttctttcat cagatcacgg ctagctttta cgtaaacgaa tccccgtgaa gtaatttgag
    20041 gttttgaaat gattttcttt tctcgccgac tgatagtgac aaccgctacg aaaatcccgt
    20101 cttctgacaa gatttttcga tcacgtaaaa caatgtttcc gatatcacct atgcctaatc
    20161 catcaatcat tacattttct gcacttgtag ttcctgcaac actcatacgc ttgttcttat
    20221 attccagcac gtctccgcgt ccagtaatat agatattttt gtatggcatc cctactgcat
    20281 gagccaaatc tgcatgagca gctaattcac gatactctcc ttgaactggc acaaaatatg
    20341 ttggtttcat caaattgatc attaattgca agtcattagg attggcatgg ccagaaacgc
    20401 gcatattctc agagatcaac ttcactgttc caccagctcg ataaatgata tcttccgttt
    20461 tcgccacgat ggtttccatc gcgatgcttg gcgtagtagt gatataaacc aaatctcctt
    20521 cttcaatctt gacattacgg tgtgtaccgt ttgccatttt ttgcagagac ttgatgggtt
    20581 cacccattcg gccagtttct aaaatgatca gttcttctgg atcatagttt ttcatgtctt
    20641 tcaacgtaac aagcagatct tcacttggta attgaagttt ttctaaacgc atcgctgtgc
    20701 gaataattcg ttcgaaatct tgaccagtca agacaacttt tcgttgtgct ttttctgctg
    20761 catccaatac ttgttgcact cgttgcaaat tacttgctac acaagcgacg atgatacgcc
    20821 cttcccaata tttgatcgta tcaaatactt catctgctat ttgggcttca gaagctacag
    20881 gctgaggatt ttctgcattt gccgattcac ttagtaaggc taagactcct tctttgccga
    20941 tttccgccaa tcgtgcataa tctgtttgat acattgggat agccgtttgg tcaaatttaa
    21001 aatctcccgt ataaacgata ctaccttctt ttgttttcag attgatcccc actgaatctg
    21061 gtatcgtatg agtggtacgg aaaaagctca ctactgtttc accaaagtct atttctgtat
    21121 gctcatctac cacatggaaa tccttaaatc ctttggctgc ttcattacga ctgacattca
    21181 attttgctaa ctcgatcgtc aactctgtcc cgaatactgg tacagaaatt tttgacaaca
    21241 agtaaggaag tgccccgatc gcatctgcat gtccatgtgt taaaaagaca ccagctacac
    21301 gatcaatgtt ttcttctaaa tacgtaaaat cagggattac gacgtcgatc cccaacaact
    21361 cattttcagg atattttagc ccacaatcta aaataaaaat atcctcgccc acttctgcga
    21421 catacatgtt ttttccattt tcacgaacgc cgcctaatgg gataatttta atggtactca
    21481 caaacttcac ctcttcttat gacatccaag tttaaagatg tcgattttct tctttatcta
    21541 aatatatgag ccattccaat gaagcatatg caaaatcact atttctcaac ttatggagaa
    21601 tcagcaggaa aattaccagc acatccgcgt tctgacagtt ggatgggcat tttattttgg
    21661 atatacttca ttaacaaaat ggatcttttg cgaaacgcta tagccagaat acttatataa
    21721 gtattcagct gttccccatt attatacaac aaataaacta aatcgctagt atcagcaatt
    21781 gttagaacaa ctatgcacgt aaaaatccct ctgtagcatc taggatttct tttgctattc
    21841 ctctttcatt tcttcttcgt cggtttgaaa catacttttt tcattttacc acattttttg
    21901 caataactat agtgactttt tatatataca gaacctcatg ttgatggtgt gcaaacagca
    21961 taagatttaa gctagcttta agataggttg tctattatca aatgcatcta ctaggaggga
    22021 tgcaaaatat gaattttatg aaacgtgcat ggaaaagtac aaaagtaaaa tggggacgtt
    22081 cattgttatt gtttgccgtg ttcacagcca ttcttatttt tgtgctagct ggcctcacga
    22141 ttcgaagcgc tgcagaagaa gcagcgaatc aagcacaaaa agaagttgga gcaacagtga
    22201 ctttaagtgc caatcgagaa gcttcattta aaaaacagga acaaacttct tcaagcggtt
    22261 ctaccactga agaacgtcca gatccaggca gtttttctct tacaccagtt tccttaacgg
    22321 acgcggaaaa aatagctgaa ttagataatg taaagagtta ttcctttgaa agttccgcct
    22381 cagctatggc aaaagatgga ataactgcca tcactagttc tgattcaagt gaagacagtt
    22441 cagaaactac tgatgcaggc ggtgaacctg ctggaggaat gggcgggaat ccgcaaatga
    22501 ctcaagcaga ctttcaaatc agtggtgtgt cagcaactgc acaaaatagt aatttttctg
    22561 atggaacagc aaaaatcacg gatggtgaag gaatagatga cagcgacaaa ggcacaaaca
    22621 atgtagtcat tgattctact ctagcagaaa caaatgacct ggcagtcgga gatactttca
    22681 caatcacgag tactgaagat gaagatacca cttatgaaat gacgatcaaa ggaatttatg
    22741 aatccagcga aacaagcggc agtatgggta tgaaattcaa cttcatgaat ccgtcgaata
    22801 cactttacac ttcttacact tttgcaaatg aactaaatgg gacatctgac gataacacga
    22861 tagattctgc tatttacact ctatctgatc cagataaaat ggatgagttt ctcaaagaag
    22921 ctgagaagct gatcgacaca gatacattta gcttacaatc aaatgattcc atgtatcagt
    22981 caatgttgga acctttaaac aacgtggcaa gtttttctaa aaatgttgtt ttactagtgg
    23041 ctgtagccgg tattatcata cttactctga tcattatgat taccattcgg gagcgtcggc
    23101 atgagttagg cgtcttgcta tctttaggag aatccagaag taaaatcatt ctccaattgt
    23161 ttaccgaagt ggctatttgt atgattcttg cattaggagt tgcttcgctt agcggaaacg
    23221 tcgtcgcgaa tgccgtggga cagcagctac ttgaccaaca gacagaaacc acgacacaaa
    23281 accagcaagc tcctggcggt ggacaaatgc caggtaacag acaagaacaa ggcggtcaac
    23341 gcgatggtca acaaggaacc acgaataatc catttgctgt ttctgatcaa gtcagcgaat
    23401 tggaaatcaa tgtcaaacct gcacaattgg gactattagc cggagtagca tttggaatca
    23461 gtctgttttc tgtcttttta gcttctatag ggatattaag attaaaccca agaaaaatcc
    23521 tattaaacta aaggaggaat ttgatgttac ggacagaaca attgggattt tggtatcaag
    23581 atccgcaaga tgcgctttat aaagatgtag aactgaattt cgaagctgga aaaatgtacg
    23641 ctattttagg agcaagtggt tctggaaaaa cgacttttct ttcgcttatt tctggactag
    23701 atgcaccaaa agaaggaaag gtatactatc aagatcgttc actgaacaaa atcgggttaa
    23761 gaagttatcg taaaaatgat gtctcgatta ttttccaagc ttacaacttg cttccttatt
    23821 tatcggctct agacaacgtc atagcagcaa tggaaatcac taagaccaag caagagaatt
    23881 atcgacaagt tgctttagat cgactggctc gtgtaggaat tgatgaaata ttagcaaaaa
    23941 aacaggtgac caaactttcg ggaggtcagc agcagcgagt cgctatcgtt cgtgctatct
    24001 gctgtgaaca tcaactaatc gtagcggatg agcctactgg aaatctagat gaaacaactt
    24061 cacaagatat cgtcaaactt ttccaagaaa tcgcccatga acaaaatcgc tgtattatca
    24121 tcgtgactca tgagattgaa gtagcaaaaa gctgtgatga agtctttgaa ctaaaaaata
    24181 aaaaatttca aaaaatcgaa gtttaaaaaa caggataatg atcaaataaa aaatccgaac
    24241 catatctgtc tcgcagatat ggttcggatt ttttatttgc ttgattcaat tagtaataag
    24301 tggtttttat tttttttcgt cttttcgaga aaacttgtct ttgacttcgt cgcctaaatt
    24361 tcgagctttt tctttgatgt cagcacctag tgattgtgct ttcccttctg tttctttgct
    24421 cttgtcgtct gttgctttgc cgtatgcttc ttttccttca ccgactactt tatctttcat
    24481 atctttagaa cgtcctttta aatctgccat tgtgcttcct cctcttagtt tgtttattga
    24541 tctaccttca gggtaacatc aacaacagat acagacaaac agaacgctcg ttttctaaaa
    24601 aagatagtta gagctgttga ttaaaaactt ttttcgaaag tcccccatac tttgacttct
    24661 ttttcttgga ccattatttc tcctttagcg ataactgtat caatatcaag cgtttcttta
    24721 tccaagatac acagatctgc atcagcacca atttcgattt ttcctttttt atccaatttc
    24781 aatatacgtg ctggattact ggttatagct cgtaaggcga tttccaaagg gatactttct
    24841 ttttgcacag cttctttgat ccctactagc aatgccttgg cacttcctac tcctaaccct
    24901 aggaaatgat tgttttcatc aaaataaggc aagcttcctt gtccatctga ggtcatcgtg
    24961 aaattatcta aagaaacatc ttcctcgatc aatcgtttca gtgctttgct gaagcgaact
    25021 tcaccatctg tcttctccca aaaatcagga tcttcgctag cagtgaagtc aatcgtgcca
    25081 ccacgtttgg caaatgccac gcaagcttca aataattctg gtgtgcgatt tgcatgagta
    25141 ggcaaaaatt gtgtgatcgg aatttctgtt tcttctacga ttcgtgtcaa tagctctagc
    25201 tttctttttc ctccgcctaa gtggacattg atgatcccag cttttccgga taacatgcct
    25261 cctacacgag catctgctac tgcacgagcg aactcttcaa aacttggctg agaagaacgg
    25321 tgatcggata ctgcgatttc tccgattccg ataattttat caattgtcaa aaagtctttg
    25381 atgatagaat ctgttactgt ttttaccggc aatcggtacg aaccttcgta tacataagtg
    25441 gagattcctt ctgcttccag acctttcgct ttgctgatca acgcagtcat atctctacct
    25501 tctccgtctg ttcccaaaca accgacaact gtcgttacac cagctgtagt caattggctc
    25561 aatgtgactt ctggcgttcg attttgataa ccgccttctc ctcctccacc taaaatatgg
    25621 aaatgacagt caatgaaacc cggggttagt acttttccct cacctgaaag ttcttctact
    25681 tccactcctt cgtagccgcc ttctagctgt tcttctacag ccaaaatctt cccaccggca
    25741 atcagtacat ccatttttcc taagtaatct ggtgcaaaca cttcaatttg tcgaatgatt
    25801 ttcatacatt tttcctcttt ctttaagtat aactgatgat aggatcttaa aaaataatcc
    25861 aaacgatagg atctttactc ctttgatttt accgtaaaat gacggttctt ggagtcctta
    25921 tctactgttt ggattacttt tttatttaca ggtctccagt actactgcag acctattgca
    25981 atggcaatca tgatgaaaat agttcctaaa acaaacagga atccttgcat cttgatttgg
    26041 aatcttgccc attttgccca ttcgatccgt gcaacaccta gcacaccaat caagctggct
    26101 gatacgggtg taaaagcgtc cataaaacct gaaccaagct gataagcaag aactgctact
    26161 tgtctggata cacctactaa atctgccagt ggagccatga ttggcatagt caaagctgct
    26221 tgtcctgaat ttgatgtaac aaccaagttg aacaaacttt ggaagatgta catcgcccaa
    26281 gcaccaatga cagctggaac accggtcaac gccgtcccga tcccatgcaa aatcgtattc
    26341 aaagctgaag gaacagatgc atcagatcct cccaagacaa gaagaatccc tttggccata
    26401 cctactacga ttgctgtacc agctaaatca gctgcaccag attggaaaga agaagcgatg
    26461 tcattgactt tcattccatc taatttgaaa agaacagcaa tgattccagc aactaatccc
    26521 atcacaaaga attgagaagc gatttctgga atgtaatagc ctttttgagt cacgccccaa
    26581 acgatccaga caaggacaac taacatctct aataagatca atttatgccc aagcatgaat
    26641 tttttatttt catcagatgt ctttttgata tgagaacgga aataatcatc tgatttgtaa
    26701 gtcaacgaac tttctggttt tttacggatt ttttctgcat atatcatcat ataaccagcg
    26761 gctaatgcag taacaactac ccacataatc aatcggaatg tcgcaccaga caacacgggg
    26821 attccagcaa tcccttgagc caccgctacg ctaaacgggc tcatccaaga ggtcgcattt
    26881 ccgacttgag atgcgacata agtcacggtt actgctacga tcgagtcata tcctaacgca
    26941 atgacaaatg gaaccatcac cattgagaat ggaattacct cttctgccat cccgaatgta
    27001 gcgccaccga atgaaaaagc aaagaataat agcggtaaag ctaaacgctc cagtccctta
    27061 gttttactga taaacgcata gatacctgcg tcgatcgctc cggtacgcat aataatacca
    27121 aaagcacctc caacaacaag gatcaatgca gcgatcccta cagcagatcc atacttatct
    27181 ccagatacta gtccttcaaa aacaaaattt aagaaaccaa agccgccaaa atcatctgtt
    27241 ccccatattt tggctgtttt atgcaatttt ttagaagtat catagatatt ttcgccgtac
    27301 tgatcgtaaa gcacatcatc tgtcaaggag atttcatcta gtttttcctg cgtaagattt
    27361 ttttctccgt cagcaatgac tttttccaat cctttttccg gaacatccag tttttcacgt
    27421 tctgctggat gatcttgcag ttcttctaat tgatcaaaca catacgactt atccagctcg
    27481 taagcataac ggaaggaatc ttgacgtaaa acagtacgag tacttgtttc gccatttgca
    27541 tctttgtatt caatatcttg cgtgctgaac tttcctgctg gaacgacaaa agtcagtatc
    27601 catgcaaaga tgactacacc aaaaataatg acatacgtat ggggtgtcga taatttagtg
    27661 aagtcgcgtt tcggttttcc tgaagaattt gttttcttca aatttatttc ttcctttctt
    27721 ttttcagtaa ttttgatgaa taaaaaatgg ataatcaaat gaattataca gtaaaaatga
    27781 catttccaca tgaataattt attagaaaat cacttttttc tattttgttt ttttctccta
    27841 tgtatgatta tttttttctc tttccattta tattaagtat tttatttctc tgtttttcct
    27901 taaaattctt tttttatttt tagattagag aaaatctcgt cctgtttttt tcattttaca
    27961 atcattgaaa aattgaattt cattgcaaaa aaaataataa acacttataa atatccatta
    28021 tctatccttc aatttttgtg tattttattt ctattagaaa aaggtatcct gttggacaat
    28081 tttgttatat caggtaaact tcattgcaat aatgataaca aatgtactat aattactttg
    28141 taaagagttt tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat
    28201 atggattctt tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt
    28261 ataactcatt tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca
    28321 agcttaaaat caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt
    28381 aatcctcatt aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat
    28441 ttttttaagg aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc
    28501 tgttttgctg taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt
    28561 ccgcagatcg ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg
    28621 ttgtccaaaa ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg
    28681 atacttcgtg acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc
    28741 cactgagcta gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg
    28801 tcttctgatt tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca
    28861 tagacacctc cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc
    28921 caaaattcaa atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct
    28981 tgattaattt gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt
    29041 aatttctctt ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc
    29101 ctagctatac ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac
    29161 agtagttaga cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac
    29221 gaatacgccc aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct
    29281 acataggggt agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat
    29341 tgactatggc aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt
    29401 agcgccaact aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg
    29461 taagtaatct cttatccaag tgcaagcaag aaaatacaaa aaaacattgg tatgatttat
    29521 atatatataa ataacataaa aaaaggaatg gcttatcccg atacaacaag cattccttta
    29581 aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat aaatctctca
    29641 tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa tattaccgtc
    29701 ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta gttgtatgtc
    29761 taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg attagataca
    29821 gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc aattcaacca
    29881 tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac gtttcttttt
    29941 ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac acaaatatcc
    30001 aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca acagcgacta
    30061 attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct ttttattcta
    30121 ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt ttatacaact
    30181 taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa agtctttttc
    30241 tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac gacagttgcg
    30301 caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca gcaggccact
    30361 tatttccagt taactttctg ttcttagctt tttactcaac tggataatat atgcacttaa
    30421 tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca tatatcctaa
    30481 cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg cctctgtctg
    30541 gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg gttcttgagt
    30601 ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg ctaaattact
    30661 tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac gacccttttc
    30721 gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg gtacacgcat
    30781 gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt gagtcaatgg
    30841 gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat ttcctataaa
    30901 tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac ggatgaagtg
    30961 aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt ccttttcccg
    31021 caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc ttttttcttt
    31081 tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca caataaactg
    31141 gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg ttgccggcaa
    31201 aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca cttgctgttc
    31261 ctcctaaacg acgattaact attgatcatc tatttaacga agagaaaatt cattctccat
    31321 ttttccgtca cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa
    31381 aacattatag attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac
    31441 tgctgctttt gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc
    31501 cagcttatgt ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt
    31561 acaagtaacg atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc
    31621 catcgcgaca ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc
    31681 atcatctccg aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag
    31741 tgctgcacca gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt
    31801 catgaacagt ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt
    31861 tgtgatatca tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc
    31921 ctgtcttttt tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc
    31981 ttgttctagt cctttacttg catgtataat gattggctta gtattcattt taccaataag
    32041 ttcctgtgcg actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt
    32101 atccactgct tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg
    32161 taaaaaatgc tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc
    32221 ccaaatacgg acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga
    32281 tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata aataattgat
    32341 aatttccatt atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt
    32401 ttgtttactt cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac
    32461 attttatttt tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg
    32521 ttactaccag cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa
    32581 atacataaga gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt
    32641 ctctgttgac ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct
    32701 atgtaaactg gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa
    32761 atagtactat tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata
    32821 agttgaactg tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta
    32881 aaaaaataaa gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt
    32941 ctatcctaat aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac
    33001 tgctccacat gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca
    33061 cgtccgcgat atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta
    33121 taattgctgc aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca
    33181 tcttcgccta aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga
    33241 agaaactcgg cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac
    33301 atggcatatg aataaacagc tccccattga ttccctgctc caataaaata atgcatacta
    33361 tcacgtcgat gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc
    33421 actaaccctg ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat
    33481 tctccattca aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca
    33541 ggagctggat aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg
    33601 ataaatccaa tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct
    33661 tgagttgtac cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt
    33721 ccagtagtgt aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc
    33781 atataagtga cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca
    33841 gcgtcaaatt tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct
    33901 agtgcgcctt ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc
    33961 tcttataatt tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca
    34021 atacctttag ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat
    34081 acagacagat attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt
    34141 tttgtggtaa tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc
    34201 gaacgttcct tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc
    34261 tccaggtatg ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt
    34321 gtttcagtca atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg
    34381 gtgacgaaac gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt
    34441 ttttgaataa tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt
    34501 tctatttttt tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac
    34561 tgtaaaacag tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct
    34621 aaatcaataa tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca
    34681 acaacaggta acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc
    34741 ccgctttgtg acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa
    34801 aaataaaaag gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc
    34861 gctgctaaac aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt
    34921 ttcatatgtt ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt
    34981 tcttcataga tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc
    35041 tatccacgaa ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac
    35101 ttctttcttc aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac
    35161 gatatcatca aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg
    35221 aatattgatg gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga
    35281 cgcgcctaat gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt
    35341 tcctaagcga tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa
    35401 aatcaaaatg aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac
    35461 acctgtggca atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg
    35521 tcccatcaaa gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt
    35581 attttcttcc tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt
    35641 gttaaaaaac tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa
    35701 ctttttcaaa atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt
    35761 ccaagaaaac tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat
    35821 tttgctgttt ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa
    35881 gtggtagttg acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa
    35941 tagctactcc ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat
    36001 atggtgcaac ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa
    36061 tcaaccctgt agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat
    36121 ttccgatcgt attgcttccc accatataag aaaccattac cacaacacca agcatcaagg
    36181 aaagaccgat accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg
    36241 cataattgtc tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca
    36301 atatcaatgt caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc
    36361 cagctgtaat agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg
    36421 aagcacctac tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc
    36481 cagtttttac atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa
    36541 gcaacgatgt ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga
    36601 ctgcttgtac aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt
    36661 ttatctgctg gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag
    36721 cgttcctcct cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt
    36781 gccttgatcc ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat
    36841 gcatcctcga tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc
    36901 aacgcggcaa tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct
    36961 aatttgatcg ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata
    37021 cagtctgcgc ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg
    37081 tcgactcttg tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct
    37141 gattgagccg ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga
    37201 tcaaaacagt tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca
    37261 aattcaatcc ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt
    37321 tgacacttcc gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa
    37381 aaagctgcct tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag
    37441 taatagaatg atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt
    37501 ttccatggga agctaagata atttctctat ccactttttc atctcctctt ttcacttaca
    37561 catactaaaa aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa
    37621 ttcttcttct atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat
    37681 ctcagaattg gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat
    37741 agcagataac ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat
    37801 tggcatttgt cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt
    37861 tttgggattg ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat
    37921 ttctcccatc accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga
    37981 aaaattacga atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc
    38041 caaccattca gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata
    38101 gtcgatattg gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga
    38161 aaataatggg tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt
    38221 caactgacaa gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc
    38281 gattccagtg aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag
    38341 tcgccattct ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt
    38401 tttttgcagg cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa
    38461 aagcggtacg gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc
    38521 tactaatatg atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga
    38581 agcaatctgc tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg
    38641 taataaacga tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat
    38701 catcgctttt ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag
    38761 taaaatccga tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat
    38821 tcgttggaca tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt
    38881 gattagttgg ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag
    38941 atagtaactc aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat
    39001 ttgtcgaaag gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg
    39061 tctactggtc tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc
    39121 ttcgcaagaa tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact
    39181 tttaacgatt cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc
    39241 ttgcgtaata tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc
    39301 tttttgcaaa tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc
    39361 ttttgctgct gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg
    39421 acttgttaaa agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg
    39481 ttcttcgaga aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag
    39541 tgcaggtagt tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt
    39601 aatctcttca gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc
    39661 tcccatacga taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg
    39721 tgttaagcga tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc
    39781 ttttttattt tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt
    39841 caaaagttca ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc
    39901 ttctagcaga tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc
    39961 tgtgccactt tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt
    40021 tttgatttgt tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa
    40081 gtcttttggt tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa
    40141 attcgtgcgt aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg
    40201 tcgagaattg attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac
    40261 tgtgtttctt tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat
    40321 atgttctaaa tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa
    40381 caacttattc acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta
    40441 ataaagaatt gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt
    40501 tattctaaaa aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga
    40561 ataaggaggc tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc
    40621 ctggaaaact tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt
    40681 gctttatatg gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc
    40741 cgaaacagga tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac
    40801 aaaataagat tcacagattt caactttcct gtctgttttt agctattaca aatccttata
    40861 tgtggtataa caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag
    40921 agtaaagagg tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt
    40981 ttcccattga ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt
    41041 tattttcaga tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc
    41101 catcaaacct gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct
    41161 aagatagtac ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa
    41221 aatcaattag atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc
    41281 aagaacaaat taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt
    41341 ttttatgcag aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga
    41401 gactggcatg ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag
    41461 cttcttcata ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc
    41521 ctaagctaca atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga
    41581 atcatatgat tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc
    41641 cgttcttcaa atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct
    41701 tgatcgataa tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga
    41761 tggtcgctat gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag
    41821 tagcttcaca aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt
    41881 tctctcacaa cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg
    41941 acatcaatca gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt
    42001 ttttaagtat cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt
    42061 tcttagcatt attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc
    42121 ctctctacca aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc
    42181 ttagcggtgt tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg
    42241 ataaaagcac aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat
    42301 tatcacctgc tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg
    42361 ggatcaaagt aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata
    42421 tgaatcaaat gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg
    42481 ccgaagcttc aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag
    42541 ctgaggtcag tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg
    42601 acttccgtta tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc
    42661 gaaatacagt ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat
    42721 tgattccgcg tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc
    42781 gttcttggca gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac
    42841 tatttaccgg gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag
    42901 aatgttggga agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg
    42961 gtttagatac aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac
    43021 tgacgattgc aagagctttg attgccaagc cagcattact gattttagat gactctttaa
    43081 gtgcgttgga ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca
    43141 gtacagtgat tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag
    43201 tgatggatca aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag
    43261 cagaatatca ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt
    43321 atccttgcag acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg
    43381 gctagctgtg atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat
    43441 tggtcaaagt atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca
    43501 tttacttatt ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca
    43561 agtgttaagc aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc
    43621 tcatctgaat cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag
    43681 tcgatttaca aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt
    43741 attttctgga gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact
    43801 tctgacattg gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc
    43861 tcgtgcttca caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt
    43921 tgtgaccgaa cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag
    43981 tcaacagcaa tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt
    44041 ttcttcttcc ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat
    44101 tggtttagtt ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat
    44161 ttccagcttt acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat
    44221 cacgacacaa ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca
    44281 gccagaagaa aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg
    44341 aaaagtctct tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga
    44401 tttcaatttt actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg
    44461 aaaatccaca cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac
    44521 agtcgatgac catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat
    44581 ggtgttacaa gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa
    44641 tccggaagca agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt
    44701 tgtagaacgt ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc
    44761 agatggacaa cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt
    44821 gatcttagat gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc
    44881 cttcttagca atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat
    44941 caaaaatgcg gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca
    45001 tgatgagctg cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc
    45061 acaataaaaa caagcagtct tctgggatgg gaagactgct tgttttt
//
LOCUS       DACIFS010000017        41205 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.017, whole genome shotgun sequence.
ACCESSION   DACIFS010000017 DACIFS010000000
VERSION     DACIFS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.017"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(26..328)
                     /locus_tag="IYA83_000950"
     CDS             complement(26..328)
                     /locus_tag="IYA83_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586401.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IYA83_000951"
     CDS             complement(353..2446)
                     /locus_tag="IYA83_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ8586402.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IYA83_000952"
     CDS             complement(2935..3699)
                     /locus_tag="IYA83_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ8586403.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IYA83_000953"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IYA83_000953"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ8586404.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IYA83_000954"
     CDS             complement(4704..5780)
                     /locus_tag="IYA83_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ8586405.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IYA83_000955"
     CDS             complement(5804..6397)
                     /locus_tag="IYA83_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ8586406.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IYA83_000956"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IYA83_000956"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ8586407.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IYA83_000957"
     CDS             complement(8067..8366)
                     /locus_tag="IYA83_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ8586408.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IYA83_000958"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IYA83_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ8586409.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IYA83_000959"
     CDS             complement(10121..11032)
                     /locus_tag="IYA83_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ8586410.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IYA83_000960"
     CDS             11152..12009
                     /locus_tag="IYA83_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8586411.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IYA83_000961"
     CDS             complement(12196..12558)
                     /locus_tag="IYA83_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586412.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IYA83_000962"
     CDS             12904..14343
                     /locus_tag="IYA83_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8586413.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IYA83_000963"
     CDS             complement(14350..16902)
                     /locus_tag="IYA83_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ8586414.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IYA83_000964"
     CDS             complement(17016..18347)
                     /locus_tag="IYA83_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8586415.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IYA83_000965"
     CDS             complement(18313..19791)
                     /locus_tag="IYA83_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8586416.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IYA83_000966"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IYA83_000966"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ8586417.1"
                     /translation="MKDMDIKVVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IYA83_000967"
     CDS             22401..23828
                     /locus_tag="IYA83_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ8586418.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLHEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IYA83_000968"
     CDS             complement(23920..24285)
                     /locus_tag="IYA83_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ8586419.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IYA83_000969"
     CDS             complement(24282..24647)
                     /locus_tag="IYA83_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ8586420.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IYA83_000970"
     CDS             complement(24839..25087)
                     /locus_tag="IYA83_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586421.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IYA83_000971"
     CDS             25086..25214
                     /locus_tag="IYA83_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ8586422.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IYA83_000972"
     CDS             25271..26965
                     /locus_tag="IYA83_000972"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ8586423.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IYA83_000973"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IYA83_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IYA83_000974"
     CDS             27170..27361
                     /locus_tag="IYA83_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586424.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IYA83_000975"
     CDS             27739..28872
                     /locus_tag="IYA83_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ8586425.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IYA83_000976"
     CDS             28903..29223
                     /locus_tag="IYA83_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586426.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IYA83_000977"
     CDS             complement(29252..29716)
                     /locus_tag="IYA83_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586427.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31100)
                     /locus_tag="IYA83_000978"
     CDS             complement(29847..31100)
                     /locus_tag="IYA83_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586428.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31129..31446)
                     /locus_tag="IYA83_000979"
     CDS             complement(31129..31446)
                     /locus_tag="IYA83_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ8586429.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31472..31792)
                     /locus_tag="IYA83_000980"
     CDS             complement(31472..31792)
                     /locus_tag="IYA83_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586430.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32256..33671)
                     /locus_tag="IYA83_000981"
     CDS             complement(32256..33671)
                     /locus_tag="IYA83_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8586431.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33688..35004)
                     /locus_tag="IYA83_000982"
     CDS             complement(33688..35004)
                     /locus_tag="IYA83_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586432.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35154..35852)
                     /locus_tag="IYA83_000983"
     CDS             complement(35154..35852)
                     /locus_tag="IYA83_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ8586433.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36141..37130)
                     /locus_tag="IYA83_000984"
     CDS             complement(36141..37130)
                     /locus_tag="IYA83_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ8586434.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IYA83_000985"
     CDS             complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IYA83_000985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ8586435.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38218..39852)
                     /locus_tag="IYA83_000986"
     CDS             complement(38218..39852)
                     /locus_tag="IYA83_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586436.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39839..41173)
                     /locus_tag="IYA83_000987"
     CDS             complement(39839..41173)
                     /locus_tag="IYA83_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586437.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12502 a         8475 c         6957 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacgac cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt acatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
    30841 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
    30901 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
    30961 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
    31021 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
    31081 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
    31141 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
    31201 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
    31261 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
    31321 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
    31381 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
    31441 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
    31501 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
    31561 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
    31621 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
    31681 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
    31741 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
    31801 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
    31861 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
    31921 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
    31981 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
    32041 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
    32101 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
    32161 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
    32221 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
    32281 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
    32341 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    32401 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    32461 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    32521 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    32581 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    32641 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    32701 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    32761 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    32821 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    32881 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    32941 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    33001 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    33061 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    33121 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    33181 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    33241 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    33301 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    33361 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    33421 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    33481 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    33541 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    33601 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    33661 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    33721 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    33781 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    33841 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    33901 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    33961 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    34021 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    34081 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    34141 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    34201 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    34261 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    34321 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    34381 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    34441 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    34501 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    34561 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    34621 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    34681 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    34741 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    34801 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    34861 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    34921 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    34981 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    35041 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    35101 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    35161 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    35221 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    35281 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    35341 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    35401 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    35461 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    35521 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    35581 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    35641 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    35701 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    35761 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    35821 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    35881 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    35941 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    36001 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    36061 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    36121 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    36181 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    36241 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    36301 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    36361 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    36421 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    36481 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    36541 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    36601 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    36661 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    36721 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    36781 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    36841 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    36901 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    36961 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    37021 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    37081 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    37141 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    37201 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    37261 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    37321 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    37381 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    37441 taattctttt tgtcgttcgc cttgataaaa aatcactgga cgatattgct tccctctatc
    37501 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    37561 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    37621 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    37681 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    37741 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    37801 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    37861 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    37921 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    37981 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    38041 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    38101 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    38161 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    38221 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    38281 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    38341 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    38401 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    38461 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    38521 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    38581 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    38641 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    38701 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    38761 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    38821 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    38881 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    38941 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    39001 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    39061 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    39121 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    39181 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    39241 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    39301 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    39361 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    39421 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    39481 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    39541 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    39601 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    39661 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    39721 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    39781 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    39841 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    39901 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    39961 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    40021 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    40081 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    40141 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    40201 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    40261 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    40321 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    40381 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    40441 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    40501 tacctaagat acgccaagga aactggagca agcttatcgg tgttttttga aacaaaaacc
    40561 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    40621 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    40681 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    40741 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    40801 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    40861 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    40921 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    40981 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    41041 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    41101 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    41161 aagcagcatc tatcttttta aaaaactttt ctccataata aaaac
//
LOCUS       DACIFS010000018        40742 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.018, whole genome shotgun sequence.
ACCESSION   DACIFS010000018 DACIFS010000000
VERSION     DACIFS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.018"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            23..646
                     /locus_tag="IYA83_000988"
     CDS             23..646
                     /locus_tag="IYA83_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586438.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IYA83_000989"
     CDS             1096..2145
                     /locus_tag="IYA83_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586439.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IYA83_000990"
     CDS             2142..3023
                     /locus_tag="IYA83_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586440.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IYA83_000991"
     CDS             3026..3796
                     /locus_tag="IYA83_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8586441.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IYA83_000992"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IYA83_000992"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ8586442.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IYA83_000993"
     CDS             5107..6270
                     /locus_tag="IYA83_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ8586443.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IYA83_000994"
     CDS             6387..6797
                     /locus_tag="IYA83_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ8586444.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IYA83_000995"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IYA83_000995"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ8586445.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVILYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IYA83_000996"
     CDS             9337..9654
                     /locus_tag="IYA83_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586446.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IYA83_000997"
     CDS             9814..10050
                     /locus_tag="IYA83_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8586447.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IYA83_000998"
     CDS             10047..10445
                     /locus_tag="IYA83_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ8586448.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IYA83_000999"
     CDS             complement(11112..12056)
                     /locus_tag="IYA83_000999"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ8586449.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IYA83_001000"
     CDS             complement(12352..13296)
                     /locus_tag="IYA83_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586450.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IYA83_001001"
     CDS             13517..14731
                     /locus_tag="IYA83_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8586451.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IYA83_001002"
     CDS             14753..16768
                     /locus_tag="IYA83_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ8586452.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IYA83_001003"
     CDS             complement(16851..18224)
                     /locus_tag="IYA83_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ8586453.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IYA83_001004"
     CDS             complement(18250..18534)
                     /locus_tag="IYA83_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586454.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IYA83_001005"
     CDS             complement(18536..20551)
                     /locus_tag="IYA83_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ8586455.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IYA83_001006"
     CDS             complement(20730..21035)
                     /locus_tag="IYA83_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ8586456.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IYA83_001007"
     CDS             complement(21124..22155)
                     /locus_tag="IYA83_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8586457.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFPLAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="IYA83_001008"
     CDS             22362..23237
                     /locus_tag="IYA83_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ8586458.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="IYA83_001009"
     CDS             complement(23389..24231)
                     /locus_tag="IYA83_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ8586459.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="IYA83_001010"
     CDS             complement(24323..26200)
                     /locus_tag="IYA83_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ8586460.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="IYA83_001011"
     CDS             complement(26197..27618)
                     /locus_tag="IYA83_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ8586461.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="IYA83_001012"
     CDS             complement(27621..31010)
                     /locus_tag="IYA83_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ8586462.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32586
                     /locus_tag="IYA83_001013"
     CDS             31267..32586
                     /locus_tag="IYA83_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8586463.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEEYVVQLLTQ
                     IYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSS
                     YIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITED
                     QLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(32659..33306)
                     /locus_tag="IYA83_001014"
     CDS             complement(32659..33306)
                     /locus_tag="IYA83_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8586464.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33314..34000)
                     /locus_tag="IYA83_001015"
     CDS             complement(33314..34000)
                     /locus_tag="IYA83_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586465.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34006..35079)
                     /locus_tag="IYA83_001016"
     CDS             complement(34006..35079)
                     /locus_tag="IYA83_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ8586466.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35301..35975
                     /locus_tag="IYA83_001017"
     CDS             35301..35975
                     /locus_tag="IYA83_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586467.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35980..37320
                     /locus_tag="IYA83_001018"
     CDS             35980..37320
                     /locus_tag="IYA83_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8586468.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37469..38455)
                     /locus_tag="IYA83_001019"
     CDS             complement(37469..38455)
                     /locus_tag="IYA83_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ8586469.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTYDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39179..40210
                     /locus_tag="IYA83_001020"
     CDS             39179..40210
                     /locus_tag="IYA83_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ8586470.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40319..>40742
                     /locus_tag="IYA83_001021"
     CDS             40319..>40742
                     /locus_tag="IYA83_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ8586471.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12642 a         7400 c         7647 g        13053 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcattcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactagat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgg
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga atgtaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat
    32101 tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt
    32161 gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa
    32221 ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg
    32281 atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc
    32341 agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct
    32401 gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat
    32461 cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat
    32521 atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag
    32581 ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat
    32641 acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa
    32701 gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat
    32761 tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa
    32821 atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc
    32881 tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata
    32941 gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa
    33001 atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct
    33061 tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg
    33121 cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca
    33181 aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa
    33241 agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat
    33301 tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc
    33361 gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt
    33421 tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat
    33481 ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag
    33541 caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg
    33601 actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag
    33661 taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc
    33721 tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct
    33781 cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg
    33841 aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc
    33901 cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa
    33961 atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc
    34021 tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac
    34081 aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc
    34141 gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac
    34201 cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg
    34261 tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc
    34321 gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg
    34381 ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt
    34441 cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg
    34501 ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga
    34561 cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa
    34621 aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag
    34681 attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg
    34741 taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt
    34801 gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc
    34861 tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc
    34921 ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc
    34981 tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg
    35041 taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata
    35101 gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca
    35161 cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac
    35221 atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac
    35281 caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat
    35341 ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta
    35401 gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga
    35461 tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc
    35521 cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag
    35581 gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag
    35641 cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca
    35701 gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa
    35761 aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc
    35821 agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt
    35881 atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct
    35941 tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga
    36001 aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt
    36061 ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca
    36121 ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa
    36181 ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca
    36241 ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt
    36301 gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg
    36361 gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct
    36421 gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac
    36481 atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt
    36541 atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct
    36601 agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat
    36661 gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt
    36721 accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag
    36781 acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat
    36841 cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa
    36901 ccatttcaag agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca
    36961 ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat
    37021 gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa
    37081 gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg
    37141 atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg
    37201 cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc
    37261 aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa
    37321 tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa
    37381 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca
    37441 cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta
    37501 catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa
    37561 catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta
    37621 catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg
    37681 ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga
    37741 ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag
    37801 ttgtcacatc atatgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat
    37861 ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc
    37921 caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa
    37981 agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc
    38041 ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg
    38101 gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt
    38161 gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag
    38221 tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg
    38281 tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag
    38341 tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata
    38401 atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa
    38461 ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt attctatagg
    38521 agacgattgt tttctacaaa tatttagatt ggaatttgat taaaacaatt taattgaaat
    38581 ttgaaaacat ttatgttaat gaaagtcata ttctaataaa atgtcatttg tttctatatg
    38641 aatttttatg aaaaatttca tataatttcc actcgttgtg atggtttaat ttatccaatc
    38701 catcaataag caaacaactt atataaaaaa ggattaattc tcaatataat tgctcctacc
    38761 atagaataaa tcttcatgta aagtaatgaa gtaccaacta tttctaatac ttctttaaga
    38821 attatcccaa caatatcgtt agacaaacca ttattgaata aatggagagc caatcgattc
    38881 tgaaaaaatg ttttctaatt tttcggaaat aaaagaaaga gcctcctgcc attagaacga
    38941 accaaatcca tttcattgct catgaagtaa ccacatagct aaaaagagga taaatcaatt
    39001 aaaagatcaa aagaatgact gcactaataa aataactaaa aatgtatctt cttacttcat
    39061 atcttttagc tagtacttag ttaaaaattt gcttcctaag atcaagacaa actgtctatc
    39121 ttcatttgat gaaatgcggt aaaatataga aaatgaacac tattggaggg aatttgtaat
    39181 ggacagcaaa actagaaaac aactgcaaga aaagttaaag caacgtacac acttgctccc
    39241 ttccgtcttt caacagtttt tagaagaaaa tcgttcttcc ctttcattag gcagtcgcta
    39301 tgaatacgca aaagattttc atctgtttac tgattatttg agaaaagtat tttctgaaga
    39361 agtggatgat cagctgatta tccatttaga aaaacaagat tatcagaatt tcttagctaa
    39421 aatctatcga catactcgtt cgttccaaac tacagcgaac caagaaacgg agcaattgtt
    39481 tgaaaatagc tattacggga tttcaaggaa gcaatattca ttaaatcatt ttttacgatt
    39541 tttgtatcaa aaaggactga ttgaagaaga gatttctctt ttgggtacaa gtttaccgga
    39601 aacaaccaaa gcagctccgc gatatatcga tgcagaactt ttcaaagatt ttgagcattt
    39661 gtttatcccg attgaaggct ttcaaacaag cagagaagct agctttgaag aaaagaatag
    39721 accgaggaac cttgcaatta ctgccattct tctgtattgc ggattaaaga tccatgaagt
    39781 agtagaactt aaacgtaaag atgtatcttt gaccaagcag attcttaatc tgaatagaaa
    39841 agaaaacaga ctgaataaag taccaatacc aaaagaagcg atgccatttc tggagaacta
    39901 ttttaagtta acagcacaaa atacggacga aaattctttt gtttttcgtt cctcacatga
    39961 tcagcaaatt tctcctcgga ctattcgcca aattttgagc aaaattacag tatataaaaa
    40021 cgtatcgcca gaactatgca gaaaaacata tcgttattat gcagaactat atttagatga
    40081 tgctgacgcg gtaaattatt tatgtggtaa tcgatacctc tcatcccatt cttttcaaga
    40141 tatctgggaa aagatggttg atttttctta tcacgaatta gcggcattcg ttcgggtttc
    40201 ttccatataa aacaaatgta tgatcttttt ttcacaataa actatgttat tatataaagg
    40261 attatgttac gattatcttg aatttgtata ttacttaaca aaagataatg gagtgtgaat
    40321 gatggagacc ataacgtatt cccctttgaa cttatttacg aattttcaag aagctgcagc
    40381 taacttttcc gagaccccga ttatctttga tgagccactt tcagctttcc cagaattagg
    40441 acttgaaaca acttacaaaa aatgctgcga aagtgtgttg aaacgggctt atcaattggc
    40501 tcatcttgga gtgaaatccg gagacaaagt tattatttat aagagttccg cttttgacac
    40561 ataccttcta gctgtttctg tgtcttacct tggtgcagtg cctgtcatga cttcttatca
    40621 tctaccaaca acaacaatgg aagtatttat cgatcgttta gaagaccctt tcattttatt
    40681 tgatgatgag acaaaaaagc gagtgagaga aatttcaaat ggaacaaaat caaaacagat
    40741 tc
//
LOCUS       DACIFS010000019        40647 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.019, whole genome shotgun sequence.
ACCESSION   DACIFS010000019 DACIFS010000000
VERSION     DACIFS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.019"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(45..1928)
                     /locus_tag="IYA83_001022"
     CDS             complement(45..1928)
                     /locus_tag="IYA83_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ8586472.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2194..3069)
                     /locus_tag="IYA83_001023"
     CDS             complement(2194..3069)
                     /locus_tag="IYA83_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8586473.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYA83_001024"
     CDS             complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="IYA83_001024"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ8586474.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4962..6254)
                     /locus_tag="IYA83_001025"
     CDS             complement(4962..6254)
                     /locus_tag="IYA83_001025"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ8586475.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSIPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6648..7595
                     /locus_tag="IYA83_001026"
     CDS             6648..7595
                     /locus_tag="IYA83_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ8586476.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7684..8943)
                     /locus_tag="IYA83_001027"
     CDS             complement(7684..8943)
                     /locus_tag="IYA83_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ8586477.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9114..9560)
                     /locus_tag="IYA83_001028"
     CDS             complement(9114..9560)
                     /locus_tag="IYA83_001028"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ8586478.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9571..11784)
                     /locus_tag="IYA83_001029"
     CDS             complement(9571..11784)
                     /locus_tag="IYA83_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ8586479.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12017..12781)
                     /locus_tag="IYA83_001030"
     CDS             complement(12017..12781)
                     /locus_tag="IYA83_001030"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ8586480.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYA83_001031"
     CDS             complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="IYA83_001031"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ8586481.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13742..14230)
                     /locus_tag="IYA83_001032"
     CDS             complement(13742..14230)
                     /locus_tag="IYA83_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ8586482.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14196..14867)
                     /locus_tag="IYA83_001033"
     CDS             complement(14196..14867)
                     /locus_tag="IYA83_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ8586483.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15005..16291
                     /locus_tag="IYA83_001034"
     CDS             15005..16291
                     /locus_tag="IYA83_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ8586484.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16363..16557
                     /gene="ssrS"
                     /locus_tag="IYA83_001035"
     ncRNA           16363..16557
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IYA83_001035"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16635..16919
                     /locus_tag="IYA83_001036"
     CDS             16635..16919
                     /locus_tag="IYA83_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586485.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            16992..17471
                     /locus_tag="IYA83_001037"
     CDS             16992..17471
                     /locus_tag="IYA83_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ8586486.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17694..17837
                     /locus_tag="IYA83_001038"
     CDS             17694..17837
                     /locus_tag="IYA83_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ8586487.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18415..19998
                     /locus_tag="IYA83_001039"
     CDS             18415..19998
                     /locus_tag="IYA83_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ8586488.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20435..21619)
                     /locus_tag="IYA83_001040"
     CDS             complement(20435..21619)
                     /locus_tag="IYA83_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ8586489.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21644..22651)
                     /locus_tag="IYA83_001041"
     CDS             complement(21644..22651)
                     /locus_tag="IYA83_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8586490.1"
                     /translation="MLPEKMEQGFNQKLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22823..23182)
                     /locus_tag="IYA83_001042"
     CDS             complement(22823..23182)
                     /locus_tag="IYA83_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586491.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23154..23525)
                     /locus_tag="IYA83_001043"
     CDS             complement(23154..23525)
                     /locus_tag="IYA83_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ8586492.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23542..23844)
                     /locus_tag="IYA83_001044"
     CDS             complement(23542..23844)
                     /locus_tag="IYA83_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586493.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(23861..24331)
                     /locus_tag="IYA83_001045"
     CDS             complement(23861..24331)
                     /locus_tag="IYA83_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ8586494.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24306..24584)
                     /locus_tag="IYA83_001046"
     CDS             complement(24306..24584)
                     /locus_tag="IYA83_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ8586495.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24581..25630)
                     /locus_tag="IYA83_001047"
     CDS             complement(24581..25630)
                     /locus_tag="IYA83_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ8586496.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYA83_001048"
     CDS             complement(25587..26516)
                     /gene="cpaF"
                     /locus_tag="IYA83_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ8586497.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26648..28402
                     /gene="ade"
                     /locus_tag="IYA83_001049"
     CDS             26648..28402
                     /gene="ade"
                     /locus_tag="IYA83_001049"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ8586498.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYA83_001050"
     CDS             complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="IYA83_001050"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ8586499.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29232..30011)
                     /locus_tag="IYA83_001051"
     CDS             complement(29232..30011)
                     /locus_tag="IYA83_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ8586500.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30218..31429)
                     /locus_tag="IYA83_001052"
     CDS             complement(30218..31429)
                     /locus_tag="IYA83_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586501.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31422..32159)
                     /locus_tag="IYA83_001053"
     CDS             complement(31422..32159)
                     /locus_tag="IYA83_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586502.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32297..32731
                     /locus_tag="IYA83_001054"
     CDS             32297..32731
                     /locus_tag="IYA83_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ8586503.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32724..33068
                     /locus_tag="IYA83_001055"
     CDS             32724..33068
                     /locus_tag="IYA83_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ8586504.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33248..34258
                     /locus_tag="IYA83_001056"
     CDS             33248..34258
                     /locus_tag="IYA83_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8586505.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDYLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34327..35271)
                     /locus_tag="IYA83_001057"
     CDS             complement(34327..35271)
                     /locus_tag="IYA83_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586506.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35255..37975)
                     /locus_tag="IYA83_001058"
     CDS             complement(35255..37975)
                     /locus_tag="IYA83_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8586507.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37972..39192)
                     /locus_tag="IYA83_001059"
     CDS             complement(37972..39192)
                     /locus_tag="IYA83_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ8586508.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39473..40318)
                     /locus_tag="IYA83_001060"
     CDS             complement(39473..40318)
                     /locus_tag="IYA83_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8586509.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12033 a         8442 c         7090 g        13082 t
ORIGIN      
        1 ttcaggcaaa aacaaatgtt tttgcctgaa gttttttgta gctatcattc tgtcacgttt
       61 gtctcactgc tgttcgtact tgaacttcct cctagcaagg cttcgctgac taagtgaatg
      121 taatgtgtca tcccttcagg atttggatgg acttgatcat cataaaacca gtcactatgg
      181 tcattactgt agctgtacca atcaatcagc gtcatattgt cgtatttttt cgtcatctgt
      241 tcaagcatcg cattcacgtc attttgccag cgcttcgtcg gtactcggac attgacccag
      301 tatacttttc gatcaccgat tgcatccatc acactatcaa actgagcttc cgagaatgag
      361 ccattcgttc ctagtccgat cagaacagtg tctttcaaca aatcttgttc ctttaatttt
      421 tcaatgtacg gtacactggc atataattgt cgtccgacat ctccatctac cactactttt
      481 ggataaagtt cttgtagatc cgttgcagca tccaacataa ctgaatcacc aaatgccgtt
      541 agttctaatt tttgtgcctt attcccttgt gccttggaca actcgtactt atcaaggatc
      601 gcttggtcaa ccgaactagt ggtgttttct gaattgtttc tggtttcttc tgctaatttt
      661 ttactttcag cgatttttgc ctgcagttgt ttttgatcgg ctgttacagc gtttttcggt
      721 gccgtaacga aaccaacgag tgcaattgct acaagtaatg tgcctggaac aacccatggt
      781 ttgctcttac tgatgatcgg tttgctgaac catcctttca tcacttggaa tgtacgacca
      841 taatcaaaac gtgctagcgg acgctccaca tatcgataag aaagttcact gatgattaga
      901 atcaatgtga tttccactaa agtatgcaac aaaacatgat cggcaatatc agtcaccttt
      961 gcttcataaa aaatcataac tggaaactga tacagataaa tgccatagct tcgtttgcca
     1021 atccaagtaa atactggatt tgtcagccat cgattcaaac ttgctccggg gtgtgctgtc
     1081 actgcaacta gcactacgca taacagactc actaggagca ttcctccgta atagatgaaa
     1141 ttgtaatgat catctaaaaa gaagaagcaa agaatcagtg ccaaaagcga gattcctcca
     1201 gcgatattca gaatattttt cgcttgtttc gggatgtttt tctttaaacg cgtacttggc
     1261 caaacaaacg caagcgcact tcccaaccat atagaaaaca gacgagtgtc ggtaccgtaa
     1321 taaacacggg ttggatcgcc tcctggtgta tacaagatca tcatcaagat ggcggagagg
     1381 aaggatgtac ctaacacagt ataaaagatc cattttcttt tacgaacaaa taccatcaac
     1441 aatacaaaca agatcggcca aaccaaatag ttttgacctt ctactgctaa agaccagata
     1501 tgcgtaaacg gtgactcatt agcaaatcga tcgaaataag ataaaccgtt attgatttgc
     1561 caccagttgt tgacatacag taagctgctg accacaactc ctcgaagatt gtttaacagc
     1621 cctcgctgaa acaaagtgat ataagccgaa gctgtcacaa gcataaaaac aagtgccgga
     1681 tagagtctct tcattcttct gacataaaat tgccaaatat tgatttttcc attttgttcc
     1741 cattcctgcc gcagcagatc cgtgatcaaa taaccagata cgacgaaaaa aatcggaacg
     1801 cccaaatagc cgcctttcat actagttggc agaaggtgat agaagataac acccaccaca
     1861 gctaatgaac ggagcccatc aaacccagtg atgtagcggc tgttttttaa ccttttttgt
     1921 ttttccattc aatattccaa cttttcataa acttttttca ttcgtaatat acgggtatta
     1981 agtgaaaata gcaagtagaa tcgtgtcctt tctctactat ttttttctat catcccataa
     2041 atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata aaaaagcagt
     2101 ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac gtctgtctac
     2161 agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat agaaacgacc
     2221 aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag ctttcattgc
     2281 ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg tctgcttatc
     2341 atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca tacagttttg
     2401 gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca tcttcttttg
     2461 tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg tactgtaaaa
     2521 catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat tgaagtagat
     2581 cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca cgatatctag
     2641 accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca tcacagcccc
     2701 cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta aaatatggat
     2761 acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct tcccgatttt
     2821 taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg ataccggtac
     2881 ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac cagtaatacc
     2941 acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg cgatcaacgc
     3001 ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat tttcgaatgt
     3061 ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt gtttttcaat
     3121 taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt ttatcggcta
     3181 ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg aaaccatact
     3241 tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa tgacttcacg
     3301 aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg caataccgcc
     3361 atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt cttccatttg
     3421 ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag tatgaatacg
     3481 gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt ctgcatagac
     3541 ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg tgaatggatg
     3601 gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc aatcagttac
     3661 ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta aacgtgtccg
     3721 gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca tcagcaagtc
     3781 acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca tgaatttagc
     3841 aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct tagctccgaa
     3901 ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat ctgcagcgcc
     3961 tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga agactttgaa
     4021 ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac gtgtatccgg
     4081 tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa atggcaatgt
     4141 gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg tgtaagtttg
     4201 gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt caggctgacg
     4261 gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac ggtcgaatcc
     4321 agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat agaaatgtcc
     4381 agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag atttccctag
     4441 gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa tcgcttttgt
     4501 tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat ccaaataacg
     4561 atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa atggcggggt
     4621 ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac cagtggccat
     4681 tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt cgatcacgta
     4741 ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag gattaaagac
     4801 gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc caaggtctcg
     4861 tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt cggaaactaa
     4921 tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa taaagtcatt
     4981 tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact tttttctttt
     5041 cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc accgatcacc
     5101 aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc tttacggttc
     5161 atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac tttcaatgct
     5221 tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt aaatgcaggg
     5281 ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat tgcaaatccg
     5341 aagccaggta ttgaaggacc acctagttct tccactagat tgtcataacg tccgcctgcg
     5401 caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa aatcgtatgt
     5461 gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac agccaatgca
     5521 tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag ataatctaag
     5581 atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc caatacgcgt
     5641 aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc aaaaggtaaa
     5701 agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc taatgaattg
     5761 atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc catcaccatg
     5821 ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat ttgatggaat
     5881 tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat ataaaaagtt
     5941 ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga acgaacgatt
     6001 ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata aaaatcatac
     6061 atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac ttcatagtgt
     6121 tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa acgtgctgtt
     6181 tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt tccttttggt
     6241 ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa aatcaccctt
     6301 gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt ttgaaagatt
     6361 tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg tccatctcca
     6421 gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc ctctcttgta
     6481 aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata acctactaaa
     6541 aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg ttttagttca
     6601 gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg gcagaaataa
     6661 gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat gccgttggag
     6721 gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt cgtacagctt
     6781 tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc cgttccgaag
     6841 aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc gtcatcgcta
     6901 caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct gagtttctca
     6961 aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac attttttata
     7021 tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg cacgaattaa
     7081 aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa caattgaaag
     7141 aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg atccatcggc
     7201 aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt gttccctatt
     7261 ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa aaacaatttg
     7321 gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc agagaaattt
     7381 taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact cctgcgcaac
     7441 ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct ggagctaagc
     7501 gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc aatgaagatt
     7561 atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc aatttatagt
     7621 aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata agtttatgct
     7681 aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact gattgaatgg
     7741 aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct gatcggaaaa
     7801 cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt gtcgaaaatt
     7861 tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt ttgctttccc
     7921 tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat catggtctgt
     7981 tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat ctacttctac
     8041 ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc tcatttcttg
     8101 aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca aatctggtct
     8161 actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg ttggacggat
     8221 gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat ctaaatctgt
     8281 ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat tttgtcgagc
     8341 ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga caccttttat
     8401 gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt ctgctaattt
     8461 ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc aaatagatgt
     8521 aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg cttcaagtgc
     8581 ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg gtgtcattag
     8641 atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct gtactccacc
     8701 ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt tgaaattcct
     8761 tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt ctttattgaa
     8821 aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca acatgttgta
     8881 tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg gtacttcttt
     8941 catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac tttttttccg
     9001 actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta gagtttatca
     9061 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
     9121 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
     9181 gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc attgaatgat
     9241 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
     9301 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
     9361 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
     9421 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
     9481 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
     9541 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
     9601 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
     9661 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
     9721 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
     9781 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
     9841 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
     9901 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
     9961 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
    10021 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
    10081 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
    10141 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
    10201 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
    10261 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
    10321 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
    10381 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
    10441 atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
    10501 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
    10561 tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac taaagatatc
    10621 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
    10681 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
    10741 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
    10801 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
    10861 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
    10921 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
    10981 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
    11041 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
    11101 cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
    11161 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
    11221 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
    11281 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
    11341 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
    11401 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
    11461 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
    11521 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
    11581 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
    11641 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
    11701 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
    11761 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
    11821 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
    11881 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
    11941 taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt cgatactctt
    12001 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
    12061 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
    12121 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
    12181 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
    12241 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
    12301 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
    12361 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
    12421 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
    12481 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
    12541 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
    12601 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
    12661 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
    12721 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
    12781 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
    12841 gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc ttctcttcaa
    12901 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
    12961 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
    13021 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
    13081 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
    13141 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
    13201 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
    13261 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
    13321 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
    13381 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
    13441 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
    13501 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
    13561 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
    13621 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
    13681 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
    13741 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
    13801 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
    13861 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
    13921 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
    13981 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
    14041 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
    14101 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
    14161 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
    14221 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
    14281 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
    14341 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
    14401 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
    14461 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
    14521 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
    14581 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
    14641 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
    14701 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
    14761 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
    14821 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
    14881 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
    14941 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
    15001 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
    15061 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
    15121 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
    15181 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
    15241 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
    15301 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
    15361 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
    15421 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
    15481 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
    15541 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
    15601 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
    15661 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
    15721 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
    15781 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
    15841 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
    15901 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
    15961 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
    16021 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
    16081 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
    16141 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
    16201 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
    16261 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
    16321 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
    16381 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
    16441 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
    16501 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
    16561 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
    16621 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
    16681 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
    16741 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
    16801 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
    16861 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
    16921 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
    16981 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
    17041 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
    17101 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
    17161 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
    17221 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
    17281 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
    17341 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
    17401 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
    17461 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
    17521 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
    17581 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
    17641 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
    17701 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
    17761 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
    17821 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
    17881 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
    17941 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
    18001 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
    18061 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
    18121 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
    18181 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
    18241 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
    18301 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
    18361 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
    18421 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
    18481 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
    18541 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
    18601 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
    18661 cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
    18721 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
    18781 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
    18841 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
    18901 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
    18961 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
    19021 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    19081 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    19141 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    19201 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    19261 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    19321 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    19381 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    19441 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    19501 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    19561 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    19621 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    19681 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    19741 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    19801 gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    19861 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    19921 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    19981 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    20041 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    20101 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    20161 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    20221 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    20281 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    20341 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    20401 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    20461 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    20521 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    20581 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    20641 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    20701 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    20761 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    20821 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    20881 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    20941 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    21001 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    21061 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    21121 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    21181 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    21241 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    21301 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    21361 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    21421 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    21481 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    21541 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    21601 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    21661 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    21721 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    21781 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    21841 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    21901 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    21961 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    22021 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    22081 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    22141 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    22201 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    22261 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    22321 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    22381 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    22441 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    22501 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    22561 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc agtttttgat
    22621 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    22681 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    22741 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    22801 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    22861 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    22921 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    22981 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    23041 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    23101 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    23161 tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    23221 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    23281 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    23341 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    23401 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    23461 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    23521 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    23581 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    23641 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    23701 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    23761 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    23821 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    23881 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    23941 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    24001 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    24061 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    24121 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    24181 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    24241 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    24301 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    24361 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    24421 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    24481 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    24541 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    24601 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    24661 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    24721 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    24781 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    24841 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    24901 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    24961 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    25021 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    25081 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    25141 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    25201 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    25261 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    25321 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    25381 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    25441 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    25501 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    25561 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    25621 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    25681 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    25741 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    25801 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    25861 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    25921 tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    25981 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    26041 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    26101 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    26161 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    26221 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    26281 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    26341 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    26401 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    26461 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    26521 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    26581 caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta tcttgggagg
    26641 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    26701 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    26761 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    26821 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    26881 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    26941 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    27001 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    27061 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    27121 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    27181 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    27241 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    27301 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    27361 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    27421 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    27481 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    27541 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    27601 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    27661 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    27721 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    27781 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    27841 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    27901 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    27961 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    28021 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    28081 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    28141 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    28201 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    28261 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    28321 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    28381 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    28441 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    28501 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    28561 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    28621 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    28681 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    28741 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    28801 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    28861 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    28921 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    28981 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    29041 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    29101 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    29161 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct ccgcagagtt
    29221 tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc gttcatccgt
    29281 cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat ggccgcatac
    29341 caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat cagccactga
    29401 ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac cgcagggtct
    29461 gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac taaccaatta
    29521 cgatggttta catctccgtg tactactgat aagttttcct tatctattga cgggatctgc
    29581 tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc ttccggtaat
    29641 tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc gatacgggtc
    29701 agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat cacgtcgttt
    29761 ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg tgctgtcaaa
    29821 gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc ttcccttgaa
    29881 agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc atcacgcatg
    29941 cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc ttggtctaac
    30001 tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta cttattattc
    30061 tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta attgccattc
    30121 aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt caacgctcta
    30181 tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc ttcttcaaac
    30241 gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc gctagcggaa
    30301 cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata accgacgcga
    30361 taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc ttatggattg
    30421 gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac agttggaaac
    30481 cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg actactccgg
    30541 caatcaataa cccacctata attactaatc ggaaataaag accactaaaa tcagaacctc
    30601 ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt ttaatatgag
    30661 tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca ggaacatctg
    30721 tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca tgcaccattt
    30781 tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat agacttgcct
    30841 gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga aacaaaaaga
    30901 tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga tctagtcgaa
    30961 acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat atgatcagat
    31021 agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc atggccaatc
    31081 cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa gctcgttcca
    31141 aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct gcctctgcaa
    31201 aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt atgagccctc
    31261 caattgcaaa cggtggctgc agggttttca accaattaga ataatataat cccacaccgc
    31321 caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc atatatttca
    31381 tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg tgcctgctcc
    31441 tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa ttcttttctc
    31501 aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac aaaacgatca
    31561 caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc tccttctttt
    31621 ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa gcctaaaaat
    31681 ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat catcactttc
    31741 tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga taaccgaaaa
    31801 gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc gtaggccatc
    31861 gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg agtctctgga
    31921 atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag atgattgatt
    31981 tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt cgtacttttc
    32041 cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc gaaactgata
    32101 tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat taaactcatt
    32161 ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac ggtattttgt
    32221 ttttttcctt acaatagctt taacttatgg taaagttaag ctgaagtatt tattctttat
    32281 agaaaggatg ttttatatgg aaaactgtat tttttgcaaa atcatcaatc aagaaatccc
    32341 aagctataaa atatatgaag acgacaaggt ctatgctttt ttagacatct ctcaagcaac
    32401 gaaaggacac acattagttg tgcctaaacg tcatgtagca gatatttttg aatatgatcc
    32461 cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat tggaaaaagc
    32521 ttttccagaa atgaaaggtc ttaatatctt gaataacaac cgtgaactag cctatcaatc
    32581 tgtttttcat tcacatatcc atttagtccc tcgctacagc aaagaggatg atttttctat
    32641 tcactttgtc aatcatcaag atagttatgg atcggaagaa ctaaaagcta ttcaagaaac
    32701 aatcgtaaag caggtgtcct gcgatgatta aaaacttttt caaaggactg ttatttggag
    32761 ctgctgctgg cactgtcgga ggtctgttat tggcgcccag gagcgggaat gaaacaagaa
    32821 agaaactgat cgaagaattg gaagaagcaa caactctgac aaatgattta aataacagcc
    32881 tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac agctgaatct gtgattcctg
    32941 ctttccaaga agcaatcaaa aaagatatcg aagattttaa attccaagcc gaaccacgta
    33001 tcacacaaat caatgaacaa gtagaaaaac taaaagctga tctgccagag tatcctgaac
    33061 aaatataata atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt
    33121 atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt
    33181 aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta caggagtgca
    33241 aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg ctgttttaac
    33301 tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag gcggaacgat
    33361 cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat ccttagtaca
    33421 acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa ccgataagca
    33481 agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg aatcacaatt
    33541 agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca atttagcttt
    33601 tgaagctggc ttgaaagctc acgtagatat tacagatgac tatttgaaaa ctgcttggaa
    33661 atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag atgaagcaaa
    33721 agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca aagacaaatc
    33781 aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa cgacaacaac
    33841 tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga tctccgatgt
    33901 tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca aaatggtgaa
    33961 aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag atatcgctac
    34021 tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag aattaaaaga
    34081 tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat tcacaacaac
    34141 ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt ctgacacaaa
    34201 atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat caaaataaaa
    34261 gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg ttttgaatga aaaaaataaa
    34321 tttttgctat aaatcttttg gtacatagaa acttcggttg tccataccga aaatccgatc
    34381 ggtgtatttt cctggttctg tttgacgaat cgaagtcaac atcatctgca tcgaagcatc
    34441 gatattgtcg atttgatgta atatttctgc ttccattatt cttggacgta caggtgagcc
    34501 atattctaat aagccatggt gtgctaaaac catatgacgt aatacgagaa catcttcgtc
    34561 agcttcgctg attttcaacg caagacaggc tttagttatt tcttcgtcaa tcaataccag
    34621 atgaccgatc aaatttcctg caagcgtata ttctgtcgac ataggaccgg aaagttcaat
    34681 cacctttcct aaatcatgca aaatgatacc agcatacaac aatgctcgat tcagctcagg
    34741 atattcatcg gcgattgatt tccctaagtg aagcattgtc aaagtatgat aagctaatcc
    34801 actggcaaat gcatgatggt ttctttttgc tgccggaaac tcaaaaaatt gtttttgata
    34861 tttattaagt agatagcgaa caatacgatt ccaatgcgca ttggtgatct caaacaacaa
    34921 ttgattgatt tcttcttcca tttcttcttt cttcaaaggg gctctttcca tgtataaact
    34981 aggctctcct ggctcatcta cccttgttaa gcgcatatga agaatcttta cttgagggtt
    35041 tccttgataa acctctcttt taccgtttag aaaaacgact gtgccggctg taaatttatt
    35101 gatttcctcc tccgaagcat cccagtattt accatcgata gtacctgaag tatcttggaa
    35161 agtaaaagca ataaattttt ttccgttttt agcgattctt acatctgcac ttttgatcag
    35221 gacaaattgt tcaaactgct catcgattac taaatcacga atttttttca ttatttgctc
    35281 cttctaattg ttttacgatt tggttcatct catggtaata gcttaccatt tctttatcag
    35341 aagaaaaaca aattatttga tacttttctc caaatttagc taaaagttca gctaattgat
    35401 actttcgctg atgatcataa tgaagccagc catcatcaat gatgatcggg catatcgaac
    35461 ggttagcttc taatgagagg taagcaaatc tcatcgccat aatcagctga tctttcgtac
    35521 ctgtagataa ctcatagatg ggaaattctg tatatccgtc tgttaaagtc agtgtatcat
    35581 ttttcagtaa tatcttctga taatttcctt ttgttaaaat acctaaataa tcacctgctg
    35641 ctcttaacaa ctggggtaat tgtctttcac tcatctctgt tgaaagatca cctaagaatg
    35701 tcccggcgat ttcaagtgct ccccattcct ccagtaattc ctgtaatttt gctttttgcc
    35761 ggctagcttg ttgataaagt tcgtccaatg tgccgtctct ttgcaactct tctacttgaa
    35821 gttggagtcg ttgccgattt tcacttgctt ctttttcttc ttgctggagt tgggattgct
    35881 tttctttcag ttggcgcagt cttacagata atttctcttt tgtcagctct tcaggaaata
    35941 gtggagtaag catttccgat aattcattgt atcttttcat ctgctgttcg gcttgtttgg
    36001 ctttttgtaa aaagacaggt acttccgaag gataagataa gcctgcttga gtcaattgtt
    36061 cgctgtattc ttgtatccat actttttgtt tctttttcaa ttgattgatt tcttgcttca
    36121 ataaagtatt ttgctgatag cttctggcaa atttcatctg ttccatctct tgtataaaac
    36181 gggaaagcaa taccattttt tctgaaattg tcttatcttg tatcggtaac cattctttaa
    36241 caaactcaaa tttttggtcg atttgtcgta gtttttcttg tatttcctgt tctcttttat
    36301 gtttccgctc aaatacgcca tataatttct gataatctat gacttcctgt ccatattctt
    36361 tcatgagttt gagcgtatcc atctttccta agtgccgatg atccgcaaaa cgatatactt
    36421 cttgctgaag aatctgtttt tcttctccag ttctcttttg tttttcttct gcttcagcta
    36481 attgagctaa ggctagatca agttgtccta gtttttcttg ccacattcct tttatctcag
    36541 agaaatcatt tttagtttta atttttggtt gccttttgta gaagaaatag ccaatcatgc
    36601 ctgcaccaat tagtaaaagg atcactttca agggcattgg tacaaatata cctgcaatga
    36661 ttattgctcc gccagccatc agcatatacg gtttagatga agagactggt gctatctctg
    36721 actgattttg taataatgct ggataggtct cttccaagtc attcacttct gcttcaatca
    36781 tctcttgctg ttctttagct atttggagac gcacagaaat ctggctttct agatcagcaa
    36841 tccttctttc ttcagccaga agatgcttcc attcttcttc atataaaaat tcttccggta
    36901 acggattttt ctgccaatgc cacctttgtt ccaaaatctt caattctgaa tttgtttctc
    36961 tagtctcaat ttgtaatctt tgccattctt cgtttaattg ttccgctgta aattgaagtt
    37021 gctggagatc ttttagttcc tgctcctgct ctaaataaaa ataatatctg gctgactgct
    37081 gatccatccc gctatgcttt tccagtgttg cttctaattc ttccaaacgc tcatttgttt
    37141 gctgataccg tttggagaaa gactccagat ctaaaagaag ttgctcatca ggaagacctg
    37201 tagtgctgtt ttttttgaga gattgccatt cttcatataa agaaaaatta agttcttgct
    37261 gtctggctaa tgaaaattgt ttgttcgcct cttttaattg tgccaataac tgctgctgtt
    37321 gcgactcgta ttcctgttct tgttggatca acttgccaaa ataagcttcc tgttcttgtt
    37381 tttgatgaat tttttcttgc agctctttcc attcatttaa tttttgattg attagtaatt
    37441 tttggccttt tttcttgaat aattgttgtg ctttctgata atattcttgc cgcttttgga
    37501 atacttgaga actgcccgtg atgcctaaag aaatcagcgc atcatgtagc tctttttctc
    37561 tcaaatgatc tagttccatc aactgctctt gctggaaagt aaatacctgc tggaataact
    37621 ctttcgtcaa aggtgcgatc agtttttcta gtaactcatc acttcccact tgatccccca
    37681 gccacacctt cgattttccg cgattttgct gcttgtagcg ctcgacagca aattcaccat
    37741 atacaggatg tttaagccat attttgccgc cataggctga gccgtctttt ggtgtataat
    37801 cctttttttt acggcctttt gaagggaaac caaataatat tgcttgaata aattgataaa
    37861 tcgttgattt tcctgcttcg tttcccccat agatcagctg attgcctgaa taaaagtcaa
    37921 aacttttttg acgccatttt ccaaaagcag caatctcgat ccttaaaatc ttcattcctg
    37981 atcctcctca aataaaaaat cttgattaag cagctgcttg gctttttcaa gtgtttcttc
    38041 ttgatattcc ggcagttcgc tcaatattct agccgcttct tgatgactat atacttcatt
    38101 taaaatttgt tggaaatctt gaggtgtctg atagtattga aataactgtt ccatcaattt
    38161 agcagaagca gcaagtgggg tcttcgtata aaaagtattc tctatgagag aaatacgcca
    38221 aataaagaaa tcatttatcc tatctgagaa ttcctctaac aaatagtcta atagctctcc
    38281 tgattgtatc cgttccaaaa catctgtatt gagatggtca taatctttta attttatctc
    38341 aattaaacta aatccatcct ctacttttga aagctgctga cgaatgtgaa caatgacctc
    38401 ttttgtcgtt cttgctttcc tcaatgatat ttcctttgtc ttccaataga cctctgctac
    38461 tttaatcgga tgtatttggc aacttccttt acttaattca actagtagaa tagatgtaga
    38521 agcttgttcc tttttagtat gaccttgagg cgcacctgga taaacaattg tttgcttttc
    38581 atttaagacc gttggcacat ggatatgccc taaagcccaa taatcgtatc ccttttcttg
    38641 catctttgag ggttggaaag gagcatagtt tcctttttga gagacgccag gttccccgtg
    38701 gtacaaccca atatggtaat ctgtgagttc tctagaagga aattccatca ctttgtcttt
    38761 ttgaatccac ggttgacgat aactaaatcc actgagagag acagtttctc cactttttat
    38821 cgttattttt tttgtctcta ccgtttcgga tgtaaataaa tggacatttt taggaaagga
    38881 gaaccaatac cgctcttttt gatagaaatc atgatttccg aatattataa atacaggtat
    38941 atggttcttt tctaaccgtt tcatctgttc tgaaaaatgt ttctgtattt ttaatgaagg
    39001 acgattttga tgaaaattat ctcctgcaag taaaacaaaa tcaacgttat ttattattgc
    39061 ctgttcaatg atatttgata aaacggtcag attcgtcttc aatagttttt cctgtacagt
    39121 tttatcaaga gttgtcagtc cttcaaatga ccgatccata tgtaaatctg ccgcatggat
    39181 aaatttcacc attaaaaatt tcctcctttt tttcttttat cataccattt tcaatgtttt
    39241 ttttcatcaa taaccgaaaa tttgttcgta ttatgcaaag aaattacgat aaatggccga
    39301 cttcaaaaaa taagtttatt cgaaaaaaag attgtgacat gatttttgtc acaatcctaa
    39361 atatctatgt tatattcgct tcgtttgaca ctgaagaagt actatttgta ctggaattta
    39421 tgctccattt ttccgtattt agaattctat gttctatact accttaatta gtttatatgg
    39481 taagaattct ttcaaatgct tgagatactc cgtctttatc atttgaaaca gtgacataat
    39541 cagctttttt catgatttct tgatgactat ttcccattgc aaaactaata tctgcacttt
    39601 caaacataga taagtcattt aaactatctc cgatcgcgat tctcaaaggt ttctttaaat
    39661 tgtagattga ttccaaacga tcaaacacca cacctttaga tacattttga ggtacgattt
    39721 caatatttac aggtgccgat tcagtaacag acacatttag catctttttc aaatggttaa
    39781 atatattagc tttcaattga cgattaggaa taaaaataaa aaatttgtaa atgctgatag
    39841 catccttttc tataatttcc tcaatattat tataagggat tgaacaaata gatttttcat
    39901 actcaagctc atagtttagc tctttgatag aaaattcact ccctacctcg tgaaaaattt
    39961 ttttcatttt atttttataa ttctgagatt cataacttcc gagatttgta tatattttgg
    40021 ttggaaattt cgataggaca gaatcaattt tttttacatc atttttatcg aaggaagact
    40081 caaatatctt tttttctcca tcaaatccaa cagaaccatt taaccctact gctggtatcc
    40141 ttaaaccaga ttttttgaga atattattaa tgtcttctac ttctcttcca gaacaaatga
    40201 atggaaaaca gtcttccggt aaatgtttca ttgttcttgc atttttttga ctaatgctct
    40261 ggtcagaatt caaaagagta ccgtctaaat ctataccaat tagtttcatt tcatccatgt
    40321 ctcctatacg ttgattataa attcactgct tattgtaagt ctaaatttct cacaaaacat
    40381 atactaatta tacctaattt aagtaagtac aaacatcatt ttaagaatag gaaaggacat
    40441 ttatatacta ttctctgtat ttttgattta taaataaata tgaattgaat ccaaatcagc
    40501 tatattacta ttttataata accaactata aaaaacgaag ttaggtgaaa aactaacttc
    40561 gatattcata aaagactcta ttagttcaac ttagcaaaag atttaaacaa aaaaagatga
    40621 actatttcta gttcatcttt ttgcctt
//
LOCUS       DACIFS010000020        39854 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.020, whole genome shotgun sequence.
ACCESSION   DACIFS010000020 DACIFS010000000
VERSION     DACIFS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.020"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            1..369
                     /locus_tag="IYA83_001061"
                     /pseudo
     CDS             1..369
                     /locus_tag="IYA83_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(495..1574)
                     /locus_tag="IYA83_001062"
     CDS             complement(495..1574)
                     /locus_tag="IYA83_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ8586510.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1677..2117)
                     /locus_tag="IYA83_001063"
     CDS             complement(1677..2117)
                     /locus_tag="IYA83_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8586511.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2174..2584)
                     /locus_tag="IYA83_001064"
     CDS             complement(2174..2584)
                     /locus_tag="IYA83_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ8586512.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2764..3198)
                     /locus_tag="IYA83_001065"
     CDS             complement(2764..3198)
                     /locus_tag="IYA83_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ8586513.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(3298..4146)
                     /locus_tag="IYA83_001066"
     CDS             complement(3298..4146)
                     /locus_tag="IYA83_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8586514.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4535..6301
                     /locus_tag="IYA83_001067"
     CDS             4535..6301
                     /locus_tag="IYA83_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ8586515.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6403..7662
                     /locus_tag="IYA83_001068"
     CDS             6403..7662
                     /locus_tag="IYA83_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ8586516.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7809..9110
                     /locus_tag="IYA83_001069"
     CDS             7809..9110
                     /locus_tag="IYA83_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8586517.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9113..9964
                     /locus_tag="IYA83_001070"
     CDS             9113..9964
                     /locus_tag="IYA83_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8586518.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9977..10792
                     /locus_tag="IYA83_001071"
     CDS             9977..10792
                     /locus_tag="IYA83_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ8586519.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10943..12109
                     /locus_tag="IYA83_001072"
     CDS             10943..12109
                     /locus_tag="IYA83_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8586520.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12339..12986
                     /locus_tag="IYA83_001073"
     CDS             12339..12986
                     /locus_tag="IYA83_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ8586521.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSETSTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13070..14014
                     /locus_tag="IYA83_001074"
     CDS             13070..14014
                     /locus_tag="IYA83_001074"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ8586522.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14149..15600
                     /locus_tag="IYA83_001075"
     CDS             14149..15600
                     /locus_tag="IYA83_001075"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ8586523.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15621..16457
                     /locus_tag="IYA83_001076"
     CDS             15621..16457
                     /locus_tag="IYA83_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ8586524.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16457..17116
                     /locus_tag="IYA83_001077"
     CDS             16457..17116
                     /locus_tag="IYA83_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ8586525.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            17242..17931
                     /locus_tag="IYA83_001078"
     CDS             17242..17931
                     /locus_tag="IYA83_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586526.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17937..18893
                     /locus_tag="IYA83_001079"
     CDS             17937..18893
                     /locus_tag="IYA83_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ8586527.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18937..19449
                     /locus_tag="IYA83_001080"
     CDS             18937..19449
                     /locus_tag="IYA83_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586528.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19705..20253)
                     /locus_tag="IYA83_001081"
     CDS             complement(19705..20253)
                     /locus_tag="IYA83_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8586529.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20342..21523)
                     /locus_tag="IYA83_001082"
     CDS             complement(20342..21523)
                     /locus_tag="IYA83_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ8586530.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21759..23267)
                     /locus_tag="IYA83_001083"
     CDS             complement(21759..23267)
                     /locus_tag="IYA83_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ8586531.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23426..23617
                     /locus_tag="IYA83_001084"
     CDS             23426..23617
                     /locus_tag="IYA83_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586532.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23820..24737
                     /locus_tag="IYA83_001085"
     CDS             23820..24737
                     /locus_tag="IYA83_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ8586533.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24730..25803
                     /locus_tag="IYA83_001086"
     CDS             24730..25803
                     /locus_tag="IYA83_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586534.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25951..26469
                     /locus_tag="IYA83_001087"
     CDS             25951..26469
                     /locus_tag="IYA83_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586535.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26450..26842
                     /locus_tag="IYA83_001088"
     CDS             26450..26842
                     /locus_tag="IYA83_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ8586536.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26918..27853
                     /locus_tag="IYA83_001089"
     CDS             26918..27853
                     /locus_tag="IYA83_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ8586537.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            27986..28765
                     /locus_tag="IYA83_001090"
     CDS             27986..28765
                     /locus_tag="IYA83_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ8586538.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28758..29075
                     /locus_tag="IYA83_001091"
     CDS             28758..29075
                     /locus_tag="IYA83_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ8586539.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29319..30929
                     /locus_tag="IYA83_001092"
     CDS             29319..30929
                     /locus_tag="IYA83_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ8586540.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31086..31934
                     /locus_tag="IYA83_001093"
     CDS             31086..31934
                     /locus_tag="IYA83_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ8586541.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            32165..32644
                     /locus_tag="IYA83_001094"
     CDS             32165..32644
                     /locus_tag="IYA83_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586542.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            32677..34905
                     /locus_tag="IYA83_001095"
     CDS             32677..34905
                     /locus_tag="IYA83_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ8586543.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35034..36677
                     /locus_tag="IYA83_001096"
     CDS             35034..36677
                     /locus_tag="IYA83_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ8586544.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36750..37295)
                     /locus_tag="IYA83_001097"
     CDS             complement(36750..37295)
                     /locus_tag="IYA83_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ8586545.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(37318..37803)
                     /locus_tag="IYA83_001098"
     CDS             complement(37318..37803)
                     /locus_tag="IYA83_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ8586546.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(37793..38605)
                     /locus_tag="IYA83_001099"
     CDS             complement(37793..38605)
                     /locus_tag="IYA83_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ8586547.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(38744..>39854)
                     /locus_tag="IYA83_001100"
     CDS             complement(38744..>39854)
                     /locus_tag="IYA83_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ8586548.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13176 a         7200 c         7808 g        11670 t
ORIGIN      
        1 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
       61 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
      121 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
      181 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
      241 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
      301 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
      361 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
      421 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
      481 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
      541 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
      601 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
      661 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
      721 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
      781 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
      841 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
      901 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
      961 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     1021 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     1081 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     1141 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     1201 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     1261 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     1321 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     1381 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     1441 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     1501 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     1561 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     1621 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     1681 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     1741 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     1801 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     1861 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     1921 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     1981 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     2041 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     2101 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     2161 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     2221 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     2281 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     2341 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     2401 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     2461 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     2521 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     2581 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     2641 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     2701 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     2761 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     2821 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     2881 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     2941 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     3001 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     3061 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     3121 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     3181 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     3241 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     3301 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     3361 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     3421 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     3481 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     3541 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     3601 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     3661 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     3721 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     3781 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     3841 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     3901 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     3961 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     4021 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     4081 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     4141 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     4201 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtctaaatta ttatgtttca
     4261 ttcttctaga atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg
     4321 gagaaattga tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa
     4381 cttttaaaag ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa
     4441 aaaactgtta ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt
     4501 gatgacgtca tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg
     4561 accggaaagt gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac
     4621 taggaaaaat gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac
     4681 tgaaaaatgg tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga
     4741 ttattgggaa attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt
     4801 tggggaagat aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa
     4861 tgtgattact gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt
     4921 cactattcca gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc
     4981 aaacggggac aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga
     5041 ccgagaagac tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac
     5101 ggaccttggc atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa
     5161 atacgatacg attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa
     5221 aaacttgatt gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa
     5281 tcatatgggt gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat
     5341 ctataaagac tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt
     5401 accagaaact gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa
     5461 actaaataca gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat
     5521 aaaagaattc gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt
     5581 ttggaaaaaa ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga
     5641 gatttggcat tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta
     5701 tgcatttaca gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat
     5761 ggtcagtgga atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt
     5821 caacttatta gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga
     5881 attgatgaag tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta
     5941 cgggacagaa atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg
     6001 ggagaaggag gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag
     6061 gaaacagttg tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg
     6121 tgaagataag ctctatttca cacggaaact agagggacag atcatctatg tttactttaa
     6181 tcagtcaaaa gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg
     6241 tcagttgtta gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg
     6301 aaaactgtta ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa
     6361 taagggtgta acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg
     6421 aaacagttgg gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc
     6481 aatggtaact cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt
     6541 gattctggat ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat
     6601 gtgaaaatca agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat
     6661 gggcctgctg gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta
     6721 ggacagcaag gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca
     6781 gacaaagccc aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc
     6841 cttgttttgt actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg
     6901 gagaatctat ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga
     6961 tttttagcta aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc
     7021 tatgtatttg gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct
     7081 gtagaaggaa tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa
     7141 gacataaaaa gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca
     7201 atcattgacg gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta
     7261 gccaaaattc ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga
     7321 tgggtagtca gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta
     7381 acgaatcagg aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa
     7441 caagctaggg aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa
     7501 tatgaaacag ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca
     7561 gaaaacttga tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa
     7621 gcagtaaaaa caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc
     7681 atttttataa aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat
     7741 tatcttgctc ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg
     7801 gcattcagat gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt
     7861 cacttattcc agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt
     7921 tcgccatatt cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag
     7981 gattggttac tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag
     8041 gcactttgca attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag
     8101 acgcttatcg agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg
     8161 aagtcataca taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc
     8221 ttctcatgat ttttacgatc attttcccag tcctagttac attgttcact gcatttacta
     8281 attatgactt caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact
     8341 tttttagtat cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga
     8401 ccgtgatttg gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg
     8461 ttgttgctca ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac
     8521 catgggcggt cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg
     8581 taggtgccat caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca
     8641 gtgcaatctc ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg
     8701 gctggttagg atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag
     8761 aagacttata tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca
     8821 tcacgcttcc tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga
     8881 atttcaataa tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg
     8941 gaggagctgg ttctactgat attcttattt cctggatcta taaacttacg actggaactt
     9001 ctccgcagta ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg
     9061 tgtcattgct ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa
     9121 atttcattcg agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct
     9181 tttaatactg tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa
     9241 gtcaggaaat attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt
     9301 tactcggcta ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc
     9361 tgtcctaaca atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg
     9421 ctatcgtttt atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc
     9481 gactatggca gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa
     9541 gtactggttc ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat
     9601 gaaaggatac tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc
     9661 agggcatttc aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt
     9721 tcaagcatta tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct
     9781 tcgaacacct gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac
     9841 aaaccaaaga gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct
     9901 attattcttc tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg
     9961 ataggagcag agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc
    10021 aactcaagtg tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt
    10081 atttttagtt tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc
    10141 gattccatta gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa
    10201 attttctgaa cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt
    10261 gacccctgaa attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac
    10321 ttttattgat tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg
    10381 ccgctatacg atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga
    10441 tttccaacga tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc
    10501 ttatagttta agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc
    10561 ttcctttttc ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga
    10621 gagtgcaaat ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg
    10681 gtttatccac tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct
    10741 gcttttatgg gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta
    10801 agcgatttat gcgacaatga atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt
    10861 tctgaaaaaa ttaaacttct tagttgattc ttttcttttt tagtattaac gtaatagggt
    10921 agttttttta aggaggaaaa tcatgttaga gaaaacttta tacaatcaat tattttcacg
    10981 ttctttctcg ctccctgtcg aagtaaatta ttgggacggt acaacgaaac aatacggaga
    11041 tacggacact cctccccaaa tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat
    11101 tacaagtaac gcttctttgg cactaggaga agcctatatg gatcatcgaa tcgagatcga
    11161 gggaagtatc caagcattga tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg
    11221 aaaagattat ctaaaatggc tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga
    11281 agatatccat gcgcattatg acttaggtaa cgacttttat aaactttggc tagatccaac
    11341 tttgacttat tcttgtgctt atttcaatac acctgaagat acgttggaac aagcacaaat
    11401 caataaagtc catcatatct tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga
    11461 tatcggttgt ggctggggaa cattgatgtt cactgctacc aaagagtata atgtcaaggc
    11521 aactgggatc acactaagtg aagaacaata cgatttgatt accaaaagaa taaaagaaga
    11581 acatttagaa gataaatgcc gtgtattatt aatggattac cgcgaactaa aaggcgaaac
    11641 gtttgatcat atcacaagtg taggtatgtt cgaacatgta ggttctgaaa atcttgaagg
    11701 ttatttcaaa gtggtaaaag atttattgaa accaaaagga acagctttga tccatggaat
    11761 cagccgacaa caaggcggtg cgacaaatgc ttggatcaat aaatatattt tccctggcgg
    11821 ttatgtccct ggtgttactg aattagttga taatattaca aaaaacgatt tgcaactgat
    11881 tgatttggaa agcttacgtc gtgactacca attgacatta gaacattgga caaagaattt
    11941 tcatcaagtc caagatcaag tcacgaaaga aatgggtgat tccttctatc gaatgtggga
    12001 tctatattta caagcttgtg cagcttcatt tgaatcaagt aatatcgatg tgattcaata
    12061 tttgcttgtt caacctggaa acaatgacat cccaatgcat cgtgcataat tgaggcaaaa
    12121 gtactgttta aagactattt aaaaagaagc taagttttat gatgctccag aagtgaactt
    12181 ttttgtctaa cttctaggat tgtctctaaa gcttagtttc tttttcttta tattttcata
    12241 ataaaagttt ggttctttaa tttttaaaca tgacagaaaa ttttttcttc gttatacttg
    12301 taatagaata ctattttata acaaagggga tcgaatcaat gaaaaaaact gttatttata
    12361 ctagtttatt tgcagctttg tttttagctg cttgtcaatc aaataccaat caaggcacat
    12421 ctgggaatac gtcttccagt agcgaacagc gctcttcaca ggtatcggac tcctcaacaa
    12481 aagcaagcac atcttcgtct agccagacaa aaagttctag tgaaacaagt actcagtcag
    12541 atatcaaagt gtctgcagct cgtgcgatcg aattgttcca agataaatac ccagatactg
    12601 caattactag tttggaatta gatagtgatt ggggatctta cttctacaag atagaaggtg
    12661 tcgatgatca aaaagaatac gaagcaagaa ttgatgctca aaatgaaaaa atggagacag
    12721 agaatccaga aactctagat cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc
    12781 tggatgttga taaattgatc acgattgagg aagcagcaaa aatcgccgtc gacaaagtag
    12841 gaagcggtac ggcaacagat tgggatttgg ataaagattt aggtactact tattgggaag
    12901 tcaaagtaaa agacggcaat caaagtacat cagtcaagat caatagccaa acaggcgaag
    12961 tactgtcaac tgaacaagat gattaagaat ttcttcggaa aacaagagtt gatttttctt
    13021 ttagtcatga tacactagac aaaataactt gtttgaagga gcgatttact tgagtttaga
    13081 aacgaccttt gccaatcata tctttgccaa tcctttgatg aatgcatctg gtgtccactg
    13141 catgactacc caggaattag atgaactggc tcattcagaa gcaggagcat ttatcactaa
    13201 gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca cgctattttg atgttccttt
    13261 agggagtatc aattctatgg gacttccaaa cttaggtttt tcttattatc tagagtatgc
    13321 tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg ttattttttt ccattgccgg
    13381 gatgagtgtc caggaaaact tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg
    13441 gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga aaaccacaac tagcttacga
    13501 ttttgaggcc acttatgaaa cactgaaaga agtattcagc atcttttcta agccgctagg
    13561 aatcaaattg cctccttatt ttgattttgc tcattttgat cagatggctg atatcttgaa
    13621 tcagtttcca ttgacctatg tcaatgctat caacagtgtg ggcaatggtt tgtatatcga
    13681 tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga
    13741 gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga
    13801 aatccaaatc attggtacag gtgggattcg gacaggacaa gatgcatttg aacatctgct
    13861 atgtggcgca agcatgttac agattggtac cgagcttcat aaagaagggc cggaaatttt
    13921 ttctagaatc ataaaagaat taacgcagat tatgtcagaa aaaggatata cgtcaattga
    13981 cgaatttaaa ggcaaattac gcaccatttc atagcaaaga gacgaaggtc atgattgatc
    14041 ttcgtctttt tttataaaaa acactcgtct gttcattcct gtgcaagcgt actcattgta
    14101 tgttagacga acttgtggga aaataacaga ggcaaaaagg aggataatat gaatgatgca
    14161 acagaattag caaaagagct taaagaaaaa gaatttagtt ttcaagaact agttacggat
    14221 atacagaaaa aaataaagaa gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca
    14281 ttcgagagta attcccacga gctattgatt ccccagtcag aaaaagggcc attggctggt
    14341 atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg gatggctagc tacttcagga
    14401 tctcgtttat ttgaaacaca ccgagcttct catacatcta attatgtgcg gcaagcagaa
    14461 gcgataggtc tagttccttt tggacaaacc aatgcgccag agtttggttt caaaaatatt
    14521 acagacccag tcatctatgg cccagcaaga aacccttgga accttgaatg tacaccgggc
    14581 ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa tcgttccttt agctggtgct
    14641 agtgatggcg gtggttctat tagaataccc gcttcgtttt gcggattgat tgggctgaaa
    14701 ccttcgcgag ggacgatgcc agttggtcca tatgcttgga gaggatggca aggagctgct
    14761 attgatttcg gcttgacagt ttctatgaga gacacggagg ctttatttta tgggatgaga
    14821 agtatccatt ccggtgcgcc atatcaagta tcgccagttg agtggcaaac acatccaaga
    14881 aaaaaacggt tgaagatagc tttttgtgtt gattcaccga tccactcaca gattagtgaa
    14941 gaggcagtac ttgctgtaaa aaaagctgta gatttccttg gaaaagaagg gcatgatttg
    15001 acagagattc cttacccact agatggtcgt aaactgatca cgtcttatta tcagatgaat
    15061 ggcgcagaaa cagcagcaat gatccgctcg attgaaacag gcatccagcg agctgtcaga
    15121 aaagaagaac ttgaacccat ttcttggact ttgttccaat atggtcagaa gctctctgcc
    15181 gctacttaca tcgattctct gcatgtatgg gatgaagcag caattgtcat ggaaaaccta
    15241 catcaagaat acgatctatt cctttcacca acgactgcat ttaccgcccc taaaatcagc
    15301 gaagatttgc aaagtgatgc gataagagca cagatggaac acatcagtga ttgttctgaa
    15361 gtagaaggtt tagacgttgt ttcttccatg tttgaggaga gcttgaagat cacaccttat
    15421 acacagttgg ctaatctgac tggacaacca gcaatcagct tgcctactca tttgacaaaa
    15481 gaaaacatgc ctctaggaat ccaatttatg gctgccagag gaagagaaga tttgctattt
    15541 caaatcggct atctatttga agcaaatcat cagttccatt taccaaatcc atggaaatag
    15601 cagaaacaag gagaagaaga atgaagaaaa aaagattgtt tgctctaata actagtgcct
    15661 tttttatttt aagcgcttgc ggtacagctg ccgctaatca ggaagataac agctggagta
    15721 atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg cacactgttt cccgcatctt
    15781 attataatga tcaaaatgaa ttagttggtt atgatgtaga tttggcaaaa gaggtggcta
    15841 aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt ggatggacaa atcacttctg
    15901 tttcaaaaag agaatcagat tttgctgcga acgattttgg tatcacaaaa gaaagagaaa
    15961 aaaaattctc cctttcagag actttcaaat attcatttga cagtatgatc gtacgaaaaa
    16021 aagatgattc aggaatccac tcacttgaag atcttaaagg gaaaaaagca gctggcgaac
    16081 ccaatactag ctatatgcgt ttagcggaaa aacacggagc taaattagtc acatatgaca
    16141 atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg tcgaacagat gttattctaa
    16201 atgattatta tctgcagaaa atggccgtat ctgctttacc agatatccct gtaaaaatac
    16261 tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg
    16321 ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa agaagatggg acgacaaaga
    16381 aaatatctga aaaatatttt caaactgatg tttcagtcaa accgaaagaa acgatggctg
    16441 agtcggtgag tgagtgatgt actttcctct aattgatttt tccctcatgt ggacttcact
    16501 tcctttcgta ctgcaaggct tggtatacac attagggatt ggcttcgtgt cgtttctttt
    16561 aggaaatctg gtagggttgc ttttgacagt tttaggtctt ttggattggc tgcctctgaa
    16621 tgtatttatt cgtttttatt tatcgttctt tagaggcatt cccgcactag tcctgctgtt
    16681 tctgttatat tttgggttac cttatcaatt aagtgcctta acggcttcag tcatctgctt
    16741 tacgattacc agcagtgcat ttattggtga aatctatcga ggatcactag caggcgtttc
    16801 ttcaggtcag tgggacgctg gtttagcttc tggtttttct ttctttgggg tattgaaata
    16861 tatcgttttg ccgcaagctt ttcgaatctc agtaccggca ctcagtaacg tagctatgga
    16921 tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc
    16981 aaagatcata ggcggtcgga cattcgatta tttttctctc tatattttag ttgcactgat
    17041 ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag tatttagaaa aacgttctat
    17101 gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg ctggcaccat ttctattttt
    17161 tgtttcttgt atattctgcc tagtatttta aaaccatttc atgtaaaatg taaatcgaaa
    17221 atatgttcgg aggtagaaaa catgaagacc ccgctaacca tcgaaataga atcctcgtta
    17281 taccaatatt gcatcgagca aggcggcttg gttgtagaag aagtcacgat gcctgatgac
    17341 caaggaattg tagatacgct tagctgcttg acaaaatttg atggaagccg agaatggcga
    17401 tgctatgagc tgaaagtgac aaaagcagat tttcgctcta cagccaaact gtcatttgtt
    17461 ggacattata attattttgt tttaccaagt aaactatatg aagaagtaaa aactgaaatc
    17521 cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc
    17581 tctggtacat ttctgattgc gaaaaaagca gcaaagaaag agcttgctgt accggaagaa
    17641 gctttgacaa accgcttcat ggcttcttta ttcagggaag taagaaaagc aaagcaaatg
    17701 gagcgaggcg taaaatattt tccaacagat caattgtaca aagaattgaa aagacgttca
    17761 gaaaatcaag atccattttc taacgaacga tattatgaac ggtttttaga ggaaatagct
    17821 agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat
    17881 ttgaggcaac aacagcagat ccgtcggttg cctacagagc cattggagtg aaaaatatgt
    17941 actatcctta tttaagagga aaacaatttg acctattagc attaaaagaa gcactgtcgc
    18001 gaggattgct ctccaataaa atccaaccag tgatcgaacc agtacgtgat tccgctacgc
    18061 ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcc aatcgctgtg atccaaaatc
    18121 cccaagtagg acaattcaaa ttattcgatc agcatgtgca cagttggaca gtcaaagaaa
    18181 atagctctgt cgtccctgca cagatcttaa cgccagaaaa tctcagtgaa gtactggatt
    18241 cacctccagc gtttcttgtt ttcgatggac agcactaccc aaaagataca gaggtgtgga
    18301 aacagttggc aggagttgat tcgaaatttt tgataccaga tactagtcgc tttcgcatct
    18361 ggctgcccga aaacaaaatt gtgatccgtg actcttttca aacgagaaag catgttgaaa
    18421 gttatgcaga taaaacagat gactttttta gtgatgacta tctttttttc cacgcagacg
    18481 gatatattgg attttctgat ttcacgatcg aaggtagccg ttatttcgat aaagggttcc
    18541 ctagccgagc gcttgctatc catctaacgt atattgatgc ttacggcaat atacgcgtga
    18601 aacattttgt ctctgacagt aacgactctg ctaaagacca agcattgaag tttcttgaag
    18661 caggagataa aatgaaaaaa tggatcatgc gtcaccatca tcaactcttg attacttcag
    18721 gaatgattga attattggct ctttaccagc aacaaaaatt ccctggactg ggagtgttga
    18781 aaaagtggtc acttatgcat catttggaac ttattagtca cctgctagat catccaaaag
    18841 attggcttaa atattattca aaagttcagg aactatatga agtgatccaa tgattagata
    18901 atgtaaatga aaaaggaata gaaaagagga aactggatgt atattcggca aacaaggaaa
    18961 gaagaagtag aagaagtggt agaaatcatt aatgccgcag cggatttcat tgcttcgcaa
    19021 ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt
    19081 gaaagaaaag aaagctatgt attaatagtg gaagatcaaa ttgctggaac agctgcatta
    19141 gtctcaagcg tggatcccgt ttacacaaaa atagatggta gttggaaaga atcaactggt
    19201 ccttatttat cgattcatcg tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca
    19261 aaaccgttct tagaacattt gttgacgatt gcagccagca aaaatatcca agatatccgt
    19321 atcgatactt atccagctaa tgaaccgatg gaaaagacca tctattctgc tggattcact
    19381 tatcaaggga tgattgaatt tccttttgaa cacggcgaaa gaaaagcata tcaaataatc
    19441 atcaattgac tataaaatca tctgagcaaa gataaagaca ttaacactac tgaaaaaaac
    19501 aatcatatga tagccaagag gttgtaacag aagtggccag cttcaaaaaa caagaaagaa
    19561 tttccgaaaa tgcttctcaa atttttgtgg aattttagcg tattttacga aatagctgct
    19621 ttctgaaact cccttcatcg agaggtcgtg acatagcttt tgtcacgacc tctcggctta
    19681 tcctacgagt attattcgat acatttatat caataatcct ttttttattt tatctgcagc
    19741 ttcttggaac gctccatcgt ttgacacgac taaaagtgag gggatagctt tcaaacgttt
    19801 caactgtgct aaatcttcta cataaaccgc cagtctggct gaaatctgtt cagatgaatc
    19861 tccacgtttt ttcaggcgcg attgcaatgt ttctttagag atcgtaataa agataggacg
    19921 gactaccgtc ccgaaatact catataaatg ccaaaaacca tttgctgtca aaacggcgta
    19981 acaatttcct actgtcagtt tctttttaat ttctgcttta ctggaaccat atttatgttg
    20041 agcatattgg tcccactctg caaactcatt tcccttgatc atctgattga atgtattgtc
    20101 agaaacaaaa taataatcct cttgatcttt ttccccaggt cttggtgatc gattcgtgta
    20161 agtaatgatc ttttgttccg gctgaaaaat agcttctgcg agtgaagttt ttccagaacc
    20221 agaaggaccg ataaatacat agcaataatt cattgtcatt tctcctttga tgattgaaca
    20281 agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa gtaactttgc ttttttgtat
    20341 attataccag caagccagtc acaatagcac ttaaaaagct gactaaagtc gctgttagca
    20401 aaattttcaa tccgtgtttt gcgacaagat ttcctttttc ttcatttaat cccttcatcg
    20461 caccagaaat gatcccgata gaagagaagt tggcaaatga aacgaggaag acagatataa
    20521 ttgctgttgt acgctcagta aaatggaaat taccatttgc taattctgtc atagcaacaa
    20581 attcatttgt aaccagttta gtcgccatga tactaccggc atccactgct tctttccatg
    20641 ggattccgct gatgaaggca agaggggcaa agatgtaacc taggatctct tggaaagtga
    20701 tgccgaatat cccgtggaag atagcattga tcatggcaat caaagcaaca aaaccgataa
    20761 gcatagcagc aaccgtgatc gccacatgga aaccatcaag aatgtattct cccaacattt
    20821 ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc atcttctttt tcttctaata
    20881 catagggatt tacaattgaa gccagaatat aaccgccaaa taagttcaaa acaagggcag
    20941 tgatcacata tttagaatcg atcagtgcca tataagatcc gacgatcgac atagaaactg
    21001 tagacatagc cgatacactc atggtgaaca aacgtttctc agataggaag ggtaattcct
    21061 tttttatgga aataaaaact tccgattgtc ctaaaatagc agaagctact ccattataag
    21121 attccaattt gcccatcccg ttgattttac ttaatcctag accaacagct ttcataaata
    21181 atggaagaat cttgatatag cgtagaatac cgataatagc agaaataaat acaattggca
    21241 tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc tacaccggca aaaacaaagt
    21301 caacaccttg cccagcaaaa gcaagaagat gatcaaacat ttttgccaat acagaaacaa
    21361 ccgctgtacc aaaagttgta cgtaataata cgaaaccaag aacaagctgt aaaacgaaca
    21421 tcacaattag tggtttcaat ttgattgctt ttttgttact gctgatcaaa aaagcaagcc
    21481 ctaatgtgac aaataaacca ataataccta ataaataact caaataatca ctcctattcc
    21541 aaattccgtt ttattttacc tgttttgcaa ataaaatact aatctttagt gaaattttca
    21601 ttactttttt agagtgaaac gcattcattt tccattagaa aataaagcga acaaaataaa
    21661 aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa aacgaaacta tcgattaagc
    21721 taatagtttc gttttcaaca tttataagta aactttttct attggtaata tagattttcg
    21781 gaagggtaga caggtgcgca ggtaatatcg attcctagtt ttttcaatgt ttgttcatca
    21841 tttttattga ccatcacagt agagtgggct tgtgctccct caagttcagc tagtttatca
    21901 taagcaactt gagcgattgg attcgttact gcactgattg ataaagcgat caagacttca
    21961 ttgatgctta gtgcatccaa agaggaatga agtgtctttg atttcaatcc ttgtattgtt
    22021 tcaagaacta atggtgacaa taaaggaatg tcatctgcga tatgggctaa atgttttact
    22081 gcatttaaga taactgccgc tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg
    22141 acttcatctg gtaattcgat tgcaatcaca gcagaaggat cttttgtagg actttgcgct
    22201 tgaatcttat ctgcatagtc atgggcaggg actaccacgt ttcggtcttc aggacgtaaa
    22261 ccgacttctt ccataatcag tttgattcga ttaactgctt cttcgtcgat caatcctttt
    22321 ttgaagtcgc attctgtttg gaaataacga cggatgattt cttgtttaga ggcttcttgg
    22381 acaacggtat catccacgat cccaaatcct acacgattca cacccatgtc agtaggagat
    22441 tgataaacag attctttacc agtaattttt tcaataatac gtttgattac aggaaatgtc
    22501 tcaacatccc gattatagtt cacagctact ttattgtagg catcaaagtg gaaggaatcg
    22561 atcatattga catcttttag atcgactgtg gctgcttcat aagcaatgtt cagcggatgt
    22621 ttcaacggaa cattccatac tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct
    22681 ttttggcttt catgatacag ttggttcaag gaagtagcga gctttccact tcctgggcca
    22741 ggagctgtca ctacaacgat tggtttagtt gtttcaatat actcattttt tccaaaacca
    22801 tcttctgaga cgattttttc tacgttgact ggatagtctt cgatttctga gtgtttatag
    22861 accttgatgt ttcgtctttc tagtttattg ataaagactt tcgtggcagg ctgtccacta
    22921 tagcgagtga taacgacact gttgattaat aagccataac cacgtaattc atcgattaac
    22981 cgcaagatat ccatatcata agtaatccca taatcaccac ggatcttatt acgttcaata
    23041 tctccagcat agacgcaaat cagtatttca gcttgatctt tcaatttttg cagcaatttg
    23101 attttcgcat cttcttcaaa tcctggaagt acacgtttcg catgcttgtc gtcaactagc
    23161 ttcccaccaa attctaaata taatttgtca tagtggttca ctcgttccaa aatatacgct
    23221 gactgctctt caatatattt ttctgaatca aaaccaattt ttttcactag gttccctcct
    23281 taagtactaa agcatttttt catttttttg aggattcgtc aactggaaga gcgaaattcc
    23341 tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag atagttattc attggtatga
    23401 tagtttttga taaggaaggt gctacatgaa aaaaagacag aaaaagaaaa acgcatataa
    23461 acactatatc cgttcgattt ttaccggtta tgaaagaatg cttgaagatc ccgaattgga
    23521 acaattgacg tttacttatc taaacgaaga aacgcagcta actcgtgatg agcatcagcg
    23581 cattcatttc acaacaagag atctgcctag taaataaaaa ataagataaa ggtcatcgga
    23641 ttcgattctg tcctatcaga tggaaagact agttgatcga aaaaaaggct gtaccaaatt
    23701 ttggtgcagc ctgttttatg gagcattgcc cgctcttttt tataacaatt ttttacagaa
    23761 aatggtacta ttgaagtatt aataagtgaa aaacagatga gtaagaggag gcaagtagta
    23821 tgaaagagcc agatacaaat gcaaattatg agccagacga actagaggag acgaatgcgg
    23881 atcaggatac aggacaagaa tcgtcttctt tttacgatat caaagaaaaa gcaagcatga
    23941 atctttccca attattagaa gacttacagg cttttgaaaa agctgtccgc gaagaagata
    24001 tccctgagat ctaccgttta tacaatggcc gattgaatga agaattaaaa agaagctcaa
    24061 atgaaaatca cgagatcgat caattgctga tgcgtaagat ccatgatcgt tttttacagg
    24121 aatttccttt tatggaacaa gtagaaaaca tctcgccaac gatgagctat tacaaaatcg
    24181 gcacttatta ccatgatcgg cccacggtcg gtattgatgc aagtctgcca gaaatttttg
    24241 ttttgcctaa gatcgatgaa gagtgggaga aatattccca accggagtca acggactatg
    24301 acaaaaaaat caatgagcta gacgcaaagg tgatcactgc tcagacagaa attgaacgtt
    24361 tagatgaaca aataaaagaa atcaatcgac aaatgactga tttgaacgac aataaaggat
    24421 tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt agagaagaag aagcaagtac
    24481 tatctaatga aaaattaggc tggcttcctt atattgagac gcctgaaaca atccagcagc
    24541 aaaaagaagc attgaagcaa gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa
    24601 aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga gggattcggt caagcaatcc
    24661 atgaattctt gatgaattat cttggcaatg agaatcctaa taatcaatct aatgaaggag
    24721 gttccgagta tgaatgaaca cgatgaagca gaatggacaa aaaatgaaga acaaacagaa
    24781 gatcatgaag aaacgaacac ttcattggaa gaacaaacaa gtaatcctct ggatgacaca
    24841 gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg
    24901 aatacgaata ctgcagaaaa cacccatgct tacgaaataa ctgaaaatcc cgggtattta
    24961 gaaggaaaag agcaagatca tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa
    25021 ttagcggatt tacgtatgag aaaagatttg atcgaatcag attttccaca attattagaa
    25081 tcatatgatc gttttatatt gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg
    25141 atcgaatact tcacgtatga attactgaaa ccgttgaatg atattggtct ggaacggtca
    25201 aattcagctt atgacacatg gaaaacaaga catttttcga tcaattatac gttgaatgga
    25261 caaaacgaat tagtcttttc ctttgtatta ccgaccatcg atcaacgata ccaagtggaa
    25321 tcgatgagtc tcctaaacgt atcaccagag acaatggaaa tcaatgtcca agatgaccgg
    25381 gtgctttctt tgatccgaca ctggtcagtg gatcgagtat tctctgcagg gcagatcacg
    25441 atattcaatc acaaattgaa tcaattatta gcccatgcaa gaacacttgg ttttaccgtt
    25501 aatcagacat tactagacaa tacgaagcca cttcgtttga atcttcagag cgaatttgaa
    25561 ttgacgaatg aagtattgga tgatattttt atcgtggcaa tgaaaaatcc atcctacgat
    25621 atggaaaaga tcaacgaaac tacttatcaa gtacgattag ataaaggaca gtctttaacg
    25681 ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct catctggaga ctacccacga
    25741 tctgtcattg atttctttat caattacgaa ttcttggtcc cattaatggt gagaaagtcc
    25801 tgattggata agttggatta ccagatgtca ttcagataat tctgttccaa aggacttgcg
    25861 gcagttatcc agaataattg tcacaagtct tttctgtatg tactaaatgg acgtgaataa
    25921 cttgctgatg aaggagggat ttcattagga atgtccacaa ataaaatagc acagcttagc
    25981 ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc aatttattcc gaatttccag
    26041 ccaatgactg ctattttttt atttatagta ctttacttaa gcttaaaaga tgcgttaatt
    26101 gtcatgagtc tttctattgc gatcagcagt ttttattttg gtgttggacc atgggtcatc
    26161 ggccaatgga tcagctatac tgttgttctg agtttgtttt caatcgtctg cttgagaaaa
    26221 acagtgcaaa ggaatctatg gttaaaggga gtctgttttt ttctggcggg catggtttac
    26281 ggaataggta tgaccgtttt tgatacgctg ttatatcgtt tgcctcaacc ttggatctat
    26341 taccttcaag gggtgtcgtt tgatttgatg cacagtatag gcaatgttgt gttctttctt
    26401 gtttttctgc cagtcgtcaa acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat
    26461 aatttatagt ttgcttgcaa tgagtgtcag tatactcatt ttgacaagtt gcagtacaac
    26521 ctatacacaa tcggcttcca accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat
    26581 cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga
    26641 tgatttactg agtattttga aacgaaattt tgaagtcaaa gaagataacg gcttcatcac
    26701 ttcgcttgaa gggcactcac aggatgagaa aaatcaactc tattggatgt ttactgtgga
    26761 cgggaaaagt gctactactg gtgccaaaga gatcaaattg aatgatggac aatcggtcat
    26821 tttcaatttg tctaaactat aagtttcata aaaaactgtt aaagggcgaa tattgccata
    26881 attcagtgag ttcagttgat gaaagaagga aaataacatg gaaaatttta aaaaaagcgg
    26941 aatgttttcc gtaattgctg ctctaggggc taatattcta gttgctatca gcaaatttgt
    27001 cggctatgca atcagtggca gtgccgccat gctaaacgaa agcatccaca gtatcgttga
    27061 ttgcagcaat cagattttgc tgttattcgg tgataaacaa gccaataaag gacagagcga
    27121 gttgcatcaa tttggtgaag gacgagcaaa atactttttc agtacaatcg tcgcaatgat
    27181 gcttttcttc ggcggtgggg cacttggggt gatggaagct tttgaaaagt tgacgcatcc
    27241 atcccatgaa gtgggtaatc cgatcattgt tatagcaata ctgttatttg gtttggtaat
    27301 cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact
    27361 ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa atactgatta tttttacgga
    27421 agacttgtgt gctgtaatcg gtttggtctt ggctttagca ggaacaatat tgacttatgc
    27481 aaccgggatt gctgcatttg atgcgatcag cggattgctg atcggtttct tgctgatggg
    27541 agccgccatt ttcctagcaa aagaatttta tagtctgatc atcggtgaaa gcgtgacagc
    27601 aagcgattta tcaaaaatca agttagcttt tgaacgtcct gagatcagtc ggctgatcga
    27661 tataaaaaca gttcatttaa gtccaacgga gattttagtt gcagcaaaag cagatatcgt
    27721 tggtaacaaa gagaatgaag cttactcggt catcaatgat attgaaaaaa atatgcgtca
    27781 gtctcttcct gacaagaaga tgtatatata cattgaaaca gataaatatg acccaaatta
    27841 ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa atgagaaaaa tataatcttg
    27901 tttttaaata aaacggctac aatttagcga atttatgtta caatgagtcc gctgtttatt
    27961 tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc
    28021 tttgcatgca gtgatgccac tttgtataag ctatatacca gtaggattag cttgcggagt
    28081 attgttgcaa aaagttggat ttgatcctct cttatcagga ctgttatcga ttctggtctt
    28141 ttccggtggc gcgcagtttt tagttgcatc tatgcttacg acgcaagcat catttgcaac
    28201 gacattattg atggttttct ttttagaatt acgctataca ttattaggag caagtcttgc
    28261 cggttttatg aaaaaagaaa agcggacgtt tttggctgtc ttttctcaat cactgaatga
    28321 tgaaaactat gcggtgaact acttaaagtt ttcaactgac ccttcctgga ataaaagaaa
    28381 ggctttatat gtcaattggt tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa
    28441 ttttttcggc tcggtgattc gagtagatgc tgaccttgtc cattttgctt tgacggccat
    28501 gttcattttc atgtttatca tgcagatgaa aagcgcacta ctgatcttta caggtttatt
    28561 ctcaggcgta ttaggggtcg tattcatggt attattccaa aatactttcg gattgattgc
    28621 agcaacagtc attgcttcat ttgccggatt tatgctggag aaggcattca aaaaagaaaa
    28681 agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag ccgctcttca acttcccgga
    28741 acaggaggcc aatcaccatg actaaactat atttgctact tttagttgct tgtctgttca
    28801 tcgtggcata tatcccgcgc ttgttcccga tgctttattt tactcatcga aaagtgccat
    28861 cctggttcag tgactggatg aaatatgtac cggtggcatt atttgcggcg ttagctttta
    28921 aagatgtttt cattactcat gaacatctgg atattgcttg gaatatcaaa atagctgcaa
    28981 tgattctagt agcaggcgtg gcttacaaaa cacgttcgat ggcgttgtca gttttgacag
    29041 gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt tttattttat taaaaacaga
    29101 gattgcgaca taagttgttc aaactgatct atccgaactg catttgcaac agtcaattct
    29161 ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt ggcttatgat gcaagctctt
    29221 ttttatttta ggaattagag agggatttgt ccttctatga ttttttgcta taatagggaa
    29281 gatgtttatt ttatgaaaga atagttgagg gaatttaaat gaataaaaaa ttgatgcaag
    29341 gaacattttg gctaaccttt gctaatcttc tatgtaaagt tttaggggta gtctatttga
    29401 ttccctggct aagtatgatg gggaacaatc aagatggtat gcttgctacg acattgtata
    29461 atgttggcta tcttccttat ggattgttcc tgatgcttgg tacagtcgga tttccaaatg
    29521 cgattgcaaa aaaagtagct gtagccacaa aagaaggaga taacgctgct tgtcggctga
    29581 tttttagaag tacgatcaat atcatgtttg tcatcggtat cgcctctgct gcactgatgt
    29641 atttatttgc tcctttacta gccggaatca gtccgattgc taacgttaat aatgggatac
    29701 tagctatcag aagtttatgc ccatctttga ttgctatccc aattcttagt gcaatgagag
    29761 gatatttcca aggaaaaaat gatctgcggc cttatggaac ttctttgatt atcgaacagg
    29821 ctgtgcgagt gatcgttatc ttggctggta cttattactt gcgcgtattg actgacggaa
    29881 ccatattaga agctgtattg atcagtacag tcgcttcttt cttcggtggg ttagctgcga
    29941 ttatccatat gtatatcgtc ggacgcagaa aagacttctt cgaacttaaa gattttttga
    30001 tttccagccg ttatttcgaa agagaaaacc gatctgcctc tgtctctatc attcgggaaa
    30061 cactaccgtt tatttttgtc ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa
    30121 cgatgaaacc actgcttcac tttttccgtc cagagatcgc agatcagcaa ctggaattct
    30181 tattcagccg tgcttctgtc aatccaaata aattgacatt gatcctgatc tccatggtag
    30241 gaacagtagc tataagcagc ttgccaatat taagcacgat gaagaaaaaa gatcggatac
    30301 aaatcgaaaa aacagtagga gatagttttt cgattgcgtt attgattctt cttccttcat
    30361 tgactggaat gtctttatta gctggaccac tatacacatt gttttttggc tatgatcctg
    30421 aaagtgtcgg atatttccaa atggctttgc tagcttcttt gtttttctct ttattcacga
    30481 tcctatctac gatgcttcaa tctttgaatc accatcgttt tgcgatccga ttaactgtag
    30541 aagcaattat cctgaaagtc atcttccaag taatcggatt aggattagtt ggcggatacg
    30601 ggatgagctt atcaaatggc ttagcattcg gtattgtctt tgttcgcggc tatcacttta
    30661 tgtgcaaaga ataccgaata gcacctttag caaagatcag caattttttc ttaaaaactt
    30721 tccgctccac cctgattatg cttgcagtat gttttgccgt tttctttttg ctgaatcagc
    30781 gattgtcaat ggaatcaaaa agctatgcaa tgatttattg tgtggctgta ggaagtattg
    30841 gcggcttagt atttttattt tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg
    30901 ttaaacatca tagcagaagc aaagaatagg ttaactaatc gttttttgct gtcacttgaa
    30961 aaacggcaac tcgtatgatt acgcttgtcg tttttctatt ttccaatttt agtttcatat
    31021 ctaatcttac ttatgatata gttataaggt ctggaaaaat tctttgaaac gaggaaatta
    31081 aacacatgaa acttgcagta gttacagata gttcggcata tctgccggaa cgtatcaaga
    31141 accatcccga cttattcgtt attccaatcc ctttgattat ggatgggaaa atttataacg
    31201 aaggaatcga tatcgaagcg gatgaatatt atggactttt aaaaaacagt aaagattttc
    31261 ctactacttc acaacctgtc gtaggggaag ttttgggttt atatgaggaa ttgaaagaga
    31321 aagggtatga cacgatttta agtatccatc tttcttcagg gatttctggc tttgtcaaca
    31381 cattatttgc gatgaaagat gacatcagcg gagtaacggt cattccttat gactctaaga
    31441 tcactagtat gccgatggga cacatggtag aagctgcctt agacttaaat gatgaaggtc
    31501 agtcccttga tgaaatactt gcacatatcg atcgaatccg agataatacc tacgcttatc
    31561 tgattgtaga tgatttgaac aatttagtac gaggaggacg tttgacaaat ggtgcagcgc
    31621 tgattggcag cttattaaaa atcaaaccga tcctgacatt ttctgaaggg aaaattgttt
    31681 tattcgaaaa aatccgttca agtaaaaaag catttgctcg agctgaagaa attattggta
    31741 gaagaaataa cgaaatacaa ccgccagtca aactttacgt gatacatgca aataatttag
    31801 aagtagcgat tgcggagaaa gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa
    31861 ttggtcattt cggaccagtc attggtacac atttaggtga aaaagccatc ggcattgcca
    31921 tttcagcgca ataattcctt ttattcaaaa aacataccaa aaatattcca acatcatctt
    31981 ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt ttatttctat
    32041 caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc gcatatagtt
    32101 tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat gaggagttaa
    32161 aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag aagactatga
    32221 taaagttgat ggaagaaatg cattaaatac ggatgcaaaa gggctttcac tgggccttgc
    32281 tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac atactggaga
    32341 aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt tagcgatctt
    32401 agctgcacaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat tttatgatcc
    32461 agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg tagaagtaga
    32521 taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac tacaaaaaga
    32581 cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg agctaggata
    32641 ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc actaacagaa
    32701 aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa aagccatcgg
    32761 acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca agatcctgaa
    32821 atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac acaattagcc
    32881 aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt tgcagatatg
    32941 gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa ggaacaagac
    33001 caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact gacactgatc
    33061 cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga gctagttcaa
    33121 aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc tgaaatcgtt
    33181 cgagcgattg aaaaaggata tttattagag attcaagagc cgactgtcat ccgagatgcc
    33241 tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca
    33301 ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg aaattaccaa
    33361 ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga aaaaatggat
    33421 cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa
    33481 gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc aaaagctaac
    33541 gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac ctataagcaa
    33601 ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc cactgattca
    33661 gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact ggaacttcag
    33721 gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa aggaagaacc
    33781 attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga aattctgcgc
    33841 ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa
    33901 gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag tagcgatatc
    33961 gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat gtctgcttct
    34021 gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc gcgacaaatc
    34081 atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc tttccaagga
    34141 aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga atcacttagc
    34201 cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa aggaaaatac
    34261 gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa ttttgacaaa
    34321 aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga tgaatctggg
    34381 tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa
    34441 ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga ccgttcgtca
    34501 agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca atggttaaat
    34561 gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc acttcgttta
    34621 gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa tattagtgac
    34681 ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga tatccagtct
    34741 gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat tggccaagac
    34801 aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac tgacttgaat
    34861 gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa taaagctcaa
    34921 cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa caaggatact
    34981 tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac gatatgatac
    35041 aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct agtttcaaag
    35101 atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt aattatttga
    35161 aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca tttgtcgata
    35221 atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg atggaagatt
    35281 ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg gatcttgtca
    35341 tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc
    35401 cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg aataattgga
    35461 catcgatttt cggcggttct gcttgggagt attctcatga aactggacag tattatctgc
    35521 atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg aaagaagaac
    35581 tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt ttagatgcga
    35641 tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct ttctcaccct
    35701 ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt tttgaagagt
    35761 acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga ccacaatggg
    35821 tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat atctggcagc
    35881 aagaaaaaga agggcaatta gatgtcctta aattgaaact tgcattaagc gcttggcaaa
    35941 cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat gttcctcgtg
    36001 ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca aaagcaatcg
    36061 ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag gaaatcggaa
    36121 tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg aaaagacttt
    36181 ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc attcgtgaaa
    36241 caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat gctggttttt
    36301 ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat gtagaacaac
    36361 aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc attagacaaa
    36421 ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat ccacaacttt
    36481 atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac ttaacagaat
    36541 cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa
    36601 attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa gctcggttat
    36661 ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt gcgaggaagt
    36721 ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc accgatatat
    36781 ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg
    36841 acaggtactt ggacatgttt gatatgcata ccgattgatg tgtcaccgat atcgataccg
    36901 gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa agcagcaaca
    36961 gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa attttttgct
    37021 tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc ttggacagct
    37081 agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca ttgaccaact
    37141 tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa
    37201 acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac tatttcagtc
    37261 aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct ttttcaatta
    37321 atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg caagtcccat
    37381 tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg gccaatcata
    37441 taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta ataaaagccc
    37501 taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc cttgtaaccc
    37561 gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat cgatcaatag
    37621 accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag cagcggtata
    37681 gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt
    37741 taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt gcttagtttt
    37801 catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta tgatcgttca
    37861 tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct actgctttgg
    37921 taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca caaccggccc
    37981 cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga tcaattgaat
    38041 agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc acaatgtttt
    38101 ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac gaagagggaa
    38161 gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg
    38221 gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct tcagtttctt
    38281 tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt atgaagtctc
    38341 gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca tctaatgaag
    38401 tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata gattccagca
    38461 taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt ccgaatagac
    38521 caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat ggatcagaac
    38581 cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt ataacgaaat
    38641 tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt aaaaaaacaa
    38701 aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac ttttgtgatt
    38761 ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc gggattgcat
    38821 caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc tcccaccatc
    38881 gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga gaatgatttt
    38941 tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa aagtcttcaa
    39001 tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc aaaaataggt
    39061 ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca taaacatttt
    39121 cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac agtaattgta
    39181 tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc cgagcgcttg
    39241 tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa ctctctagtg
    39301 tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga agaaataccg
    39361 catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa
    39421 aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta gcagtaaaac
    39481 gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga gcttccaaca
    39541 tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat cccatggaca
    39601 tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga aaagcgatta
    39661 atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc catcccatag
    39721 aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta tgggtcatat
    39781 aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt tgttccatta
    39841 aataaagaat agga
//
LOCUS       DACIFS010000021        39580 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.021, whole genome shotgun sequence.
ACCESSION   DACIFS010000021 DACIFS010000000
VERSION     DACIFS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.021"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            360..758
                     /locus_tag="IYA83_001101"
     CDS             360..758
                     /locus_tag="IYA83_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ8586549.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            995..1537
                     /locus_tag="IYA83_001102"
     CDS             995..1537
                     /locus_tag="IYA83_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ8586550.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1620..2171)
                     /gene="lepB"
                     /locus_tag="IYA83_001103"
     CDS             complement(1620..2171)
                     /gene="lepB"
                     /locus_tag="IYA83_001103"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ8586551.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2323..2550)
                     /locus_tag="IYA83_001104"
     CDS             complement(2323..2550)
                     /locus_tag="IYA83_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ8586552.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2547..3065)
                     /gene="msrA"
                     /locus_tag="IYA83_001105"
     CDS             complement(2547..3065)
                     /gene="msrA"
                     /locus_tag="IYA83_001105"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ8586553.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(3085..3798)
                     /locus_tag="IYA83_001106"
     CDS             complement(3085..3798)
                     /locus_tag="IYA83_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ8586554.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3801..4697)
                     /locus_tag="IYA83_001107"
     CDS             complement(3801..4697)
                     /locus_tag="IYA83_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ8586555.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4855..5697)
                     /locus_tag="IYA83_001108"
     CDS             complement(4855..5697)
                     /locus_tag="IYA83_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ8586556.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5829..6482
                     /locus_tag="IYA83_001109"
     CDS             5829..6482
                     /locus_tag="IYA83_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ8586557.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6545..7072)
                     /locus_tag="IYA83_001110"
     CDS             complement(6545..7072)
                     /locus_tag="IYA83_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ8586558.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(7092..8039)
                     /locus_tag="IYA83_001111"
     CDS             complement(7092..8039)
                     /locus_tag="IYA83_001111"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ8586559.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(8051..9937)
                     /locus_tag="IYA83_001112"
     CDS             complement(8051..9937)
                     /locus_tag="IYA83_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ8586560.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(10097..12175)
                     /locus_tag="IYA83_001113"
     CDS             complement(10097..12175)
                     /locus_tag="IYA83_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ8586561.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(12188..12463)
                     /locus_tag="IYA83_001114"
     CDS             complement(12188..12463)
                     /locus_tag="IYA83_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586562.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12825..13280)
                     /locus_tag="IYA83_001115"
     CDS             complement(12825..13280)
                     /locus_tag="IYA83_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586563.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13434..14642)
                     /locus_tag="IYA83_001116"
     CDS             complement(13434..14642)
                     /locus_tag="IYA83_001116"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ8586564.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14754..15086)
                     /locus_tag="IYA83_001117"
     CDS             complement(14754..15086)
                     /locus_tag="IYA83_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ8586565.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            15234..16109
                     /locus_tag="IYA83_001118"
     CDS             15234..16109
                     /locus_tag="IYA83_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8586566.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(16191..16772)
                     /locus_tag="IYA83_001119"
     CDS             complement(16191..16772)
                     /locus_tag="IYA83_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ8586567.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16778..18037)
                     /locus_tag="IYA83_001120"
     CDS             complement(16778..18037)
                     /locus_tag="IYA83_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ8586568.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(18039..19085)
                     /locus_tag="IYA83_001121"
     CDS             complement(18039..19085)
                     /locus_tag="IYA83_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586569.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19320..19595)
                     /locus_tag="IYA83_001122"
     CDS             complement(19320..19595)
                     /locus_tag="IYA83_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ8586570.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19838..21148)
                     /locus_tag="IYA83_001123"
     CDS             complement(19838..21148)
                     /locus_tag="IYA83_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ8586571.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21330..22559)
                     /gene="rpsA"
                     /locus_tag="IYA83_001124"
     CDS             complement(21330..22559)
                     /gene="rpsA"
                     /locus_tag="IYA83_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ8586572.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22694..23374)
                     /locus_tag="IYA83_001125"
     CDS             complement(22694..23374)
                     /locus_tag="IYA83_001125"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ8586573.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23423..24058)
                     /locus_tag="IYA83_001126"
     CDS             complement(23423..24058)
                     /locus_tag="IYA83_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ8586574.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(24124..25527)
                     /locus_tag="IYA83_001127"
     CDS             complement(24124..25527)
                     /locus_tag="IYA83_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ8586575.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25514..26545)
                     /locus_tag="IYA83_001128"
     CDS             complement(25514..26545)
                     /locus_tag="IYA83_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586576.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26576..26797
                     /locus_tag="IYA83_001129"
     CDS             26576..26797
                     /locus_tag="IYA83_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ8586577.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26962..27699)
                     /locus_tag="IYA83_001130"
     CDS             complement(26962..27699)
                     /locus_tag="IYA83_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ8586578.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27852..29192)
                     /locus_tag="IYA83_001131"
     CDS             complement(27852..29192)
                     /locus_tag="IYA83_001131"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ8586579.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29429..30952)
                     /locus_tag="IYA83_001132"
     CDS             complement(29429..30952)
                     /locus_tag="IYA83_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ8586580.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31331..31942)
                     /locus_tag="IYA83_001133"
     CDS             complement(31331..31942)
                     /locus_tag="IYA83_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ8586581.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(32065..32190)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(32272..32988)
                     /locus_tag="IYA83_001134"
     CDS             complement(32272..32988)
                     /locus_tag="IYA83_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ8586582.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32994..33563)
                     /gene="scpB"
                     /locus_tag="IYA83_001135"
     CDS             complement(32994..33563)
                     /gene="scpB"
                     /locus_tag="IYA83_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ8586583.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33547..34344)
                     /locus_tag="IYA83_001136"
     CDS             complement(33547..34344)
                     /locus_tag="IYA83_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ8586584.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34314..34685)
                     /locus_tag="IYA83_001137"
     CDS             complement(34314..34685)
                     /locus_tag="IYA83_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ8586585.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34704..35591)
                     /gene="xerD"
                     /locus_tag="IYA83_001138"
     CDS             complement(34704..35591)
                     /gene="xerD"
                     /locus_tag="IYA83_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ8586586.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35687..36160)
                     /locus_tag="IYA83_001139"
     CDS             complement(35687..36160)
                     /locus_tag="IYA83_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ8586587.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(36265..37128)
                     /locus_tag="IYA83_001140"
     CDS             complement(36265..37128)
                     /locus_tag="IYA83_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ8586588.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(37324..39423)
                     /locus_tag="IYA83_001141"
     CDS             complement(37324..39423)
                     /locus_tag="IYA83_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ8586589.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        10933 a         8228 c         6711 g        13708 t
ORIGIN      
        1 aatgcgcagt tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt
       61 catccgttgt aagatgggta taggtcattt gtactcactc cttataattc tttggacgga
      121 actattttga gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc
      181 gttataaaga aattccatct attagttaag tgaaagtgaa ccagtcctca ttctatcaca
      241 tcttcacttt tttgatgatg caaattttta ttttggtttc tatatatgaa tctaaatttt
      301 tgacagcgtt tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa
      361 tgaatagtat ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt
      421 atgaaaagct gggttttaaa aaaaatgaag aattttctac tgaagaagct ggttcaatgg
      481 tatgggatga cactttttgg ataatgcttc tctctcatga tttttataaa aaatttataa
      541 aagataaaga aatagctgat aataaaaaaa caagtggtgc tttgatttcg tttagtgttg
      601 aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa agaaaatggt ggtactttcc
      661 acgaagtaga tatgggaatg cctgaagaag aaatgttttc attagaagtc caagatctag
      721 acggaaatac attagaaccc gtatggatga agatgtaagc agataactaa tctgacgttt
      781 tttataacaa aaaaacaaag gcggatgata cgataagtat tctccgcctc tatttttttt
      841 attcgtacaa tctctttttt acagtttata aaagcagtct tctcaccctt tttttaattg
      901 ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca cattggtgat tttatggtat
      961 ccttttatta ttaactgaca aaggagagaa tattttgaac actttgattg caaaagacgt
     1021 ttttagattg cccgaaatga atgcaagatt gttgtcgacc tctgctcttt tattagccct
     1081 tgatgtcatc ttatacaaga tagcgattgg tcctgcttat tttcaaatca cattcggatt
     1141 tttaagtatg gcgcttatgg gttatctata tggaccgatc tgggctggtt tgcttgaaat
     1201 gttgtcgaac actttaaatt tcacgatatt tggttcagga acgtttcaag ctgcttttct
     1261 tatcacggct ttccttggtg gatttattaa tggcttattt ttatatcata aaaagataaa
     1321 cctgccttcc gtcatcgctg cccaattatt gattatgatc ccagtcagcc tttttatcaa
     1381 ttcttggctg atgtctgttt tgtttggtgc agatttcaaa gtaatttttc taggtagatt
     1441 catcaggaat atgatccaaa tccctattca aataatggtt gtttattcat ttatcaaaac
     1501 tttagaggct agaaaagtgt ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac
     1561 tgattttgga ttaaacaata aagcgtgcag tctcaacaga ctgcacgctt tattgtttct
     1621 cactggatcc attttatttc atcaagtgga taatagacaa atcttgcttt acctagaata
     1681 tcagttttat tgatcgtgcc aaacgaacga ctatcttttg aaactcttcg attatctcca
     1741 agtacaaaat aattatcttc cggaagcttg gattgtcccg ttagctcttg tagtgtaaag
     1801 tccgtcgtat aggatgcatt tgcatgatct gatttcaggt ttttagttag ataaggttcc
     1861 ttaactactt tcccattgat cttcaattga tcattttggt agctgacatt ttctcctgga
     1921 agaccgatta ctcgtttgat ataaatcgtg ccatctgcta attgaaaaac aacaatatca
     1981 aatcgtctga tctccgaaaa tttttccatc acaaccatat caccttgctt caacacgttt
     2041 tccattgaat tgccttccac tggtaccggg atgaataaaa atccccgcaa catgaacgca
     2101 aggaatacag aaacaagtaa gtatttgaat acaagccaga agcgattaat atagcgttgt
     2161 ttttttgtca atctctatca atccatccct ctttttttca ttctttcttt attataagaa
     2221 aaatatcaaa aaaatgcgac tagatagctg aaataaaaat aattctttca attaggctat
     2281 tggacaaaaa acttttttga tcataaagta caaaactatc tcttatatgt catgattatt
     2341 ttctaaatat ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt
     2401 cagttccaga taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc
     2461 tttaccggct tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt
     2521 cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag
     2581 gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga
     2641 tggtcttctt ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt
     2701 tcgaaacgtc cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct
     2761 tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa
     2821 gcgtcagttg gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat
     2881 gttggatcat agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt
     2941 tcataagtcg gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca
     3001 ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt
     3061 tccattatga ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt
     3121 gctttcagta ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt
     3181 atcttttggc aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc
     3241 tttaatatac atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc
     3301 atctgtattg acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac
     3361 atctttgatt ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt
     3421 tccgttttcc atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac
     3481 ttcgaaagta ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact
     3541 atctttttga tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga
     3601 attgatcgtt aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc
     3661 tggttcccga ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc
     3721 aagaaggata aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac
     3781 gtcagatctt gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc
     3841 tcgatcagct gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg
     3901 aaatgatctt cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta
     3961 gttgtttgat ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc
     4021 aaatcattga ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct
     4081 ttgttccaat tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat
     4141 ggattgtaga tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc
     4201 aatagttctg tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt
     4261 aacccgaata aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg
     4321 ataaaatcag ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag
     4381 atctggtcac ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta
     4441 taacggtctt ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct
     4501 cctactcctt cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt
     4561 ggatcttctc tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca
     4621 gctaaacctg catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt
     4681 cgtttcgtta atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa
     4741 ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa
     4801 gaaaaagaga ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc
     4861 tacataatac atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt
     4921 atgcagtaca gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg
     4981 cgccaattct tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt
     5041 cagttcattt ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt
     5101 tacagggatt aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga
     5161 cagcaaacca gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat
     5221 tccgatgtat aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac
     5281 tgttttacct tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc
     5341 agtggtatca ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc
     5401 tgcttctact gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc
     5461 ttctcctagc tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg
     5521 cagatttttt gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc
     5581 gggatatact gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc
     5641 gcgtacattt ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat
     5701 tttctctctc tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat
     5761 ttctattttt agtataacag acaaataatg atttttcatt aattacacct agtaaaggag
     5821 ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta
     5881 atgcagtcac ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag
     5941 taaaaggtgc ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga
     6001 tgatgattct tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa
     6061 aaaaggtgtt ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata
     6121 ctcctttttg tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct
     6181 ggttgtttgc gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag
     6241 tatcaaaagt atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg
     6301 ttcctcttta tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt
     6361 cttacacgat aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt
     6421 ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt
     6481 aataaaaaaa ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc
     6541 ttttttatat cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg
     6601 tttcaaattg atgcggataa cgattttttt catccatttc tccttgacta gtattgatca
     6661 gtgaccaatc agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga
     6721 tgactgtacg gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc
     6781 cagcgatgaa aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat
     6841 gcatgacatg cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac
     6901 gattcggcaa cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat
     6961 ttgcttctgt catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt
     7021 cttcttttcc aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat
     7081 cctttctgat tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct
     7141 aattggatat cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa
     7201 gtcggaaatg cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata
     7261 taaagatgag catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt
     7321 gcaattaggt gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca
     7381 tccccactgc gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac
     7441 atcgtatgac aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg
     7501 atcaatcttc tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg
     7561 ataaagccac cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga
     7621 tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc
     7681 tgctttttat attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc
     7741 atatctggtc cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa
     7801 atatggtttt tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc
     7861 aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt
     7921 tgcagatcaa accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct
     7981 tctttacgat gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc
     8041 ctttttcctc ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt
     8101 caagttgtgc ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat
     8161 ctccttgatg attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct
     8221 catcttcaat cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag
     8281 gttccttgct ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct
     8341 tcagtaaata actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata
     8401 ctaataactt atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag
     8461 cacctggaaa acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat
     8521 tggtcggttc atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata
     8581 accggcgttt ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa
     8641 ataaaaatcg ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt
     8701 cttcagcagc ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt
     8761 cttgtgtata atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg
     8821 gaattctgcc agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa
     8881 tacctagtct ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat
     8941 ttatacggta actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta
     9001 ggttcagctc taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga
     9061 aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag
     9121 ctaattcctg tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac
     9181 gttctgcttt ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa
     9241 atgaaagttc gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca
     9301 tcaatagtgc gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat
     9361 ctaaatggtt tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg
     9421 ccagactaac tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac
     9481 tgattcctaa tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt
     9541 tattcatttg ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt
     9601 cccctagttc gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc
     9661 cttgaaagac tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat
     9721 aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga
     9781 taccggcaat aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga
     9841 tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag
     9901 tttttttgag gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt
     9961 gtatcataaa aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct
    10021 caaaaaaaga acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaaactt
    10081 gaaaattttt acctttttat tttattggac tcgttttttt tgactttgct cgaaacgcaa
    10141 atcggaaaga aaagttatca gatggtttgt tcatcgtata agattctaac aaattgatat
    10201 tcctgcgata gctgttcgca gaaaacgtcg tgattttttc agcaacttcg tattcatcaa
    10261 tgatttttct ttcaagataa taacctctta gcattttttt cgtaaacaga ctatcttgct
    10321 ggatcatcca cgaagaaatc aaattccccg agttcatttt tattccttca agtttgcgtt
    10381 gctggataaa aaattgaacc atcgtttcat cataaatatc agtcaagtta tctaaacttt
    10441 caataatcaa ctgagtattt ttttgatatg tctctctgat ttcttggata tcttcatcac
    10501 taataaatgt ctgacgatca gcatatcttc tttgccaaaa cattttcgga tggagaacga
    10561 ttcgcattaa cctacggaca acaatgaacc aaaagccgat aaaagacaaa atctggcttg
    10621 tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa acgataacca ttagaagaaa
    10681 gcttgcccga acgataactt tcatccaatc catcccgttc gatcgacaaa atcagtgctt
    10741 gtatttcttg tacttcttgc ttttcatcat cggttaaatc ttccaagtac agatcacgta
    10801 tccgttcttg ataattttct atcactgctt cagtggctaa aaattctttt gtattacgat
    10861 caatttcctt tatttctttt cgcaaaactt caacgacctc ttctaaaatc atcaaagcat
    10921 tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat aggcaagaca atgatcccta
    10981 tgaccaaagt cactaagatg acacccgccg tcagaaacaa aagcaaagac cgctggtaaa
    11041 acatcatatt attgattgaa aatggcaaaa taaaaatcgt agccaaactg actgttccct
    11101 ttacaccgcc aaatgttaag atcaagatct cgttcaactg ttttttgaac tttttactgc
    11161 catctttgaa tacataaaaa agactgatag aaatgaatct gatcacaaaa agcatgacag
    11221 agattaatac gataatcgct aataaaagac cgtttggata taaaccgtct ccccatactg
    11281 gcgagaacac ttgcgtcaat tcgattccta aaaatataaa tactaatgca ttcagtgtaa
    11341 aagcaatagt tgtccatgta ctgtgggaca agctcgacaa ttctgcatca aacacagaga
    11401 ttttcctaaa accagaagct tggagaatac ctgctgccac tgcagcaatg attccagata
    11461 catcaaatgc ctctgccaat acataggcta gaaaaggaag caaaagttcg atcagcagat
    11521 accctgtgac atcttgtgct gatgcttttt cgatcaaatg aattattttt tgtttgaacc
    11581 agacaagcaa aaagccgatc aaagctcccc caatactaga aactactaga ctcatccccg
    11641 cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa
    11701 caccagatgc atcattcaaa agaccttctc cttccaaaat atgcatcgcc tttttaggga
    11761 tattcactcg tccagaaaga gaactgactg ctaccgcatc tgtcggacct aacgctgctc
    11821 caaaagcaaa gcaggcagca agtgggacac ttggcaacag gaatgataac gtcgcaccta
    11881 ccccgacaag ggtcaaaatg actcctccaa atgccaagaa caaaattgtc ccgaaatttt
    11941 tgagaatcga agaaatatct gctttttctc cttctctaaa taacaatggt gcaataatca
    12001 taactaagaa caattcaggt tcaaaatcga ttgattgtcc ccactctgtc agacctaata
    12061 ccactccaag gaaaatttgg atcagtggag ctggaatcat tggcaaaatg cggctagcta
    12121 tgttcgataa ggtgatcgtt actgcaaata caataactaa ctcagcaaat tccatcgtcc
    12181 atccccttta ttttttattt tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa
    12241 ttttggtttc gactacttca acctgtgttt ggttcccctc taattcacac tgtacacgtt
    12301 ttgactccaa acgaagcaga tcatgtttca gacgttccaa ctgacgatac atcaatatat
    12361 ttcttttacg caacttacaa agtatttgac ggcggtcatc catcgaaagg gcgccatagc
    12421 gatcatgcaa cagttttttt tgttgttcaa aactctctct cattttccag atctcctata
    12481 cacaaacttc catcattata gcaaagttct ttttggatga aaaatatttc attgtttcat
    12541 cgtttattgc tagttagaaa tttccataaa tttttcaacg cctcattcta ttccaaatag
    12601 tatcagatat caagtaaaac ttaagaaaga taaaagtttc tgacaaaagg atgtttactt
    12661 tcccattcct gcctttcttg ttcttttatc acaacaaaaa aatggggctg cgacaaaaat
    12721 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
    12781 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
    12841 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
    12901 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
    12961 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
    13021 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
    13081 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
    13141 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
    13201 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
    13261 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
    13321 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
    13381 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
    13441 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
    13501 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
    13561 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
    13621 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
    13681 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
    13741 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
    13801 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
    13861 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
    13921 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
    13981 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
    14041 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
    14101 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
    14161 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
    14221 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
    14281 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
    14341 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
    14401 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
    14461 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
    14521 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
    14581 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
    14641 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
    14701 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
    14761 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
    14821 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
    14881 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
    14941 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
    15001 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
    15061 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
    15121 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
    15181 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
    15241 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
    15301 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
    15361 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
    15421 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
    15481 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
    15541 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
    15601 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
    15661 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
    15721 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
    15781 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
    15841 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
    15901 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
    15961 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
    16021 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
    16081 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
    16141 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
    16201 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
    16261 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
    16321 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
    16381 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
    16441 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
    16501 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
    16561 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
    16621 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
    16681 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
    16741 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
    16801 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
    16861 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
    16921 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
    16981 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
    17041 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
    17101 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
    17161 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
    17221 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
    17281 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
    17341 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
    17401 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
    17461 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
    17521 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
    17581 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
    17641 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
    17701 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
    17761 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
    17821 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
    17881 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
    17941 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
    18001 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
    18061 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
    18121 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
    18181 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
    18241 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
    18301 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
    18361 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
    18421 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
    18481 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
    18541 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
    18601 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
    18661 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
    18721 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
    18781 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
    18841 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
    18901 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
    18961 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
    19021 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
    19081 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
    19141 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
    19201 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
    19261 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
    19321 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
    19381 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
    19441 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
    19501 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
    19561 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
    19621 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
    19681 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
    19741 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
    19801 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
    19861 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
    19921 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
    19981 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
    20041 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
    20101 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
    20161 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
    20221 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
    20281 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
    20341 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
    20401 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
    20461 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
    20521 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
    20581 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
    20641 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
    20701 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
    20761 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
    20821 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
    20881 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
    20941 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
    21001 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
    21061 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
    21121 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
    21181 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
    21241 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
    21301 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
    21361 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
    21421 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
    21481 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
    21541 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
    21601 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
    21661 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
    21721 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
    21781 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
    21841 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
    21901 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
    21961 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
    22021 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
    22081 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
    22141 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
    22201 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
    22261 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
    22321 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
    22381 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
    22441 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
    22501 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
    22561 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
    22621 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
    22681 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    22741 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    22801 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    22861 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    22921 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    22981 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    23041 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    23101 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    23161 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    23221 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    23281 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    23341 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    23401 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    23461 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    23521 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    23581 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    23641 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    23701 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    23761 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    23821 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    23881 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    23941 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    24001 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    24061 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    24121 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    24181 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    24241 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    24301 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    24361 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    24421 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    24481 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    24541 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    24601 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    24661 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    24721 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    24781 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    24841 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    24901 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    24961 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    25021 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    25081 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    25141 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    25201 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    25261 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    25321 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    25381 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    25441 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    25501 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    25561 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    25621 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    25681 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    25741 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    25801 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    25861 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    25921 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    25981 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    26041 aaaacagctg caccaaactc tcatacgcag tatcaattaa aatttcacgt ggcaattggc
    26101 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    26161 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    26221 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    26281 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    26341 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    26401 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    26461 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    26521 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    26581 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    26641 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    26701 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    26761 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    26821 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    26881 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    26941 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    27001 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    27061 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    27121 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    27181 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    27241 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    27301 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    27361 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    27421 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    27481 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    27541 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    27601 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    27661 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    27721 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    27781 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    27841 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    27901 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    27961 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    28021 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    28081 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    28141 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    28201 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    28261 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    28321 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    28381 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    28441 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    28501 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    28561 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    28621 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    28681 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    28741 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    28801 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    28861 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    28921 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    28981 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    29041 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    29101 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    29161 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    29221 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    29281 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    29341 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    29401 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    29461 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    29521 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    29581 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    29641 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    29701 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    29761 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    29821 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    29881 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    29941 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    30001 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    30061 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    30121 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    30181 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    30241 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    30301 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    30361 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    30421 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    30481 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    30541 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    30601 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    30661 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    30721 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    30781 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    30841 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    30901 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    30961 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    31021 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    31081 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    31141 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    31201 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    31261 tacattattg catgtcttgt atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc
    31321 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    31381 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    31441 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    31501 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    31561 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    31621 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    31681 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    31741 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    31801 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    31861 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    31921 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    31981 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    32041 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    32101 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    32161 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    32221 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    32281 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    32341 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    32401 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    32461 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    32521 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    32581 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    32641 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    32701 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    32761 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    32821 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    32881 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    32941 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    33001 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    33061 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    33121 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    33181 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    33241 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    33301 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    33361 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    33421 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    33481 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    33541 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    33601 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    33661 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    33721 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    33781 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    33841 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    33901 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    33961 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    34021 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac
    34081 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    34141 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    34201 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    34261 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    34321 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    34381 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    34441 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    34501 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    34561 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    34621 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    34681 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    34741 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    34801 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    34861 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    34921 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    34981 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    35041 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    35101 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    35161 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    35221 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    35281 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    35341 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    35401 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    35461 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    35521 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    35581 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    35641 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    35701 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
    35761 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
    35821 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
    35881 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
    35941 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
    36001 tttttttcac taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa
    36061 gcacaagcag agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct
    36121 gttttgtttt tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt
    36181 ataataatta caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag
    36241 ttaagaaaaa catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt
    36301 ttcttgtgta atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt
    36361 gctgatacca aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg
    36421 ctgatctgca gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc
    36481 atatgctctt ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg
    36541 accttttatc tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc
    36601 aagatagaaa tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc
    36661 tatcacatct ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt
    36721 ttcatctgct aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa
    36781 gcgatcccct tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac
    36841 aaagtcttta tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt
    36901 aacgattcca aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt
    36961 ttcatgtttt tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc
    37021 ttctgatttg cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt
    37081 ttcatcaata ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct
    37141 ctatagtgaa aggttttctt aatacatttc tttgctatca taacataatg ttatctactt
    37201 gaatagctga gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata
    37261 ctcccctttt tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta
    37321 aaattaattg ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta
    37381 aaatggcaaa gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata
    37441 tagattataa tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc
    37501 aacgatatat ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca
    37561 cgtccatgct ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg
    37621 attcgctgag tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc
    37681 gaccgcagct tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg
    37741 ttcttcagct gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc
    37801 tttatcagtg agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc
    37861 tgtatttttc actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga
    37921 atccactact tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc
    37981 agcatattga tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt
    38041 atccgtttcg attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg
    38101 atcatcgacg atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt
    38161 agattggata tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact
    38221 ggataaatca ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg
    38281 atggtgatca tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg
    38341 tctttttgct tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca
    38401 gcttcctcca ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt
    38461 tttcttttgc gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc
    38521 agtatctggc acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc
    38581 ttctttttct atttttgctt caagatcagt accctctttg cttacagatt gattttcagc
    38641 atcggattca ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat
    38701 ttcttttttc tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt
    38761 aaatacttca gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt
    38821 acctgtggcg ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac
    38881 tttgttttcc tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc
    38941 ttttgtatct tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt
    39001 atcgacgatt tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc
    39061 ttgattcttt cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt
    39121 ctctatatta gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc
    39181 gaataattcc attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc
    39241 gtttaattct gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc
    39301 tgtatttgtt tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc
    39361 gcagtctgga cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt
    39421 catcacttag cgatcggctc ctttactagg tttacgctac catttaatct atcatattct
    39481 atacgtcacg ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt
    39541 cctatagaaa aaaaagcttg agactgcgta gtctcaagct
//
LOCUS       DACIFS010000022        38500 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.022, whole genome shotgun sequence.
ACCESSION   DACIFS010000022 DACIFS010000000
VERSION     DACIFS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.022"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(203..829)
                     /locus_tag="IYA83_001142"
     CDS             complement(203..829)
                     /locus_tag="IYA83_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586590.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1124..1459)
                     /locus_tag="IYA83_001143"
     CDS             complement(1124..1459)
                     /locus_tag="IYA83_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ8586591.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1446..1730)
                     /locus_tag="IYA83_001144"
     CDS             complement(1446..1730)
                     /locus_tag="IYA83_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ8586592.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1786..3309)
                     /locus_tag="IYA83_001145"
     CDS             complement(1786..3309)
                     /locus_tag="IYA83_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ8586593.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3302..4477)
                     /locus_tag="IYA83_001146"
     CDS             complement(3302..4477)
                     /locus_tag="IYA83_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ8586594.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4481..4666)
                     /locus_tag="IYA83_001147"
     CDS             complement(4481..4666)
                     /locus_tag="IYA83_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586595.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4632..6326)
                     /locus_tag="IYA83_001148"
     CDS             complement(4632..6326)
                     /locus_tag="IYA83_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ8586596.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6323..6796)
                     /locus_tag="IYA83_001149"
     CDS             complement(6323..6796)
                     /locus_tag="IYA83_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ8586597.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6865..7020)
                     /locus_tag="IYA83_001150"
     CDS             complement(6865..7020)
                     /locus_tag="IYA83_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ8586598.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7154..7534)
                     /locus_tag="IYA83_001151"
     CDS             complement(7154..7534)
                     /locus_tag="IYA83_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ8586599.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7538..7753)
                     /locus_tag="IYA83_001152"
     CDS             complement(7538..7753)
                     /locus_tag="IYA83_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586600.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7756..8163)
                     /locus_tag="IYA83_001153"
     CDS             complement(7756..8163)
                     /locus_tag="IYA83_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ8586601.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8429..9904)
                     /locus_tag="IYA83_001154"
     CDS             complement(8429..9904)
                     /locus_tag="IYA83_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ8586602.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9894..10742)
                     /locus_tag="IYA83_001155"
     CDS             complement(9894..10742)
                     /locus_tag="IYA83_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ8586603.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10779..11141)
                     /locus_tag="IYA83_001156"
     CDS             complement(10779..11141)
                     /locus_tag="IYA83_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586604.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11441..12065)
                     /locus_tag="IYA83_001157"
                     /pseudo
     CDS             complement(11441..12065)
                     /locus_tag="IYA83_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12062..12373)
                     /locus_tag="IYA83_001158"
     CDS             complement(12062..12373)
                     /locus_tag="IYA83_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586605.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12417..12710)
                     /locus_tag="IYA83_001159"
     CDS             complement(12417..12710)
                     /locus_tag="IYA83_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586606.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12904..13551
                     /locus_tag="IYA83_001160"
     CDS             12904..13551
                     /locus_tag="IYA83_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586607.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13612..14757
                     /locus_tag="IYA83_001161"
     CDS             13612..14757
                     /locus_tag="IYA83_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8586608.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14838..15143)
                     /locus_tag="IYA83_001162"
     CDS             complement(14838..15143)
                     /locus_tag="IYA83_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ8586609.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15168..15599)
                     /gene="ruvX"
                     /locus_tag="IYA83_001163"
     CDS             complement(15168..15599)
                     /gene="ruvX"
                     /locus_tag="IYA83_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ8586610.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15596..15862)
                     /locus_tag="IYA83_001164"
     CDS             complement(15596..15862)
                     /locus_tag="IYA83_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ8586611.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16117..16842)
                     /locus_tag="IYA83_001165"
     CDS             complement(16117..16842)
                     /locus_tag="IYA83_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ8586612.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17203..17273)
                     /locus_tag="IYA83_001166"
     tRNA            complement(17203..17273)
                     /locus_tag="IYA83_001166"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17239..17241),aa:Gly,seq:ccc)
     gene            17390..18142
                     /locus_tag="IYA83_001167"
     CDS             17390..18142
                     /locus_tag="IYA83_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ8586613.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18158..18637
                     /locus_tag="IYA83_001168"
     CDS             18158..18637
                     /locus_tag="IYA83_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ8586614.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18888..20468)
                     /locus_tag="IYA83_001169"
     CDS             complement(18888..20468)
                     /locus_tag="IYA83_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ8586615.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20461..21204)
                     /locus_tag="IYA83_001170"
     CDS             complement(20461..21204)
                     /locus_tag="IYA83_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586616.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21380..22156)
                     /locus_tag="IYA83_001171"
     CDS             complement(21380..22156)
                     /locus_tag="IYA83_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586617.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22404..23240)
                     /locus_tag="IYA83_001172"
     CDS             complement(22404..23240)
                     /locus_tag="IYA83_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ8586618.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23251..24180)
                     /locus_tag="IYA83_001173"
     CDS             complement(23251..24180)
                     /locus_tag="IYA83_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ8586619.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24372..24725
                     /locus_tag="IYA83_001174"
     CDS             24372..24725
                     /locus_tag="IYA83_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ8586620.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24799..25455)
                     /locus_tag="IYA83_001175"
     CDS             complement(24799..25455)
                     /locus_tag="IYA83_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ8586621.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25681..27615
                     /locus_tag="IYA83_001176"
     CDS             25681..27615
                     /locus_tag="IYA83_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ8586622.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27701..28924)
                     /locus_tag="IYA83_001177"
     CDS             complement(27701..28924)
                     /locus_tag="IYA83_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ8586623.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28918..30816)
                     /locus_tag="IYA83_001178"
     CDS             complement(28918..30816)
                     /locus_tag="IYA83_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ8586624.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30810..31928)
                     /locus_tag="IYA83_001179"
     CDS             complement(30810..31928)
                     /locus_tag="IYA83_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ8586625.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32011..32844)
                     /locus_tag="IYA83_001180"
     CDS             complement(32011..32844)
                     /locus_tag="IYA83_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8586626.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32837..33753)
                     /locus_tag="IYA83_001181"
                     /pseudo
     CDS             complement(32837..33753)
                     /locus_tag="IYA83_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(33980..35104)
                     /locus_tag="IYA83_001182"
     CDS             complement(33980..35104)
                     /locus_tag="IYA83_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ8586627.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35287..36195
                     /locus_tag="IYA83_001183"
     CDS             35287..36195
                     /locus_tag="IYA83_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8586628.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36336..37949
                     /locus_tag="IYA83_001184"
     CDS             36336..37949
                     /locus_tag="IYA83_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ8586629.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11653 a         7681 c         6304 g        12862 t
ORIGIN      
        1 tttatatctt attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa
       61 cccatatcaa agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt
      121 tatctatcac tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa
      181 cttcaataag ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag
      241 ctacataatt tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt
      301 cccaagataa cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta
      361 ttactttctt atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat
      421 tttccaaatt tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt
      481 tccaccaatc tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg
      541 ttaaagaatt cataacccta acaccattat ccaaacacat taattcgcta gatttaataa
      601 cagctttaaa atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga
      661 aaataatctc acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg
      721 ttttgaaatt attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta
      781 aatgtagata atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc
      841 ttttgaatta taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa
      901 tcagcttgcc aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat
      961 catctatcat ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac
     1021 aatagatttc gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc
     1081 ttaattatac taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag
     1141 ctatgatcgt tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac
     1201 tcttgttttt ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa
     1261 taataaacgt caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca
     1321 tttgtgaacg cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc
     1381 catcttcatc ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta
     1441 cattcttaat gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt
     1501 actggtatcc gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat
     1561 gacaccgcca aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca
     1621 atagcacttt ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca
     1681 atagtgtgat cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc
     1741 ctaatgaaaa aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt
     1801 tacaactggc gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt
     1861 gcgaacgatc actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac
     1921 ttgatttcta cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt
     1981 tccagcttta ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa
     2041 tgatttacca ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt
     2101 caatgtatct aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg
     2161 gtctaatgcc acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt
     2221 gaatgttttt aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa
     2281 ctgatctttg acctcttgta caatattcac tgctgaatct tccacaacct catttgataa
     2341 ggcaatctta ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc
     2401 aatatcacca atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc
     2461 tactggatat ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt
     2521 ataatttgaa cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc
     2581 tgcatttaca gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata
     2641 cccacgcaat tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt
     2701 ttgtgcttta gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat
     2761 aaacgattcg taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt
     2821 cacgacacta atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc
     2881 ttcttttttg tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac
     2941 cccttttgag atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc
     3001 aaaatgtaat cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag
     3061 tggctttgaa taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa
     3121 tgcttcgggt gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt
     3181 caacgcatag cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac
     3241 tttgggagaa tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc
     3301 attattcaac tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta
     3361 aatacgtaaa gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca
     3421 ttttcgaccg cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt
     3481 ttacttttgt ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt
     3541 caagtttcgt atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga
     3601 tatactggag attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa
     3661 tcggtacacc aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct
     3721 tcaaaacatc ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg
     3781 ttctaagagc attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg
     3841 cgttctttgc tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga
     3901 caccacgagt aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat
     3961 cacgtaacgc ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt
     4021 gcaaaatatc gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac
     4081 cattatctaa ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct
     4141 tttcaccgtt gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca
     4201 aagcaaactt taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca
     4261 ccagttcatt atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa
     4321 tatcactagc aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc
     4381 ttgtaaagct ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat
     4441 ctcccgttgt attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg
     4501 tatttagcat gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga
     4561 catttgttag taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg
     4621 tatgaatgta attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct
     4681 ccttccgttg tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt
     4741 ggatcaattt tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt
     4801 aaaatagcgt tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct
     4861 tcataaactt gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt
     4921 acttctaaca ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac
     4981 gggtcataac aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac
     5041 tgaaatactt ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca
     5101 gctttttcta gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat
     5161 ttggtggcta caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc
     5221 catgaaacgc tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg
     5281 cctgtaatat cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg
     5341 tcctcactcg cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt
     5401 tgtttcaaag caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct
     5461 tcactttcaa aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga
     5521 tcgtccaatt cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca
     5581 cgatctaaca ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta
     5641 ataacacaca ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt
     5701 ttacgagttt ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct
     5761 agcgtgctgg tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc
     5821 ttagagttca taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta
     5881 agtgaattag acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac
     5941 aatacttgcc ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca
     6001 cttgcgatat acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc
     6061 ctgtaattcc cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat
     6121 cctaacatct tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct
     6181 ttggtggctt gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa
     6241 tcgcttatat gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat
     6301 aggactttct caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt
     6361 catcattttt aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg
     6421 aggtggcttg ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg
     6481 ctttctttac tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg
     6541 ctgttttcat tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac
     6601 tcaatggata atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg
     6661 ctgccatcca gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc
     6721 tagcgtccac acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata
     6781 ttttagggtg tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta
     6841 agctcacatt ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat
     6901 gatttttccg ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg
     6961 ctgtttccct gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat
     7021 tatatacttt ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct
     7081 tttcacaacg gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact
     7141 atgccccctt aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg
     7201 aattatggtg tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat
     7261 catcttttag ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta
     7321 ggcaatcctc acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc
     7381 tactttgata gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg
     7441 ccgtttgctt tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac
     7501 atccagcatg attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt
     7561 cgtcagaatc atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca
     7621 agcacgttcc gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt
     7681 ctacatcttc ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac
     7741 tttccatatg cataattatt ctacctccaa taaattagtt atcaattcat catctgttag
     7801 tagcaaagcc aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag
     7861 ctctcgttgt ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc
     7921 acctgtatca tcatagttgc cactatctac caatgatccc actactagcg attgacacca
     7981 cgctactgac tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc
     8041 aaatgtggaa taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa
     8101 ccgatccatt tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag
     8161 catttacttt cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta
     8221 ccactcaatc ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat
     8281 ccagtaacat tattccccag catattagct aactatttca aaagttttaa ggaaataagg
     8341 tgttaccccg ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg
     8401 ctaggtgtta ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat
     8461 tttcataggc atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct
     8521 tccgttccca accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg
     8581 atcgaccatt caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg
     8641 tgagtacgct atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct
     8701 gtaaattttt ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc
     8761 acgctggagc ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt
     8821 ctttaattgg attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt
     8881 ctttatcttc atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt
     8941 cagctaacac ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat
     9001 tcttacaagg caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat
     9061 cattcgacgt tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag
     9121 gaggtctaat tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt
     9181 tgttcattga agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat
     9241 catctttctt ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta
     9301 atgctggaac ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa
     9361 actttattcc tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt
     9421 ctcgtgtgta aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag
     9481 gctgttgatc ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc
     9541 tagccacatt tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt
     9601 cttcaatata tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat
     9661 tgatccaagg agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg
     9721 ttccagctaa attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt
     9781 taatctgatc gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat
     9841 tgtcttcacg ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag
     9901 ccatgagctt tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct
     9961 ttatctttta gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt
    10021 tgattatcta caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta
    10081 ctaccttcgc cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct
    10141 acgggtcggt attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa
    10201 tcgggagctg gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca
    10261 acacttcttg aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc
    10321 ccgcttaaac ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct
    10381 tgttggataa ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg
    10441 atttccgtat aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca
    10501 acgccgtttc gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca
    10561 agcattaacc caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa
    10621 tcgttagttg catcattcca accactagag ccttttattg gaattttagt tccaggagat
    10681 aagggataaa ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc
    10741 aataaacctc tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc
    10801 ttcaactgct caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga
    10861 ttctgatact ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg
    10921 tttaaccatg cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg
    10981 tgttctgcat gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca
    11041 aagtaggttt tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat
    11101 tcaaggctat cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt
    11161 cttaattgat agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag
    11221 atagctattt ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct
    11281 ataattaagt ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac
    11341 aaagaatatc gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat
    11401 ggataaggct ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag
    11461 cagaacgatt ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag
    11521 tcattaattc tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca
    11581 tatctttctt ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta
    11641 tcacattcgc tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag
    11701 tcattgcttc tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc
    11761 ctgtggactg gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt
    11821 cttttttacg gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca
    11881 tcttgtgttc tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa
    11941 gagtgaatgg ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact
    12001 gcccaccatt catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt
    12061 ttcattcaac gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat
    12121 acaaaatttc ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat
    12181 cgttcaagcc acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt
    12241 ccatttgttc gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta
    12301 attctgccgc tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt
    12361 gtgtcgattc cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat
    12421 ttttggtatt cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca
    12481 actataatca actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg
    12541 ttcatatact tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct
    12601 aataatgaaa gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata
    12661 attttaaaag ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc
    12721 cctcgttagc atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac
    12781 atttctatac ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta
    12841 acatactttt ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa
    12901 tatatgaatg gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga
    12961 caaagaatta gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa
    13021 ttatccccac cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc
    13081 aataacacca ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta
    13141 aacggatctt atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt
    13201 gaaaagcaat tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct
    13261 aatgctatca ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca
    13321 aacgcaataa aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca
    13381 attgcttatc tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat
    13441 tcagaaaaaa atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta
    13501 aaagagttca cagctcttgt tactgagcta aataaacact attcaaaata ataataaact
    13561 gcctcctcca accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca
    13621 ttcaagcaat atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg
    13681 aacaaaatga ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa
    13741 gcccagcttg ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg
    13801 aacaacgaaa cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc
    13861 cgagaagcca ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat
    13921 ggaaatcttc gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg
    13981 gctgaaacta ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac
    14041 gctatcaatc ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga
    14101 accaccacta agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg
    14161 tttgattacc taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta
    14221 cgctttctct tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac
    14281 attgattttc aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc
    14341 tataaaatca gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga
    14401 acaatttcta tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt
    14461 gtaactgatc cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct
    14521 atttatgcaa gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat
    14581 gtagcaagac acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt
    14641 caaactagat tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca
    14701 aaagaaacga aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg
    14761 gcatacaata tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt
    14821 tctcctagct ttatagtcta ttcttcttct tccgccatga atgtattgaa tacttcttca
    14881 atcatgtccc attctgcgtc tgtttcgatt tgttctaact cgccttctgt tccatcttcg
    14941 ttttcgatgt atgcataagc ttgtaattct acatcttcat cttctgaaac accagcagga
    15001 tataaaagaa catagttacg tccaaattct tcttgtccgt caactgttag taggatttca
    15061 tataatgttt catttccttg gtcatccacc aatgtgatgt gttcgtgtcc ttcatggtcg
    15121 tgggaatggt tatgttctgt cataagatta cctcttttct tttattatta aaataattgc
    15181 ttacctgagc cgtctaaata attttgtaag atcatcacag ctgctaattt atcgatgact
    15241 ttttttcgtt tggctcttga agtatttgcc tgctctacta gcatacgttc tgcctgaaca
    15301 gtggttaatc gttcatcttg ataatcaact ggcaaggaga atagttcttt gatcttttct
    15361 ccgtatgaca tggaagcttc tgcccttggt ccgatggaat tgttcatatt tttaggtaat
    15421 ccaactacaa accggtctac ttcatattgt gctactaatt ccttcaaccg atcaaagcca
    15481 aactcttctt ctgcttcatt gatccgaatg atctctactc cttgagctgt ccagccaaag
    15541 gggtcactga tagcaacacc tactgtctta gaaccgacat ccaagcccat tactctcata
    15601 aattcactcc atgatttgag aggtagtcct tgacaattac ttccatgatc tcatcgcgtt
    15661 cgtgacggcg aatcaaattt cgagcatcac gataacgagg aatgtaggcc gggtctccag
    15721 aaagcaagta gccgacaatc tgattgattg gattgtagcc tttttcttcc aaagcccgat
    15781 aaacaatcgc taatgtttcg ctcacttctt ttttgcgact atcgtcaaaa ccaaaacgta
    15841 cggtttcatc tgtaaaaccc atgattctac acctctttcc cgcaaggtaa ttccagctta
    15901 ccttctacaa ccttatttta ctcgaagaaa gggaaaacta caaacaaata ttccaaactt
    15961 ccgaataaat taagaaacac tatttgtatg tttttttcct tttttctgta gctttatcaa
    16021 aagtactctt ttcataaagt atacgctata tttctttgaa tgtatgtttt tacacaattt
    16081 tttcataata ttcataaaaa attcgaatag ctgctactaa ttttttgttt ttgttgcttg
    16141 tcctgtctca taattgatcg tgtagccatc ttgaacattg tagataaaga cattgaattc
    16201 aatttttttg tcttcaatgc tttctgcttc caattgcact cccctggcta ccagttcatt
    16261 cccctcaaaa tgaggagtga cacggtagcg tacatgatga tttgtttctt tgatatatgc
    16321 cgcaatatca ttttcgtgag caagcatgta tggagcattc aacgaacgtg tccctgtcat
    16381 caagttttta atgttgttat tttgaccagt cagctggaaa ccaattaaat ggctgcgatt
    16441 atataaccat tgcccgtcac ttaatttctt ttgtttccaa cctgtcgggt caatatacaa
    16501 aggctctctc ttttcagtag ggaacaaatc ttttccaagc atagcattgg cttgtcctac
    16561 tctgttcaat ttatcaatat ttgaatagct ttcccatgtt cctttggaaa ggctaagatc
    16621 ctcttttgta aattccggct gattcccatt gatttctatt tcttgatgtt ttccatcata
    16681 ttctggcaca tccgctactt gagaagcttg tggtgtggaa tcaaaaatag ttgtaatctg
    16741 ctgaaagata tctgaatctt tttgtgtggt ggaaacaccg aatgcccctg caatcacagc
    16801 agcgacgatc cccacgatcg ccttggttgt ttttttgctc atttctttac tcctttacta
    16861 acagctccct aaatcttaac atatgttccc ctataaataa atactagaaa gaaatagaaa
    16921 cagcaaaaaa taaaacctgt tttaagatgg cttaaaaata caaaaaagag acggattcct
    16981 cgtctctaat cagtcaccac tctatctgta aggtatttat tattattcaa aaattttctt
    17041 catactcaag acaatacact gatttcacaa ccgcttggta aacggtgcgc aaaagcttgc
    17101 atctgcggta ataagtccat ccaagttaaa aacataagaa gttgtttttg cttggctgtt
    17161 cttattactt gttgcagagc agctttttca caacctattt taagcgggtg acgggaatcg
    17221 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcaatacaa
    17281 aaacagtata cgcctctttt attccttcct caagtttttt tattctttac gcttctggta
    17341 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
    17401 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
    17461 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
    17521 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
    17581 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
    17641 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
    17701 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
    17761 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
    17821 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
    17881 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
    17941 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
    18001 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
    18061 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
    18121 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
    18181 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
    18241 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
    18301 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
    18361 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
    18421 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
    18481 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
    18541 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
    18601 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
    18661 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
    18721 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
    18781 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
    18841 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
    18901 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
    18961 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
    19021 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
    19081 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
    19141 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
    19201 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
    19261 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
    19321 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
    19381 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
    19441 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
    19501 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
    19561 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
    19621 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
    19681 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
    19741 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
    19801 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
    19861 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    19921 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    19981 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    20041 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    20101 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    20161 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    20221 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    20281 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    20341 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    20401 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    20461 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    20521 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    20581 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    20641 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    20701 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    20761 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    20821 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    20881 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    20941 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    21001 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    21061 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    21121 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    21181 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    21241 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    21301 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    21361 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    21421 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    21481 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    21541 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    21601 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    21661 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    21721 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    21781 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    21841 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    21901 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    21961 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    22021 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    22081 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    22141 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    22201 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    22261 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    22321 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    22381 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    22441 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    22501 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    22561 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    22621 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    22681 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    22741 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    22801 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    22861 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    22921 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    22981 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    23041 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    23101 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    23161 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    23221 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    23281 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    23341 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    23401 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    23461 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    23521 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    23581 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    23641 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    23701 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    23761 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    23821 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    23881 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    23941 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    24001 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    24061 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    24121 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    24181 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    24241 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    24301 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    24361 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    24421 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    24481 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    24541 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    24601 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    24661 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    24721 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    24781 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    24841 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    24901 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    24961 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    25021 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    25081 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    25141 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    25201 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    25261 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    25321 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    25381 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    25441 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    25501 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    25561 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    25621 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    25681 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    25741 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    25801 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    25861 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    25921 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    25981 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    26041 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    26101 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    26161 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    26221 caacaccctg acattttgat tctagatgaa ccaacgaacc atttagatat cgagacactt
    26281 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    26341 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    26401 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    26461 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    26521 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    26581 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    26641 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    26701 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    26761 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    26821 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    26881 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    26941 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    27001 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    27061 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    27121 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    27181 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    27241 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    27301 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    27361 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    27421 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    27481 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    27541 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    27601 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    27661 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    27721 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    27781 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    27841 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    27901 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    27961 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    28021 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    28081 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    28141 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    28201 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    28261 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    28321 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    28381 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    28441 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    28501 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    28561 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    28621 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    28681 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    28741 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    28801 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    28861 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    28921 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    28981 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    29041 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    29101 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    29161 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    29221 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    29281 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    29341 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    29401 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    29461 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    29521 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    29581 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    29641 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    29701 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    29761 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    29821 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    29881 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    29941 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    30001 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    30061 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    30121 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    30181 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    30241 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    30301 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    30361 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    30421 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    30481 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    30541 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    30601 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    30661 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    30721 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    30781 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    30841 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    30901 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    30961 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    31021 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    31081 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    31141 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    31201 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    31261 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    31321 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    31381 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    31441 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    31501 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    31561 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    31621 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    31681 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    31741 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    31801 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    31861 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    31921 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    31981 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    32041 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    32101 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    32161 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    32221 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    32281 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    32341 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    32401 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    32461 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    32521 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    32581 actggctgta gctaaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    32641 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    32701 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    32761 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    32821 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    32881 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    32941 tttcaaaact tgttttatat aaattgaccc tacgaccaaa gctgaatttt ctggtccatt
    33001 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    33061 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    33121 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    33181 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    33241 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    33301 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    33361 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    33421 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    33481 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    33541 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    33601 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    33661 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    33721 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    33781 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    33841 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    33901 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    33961 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    34021 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    34081 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    34141 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    34201 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    34261 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    34321 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    34381 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    34441 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    34501 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    34561 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    34621 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    34681 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    34741 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    34801 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    34861 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    34921 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    34981 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    35041 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    35101 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    35161 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    35221 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    35281 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    35341 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    35401 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    35461 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    35521 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    35581 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    35641 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    35701 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    35761 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    35821 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    35881 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    35941 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    36001 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    36061 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    36121 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    36181 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    36241 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    36301 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    36361 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    36421 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    36481 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    36541 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    36601 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    36661 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    36721 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    36781 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    36841 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    36901 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    36961 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    37021 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    37081 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    37141 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    37201 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    37261 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    37321 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    37381 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    37441 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    37501 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    37561 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    37621 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    37681 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    37741 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    37801 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    37861 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    37921 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    37981 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    38041 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    38101 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    38161 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    38221 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    38281 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    38341 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    38401 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    38461 tgtattaaat aatttaattt ttttgttcct atttatttaa
//
LOCUS       DACIFS010000023        38220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.023, whole genome shotgun sequence.
ACCESSION   DACIFS010000023 DACIFS010000000
VERSION     DACIFS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.023"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IYA83_001185"
     CDS             complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="IYA83_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ8586630.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2146..3117
                     /locus_tag="IYA83_001186"
     CDS             2146..3117
                     /locus_tag="IYA83_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ8586631.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3141..5333
                     /locus_tag="IYA83_001187"
     CDS             3141..5333
                     /locus_tag="IYA83_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ8586632.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5350..5826
                     /gene="ybeY"
                     /locus_tag="IYA83_001188"
     CDS             5350..5826
                     /gene="ybeY"
                     /locus_tag="IYA83_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ8586633.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5804..6205
                     /locus_tag="IYA83_001189"
     CDS             5804..6205
                     /locus_tag="IYA83_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ8586634.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6220..7119
                     /locus_tag="IYA83_001190"
     CDS             6220..7119
                     /locus_tag="IYA83_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ8586635.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7262..8074
                     /gene="recO"
                     /locus_tag="IYA83_001191"
     CDS             7262..8074
                     /gene="recO"
                     /locus_tag="IYA83_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ8586636.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8458..9375
                     /gene="glyQ"
                     /locus_tag="IYA83_001192"
     CDS             8458..9375
                     /gene="glyQ"
                     /locus_tag="IYA83_001192"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ8586637.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9377..11452
                     /locus_tag="IYA83_001193"
     CDS             9377..11452
                     /locus_tag="IYA83_001193"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ8586638.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11836..12615
                     /locus_tag="IYA83_001194"
     CDS             11836..12615
                     /locus_tag="IYA83_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586639.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12656..13600
                     /locus_tag="IYA83_001195"
     CDS             12656..13600
                     /locus_tag="IYA83_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8586640.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13616..14395
                     /locus_tag="IYA83_001196"
     CDS             13616..14395
                     /locus_tag="IYA83_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ8586641.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14408..15106
                     /locus_tag="IYA83_001197"
     CDS             14408..15106
                     /locus_tag="IYA83_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ8586642.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15134..15898
                     /locus_tag="IYA83_001198"
     CDS             15134..15898
                     /locus_tag="IYA83_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ8586643.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15911..17728
                     /locus_tag="IYA83_001199"
     CDS             15911..17728
                     /locus_tag="IYA83_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ8586644.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17742..18965
                     /locus_tag="IYA83_001200"
     CDS             17742..18965
                     /locus_tag="IYA83_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAQ8586645.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18979..19713
                     /locus_tag="IYA83_001201"
     CDS             18979..19713
                     /locus_tag="IYA83_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ8586646.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            19730..20341
                     /locus_tag="IYA83_001202"
     CDS             19730..20341
                     /locus_tag="IYA83_001202"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586647.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIAL
                     ETDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
     gene            20344..21489
                     /locus_tag="IYA83_001203"
     CDS             20344..21489
                     /locus_tag="IYA83_001203"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ8586648.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
     gene            21512..22564
                     /locus_tag="IYA83_001204"
     CDS             21512..22564
                     /locus_tag="IYA83_001204"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8586649.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
     gene            23093..23869
                     /locus_tag="IYA83_001205"
     CDS             23093..23869
                     /locus_tag="IYA83_001205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ8586650.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            23880..24341
                     /locus_tag="IYA83_001206"
     CDS             23880..24341
                     /locus_tag="IYA83_001206"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ8586651.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            24400..25857
                     /locus_tag="IYA83_001207"
     CDS             24400..25857
                     /locus_tag="IYA83_001207"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAQ8586652.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            25892..27061
                     /locus_tag="IYA83_001208"
     CDS             25892..27061
                     /locus_tag="IYA83_001208"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8586653.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            27099..28190
                     /locus_tag="IYA83_001209"
     CDS             27099..28190
                     /locus_tag="IYA83_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ8586654.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(28258..29736)
                     /locus_tag="IYA83_001210"
     CDS             complement(28258..29736)
                     /locus_tag="IYA83_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ8586655.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(30002..30750)
                     /locus_tag="IYA83_001211"
                     /pseudo
     CDS             complement(30002..30750)
                     /locus_tag="IYA83_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            30893..31843
                     /gene="pfkB"
                     /locus_tag="IYA83_001212"
     CDS             30893..31843
                     /gene="pfkB"
                     /locus_tag="IYA83_001212"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ8586656.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31825..33288
                     /locus_tag="IYA83_001213"
     CDS             31825..33288
                     /locus_tag="IYA83_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8586657.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            33285..33743
                     /locus_tag="IYA83_001214"
     CDS             33285..33743
                     /locus_tag="IYA83_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ8586658.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            33740..34714
                     /gene="lacD"
                     /locus_tag="IYA83_001215"
     CDS             33740..34714
                     /gene="lacD"
                     /locus_tag="IYA83_001215"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ8586659.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(34893..35843)
                     /gene="yidC"
                     /locus_tag="IYA83_001216"
     CDS             complement(34893..35843)
                     /gene="yidC"
                     /locus_tag="IYA83_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ8586660.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYHENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(36281..36556)
                     /locus_tag="IYA83_001217"
     CDS             complement(36281..36556)
                     /locus_tag="IYA83_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ8586661.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            36664..37428
                     /locus_tag="IYA83_001218"
     CDS             36664..37428
                     /locus_tag="IYA83_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ8586662.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            37551..38054
                     /locus_tag="IYA83_001219"
     CDS             37551..38054
                     /locus_tag="IYA83_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ8586663.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
BASE COUNT        12869 a         6242 c         7738 g        11371 t
ORIGIN      
        1 tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg
       61 ttattcttct ggtaactcac ttgcttcttg tttatatcct gatacgcgat atacataata
      121 aaagagcaag ctgccagcgt aaccaagcaa tgttgggacg acccagccaa gacctaatgc
      181 agcaaaagga acataatgat gataaaatcc tagtaagctt gctacgacag tttgctgact
      241 cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat
      301 cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagtaagat
      361 taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt
      421 atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat
      481 ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat
      541 agcagtagtg aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc
      601 taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt
      661 attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac
      721 gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc
      781 acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt
      841 gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg
      901 gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag
      961 aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag
     1021 gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt
     1081 ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc
     1141 aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc
     1201 gacaggttta gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc
     1261 taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga
     1321 tccagccatt tgacctaagt gtacaggaaa gatcagatta cctgacccga agaacatccc
     1381 gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat
     1441 aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa
     1501 ataccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt
     1561 ccacttggga aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc
     1621 ttcttcattc acggacgctt ttcgataaag ttatccgcta gcctgataat ggaggcagcc
     1681 acaatgatct acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt
     1741 catttctcac ttacatgact cactgggaat aaatcactga atagcctact gttttcctcg
     1801 acgctttgct gtatatttat ttgttgacgt tataatacac ctgttttttt tgaaatgcaa
     1861 gaatatttca aatattttgt tttctttttt tgaaacaaga aaaataccca gagaatactt
     1921 gctaagttag acggaatact tgtcctctat cttatgcata gcttacatga tcagcttttt
     1981 ttattcaaac gagaccttta gagaatggaa gcggctagtt ttttaacgtt tcttggtact
     2041 tatggttaag caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat
     2101 ggtaaaatag gaaagaatta atcgaaaaaa aggggatgtc tatttttgac ggaagagtct
     2161 agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac
     2221 gacaagcata taaaactgat tgaggaaaca acggaaacaa ccatccatac acgtggagaa
     2281 atcattcaga tcattggtga gcaatcccag atttccttgg ctgcctcagt cattcggaca
     2341 ttacaggaat tgatcaaacg aggaatccac gtaagtacgc ccgatgttgt gacggcattg
     2401 aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc
     2461 aaagaccgta atggaaaacc tatacgggta aaaaacagcg gccaaaagaa atatgtcgaa
     2521 tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt
     2581 cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg
     2641 acgcggcctg ctgtggaagc aggtgaaaat ctaggctttc ttcccgggga tctaaaagaa
     2701 aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat
     2761 catacgaacc gattgatgga acgaggcgta atcgaaatcg cacctttagc ttacatgcgg
     2821 gggcgaacat tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct
     2881 cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat
     2941 acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca
     3001 ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac
     3061 cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct
     3121 ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg
     3181 gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat
     3241 ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta
     3301 tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag
     3361 cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc
     3421 ggatcaagca actatttgaa ttgatcgata agacgaatga tgccatcaac aaatcttatg
     3481 acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc
     3541 ggatcgccgc gctgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gtgttttatc
     3601 aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact gagattcata
     3661 ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa
     3721 gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac
     3781 cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat
     3841 tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga
     3901 tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga
     3961 aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca
     4021 tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa
     4081 atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag
     4141 tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt
     4201 atcccgctgc ttttgcacca cttgttttaa gctttttctt gaatcgaaga gcagggatca
     4261 tggctggtat tttccaagca gtatccgcac ttttcatttt ttatgattcg attggcacga
     4321 actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca
     4381 gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg
     4441 tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt
     4501 ggttaatgct cgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc
     4561 tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca
     4621 gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc
     4681 atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt
     4741 tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt
     4801 tcgttgaaaa cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca
     4861 gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa gattataata
     4921 tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt
     4981 tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg
     5041 gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg
     5101 cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta
     5161 tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc
     5221 gaaaagtgga aaaatcatta atcagcggtt taagtagcac cttccattct cggatcaaat
     5281 atcctaaaat gaaaacagaa gttgagaaga taaaggaagt acaagaagaa tagaaagaag
     5341 cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg
     5401 aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca
     5461 gaaatgtctg ttactttcat ggacaatgcg gcaattcaag taatcaatcg tgattaccgt
     5521 ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa
     5581 atcccagtga tttttgaaga tggagagaat ccattacctc gtgtactagg agacatcatg
     5641 atctctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa
     5701 cttggctttt tagcggtaca tggatttttg catatcaacg ggtatgacca tatgacacca
     5761 gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa
     5821 agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg
     5881 agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct
     5941 tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt
     6001 agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga
     6061 catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg
     6121 agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa
     6181 aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg
     6241 tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg
     6301 ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga
     6361 gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca
     6421 aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat
     6481 gcagtcttat ttatgatcag tgctgatcaa aaacgtggcc ggggagacga ttttattatt
     6541 gaacgtctaa aaaacgtgca ttctcctgtt tatctgatta tcaataaaat cgataagatc
     6601 catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa
     6661 gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc
     6721 agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa
     6781 tacttcatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg
     6841 cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat
     6901 atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc
     6961 ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga
     7021 gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc
     7081 tatctgcaag acttcggcta ccgaaaagac gagtactgat attctggact tcttataggt
     7141 taggtataga aataaactgg ataaaaatcc gtacgttgag aaaaacagcg gaagatcgaa
     7201 acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa
     7261 tatgagccag cttgtagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa
     7321 agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg
     7381 aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta
     7441 tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc
     7501 gtttcggaac atccaagaag atatcttttt gagcggttat ggtacgtatt tgctaaatct
     7561 agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca
     7621 agctctgcag cggatggacc aaggagatga tgcagaaatc atcacgaata tctttgaagt
     7681 gcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga
     7741 aacaaaagga aaatttgact attcttccaa atatggcggt gtgatatgcg agaaacactg
     7801 gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt
     7861 ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc
     7921 aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa
     7981 aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat
     8041 gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa
     8101 actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc
     8161 actgagagga tctattatac aaaccagaaa actgcaaaat gaatacgtac attgaccaag
     8221 atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt
     8281 tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg
     8341 gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt
     8401 tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg
     8461 agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac
     8521 ggctgcatgc tgatgcaagc ttatgataca gaaaaaggag caggtacgat gagcccatat
     8581 acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc
     8641 cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt
     8701 caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt
     8761 cttttaggaa ttgatccatt agaacacgat attcgctttg ttgaagacaa ctgggagaac
     8821 ccatcgatgg gttgtgccgg tgtgggctgg gaagtatggc ttgatgggat ggagatcaca
     8881 caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc
     8941 acttatggac tagaacgtct tgcttcttat atccaagaag ttgaaagtgt ttatgatctg
     9001 gaatgggctg atggcgtgaa atatggtgag atcttcaaac aaccagaata tgaacattct
     9061 aaatattctt ttgaagtaag cgaccaagac ctgctactaa gcaattttga tcgttttgaa
     9121 aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg
     9181 aaatgtagcc atacattcaa tttattagat gctcgcggtg cagtatccgt aacagagcgt
     9241 gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca
     9301 gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacgtg
     9361 aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc
     9421 tgctcatgta gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga
     9481 tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat
     9541 caaagtaaca aacattccag aacgtcaagc agacagcgaa gaagaagtca aaggaccagc
     9601 caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg
     9661 cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt
     9721 ctatgtaacg aaatacactc acggcaaaga agcaaaagaa gtattgacag gtttagcaga
     9781 tgtcattaaa agcctcactt tcccagtaac gatgcactgg gcagaatacg attttgaata
     9841 tattcgccct attcactgga tcgtggcatt gttagatgac gaaataatcc cgtttgagat
     9901 cttggatgtc aaaacagaca gagtttcaag aggtcatcgt ttcttaggcg aagatgtgac
     9961 atttacgcat gcaaaagaat acgaagaaaa attgaatgaa caatttgtta ttgcttcacc
    10021 gcaaaaaaga aaagaaatga tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg
    10081 gatcattgat ctagatgaag atttactaga agaagtcaac aatctagtcg aatatccaac
    10141 tgtcttttct ggcgacttcg aagaaaaata tctatctgtt cctgaagaag tactagtcac
    10201 ttctatgaaa gaacatcaac gttatttcga agtacggaac gatcaaggta tgttgcttcc
    10261 tcattttatt tctgctcgta atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa
    10321 tcaaaaagta ttgactgctc gtttagaaga tgcggaattc ttttataatg aagacaagaa
    10381 agcttcaatc gaccaatacg ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg
    10441 tagcatctat gagaaaatgc aacgggtaca agtcatcgct cgcatcatag gcgaacaagt
    10501 aggactatct gaggaagaat tgatggatct gcaacgcgct gctgaaatct ataaatttga
    10561 tctagtaacc aacatggtag gagaattccc tgaactacaa gggatcatgg gagaaaaata
    10621 tgctctgctt aaaggcgaga aaccagccgt agcaacagcg atccgtgaac attacatgcc
    10681 tatatccagt gaaggagatt tacctgagac tacagtaggt tctgttttag cgattgctga
    10741 taaattagat agtatctttt ctttctttgc agttgggatg atcccaagtg gctcaaacga
    10801 tccatatgct ttgcgccgtc aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg
    10861 ggcctttcct ttgtctgttc tgcaagaaac gattagtgaa gttatcagta aagatacgga
    10921 tcgttttggc attggcttgt cagcgggaca acaacaggtg atcgacttta tcaaaggccg
    10981 tctgcgccaa ttattgacaa cgaagaatat ccgtcatgat gtcatcgaag ctgttttgaa
    11041 tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca aacaacatct
    11101 tgcagatgaa gacttcaaac cttcaatgga agcattgaca cgtgtcgtga acttagcgaa
    11161 aaaagcgcag cttgaaaaac aatcagaagt agaccctgag ctgttcgaaa acgacgcaga
    11221 gaaagaatta cacaaagcag tagaagcaat gaaagcaagt tttgctgacc aaacgatcca
    11281 cgaaaactac gaccagcttg tcgctcttcg tccactgatc gaaaattatt ttgatcaaac
    11341 gatggtcatg gctgatgaac cagcagtacg taagaaccga ctaacacaac ttagtcaaat
    11401 aactgccatg gctttgtcat tagcaagctt ggatcaaatc atcacaaaat agtcttttgt
    11461 ttgaaaaaca gagagcatgc acgaaacgtg ctgtgtctct gtttttttgt tataaatata
    11521 aaagctgtta ttaattctat tttaataatt tttgttattt tattctttgc tagaattttc
    11581 aaaacgctca tgaaaagtga ggaaataaaa atgaaaaaga ttaataataa tcttgttact
    11641 gttttattta cttttaaatt aggaatttcc ctagtcaaga aaactgtttg tggtatgatt
    11701 atatagggaa aacctgttta taaatatttc tatgaatata ttagtttaat tatgctttta
    11761 aaataaaaag cgctcattct gatgtgaatg aatcacatct ttatttatct atcatcaata
    11821 tttaggagtg aatctatgga aaagccgttc gtcaagttat tggctactgt ggcggtaggc
    11881 acaggcgcta ttgtgatttg cttgtttgga taccatctca ataatcagcg gcaacatcat
    11941 caacggatca attacgcaga atctgccatc accaatcaaa aagacaccgt gacctcttta
    12001 tcaaaagaag tagataagct gtacagtaca aaagaaaaaa ttttcctaaa tcctgaaata
    12061 acagaagaaa cagtttcaaa tttgtcacat aaactatctt ctatcaaact ttcagcagat
    12121 gattatgaca tcaaggaatc agaattacct aaagaagctg ctgcgatcca agaagaaaag
    12181 aaagcagtct taacacagtt agaggatgca gaatcaaaac tgaagatcca aacggctgtc
    12241 aataaattat ttactaaaaa cgtatcaaac tggcagcaag ctgtggatga tgtcatcatc
    12301 aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg aaaatatgag cttttttaag
    12361 gatagtgctt ggaaaacggt cgttatgcaa tatctaggat tcgctgatac ccaaattgca
    12421 caagtcacgc aactagatca gctattcgat acgatgctga aagacggtca agtaacagct
    12481 actgctactt acgatcaata tttgaccgca ctcagtctca tcgaacagat ccggaatgaa
    12541 aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg ctcagcaaat gggctatagc
    12601 aatacgagct attaataagc gagaattcaa gcaaggctag caaacggagg aaactatggc
    12661 aaaacgaaga agaagatcaa gagggaaaaa gatatttcta attattttag caattcttgt
    12721 cgtattagta ttagcaatcg taggagtagg agcaaaatta tatctagatc tctcacattc
    12781 tattgaaaaa acttacgaat cagtggaacg aacccaagga gaacgaacaa aaccagttga
    12841 tctggacaaa caagaatctt tctctgttct attgatgggg attgatacgg gagatttagg
    12901 tcgggtcgat caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa
    12961 acagacaacg attgtcagca ttcctcgtga tacgtatgtc gacatcgttg gacatgacac
    13021 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
    13081 acaaaaatat gtagacatcc caatcgatca ctatgtttca atcaatatga aaggtctaaa
    13141 agaactcgtt gatgcagttg gtggtatcga agtcaataat gacctcacct tctcacaaga
    13201 tggctatgat tttacgatcg gtaaaatcac attgaacggg gatcaggcat tgtcttattc
    13261 tcgtatgcgt tatgaagatc caaatggtga ctacggcaga caagaacgtc aaagaaagat
    13321 cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt gtaagcaact atcaatccgt
    13381 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaagat
    13441 tgctttagat tatcgggatg catttggtac gatcaagcaa gatcagctgc aaggagaagg
    13501 cttcatgcaa gacaatatct cataccaaaa agtcagcgat caagaacttg atcgtgtgaa
    13561 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
    13621 agagacaatc agcttgaaag aaatttttga aacattacgc aaacacgtga taacaatcct
    13681 catctcaatg tttgctggct tggccatctc aggcatcgtg acgtttttca tcttgacgcc
    13741 aaaatacagc tcacaagccc aattgatcgt gacactgccg caatcagata ctgcaaatgt
    13801 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaggatatga tcgtcagtga
    13861 tctagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtaa aaatgactgc
    13921 tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
    13981 ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc gctaatacaa cgtcacaagt
    14041 attccaagag aacgccaaag acgtgatgaa tgtcgataaa atctcgatca tctcaggtgc
    14101 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
    14161 gttaggaatg atggtaggcg taggtctagc tttcttattt gaattactcg accggacagt
    14221 aaaagatgaa aaatatgtga cggatacatt gggcttcccg atcttaggga ctgtaccaga
    14281 aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa ggaccagctc cgacacaacc
    14341 aaaagctaca aacgataaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga
    14401 aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
    14461 cacactcaca aacccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
    14521 ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt gttgtgactt catcaggacc
    14581 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
    14641 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
    14701 gaacaacgca agcggattaa gtaccgttct tagttcatca ggaagtgtag cagatgagat
    14761 ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt ggacctaaac caccaaatcc
    14821 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
    14881 tgatgtagtg atcttcgaca tgccgccagt agttgccgta acagatgcac agatcatgtc
    14941 ttccaaaacg gatggcacct tattggtcgt acgtgaaaat atgtcacgga aagaatcatt
    15001 gactaaagca aaagaattgc tggatatggt acaagcgcga gtccttggtg ttgtatacaa
    15061 tggggcagaa cacagcaaag atgctggcta ttactacggg aattaagaaa taaaaagaga
    15121 ggagacaaga gaaatgatcg atctacattg ccatatcctg ccaggaatcg atgacggagc
    15181 agaaaacata gatgccagta tcgccatggc tgaaaaagcg attcaacaag ggatcacaca
    15241 tattttatgc acaccccatc ataataatgg aaaatacagc aatgaaaaat cacaagtcat
    15301 ttctctagtc gcttcgttac aagcagaatt agagaaaaga caattgcctt tgacgttatt
    15361 ggaaggacaa gaagtacgta ttaccggaac actgatcgaa gacatcgacc gagatgaaat
    15421 tttatttaca gatttagatg atacatatct tctaatcgaa tttccgacac tggaagtgcc
    15481 gttatatgca gaacagctct tttttgagtt gttacaacta ggaaagacac cagtgatcgt
    15541 ccatccagaa cgcaacgcgc attttcgtaa agacccgaat catatgctgc cattcctaga
    15601 tatgggatgc ttggcccaac tcaccgcacc aagttatgtc ggtgtatttg gaaaagacat
    15661 ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg cagatggctg cttctgatgc
    15721 ccatggggtc tctaagcgca ccttttactt aaaagagtgc tataagcaga ttgccaagga
    15781 ctttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
    15841 tgaaatcagt tatccagtct atgaagagat aaaaaagaag aagtttggtt tattttaggg
    15901 ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt gccacattgg ttgttgtgga
    15961 cagtttgtta ttgatcgctg caaatattgc agcagctaaa ttcatgaagc cgtttgtagc
    16021 tataccaatg gaccttattt taatctctac cggtttgtcg attggttttt atttatttta
    16081 tggtagttta ttcaaagttt tcactcggat caatcgctat acaaatttaa gagagatcat
    16141 tgcaattttt ggttcactat ctgcaagtga cgtctcaagt atcctcatac tgctgttcat
    16201 taatcggcaa tacagtttac ggttagttat ctttgcttat ctgctttcgc tcttattgat
    16261 catcggtagc cgattgatct ggcgtatcta catcgaaaca aaaaatatga gatatgtatc
    16321 agcggattct gctaaaaata cattgatcgt tggtgctggc gaaggcgggc gtattttata
    16381 caacagcttt ctgggatcca aaacggctca agatatccaa gttgtcggat ttgtagatga
    16441 tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga
    16501 tattccggaa ttgattgaaa aatatgacat tcaaatggta acgatcgcta tcccttcctt
    16561 atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa tccgctcacg tgaaagtcaa
    16621 cacgatgcct tctatagaag aacttgcttc aggaaaaatt agcgtatcaa aattaaaaac
    16681 aattgacgta gtggatttac tgggaagaga tgaggtaaag ctggatattg aatccatcaa
    16741 agatcagatc acagacaaag tcattcttgt caccggcgca ggaggatcga tcggttcaga
    16801 aatctgtcgg caaatcattc aattcaatcc tgcaaagcta ttgcttttag gacatgggga
    16861 gaactcgatc tacttgattg accgtgagtt acgcacgcat catcaaaatt gtcctacaga
    16921 aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa atcaatgaga tcatggaaca
    16981 atatcatcca gatatcgtct accatgcagc tgctcataaa catgtaccac tgatggaata
    17041 taatccaaaa gaagcagtca aaaacaatat tttcggtaca aaaaatgtag cagaagcagc
    17101 taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca gataaagcta acaatcctcc
    17161 aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc gtgactggat tgaatgaaga
    17221 aggctgtacg aaattctcag ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt
    17281 gattccagta ttcagagaac aaattgctaa aggtggtcca atcactgtaa cagactttcg
    17341 tatgacccga tacttcatga cgattcctga agctagtcgt ttagttattc aatctggcgc
    17401 attagcaaaa ggtggcgaaa tttttattct agatatgagt gaaccagtga agattgtaga
    17461 cctagccaaa aacatgatcc gattgagcgg gtatagcgaa gacgaaatcg aaatcattga
    17521 aacaggtatc cgtccagggg aaaaactata tgaagaacta ctgttggata aggaaagaaa
    17581 tgatgaagca gtttatgaga aaatctttgt aggaaacatc aaaggttact caatacaaaa
    17641 agtgatggat tttgtcaaga gcctgccgca agacgatgaa caattggcta aagatatcgt
    17701 cacttttgca aatgcttcga ataaataggg gggaaatata aatgaataaa ttagaaaaac
    17761 gtcagatacc attttcaccg ccagatatta ctgatttaga aataaaaaac gttgtagaag
    17821 tattaaaatc tggatggata actacgggac caaagacaaa aaaattagaa aaaaaaatta
    17881 caaagtggtt aggaacttct aaagtagttt gtttaaattc acaaactgct gctgcagaaa
    17941 cagggttacg tttgctggga ataggagaaa atgatgaggt tatcgtccca gcttatactt
    18001 attctgccag tgcatcggtt gtatgccaca taggcgcgaa cctagtatta atagactgtc
    18061 aacaagattc tttggagatg gattacgatg cattagaagc agcaattacg gagaagacaa
    18121 aagtgattat tcctgttgat cttggtggta taccttgtga ctacgaccgt atttttgaga
    18181 ttgtggagaa aaaaagaaat ttatttcaac ctagtaatga gattcagaaa gcttatggtc
    18241 gtataattgt tatggcagat tctgctcatg catttggagc taattggcat gatcagatgg
    18301 ttggtaatgt tgctgacttg acaacatttt catttcatgc tgttaagaat tttacaactg
    18361 cagaaggtgg agctttagta tggaaagagc acgacaaact agataatgag gcaatgtatc
    18421 atcagtgcca gctgctttct ttgcatggac agtcaaaaga tgcacttact aaaacacagt
    18481 tgggtgcatg ggaatatgat attgtgggtc catggtataa atgtaatatg actgatgtta
    18541 ctgcatcaat tgggttggca caatttgaac gctatcctaa tattctgaaa agaagaaaga
    18601 atataattaa gaggtatgat gaagcctttc aacaactagg aattgaagtt cttatgcatt
    18661 atacggatga ttatagttct tcaggccatt tatatattac tagagtacct aacattagtt
    18721 ctgagcaaag aaatgaaata attataaaaa tggcagaaca agggattgct tgcaacgttc
    18781 actataaacc acttccaatg tttacagcat ataaaaatat ggggtttgat attaataact
    18841 atcctaatgc ctatgctcat tttgtaaatg agattacatt accattgcac acgaaactta
    18901 cagatgaaga agtagaatac gtaattaata actatagtaa gattgtaaag gatttggttg
    18961 gatgatagga gaggcaaaat gagaaagtgg gaagatttgc caaaagaaat gcagaatgat
    19021 gaaatacggt attattatga tatcctcagt aaaaaaaaat tcagcttagt ggtaaaaaga
    19081 gtatttgata ttttagtgtc tgcaactttg ttactgacac tttcatggtt attttttatt
    19141 cttgcaatag tgataaaaat tgattctcct ggacctgtat tttatagaca agaacgtatt
    19201 actcgttatg gaaaaaaatt taaaatttat aagtttcgta ctatggtaat aaatgcagat
    19261 aaagatggca ctcttgttac tgtgaaaaat gatgatcgta ttacaagagt aggaaaggta
    19321 atccgtaaat atagattaga tgaaattagt cagctaatta acgttttggt cggagatatg
    19381 tcttttgtag gaaccagacc agaagttgga aaatatgtaa agtcgtattc accattaatg
    19441 ttagccacat tattattacc agccgggatt acatcagaaa ctagtatttt atacaaagat
    19501 gaagataaat tgttgcatga taaaaataac atcgacaaaa cttatataga aaaaattctt
    19561 cccagtaaaa tgtattacaa tttgaaaagt atcagtgaat ttagttgtat gaatgatttg
    19621 aaaataatgt gtaaaactat ttttgttata tttggtaaag aatatcacgc cgacttagaa
    19681 ggtgaacgag agtttattaa tcagataaaa tgaatggagg ataagagttt tggtgattga
    19741 agagataaaa agttcggata aagagttaat taattcgatt gtcgatattc atatgagaac
    19801 atttacaaat ttttttttga ctttcatggg aaaaaagttt ttggaattga tgtatcgttc
    19861 ttacacagaa tataatgatt ctggtatatt ggttgctatt caaaatgata agccaatagg
    19921 atttcttgca tattctggag atatgagtgg tttatataag tatatgataa agaaaagaat
    19981 aataccgttt ggattttata gttgtgtagc ctttataaag aaacccaata tccttatgag
    20041 gctttttaga gcttttctaa aaccaagcga aacaaaacgt gatgaaaatt atgtggaatt
    20101 agcatccatt ggagttaatc cgaaagagaa atcgatagga gtaggttcca tgcttataga
    20161 taacttaaag agtagaattg atttttcaaa atatgaatat attgcactag aaacagatgc
    20221 attaaataat gatgttgtga ataattttta taaaaagaat gagttcattt tagaacgtaa
    20281 atattctact aatgaaggaa gaattatgaa tgaatatcgg ttttatatgg agaagaatta
    20341 attatgaaat tattgtatat tttaaatgta gctaataaag taaataactt ttctaaagct
    20401 agtatgatgg cagctaaaca aacgggttta gaattttata tagctggtaa ctggaaatac
    20461 aaatccgatg aggaaagaaa agctgatgaa gtgaaatatg gaataaaaat ataccaagtt
    20521 gatttttata gaaacccact aaatttaaaa aatataaaag cttatcatca gattgtagag
    20581 attataaaaa atgaaaaaat agatattatc cattgtaata cacctattgg aggtgttgtc
    20641 ggacggattg ctggtcttaa atgtaaagtc aaaaaagtaa tttaccaggc acatggattt
    20701 catttttatt caggtgctcc taaaataaat tggtttatat attatcctat ggaacgttgg
    20761 ctagctcatt atacagatgt tttgattaca ataaatcatg aggattatac tttagctcaa
    20821 aaacttcgtc ttaggaaagg tggaaaagct tattatattc caggtgtggg tatagataca
    20881 aataggttta tgtcttccac atcaataagg aattttagag aaagtatatg taaagaactt
    20941 aatatacctg atacagcaaa aatcatttta tctgtaggag agctaaatga gaataaaaac
    21001 catttatcag taatacatgc attacaaaaa attccgctgc atacagatat ctattatatt
    21061 atcgctgggg taggagaaat gagggagaca ctacaaaata ttgctaatga attaggatta
    21121 catcagcgag tttttttact aggatataga agtgatatac ccaaattatt agctgctgca
    21181 aacatatata ttctaccctc attaagggaa ggcctcaacg cctctttaat ggaagcgatg
    21241 gctagtggtt taccatgtat tgtcggtaat ataagaggaa atgtagatct tatagaagat
    21301 ggtaagggtg gatttctaat caatcctaaa gacgaagaag aaattgctgg tgcgataaat
    21361 agattggctg ataacaaatt attgtgtgaa tctatggggg cacataattt agacaagata
    21421 aaagattttg atttatcagt tgtgataaac caaataaata atgtttacca tgaaattgta
    21481 agtaaataat tgaattgaaa gtgatattaa tatgaaaaaa ataaaaatta tgcatttgtt
    21541 gcaaagtaat agattttctg gcgctgaaaa tattgtttgt caaataatga gtttatttca
    21601 aaataacgaa aaatttgaga tgatttacgt tagcccagat ggtcctatag caaatatttt
    21661 aaatattaga ggtttaagtt acttaccatt aaaaaaattt tcgagaaaag aaattgacag
    21721 agccgtacat gaattaaacc cagatataat tcatgctcat gattttaatg ctagtgtaag
    21781 agcaagtaga tataagaagc aaatcatcct ttcccatttg cacaataatc ccttgtggtt
    21841 acctaaacta gacataagaa ctattatata ttctttatgt tcgtctaggt ttaattcagt
    21901 aattggtgtt tcgaattccg ttaaggaaga atttcgttac aaacaattaa taaaagataa
    21961 atttgtgttg ttacctaaca ctgtaaatac tgaaagaatt ataaaaatgg ggaatgaatt
    22021 tgttgaagat gatattgata ttttatatgt aggacgcatg gcagaaccta aaaatccaat
    22081 aggtgcaatt aatgttttag aaaatgtagt aaaaaaatat tcaaaacatt tacgtgtcgt
    22141 gatgattggt gaaggaccat tatttgatga agttaagatg caaatacaaa aaagaaactt
    22201 aacagatgtt attgagttaa agggattcaa aagtaatccc tataagtaca tgaagaaatc
    22261 taaagttcta ttaatgccat ctctttggga agggtttggc ctagtatcag tagaggcaat
    22321 gttgttagag acgcctgttg tagcttataa cgtaggagga ttgaaggata ttatcgatga
    22381 gacaaatgga tttctgtgtg gtagtgaagt tgaattatct aattcactaa tgaagctatt
    22441 aaattcagaa gaacttagaa aaaaaatggg agaaaatgct gctattagtg caaagaaatt
    22501 ttctaatatg aatcgttatc aaggagtatt agaaaaaatt tatgtatctg cagctttaaa
    22561 ataaaatatg aattataaaa atagagatca attagtttta cgaaaaataa tacccattag
    22621 tattttgttt caatcaggaa tgatttttta ttcactgatg ccctcaatag caattgcagt
    22681 tgtttctttt tttatgtttt tgtattatat gtggtgctta aaaaagtatt tttgtttagc
    22741 agatagctta ttgttaacat caattctttt cattccaaca tcaactatat ctatattagg
    22801 aactagttat tctagcttac cattgacgtg gcataatcta ataataataa ttttgatgtt
    22861 atatattatt atagaaggaa cattaaatcg ttcatacttg attattttat ttttatttat
    22921 ttgttttgga atatcgacgt ctatgttctt acctttttta atagatgcat taaagcaatt
    22981 aatgactatt tcgttgttta tctgtagttt tttaatagga aaacgtttta aaatgtatag
    23041 caaaaactta actatgattg ggtggaattt ctatttcata ggagtagtat ctatggcgtt
    23101 tcaggtatat ttgcaacgag tatatatttt atctactggt gtaataatag gccattatgc
    23161 agaaatgggt ttaaatagaa ttgcatatgc aggtttgatg ggagatttta gttttgcagg
    23221 gttatatctt gctacaggct gtctatatgc ttttctaaaa tatgtggatt taaaaaattg
    23281 gaaattatta ttctttataa taatagaagt atttttatta ggtgcaattt tatgtgttag
    23341 tgcaagaaca gggttagttt ccttgtttgt cacaatgaca ttgtattatc tatttaatat
    23401 aaatagaatt agtgttcctc atcttttagt tctaataggt ggaatcataa ctacacctta
    23461 tatactagta atgttaatgg caggtcgttc tggacaaagt cttttggatt cttcaggcag
    23521 attagaaaac tatatgtcct ctttaaatgc ttttagtgat aaatacttaa taggatatgg
    23581 attaggatta aataatttat acacgctact aggtgtagca gtaccacata atttttttat
    23641 tcaatattta ttacagacag gaattattgg tacgctaatt attttaagtg gcttctttaa
    23701 atttacgtat gaagaattaa agaaatcagg ttgctataaa tggttatttt tgatgatagt
    23761 agtgggagca atgtttatac cggatattgt atcttcgcgt tttctttacg caatagttgt
    23821 tttgtgtatg atttctgcat cagaaagaac tataaaaaaa gaaagttgag gtagaaaaaa
    23881 tgagaagaaa agcagagaaa tgggcagata aagcttgtag acaatacagg aatggtcaaa
    23941 ataaaatgtc aaaaatatcc tgctatattt taaggattat ttatggatgt gaattctacg
    24001 gacaggtaga ttttagctct aatgctcaac tagtccataa tggtcttggt tgtgtattcc
    24061 accctaaaac aaaaatcggt aataattgta aaatatatca aaatgtgact ttgggtggaa
    24121 atggcaaaat aataaatgga aaagaaactg ttggggcacc aacgctagag gaaaatgtgg
    24181 ctgttttcgc tggtgcatgt gttttaggac caattaatat cggacatgat tcaatcatag
    24241 gtgcaaatgc tgtagttaca aaaagtgttc caccatatag cttagtatat ggaaatccag
    24301 caatcataac agagttgaaa tttgattata aatttgatta aaaagtaatg tttaatatgc
    24361 gaaaggttgc tatatttatt tagcaaaatg gggtaataaa tgggaaataa tattcatgga
    24421 aagacagcaa aagctttaaa atggtcatcc ttagctgaaa taattgcaaa gattataact
    24481 ccaatttcga atatgatttt agctagatta ttgactccag atgcttttgg agtagtagcc
    24541 actgtaacta tggttattag tttcgctgat atgtttagtg attcaggatt tcaaaaattt
    24601 ttagtacagc atgaatttga taatgaagaa cacatgtttt catcaacaaa tgtagctttt
    24661 actagtaact taggtatttc tttattatta tggttattta tttgtgtgtt taataataaa
    24721 atagcgcact tagttggaaa ttctggactt ggattagtaa ttatagtagc tggagtttct
    24781 cttccaatta cttctttttc cagtatacaa tcggcaattt ttagacgaaa ttttgattac
    24841 aaaattttgt gttatgctag aataatatct gcattgatac ctttagttat tacagtaccg
    24901 ttggcgataa ttggactaag ttattggtca ctagtaattg gatctataat aggaaattta
    24961 gttacagctg caatattaac taagttatca gagtggaaac caaaactttt tttttcaaaa
    25021 aaagaatttt acgaaatgtt ttcctttagt gcgtggtcac tagtggaatc attaggtaca
    25081 tggttgtcat cttatatagg tacttttatc gtaggaggca ttttaagcgt atactatttg
    25141 ggaatataca aaactacaat gacaacggtt aatggtattt ttgcaattgt aacttcagca
    25201 acaacaactg tattattttc aacattatct cgtttacaaa atagtaaaac ggagtatgat
    25261 aagacatatt tagattttat taaaattgtt tccattttta ttattcctct gggtataggt
    25321 atatatgtat atagggaact agtaacaaat attctactcg ggagtcaatg gaaagaggct
    25381 gtaccatttg ttgggatata tggattaatg agctgtttta ctcttgtctt gggacaatat
    25441 gcctctgagt actatcgtgg tttaggaaaa ccaaaagcaa atgttcttat gactatccta
    25501 catttagcag tattaattcc agttctaata atctattcaa ggaaagggtt tatagaacta
    25561 gcatatggta gatctttcgt taagatagag cagattatag tcttttggat tattttatgg
    25621 tttggtttta agtttaatcc atttaaaatt ttaaagcagg tttggaaatc ttgtgctagt
    25681 gcattgctaa tgggagttct agcaaaaatt atgttggaat tctgtactaa ttatgttatg
    25741 caattacttt cagtgtgtct ttgtgtaatt ctatattttt ttatatatag ttgtatattt
    25801 aaaggaaaaa cagaaataat taatatggtt agaatgttta ctggtaaagg atattaagag
    25861 agatttttat ggcaaggttg tgattaggaa aatgaaaaaa gtaacaattt taattcctgg
    25921 aatgtattat ggtggaatgg aaagagtagc ttttataacg cgtcaaatac ttttgaaaaa
    25981 taattataat gtatcatttg taactctttt tgataacgat gcagattatc agccaaattt
    26041 tgaatattta tcattaaaat gtgatataaa gccatcaaaa tttggtaaag taattaacat
    26101 ttttaaaaga gtttataaaa tgaaaagtgt caaaaaaaaa ttgaaaacgg atataactat
    26161 atcttttgga caaagttgta atttagtaaa tgttttatca aagagagatg ataaagtata
    26221 tgttggaatc cggtcctatg actggctatt tcaatatata ttccattact atgtagataa
    26281 atttgtatat aagaaagcag atgctgttgt gtctgtcagt cagttaatta aacgtgatgc
    26341 tgaaaatatt ttcaatttta ataaggataa aagctatttt ttatataatc catatgattt
    26401 agagttgata aataataaga gtaaagaaaa aattacagaa atagaggttc caacagacaa
    26461 aaaaattata gtgacggttg gtcgattaga agatcaaaaa ggtttatatc atctaattaa
    26521 agctatatct atgttaaaga acaaagataa tgttgttgtg tacatcttag gacatgggac
    26581 aaaaaaagaa ttattagaaa atttaataaa agcatatcaa ctacaaaacg taatattttt
    26641 gctaggtggc caatcaaatc cttataaata catgtcacgt gcagatttat atgtcatgcc
    26701 atctataact gaaggttttc ctaatgcatt agttgaagca atgtctacag aaactgctgt
    26761 cttatgtgca gattgcaaat ctgggccaag agaaatatta tcgaatagaa cgctagacac
    26821 gatttgtgaa aatatagaat atgctgaatc aggcatatta gtatgtccaa tgacagacag
    26881 tagaaattat tcgccccaat atgaagaatg tgataaaaat ttagcgaatg ctatagaaga
    26941 attgattgaa aacgataatt tgaggaataa gtatgcaaaa gcagcaaaaa agagagcaga
    27001 actatatagt tatgatgcct ttgaacataa cttgttgcaa attatgaaga catttaatta
    27061 attcatatga tgaaataaaa ataagggagt ataactagat gctgaaaaaa aatacatctt
    27121 tacagaataa aacaatatta ataactggcg cagccggttt tattggatca aatttggttc
    27181 ttgagttatt acgtactcag tcatcaataa aaatcattgg cattgacaat atgaatgatt
    27241 actatgatgt ctctattaag aactatagat tatcagagat agaaacggag gcaaagaacc
    27301 atccaggatc tacttggaat tttatcaaag gttctattgc tgataaagca acaattgatt
    27361 tcctctttga taattataaa ccaagtatcg tggtgaattt agcagctcag gcaggagtcc
    27421 gttattcaat tactaatcca gatatgtata tcgaatctaa cataatagga ttctataata
    27481 ttcttgaagc ttgtcgtcat tcatatgata atggagaatc tggtgtggaa catttagtgt
    27541 atgcctcatc gtcttccgtt tatggtacta ataagaaaat tccttattct acagaggata
    27601 aagtagacaa tcaagtatcg ttgtatgcag caactaagaa aagtaatgaa tcaatggcac
    27661 atgcatattc taaactttac aatattccgt ctacagggct acgtttcttt acagtatatg
    27721 gtcctgctgg tcgtccagat atggcttatt ttggttttac tgataaactt cgtgctggtg
    27781 ataatatcca aatattcaat tatgggaatt gcaagcgtga ctttacctat atagatgata
    27841 ttgttgaagg agtaaagagg gttatgcagt gcgctccaga gaaaattgat ggggaagatg
    27901 ggctaccaat cccaccatat catatttata atattggtaa caatacgccg gaaaacctac
    27961 tagattttgt agatattcta caacaagagc taattgcagc aggggtgcta ccagaagatt
    28021 acgactttga atctcatacc gaactagttc caatgcaacc aggagatgtg ccagttactt
    28081 atgcagatac tagtgcgtta gaacgagatt ttgaatttaa accaagtaca agtttgcgtg
    28141 acgggcttcg taaatttgcg cagtggtata agtcttttta tgaagtataa ttgtattttt
    28201 ttacttataa aagtttatag aaacacctca gacactacaa atctgaggtg ttatttttta
    28261 atctcgttga aataaatctc tggtatatac tttttctttt acatcatcaa gattactatc
    28321 gtaacgatta gcaataatac tgtgactgat atttttaaaa tcatctaaat tatttattac
    28381 ttttgaacca aaaaatgtag tgttattttc aagtgttggt tcataaatta caatagttgc
    28441 cccctttgct ttaattcgct tcataactcc ttgaatagat gactgacgga agttgtctga
    28501 attactcttc attgtaagac ggtatacacc tatgactacc tctttttctt tttctgcatt
    28561 ccacgaactg ttagcctcat acgtgccagc aagttctaac actcgatctg ctataaagtc
    28621 ttttcttgta cgattactat cgacaattgc acttatcata ttctgaggaa catcgttata
    28681 gttagtgaga agttgttttg tatctttcgg taaacaatag cctccatatc caaaagatgg
    28741 attattataa tgtgtgccaa ttcttggatc taagcaaacc ccgtgaatga tttcttgtgt
    28801 atttagtcct ttcatttctg cataagtatc cagctcattg aagtaagata ctcgaagtgc
    28861 aaggtaagta ttagcgaata atttaactgc ttcagcttct gtgaatccca tgaaaagagt
    28921 ttcgatatct tttttgattg ctccttcttg gagtaaatct gcaaaagtgt gagaggcttc
    28981 tcgtaaagtt ttgtcagttt ggtccgtgcc gacaataatt cgcgaaggat atagattgtc
    29041 atataatgcc ttagattctc tgagaaattc tgggctgaat agaatatttt tagtattgta
    29101 ttttttacga atactttcgg tatagcctac gggtaccgtc gacttgatga ccattattgc
    29161 ctttggattg acttctaaca ccaatttgat tactgactct acagcagaag tatcaaaata
    29221 attttgttta ctatcataat ttgttggagc agcaatgaca acaaaatcgg cttctctata
    29281 ggcggctgca ccatcagtcg ttgcaattaa attaagattt ttttcagcta gatatttttc
    29341 aatgtaatcg tcttgtattg gagatttctt attgttaatt tgttttactt tttcaggaag
    29401 aatgtctact gctgttacct gattgtgctg ggcaagaaga gtagcaatgg aaagacctac
    29461 gtaaccagta ccggctacag caatattctt cgactgatgt tttgttctgt ttgatttatt
    29521 tgattcattt ataaatagat ctgttggttc aaaatttaga acttctgcta atgattctaa
    29581 ttgttgtatt gacgggataa aatcgagaga ttctactcga ctaattaagg cacggttgat
    29641 accagtagct actgcaagtt gttgttgtgt cattttcaga gattttctag aatcaataat
    29701 gatttgagct aaacgttttt gagataactt tttcatgatg cactcctttt tttgttgcta
    29761 ataataacaa acaatttttt cttctataac agaatagctt attattaata aaactgtcaa
    29821 tttttttatt gattaaaaag atattttttg attttaagta ttttcaatta ttttttgtta
    29881 cttaaaataa ctttatagat tgtgaataac tttatacata aaaagtttac gtgagtccat
    29941 acaatataaa gaataaaaaa tgtgatgttt taaactgtaa ccaaccataa taaactgctt
    30001 ctcaattctc cctcatacgc aatacatagg cgtcaagcag gattctcgcg atgatctgga
    30061 gaggcaagcg tgaacgtact tcataatcag ggaaataaga accttctgat ttgaaaccta
    30121 cgagatttat ctcgcaataa tctttgagtg tagaggaatg attggctgta actagaatcg
    30181 aaccaatctc tgcatcatag cagttctttg cggcggtgac gagttctttt gtctcgccgt
    30241 tcaaagaaac aaaaataacg ctgtctttct tagttagttt ccgactgatg cttttgataa
    30301 tctctggatc agtatgcatt tgacaatttt ttcctgttaa ttgaagtttt acgagcatct
    30361 cttgaccaat catttctgaa aatcctcggg caaagacaaa aacccggtca gtaaatttga
    30421 ttctttgtat tgcatcttca atgttcccga tttccaacat attgatcgta cgtaccactt
    30481 cttgttcatt ttttagaatg gctcgacgga tacctttgtc tactttatct aaaaagaaaa
    30541 attgatgttc ttgttttctc cttcttttag atggtgctta aacgaagtaa atccttcata
    30601 gccttttttc ttcattgtcc gcacaatggt ggctgttgag acatttgctt gttcacttag
    30661 tttcacgatg gagtagttag ggatctctag gatgtggtgt tcgataaagt tccagaggta
    30721 ctgttctgtt tcacttaatt tgtgatccat attctttcct tctttcacta aaagttagtt
    30781 aaaaaatagc acgatgtgtt tataaatgta aaggttttct ttgtgttgga acgattaaga
    30841 aaacattttc tctttcgcgt atttcataat agattcaagg aggtcattgg ttatgtctat
    30901 ctacacatgc acgatgaatt tggcaattga tctatttatt gaaacagagg agatgcatcc
    30961 ttttatggta aaccgtacaa aggaggatga cattcaggca aacggaaaag gagtaaatgt
    31021 gtcactagta ttaaagatgc tagatttccc aagtacggca ttaggttttt ctgctggttt
    31081 cacaggaaaa tatatagaag actatttaca gaagaaacaa atccaaacag agtttattga
    31141 agtgcctggt atgacgcgta tcaatgtatt tacacaagtc aatcaaacga gagaagaata
    31201 caaattagtg aatcaaggac ctgaaatacc gcaggcagct gtccataatt ttttaaatca
    31261 aataagagca ttacaagcag aggattatct ttgtgtttct gggagtttac caagaggaat
    31321 gtctccgaag gttttagtgg aaatcagtcg gatttgtcag gaaaaggaga ttcatttgat
    31381 tattgatagc agtgatcaag aggtcatgga ttgtttgccc tatcgaccat ttttgctgaa
    31441 accgaatgag gaagaacttt cttcttggtt cggacgaaaa atggttacag atgaagatta
    31501 ctttgtttat ggacaacgac tggtagagtc aggtgcagaa aatgttttgt tatcccttgg
    31561 tgagaaagga gcgattcttt ttacgaaaga cagggtgatt cgaggaaact cgccaaaagg
    31621 aaaagttgtc aatacagcat gtgcaggtga tgccatgtta ggcacttttt tagtaggata
    31681 catgaatcga cgaccgcttg atgaaacgtt acgtaaaagc atagcagcag gcagctctac
    31741 tgcttttcgt aaaggactga ctgatttttt ggatgtggag gaattaagta aacaaatcaa
    31801 aatcaaggaa gagaggttcg aaagatggga aaatatcaac taattgcagc aaccggttgt
    31861 ccgacaggaa ttgcacacac ctacatggcg caagaggcat tagagcaagc agctagaaaa
    31921 aaaggcatca cgatcaaagt ggagacccat ggacaaattg ggatcgagaa tgaattaacg
    31981 gctgaagaaa tcaaacaggc agatgctgtc attattgccg cagacaaaga tgtccattca
    32041 gaaagatttg cgggaaaacg agtcattgat gtttctgtca gtacaggaat caaggatgca
    32101 caacggctga ttgacgaagc attggcagga aaagggacca tcttagcaga taatgaagct
    32161 gttgatactt tagaatcaga aacgataagt ggtgctaatt ttggtcgcag catctataaa
    32221 aatttaatga atggcgtctc tcatatgttg cctttcgtgg tagctggcgg agtattgatt
    32281 gctatttcat ttgccgtttg gggaatctat tcttttgatc cagaccatgc acaatacaat
    32341 gctacagcgg cgatgttaaa aacggttggc gatgcagcaa tggggatgat ggttccagtt
    32401 ttagcagctt atattgcaga agggatcgcg aaacgtccag ggttagtcgt tggatttgtt
    32461 ggtggaatcg tggccatgag tggcggtact ggatttttag taggtatttt agctgggttc
    32521 ttagcaggtt attttatctt atttttgcaa aagctactga caattttacc taaacaacta
    32581 gatggcttga aggcaatctt cttatatcca gttattggtg tagtgtttat cggcatcgtg
    32641 atgtctttgc tggcgggtcc aatgaaggct ataaatgaag gaatgatgag ctttttagct
    32701 ggatttgaac attcgaatcc acttgttctt ggcgtgattg ttggttgcat gtgtgcgttt
    32761 gatatgggag gaccgattaa taaggcggca tatgtaacag gtacagctct tttggcacaa
    32821 ggaaacacga cttttatggc tggcgtgtca gtagcttgca ttgcaccacc gttgattact
    32881 ggttttgcaa ctttattgtt tggtaaatat tttgatacta atgatcgtaa tgcaggtttg
    32941 gttaatttta ttttaggatc aactcatatt acggagggag ctattccgtt tgctgcgaaa
    33001 gacccgatcc gagtattacc aacgatgatg ttaggctcat caattgctgc tgtcttgacc
    33061 tatctgtttg gtgtccaagt accagcccca catggcggtt ttttagtgtt acctgtagtg
    33121 acgggaaaaa tccaatgggt tacagcgatt cttattggtt cgatcgttgg tggagtgata
    33181 ctaggtttga tccaaaagaa aagagtaact gataaagaca agaagatgga taataaagca
    33241 gcaaatcgta cagttatcga agaaggaaga gcaggaggct tatcatgaca atcattcatg
    33301 aagagaacat ttttttacaa cagccattag atagtcaaga agaagcattt aagtttttag
    33361 caaagcaaac attctgttta ggaatcgctc atgacgaaaa agaagtatat cgaaaacttc
    33421 tagaaagaga aaaagaaggt actacaggaa taatgtcagg ttttgctatc cctcatgcga
    33481 agtctcaaac aatcaacgaa cctagtatga ttatcgtcac actgaacaaa ggaattgaat
    33541 ggaacagttt agatagacag tcagttgatt ttatttttgc gttatttatt ccagattcag
    33601 aagcaggaac gactcatttg aaattattgt ctctcgttgc acgatcattg atgcgaaaag
    33661 aggttacaga acagttgaga aaagcaaaat caaaaacaga aatagccaca atattaacta
    33721 aagaaatagg tgaagatcta tgaagaagat gagtatagaa aagcaggaac gtttgaagcg
    33781 attgacggat gaagctggaa ttattagcgc gttagcaatc gaccagcgtg gtgctttgaa
    33841 gaaaatgatc aaccaatata aagaagctac atcagaagac atcatccaat ttaaacagat
    33901 ggtttctcaa tacctgactc cctatgcctc agctatttta cttgatccag aatgcggcct
    33961 gcctgcagca gcgcttagag agaaagaatg tggcttgctg ctagcttatg aaaaaaccgg
    34021 ctatgatacg aaaatgccgg gaagattacc tgatttattg aatatttggt cggtgaaaag
    34081 gttaaaagaa gctggtgcgg atgcttgtaa atttttattg tactatgacg tggatgaacc
    34141 agcagagatt aatgtgcaaa aacaaatagt tatagaacgg attggttcag aatgcgatgc
    34201 agaaggcata cctttctttt tagagatcct ttcttatgat gcaactattg gcgatccgtc
    34261 ctctcttgcc tatgcaaaag taaaacccca taaagttatt gaagccatgc gagaattttc
    34321 aaaagaacgt tatcaagtgg atgtattgaa agtagaagtg ccagtgaata tgagatttgt
    34381 ggaaggatat gggcaagaga cttgttatag tcaagcagag gcatccgcgt atttctatga
    34441 acaatcgaaa gtgactgaac aaccgtttat ttttttaagt gcgggagtca atgctacgtt
    34501 atttcaagat acattacgtt ttgcaaaaca atcaggttcg acattcaatg gtgtcttatg
    34561 tgggcgagca acatgggcga agggagtggc accttttgtt accgaaggcg aagaaaaagc
    34621 gagtagttgg atgttagaag aaggaaaaca aaatattaag gaattaagta cactattaaa
    34681 agaaacagca acacctgtct acgttgatag ataagaaaaa gttgtttctt cataaaaaga
    34741 tgctgctgca attagatagc gatcaatcga tgattcaaat tatacattca cttaaataaa
    34801 aatatcgact agatttcttt ttcaaaaatc ttggtcgata tttttaattg ctaaaactgt
    34861 taatgaattt ttattacact cttatttact atctacttcc gcttctgctg ctttcctgcg
    34921 ttgcggcctt tgttatttgt actgtttttt ggttgtttga tttgttttgt tgcttgtttg
    34981 ttgtcagctg gttttgctga ttctgtcacg tcttttcttg gagtgacgac ttgttttggc
    35041 gggttttgtt tcaactcttc tgccacttgt gctttcaagc gtggtttcat cataacgttt
    35101 gtgatgaatg tttgcagaca gctgaacaca ccaccgacta cccagtaaag tgtaacgccg
    35161 gctggcgcac tgatcgacat gaagacgatc atagcaggag agacgatcag catcgtacgc
    35221 atcgtctttt tctgttcttc aggaataccg attgttgaaa tatatccttg aagcagataa
    35281 gcgacccctg caatggccac aagcaccata cttggtgagc ctagatcgat gccgtaaaaa
    35341 gatgattcac ggataccttc tgtgtagcga gccgtaaaat acaaggcaga gaagatcggc
    35401 atttggatca atagaggcag acagccgatt ccgccggtca tactcaatcc gttttcgtga
    35461 tatacttgct gcatttctgc ttgtgctgcc atttgttctt cacgtgttgt tgcagctttt
    35521 agcttttgtt gtgctgcttc aacttgtggt tttaacgctt gcattttttc agattggatc
    35581 attgtttttt tcgattgact gatacctagt ggcaagatga tgatacgaac gatcacagtc
    35641 attgcaataa ctgcccagcc gtaagaccaa ttgaaattct caactagata tgtaatcgct
    35701 tggcccatag gatggacgag tactcgataa acgattcctg aagtgtcagg gtttccgttt
    35761 gaatccatcc gcacacagcc agataaaaag agcaggatac tgacaagtcc ggatcccaat
    35821 aaccagtttt tccatttatt catagaatac ttcctcttct ataattttta cacaaactta
    35881 actatactaa aaaagcgcat ggaattcaat caacttcata gaaatttcat ataaatattc
    35941 tttctttttc gtagcgaaaa attttgaaaa aagacaaaaa atcaagaaag aaatacttgg
    36001 aaagaaaaga gtgattccaa gtgtttcttt cttatataaa aaactaaaaa cttttttgtt
    36061 ctgtctggaa aatatgggtt aagcaaattt attgcaagtt atagatcagc ttttttacta
    36121 cctatcttta atacttgagg caaagcgcct caaaaacaac ctgatcgacg gtgttcaaaa
    36181 gtcagtttct cggtattaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    36241 ttttccttaa tactcaaaac taagcgacct tttcacaacc ttatcccacc acatcaaatt
    36301 tctttcgttc ctcaatcatc ggatcttctt ggacatccac atactgcact cgtccacttg
    36361 ggctcggaga cttcttgact tcttcgataa atttttggat gatgtcgtca tttccgattg
    36421 cttcgattgt gactgagccg tcgtcttcgt tttttaccgt tccggtcacg cctaactgat
    36481 cagcaaccat cttggtcata tagcgaaatc ccacgccctg gacacgtcct tggacattca
    36541 ttttaacttt gcgcatgttc gtcacctttc tttcctgttt tataaatcaa tgataccgat
    36601 ttttttagga tgaaacaagc tcaagcacct attccatgat ataatcaggt gaggtgaagg
    36661 aatgtgaaag agatccaatc agcaaaaaat ccaaaaatca aagaattaaa aaagctacat
    36721 aaaaagaaat acagagaaga aaaggcagaa tatcttttgg aagggttcca tctgatcgaa
    36781 gaagctgcgt cgtctgatgc agaaataaaa gaactttttg tgacacagag aggtatgaaa
    36841 gaatggggag actggctggc tgtaaacaaa gagatttcta cttttctagt ttcagatgag
    36901 gtcatgaccg ctatgtcaga tctacctaca ccgcaaggga tcttagcggt gatgacgtta
    36961 acatctgaag atacgccaga tttttcagga ggctggcttt tactagataa tgtacaggat
    37021 cctggaaatg tgggaacgat gatccgtacg gcagatgcgt ttggtatggc tggtgtttta
    37081 ttaggtaaag gaacggcaga tatttatagt acaaaggtgc tgcgaagcat gcagggaagc
    37141 aattatcatt tgcctgtgtt acaaaaagat ttggcagaag tgatccctgt aatgaaaggc
    37201 aaagggttta cggtgtacgg tacagaatta aatgaagaag cacatgcatt agacaaagta
    37261 gaaaaaacag aggatttcgc tataatcatg ggaaacgaag gtcagggagt aagtcaggaa
    37321 atcttgtctt tgactgatca gaacgtatat atcccaatga aaggaaatgc ggaatcattg
    37381 aatgtcggag tcgcagcagg gatcgttctt tatcattttt ctttatgatt aaggaactat
    37441 taagctttag ctattttatc tagagcgact agaataatcc gaacactggt ggtatactta
    37501 gatcaatttc agaagaaaat gaaattcaaa aaggtggcag atcgataatt atgacagaaa
    37561 aatggcgtga agacgaagaa tatatgtctt atgtacaaga tcttttagaa acagaagcag
    37621 tgaaaaaatt agcgaattac acacagcatg ttcattccac tcgtttggag cattccatca
    37681 gtgtttctta ctatagttat ctgctcgcaa aaaaatgggg tggcaatgct aaagcaacag
    37741 cgcgtgctgg acttttgcat gatctgttct actatgattg gcgtacgacg aaatttgacg
    37801 aggggacaca tgcgtatatc catccaagaa ttgctgtaaa gaatgcagaa aaaatcaccg
    37861 atttgtcgga tcttgagcga gatatcattt tgaagcacat gtggggtgca acgatcgcac
    37921 cgcctaagta taaagaagga tatattgtga cattcgtgga caaatactgc gcagtcaaag
    37981 aagcggctca gcccatgtct gcaagtatgc gtaagagatg gcaacgttac tttgggaaag
    38041 aatcctcgat ttaacaggta aaaccaataa tagaccatat tgaggttgtg acagaagtgg
    38101 tcagcttcaa gaaataagaa ggaatttacg aaaattactt ctcaaatttt tgtgaagttt
    38161 cggcttatta ccgcagatgc aagcttttga acaccattca ttcgaaacaa gggactttga
//
LOCUS       DACIFS010000024        37191 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.024, whole genome shotgun sequence.
ACCESSION   DACIFS010000024 DACIFS010000000
VERSION     DACIFS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.024"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            647..964
                     /locus_tag="IYA83_001220"
     CDS             647..964
                     /locus_tag="IYA83_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ8586664.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IYA83_001221"
     CDS             complement(1055..1420)
                     /locus_tag="IYA83_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586665.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IYA83_001222"
     CDS             1628..2110
                     /locus_tag="IYA83_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586666.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IYA83_001223"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IYA83_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ8586667.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="IYA83_001224"
     CDS             complement(3484..4758)
                     /locus_tag="IYA83_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ8586668.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="IYA83_001225"
     CDS             5031..7502
                     /locus_tag="IYA83_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ8586669.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="IYA83_001226"
     CDS             complement(7605..9341)
                     /locus_tag="IYA83_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ8586670.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="IYA83_001227"
     CDS             complement(9338..10042)
                     /locus_tag="IYA83_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586671.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="IYA83_001228"
     CDS             10173..10676
                     /locus_tag="IYA83_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ8586672.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="IYA83_001229"
     CDS             10636..10974
                     /locus_tag="IYA83_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ8586673.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="IYA83_001230"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="IYA83_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ8586674.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="IYA83_001231"
     CDS             12525..13103
                     /locus_tag="IYA83_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ8586675.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="IYA83_001232"
     CDS             13107..13628
                     /locus_tag="IYA83_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ8586676.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="IYA83_001233"
     CDS             complement(13707..14261)
                     /locus_tag="IYA83_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ8586677.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="IYA83_001234"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="IYA83_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ8586678.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="IYA83_001235"
     CDS             14801..15052
                     /locus_tag="IYA83_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ8586679.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="IYA83_001236"
     CDS             15177..15968
                     /locus_tag="IYA83_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586680.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="IYA83_001237"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="IYA83_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ8586681.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="IYA83_001238"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="IYA83_001238"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ8586682.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="IYA83_001239"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="IYA83_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ8586683.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="IYA83_001240"
     CDS             18151..18444
                     /locus_tag="IYA83_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ8586684.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            19287..21614
                     /locus_tag="IYA83_001241"
     CDS             19287..21614
                     /locus_tag="IYA83_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ8586685.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21740..22069
                     /locus_tag="IYA83_001242"
     CDS             21740..22069
                     /locus_tag="IYA83_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ8586686.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            22109..23113
                     /locus_tag="IYA83_001243"
     CDS             22109..23113
                     /locus_tag="IYA83_001243"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ8586687.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            23110..24539
                     /locus_tag="IYA83_001244"
                     /pseudo
     CDS             23110..24539
                     /locus_tag="IYA83_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            24745..25917
                     /locus_tag="IYA83_001245"
     CDS             24745..25917
                     /locus_tag="IYA83_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ8586688.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(26079..26534)
                     /locus_tag="IYA83_001246"
     CDS             complement(26079..26534)
                     /locus_tag="IYA83_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586689.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            27124..27546
                     /locus_tag="IYA83_001247"
     CDS             27124..27546
                     /locus_tag="IYA83_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8586690.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(27712..28905)
                     /locus_tag="IYA83_001248"
     CDS             complement(27712..28905)
                     /locus_tag="IYA83_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8586691.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            29129..29506
                     /locus_tag="IYA83_001249"
     CDS             29129..29506
                     /locus_tag="IYA83_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8586692.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            29494..29832
                     /locus_tag="IYA83_001250"
     CDS             29494..29832
                     /locus_tag="IYA83_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8586693.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29966..30409
                     /locus_tag="IYA83_001251"
     CDS             29966..30409
                     /locus_tag="IYA83_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ8586694.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            30671..31486
                     /locus_tag="IYA83_001252"
     CDS             30671..31486
                     /locus_tag="IYA83_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8586695.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            31496..32158
                     /locus_tag="IYA83_001253"
     CDS             31496..32158
                     /locus_tag="IYA83_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ8586696.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            32363..34027
                     /gene="alsS"
                     /locus_tag="IYA83_001254"
     CDS             32363..34027
                     /gene="alsS"
                     /locus_tag="IYA83_001254"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ8586697.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            34040..34750
                     /gene="budA"
                     /locus_tag="IYA83_001255"
     CDS             34040..34750
                     /gene="budA"
                     /locus_tag="IYA83_001255"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ8586698.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            34880..35377
                     /locus_tag="IYA83_001256"
     CDS             34880..35377
                     /locus_tag="IYA83_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ8586699.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            35562..35822
                     /locus_tag="IYA83_001257"
     CDS             35562..35822
                     /locus_tag="IYA83_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586700.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(36184..36423)
                     /locus_tag="IYA83_001258"
     CDS             complement(36184..36423)
                     /locus_tag="IYA83_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586701.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            36709..37023
                     /locus_tag="IYA83_001259"
     CDS             36709..37023
                     /locus_tag="IYA83_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586702.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT        12497 a         6479 c         7377 g        10838 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattta aaaataaaat
    19141 ctttgtatta aaggacaaga ctaaaataac cttagaataa aaagagaaaa aaagccccaa
    19201 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
    19261 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
    19321 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
    19381 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
    19441 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
    19501 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
    19561 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
    19621 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
    19681 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
    19741 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
    19801 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
    19861 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
    19921 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
    19981 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
    20041 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
    20101 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
    20161 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
    20221 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
    20281 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
    20341 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
    20401 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
    20461 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
    20521 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
    20581 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
    20641 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
    20701 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
    20761 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
    20821 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
    20881 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
    20941 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
    21001 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
    21061 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
    21121 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
    21181 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
    21241 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
    21301 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
    21361 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
    21421 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
    21481 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
    21541 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
    21601 gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
    21661 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
    21721 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
    21781 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
    21841 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
    21901 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
    21961 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
    22021 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
    22081 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
    22141 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
    22201 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
    22261 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
    22321 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
    22381 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
    22441 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
    22501 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
    22561 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
    22621 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
    22681 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
    22741 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
    22801 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
    22861 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
    22921 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
    22981 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
    23041 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
    23101 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
    23161 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
    23221 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
    23281 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
    23341 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
    23401 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
    23461 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
    23521 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
    23581 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
    23641 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
    23701 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
    23761 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
    23821 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
    23881 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
    23941 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
    24001 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
    24061 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
    24121 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
    24181 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
    24241 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
    24301 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
    24361 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
    24421 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
    24481 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
    24541 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
    24601 tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag gaggaactat
    24661 tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc tcttcatata
    24721 catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact ggacatcttc
    24781 atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt agaagattca
    24841 ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc atcgaataag
    24901 aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca acagttggca
    24961 gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc aaaagcatta
    25021 aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat cgaaatccca
    25081 aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt gcaaaaagga
    25141 acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca tgccgttctt
    25201 tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa
    25261 ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt agaaaaaatc
    25321 caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag agatctcgtg
    25381 actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt gacggggcat
    25441 cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa
    25501 aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg cttagtttgg
    25561 ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc tttatctttt
    25621 tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc
    25681 aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc tgatgaatac
    25741 ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca tgaggagctc
    25801 ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa attgaaaaaa
    25861 ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa agaatgaatg
    25921 aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag ctgacgaaat
    25981 ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta tttttttatg
    26041 tttcagaaga attttattat tatagtataa cttttgaatt agtttccata acatttggag
    26101 caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata cgtaccattt
    26161 ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt ttgttcatta
    26221 ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc tgcttgctgt
    26281 cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt
    26341 cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa
    26401 tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct
    26461 gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat
    26521 tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct
    26581 tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc ggagatcgtg
    26641 gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt agtagcacca
    26701 cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt cactaaattt
    26761 cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt ttacttactt
    26821 gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc cgttttgata
    26881 cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata tgtacgttat
    26941 tttattatta gattattgac atttatataa taaacgatta aatgattcat atcaatagaa
    27001 tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt gtgacaatat
    27061 gatatttgta tccgtttttt ttcttgtatg tagtatagta aagtagatac aggaggagaa
    27121 aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat tcatttactg
    27181 cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa ggtatcaagt
    27241 aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata tcctgataag
    27301 gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga gaacttccca
    27361 atctacatta tttatcttca tttctacaga aaatcatata attatcattt gattgaattc
    27421 atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata tacgagtgta
    27481 tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac ggacgtcaaa
    27541 gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct gcatcaagta
    27601 atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt cttattaccg
    27661 cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact atcaatgctg
    27721 caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca gactgactag
    27781 gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca tcaagaaacc
    27841 gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga aattattcgc
    27901 cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc caccatacat
    27961 gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact ggctatgggc
    28021 taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa aggctagttt
    28081 aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca tgcctaaaat
    28141 agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat tttgcataat
    28201 tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta ccatgatact
    28261 aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa gtttggtttt
    28321 cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc gacgaatgaa
    28381 caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac caaacgttgg
    28441 aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac caataccagc
    28501 aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca aggacataca
    28561 cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat aaaccaagta
    28621 gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga ccgtttgaga
    28681 aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac ctccaatgag
    28741 catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa tatgaaaaga
    28801 agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct ctaaagcaat
    28861 tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta tacccctttt
    28921 cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa acaagacttt
    28981 tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt ttaatagaaa
    29041 tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat aagtaaagtg
    29101 gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg aagaaaaggg
    29161 catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac cagttaatcg
    29221 gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg actttacgat
    29281 ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc gcctatctag
    29341 cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa gtgaactttt
    29401 gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa aaatcaagaa
    29461 atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa atcaaggagc
    29521 atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga aggaacaatc
    29581 tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca actgaaggag
    29641 ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat gatcttgaca
    29701 gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa cgaatcaaac
    29761 cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta cgatcggctg
    29821 tgtggaagct gataattcga atacattggc actattacta gaaaagttga tccgtgatta
    29881 tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg agtgactaga
    29941 aaaaagcctg ttagatttag cgattttgct agatccagta gatacagaga aatacgaaac
    30001 gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt ctgttttagc
    30061 aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga tctcaaagcg
    30121 tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc agttgctgat
    30181 cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc aggtaggtgc
    30241 tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat tcgttccgct
    30301 tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga ttttatcacc
    30361 tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat aagtatctct
    30421 aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt gtattttttc
    30481 atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc gcttacaaac
    30541 ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt aatcattaca
    30601 aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag caaaaattcg
    30661 ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt
    30721 attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt
    30781 gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat
    30841 ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat
    30901 cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta aactaaattt
    30961 taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc
    31021 ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata cgttgtggtc
    31081 gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatactacag
    31141 tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag agtggggaat
    31201 ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac
    31261 attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa
    31321 taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt
    31381 aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca
    31441 aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa
    31501 agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt
    31561 ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt
    31621 gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg
    31681 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
    31741 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
    31801 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
    31861 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
    31921 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
    31981 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
    32041 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
    32101 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
    32161 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
    32221 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
    32281 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
    32341 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
    32401 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
    32461 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
    32521 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
    32581 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
    32641 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
    32701 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
    32761 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
    32821 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
    32881 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
    32941 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
    33001 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
    33061 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
    33121 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
    33181 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
    33241 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
    33301 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
    33361 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
    33421 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
    33481 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
    33541 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
    33601 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
    33661 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
    33721 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
    33781 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
    33841 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
    33901 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
    33961 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
    34021 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
    34081 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
    34141 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
    34201 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
    34261 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
    34321 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
    34381 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
    34441 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
    34501 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
    34561 tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
    34621 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
    34681 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
    34741 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
    34801 ttttgaggag aaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
    34861 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
    34921 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
    34981 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
    35041 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
    35101 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
    35161 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
    35221 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
    35281 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
    35341 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
    35401 aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt tttcggtttt
    35461 tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt taatgttatt
    35521 atcattttaa ataatgaaaa aacattaagg atggatgaaa tatgaataac gaaactatca
    35581 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg attggagaag
    35641 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
    35701 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
    35761 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
    35821 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
    35881 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
    35941 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
    36001 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
    36061 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
    36121 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
    36181 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
    36241 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
    36301 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
    36361 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
    36421 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
    36481 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
    36541 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
    36601 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
    36661 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt ggatttttac
    36721 agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt ttttgataag
    36781 agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga aaaaaagtgg
    36841 gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact ttcttcaaca
    36901 ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat tattggaatc
    36961 cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc aaaaaataat
    37021 taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta tagcgaatgt
    37081 gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat tttatgttag
    37141 gatgaattaa tttctctaga gtttataatt cgccgtttgt ataattaagc g
//
LOCUS       DACIFS010000025        37102 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.025, whole genome shotgun sequence.
ACCESSION   DACIFS010000025 DACIFS010000000
VERSION     DACIFS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.025"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(160..444)
                     /locus_tag="IYA83_001260"
     CDS             complement(160..444)
                     /locus_tag="IYA83_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586703.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="IYA83_001261"
     CDS             complement(462..1352)
                     /locus_tag="IYA83_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ8586704.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="IYA83_001262"
     CDS             complement(1372..1590)
                     /locus_tag="IYA83_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586705.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="IYA83_001263"
     CDS             complement(1560..3260)
                     /locus_tag="IYA83_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ8586706.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="IYA83_001264"
     CDS             3527..4300
                     /locus_tag="IYA83_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586707.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IYA83_001265"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="IYA83_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ8586708.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="IYA83_001266"
     CDS             complement(5124..6095)
                     /locus_tag="IYA83_001266"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ8586709.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IYA83_001267"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="IYA83_001267"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ8586710.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IYA83_001268"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="IYA83_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ8586711.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="IYA83_001269"
     CDS             complement(9050..9874)
                     /locus_tag="IYA83_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ8586712.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="IYA83_001270"
     CDS             complement(9847..10527)
                     /locus_tag="IYA83_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586713.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="IYA83_001271"
     CDS             complement(10550..11488)
                     /locus_tag="IYA83_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586714.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /locus_tag="IYA83_001272"
     CDS             complement(11733..12584)
                     /locus_tag="IYA83_001272"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ8586715.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IYA83_001273"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="IYA83_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ8586716.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="IYA83_001274"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="IYA83_001274"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ8586717.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IYA83_001275"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="IYA83_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ8586718.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IYA83_001276"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="IYA83_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ8586719.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="IYA83_001277"
     CDS             complement(19462..20703)
                     /locus_tag="IYA83_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ8586720.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IYA83_001278"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="IYA83_001278"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ8586721.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22614)
                     /locus_tag="IYA83_001279"
     CDS             complement(21730..22614)
                     /locus_tag="IYA83_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8586722.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="IYA83_001280"
     CDS             complement(23392..24678)
                     /locus_tag="IYA83_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ8586723.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="IYA83_001281"
     CDS             complement(24671..25939)
                     /locus_tag="IYA83_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ8586724.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="IYA83_001282"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="IYA83_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ8586725.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="IYA83_001283"
     CDS             complement(26711..27157)
                     /locus_tag="IYA83_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586726.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="IYA83_001284"
     CDS             complement(27250..27495)
                     /locus_tag="IYA83_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586727.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="IYA83_001285"
     CDS             complement(27610..28188)
                     /locus_tag="IYA83_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ8586728.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="IYA83_001286"
     CDS             complement(28265..28795)
                     /locus_tag="IYA83_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ8586729.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IYA83_001287"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="IYA83_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ8586730.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="IYA83_001288"
     CDS             complement(29652..30047)
                     /locus_tag="IYA83_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ8586731.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /locus_tag="IYA83_001289"
     CDS             complement(30044..31456)
                     /locus_tag="IYA83_001289"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ8586732.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IYA83_001290"
     CDS             complement(31453..31989)
                     /gene="cysE"
                     /locus_tag="IYA83_001290"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ8586733.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32311..33771)
                     /locus_tag="IYA83_001291"
     CDS             complement(32311..33771)
                     /locus_tag="IYA83_001291"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ8586734.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMDWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="IYA83_001292"
     CDS             complement(33941..35098)
                     /locus_tag="IYA83_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ8586735.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IYA83_001293"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="IYA83_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ8586736.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="IYA83_001294"
     CDS             complement(36565..37092)
                     /locus_tag="IYA83_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ8586737.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10250 a         8090 c         6505 g        12257 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agtcaggaaa cgccagaatt gagcattgat cttcgccaac aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggatcg gcatgaaaag atttttccct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt ttacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttaaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatc cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atctaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 aaactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgattaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ct
//
LOCUS       DACIFS010000026        36570 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.026, whole genome shotgun sequence.
ACCESSION   DACIFS010000026 DACIFS010000000
VERSION     DACIFS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.026"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IYA83_001295"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IYA83_001295"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ8586738.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IYA83_001296"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IYA83_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ8586739.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IYA83_001297"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IYA83_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ8586740.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IYA83_001298"
     CDS             complement(3743..5854)
                     /gene="hflB"
                     /locus_tag="IYA83_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ8586741.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IYA83_001299"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="IYA83_001299"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ8586742.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IYA83_001300"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="IYA83_001300"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ8586743.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="IYA83_001301"
     CDS             complement(8054..8524)
                     /locus_tag="IYA83_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ8586744.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="IYA83_001302"
     CDS             complement(8570..8995)
                     /locus_tag="IYA83_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ8586745.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="IYA83_001303"
     CDS             complement(9062..9331)
                     /locus_tag="IYA83_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ8586746.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="IYA83_001304"
     CDS             complement(9342..10937)
                     /locus_tag="IYA83_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ8586747.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IYA83_001305"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="IYA83_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ8586748.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="IYA83_001306"
     CDS             complement(14490..15050)
                     /locus_tag="IYA83_001306"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ8586749.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="IYA83_001307"
     CDS             15403..16377
                     /locus_tag="IYA83_001307"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ8586750.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="IYA83_001308"
     CDS             16547..17947
                     /locus_tag="IYA83_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ8586751.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="IYA83_001309"
     CDS             complement(18025..18747)
                     /locus_tag="IYA83_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ8586752.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="IYA83_001310"
     CDS             complement(18826..19332)
                     /locus_tag="IYA83_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ8586753.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="IYA83_001311"
     CDS             19455..21254
                     /locus_tag="IYA83_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ8586754.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..22896)
                     /locus_tag="IYA83_001312"
     CDS             complement(21322..22896)
                     /locus_tag="IYA83_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586755.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IYA83_001313"
     CDS             complement(23151..23660)
                     /gene="mreD"
                     /locus_tag="IYA83_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ8586756.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IYA83_001314"
     CDS             complement(23664..24518)
                     /gene="mreC"
                     /locus_tag="IYA83_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ8586757.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24673..25311)
                     /locus_tag="IYA83_001315"
     CDS             complement(24673..25311)
                     /locus_tag="IYA83_001315"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ8586758.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25312..25962)
                     /locus_tag="IYA83_001316"
     CDS             complement(25312..25962)
                     /locus_tag="IYA83_001316"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ8586759.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IYA83_001317"
     CDS             complement(25975..26874)
                     /gene="rsgA"
                     /locus_tag="IYA83_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ8586760.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IYA83_001318"
     CDS             complement(27039..29108)
                     /gene="pknB"
                     /locus_tag="IYA83_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ8586761.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29105..29845)
                     /locus_tag="IYA83_001319"
     CDS             complement(29105..29845)
                     /locus_tag="IYA83_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ8586762.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IYA83_001320"
     CDS             complement(29858..31216)
                     /gene="rsmB"
                     /locus_tag="IYA83_001320"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ8586763.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31216..32154)
                     /locus_tag="IYA83_001321"
     CDS             complement(31216..32154)
                     /locus_tag="IYA83_001321"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ8586764.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32147..32638)
                     /gene="def"
                     /locus_tag="IYA83_001322"
     CDS             complement(32147..32638)
                     /gene="def"
                     /locus_tag="IYA83_001322"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ8586765.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IYA83_001323"
     CDS             complement(32653..35061)
                     /gene="priA"
                     /locus_tag="IYA83_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ8586766.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35314..35619)
                     /locus_tag="IYA83_001324"
     CDS             complement(35314..35619)
                     /locus_tag="IYA83_001324"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ8586767.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IYA83_001325"
     CDS             complement(35624..36238)
                     /gene="gmk"
                     /locus_tag="IYA83_001325"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ8586768.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7989 c         6707 g        11988 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttgttgaaga
    21841 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg ttgattcttc
    21901 tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt caggcgctgt
    21961 tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag attgtgtagc
    22021 tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg ttgctgagct
    22081 ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc
    22141 tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac gttgttgttc
    22201 acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt cagctttctt
    22261 gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca aatcagcttg
    22321 tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag ctaattcttt
    22381 tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt gtttttgttg
    22441 ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc gtccaatagc
    22501 atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg tgttgcttac
    22561 ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac gttcttgtaa
    22621 atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt gttttgctaa
    22681 taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat cgatttgtga
    22741 ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg
    22801 agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca aggtcattga
    22861 acataccatt actgctgata ttaaactctt tttcactctt cattcctccg actggcttaa
    22921 tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt gtaccatagc
    22981 agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact tttaaaaatg
    23041 acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct agcatttctt
    23101 aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa ttattcatca
    23161 ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt caacagcagt
    23221 gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa tttaaaaatc
    23281 aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat actgaaaaat
    23341 tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca tacaattaaa
    23401 gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata gatagccccg
    23461 agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca acataataat
    23521 gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc ccctagcatt
    23581 cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag atagtacttc
    23641 attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat cgtcacaact
    23701 gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag cccataacta
    23761 tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt ccctccaaga
    23821 ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa ttgggtcacc
    23881 actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc gccattagcc
    23941 gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag taattcgatt
    24001 ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg cgacatgact
    24061 gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat gtcctgccaa
    24121 ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt cagcgtctga
    24181 ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt gttttgctgt
    24241 gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc ataagttgaa
    24301 aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc agagattgtt
    24361 ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg ctttccttca
    24421 ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac aataaccaag
    24481 gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg aattaattag
    24541 actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta
    24601 agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca aattttttta
    24661 tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg aaaaagaaaa
    24721 ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt ctacttcttt
    24781 attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag tcaagcagca
    24841 ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag gcaaataata
    24901 agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt tgaagcgagg
    24961 ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg tagctccaat
    25021 caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta actgtgtatc
    25081 tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa atacttcctt
    25141 ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc agatctactg ggtagcctgc
    25201 ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt tggacagcag
    25261 agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca tttagtctaa
    25321 tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga tatatgatcc
    25381 tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg gaacgattcc
    25441 gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag cgagttccgt
    25501 aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat tgacagtcat
    25561 gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg gtgttcctgg
    25621 attgataact actccagctt taactcctaa gctttttgtc atttgaagtg cccgatgaat
    25681 atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg caaatgctgg
    25741 aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt tagtaacagg
    25801 acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatt ggccatccat
    25861 tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt ctctttgtaa
    25921 attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt cctcctattt
    25981 tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt gtaagaattg
    26041 caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat gttttacttc
    26101 gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag aagcttctac
    26161 aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct
    26221 aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca catgacgtgt
    26281 tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat tcagttccgg
    26341 tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa agaccgatag
    26401 ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg gtacaatcac
    26461 cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg ggtcgtctga
    26521 caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca aggtaactag
    26581 aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga caatgattcc
    26641 aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca agatttccag
    26701 aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga caagcggcgt
    26761 gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc cgtttgcgta
    26821 tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg ccacctccgt
    26881 ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa
    26941 gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat
    27001 caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact ttctgaggtg
    27061 ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct tgtgctactg
    27121 gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga agacgctgga
    27181 tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt cgttcctgcc
    27241 cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc ggaagtctca
    27301 tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct ttttgtatag
    27361 ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt gatgtaagtt
    27421 tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc tgatggtaca
    27481 gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg tgtaacaagc
    27541 caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac tgtttctggt
    27601 cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt atcttctgat
    27661 ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc ttctttttga
    27721 atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc ataactttta
    27781 ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag agtgacttcg
    27841 cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt accttcttcg
    27901 attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc ttttgcgagt
    27961 gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac ttctcctcgc
    28021 ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat agctaatagg
    28081 atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc ttcctcctct
    28141 ggcaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa tgaatcaggc
    28201 attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat cacatgaggc
    28261 tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc atcagacatt
    28321 tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt
    28381 tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc atcttgaaaa
    28441 tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc cgtcaacatc
    28501 tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat acttcctctg
    28561 gcctgctcag gggacagata atgaacagat cccagcatcg tattcgtctg cgtgatcgat
    28621 gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc ttcattatcg
    28681 atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg agctaaagaa
    28741 atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac aggataatgt
    28801 gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag atactgcatg
    28861 ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag ttctgttgct
    28921 gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt
    28981 aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa tacatttgcc
    29041 ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt tttgcccaac
    29101 tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta tccgctccgc
    29161 ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg ccttcttgat
    29221 tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct gaacataaca
    29281 gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact gttccaggca
    29341 tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt tcttcgctga
    29401 tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat tgcatgattt
    29461 tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg aaccagtcat
    29521 ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc tgttcttgtc
    29581 cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat tgggcgattt
    29641 tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt accgccatct
    29701 gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca gcaagcttga
    29761 gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt cgtcttccta
    29821 catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc aaacaacaaa
    29881 tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc tcttcgattg
    29941 atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg tgtctttcca
    30001 agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg ctataagtca
    30061 gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat tggatttttg
    30121 gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt cgccgaagca
    30181 gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct gcttcgaact
    30241 gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag acacctagac
    30301 gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa gcgaccactt
    30361 ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg
    30421 cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct acaagcatcg
    30481 aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa gctaaatgcc
    30541 cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg atctcttctt
    30601 cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact cgggcactag
    30661 catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat ccgatttgat
    30721 ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt ctttcgatag
    30781 gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc aagacaccat
    30841 ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc gcctcgttta
    30901 aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa agataaagaa
    30961 gcgtcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca acgtaaaatt
    31021 ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa cccacatctt
    31081 tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag taggcgccac
    31141 ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt ttcatttttt
    31201 tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac cgcttccgtt
    31261 aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat tgatggctaa
    31321 gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga tgatcgtacc
    31381 aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc agcgtatgcc
    31441 ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt tgtcaatttc
    31501 ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt
    31561 tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg gcaatgtttc
    31621 tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta catcatcttg
    31681 tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt ttttgatcat
    31741 ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat gaacaggcgc
    31801 acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta attttggtac
    31861 ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca catcaggcac
    31921 caattcaatg atttcttcca tttctggcga accggagatt ttctctggct gcagcacacg
    31981 gataccgtgc ttcagagccg cttcttttac cggtgtcggc gtaattactc gtttccgccc
    32041 aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt ctatcagccc
    32101 ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag tcatcaagat
    32161 gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt ttctcaatga
    32221 acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa tagccaaaag
    32281 ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact tcatctgctc
    32341 gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca aggctttcac
    32401 cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca tctacttcga
    32461 tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt ccgtcattcg
    32521 caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg attacatcga
    32581 ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga tagcgcataa
    32641 tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa aaagaccttg
    32701 tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa gattcggttc
    32761 atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca aaatcatgct
    32821 gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct gattggcaat
    32881 ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta ttttcacagt
    32941 aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct gataaaactg
    33001 ctcataattt tgttttttag ctaattctat ggcgtaatgt tgaggattga atgtttggat
    33061 cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg taagcaattg
    33121 gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag cattcaacac
    33181 cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg tccctaataa
    33241 tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg
    33301 agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc ggagttcctc
    33361 ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac aattaggaca
    33421 gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac gtgtatccat
    33481 atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac agtccctaca
    33541 catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt cttttttgtc
    33601 taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg
    33661 aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg ctttggctcg
    33721 gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg attctaaaga
    33781 gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc aaattgctag
    33841 atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc
    33901 gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac
    33961 tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac cttgggaaag
    34021 tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg
    34081 cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg acaaaacatc
    34141 agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct ccagcaaaaa
    34201 gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat ttttttgttc
    34261 tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag ggtcgcgata
    34321 acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca agacagcagt
    34381 actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt cagataaaca
    34441 caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt cttcttcgat
    34501 ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat ttcgtgtatg
    34561 gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt gagaaaggct
    34621 gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca atacctgaac
    34681 ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca tcacactcgg
    34741 aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca tataatcagc
    34801 taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa taactgcctt
    34861 caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc cttggacatg
    34921 acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag ctgtttccat
    34981 ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta cgtccacaat
    35041 gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc ttatcaagcg
    35101 acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc ttctgccaaa
    35161 tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca
    35221 cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc tgggtttgct
    35281 tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt cacggatacg
    35341 tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt cttccatctt
    35401 caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg catcgatttc
    35461 ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag gctgtgcact
    35521 tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt ttgaatttac
    35581 acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc tcctttaaca
    35641 ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca ataatgtttt
    35701 tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg taaagagcca
    35761 tcatttcgat ttcttctttt gcaactttca ttcgttcttc gattacttca tctgcatctg
    35821 ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc aaaaagatga
    35881 atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca atctccaaga
    35941 aaacatcttt tccttcatcc aatgtcttgt tgatatatga caaaggagtt ccataataat
    36001 tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat tcaaactctt
    36061 ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag
    36121 tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct tttcgtactg
    36181 tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc tctgacatga
    36241 cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt ttttctgcaa
    36301 ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc tgtaccaaga
    36361 ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa acagcttctc
    36421 atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg ccgtgtataa
    36481 gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga aaatagtttc
    36541 tcacattttt gcataaattt ggcttaatac
//
LOCUS       DACIFS010000027        35565 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.027, whole genome shotgun sequence.
ACCESSION   DACIFS010000027 DACIFS010000000
VERSION     DACIFS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.027"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(401..718)
                     /locus_tag="IYA83_001326"
     CDS             complement(401..718)
                     /locus_tag="IYA83_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ8586769.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYEMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(719..973)
                     /locus_tag="IYA83_001327"
     CDS             complement(719..973)
                     /locus_tag="IYA83_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8586770.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1233..1451)
                     /locus_tag="IYA83_001328"
                     /pseudo
     CDS             complement(1233..1451)
                     /locus_tag="IYA83_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3264..4304
                     /locus_tag="IYA83_001329"
     CDS             3264..4304
                     /locus_tag="IYA83_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ8586771.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SSPSTITKDWLE"
     gene            5145..5477
                     /locus_tag="IYA83_001330"
     CDS             5145..5477
                     /locus_tag="IYA83_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ8586772.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            6096..6437
                     /locus_tag="IYA83_001331"
     CDS             6096..6437
                     /locus_tag="IYA83_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ8586773.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6456..6746
                     /locus_tag="IYA83_001332"
     CDS             6456..6746
                     /locus_tag="IYA83_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586774.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6938..7171
                     /locus_tag="IYA83_001333"
     CDS             6938..7171
                     /locus_tag="IYA83_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586775.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7577..8248
                     /locus_tag="IYA83_001334"
     CDS             7577..8248
                     /locus_tag="IYA83_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ8586776.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8301..10280
                     /locus_tag="IYA83_001335"
     CDS             8301..10280
                     /locus_tag="IYA83_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ8586777.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDT
                     AGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASL
                     HGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLK
                     VDSGLFGTGIKATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKS
                     DKEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKAS
                     VPMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            10295..11047
                     /locus_tag="IYA83_001336"
     CDS             10295..11047
                     /locus_tag="IYA83_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ8586778.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11063..11824
                     /locus_tag="IYA83_001337"
     CDS             11063..11824
                     /locus_tag="IYA83_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586779.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            11837..12097
                     /locus_tag="IYA83_001338"
     CDS             11837..12097
                     /locus_tag="IYA83_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586780.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12094..14184
                     /locus_tag="IYA83_001339"
     CDS             12094..14184
                     /locus_tag="IYA83_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ8586781.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            14211..14675
                     /locus_tag="IYA83_001340"
     CDS             14211..14675
                     /locus_tag="IYA83_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586782.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14672..14914
                     /locus_tag="IYA83_001341"
     CDS             14672..14914
                     /locus_tag="IYA83_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586783.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14932..15234
                     /locus_tag="IYA83_001342"
     CDS             14932..15234
                     /locus_tag="IYA83_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586784.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            15304..17367
                     /locus_tag="IYA83_001343"
     CDS             15304..17367
                     /locus_tag="IYA83_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586785.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            17367..19976
                     /locus_tag="IYA83_001344"
     CDS             17367..19976
                     /locus_tag="IYA83_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ8586786.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            19973..22369
                     /locus_tag="IYA83_001345"
     CDS             19973..22369
                     /locus_tag="IYA83_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586787.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            22421..22753
                     /locus_tag="IYA83_001346"
     CDS             22421..22753
                     /locus_tag="IYA83_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586788.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22754..23347
                     /locus_tag="IYA83_001347"
     CDS             22754..23347
                     /locus_tag="IYA83_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586789.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23510..25204
                     /locus_tag="IYA83_001348"
     CDS             23510..25204
                     /locus_tag="IYA83_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ8586790.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            25892..27769
                     /gene="ltrA"
                     /locus_tag="IYA83_001349"
     CDS             25892..27769
                     /gene="ltrA"
                     /locus_tag="IYA83_001349"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ8586791.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            27900..28277
                     /locus_tag="IYA83_001350"
                     /pseudo
     CDS             27900..28277
                     /locus_tag="IYA83_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            28277..29482
                     /locus_tag="IYA83_001351"
     CDS             28277..29482
                     /locus_tag="IYA83_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ8586792.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            29498..30142
                     /locus_tag="IYA83_001352"
     CDS             29498..30142
                     /locus_tag="IYA83_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586793.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            30153..30959
                     /locus_tag="IYA83_001353"
     CDS             30153..30959
                     /locus_tag="IYA83_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586794.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            30982..31212
                     /locus_tag="IYA83_001354"
     CDS             30982..31212
                     /locus_tag="IYA83_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586795.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
     gene            31330..32289
                     /locus_tag="IYA83_001355"
     CDS             31330..32289
                     /locus_tag="IYA83_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ8586796.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERLVS
                     CGMRTLYRLFSKGIFEIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQCPLNENSNGILRRNGLPKSMDFRE
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            32783..33070
                     /locus_tag="IYA83_001356"
     CDS             32783..33070
                     /locus_tag="IYA83_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586797.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            33088..33510
                     /locus_tag="IYA83_001357"
     CDS             33088..33510
                     /locus_tag="IYA83_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586798.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            33530..35122
                     /locus_tag="IYA83_001358"
     CDS             33530..35122
                     /locus_tag="IYA83_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586799.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            35378..>35565
                     /locus_tag="IYA83_001359"
     CDS             35378..>35565
                     /locus_tag="IYA83_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586800.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        13143 a         5506 c         7021 g         9895 t
ORIGIN      
        1 ccatagatat attggacttc ctcttttaaa acacaaaaat aaatactacc ttatttaggt
       61 agtatttatt aaaagttacg tcaattttga ttaaaaatat actacttgaa cctctcatga
      121 gtaaagtcac gaaatttttg ggaatagagc gtacttgata tcttattttt tcactaatag
      181 cagtgtctct tattcaccaa gcgatccccg tagttcctac ggttcttaat ttttctctag
      241 actaatgatt gtatctttag accttcggcc ataacatgga gcaaaataaa aaggtactaa
      301 gtcttatata tcaatcttcg agtaaccgat ggttaacaac catatcgagc ggcattaatc
      361 acccacctgt agaggatgag cgacttttgc ctaattattg ttaaaagcct agtttcattt
      421 gtttagattg gaaaatacgt cctacaagta tctctttttt ttcatggtcg atccgataaa
      481 ataaggcata gcttttgccg atcacgatcc gatatgttgg gatatccatt tcatagattt
      541 cggaaacttc ctcatgcata gttggaaaat ttttggttag ttctaaagcc ttctggatcg
      601 cacctacaaa tctttttatg ctggctggag aaagagcaag ctcattttcc cattcttcta
      661 ttatcgtttc aaggctggtc tggaaacttt tagaataaac tagcgtgtac tctttcatct
      721 attcactcca gccgtatttt ccgaaagctt ttaccatttc aatttctgaa actgttaaac
      781 ctttctcaaa gtcttggaaa ctttcttcaa tcaatttgcg atcgtgttct tttgctattt
      841 gagtagtatc taatatgaat aagttgcctt cttgtttggc ttggatagtg tctccgcttt
      901 ttaatccaag agagcttgga aggatcactc ccacgctatt gccgatcttt ctaaccttta
      961 gttcttttcc catgttatca cactcctttt agtttttgat aaataaatta taacatgtta
     1021 taacaacaat tgccaacggt gtttcttaag ggattccact ccatattatt attggacaaa
     1081 ataagccact tagaacgctg aaaacatata caaacgatat caaaatacga gtgaactact
     1141 caccatacac ttccgttaac atccatttaa gtaggtattt ttattatata tagatttaac
     1201 atatgtcggt cggttgattg atatagctaa agttataact caaccgaagc cgatttatct
     1261 acacgaataa agttgcgagt gttctcggct aattcataaa aattgttatg tgttccatat
     1321 agcaatactg gaataacttt attttcttga atctcagaag caactctata cacggtgacc
     1381 gcatacttaa atccattaga ccaattacct gataaacctc cagttttttg ctcttactta
     1441 aatggatcaa tagaaatttc atcacaaacc tatagaggat gagtgacttc tgcctaatta
     1501 cgttaaaaaa tattgaaagt aactctatgt agcaaaaaat atctagaatt tcatttcctc
     1561 aaaactgatc gatattgtat ccaaaaatat atactaccta aaataggtag tatatattaa
     1621 aatatgtgct tttgttagaa aaatcaatac ataaattatc atatctgtat atgtcattta
     1681 ggtattttag ttatgttata atagttttgc tgtctgctac cccggcctaa tctggcaagg
     1741 ggaggaggag atataatgaa ataccttttt gattttgttt ttgcaatcaa gccatttgca
     1801 gaggtaactg cacactacat ctgcaagtgg cttgattgca aaattgccaa aaaagacagt
     1861 aagcctaccc gttaattcta taatatagaa acgtaaaaaa aaaccccatc agcctgcacg
     1921 ctgatggggt ttggtgatat aatgaaatac ctctttgtta ctcataatta taccataatt
     1981 agtcacgaat gcaaccaata gaaaaaacta cttagaacgg tagatcatcg ctaataacaa
     2041 tgcattgtcc accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg
     2101 cttttaacta ggcctctccc cttctattat aaaagaagat atatatcatt tcttaattac
     2161 ctactacctt aaaccaaaat ttaagtacta cctaatttaa gtagtactta aataatctaa
     2221 aaaatagtta tcccaattgt tatgatttta taatacgatt atttgtaaca aaatgaatat
     2281 tatataatta aacactacca tatttaggta gtgtttaatt attagcaaaa caaaaaatat
     2341 ttattctatt ctcttaactt caactaagtt atgctgcaca tctttatata aatcatatgc
     2401 aactcattag ctagacaaac tacaagcatg tagttttatc tgttaattat gtactaccta
     2461 aataaagtag tttataatta acaagtatat atatttaaaa atttaaaatt aattttttta
     2521 tgacttgtac aaatttatct gatattctct tattaagaac tacctaattt gggtagttct
     2581 tattttttac attttaagta gtatttaatt gcaatttggt agtatttttt tattatctta
     2641 ttcctagaac ggtagtactt aatataaata taggtagtat ctaatttaag tattaatggt
     2701 agtatctaat ttaacaggta gtatgtaact actttaaagt aatttttttc agattaacac
     2761 agtagtatct tattaaataa gttaattcta aaggtagtac ataattatta tatatatcta
     2821 acattggtag tacttattgt attttttagt agtatataat ctattgtttt ctagcctaaa
     2881 gccttgtctt ataagaataa tataatatat actactgaaa taaggtagta tatattacta
     2941 gtagtggtag tatgttttat atgcataagg tagttcttaa tttatttttt gttttgatac
     3001 tggtagtact taattatcct taattttaaa accaaatttt attcatacgt tcttctggta
     3061 gtatttaatt ataaataata tttatttaaa gtagttctta atgactttaa aggtagtaca
     3121 taattcattt tatttcttat tttaggtagt atttaattaa aaaacagttt taatgcgttt
     3181 gcatttttta tttatctttg ctattttcga aaagagtagg taattttaca taaataaaac
     3241 aacgtgaaag tagaggatct acaatgactg attttaattt ttttaaggcg gatagagttt
     3301 acaacgaatt attttatcaa tttccaaaag tctttattgt ttctgacgaa tacaaaaaaa
     3361 tgaaagattc aactaagatt gcctatatgc ttttaaaagc aagattagag atcgcaatca
     3421 gcaaacggca aatcgatgaa gaaggtaatg tttattttac ttatacgaca aatgaactat
     3481 gtagcgtatt aaactgccaa aaacaaaaag cgatagcaat caaaaaagag ttggaatcct
     3541 ttggtttatt attacaaaag cagatgggat ttaacaaaca gttagggaaa aataatccta
     3601 atagactata tctagcagaa ttaaaagtct cagaaaatga tatctactta ctcgaaaaat
     3661 ttgatagaga gaatagggaa aatgttgata aatcagaggg tatgaaaatc atacccaccc
     3721 tcgacgaaaa atcagacgct gaatcccttg gggctcaaga gggtatgaaa atcataccgt
     3781 gccaaaacgt tgataaatca gagggtatga aaatcatacc agaacttaat aataatatat
     3841 tagacactaa tagacacaat atagacactg aaaaagaccg cctacaagat caattgttgt
     3901 tagacaattt tgagacaatt atgacaaacg acagcattgc tacgtttgtc cctgaacgag
     3961 tattaaattt gataaaaaca ttttcttcaa gttacagtga agctcaaaaa accgtccaga
     4021 ctattcataa tgcaaagaaa aaagctgaaa tagaaagtgg tatttcgata gtttttgaag
     4081 aactcgatag ttattatgtc aatgcagaac aagaattata cacgacactg ttaaaagcct
     4141 atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt gtctatgtaa
     4201 aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa cgtttgaatt
     4261 ctgaaagctc cccaagcact attacgaaag actggttaga gtgaaacttt actgacaaag
     4321 tgattgttgc ttcatggagt aacattaaag ttaaaaattt tttattattc taattatatt
     4381 acgaatatta caaaataata gctattttaa tagaatttat aagttaaaat attatgataa
     4441 aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag aaataattaa tttcaaatat
     4501 aaaaaaggga acgtatgttt gcatactcct tttttttgag ttataataat acgcgaagag
     4561 agataatttc attatttaaa caaactaaaa atcttgatat tacttctttt caggcaatac
     4621 gaatattatt cgcttacatt tttttgtata aatttagttg ttttaaattt aattgtctgc
     4681 tatgcctgtg atgaattgat tttcgttctg cacttttgga cgataaattt ggacaattga
     4741 aaatacaaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
     4801 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
     4861 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
     4921 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc
     4981 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg
     5041 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
     5101 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
     5161 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
     5221 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
     5281 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
     5341 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
     5401 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     5461 ggcgtttaat cagctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     5521 ttgaaaatat tgtcgagact tctaaaagaa cttagaatca ttagaaaaat aaggagtaga
     5581 cttgatctat caacaatttc cttcggggag tgacggggta gatttttacg attggtaacg
     5641 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     5701 aaataatata cacagagtaa acaagaggga atttagaggt tagacaagtt agaatagcgc
     5761 ctagattaat catgagcagc tactaaggga tctaggcttt gacgcaaact catggaataa
     5821 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     5881 atgtagatac ttgcgaggac gttggtcatt gggtcaatcg agcatttacc gctgtctact
     5941 agggtttctc gtttcggacg ccataaacga gtcgatattt tttatgtatt tttaagtaaa
     6001 cgaaaaacga taaaatatga acgagtcctg aataaagtga aaaaattgat ataaaacata
     6061 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     6121 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggttttgtga
     6181 cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattaaac gaggatgatt
     6241 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     6301 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     6361 aagatacttg gtcatatttg agtgattata ttgtcagcgt tgaaatcatt gaatttgtag
     6421 cggataaaga agaataaaaa ctggaggaat aaattatgaa atttttcgaa gtaaaatcac
     6481 catactatta tgcgttaatc gcagcaaaga atgaagaaga atgtgcagaa ttgtatgaac
     6541 aagtagtaac agatatcgaa aatcgtgaag aatttattcg atgtttgcat gagttaaatc
     6601 gaaatgaagc aattcaagag gatgcgaaaa aaattagtga agaaacgtta caaccaatcg
     6661 gtatggagga agctacaaaa gacattttta caattattga tgaacaaaaa tcttgtgtat
     6721 taagcattga ttctacattg gtttaataga tccaagattc tataattttt ttcaaagtaa
     6781 aaagaaatta ggtaatgatt ctcagaccaa ttacttagga aaataaccat acaaattaga
     6841 ttttacagat aaaggattag aaggaatcac ggcgattaat aaaaaggtag tattacctta
     6901 ggcattgagt aaggaattta tttaggagga aaattagatg gcgcgaccag ttttagatgc
     6961 gatggatatg gatactttga caaaaggaag gtatgtacag ctgttagctc gtggttattc
     7021 acctaaagat gtattcaaag ccctatctaa aggaacagat atggaaaaag aaagattacg
     7081 aaaagaattc gactattgga gagagcataa cggaataaaa gatttaaaac cagcacgtcc
     7141 agttattaga cgaaaaaaat tgaagaagta agaccaaagc gtatggcgta cataggcttt
     7201 tttattttga taaaaataat gaaaggaagg aaaactcatg aattgtaaag aaatcgaaaa
     7261 tagaaaaaaa gtgtcgaaag aaatggaaga aaagttacta aaaacaatga aacaaaaaca
     7321 atgaaacaaa aacaccttaa aagattatct gtcgcgcaat atatcaatga tatgcagtta
     7381 acaggaaaag agaaagcttg tttgttaggt tcaatgaaaa actttgaaca gttgagacga
     7441 acttatgtga aaaaaagttc taattgtcaa ctattgttag aagtctcatg acattcatta
     7501 atagaattca tttttaaaac tttttctatg gaaatgagaa aaagtttttt tgttttgtct
     7561 gaaaggaggc agatggatga aaaaatggtt atttggtttt ttaggagtcg ctttaatcgt
     7621 cttttgcagt gtgtttggct atgttagcta ccaaaaacat gaaggagagg tgtttaagca
     7681 aaatatagaa aaaaagatgc ccatagatca aatcaatgaa catgcaaaga gttataagga
     7741 ggatgcaacg aacgttaata atgatatgtc tttgggacaa atgttgtcca tacaaaaaga
     7801 agcaatcgaa atgggcgtca ataaacaagc ctttgcacaa attcagattc ctgcgcttgg
     7861 tttaacattg ccgattttta aaggtgcaaa tcaatacaca ttgagtcttg gtgcggctac
     7921 ttatttttat gaggatgccg aaatgcgcaa aggaaactat gttctagcag gtcataacat
     7981 ggaaattcca ggtgttttat tttctgatat tcaaaagttg tcactagggg aggtgatgga
     8041 cttagttagt aacgatggcg tctatcgtta caaggtaacg cgtaaattta tcgtacctga
     8101 gtactttaaa ttaatcgatg gcgttcctga agagaactct ttcttgtctt taccaaagaa
     8161 aggagagaaa cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag
     8221 atacgtggta cagggagagc tacaataaca aggttttaat caacgtgagt cgttgaaata
     8281 tgaaacgagg agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgcttg
     8341 ttggaatgtt aggaatcatt gcaggctgct ttcagggata tcgagcatat gcagaagatg
     8401 tcacgcaaaa aacgccacca gagaaggtca atatcacggt tcataaatta atgtatgatc
     8461 aaggaacgca attgaatgtt gatgttgacg ggatcaaaaa cgatggttat acgcacgatc
     8521 aatatcctac gggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt
     8581 taggaaatat tacggatcaa gcattaccga gggaagattc tgatctaacc aacgcgaaag
     8641 tggacgaaat cgttaaagat gtagaagaca agggatctaa cagtgagtac gtcaagaatg
     8701 caaccaacaa aattacttca gctgtagatg aaaatggtga gattactttt gctgatcaac
     8761 cagcctatgt caacaattcc aaagggaatg tatatgtggt ttatgaaagt aaatccgctg
     8821 ctggattagt cacacaaaaa gcaaaaccaa tggttgtgat tgcgccaatg actgataata
     8881 caggttcagg ttttttaaaa gacattcact tatatcctaa aaatattgtt tctaagttga
     8941 gttttgaatt aacgaaattt ggtgacgatg gcacagctca aagcaaacaa acacctttaa
     9001 aaggggcaaa atttgagtta tacaagggcg aacctggaaa aggaacaaaa ttaggagatc
     9061 ttgtatcgga tgatcaagga aagctgactg ctactgattt gacgttagga aaatactatt
     9121 ttgtggaagt tccttcagaa gttgtagtgg gatcagataa agaaccaaca gccgatcaat
     9181 atttattagg cgcagacgct cgcaacgatg cgcacaataa attaaccttt gaaattacga
     9241 atgatggcgt gacaagtgac ttaaaagctt catacgtaaa ctataaagca ccagtgatcg
     9301 ataaaacagt aacaaatgga acaggacaag aacattcttt ccaaattggt gacgcagtaa
     9361 attatcaagg aacgattcat atcccaacag acattgcagg cggagcagac ggtatcacag
     9421 taaacggagt gaaaagcgaa acttctcctt acagtgtatt taaatggggg gatacagcag
     9481 gacaaggact ttcttatgtc gctgctaaag caaatatcaa agtaacaaat aaagatggat
     9541 ctgtggtatt aaaagaaaat acagattata aaatccaaaa ttcagaaaat ggctttgtga
     9601 tcgattttat cgtgaataat ggccaagtaa gtgatactgt agcaagttta cacggacaag
     9661 atcttcaaat gacttataac atgtatgtaa atgacagcgc agctgttgcc aatccattaa
     9721 caaatagcgt ggatttcgtt tataacaaca atccatttaa tcaggaagaa caccacgaaa
     9781 aaacgaaagc tgacgttgtg acttatggtg caaaattctt aaaagtcgat agcggactat
     9841 ttggaacagg aatcaaagca acaccgcttg aaagcgcaga atttgctgct aaaaacgcag
     9901 aaggaaaata ttatggtgga ttagttgata cggataaaaa cggtgtcaaa gaagcagtat
     9961 gggtagacga tgtagcaaat gcagcgatcc taaaatcaga taaagaaggt cactttgaaa
    10021 tcactggttt aactgaaggt gaatattcac tggaagaaac aaaagcgcct gaaaactatc
    10081 aaaaattaac gaaagagatc tctttcaaag tagacaaaga ttcatacaaa gaagaaaacc
    10141 gaattacgat caaaaacaat caaaaagctt ccgtaccaat gaccggtagc aacggttttc
    10201 aaacgtatgt attaatctct tgcttgttgc taggagcagg ggcactttct gcagttgttt
    10261 actttaagaa gaaagcctag aaggatcaaa accaatgatt atcaggaagc ctaagaaaaa
    10321 gcgaatcatg ggtaaatgga tcattgcttt ctggttactt tcagctgtag gtgtcttact
    10381 tttgatgcct gctgaagcga gcgtggcgaa ataccaacaa aatcagcaga ttgccgcaat
    10441 cgatcgaaca ggaacggcag cagaaacgga ttcttctttg gatgttgcta agattgagtt
    10501 aggcgatcct gttggtattc ttacaattcc aagtatttct ttaaaactac caatttatga
    10561 cggaacgtcc gacaaaattt tagaaaatgg tgtaggtatt acggaaggaa ctggtgatat
    10621 tacaggagga aacggtaaaa atcctttaat cgcaggtcat agtggacttt ataaagataa
    10681 tctttttgat gatttacctt cagtaaaaaa gggagaaaag ttttatatca aagttgatgg
    10741 tgagcagcac gcttatcaaa tcgatcggat tgaggaagtg caaaaagacg aactacaaag
    10801 aaactttgtc acttacttag aaccaaatcc caatgaagat cgagtaacgc taatgacatg
    10861 tacgcctaag gggatcaaca cacaccgttt tctagtgtat gggaagcgag taacctttac
    10921 aaaatcagaa ttaaaagatg aagaaaataa gaaacaaaaa ttatcttgga catggctttt
    10981 aggaagtact gtttttcttt cagtcatgat cattggttcg ctatttgttt ataaaaagaa
    11041 aaaataaagg agggtaacca acatgagaaa tagaaaatgg ctatttgttt gtgcagccgc
    11101 tcttttttct ttcactgcct acacacagtt gacgtttgca gacagacaag aaagcacgga
    11161 tagcacagaa caaacaatcc aaacgtcaga tagcagcacg attcaaagca gcgatgaaac
    11221 cgtcgattcg tcaacagttg attcttcaga agaaccgaaa attgctgaag acgatcaaat
    11281 agtcaaacag accattcacg ttcaaaaaat tatttcttcc gaacaattag acaaccaggg
    11341 gcaaaaactt gaaaaacaag aaggagtaaa tggcgcgaag ttttctgttt atgatgtatc
    11401 ggacatttta caaaagatgg atgttaagga tctcactact gaccaaattg aatcccaatt
    11461 aaaagatcga gtgaaaaaac tattcagtga tcaattgaag ctagtttcta atggaaaaac
    11521 aaagacaatt gatcaacaaa caggtgtctt tgaattctcg atagaagtac aggcaaatca
    11581 gaaacaagct tattatattg tcaatgaatc aagtcctgaa aacatttcta atagcgagga
    11641 tattttgcta ttaacacctg tttctgataa aaacggtgaa ttcttaaagg atgtgtggat
    11701 ctatcctaaa tcagaagctt cgcaacctaa agaagaagta aaaaaaatcg tttctactgg
    11761 cgtaaagaaa aacttctttg agaattgttg ggattttgtt actcacttgt tttctagaga
    11821 ttaaggaaga aataaaatga aactgatcaa attattatta ttcaattttt cttgtttaac
    11881 cgcagtcttt ttgtactgcg gtttattatt ttctaaacta tctattttgc caagctacca
    11941 actataccta atgaggattt taaacatacc agctgcttcc atgccgctag atattatgga
    12001 aaagctcatt aggaatccat tatttactac gatcaatttg atcgttattg tcggaatttc
    12061 actagtcatt tataaaaaag aggggatgaa gggatgaatt ttaaacactc gtggacaaga
    12121 cggttaaaat atgtttttcc cataatgatg atgttaggta cattgtttgg gttaaaaaca
    12181 gccaatgtct ttgctgaaaa agttattgtc gatccgtcaa atccaatcca gaacgtaaag
    12241 aataatttta ttattccaaa agatgttcct aatgcaaaaa caaatattac tgtaaacgga
    12301 gcgtcaaaag tttacaaata tcatgttgat gccaacaaag gtggttttac agacgactac
    12361 gtaggattag tggagggaaa caaaaatata cgctatcctt ctttccaaaa agaatacggt
    12421 gaaacaaacc ttgttttaga aggcgtaaca accaacacca aagtcaatat tgactatgga
    12481 aaaatcggta cgtataacgg gaaaaaagtc aatatcaagc tagttctatc taatattcac
    12541 ttatactcgg atacgttgcc gtggaatcta ctagataaca actatacaaa aactcatttt
    12601 cgagacgatg gctataaaaa tacgaatggt gcaatgagta aatctaaaaa aagaacagta
    12661 ctttggatct cagacaatct tttctcagga attgtttacc attcaacaca aatgaatgtg
    12721 cagctagtag ccacatatga ggacggtagc cccgttcaat tttcaggaga tacatttatc
    12781 agttttaact ctttgaaccc tgcaggagga aaaaccacgg atcttaaagg agaatatgcc
    12841 cactatgaca aaatgaatac gacagattgg tatgtcagaa aagatacggt tctttcagaa
    12901 tttaaatctt tctacaacaa tctgaacgtg gtaggtggcc acccaggagg aagctctaag
    12961 ctgacacaag cggataatga tttcaataat ttgcatgata aattaggaga tccgaagttt
    13021 ggtcaaggta ctgtgtcctt taaaatatct gaagcaaatc cgacgtttgt gattggttct
    13081 tctaatgttc aaacctggtt tactttatct tctgcgacaa tctttagtgt tgttcctgat
    13141 caacctgaaa aaacaggggt tgataaaaat ggaaataacg tgaacgataa aatgttgcaa
    13201 gtaggagaca cgattcaata tcgaatcaaa cagaaggtga atcgattagg agtggattta
    13261 ctagcggttt acgatcgatt tgagttaatc gataatttac caaaagaagt aaattacgtc
    13321 gatgcacacg ttgaaagtgg tacaaataaa aaattcgatg tatctggtga agtgacctat
    13381 gacaaaacaa aacaccaggt aaaatatgca gcgaaagctg atacattaaa aaatcgaatg
    13441 aagtataacg gcgaaactta cgaattagtg atcaatgtaa aagtcaatga attggccaat
    13501 caaaatagtg tagccaaaaa tcagggaacg tcgatcatta ataaagtaga aaaggataca
    13561 aatatcatta ccgtttattt ccctaaaatt ccagtgaaag aagtacaaca aaatggaaaa
    13621 gatgtaaacg gacgaaatga cggtaagaag ggtacgccga cagctccttt aaacgcagga
    13681 tctgaagtac aatatctagt cacacaaaaa tggcacacaa aaggcgtaga cgctgcgtca
    13741 gatcactaca aacaatttag tattcaagat ccaattgaag ctcgtttgac atataaagaa
    13801 ggttctgcgc aagtaataga taagagtaaa ggaaaagata ttacttctga aggcacacta
    13861 acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgactt cttaagtaac
    13921 aatcttttag atggacgaga aattcagctg atatttacag ctaaaactcc attacaatca
    13981 gaaaaaaata ttgataacca agccgtagta gcagtagata atgtttctaa taaaacaaat
    14041 gttgtgacaa ttggggttga tcctaacttg ccacaggtca ttgttcctaa aacaggttct
    14101 acacatttag taacgatttc agcggtcagc ttactattac ttgtgttagc aacttttagt
    14161 tatgcggttc tgagatacat ttaaaataaa aaaacgaaag aatgaataaa atgaaaaagt
    14221 taataatagt agttacttcc ttgattgcca tgattgttat tttagtcgga tgtagtaatg
    14281 aaaaaaataa acaaactaat caagataatg gcgtattgaa atcaggaact atgtggaaag
    14341 aagaagtagg tggtcttgtt tataatttga aaattataga cgagactacc tgggaatact
    14401 ctgagagtgt atggcatcca gatcctgtac aaataacagt caagaggcaa aaagactata
    14461 aaggtttaga aagatacaaa atagtagatt cagctggtgt tagggaattt ataaacaaat
    14521 ctgattcttt atttattgtt gttccctatg agaagaatgg cgtaaaaaaa ataatttttc
    14581 ttggatctag caaggatgaa aagcgaacga aagagaaatt gattcacgat ggatcacaat
    14641 ccaataaata taagttacaa aagactagcg agtgattgct agtcttttta tttgtcctga
    14701 aggaggaatc acagtggcaa aagctaaaac gccatttgaa aaagccgcgt ctaaaatctt
    14761 agaacgccgc ggtgtatctg aaacacagtg gaaacaagaa gtccttgaaa aagcacaatt
    14821 tgacctatta cggggaaaag acaaggactt agaaggatat atctatcaac gtgaacgtga
    14881 aaagcttgtt ttcgacgaaa ttattaaaca ctaaaaggag cgaacaattt tatgtttgaa
    14941 tcaattacaa tgaatttttt aacacaaccg aaagtagtag ctgcggttga tatttttaga
    15001 gcattacaaa ctgccgcaac tagtttaaaa aacaatctga tgatttttgc tgctgctgcg
    15061 gccgttgctt ccattgttat tgggggtctt ttcttcctat ttggacgaag aggagctgaa
    15121 aatgggaaga tgcacgtagg ctatgtattg atgggggtag gagttatttt agcagcagct
    15181 tctattgtgg tttcgttatt tggattgttt ggacaaacac ctcaaggatt ataagaaagt
    15241 aaaaaagtag gagtaagaag tagtcgatac tttttactcc ttttttatga aaggagaata
    15301 ctcatgtact ttacgattgc aggcaatacc aaaacagata aagagttaat aaaaaaagca
    15361 acgaaggaag taggaaagcg tacttctttt ttaccgaaaa caattaaata ttctgtcatt
    15421 gaaacggaag aacagctaca tgtgttaggg aaagtactgg aacacgaagg tcgatcttct
    15481 gttaagttga ctctcacgtt acatgacaca gaaggaaaaa tagagaaagc tggtacagaa
    15541 tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tctattctac attgatggat
    15601 ttaatccgcc ttgatgtgtt taaagaaacg gatgaaacta tgagtgtgga agagtcttat
    15661 cgaaacgaaa aacttttaca agttttagaa gaagagttag agagcgacca aagaaaagtg
    15721 aatcaaggga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatatttcca
    15781 aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact
    15841 cagaaatcga tatttgagaa agagaacgat gtgatgggtc agttggaggt agatcccttt
    15901 gaagaaacaa tggaggatga agagacggcc gaagtagaag ttgatccagt tgaagctaat
    15961 gaagagtcaa catggactga aggggattct caggaatata ttgaaccact tatcagtgaa
    16021 gaagtagaaa aaaacgaaat agagatacaa aaggcaactg tatttgatta tattttggat
    16081 caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt
    16141 tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta
    16201 atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg
    16261 catgagattg cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat
    16321 caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagc aaaagaggaa
    16381 ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaaattaa agagcgatac
    16441 gaaaagaaac gcgacaatgc gtattccttg tgtgtagaaa agatcaataa ttttactcag
    16501 acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tataagagaa
    16561 cgaaaagaag aattttatca aggtttgcgt acagataagc taaagattag caaagaatta
    16621 aatgagcaac tgaataagct ctatcaaata acaagtgaag ctcttatgga aaaacacgag
    16681 cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa
    16741 caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgcgaacaa
    16801 ttagaactaa aaaaacgtga acaggatctt gcggaacaac aaatggcaat agaaaaacaa
    16861 cggttaaacc aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg
    16921 gatacattac gacaagctca gttaaattat ctagcacaac ctcaaccaac gccttcagta
    16981 gctgctgttc aaaccccaat gaacaaccaa acaaatctaa ctccttcacc acagcgttcc
    17041 tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg tggagggaca
    17101 gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtagc cacaacggcg
    17161 tcaatcaagg aagaagccca agaacaagca agaaaagagt atgaacaaca attggaagaa
    17221 ttattgaatc gtaccaatca ggcaacaaca gagtcaagta tggaaaatag caaagagagt
    17281 gatcctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa cacttccgaa
    17341 agttcagaat caaatgaggt gaaataatgc gtgttagaga aacaacgaga cgaaaagaca
    17401 aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat actccttcag
    17461 gagaagagct accagaaaaa catgacaaac gtatttctca aaagattttt tatagtggct
    17521 tagcttatct tcctttaaac tatgttgtta caagaatacc agaagcattt catcgatttg
    17581 agattattcc aggaagttta caaggagtga atatttttca gttacaagaa atgccgcagt
    17641 tattccattt tgattcaact ggtttttgga atcctttgac cccaacaaca agtttaggca
    17701 cacgtttagt tgtttctacg atttgtgttg ttccagctat acttatggga ttgaaaatgg
    17761 acatgtggtt tcgtcctcta gcagatgggc aaaaaggaga taaccgatta aaaacgttaa
    17821 aggaaatcca acaaatttat ccgcaaatac cagaatcaaa gggaggtttt cctggttatg
    17881 gcggaatacc aattactcat tataaaaagt actggtatat tcagacggat acagtaaata
    17941 cttgtattgt tggtacttcc cgttcaggga aaggacagat agaagtttta gcaaccattg
    18001 acaatttgtc tcgtgcagaa agacaaagtt caatggttgt taatgatcca aaaacagaat
    18061 tatatgttgc tagtaaagat gaactagaag ctagagggta tgatgtgtat gcatttaata
    18121 tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg aaatactgga
    18181 agcgaggaga catagatact gcaacgcagt taaccaatac ttttacgaat acgatttatt
    18241 atgatgccaa tgcttcagac aacaaatact ttaacgaaaa tgcgcagaaa gctgtaaatg
    18301 cgcttatttt tgcgttgtta gaacaagccg atcaaacagg ggattactcc aaattaacac
    18361 ccaataatct tgttgagctt ctttctgaaa ttggcggttt taattatcaa gatcctgaga
    18421 atgattacag acaactcaat gccttagatg aattcatgaa tcagttacca cctggaaaca
    18481 tagcaaaaaa acaatatggg gcaacaaaaa ttggttctga taaagcaaaa gggaatattt
    18541 tatcgacggc aatcacagga ttgaatccat ttaccctaac gaaaattgcg aaaatgactt
    18601 ctcaatcaac cattgattat aagtccattg gttttccgaa gtatttggat ctcaaactac
    18661 acgagagtct gttaaatcag cgaattgttg tagagtttta tcatcaaggg aaaaaatttc
    18721 atgaggaacg tgtcaaagta ggttatagag gtttttgtga aatcaacttt gattgtcatt
    18781 tagagcaggg agatcatgtt tgcttaaaat acgatcatca aggaaaagaa tatgttgctt
    18841 ggtatgagtt ttctcaaaga gtattgaaag acgaagaggg aaatatcgtt tatcgaaaaa
    18901 aacgaggaga aacccattta ccagaattag aaaaagaagc tgttctaaca ttagatgagg
    18961 ataaaagtaa cttattagta gagtctattc ggatgcacta taccgataaa ccaatggcta
    19021 tttttatgct cacacctgat tatgatccat ctaaccatat cattgttagt atttttctta
    19081 gtcagttgta taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc
    19141 gtaggattca ttttatttta gatgaatttg ggaatatgcc accaatggat aatatggaag
    19201 gaatcatgac agtcacagct ggtagaaaca tgttgtggga tctgtttatt caatcctatc
    19261 agcaacttta tgcgaagtat ggggaacaaa acgcagcgat tatcaaagaa aattgtcaga
    19321 cacatattta tattatgtcg acagacgata ataccatcga agaattttcc aaaaaagtgg
    19381 ggaataagac ggttgaacaa gagaacagta ccaccaattc attaggtatg aatactcata
    19441 tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct acttttttag
    19501 aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt cataaaatac
    19561 ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat caatatattg
    19621 aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca catgcggact
    19681 tggatcttaa ggctttacaa attgattacg attattttct acctgatatc tatgcacgtc
    19741 gactttatca caatatgcac gatcaaatag aagaggaagc taaagaaaac atttttatta
    19801 aaatcgttca atctttagac aactcaacgg cacaaaagtt acttattcaa ttttatgaag
    19861 agaagcaaat agatcgtttg caagaatacc ttgaaaaatt gcatcaaaat gaagaaatta
    19921 cagaggttat aaggaaacga cttttgctag aagctgaaaa ggcggtgaaa gaatgatcat
    19981 tggaggaata ttttccggca ttgccaactg gtggtcaggc ttatttgggg atggggatgc
    20041 agatcccaaa accgtaacga aagtcttaca agattatggt aattatctgc agtatcaaga
    20101 tgtattcagt tggatttgta atactttttt atggggtttg attaaagggt tatataatct
    20161 caatacttta ctagaaaaaa caatttatca aagctttgat ttaaaagact tattgaacgc
    20221 tgctggtgtt aatgaactgt ttcaaagttt gattagtaag gtattggctc ttttttgtgt
    20281 cgtcacatta atttatttag gcttaaaatt ttttatgagt aaacatccac caaaaatcaa
    20341 aaatatcttg gttaatctat ttatggccat gattttgatt tttggtggta gttctttaat
    20401 cgatgaaggg ctaaatatta gtaaaaactt ttacggagac gtgactgcag caaataaagc
    20461 agataaaact ggatcacctg cttttcaaat tattcaaaat aatgtgatcg atgtgagtac
    20521 gctactagaa actgatccga ataaagtaga atccctacca acagataaaa gaaatgctct
    20581 cacggcgaaa aactttaaga cagcaaatat taattcagtg attattccta aggaagccgc
    20641 taagatggcg gatgaagata agagcggtct atcagatata caaaaggatc ggctaagagc
    20701 gttacaatac cgactggaac tggatgataa tggaaaagaa atccctgtag agattagtga
    20761 tgaggggtta gcaaaatatg tgtatacttc aggctatcgc cgatacatta gtagtccagg
    20821 gattatttta gctggtgaat cttctttgtc ggttgcctat ttatttatct tgtttacgat
    20881 tgtaacttgc attattgagc tggtatttaa aaaattctat ttagtaattg ccgcgtcaac
    20941 ggactttgag acaggacaac ggatgaaaag tgctatagaa gatatttctc aatccttctt
    21001 gttattggcg tttacgattt tagaattacg aatctataca ttaatgctgt caggtatcgg
    21061 tgatttgcac gcagcaggca agatcaacgg atttttgtat gttgtcgctt taattgtact
    21121 aaccattgcg ctattcaaag gctctcaatc tgttacgaag attttcggtg ttgatacaag
    21181 tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa
    21241 tgtgggtagt ggggctaaaa accttgcaag tgctggtaag agtggtttaa gcaatttaaa
    21301 tgatattcgt aaaaatggat ttggcaatcg aaaagatgtt attcctgata acggggaaga
    21361 acaaccagac gataaagcta caattgtacc taatagtaag aaaacagttt ccgaaatgtt
    21421 cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg tcaatgaacg
    21481 tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct tagaagagaa
    21541 attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat ccgaagcact
    21601 agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg cgtatggtga
    21661 aggacaagtc aatgcagcag tgaaggcaaa tacaacaagt gaaaaagaaa atcaagaagg
    21721 tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg aaaattcgat
    21781 cggagaaaaa gattcagaag aaggacaaca tggacagtta ccaaaaaccg atccagaaca
    21841 atatcgttat aaatctaaca ttaattctga aaatgaaaag cgcatttcag atttaccaaa
    21901 acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ttgccaaatg ataatggaat
    21961 cgaagttgga actgaaaaat caatgaaaat tggtttgcct aagacacaaa atttaccaat
    22021 gaaagatagt cgtttgaatc aattcaagga gaatgatcaa agctttgttc accaatcaac
    22081 tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc tgacaactgt
    22141 ttcttacaat ttatctgaaa gtggtgagac agggaatgtg tccattcaaa atgttgtgac
    22201 aagagaagct gcaccagaac ctattcaaaa agttcacaca ggttacaaac cagcaaatgt
    22261 ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca ggaacaatga
    22321 attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa aggcctacga
    22381 gttaggcctt ttttctatgt atgaaaagga gaaatcattt atggataaat atattaacat
    22441 gaaaaatcct ccaagattta gtggggaatg gaagtttttc gcctttttct ttttagatct
    22501 attaacgctt ggtggttgct ttttttgggc gaaaatagtc aataatcttg tccacttacc
    22561 actctttttt gcactcttga attatggctt atggattgcg atagctctag tgattgtttg
    22621 gcgaccttat aataacccag gtcaacgaca aatttttgcg attatcgaat ctattttctt
    22681 tgcggataag aaccactatc atgcgttaga tcctaaccga aattatcatg gtacgtataa
    22741 ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gataagaaat ccattttgca
    22801 gtttggaaaa ccagaagaac gatctatctt ggatttattt ccttataaat cctgtacaga
    22861 agagggagtc ttagttacta aagaagatca ttttcaacgt tattatcgtg tagtttctac
    22921 agacgtggaa ggattaaacg aacaagagaa actagaacga atgaatcagt tgaccaccgt
    22981 catgcgtacg tatgtgcaaa caattaagtt agttagtttg accacagaga cggatctaac
    23041 ggaacaaatc gtacaaaagc ggcaattatt agataagaat cgtatggaac aatccttagg
    23101 aagaaatcta cagcgattaa gaaaatatga aaagaaatta gtagaggaaa tccaggaatt
    23161 gaaacgagca gaacaagaac gtcctgactt aagcttcttt ttcgtcattg aaggaaagac
    23221 aatcaaggat ttacaaacca gaaatcgtca gttaatgcga tctagtggca ttttagggtt
    23281 aaaaccactc tacaaaaaag aattgatcaa gattctttat cgaatgaaca atatgaatga
    23341 tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa aatgatgttc
    23401 cgaaggaaga gatcactaga aaattttctc acactttaag cctgggtaag ttgggaaaag
    23461 ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc
    23521 gattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat taaagcaact
    23581 aggaaatcgg gaaatagaaa ttttaaaatc caaagggtat gatttagatt ttttaaaaca
    23641 agtgatgcca caaggtggat taaatttcga tgaagagtat gtgacctatg gaaatggtta
    23701 ttcacgatgt gtgacagtct atcactatac aaaaaatcct actctttttt ggttggccaa
    23761 tctcatgaac aatcctcata cagtggccac actagatgtt agcacagatg aaaagcaaaa
    23821 agtgattgat caaatcaacc gcgcattaga tgaattaaat gatcgatcag ataatgaacg
    23881 aaaagcgact gaccgaaata aaagtgcgga agaatacctg gaactagcgg aatatgcccg
    23941 aaaattgact tctaatgggg aaatttctaa acaagtcaaa gtgagagtac atgtttacgg
    24001 aaatacgata caagaagtgg atgaacgggc gcaggaactg atcgaaaatc taaaagggat
    24061 ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac
    24121 catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac taccatcgtt
    24181 aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag gaggtacaca
    24241 atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata aaaatagtgt
    24301 acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct
    24361 taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg
    24421 aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc
    24481 tgacgggatg atcaatcctt tagagatatt tgccacacgg acgtatgatg aagcatccag
    24541 gttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac
    24601 gcacttagac aaagtagtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat
    24661 gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa cagtaccacg
    24721 tggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag gtaaagatcc
    24781 agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg
    24841 tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg
    24901 tgagacgtat ggcatgattt ttgatggtca cagtacgatt cgacaccttg accaacaaca
    24961 gttggtcttt tttgacattg actctattac aggtttagat cgaggggtgc aacaatgtca
    25021 aatgtacatg gctatgacgt tgatttggaa tcaggcattg atccagggaa gaagacaacg
    25081 acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat
    25141 tgatgagtgc cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt
    25201 ctaaaaacta ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa
    25261 gggcttccac tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa
    25321 gctcggtgaa gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag
    25381 ttctttagag gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg
    25441 agtatgtaca aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag
    25501 aaatgggaga gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag
    25561 tcgttgttat gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct
    25621 agcatctaag ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga
    25681 gagtgcatac tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag
    25741 tgatggtgaa agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc
    25801 tcgattcgtc tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa
    25861 aggtcacgag ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag
    25921 gaagttgcgg catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa
    25981 aagtttgaag ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat
    26041 tgtcctagca tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg
    26101 agtaacgatt aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa
    26161 gcgattcgca cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg
    26221 taaattaaga ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt
    26281 acaagtatta gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc
    26341 aaatagatca gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat
    26401 ggaatatgtg gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact
    26461 catgagacaa atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa
    26521 aatgttaaga gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac
    26581 accacaaggc ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg
    26641 gtggatagca aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa
    26701 aaatggagct tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa
    26761 agaaatatac ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga
    26821 agcgaaaaaa gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat
    26881 atctgaagaa aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt
    26941 tacgatgaaa ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa
    27001 agcacaaagc atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc
    27061 taatagcaaa gataccttta tctatattgg agtctataac agtatggtta ttgggattca
    27121 taattacttc aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact
    27181 cagaccaagt tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg
    27241 aaagtacgaa ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt
    27301 tccaatatta ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat
    27361 taataaatac accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga
    27421 atggaagata tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt
    27481 tgcggacaat cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga
    27541 ggagcttatc ttatctgaaa tgcattgtca tcatattatc ccttatcatg aaagcaaaag
    27601 tgatagctat gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac
    27661 acaaagtgaa acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga
    27721 aaaactcaat gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt
    27781 aggacgggaa aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc
    27841 acgtacggtg tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat
    27901 cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc
    27961 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
    28021 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
    28081 tctttatgcg tatggatgaa tctattttac gtcatataga aggactagtg ggtaaatcaa
    28141 tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaact
    28201 ttggatcaaa agaaaccatt cgcgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
    28261 ttgatggagg aaaataatgg aaaatcaaga tgaaagttta atcaagcagt atgttaagcg
    28321 tcgcgcaaag cggcgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat
    28381 tttgatcact gtgtttgtta ccctatttct aatacttgcg gcgggttcga ttgataattc
    28441 ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
    28501 gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgccgt
    28561 aggtgcagca gtgaaatata aattattacc aagtgtgatt ctttcgcaat acggttatga
    28621 atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aactattttg ggattacttg
    28681 gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg
    28741 ctggtacatg aagtttccca actcaaaagc tgcattctcc tattacggct ttatggtagc
    28801 cacacaatca aattttaatg catgtgtagg aaataaaagc cctggcgcaa gcttattgat
    28861 cttaggacga ggtggttatg ccgctgcagg aattactgaa gatagtccgt attatacagg
    28921 ttgtatgtcg attattacct caaataaatt aacagaatat gatgaatttg cgatcaaaca
    28981 ttggggagaa ggcggaaatg acaacggtac gattactggt gaatggacga atcctttccc
    29041 tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg
    29101 cggtgaattc cgtccgaatg gtttccatga tggattagac tttggttcag ttgatcatcc
    29161 aggaagtgag attcatgcgg ttcatggagg aaaagtcgtg tacgttggaa atcctggtat
    29221 ttcaggtttg ggcgcgtgtg tgatcgtgat taattacgac ggcttgaata tggtatatca
    29281 agaatttgca aatagtacag gaaattcccg tgtgaaagtg ggagatcaag tgaaagttgg
    29341 tcaagtaatc gggatacgag atacagcaca cttgcattta ggcttcactc gaatggattg
    29401 gcgtcaagca caaggacacg cattcacaga tgatggtaca tggatcgatc cattaccatt
    29461 tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat
    29521 cgtcttaaca gctgcactca gcattagctt aattggcaat atcgcgctct tttggcagct
    29581 gaaagaggaa caagcgacaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
    29641 gaagctcaaa aaaaatttct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat
    29701 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gacggtataa aagcaaaaga
    29761 agaagtcaca gatgcccaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
    29821 atcactaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
    29881 cgacaaatta agtcctgcag atgatgatca ccaacatgat gattatggaa gttactcctt
    29941 tacattaggt gacgtgcaaa cttacgctga aacaagcagt gtaaaagagg aacaaaattt
    30001 tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca aaccgcaaaa
    30061 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcacaga
    30121 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac
    30181 gttttaaaaa acggaaaagt tatcttagat ggctgggaat ctttacgatt ctcggctttt
    30241 tgattcttgc tagtccgatt tatacgcata agaaggtaaa cataccagat gaggtaagca
    30301 cgaacgcttt tttaaacacg aaagaactga caatggtatc aaaagaaaaa ggagcgaatc
    30361 agcaattagt ttttgaattt gcgattgacg atgaggatca aggcttatta aaagaactgg
    30421 ctaatcttga ttatcgcgta gaagtaaaga cagctaaagg tgattatcaa gcaataaaag
    30481 ccaaagtgat caaggtgtct gatgattatt ttgtagtgaa attagcacat gtaccagaag
    30541 aatatatcgc catgcgttta accattgttc ctgaaaaaat tgatccaaaa gtggatatgc
    30601 aagaaccaca agatctgatt tattacgttc acgaagataa ggttaaggat caagtgaaag
    30661 atgataatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
    30721 aagcagcaca aaaacagatt gaatccttgc aagcaaccat tgatttgaat gaagaattag
    30781 tgaataaact aaaagaccaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa
    30841 ataagattaa tgattaccag caagaaattg aaacatcgaa aacgcaaatg aaagatcaag
    30901 aagagatcat tcaaaaaatt caagaaaaaa ttgatcgtat gacaaaagaa aatatttagt
    30961 agaacaggtg gagtgaaaga aatgaaggga gcgaacttgg tcggcaaaga tattttcgct
    31021 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcaa
    31081 gaagtgaatt tatctaacac aacaatatct gagttgaaga atggtagaaa gagtttagat
    31141 accttaaaaa tcagatcatt tgaagctttg tatttagctg ctaaaaaata ttttacggct
    31201 gaaagaaaat aaaaacagga ggattttatg tgacgcgaat tgtaaaatta agttagaaaa
    31261 ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa caaaggagtg
    31321 taaccataaa tgacctacaa acatcttacc atagacgaac tgacaatgat agaatcatat
    31381 tatcttcaac ataataagcc ggttgaaatc gctaaccgaa tgggacgtgc tatacaaact
    31441 atttataatg tagtcaataa gttcaagcaa ggcaagactg ctcttgatta ttggcaccag
    31501 tataaagaaa ataagaaaaa atgtggtaga aaagtcattc aattacctgc tcatgaagta
    31561 gattacatta aagagaaagt cactcttggt tggacgcctg acgtcattat cgggcgaaaa
    31621 gaaaggcttg tttcatgcgg tatgagaaca ctttatcgtt tattttctaa aggaatattt
    31681 gagattgaca cactaccgat gaaaggtaaa agaaaaccca atggccatca ggaaaaacgg
    31741 ggaaaacaac agtatcagcg ctcaatccat gatcgacctg ataattatcc tgatttcaat
    31801 tctgagtttg gtcaccttga aggtgatacg atcgttggca ttcatcataa aagtgccgtc
    31861 attactttag ttgaaagatt atctaaagtc attatcacga ttaaacccaa cggccgtaag
    31921 gcattagata ttgaaactgc ccttaatcaa tggttttctc gcttccctaa aaacttcttt
    31981 aaatctatta cgtttgactg tggaaaagaa ttttctaact ggaaagccat tagtaaccaa
    32041 catgatattg atatatattt tgcggaccct ggaacacctt ctcaatgccc attaaacgag
    32101 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagagaagtg
    32161 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
    32221 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
    32281 ttaatttgac aaatcatatg aaaaaagtat ataaaaaatg tttatggatc agctatatca
    32341 ccctaggtat agctgttaaa gagatgggag aaaacattaa ccacgattct aacacaaaca
    32401 tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa ccaaacagga
    32461 agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat atgacaaaat
    32521 atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca caaataacct
    32581 taatttttaa agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg
    32641 ctgtgcctat tacgtttctc taaaacaaaa gtgggtacaa aatattcgat ttatggatag
    32701 tgtatggata gtaaaacagc tatacatgga tagcatatgg atagactcca tagactatcc
    32761 atactttttt cggagggtga aaatggaaat taaagagatt aaagtaagag gtattaataa
    32821 aaaatatgtt caagaaattg atcatagatg cgtagaatta acagaacaaa ctggtcaaaa
    32881 atggagtcga aatgattatc taaaactatt gattgaaaac gactttgaac gtcctttaat
    32941 ggactacaag aaagatcaat ttgatcgttt attggaaaaa tttacagatg ttcagctgca
    33001 taatacaaaa gttttggaag catacacgaa cgaggtcaag aacttaattg aaatattaat
    33061 cgcacattag gaaggagata atgttctatg gttaaagcga tagaaaagca tattacattt
    33121 tatgaagcgc acgatgaagt tttaaaacat attatggaaa ctcagcctac ggtaaaaaac
    33181 tattctgaag ccattcgatt tttatgtatg aattacgatc cagaaattga aaaaaataat
    33241 aatatgacga acgcaaagtt gaacgcaatg ggaaaggaaa tttctatttt aatagaaatg
    33301 gtttcagagt ttgcggatct tcatttgaaa gatacaggaa tattaattgg tacaaattca
    33361 actgtctatc aagaagcaaa agagcgtgtg gaagggaaaa taaaagcgaa tcaaacgaag
    33421 aagtattctc ctaaaatcaa gaaatcaata aatgaatctg ttcatgccag agtagacccg
    33481 tttgatccaa caaagttttt aaaaagataa aaagtagaag gggaatatga tgaataaatc
    33541 gcctgcagtc attctcactt gtcaatttac cttgcccaat aaaaaatctt tttcaacata
    33601 cattgattat atgacaagag aaaaagcgct ggaagaaatg gaacgacgta cgcctgaaga
    33661 agaacaggaa ctgcaacgaa tcaagaatgc tttagaggat ttttatattc ctgaaggaga
    33721 aactttttcg gagagtaaga cggataaaga attcagcgaa acgtcgaatg aagctcaatc
    33781 tttgatggga aatggaatca actttgacaa gttagaggaa caagatttta ccaaatatct
    33841 ttcttatatg acccgtcact atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca
    33901 aaaagaatac caacgcttga accaagcaat aaaaaaatat caaacagagc cacacaaaaa
    33961 agcttcagga aaccttcctg gagttttttc tatggcttca gacgaagtga aaggaagtga
    34021 tctgaaggag attaagcaaa tcttccaaag aggacaacaa aaaggctcaa tcatttatca
    34081 agatgttgtc tctcatgata attcgtatct tgaaaaaata ggattgtacg atcccaaaac
    34141 agatacttta gatgaagaag gattaaaagc agctggaaga aaaatgatgg agacattatt
    34201 cgagaaagag caactgaacg aaacaggcta ctggatggca acgattcacc gtaataccaa
    34261 acatatccat attcattttg cagttgttga aaaagaaaat acgcgtaaac cttacacgga
    34321 ggttgaaaat ggtgtgagtt atatcgtacc gaaaggtaag agaagacaag caacattaga
    34381 cgcaatgaag accaattatg tacatgagtt agaacgtttt tcttatgaaa aggatcgaat
    34441 gattggtatg gagaaacgaa atttattgac gcggaaaagt gatttgcgta acaccttaac
    34501 aaaagtggta aaagagccag cgcgttacga tcgacacgca atgaacttat taaatgaagt
    34561 atataaaacc ctacctgaca aacgttcgga atggcattat ggggatgaaa aacgaaccaa
    34621 actttcacca tacacaagag agaaactcga tgagttaacc aaatatgttt taacaaaaga
    34681 acctgaatat caagaatata tacagatttc aaaagcattg aaagaagaag caaaaagtct
    34741 ttatggggaa agcaaaagag aatcaaagga tgcagaaaaa aatgctctct ttgatttgaa
    34801 aaagcgcaca ggaaatgcca ttctaacaga actgaagaaa cagaatagtc aaataaaacc
    34861 tttaatagaa agtatggatc agatgcaatc tatttacaat gaacgatcct ttcgaacgct
    34921 taaacagtta ccaacgaatg aatacaaata tagttacgaa gaattcatga agcgcaggaa
    34981 acaattccaa caaagagttg tcacgcaacg gagtttgcgt caattaaatc gaacgattaa
    35041 tgcccatgaa gaaaaatacc acgcaagtaa agcctacgaa gagcaacaac gaagaatcca
    35101 aatagaacaa gaaagacagt aaagacagta aagttagcgc cttattatac tacttgggaa
    35161 atgaatgaag aggggatcaa taacttcccg tgtaaaagca ttgaagagtc tatttcactc
    35221 acagaatgtt agttgttaag tgaataaaag aagtaaaaga gtcctgaacg taattgtcca
    35281 ggactctttt tttcaatgaa ttgttttccg ccaattcaat gagtggtgcg atttgcgact
    35341 acattatatc aaaagaaaga gaagggagga agaagaaatg gcttattcaa cgattggagt
    35401 tgttgttgaa aaaagcagag acaatctcgt ttttgtgaca gaaattcaaa caggcagagc
    35461 ttttgtcgtc acagacaaag cggcaaaagc gtatcaaaat ggcgatattt taacgttaaa
    35521 tctgactacg aagacgttcg tcgatgcggc agaagattat ccatt
//
LOCUS       DACIFS010000028        35388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.028, whole genome shotgun sequence.
ACCESSION   DACIFS010000028 DACIFS010000000
VERSION     DACIFS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.028"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(3..1529)
                     /locus_tag="IYA83_001360"
     CDS             complement(3..1529)
                     /locus_tag="IYA83_001360"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ8586801.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1544..2191)
                     /locus_tag="IYA83_001361"
     CDS             complement(1544..2191)
                     /locus_tag="IYA83_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ8586802.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2375..2704)
                     /locus_tag="IYA83_001362"
     CDS             complement(2375..2704)
                     /locus_tag="IYA83_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ8586803.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2881..3609
                     /gene="sfsA"
                     /locus_tag="IYA83_001363"
     CDS             2881..3609
                     /gene="sfsA"
                     /locus_tag="IYA83_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ8586804.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3625..4638
                     /locus_tag="IYA83_001364"
     CDS             3625..4638
                     /locus_tag="IYA83_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8586805.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4638..5915
                     /locus_tag="IYA83_001365"
     CDS             4638..5915
                     /locus_tag="IYA83_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ8586806.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            5978..8680
                     /locus_tag="IYA83_001366"
     CDS             5978..8680
                     /locus_tag="IYA83_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8586807.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8832..9149
                     /locus_tag="IYA83_001367"
     CDS             8832..9149
                     /locus_tag="IYA83_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8586808.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9179..9499
                     /locus_tag="IYA83_001368"
     CDS             9179..9499
                     /locus_tag="IYA83_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ8586809.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9607..11067
                     /locus_tag="IYA83_001369"
     CDS             9607..11067
                     /locus_tag="IYA83_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8586810.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11135..11356
                     /locus_tag="IYA83_001370"
     CDS             11135..11356
                     /locus_tag="IYA83_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586811.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11387..11569
                     /locus_tag="IYA83_001371"
     CDS             11387..11569
                     /locus_tag="IYA83_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ8586812.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11569..11982
                     /locus_tag="IYA83_001372"
     CDS             11569..11982
                     /locus_tag="IYA83_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ8586813.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12105..13286
                     /locus_tag="IYA83_001373"
     CDS             12105..13286
                     /locus_tag="IYA83_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8586814.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            13675..13748
                     /locus_tag="IYA83_001374"
     tRNA            13675..13748
                     /locus_tag="IYA83_001374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13709..13711,aa:Arg,seq:cct)
     gene            complement(13785..13985)
                     /locus_tag="IYA83_001375"
     CDS             complement(13785..13985)
                     /locus_tag="IYA83_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ8586815.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(14290..15522)
                     /locus_tag="IYA83_001376"
     CDS             complement(14290..15522)
                     /locus_tag="IYA83_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ8586816.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            15776..16345
                     /locus_tag="IYA83_001377"
     CDS             15776..16345
                     /locus_tag="IYA83_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8586817.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(16523..16963)
                     /locus_tag="IYA83_001378"
     CDS             complement(16523..16963)
                     /locus_tag="IYA83_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ8586818.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            17121..17885
                     /gene="map"
                     /locus_tag="IYA83_001379"
     CDS             17121..17885
                     /gene="map"
                     /locus_tag="IYA83_001379"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ8586819.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            17917..18840
                     /locus_tag="IYA83_001380"
     CDS             17917..18840
                     /locus_tag="IYA83_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ8586820.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            18916..20055
                     /locus_tag="IYA83_001381"
     CDS             18916..20055
                     /locus_tag="IYA83_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ8586821.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20048..20848
                     /locus_tag="IYA83_001382"
     CDS             20048..20848
                     /locus_tag="IYA83_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8586822.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            20848..21675
                     /locus_tag="IYA83_001383"
     CDS             20848..21675
                     /locus_tag="IYA83_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8586823.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            21653..22387
                     /locus_tag="IYA83_001384"
     CDS             21653..22387
                     /locus_tag="IYA83_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ8586824.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            22487..23353
                     /gene="rfbA"
                     /locus_tag="IYA83_001385"
     CDS             22487..23353
                     /gene="rfbA"
                     /locus_tag="IYA83_001385"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ8586825.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            23367..23939
                     /gene="rfbC"
                     /locus_tag="IYA83_001386"
     CDS             23367..23939
                     /gene="rfbC"
                     /locus_tag="IYA83_001386"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ8586826.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            23961..24989
                     /gene="rfbB"
                     /locus_tag="IYA83_001387"
     CDS             23961..24989
                     /gene="rfbB"
                     /locus_tag="IYA83_001387"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ8586827.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            25087..25938
                     /gene="rfbD"
                     /locus_tag="IYA83_001388"
     CDS             25087..25938
                     /gene="rfbD"
                     /locus_tag="IYA83_001388"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ8586828.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            25973..28006
                     /locus_tag="IYA83_001389"
     CDS             25973..28006
                     /locus_tag="IYA83_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586829.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            28050..29330
                     /locus_tag="IYA83_001390"
     CDS             28050..29330
                     /locus_tag="IYA83_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ8586830.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            29540..30346
                     /locus_tag="IYA83_001391"
     CDS             29540..30346
                     /locus_tag="IYA83_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586831.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            30358..31578
                     /locus_tag="IYA83_001392"
     CDS             30358..31578
                     /locus_tag="IYA83_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586832.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            31568..33154
                     /locus_tag="IYA83_001393"
     CDS             31568..33154
                     /locus_tag="IYA83_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8586833.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            33193..35331
                     /locus_tag="IYA83_001394"
     CDS             33193..35331
                     /locus_tag="IYA83_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ8586834.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT        11212 a         6067 c         7356 g        10753 t
ORIGIN      
        1 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
       61 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
      121 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
      181 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
      241 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
      301 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
      361 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
      421 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
      481 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
      541 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
      601 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
      661 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
      721 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
      781 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
      841 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
      901 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
      961 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
     1021 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
     1081 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
     1141 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
     1201 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
     1261 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
     1321 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
     1381 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
     1441 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
     1501 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
     1561 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
     1621 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
     1681 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
     1741 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
     1801 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
     1861 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
     1921 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
     1981 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
     2041 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
     2101 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
     2161 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
     2221 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
     2281 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
     2341 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
     2401 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
     2461 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
     2521 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
     2581 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
     2641 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
     2701 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
     2761 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
     2821 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
     2881 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
     2941 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
     3001 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
     3061 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
     3121 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
     3181 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
     3241 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
     3301 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
     3361 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
     3421 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
     3481 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
     3541 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
     3601 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
     3661 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
     3721 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
     3781 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
     3841 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
     3901 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
     3961 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
     4021 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
     4081 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
     4141 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
     4201 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
     4261 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
     4321 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
     4381 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
     4441 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
     4501 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
     4561 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
     4621 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
     4681 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
     4741 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
     4801 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
     4861 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
     4921 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
     4981 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
     5041 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
     5101 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
     5161 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
     5221 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
     5281 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
     5341 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
     5401 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
     5461 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
     5521 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
     5581 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
     5641 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
     5701 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
     5761 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
     5821 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
     5881 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
     5941 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
     6001 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
     6061 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
     6121 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
     6181 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
     6241 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
     6301 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
     6361 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
     6421 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
     6481 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
     6541 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
     6601 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
     6661 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
     6721 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
     6781 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
     6841 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
     6901 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
     6961 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
     7021 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
     7081 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
     7141 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
     7201 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
     7261 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
     7321 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
     7381 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
     7441 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
     7501 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
     7561 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
     7621 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
     7681 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
     7741 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
     7801 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
     7861 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
     7921 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
     7981 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
     8041 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
     8101 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
     8161 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
     8221 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
     8281 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
     8341 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
     8401 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
     8461 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
     8521 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
     8581 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
     8641 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
     8701 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
     8761 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
     8821 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
     8881 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
     8941 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
     9001 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
     9061 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
     9121 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
     9181 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
     9241 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
     9301 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
     9361 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
     9421 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
     9481 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
     9541 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
     9601 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
     9661 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
     9721 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
     9781 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
     9841 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
     9901 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
     9961 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    10021 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    10081 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    10141 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    10201 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    10261 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    10321 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    10381 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    10441 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    10501 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    10561 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    10621 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    10681 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    10741 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    10801 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    10861 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    10921 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    10981 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    11041 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    11101 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    11161 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    11221 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    11281 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    11341 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    11401 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    11461 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    11521 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    11581 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    11641 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    11701 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    11761 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    11821 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    11881 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    11941 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    12001 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    12061 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    12121 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    12181 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    12241 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    12301 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    12361 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    12421 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    12481 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    12541 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    12601 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    12661 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    12721 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    12781 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    12841 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    12901 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    12961 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    13021 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    13081 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    13141 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    13201 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    13261 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    13321 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    13381 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    13441 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    13501 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    13561 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    13621 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    13681 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    13741 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
    13801 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
    13861 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
    13921 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
    13981 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
    14041 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
    14101 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
    14161 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
    14221 tctaagccaa tagcttagat cctttattct atgctttata ttattttttg catgtgtgcg
    14281 aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc catcttcacg
    14341 gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg tttgacttct
    14401 attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt tcacactgaa
    14461 ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa
    14521 aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga cttctcctaa
    14581 acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc cttgttcggc
    14641 agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga tgatggtgcc
    14701 agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac tgtcaggtgc
    14761 tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag cattatagct
    14821 tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg taccgggagc
    14881 agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt ttgctaattt
    14941 ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag tcgttttgaa
    15001 aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga agacttttgc
    15061 tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg cttgagttgc
    15121 cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta ctcgatggct
    15181 atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc ttgctccttt
    15241 ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa tacgatccgt
    15301 tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa cgataacctc
    15361 agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg cctgttccac
    15421 gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt ctgcaattaa
    15481 gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa ttcctcctca
    15541 ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa caatgaggct
    15601 ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat gttactactt
    15661 gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac
    15721 aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc
    15781 aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac
    15841 cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca
    15901 accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat
    15961 ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga
    16021 tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta
    16081 cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa
    16141 tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta
    16201 taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca
    16261 tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt
    16321 caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca
    16381 agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc
    16441 cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga
    16501 tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta
    16561 aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg
    16621 atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt
    16681 aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca
    16741 agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag
    16801 ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga
    16861 tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag
    16921 tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt
    16981 ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa
    17041 agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt
    17101 tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg
    17161 cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag
    17221 gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa
    17281 ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg
    17341 aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg
    17401 atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag
    17461 aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta
    17521 ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg
    17581 tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga
    17641 ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag
    17701 aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg
    17761 atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata
    17821 cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt
    17881 attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa
    17941 caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa
    18001 atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt
    18061 attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat
    18121 ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt
    18181 ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca
    18241 agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga
    18301 aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct
    18361 attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca
    18421 atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca
    18481 ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg
    18541 aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca
    18601 caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata
    18661 ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc
    18721 ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga
    18781 gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag
    18841 aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg actcttaatt
    18901 aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt
    18961 ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc
    19021 gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct
    19081 atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg
    19141 tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat
    19201 aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt
    19261 tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa
    19321 ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc
    19381 aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg
    19441 ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt
    19501 ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt
    19561 tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa
    19621 gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg
    19681 gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca
    19741 gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct
    19801 gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat
    19861 actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta
    19921 tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt
    19981 ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt
    20041 catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag
    20101 tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt
    20161 aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta
    20221 taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg
    20281 acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat
    20341 tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg
    20401 tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga
    20461 gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa
    20521 gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta
    20581 tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta aagagataaa
    20641 ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga
    20701 aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa
    20761 gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt
    20821 caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt
    20881 tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa
    20941 aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt
    21001 gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat
    21061 aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat
    21121 acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt
    21181 gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat
    21241 cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc
    21301 atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt
    21361 aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg
    21421 gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat
    21481 gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat
    21541 gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa
    21601 cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag
    21661 aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata
    21721 tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat
    21781 ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga
    21841 taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc
    21901 gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga
    21961 gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt
    22021 ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt
    22081 tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg
    22141 tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat
    22201 gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt
    22261 aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg
    22321 gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa
    22381 acgttgataa aagatgttta aatccttaat atttccatga caaatataac aattgatgag
    22441 ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat
    22501 tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt
    22561 gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg
    22621 aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt
    22681 tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg
    22741 attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt
    22801 attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc
    22861 aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt
    22921 agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa
    22981 atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa
    23041 aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt
    23101 ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg
    23161 aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa
    23221 cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca
    23281 attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt
    23341 ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca
    23401 aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa
    23461 aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat
    23521 cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa
    23581 aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta
    23641 gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc
    23701 ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt
    23761 ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt gatgatccag
    23821 atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc
    23881 gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag
    23941 aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg
    24001 gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg
    24061 ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag
    24121 aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg
    24181 acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc
    24241 catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg
    24301 atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag
    24361 acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc
    24421 cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc
    24481 gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc
    24541 atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat
    24601 tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag
    24661 tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg
    24721 aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg
    24781 catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa
    24841 aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg
    24901 acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg
    24961 aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa
    25021 aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat
    25081 aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta
    25141 ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat
    25201 gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca
    25261 gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat
    25321 gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat
    25381 atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac
    25441 caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa
    25501 gagattttag aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac
    25561 aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat
    25621 gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc
    25681 gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat
    25741 gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa
    25801 gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa
    25861 gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc
    25921 aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa
    25981 acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt
    26041 ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat
    26101 cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag
    26161 agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga
    26221 atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa
    26281 cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga
    26341 tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg
    26401 actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact
    26461 gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt
    26521 ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt
    26581 gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac
    26641 gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc
    26701 ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta
    26761 tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt
    26821 tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt
    26881 gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt
    26941 tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc
    27001 tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt
    27061 atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct
    27121 gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct
    27181 gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc
    27241 gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat
    27301 tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa
    27361 ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt
    27421 tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc
    27481 tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc
    27541 aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga
    27601 attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc
    27661 agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg
    27721 gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc
    27781 agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt
    27841 gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct
    27901 atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga
    27961 tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga
    28021 ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat
    28081 ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt
    28141 ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta
    28201 tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt
    28261 ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa
    28321 gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa
    28381 ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct
    28441 tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca
    28501 tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt
    28561 ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg
    28621 ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat
    28681 ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt
    28741 tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc
    28801 cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata
    28861 tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga
    28921 aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta
    28981 ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta
    29041 ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct
    29101 tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc
    29161 cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg
    29221 aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg
    29281 aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa
    29341 atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca
    29401 tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat
    29461 aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa
    29521 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca
    29581 gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
    29641 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
    29701 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
    29761 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
    29821 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
    29881 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
    29941 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
    30001 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
    30061 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
    30121 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
    30181 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
    30241 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
    30301 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
    30361 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
    30421 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
    30481 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
    30541 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
    30601 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
    30661 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
    30721 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt
    30781 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
    30841 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
    30901 tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta
    30961 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
    31021 aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
    31081 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
    31141 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg
    31201 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
    31261 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
    31321 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
    31381 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
    31441 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
    31501 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
    31561 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
    31621 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt
    31681 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga
    31741 attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt
    31801 tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt
    31861 agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga
    31921 aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt
    31981 caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta
    32041 tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga
    32101 ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga
    32161 agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc
    32221 attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt
    32281 gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt
    32341 caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg
    32401 aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg
    32461 agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
    32521 gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac
    32581 tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga
    32641 aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga
    32701 aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac
    32761 cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg
    32821 gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga
    32881 cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa
    32941 ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc
    33001 aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc
    33061 aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc
    33121 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
    33181 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
    33241 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
    33301 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
    33361 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta
    33421 tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc
    33481 atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa
    33541 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
    33601 gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
    33661 agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac
    33721 cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
    33781 tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat
    33841 gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga
    33901 attaaagtcg ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg
    33961 gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt
    34021 gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt
    34081 gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg
    34141 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcggacaagc
    34201 attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg
    34261 gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg
    34321 tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc
    34381 tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc
    34441 gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac
    34501 ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat
    34561 tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
    34621 acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt
    34681 atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
    34741 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
    34801 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
    34861 ttattttatc cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt
    34921 gcaggacatg cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc
    34981 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
    35041 aatgctgtca atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc
    35101 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat
    35161 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg
    35221 gaaatcaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
    35281 gataatctaa ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa
    35341 ctgtattttc atttttatta atgaaaatac agttctttta atttaatt
//
LOCUS       DACIFS010000029        34662 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.029, whole genome shotgun sequence.
ACCESSION   DACIFS010000029 DACIFS010000000
VERSION     DACIFS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.029"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(313..2205)
                     /locus_tag="IYA83_001395"
     CDS             complement(313..2205)
                     /locus_tag="IYA83_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586835.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IYA83_001396"
     CDS             complement(2202..3929)
                     /locus_tag="IYA83_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586836.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IYA83_001397"
     CDS             complement(3945..4595)
                     /locus_tag="IYA83_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8586837.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IYA83_001398"
     CDS             4799..5095
                     /locus_tag="IYA83_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ8586838.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IYA83_001399"
     CDS             5287..5907
                     /locus_tag="IYA83_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ8586839.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IYA83_001400"
     CDS             complement(5999..7165)
                     /locus_tag="IYA83_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ8586840.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IYA83_001401"
     CDS             complement(7177..7530)
                     /locus_tag="IYA83_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ8586841.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IYA83_001402"
     CDS             7658..8680
                     /locus_tag="IYA83_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ8586842.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IYA83_001403"
     CDS             complement(9119..9553)
                     /locus_tag="IYA83_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586843.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IYA83_001404"
     CDS             complement(9663..11522)
                     /locus_tag="IYA83_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ8586844.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12800)
                     /locus_tag="IYA83_001405"
     CDS             complement(11640..12800)
                     /locus_tag="IYA83_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ8586845.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IYA83_001406"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="IYA83_001406"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ8586846.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="IYA83_001407"
     CDS             complement(14547..15245)
                     /locus_tag="IYA83_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ8586847.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="IYA83_001408"
     CDS             complement(15202..16863)
                     /locus_tag="IYA83_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ8586848.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="IYA83_001409"
     CDS             17081..18481
                     /locus_tag="IYA83_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ8586849.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="IYA83_001410"
     CDS             complement(18733..19812)
                     /locus_tag="IYA83_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8586850.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="IYA83_001411"
     CDS             complement(19898..20857)
                     /locus_tag="IYA83_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ8586851.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22392)
                     /locus_tag="IYA83_001412"
     CDS             complement(20869..22392)
                     /locus_tag="IYA83_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ8586852.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22965)
                     /locus_tag="IYA83_001413"
     CDS             complement(22402..22965)
                     /locus_tag="IYA83_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ8586853.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22971..23834)
                     /locus_tag="IYA83_001414"
     CDS             complement(22971..23834)
                     /locus_tag="IYA83_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8586854.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23848..24744)
                     /locus_tag="IYA83_001415"
     CDS             complement(23848..24744)
                     /locus_tag="IYA83_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8586855.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24919..26190)
                     /locus_tag="IYA83_001416"
     CDS             complement(24919..26190)
                     /locus_tag="IYA83_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8586856.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26180..27697)
                     /locus_tag="IYA83_001417"
     CDS             complement(26180..27697)
                     /locus_tag="IYA83_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ8586857.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27895..28785
                     /locus_tag="IYA83_001418"
     CDS             27895..28785
                     /locus_tag="IYA83_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ8586858.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IYA83_001419"
     CDS             complement(28858..29682)
                     /gene="nadE"
                     /locus_tag="IYA83_001419"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ8586859.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29694..31166)
                     /locus_tag="IYA83_001420"
     CDS             complement(29694..31166)
                     /locus_tag="IYA83_001420"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ8586860.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31803..32165)
                     /locus_tag="IYA83_001421"
     CDS             complement(31803..32165)
                     /locus_tag="IYA83_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ8586861.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32388..33140
                     /locus_tag="IYA83_001422"
     CDS             32388..33140
                     /locus_tag="IYA83_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8586862.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33280..34203)
                     /locus_tag="IYA83_001423"
     CDS             complement(33280..34203)
                     /locus_tag="IYA83_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ8586863.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34218..34490)
                     /locus_tag="IYA83_001424"
     CDS             complement(34218..34490)
                     /locus_tag="IYA83_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586864.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7213 c         5980 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
    22861 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
    22921 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
    22981 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
    23041 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
    23101 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
    23161 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
    23221 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
    23281 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
    23341 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
    23401 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
    23461 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
    23521 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
    23581 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
    23641 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
    23701 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
    23761 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
    23821 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
    23881 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
    23941 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
    24001 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
    24061 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
    24121 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
    24181 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
    24241 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
    24301 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
    24361 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
    24421 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
    24481 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
    24541 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
    24601 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
    24661 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
    24721 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
    24781 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
    24841 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
    24901 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
    24961 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
    25021 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
    25081 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
    25141 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
    25201 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
    25261 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
    25321 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
    25381 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
    25441 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
    25501 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
    25561 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
    25621 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
    25681 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
    25741 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
    25801 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
    25861 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
    25921 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
    25981 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
    26041 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
    26101 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
    26161 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
    26221 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
    26281 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
    26341 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
    26401 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
    26461 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
    26521 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
    26581 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
    26641 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
    26701 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
    26761 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
    26821 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
    26881 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
    26941 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
    27001 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
    27061 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
    27121 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
    27181 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
    27241 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
    27301 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
    27361 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
    27421 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
    27481 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
    27541 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
    27601 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
    27661 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
    27721 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
    27781 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
    27841 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
    27901 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
    27961 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
    28021 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
    28081 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
    28141 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
    28201 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
    28261 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
    28321 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
    28381 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
    28441 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
    28501 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
    28561 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
    28621 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
    28681 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
    28741 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
    28801 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
    28861 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
    28921 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
    28981 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
    29041 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
    29101 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
    29161 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
    29221 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    29281 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    29341 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    29401 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    29461 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    29521 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    29581 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    29641 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    29701 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    29761 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    29821 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    29881 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    29941 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    30001 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    30061 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    30121 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    30181 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    30241 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    30301 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    30361 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    30421 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    30481 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    30541 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    30601 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    30661 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    30721 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    30781 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    30841 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    30901 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    30961 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    31021 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    31081 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    31141 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    31201 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    31261 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    31321 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    31381 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    31441 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    31501 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    31561 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    31621 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    31681 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    31741 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    31801 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    31861 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    31921 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    31981 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    32041 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    32101 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    32161 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    32221 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    32281 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    32341 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    32401 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    32461 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    32521 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    32581 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    32641 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    32701 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    32761 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    32821 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    32881 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    32941 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    33001 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    33061 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    33121 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    33181 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    33241 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    33301 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    33361 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    33421 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    33481 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    33541 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    33601 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    33661 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    33721 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    33781 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    33841 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    33901 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    33961 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    34021 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    34081 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    34141 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    34201 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    34261 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    34321 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    34381 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    34441 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    34501 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    34561 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    34621 attataatga tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DACIFS010000030        34562 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.030, whole genome shotgun sequence.
ACCESSION   DACIFS010000030 DACIFS010000000
VERSION     DACIFS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.030"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            517..1206
                     /locus_tag="IYA83_001425"
     CDS             517..1206
                     /locus_tag="IYA83_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586865.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1193..2401
                     /locus_tag="IYA83_001426"
     CDS             1193..2401
                     /locus_tag="IYA83_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8586866.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2695..3966
                     /gene="serS"
                     /locus_tag="IYA83_001427"
     CDS             2695..3966
                     /gene="serS"
                     /locus_tag="IYA83_001427"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ8586867.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4515..5999)
                     /gene="guaB"
                     /locus_tag="IYA83_001428"
     CDS             complement(4515..5999)
                     /gene="guaB"
                     /locus_tag="IYA83_001428"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ8586868.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6504..7199)
                     /locus_tag="IYA83_001429"
     CDS             complement(6504..7199)
                     /locus_tag="IYA83_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ8586869.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7289..8389)
                     /gene="ychF"
                     /locus_tag="IYA83_001430"
     CDS             complement(7289..8389)
                     /gene="ychF"
                     /locus_tag="IYA83_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ8586870.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8398..8589)
                     /locus_tag="IYA83_001431"
     CDS             complement(8398..8589)
                     /locus_tag="IYA83_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ8586871.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8610..9500)
                     /locus_tag="IYA83_001432"
     CDS             complement(8610..9500)
                     /locus_tag="IYA83_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ8586872.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9487..10254)
                     /locus_tag="IYA83_001433"
     CDS             complement(9487..10254)
                     /locus_tag="IYA83_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ8586873.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10266..10979)
                     /gene="rsmG"
                     /locus_tag="IYA83_001434"
     CDS             complement(10266..10979)
                     /gene="rsmG"
                     /locus_tag="IYA83_001434"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ8586874.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11211..13112)
                     /gene="mnmG"
                     /locus_tag="IYA83_001435"
     CDS             complement(11211..13112)
                     /gene="mnmG"
                     /locus_tag="IYA83_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ8586875.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13129..14526)
                     /gene="mnmE"
                     /locus_tag="IYA83_001436"
     CDS             complement(13129..14526)
                     /gene="mnmE"
                     /locus_tag="IYA83_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ8586876.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14870..15208
                     /locus_tag="IYA83_001437"
     CDS             14870..15208
                     /locus_tag="IYA83_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586877.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15376..16134)
                     /locus_tag="IYA83_001438"
     CDS             complement(15376..16134)
                     /locus_tag="IYA83_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ8586878.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16152..16970)
                     /locus_tag="IYA83_001439"
     CDS             complement(16152..16970)
                     /locus_tag="IYA83_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ8586879.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16967..17323)
                     /gene="rnpA"
                     /locus_tag="IYA83_001440"
     CDS             complement(16967..17323)
                     /gene="rnpA"
                     /locus_tag="IYA83_001440"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ8586880.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17441..17575)
                     /gene="rpmH"
                     /locus_tag="IYA83_001441"
     CDS             complement(17441..17575)
                     /gene="rpmH"
                     /locus_tag="IYA83_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ8586881.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18098..19432
                     /gene="dnaA"
                     /locus_tag="IYA83_001442"
     CDS             18098..19432
                     /gene="dnaA"
                     /locus_tag="IYA83_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ8586882.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19630..20760
                     /gene="dnaN"
                     /locus_tag="IYA83_001443"
     CDS             19630..20760
                     /gene="dnaN"
                     /locus_tag="IYA83_001443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ8586883.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20989..21231
                     /gene="yaaA"
                     /locus_tag="IYA83_001444"
     CDS             20989..21231
                     /gene="yaaA"
                     /locus_tag="IYA83_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ8586884.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21218..22342
                     /gene="recF"
                     /locus_tag="IYA83_001445"
     CDS             21218..22342
                     /gene="recF"
                     /locus_tag="IYA83_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ8586885.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22339..24285
                     /gene="gyrB"
                     /locus_tag="IYA83_001446"
     CDS             22339..24285
                     /gene="gyrB"
                     /locus_tag="IYA83_001446"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ8586886.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24305..26776
                     /gene="gyrA"
                     /locus_tag="IYA83_001447"
     CDS             24305..26776
                     /gene="gyrA"
                     /locus_tag="IYA83_001447"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ8586887.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27012..27311
                     /locus_tag="IYA83_001448"
     CDS             27012..27311
                     /locus_tag="IYA83_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ8586888.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27361..27873
                     /gene="ssb"
                     /locus_tag="IYA83_001449"
     CDS             27361..27873
                     /gene="ssb"
                     /locus_tag="IYA83_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ8586889.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27899..28135
                     /gene="rpsR"
                     /locus_tag="IYA83_001450"
     CDS             27899..28135
                     /gene="rpsR"
                     /locus_tag="IYA83_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ8586890.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28278..30251
                     /locus_tag="IYA83_001451"
     CDS             28278..30251
                     /locus_tag="IYA83_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ8586891.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFTAIKENMNQIYDKE"
     gene            30257..30709
                     /gene="rplI"
                     /locus_tag="IYA83_001452"
     CDS             30257..30709
                     /gene="rplI"
                     /locus_tag="IYA83_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ8586892.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30983..32350
                     /gene="dnaB"
                     /locus_tag="IYA83_001453"
     CDS             30983..32350
                     /gene="dnaB"
                     /locus_tag="IYA83_001453"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ8586893.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33130..34422
                     /locus_tag="IYA83_001454"
     CDS             33130..34422
                     /locus_tag="IYA83_001454"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ8586894.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10535 a         6719 c         6828 g        10480 t
ORIGIN      
        1 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
       61 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      121 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      181 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      241 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      301 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      361 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      421 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      481 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      541 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
      601 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
      661 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
      721 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
      781 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
      841 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
      901 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
      961 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1021 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1081 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1141 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1201 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1261 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1321 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1381 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1441 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1501 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1561 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     1621 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     1681 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     1741 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     1801 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     1861 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     1921 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     1981 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2041 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2101 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2161 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2221 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2281 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2341 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2401 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2461 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2521 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     2581 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     2641 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     2701 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     2761 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     2821 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     2881 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     2941 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3001 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3061 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3121 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3181 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3241 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3301 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3361 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3421 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3481 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3541 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     3601 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     3661 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     3721 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     3781 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     3841 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     3901 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     3961 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4021 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4081 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4141 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4201 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4261 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4321 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4381 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4441 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4501 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4561 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     4621 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     4681 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     4741 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     4801 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     4861 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     4921 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     4981 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5041 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5101 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5161 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5221 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca
     5281 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5341 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5401 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5461 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5521 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     5581 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     5641 tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa gaagaacgga
     5701 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     5761 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     5821 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     5881 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     5941 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6001 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6061 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6121 tctgtgtata ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6181 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6241 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6301 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6361 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6421 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6481 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6541 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     6601 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     6661 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     6721 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     6781 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     6841 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     6901 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     6961 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7021 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7081 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7141 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7201 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7261 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7321 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7381 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7441 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7501 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7561 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     7621 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     7681 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     7741 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     7801 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     7861 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     7921 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     7981 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8041 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8101 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8161 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8221 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8281 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8341 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8401 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8461 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8521 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     8581 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     8641 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     8701 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     8761 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     8821 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     8881 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     8941 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9001 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9061 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9121 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9181 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9241 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9301 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9361 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9421 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9481 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9541 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
     9601 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
     9661 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
     9721 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
     9781 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
     9841 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
     9901 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
     9961 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10021 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10081 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10141 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10201 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10261 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10321 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10381 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10441 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10501 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10561 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    10621 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    10681 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    10741 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    10801 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    10861 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    10921 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    10981 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11041 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11101 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11161 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11221 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11281 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11341 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11401 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11461 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11521 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    11581 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    11641 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    11701 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    11761 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    11821 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    11881 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    11941 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12001 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12061 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12121 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12181 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12241 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12301 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12361 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12421 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12481 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12541 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    12601 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    12661 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    12721 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    12781 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    12841 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    12901 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    12961 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13021 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13081 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13141 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13201 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13261 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13321 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13381 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13441 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13501 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13561 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    13621 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    13681 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    13741 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    13801 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    13861 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    13921 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    13981 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14041 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14101 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14161 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14221 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14281 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14341 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14401 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14461 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14521 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    14581 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    14641 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    14701 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    14761 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    14821 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    14881 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    14941 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15001 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15061 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15121 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15181 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15241 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15301 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15361 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15421 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15481 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15541 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    15601 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    15661 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    15721 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    15781 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    15841 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    15901 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    15961 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16021 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16081 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16141 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16201 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16261 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16321 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16381 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16441 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16501 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16561 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    16621 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    16681 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    16741 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    16801 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    16861 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    16921 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    16981 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17041 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17101 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17161 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17221 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17281 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17341 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17401 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17461 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17521 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    17581 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    17641 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    17701 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    17761 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    17821 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    17881 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    17941 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18001 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18061 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18121 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18181 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18241 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18301 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18361 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18421 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18481 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18541 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    18601 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    18661 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    18721 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    18781 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    18841 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    18901 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    18961 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19021 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19081 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19141 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19201 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19261 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19321 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19381 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19441 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19501 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19561 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    19621 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    19681 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    19741 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    19801 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    19861 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    19921 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    19981 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20041 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20101 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20161 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20221 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20281 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20341 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20401 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20461 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20521 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    20581 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    20641 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    20701 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    20761 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    20821 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
    20881 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa
    20941 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
    21001 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
    21061 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
    21121 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
    21181 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
    21241 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
    21301 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
    21361 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
    21421 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
    21481 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
    21541 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
    21601 gcagaccagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
    21661 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
    21721 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
    21781 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
    21841 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
    21901 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
    21961 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
    22021 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
    22081 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
    22141 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
    22201 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
    22261 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
    22321 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
    22381 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
    22441 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
    22501 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
    22561 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
    22621 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
    22681 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt taacgcgctt
    22741 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
    22801 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
    22861 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
    22921 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
    22981 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gattaaaagt
    23041 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
    23101 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
    23161 aatatcatga gttttgccaa caatatccat acgtatgaag gcggaacaca tgaatcagga
    23221 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
    23281 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
    23341 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
    23401 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
    23461 ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
    23521 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
    23581 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
    23641 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
    23701 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
    23761 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
    23821 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
    23881 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
    23941 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
    24001 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
    24061 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
    24121 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
    24181 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
    24241 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
    24301 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
    24361 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
    24421 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
    24481 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
    24541 tcctcatgga gactatgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
    24601 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
    24661 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
    24721 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
    24781 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
    24841 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
    24901 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
    24961 aacaggtggt ttggttatgg gaaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
    25021 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
    25081 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
    25141 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
    25201 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatctgtta tcttgaataa
    25261 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
    25321 aggtgtgccg aaagttttga gcttgaaaca aattcttgaa aactatatcg aacatcagcg
    25381 cgaagtcatc acaagacgga cagtctttga aaaagaaaaa gcagaagcaa gggcacacat
    25441 tttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
    25501 gtctaaatca gatgacgaag caaaagcgac gatgatcgaa cggttcgatt tatccgatcg
    25561 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
    25621 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
    25681 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
    25741 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
    25801 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
    25861 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
    25921 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
    25981 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
    26041 cacagccaaa ggaataccaa ttatcaattt actaggtatc gattcgagtg aaagcatcca
    26101 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
    26161 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
    26221 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
    26281 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
    26341 ccgcgatatg ggacgtacag cttccggggt tagaggaata cgcctgcgtg aggaagatta
    26401 tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta ttagtgatca cagaaaaagg
    26461 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
    26521 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
    26581 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
    26641 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
    26701 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
    26761 aggatcagaa gaatagccat caagactaat tgtgtaagct agcaagaatg accgattaaa
    26821 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
    26881 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aatcagagct tgtgagtagt
    26941 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
    27001 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
    27061 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
    27121 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
    27181 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
    27241 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
    27301 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
    27361 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
    27421 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
    27481 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
    27541 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
    27601 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
    27661 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
    27721 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
    27781 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
    27841 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
    27901 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
    27961 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
    28021 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
    28081 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
    28141 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
    28201 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
    28261 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
    28321 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
    28381 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
    28441 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
    28501 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
    28561 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
    28621 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
    28681 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
    28741 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
    28801 ttttatctat tgataactat gcagatgcca ttgataaatt ggatgacaaa gagatttctt
    28861 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
    28921 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
    28981 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
    29041 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
    29101 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
    29161 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
    29221 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
    29281 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
    29341 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaacgaga
    29401 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
    29461 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
    29521 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
    29581 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
    29641 tgacttatat cgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
    29701 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
    29761 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
    29821 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
    29881 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
    29941 ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca gcaaaaacag
    30001 cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca aagcggacag
    30061 acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa atcatcatgg
    30121 aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa aatacgacag
    30181 ttgaggaaac aaaacagaag ctatttactg caatcaaaga aaatatgaat caaatatatg
    30241 ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa aaggtaaaaa
    30301 aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca aaaataactt
    30361 ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga aagcacaaga
    30421 aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct tggaaaaaga
    30481 agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt ttggttcgat
    30541 cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat tagataaacg
    30601 aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac ctgttaagct
    30661 tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac aaatcttgat
    30721 agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa atttatctga
    30781 gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg atttcggttt
    30841 cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga gaactatttt
    30901 tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa aatgaagcaa
    30961 ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc caccgcaaaa
    31021 tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa cgatcattga
    31081 tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa ttatttttga
    31141 gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg tcaaagaccg
    31201 gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct cagacttggc
    31261 attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg aagaaaaatc
    31321 attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaagggt tcgagcaagg
    31381 agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg tttcggaaaa
    31441 aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga ttgaaaatat
    31501 cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct atcaaggtct
    31561 ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg cacgtccagc
    31621 ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa aaacagatcg
    31681 ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc ggatgctttg
    31741 tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg aagaagaatg
    31801 gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta tcgatgatac
    31861 accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc aagaaaaagg
    31921 caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg gtaaagagaa
    31981 ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga aagagctgaa
    32041 agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac aagacaaacg
    32101 accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg acatcgtagc
    32161 cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg atggaaacga
    32221 gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg gtgcacgagg
    32281 aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt cgccaagaga
    32341 agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg taagaagatg
    32401 caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc aaagggattg
    32461 tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc agctgaccct
    32521 tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg tttctctaat
    32581 actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc tcctgccaac
    32641 tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga aattgctcct
    32701 tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt tcaaaagctt
    32761 gcatctgcgg taatacgccc aatcaaacga aaaatatgag aagctatttt tgtttggctg
    32821 ttcttattac ttgatgcagg acagcttttt cacaacctct gattcacggt gttccaaaag
    32881 tagcttcttc gaaaaataag tcgaaatttc acaaaaattt gagaagcaat tttcgtacat
    32941 tccttcttat ttcttgaagc tgaccacttc tgttacaacc tcttttttaa taaccattct
    33001 aaaaagataa agttcgtttt ttaagcgttt ttataaaata aaaataaaaa tcattcgatt
    33061 ttatttgaaa tatgtatcta ttattgatac aatggttcag gattaaataa tatagaacga
    33121 ggtgttcaca tgtcatctgt agtagtagta ggtacgcaat ggggcgatga agggaaagga
    33181 aaaatcacag actttctcag tgaaaatgct gaagtgattg cacggtacca aggtggcgac
    33241 aatgcagggc acacaattaa gtttaacggt gtgacttata aactgcattt gattccttct
    33301 gggatttttt ataaagacaa gatcagtgtg atcggaaacg gcgttgtagt caatcctaaa
    33361 tcattggtta aagaattagc ttatttacat gaaaaaggtg tagaaacaag taatttgcgt
    33421 atttctgacc gcgcgcacgt aatcttgcct tatcatatca aattggacca attacaagaa
    33481 gatgctaagg gcgaaaataa gattggtaca acgattaaag gaatcggacc tgcctatatg
    33541 gataaagcag ctcgtgtagg tatccgtatt gcagacttat tagataaaga aatttttgaa
    33601 gaacgtcttc gtttgaatct agatgacaaa aaccgtcaat ttgtaaaaat gtttgattca
    33661 gaaccacttg attttgacga tatttttgaa gaatattatg aatacggaca acaaatcaaa
    33721 caatacgtca cagatacttc cgttatcttg aatgatgctt tggatgcagg caaacgcgtg
    33781 ttgtttgaag gcgcacaagg tgtaatgctg gatatcgacc agggaactta tccttttgtt
    33841 acttcttcta acccagtagc cggtggggta acaatcggaa gcggtgtagg gccttcaaaa
    33901 atcaataaag ttgtcggcgt ctgcaaagcc tatacgtcac gtgtcggtga tggaccattt
    33961 ccaactgaat tatttgatga aacaggagaa caaatccgcg aagtaggacg agaatacggt
    34021 acgacaactg gtcgtccgcg tcgtgtaggc tggtttgatt ctgtagttat gcgccattct
    34081 aaacgagttt caggtattac caacctatct ttaaactcga tcgatgtatt aagtggattg
    34141 gaaacagtaa aaatttgtac ggcttatgaa ttagatggcg aactaatcta ccactatcca
    34201 gcaagcttga aagagttgaa ccgctgcaaa ccaatctatg aagaattgcc aggctggtca
    34261 gaagacatta caggatgcaa aacacttgca gaactgccag aaaacgcacg taactatgta
    34321 cgtcgtatct cagagcttgt tggtgtacgt atctcaacat tttctgtcgg acctgataga
    34381 acacaaacaa acatcttaga aagcgtctgg gcgcaaatct aaggataaaa acgacgactg
    34441 aatcaggacg ttcttaccag agcgagtcga agtgtttatc tatacgataa taataacttg
    34501 aacactgttt attcagaata agggactgtg acaaaaaccg tgtcacagtc cctttttccg
    34561 aa
//
LOCUS       DACIFS010000031        31372 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.031, whole genome shotgun sequence.
ACCESSION   DACIFS010000031 DACIFS010000000
VERSION     DACIFS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.031"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            271..1140
                     /locus_tag="IYA83_001455"
     CDS             271..1140
                     /locus_tag="IYA83_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ8586895.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1349..2374
                     /locus_tag="IYA83_001456"
     CDS             1349..2374
                     /locus_tag="IYA83_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ8586896.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2413..3363
                     /gene="manA"
                     /locus_tag="IYA83_001457"
     CDS             2413..3363
                     /gene="manA"
                     /locus_tag="IYA83_001457"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ8586897.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3457..5160
                     /locus_tag="IYA83_001458"
     CDS             3457..5160
                     /locus_tag="IYA83_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8586898.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5162..7195
                     /locus_tag="IYA83_001459"
     CDS             5162..7195
                     /locus_tag="IYA83_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ8586899.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7206..7646
                     /locus_tag="IYA83_001460"
     CDS             7206..7646
                     /locus_tag="IYA83_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8586900.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7846..8973)
                     /locus_tag="IYA83_001461"
     CDS             complement(7846..8973)
                     /locus_tag="IYA83_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586901.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8976..9653)
                     /locus_tag="IYA83_001462"
     CDS             complement(8976..9653)
                     /locus_tag="IYA83_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586902.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9917..11683
                     /locus_tag="IYA83_001463"
     CDS             9917..11683
                     /locus_tag="IYA83_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ8586903.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11698..12135
                     /locus_tag="IYA83_001464"
     CDS             11698..12135
                     /locus_tag="IYA83_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8586904.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12148..13299
                     /locus_tag="IYA83_001465"
     CDS             12148..13299
                     /locus_tag="IYA83_001465"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ8586905.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13361..14059)
                     /locus_tag="IYA83_001466"
     CDS             complement(13361..14059)
                     /locus_tag="IYA83_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ8586906.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14052..15404)
                     /locus_tag="IYA83_001467"
     CDS             complement(14052..15404)
                     /locus_tag="IYA83_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ8586907.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15627..17027)
                     /locus_tag="IYA83_001468"
     CDS             complement(15627..17027)
                     /locus_tag="IYA83_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ8586908.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17188..17925
                     /locus_tag="IYA83_001469"
     CDS             17188..17925
                     /locus_tag="IYA83_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ8586909.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18050..18529
                     /locus_tag="IYA83_001470"
     CDS             18050..18529
                     /locus_tag="IYA83_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ8586910.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18526..19293
                     /locus_tag="IYA83_001471"
     CDS             18526..19293
                     /locus_tag="IYA83_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586911.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19523..20092
                     /gene="lepB"
                     /locus_tag="IYA83_001472"
     CDS             19523..20092
                     /gene="lepB"
                     /locus_tag="IYA83_001472"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ8586912.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20196..20798)
                     /locus_tag="IYA83_001473"
     CDS             complement(20196..20798)
                     /locus_tag="IYA83_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ8586913.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20904..21373
                     /locus_tag="IYA83_001474"
                     /pseudo
     CDS             20904..21373
                     /locus_tag="IYA83_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21479..22063
                     /locus_tag="IYA83_001475"
     CDS             21479..22063
                     /locus_tag="IYA83_001475"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ8586914.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22066..23139
                     /gene="prfA"
                     /locus_tag="IYA83_001476"
     CDS             22066..23139
                     /gene="prfA"
                     /locus_tag="IYA83_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ8586915.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23132..23971
                     /gene="prmC"
                     /locus_tag="IYA83_001477"
     CDS             23132..23971
                     /gene="prmC"
                     /locus_tag="IYA83_001477"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ8586916.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23964..24143
                     /locus_tag="IYA83_001478"
     CDS             23964..24143
                     /locus_tag="IYA83_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586917.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24165..25190
                     /locus_tag="IYA83_001479"
     CDS             24165..25190
                     /locus_tag="IYA83_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ8586918.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25365..26609
                     /locus_tag="IYA83_001480"
     CDS             25365..26609
                     /locus_tag="IYA83_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ8586919.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26762..27709
                     /locus_tag="IYA83_001481"
     CDS             26762..27709
                     /locus_tag="IYA83_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8586920.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27843..28472
                     /gene="upp"
                     /locus_tag="IYA83_001482"
     CDS             27843..28472
                     /gene="upp"
                     /locus_tag="IYA83_001482"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ8586921.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28611..28853)
                     /locus_tag="IYA83_001483"
     CDS             complement(28611..28853)
                     /locus_tag="IYA83_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586922.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28974..29046
                     /locus_tag="IYA83_001484"
     tRNA            28974..29046
                     /locus_tag="IYA83_001484"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29007..29009,aa:Thr,seq:cgt)
     gene            complement(29165..29803)
                     /locus_tag="IYA83_001485"
     CDS             complement(29165..29803)
                     /locus_tag="IYA83_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ8586923.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29926..30558)
                     /locus_tag="IYA83_001486"
     CDS             complement(29926..30558)
                     /locus_tag="IYA83_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ8586924.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30909..31208
                     /locus_tag="IYA83_001487"
     CDS             30909..31208
                     /locus_tag="IYA83_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ8586925.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10699 a         5479 c         6314 g         8880 t
ORIGIN      
        1 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
       61 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
      121 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      181 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      241 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      301 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      361 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      421 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      481 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      541 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      601 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      661 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      721 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      781 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      841 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      901 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      961 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
     1021 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1081 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1141 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1201 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1261 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1321 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1381 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1441 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1501 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1561 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1621 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1681 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1741 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1801 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1861 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1921 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1981 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     2041 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2101 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2161 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2221 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2281 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2341 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2401 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2461 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2521 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2581 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2641 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2701 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2761 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2821 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2881 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2941 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     3001 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3061 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3121 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3181 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3241 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3301 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3361 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3421 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3481 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3541 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3601 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3661 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3721 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3781 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3841 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3901 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3961 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     4021 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4081 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4141 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4201 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4261 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4321 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4381 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4441 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4501 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4561 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4621 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4681 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4741 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4801 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4861 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4921 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4981 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     5041 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5101 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5161 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5221 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5281 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5341 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5401 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5461 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5521 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5581 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5641 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5701 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5761 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5821 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5881 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5941 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     6001 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6061 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6121 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6181 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6241 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6301 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6361 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6421 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6481 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6541 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6601 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6661 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6721 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6781 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6841 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6901 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6961 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     7021 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7081 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7141 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7201 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7261 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7321 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7381 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7441 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7501 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7561 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7621 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7681 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7741 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7801 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7861 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7921 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7981 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     8041 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8101 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8161 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8221 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8281 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8341 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8401 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8461 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8521 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8581 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8641 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8701 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8761 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8821 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8881 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8941 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     9001 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9061 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9121 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9181 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9241 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9301 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9361 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9421 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9481 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9541 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9601 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9661 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9721 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9781 tttctgatac cgaaatcttt ttattttgtt gacatactca aacctttggc acaaagtttc
     9841 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9901 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9961 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    10021 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10081 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10141 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10201 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10261 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10321 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10381 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10441 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10501 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10561 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10621 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10681 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10741 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10801 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10861 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10921 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10981 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    11041 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11101 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11161 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11221 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11281 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11341 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11401 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11461 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11521 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11581 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11641 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11701 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11761 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11821 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11881 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11941 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    12001 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12061 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12121 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12181 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12241 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12301 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12361 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12421 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12481 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12541 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12601 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12661 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12721 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12781 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12841 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12901 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12961 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    13021 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13081 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13141 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13201 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13261 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13321 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13381 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13441 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13501 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13561 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13621 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13681 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13741 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13801 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13861 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13921 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13981 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    14041 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14101 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14161 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14221 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14281 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14341 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14401 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14461 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14521 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14581 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14641 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14701 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14761 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14821 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14881 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14941 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    15001 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15061 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15121 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15181 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15241 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15301 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15361 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15421 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15481 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15541 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15601 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15661 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15721 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15781 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15841 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15901 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15961 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    16021 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16081 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16141 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16201 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16261 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16321 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16381 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16441 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16501 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16561 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16621 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16681 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16741 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16801 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16861 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16921 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16981 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    17041 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17101 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17161 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17221 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17281 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17341 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17401 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17461 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17521 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17581 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17641 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17701 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17761 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17821 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17881 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17941 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    18001 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18061 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18121 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18181 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18241 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18301 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18361 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18421 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18481 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18541 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18601 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18661 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18721 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18781 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18841 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18901 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18961 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    19021 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19081 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19141 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19201 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19261 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19321 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19381 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19441 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19501 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19561 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19621 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19681 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19741 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19801 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19861 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19921 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19981 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    20041 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20101 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20161 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20221 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20281 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20341 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20401 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20461 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20521 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20581 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20641 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20701 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20761 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20821 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20881 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20941 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    21001 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21061 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21121 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21181 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21241 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21301 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21361 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21421 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21481 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21541 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21601 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21661 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21721 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21781 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21841 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21901 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21961 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    22021 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22081 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22141 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22201 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22261 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22321 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22381 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22441 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22501 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22561 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22621 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22681 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22741 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22801 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22861 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22921 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22981 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    23041 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23101 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23161 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23221 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23281 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23341 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23401 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23461 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23521 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23581 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23641 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23701 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23761 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23821 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23881 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23941 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    24001 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24061 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24121 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24181 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24241 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24301 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24361 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24421 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24481 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24541 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24601 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24661 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24721 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24781 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24841 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24901 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24961 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    25021 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25081 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25141 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25201 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25261 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25321 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25381 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25441 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25501 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25561 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25621 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25681 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25741 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25801 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25861 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25921 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25981 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    26041 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26101 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26161 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26221 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26281 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26341 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26401 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26461 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26521 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26581 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26641 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26701 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26761 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26821 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26881 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26941 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    27001 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27061 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27121 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27181 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27241 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27301 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27361 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27421 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27481 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27541 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27601 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27661 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27721 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27781 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27841 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27901 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27961 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    28021 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28081 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28141 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28201 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28261 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28321 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28381 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28441 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28501 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28561 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28621 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28681 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28741 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28801 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28861 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28921 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28981 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    29041 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29101 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29161 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29221 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29281 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29341 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29401 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29461 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29521 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29581 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29641 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29701 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29761 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29821 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29881 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29941 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    30001 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30061 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30121 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30181 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30241 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30301 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30361 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30421 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30481 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30541 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30601 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30661 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30721 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30781 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30841 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30901 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30961 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    31021 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31081 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31141 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31201 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31261 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31321 tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt at
//
LOCUS       DACIFS010000032        31115 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.032, whole genome shotgun sequence.
ACCESSION   DACIFS010000032 DACIFS010000000
VERSION     DACIFS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.032"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(117..707)
                     /locus_tag="IYA83_001488"
     CDS             complement(117..707)
                     /locus_tag="IYA83_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ8586926.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="IYA83_001489"
     CDS             1230..1391
                     /locus_tag="IYA83_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586927.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="IYA83_001490"
     CDS             1410..1904
                     /locus_tag="IYA83_001490"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAQ8586928.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2542..3565
                     /locus_tag="IYA83_001491"
                     /pseudo
     CDS             2542..3565
                     /locus_tag="IYA83_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3908..4164
                     /locus_tag="IYA83_001492"
                     /pseudo
     CDS             3908..4164
                     /locus_tag="IYA83_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4551..4778
                     /locus_tag="IYA83_001493"
     CDS             4551..4778
                     /locus_tag="IYA83_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ8586929.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4794..5960
                     /locus_tag="IYA83_001494"
     CDS             4794..5960
                     /locus_tag="IYA83_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ8586930.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6195..7238
                     /gene="hrcA"
                     /locus_tag="IYA83_001495"
     CDS             6195..7238
                     /gene="hrcA"
                     /locus_tag="IYA83_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ8586931.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7332..7895
                     /gene="grpE"
                     /locus_tag="IYA83_001496"
     CDS             7332..7895
                     /gene="grpE"
                     /locus_tag="IYA83_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ8586932.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7946..9775
                     /gene="dnaK"
                     /locus_tag="IYA83_001497"
     CDS             7946..9775
                     /gene="dnaK"
                     /locus_tag="IYA83_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ8586933.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9926..11092
                     /gene="dnaJ"
                     /locus_tag="IYA83_001498"
     CDS             9926..11092
                     /gene="dnaJ"
                     /locus_tag="IYA83_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ8586934.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11460..12197)
                     /locus_tag="IYA83_001499"
     CDS             complement(11460..12197)
                     /locus_tag="IYA83_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586935.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12199..13701)
                     /locus_tag="IYA83_001500"
     CDS             complement(12199..13701)
                     /locus_tag="IYA83_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8586936.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13960..15954
                     /gene="uvrB"
                     /locus_tag="IYA83_001501"
     CDS             13960..15954
                     /gene="uvrB"
                     /locus_tag="IYA83_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ8586937.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            15964..18780
                     /gene="uvrA"
                     /locus_tag="IYA83_001502"
     CDS             15964..18780
                     /gene="uvrA"
                     /locus_tag="IYA83_001502"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ8586938.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19043..20308
                     /locus_tag="IYA83_001503"
     CDS             19043..20308
                     /locus_tag="IYA83_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ8586939.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20312..21043
                     /locus_tag="IYA83_001504"
     CDS             20312..21043
                     /locus_tag="IYA83_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ8586940.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21178..22062
                     /gene="rapZ"
                     /locus_tag="IYA83_001505"
     CDS             21178..22062
                     /gene="rapZ"
                     /locus_tag="IYA83_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ8586941.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22059..23057
                     /locus_tag="IYA83_001506"
     CDS             22059..23057
                     /locus_tag="IYA83_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ8586942.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23091..24026
                     /gene="whiA"
                     /locus_tag="IYA83_001507"
     CDS             23091..24026
                     /gene="whiA"
                     /locus_tag="IYA83_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ8586943.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24594..25250
                     /locus_tag="IYA83_001508"
     CDS             24594..25250
                     /locus_tag="IYA83_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586944.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25429..25994
                     /locus_tag="IYA83_001509"
                     /pseudo
     CDS             25429..25994
                     /locus_tag="IYA83_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26693..27214)
                     /locus_tag="IYA83_001510"
     CDS             complement(26693..27214)
                     /locus_tag="IYA83_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ8586945.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27412..31092
                     /gene="nifJ"
                     /locus_tag="IYA83_001511"
     CDS             27412..31092
                     /gene="nifJ"
                     /locus_tag="IYA83_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ8586946.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
BASE COUNT        10396 a         5205 c         6624 g         8890 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaaacacag ctaaaaatta
     2161 gttgtgtttt ttattaggtt tacttttctg gtataaaagg aactagctac tttttcaaaa
     2221 tttctaaatc attcaatatg tagcgcttac taaattattc gccataaaat tttataattg
     2281 gatcactcat tgttttacct aagtcatatt taagtaataa ccggtgtttt tacataagaa
     2341 gacttcgggt attttttagt ttgattagct ctgatatacc tataaagaat atgctaaggt
     2401 aagaatagta aaaattcaca tagatgaggt ataataatga tatttttata tattacagat
     2461 aacttgacac aaaatgagtt tttttcgatt tttcaagtta tctaatgcaa ttatattgat
     2521 atcattagga gctgcattgt tatgaatcca acattgatta ctaagaaaga attactaaaa
     2581 aaattagata tttctactgg tgtattagct aatttaatta gaaatggcat gccaaaagag
     2641 ggagaaatgt ttaatcttga taagattatt acatggcgag aaaattggtc taaaaacata
     2701 ttaggtgagt tagaagttgg tagagtatat actaacaaag aaatttcaga gaagtttaaa
     2761 tgttccaaac agggtggaat gagaagatct catcaaacta atactcttgt actgtttagt
     2821 gatcaaacag gaagtaatgt ttataaagat aagtggttaa atggtatttt acaatatacc
     2881 ggtatggggc taaaagggga tcaagtattg gataagaatc aaaataaagt tttagccaat
     2941 tcaaaaagta attttgtaaa gattcattta tttgaaacat ttaaaccaaa agaacacaca
     3001 tatctgggag aagtatattt agcaggacaa atatatactg ttaatgaaaa agatagttcg
     3061 ggcaacagca gaaaagtata taaatttcca ttagctttaa taaatcaaga gcaattgatt
     3121 gaagataaag atatataacc aggaagaaaa tcaaactaga catattcgta atttatctaa
     3181 tgctaaatta gaggaagaag caagaaaagt tagtaattat aataaaaaaa tggctgcaaa
     3241 atatgttaac gaagaggagt tttgcttctc aaaaacaaaa atatataaac gtaatccctt
     3301 cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt caactttgcc aagaaaaagg
     3361 gccatttata aaagacggtg taccttattt acattgtcat catatagaat atttgtcgca
     3421 aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt cccaattgtc atgctagaat
     3481 tcatgaactt gagttacctg acgataaaga aaaattgctg aagatcgttg aaaaaagaga
     3541 aaatagcttt tctaaaatac cgtaatttat ttttatagga caaataagct aatgtgagct
     3601 atgccttcta tataagacta agttctatta taagtaacga agtaattctc gttccttcct
     3661 gtagtaatat tttttaaata acctataggc ttcttcaaaa gtacatcctc cagtaaattc
     3721 taagtactgg aggattcttt ttctagtgtt gctttactca tgaaaaagta gttggctact
     3781 tcatcaaaat ttctgaactt ttttaatgta taataaagag ctcgatcatt tactacaagg
     3841 atgctagctc ctacattttc acagtttttt agcaagtaaa tttttctaaa atcacatcag
     3901 gaaatgcatg agattcttta agagaggcaa ttatagggct atgattacgg ttcgtgtaag
     3961 tgacgccaaa aaagaatggc tttcttatat ggctgaattt tatgggatta ctttatctga
     4021 acttctaaaa aactattcga tggaacaact agaagatgaa tacaatcgtc aaacggctga
     4081 aattgcccat aaacattgga taagaagtgg gaaaacaaca gtattcatgg aagaatcctt
     4141 aatgaatttg gtgggctgga atgacctgtc gtttaaaatt cacgcccgaa attcagaagc
     4201 agctctgatt ggcaaatacg tgtattggta gagatagaag ataatcaatt cgttgttatc
     4261 gctattcaag tagggcaccg aagaaatgtc tatgattaag agactgttta tttttacaaa
     4321 aaaataataa ttggaatcta gttaaaactc ttgaagtgag agatttgact agattttttt
     4381 atgaataaaa taaggacaaa attgtttagg aagaatcatg agtgtaaaaa aatcatccaa
     4441 gaagtcgatt cttggatttt ttagtggatt tgttcgccaa atacgtttag tatgcgtata
     4501 tgagaggttg taagcaaagc gttgtggtct gagcacttaa ggaggaatct gtgaaaatag
     4561 cttttcatat ttttgcagat tttgacttaa tgccgaaggt cagctttgcg attaccgttt
     4621 atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca tttttagagc
     4681 taggtttcct tgattctaaa tatgcggctt atttcaatga gttggtgctt gaacgcgatt
     4741 ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg agaaagaatt tcaatgatag
     4801 aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat tcatatccca ttttgcgagc
     4861 atatctgcta ttactgtgac tttaataaag tttttttaga agggcagcca gtagatgagt
     4921 atgtggaaat gctgctgaat gaaatgagat taatgctgga aagtcatcct acagcgaatt
     4981 tggaaacact ttatgtagga ggcggaacac caacttcttt atctgctaaa caacttgatc
     5041 gactattatc aggtgctaga gagatcctgc cttttcaaaa tggtaatgag tttactgtag
     5101 aagcgaatcc tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca
     5161 atcgtttatc catgggagtg cagacatttg acaatcgctt attgaaaaaa attggacgta
     5221 aacatactgc agaagatgtg tatcaaacaa tgcaattttt agaagcagaa aattttacca
     5281 atgtaagcat cgatttgatt tatgcattac cgggacaaac actagaagga aatcgggata
     5341 ctttggaaaa agctttggct ttggatctgc ctcactattc tttgtactca ttgatcctcg
     5401 aaaacaaaac aatgttcatg aactgggtac gacaaggaag actcgaactg cctgatcagg
     5461 aaacggaaac cagaatgttt gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc
     5521 aatatgaaat aagtaacttt gggctctccg gacatgaatc tcaacataac ctcatgtact
     5581 ggaacaatga ccactatttt gggtttggag ctggtgctag cggttatctt ggagatattc
     5641 gttaccgcaa caaaggacca atccaacact atttacgccc attacgagca aatgaacgcc
     5701 cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa atgttcttag
     5761 gactaagaaa aaaaacaggg attactaaaa aacatttcta tgatcgctat caaaaaacaa
     5821 ttgaatcggt gtacggtgat gtgctaaggg aattagagca agaagggtta cttgttaatg
     5881 aagaagaccg tgtttatttg acaccaagag ggactttttt ggggaacaat gtgtttgagc
     5941 gctttttatt tgaaaaatag tttgaagaat atttgttttt tatcgaaact tgaaaagaaa
     6001 aagtttagaa agtttgacaa tgaggtattg aagtattgat ataactggat ttacatcaat
     6061 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
     6121 gagaagggct tcttgctata tttataagtg aattagcact tagaatatat gagtgctaat
     6181 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
     6241 actatacgag tagtggcgtg cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg
     6301 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
     6361 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
     6421 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
     6481 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
     6541 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
     6601 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
     6661 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
     6721 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
     6781 ttcgtacgga gattccgatg attcttcata aatatttcca gacgacagaa gggatgtctc
     6841 agttatttga caccgttctt ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa
     6901 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
     6961 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
     7021 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
     7081 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
     7141 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
     7201 ctgattacta ccgctcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
     7261 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
     7321 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
     7381 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
     7441 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
     7501 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
     7561 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
     7621 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
     7681 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
     7741 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
     7801 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
     7861 tgttaagacc aactatggtc attgttgcac aataattgtg cggatataaa acttacactt
     7921 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
     7981 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
     8041 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
     8101 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
     8161 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
     8221 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
     8281 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
     8341 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
     8401 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
     8461 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
     8521 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
     8581 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
     8641 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
     8701 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
     8761 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
     8821 tcaagcgtta aaagatgcag gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg
     8881 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
     8941 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
     9001 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
     9061 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
     9121 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
     9181 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
     9241 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
     9301 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
     9361 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtgaaag atgcagaagc
     9421 aaacgctgaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc
     9481 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
     9541 agtgaaaaaa gcagaaacag ctcgtgacga attaaaagcc gctgttgaag caaacaacat
     9601 cgaggaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
     9661 actatacgaa caagctgcac aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg
     9721 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
     9781 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
     9841 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
     9901 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
     9961 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    10021 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    10081 agcatatgag attttaagtg acccgcaaaa acgcgctgca tacgaccaat acgggcatgc
    10141 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    10201 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    10261 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    10321 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    10381 aatctgtcat acttgtggtg gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    10441 taaatgtcat ggtgcaggta cgatcaatgt agaacgccaa acgccacttg gacgagtgat
    10501 gagccgtcaa acttgtgatg tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc
    10561 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    10621 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    10681 cggaccattt ggtgatttgt atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg
    10741 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    10801 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    10861 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    10921 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    10981 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    11041 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    11101 gtaaaaatat aaagtagact tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt
    11161 aataattaaa aaccagacga acaatcatac gttcgcctgg tttttttgta gttgtcatgt
    11221 ttgtaccaac aaaaaagcca gctttagaat aagataatca tcatagtttc tgaaggtggt
    11281 atcgactatg tacttgatat cgtcaatgaa ccgatggtac tttcttatgc gatggaacag
    11341 gtagagcgat tgcttgtttg tgcaggagaa caagcgatgc ggctggtaca gttaagtgaa
    11401 aaaacaagaa actaaaaatg cccggtcgtc atgaagcgac cgggcatttt tggtttttat
    11461 taaatattta atactttatt caagaaatct tttgtccgtt cattttgcgg attttcaaag
    11521 atttgttgag gcgttccgtc ttctaaaaag tttccgtcgt caatgaacat gacacgattg
    11581 gccacttctt tagcgaagcc catttcgtgc gtaacgataa ccatcgtcat accttgttct
    11641 gctaactttt tcatgacgtt caagacatca ccgaccattt cggggtcgag tgcagatgta
    11701 ggctcatcaa aaagcatgat gtcagggttc atcgctagag cacgagcaat agccacacgc
    11761 tgcttttgtc caccggaaag catttcagga tacatatctt ttttatcttc tagaccaact
    11821 gtctgcagca atttgattgc ttttttctct gcttcttctc tagattcttt cttcaggtcc
    11881 atcggtgcta aaatgatgtt ttccaaaata gaaagatgag ggaataggtt aaagtgctgg
    11941 aataccattc cgatatgttg tctcaccaag ttgatatctg tgcttttatc catcaagtgg
    12001 gcgccgtcaa taataatttc accgtcagaa ggttcttcta aacgattcaa gcaacgcaaa
    12061 aatgtacttt ttcctgaacc tgaagggcca atgatacaga cgacatctcc ttcgttgatg
    12121 gacacattga tgtcgtttaa tactgtgtta tcgccgtatt ttttgactaa atgctcaact
    12181 aagatttttt ctgccatctt acttcacctt cttttctaaa acttttgcta attttgtcaa
    12241 tgctgtgatc aaaatcaagt acatgatcgc aatgatcaag taaacatacg tactttgtgt
    12301 tgtacgtgca acaatgatct taccagtttg cagcaattcg actacaccga ttgcggaaat
    12361 gatcgttgtg tcttttaatg agataacgaa ctgattgacg aatgatggga tcatgatgcg
    12421 tatcgcttgc ggcaaaatga ttttttgcat tgtccgatta taagaaagac ctaaacttct
    12481 ggaagcttcc atttgtccga caggaacggc gttgatcccg cctcgaacaa tctctgcgat
    12541 ataagcactt gcgttaagag tcaaggtgat aacaccagca aggaagtcag gaatcgtaaa
    12601 tccaacgata ccaggcaacc caaagaaaat aaagaacgca agaaccatca ttggaattcc
    12661 tcggatgata tcgacataaa tactagcaat tgtgcgaaga gcgcgtattg gtgctacact
    12721 gaataatcca aaaatcacac cgattactat agccaaagca aaggagatca aagttaggag
    12781 aattgttctc caaagtcctt tcaacaatgc ttgatagttg ttttggataa gtccaagaaa
    12841 tgttgattca tcagcagtgg aagactcact ggaagtttct gaaccatccg ctacatattc
    12901 tttaataatc ttatcgtatt cgcctgttct ttttaattct ttcaatcctt cattgaacat
    12961 attaagaagc tcaggatttt gtcctttttt aacggcaaat gcatattttc cgccagattc
    13021 ttttttgatc ggtgtttcca attcttgtcc ttgtttgatc gcataaccga ttaccggata
    13081 atcatccatc attgcatcga ttgcgccaac ttttaacccg tcgtacaatt gatcagcagc
    13141 atcaaaatat ttgatagaat agccatattc agattcatgg tcgcttaaaa attcagcact
    13201 ttcggtacca acttttgctc cgactgtttt tcctttcaaa tcttcataag acttgatcga
    13261 agtatctcct tttttgacag ctaattggat cccgctatca aagtaagaat cagaaaaatc
    13321 atagcttttc tttcgttcat ctgttacagt cattccggca atcattccgt ctgcttgtcc
    13381 tgattccaaa gcttgcaaag cagcagagaa accacggtag tccattcgga tgttaaatcc
    13441 ttgcagtttg gcgatgtttt ccatcaattt tacatcgatg ccttcgtact cgccctttgc
    13501 gttttgaaat tcaaatggag caaaagcaga gtcactggca attacgtata cgtctttttt
    13561 gggctctatt ttttgcattg tctcgcttgt gcttgactct gccgttgtag tgttgtcagc
    13621 ttgtgcaaca tgtccaaacg caagtgtcac aagtcctgtc attaagaaaa caaatgagtg
    13681 aaataaatgc tttcttttca taatcaacct cctaaatttc caatatttta ttattatagg
    13741 cgaaaagtgc tgaatcagac aagactgtaa gctcgaaaaa atgaaaaaaa tgcgagtttc
    13801 taacatttta tgttataaaa cgtaacatgc ataataagat aaaaaagtgg cgaaagtatg
    13861 ttcggtgtgg tataattttt tagtaaggaa tgcctttgct ttccttttct ttttattggt
    13921 cgtaaaatga aaaggaatga atgaaaagga ggacgaagaa tgattgagag agatacctcc
    13981 cgacactttg agcttgtctc gaaataccaa ccggctggtg atcagcctga agcgattaac
    14041 caattagtgg atggcgtagt cggcgggaaa aaagcacaaa tcttattagg tgcgactgga
    14101 acagggaaaa catatacgat ttccaatctt attgagaaag tgaataaacc aacactaatt
    14161 attgcccata acaaaacatt ggctggtcag ctgtacgggg aattcaagga atttttccct
    14221 aacaatgctg ttgaatattt tgtttcttat tatgattatt atcagcctga agcttatgtg
    14281 ccttcaagcg atacgtatat cgaaaaagat tcaagtgtaa atgatgagat cgacaaatta
    14341 cgacattcgg ctactagttc attattagaa agaaatgatg tgatcgtcat tgcatccgtt
    14401 tcatgtattt ttggattagg ttcgcctttt gagtaccaaa aacaagtagt ttcgattcga
    14461 cagggagcag aactagatcg caatcagttg attcgtgact tagtcagtat ccaattcgaa
    14521 agaaatgata tagattttca gcgcggtcgt tttcgagtgc gcggtgatgt cgtggagatt
    14581 tttccggctt caagagatga acgtgcctta cgtgtggagt tttttggaga tgaggtcgaa
    14641 cgtatccgag aagtcaatgc actgacaggt gaagttttag gagaaacgga acacgtggca
    14701 atcttcccgg cgactcactt tgtgacgaat gatgaacata tggagcatgc cgtagcgaat
    14761 atcaaagcgg aattagaaca gcgtttaact gtcttgcgaa atgaaaacaa acttctggaa
    14821 gctcaacgtt tagaacagcg aaccaattac gatattgaaa tgatgttaga aatgggctac
    14881 acgtcgggaa ttgaaaatta ttctcggcat atggacggac gtaaagaagg tgaaccgcct
    14941 tatacgctat tggatttctt tcctgaagat tttttgatcg tggcggacga atcgcacgta
    15001 accatgcctc aagtcagagg aatgtataat ggtgaccgag cgagaaaaca gatgctagta
    15061 gattacggtt tccgtttgcc atctgcttta gacaatcggc cacttcggtt agaagagttt
    15121 gaaaaacatg tcaatcagat catttatgtc tctgcgacgc caggaccata tgaacatgaa
    15181 caaacagata ccgttatcca acaaatcatc cgtccgacag ggctgcttga cccagtgatc
    15241 gaagttcgtc caatcatggg acaaatcgat gatctagtag gcgaaataaa tgaacgagtc
    15301 gaaaaagacc agcgggtgtt tgtaacgaca ttgacgaaaa aaatggcaga agacctcaca
    15361 gattatttca aagaattagg gatcaaagtc aaatatttgc atagtgatat caaaacgctg
    15421 gaacgaacag aaatcattcg agatcttcga ttaggtgaat ttgatgtgtt agttggaatc
    15481 aatttgcttc gtgaaggatt agacgtacct gaagtttccc ttgttgctat cttggatgcg
    15541 gataaagaag gctttttacg tagtgaacgt tccttagtcc aaacgattgg acgagctgct
    15601 cggaatgaag aaggtaaagt aatcatgtac gcagacaaag taacagattc tatgcgtcta
    15661 gcgatggatg aaacatcaag acgccgtacg attcagcaga agtacaatga agaacatggg
    15721 atcgttccaa aaacgatcat taaagaaatc cgagatttga tttctattac gaaagaatcg
    15781 gaagatgata caaaagaagc ggtacaagtc tcgtatgaag aaatgacgaa agaagaaaaa
    15841 gatacgttat tgatgaaatt agaaaaagaa atgaaaaatg cggcgaaagc attggatttt
    15901 gaaacagccg ctaatgtgcg agatatgatt ttagaattaa aagcttcaaa gtaggaggac
    15961 aacatggcta acgataaaat tacgattcac ggtgcacgtg cccataactt gaaaaatata
    16021 gatgtaacga ttccacgaga taaattcgtg gtcgttacag gtttgtccgg ttctggaaaa
    16081 agttccctag catttgatac gctgtacgct gaagggcagc ggagatacgt agagagcctt
    16141 tctgcttatg ctcgtcagtt tttaggacaa atggataagc cggacgtaga tagcatcgac
    16201 ggattgagtc cggcgatttc tattgatcag aaaacaacaa gtaaaaatcc tcggtcgaca
    16261 gtaggtactg tgacggaaat caatgactat ttacgcttgt tgtttgcacg tgtaggacat
    16321 ccgatttgtc ccaacgatca tatcgagatt accagtcaat cagtagatca aatggcagat
    16381 caagtaatgg ctttgcctga gcgcacaaag atccaagtgt tggcaccgat cgtggtaaag
    16441 aaaaaaggac aacataaaaa aatattcgaa cgaatccaaa aagagggata cgtccgcgtt
    16501 cgagtcgatg gtgagatcta tgatttaagt gaagtgcctg agctggagaa aaacaaaaaa
    16561 catgatatcg cgatcgtcat cgaccggatc attgtgaaag aagggattcg ttctcgtcta
    16621 tttgattctt tagaggcggc tctgcgattg gcagacggct atgctctggt tgacgtgatc
    16681 ggtgaagaag aaatgttgtt tagcgaacac tatgcctgtc cttattgcgg atttactgtt
    16741 ggcgaactag aaccaagact gttctcattc aatgcgcctt ttggagcttg tccagactgt
    16801 gatggtttag gtgtcaaatt agaagtcgat caagatttag tgattccaga tagaacgaag
    16861 acacttagtc aaggtgctat cgttccatgg aatccaatca gctctcagta ttatcctcaa
    16921 atgttagcgc aggcttgtga aagtttcggt atcgatttag atacaccatt cgaagaatta
    16981 ccggaagagc atcaggatat catcttgaat gggacagatg atcttttcca tttccattat
    17041 gaaaatgatt ttggtggcgt tcgcgatgta gaaacgactt tcgaaggggt attgaagaat
    17101 atcgagcgtc gttatcatga tacgaacagt gatttcacgc gtgagcaaat gcgtctttat
    17161 atgacagaat taacttgtca gacatgtaaa ggttatcgat tgaatcccca agctctatct
    17221 gttcaaatca atggaacgaa tatcgggcaa acaaatgagt tgtctattca aaaagcagta
    17281 gatttcttct caaacttaac gttatctgaa caagagaaag taattgccaa accgatttta
    17341 aaagaagtaa atgacagact ttcattcttg gaaaatgtgg gactagacta tctaactttg
    17401 agtcgcgctt ccggaacatt atctggggga gaagcgcagc gaatcagatt ggctacacaa
    17461 attggatcga atctttctgg agttttgtat attttagatg aaccatcgat cggattgcat
    17521 caacgcgaca acgatcgact gattggctca ttaaagaaaa tgcgtgatct tggcaacacc
    17581 ctcgttgtag tcgaacatga tgaagatacg atgtgggcag cagattatct gattgacgtc
    17641 ggacctgggg ccggagaaca aggcggacaa atcgtggctg caggcactcc tgaagaagta
    17701 gctaatgatg aaaattcttt gacagggaaa tatctttcag ggaaaaaatc aatccctgta
    17761 ccaaaagaac gccgtaaagg taatgggaaa gcaatcaaga tcactggtgc ttcagaaaac
    17821 aatctgaaaa atattagtgt tgaatttcca ttaggagaat ttgtagcagt cacaggtgtg
    17881 tctggttctg gaaagagtac attggtcaat agtatcttaa agaaatcgtt agcgcaaaaa
    17941 ttaaataaga attctgctaa gccaggtaaa ttcaagacaa tttccggcta cgaaagtatc
    18001 gaaaagatca tcgatatcga tcaaagccca atcggccgga cgccgagaag taatccagcg
    18061 acttatacaa gtgtatttga tgatatccgt gggttatttg ctcaaacgaa cgaggcaaaa
    18121 atgcggggtt ataagaaagg gcgttttagt ttcaacgtaa aaggcggtcg ttgtgaagct
    18181 tgtcgcgggg atggaattat taagatcgaa atgcactttt tgcctgatgt ctatgttcct
    18241 tgtgaagtat gtcatggcaa acgatataac tctgaaacat tagaagtgca ttacaaagga
    18301 aaaagcattg ctgatatttt ggaaatgaca gtagaagatg ctgtagaatt cttcaagcac
    18361 attccaaaga ttcatcgcaa actgcaaacg attgttgatg ttggcttagg ttatgtgacg
    18421 atggggcaac cagcaacgac attgtccggt ggtgaggcac aacggatgaa acttgccagt
    18481 gaattgcaca aaatctctaa tggaaagaat ttctatatac tagatgaacc aacgacagga
    18541 cttcatagcg atgacatcgc ccgcttgttg catgtattac aaagattagt agatgctggt
    18601 aacacagttt tagtgattga acacaatcta gatgtaatca aaacagcaga ttatatcatt
    18661 gatttaggac cagaaggtgg agaaggtgga ggaacgatcc ttgcgactgg aacaccagaa
    18721 gaaatcatta acgtaaaaga aagttataca ggtcactatt tgaaaaaaat aatggtataa
    18781 agaggataga tagctgagat agttaaaaaa acataaaaaa atgaattttt gcttgtttta
    18841 ttaatatcag tgttctagaa tcattacatg gtagttcttt ctacactgat tggtatagac
    18901 aaaaacttcc tataagttca atccgttcaa aaaagaattg agcaatcgga ggagtttcct
    18961 ttgattgctt ttttctttat ggtatgatat tccttgtgta gttggaaaag aaaaacaaga
    19021 gattatgagt aagtgagaga taatgatgaa cagtattgaa aatgaagaat ttatccctat
    19081 attattagga agcgacatga acgtttatgg tatggcacgt tcttttaatg aagcatacgg
    19141 caaaatctgc caagcatatg cttcggatca gttagcccct acccgctaca gtaaaattgt
    19201 caatgtagaa gttattccag gatttgataa agaccctgtg tttatcgaga cgatgcttcg
    19261 tttggcaaaa gaaagatatt ctgataagag taaaaaatat cttttgattg cttgtggtga
    19321 tggatatgca gaattgatct cccaacataa acaagagctg tcagaatatt ttatctgtcc
    19381 atatattgat tattctttat ttgaaagact gatcaataaa gtaagctttt atgaagtttg
    19441 tgaagaatat gatcttccgt atcctaaaac attgatcgtc agagaagaaa tgttagtaaa
    19501 tggacatttg gaacaggaat tgccttttga attcccagtt gctttgaaac ctgcaaacag
    19561 tgtggagtat ctttctgtcc aatttgaagg aagaaaaaaa gcttttattt tagagactag
    19621 agaagagttc gatttaattt taggccggat atacgaagcc ggttataaaa gtgaaatgat
    19681 tgtacaagat tttatcccgg gtgatgatag taatatgcgt gtgctaaatg cgtatgtaga
    19741 tgaagatcat caagttcgga tgatgtgtct tggtcatcca ctgttagaag atcctacacc
    19801 agcatcgatt ggtaattacg ttgtgatcat gccagattat aacgaaaaaa tctatcaaac
    19861 cattaaggca tttttggaaa aaatcgaata tacaggcttt gccaatttcg atatgaagta
    19921 tgatcctaga gatggagaat ataaattatt tgaaatcaat ttgagacaag gtcgtagcag
    19981 tttctttgtg acattgaacg gcttgaatct ggctcgtttc gtcacagaag atcgagtgtt
    20041 taacaaaccc tttgttgaaa cgacctatgg aacgaatcaa tcagataaag caagattatg
    20101 gatgggtgtg cccaaaaaaa tctttttaga atatgctcgg gaaaatgaag acaaaaaact
    20161 agctgagcaa atgatcaaag aaaatcgtta cggtactact gtgttttatg aaaaagaccg
    20221 gtcaatcaag cgctggttgt tgatgaaata tatgttccat aactatattc caagattcaa
    20281 aaagtatttt catgtgaaag aaggctagtt catggagaat tttttcagta ttttaggcgg
    20341 aatgggcacg atggcgacag aaagttttgt tcgcttgatc aaccaccgaa cgaaagctac
    20401 aaaagatcaa gaatatttga attatgtctt attcaatcat gcaacagttc ctgatcgtac
    20461 agcttatatt ttagatcgat cagaagaaaa tccaatgccg tttctgctgg atgatattga
    20521 gaaacaaaat ttattgcagc cgaacttcat tgtgttgact tgtaacaccg cacactactt
    20581 ttttgaagaa ttacaagcgg caacggatat tcctatcctt catatgccaa gagaagcagc
    20641 aaatgaactt gtccgtcagc atacgacagg gagagtagcg attctaggaa cagaaggaag
    20701 tatgaaagct ggaatctatg aacgcgaagt caaaaatctt ggattcgaaa cagtgattcc
    20761 tgatactgtt cttcaagaaa aaatcaatta tctgatctat catgagatca aggaatcgga
    20821 ttatttgaat caagagctat attacgaaat cttagaagaa gcggtagagc gcctaaactg
    20881 tgaaaaagtt atcttaggtt gcacagaatt gtcattgatg catgaatttg ctgaagataa
    20941 ccattatcca gtgatcgatg cccaatctat tttagcagat cggacgattg aacgagcgct
    21001 agctgaaaga agcaaggcat tggatacagc atcggaaaag taaaaaaatt ggatttgggg
    21061 caaatatcct aaaagatatt tggtccaaat ctttttgtaa aagaaaaaga aaactggtaa
    21121 tgatacgccc ggaatgatat aattttacta tgcacaatgt gagaggagtt cgtaattatg
    21181 acagataatt tgcaattagt catcataaca ggtatgagcg gtgcagggaa aacagtagct
    21241 atccaaagtt ttgaagatat gggctatttt tgtatcgata atatgccgcc tcgattgatt
    21301 ccaaaattct gggaattaat caaagaatca ggcaaagtca caaaaatggc tttaggagtg
    21361 gatttacgtt ctcgttcctt ctttgaagaa atccaaaaca tgctgatcga aatcgaaaat
    21421 acgaacttca tcgacacttc tatcttattt ttagatgctt cagacgaaga actagtggct
    21481 cgctacaaag aaacaagacg agcacatcca atggcgatgg acggtttgat cactgaaggg
    21541 atacgtaaag aacgggcgat actagacgac ttgaaagccc aagcttccgt gatcattgat
    21601 acgacgacat tgacaccacg acaattacgt gaaaaaatca atgagtcatt caaatcaaag
    21661 ggagaccaag gatttcggat cgaatttgta tcatttggtt ttaaatacgg gttgccgatc
    21721 gattcggata ttgtgatgga tgtgcgtttt ttgcccaacc ctcattatat cccggaacta
    21781 agaccattga ccggaaaaga tcagccagtc tatgattatg tgatgtcctt tgatgaaacg
    21841 caatcattct accataaatt tttggatctt ctggaaactg tattgcctgg atacattaaa
    21901 gaaggaaaaa gcagcttgac agttgcaatc ggctgtacag gcggtcaaca ccgttctgtg
    21961 gcattgactg aacgtttggc aaatgcttta gggaaaaact ataaagtcaa tgtgacacat
    22021 cgggacaagg ataaaaggaa agagacggtg aaccggtcgt gaaaatgaaa acctaccgga
    22081 taagaaaacc aaagatcgta gtggtcggcg gtggcactgg tctgccagtt atcctgaaaa
    22141 gtttgcgaaa tcaaggagca gatatcacag cagtagtgac tgttgcagat gatgggggaa
    22201 gcagcggtgc aatcagagaa tcgatcgcta tggctcctcc tggtgacttg agaaatgtct
    22261 tagtggcgtt atcggatatg ccacaattct atgaagatat tttccaatac cgtttcaaaa
    22321 aggaagatca gtttttagcc aatcatacga tcggtaatct gatcattgcc gcagtttccg
    22381 agatgcgggg aagcacttat gaggcgattc aattattagc aaaaatgatg catgtagatg
    22441 gacatgttta tccttcttca gaaactcctt tgaccttgca tgctgtcttt aaagacggaa
    22501 cgactgcagt aggagaatct aaaatcgcga tcgaccgtaa aaccattgac cgtgtgtatg
    22561 tctgcaacac aaatgataaa agtgaagcca aagcggctcg aaaagtcgtt tcagcaatta
    22621 tggacgccga catggtcgtt ttgggacccg gaagtttatt caccagtatc cttccaaatt
    22681 tagtgatacc agaaatcggc gaagcaatgc tcaagacaac tgctgaaacc gtctatatct
    22741 gtaatatcat gacccaaaaa ggggaaacgg aacatttcac agatgccgat catatacgtg
    22801 ttttacacaa gcatttgaat cagcctttta tcgatacagt attggtcaat acggaaaaag
    22861 tgcctgaaga ttatatggac ccagagatct atgatgagta tctagtacaa gtcgcacacg
    22921 acttcaacgg attgagaaat gaaggatgtc gagtgatttc caccgacttt ttggaattga
    22981 aagatggtgg tgtcttccac gatggagaca aagtagtaga agaattgttc cgtttagttt
    23041 tcggttcaaa atattaagca atttattgtt ggagaaagga ggagaatagg atgtcatttg
    23101 ctgcagatgt gaagaaagaa ctaactggtt tggaagttca tcgagaacat gcgaaagcgg
    23161 aattagctgc tttgctgcga atgaacggct ctttaggtat tgtgaaccag cagtttattt
    23221 tgaacgtcca aacagaaaat gctgcaattg ccagacgtat ctattctctt ttgaaagatc
    23281 attatcaggt acgaagtgag ctactcgttc gtaaaaaaat gaaattaaaa aagaacaatg
    23341 tctatatcgt ccggttgaag caaggcacaa aagatgtgtt gatggatctt gatatcatgg
    23401 atggtatgat gttcaatgga cacgtttcgg acgagattat gggaaattcg caaaaaatgc
    23461 gttcttactt acgtggtgct tttatggcct caggttcagt caataatccg gaaacgagcc
    23521 gttatcattt ggaaatattt tctatttatg aacagcataa ccaagacatt tgcgatatgt
    23581 taaattatta cggtctacat gctcgaacgc ttgaacggcg caatggctat atttcttatt
    23641 taaaaggagc agaaaaaatt gctgatttct taacattgat tggtgcaacc aactcaatgc
    23701 tccgatttga agatgttcga atcgtacggg atatgagaaa ttcggtcaat cgtctagtca
    23761 attgtgagac agcaaatctg aacaagacga ttgatgcggc ttctaaacaa atcgaaaaca
    23821 tcgaattgat cgaagcaaag gtcggtctac atgcccttcc tgaaaaactt caggaaatcg
    23881 cagaacttcg gcttcaacat ccagaggtca gcttgaaaga attaggggaa atgattccct
    23941 caggtgcgat atcaaaatcg ggaattaatc accgaattcg caaaataaat gaatttgcgg
    24001 aaaaactgcg tgaagaagca gtttaatcca aagaaaaaac tgatacatga agcaggagat
    24061 gcgaatgtat cagttttttt taaaagttat acttgctttt cttccgtcaa actataattc
    24121 catctttact ggagtaacat aatttggaaa ggcttctatt gtttttaata caatcgtata
    24181 aaaagcgggg agacaactga atgaagattt tagttttgta cataaatttt tgcttaatat
    24241 agaagtcctg atatagataa gatcaagtat ttatctataa ctggcctgat taattaaaaa
    24301 actggaagga actgctcgat catccttgag aactgctctt cttacttctg gtcaagattc
    24361 tcgtggttgg tggataagga tggttgataa gttcattgga ttagctagtc gaaaacaacc
    24421 aggtgtaatt aatgtagaag ttttaacaag gggatggagt gatttattca gtgataatca
    24481 taagttcata aatagtaatt gagatgtatg gttggaatac gttatacctg aacttgaact
    24541 accaagattt ggtttagaat ttgagaatcg atgtttataa agggagggca aaaatgcgtt
    24601 tttttacaac ttttactatc attatgattg cagtactttt tatttttcta gatatagcaa
    24661 agcgaaatac agcattttta ttatatcggg ttttacttcg agcaggtctc ataacattta
    24721 tatcgatcgt tggatttttt ctattcacag ttattgtatt tatttggaga actccagcac
    24781 caccattgcc agagattaca tatggagaat ttccttttag acttgagtat gaattgaatg
    24841 aagaattaca tgtgatagaa gacacgttaa tagttgaatt tgatggattt ggaatgaacg
    24901 aaggcatagg gagatatcgt agatggacaa gtcgtttagc cagcggagaa gatttagtgt
    24961 tgctgttaga agttagtgat aataagcaaa tattttattt tccaggacct gctaattatt
    25021 acatgggaga taggttgaat ggttataatc atacttttcc tagtgcttct ttcattgaaa
    25081 gggaaagagg cattactaga agggatattt tacatgacaa agaattatta gaacaatttg
    25141 gtccgcttga tcaaaataca ataaatgaag aagaattgtt aaatcaatat aatataaggc
    25201 tagtgaattg ggaaatatct gaaccgattg ttaacaattt tggtgattag aaaacaatta
    25261 cgataaatag aaacaagcca aatcatcagc aagtaaaagc tgatgatttg gcctttctta
    25321 tacgagtagt aggaattcat agcgaatatt attacgatca gtagatattt tgatattaaa
    25381 ggagtattac gaactagtat gtgagaatgt agaggtgatt tgcgaataat gagaaattta
    25441 tcaaatagaa acaaaatatt aatcattatt gttgttatag ctgtgtttca tctgggaacg
    25501 aatgcggttt taagtagaat aatattgggt cctaagccac caagacctgc gattacccga
    25561 ggggagtttg attttagatt agaatatgaa gtagatgggg aaaggattgt gatagaagat
    25621 acaattgttg cattatttga tggattttct gcagatgcag gtagcatggc atggtatcgc
    25681 acgtggcgtt tgcatttggc gagtgacaag agggcacaac aattattgtt acatgagtta
    25741 gatgatggtc gaaaaatata ttatcatttt ggaggagctg gattttatat ggggaagtta
    25801 gtagcagtgt aactgtaaca aggaaagatt tgccaggtct tggcattcga aactccatat
    25861 tttatatcga gcgtgggaga gatacatcta ttgggaacag gaaagggtta actcttgaag
    25921 agttatataa tgactttgga atccgtcttg ttagttggga acacaatcca ccaatagaaa
    25981 atcgctttga ataatcgaaa tctattaatc catttcatca gccagtgaat gttgtaatgg
    26041 aacaatttgg tttagtgttc aaaaccattg aaatactagg ctgtgatcag caaaaatgta
    26101 tctgaataaa aaagagagca actattaaat gagaggagtt aggagttgtt agctttatac
    26161 gaatcaaagt ggcaaataca gatgcgtatg catttgtgtt tgccatcttg cgtttggtag
    26221 acgagatagg tataagctaa agaaacaact tgtttaaaat aaacgaccta gctcaacaaa
    26281 cgaccatcta atcaactaac agcatacata aaagcaacta tttttgaagt agaattgaaa
    26341 tcgggataac ttaaaaagtg attgggatac tagtgtgaat caccgtacat aaatattact
    26401 cgttaaggga atttgagaaa ttttgtatga aaatttaaga attagttagt atgcgcaagt
    26461 caaaaatatg aataaattat ttggtggaga taatttgctc agcttgtcat aagaaatgag
    26521 ataatctggc agatgcaagc ttttggacac catttattcg gaataaggag gctgggacag
    26581 aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt tataggtgtt
    26641 tagagcgtga gacaaaagtg ttctttactt ttgtcctacg ctccttattt ttttattttg
    26701 tttggatcaa ttcttttaat tcctctattg aaatagattc atcaaagatt tctttaccca
    26761 agatgacagt cggaacaaat cgaatatgtg cttgctcagc ttcgttgatg acagcttggc
    26821 tcagctgttc gtctttttgt tctgtcagct ttagtttatc tactgcaaaa tccgctactt
    26881 cttggagaga aagatgtttc cactcgtctt gagtatcgaa tatttgtttg atctctttga
    26941 tcgctttttg accatcgctg attgtgagat accggtgcat cacgtttcct cgctgcaagc
    27001 tttctttttc tttatcgaac aatttgatca ctcgctgcaa ttgcccgctt tggactgctt
    27061 cttctaatag ctcataagat tcttcaaacc attgtcggca atacggacaa gataggttga
    27121 tgaattcgac cattttttta ggagcattcg atgaaccata aaggattcct ttttgtgtat
    27181 ttgttttcgt tgcatcaatc actgatatat ccatgttaaa acacctcttt cgcttatagt
    27241 ttatacatgt ctgacaaaaa aaggcaacga tttagactgt ttgtctaaaa attagatgac
    27301 caattcaaat agacatatgt gaaatatttt tataaataaa agaaaaaatg gaatgtttta
    27361 cgttataatg gaacagataa taagagagaa tatgaacaag gatgatgaat catgcgaaaa
    27421 atgaaaacaa tggatggcaa tacggcagcc gcatatattt cttatgcatt taccgagatt
    27481 gctgcaatat atccaataac acccagttcg accatggctg aattagtaga tcaatggtca
    27541 gcagcaggaa aagaaaatgt attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa
    27601 gcaggagcag ctggtgtggt ccatggatca ttgaaaactg gtgcactgac aactacatat
    27661 accgcttctc aagggctgct tttgatgata ccgaatatgt acaagattgc tggggaacta
    27721 ctgccaagtg ttttccatgt tgctagtcgg gcagtgacga caaatgcgtt gaatattttc
    27781 ggagatcacg gagacgtgat ggctgcaaga caaacaggat ttgctatgtt agctgaaagc
    27841 agtgtacaag aagtgatgga tttagcacct gttgcccatc ttgcttctat tgaaggagaa
    27901 gtaccttttt tgaatttctt tgatggattc cgtacgagtc acgagattca aaaaatcgaa
    27961 gttttggaat atgaagaatt agctcctctg atcaatcagg agaaattagc ttcatttcgt
    28021 aaacgaggga tgaatcccaa tcatccgact gttagtggta cgaaccagaa tccagacatc
    28081 cattttcaac aacgggaaac gatcaacccc tattatgata aattacctga aatcgttcaa
    28141 aaatatatga aagagatcaa ccgattacga ggaacttctt atgacttggt gacttattac
    28201 ggagcagacg atgcggaaga agtcattgtt tctatgggat cagttgcaca gacgattgaa
    28261 caaacgattg attacttgaa tgcaaacgga cggaaaacag gttttttgaa tatccatctt
    28321 tatcgtcctt ttccagtgga aacattcgtg caaaagttgc ctcatacagt aaaaacaatt
    28381 gctgttcttg atcgaagtaa agaaccaggg gcaggtggag aacctctctt attagacgtt
    28441 caaagtgcgt tatatgattc tgaacttcgc ccggctgtta ttggtggtcg ctacggattg
    28501 gggtctaaag atgtgactcc cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac
    28561 ccaagcatac gcaaaaaacg gtttaccata gggatcgtgg atgatgtgac ttatcaatct
    28621 ttggaaccaa aggaaagtct ggatttgaca gaaccgcaaa cattccaggc caagttttgg
    28681 ggatttggat ctgacggaac agttggagca aataaatcag ctatcaagat tattggagat
    28741 cacaccgata aatatgcgca aggctatttt tattatgatt cgaaaaaatc tggcggtctg
    28801 actgtctctc atcttcgttt tggagatacg ccgatacgtt cagcgtatct tgtcgagcat
    28861 gcggaccttg ttgcctgtca cacaccagct tacctgcata gctatgactt ggtcaaagga
    28921 ttgaagccag gtggcatttt tttattgaat actctttgga gcgacgaaca attagaaacg
    28981 catctgccct taaaattaaa acgttacttg gctgaaaata acattagatt ctatacgatc
    29041 aatgccatga gattggccca agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa
    29101 acggcttttt ttaaattagc tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa
    29161 gaagaagccc tgaaaagcta tggacataaa tcaatggcta tcgtagaaaa aaacatacaa
    29221 gcaatcgatc ggacgaccga actgcttcat caagtcgcga tcccagacca ttggagaacg
    29281 ttggagatgc ctgaaaaaat caataaaagg aacactggtt atatacaaga aatacttgaa
    29341 ccagtgaatg cacaggaagg aaatcaactc tctgttggta cacttgtagc aaatggaatg
    29401 acaactggcg aaatgccttt aggaacggct tctatggaaa agcggggcgt cgctttagaa
    29461 gtacctgagt ggattagtga tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct
    29521 catgctgcca tccgtccatt tttagcagat gaagaggaaa tggaagaagc gccagaagga
    29581 tttatcgtga gagaaatgcg aggcgcagac ggcgtaaaat accgtatcca agtttcagtg
    29641 gaagactgta caggttgtgg tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta
    29701 gtgatgaagc cttatgaaga acagaaagaa caagctgtga attgggcttt tgcaatgaca
    29761 ttaagacaaa aagcaaatcc agtcaaagga aagaatactg tcttgtctac acaattcaat
    29821 caaccgcttc ttgaattctc gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa
    29881 ttattgacac aaatgttcgg tgatcgaatg atgatcgcga atgcgacagg ctgttcttct
    29941 atttggggcg gtgcttctcc tgcttctcct tatacgacca atgaatgtgg tcaagggcct
    30001 gcatggtcaa attctttatt ggaagataat gctgaatttg gttatggtat gcttctagcg
    30061 gcacagactc gtcgcgaggc tttggcaagt tcaatcaaag aagtattgcc aaaagtatca
    30121 gaagaactac gagaattgat ggaagactgg ttggtacaca tgtttgagag cgaaggaacg
    30181 cagcaaagag cagcaaaact gcaagctgcg atcgaaagcg aacgatcaat tgtccctgaa
    30241 ctggaggata tatacaagag aaaggatcta ttcgtaaaaa acagccaatg gatgattggt
    30301 ggagacggat gggcttacga cattggtttt ggcgggatcg atcatgtact tgccagcgga
    30361 gcagacgtga atattcttgt tttggataac gaagtctatt ccaatactgg cggtcaaacc
    30421 tcaaaagcaa caccagcttc ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca
    30481 aagaaagatc taggaatgat ggcgatgaca tatggaaatg tttatgtggc gcaaattgct
    30541 tcaggtgcca atcaaatgca gacgataaag gcttttgaag aagcagaaaa gtatcctgga
    30601 ccttctttaa tcattgccta tacgccttgt atcacacatg gtttgcttgg tggaatgaaa
    30661 gaatcgctta aagaagcaaa agaagctgtt tcttctggct attggtcatt atatcgatat
    30721 aaccctgcaa tggtcgaaaa aaacaagaac ccaatgactt tggactacaa aaaacctgat
    30781 ttctctgcta tgcctgattt catgaggaaa caagttcgct tttcctcgct agagaacgca
    30841 aatccaaatc ttgcaaaagc tttatttgaa aagactatcc aagacgctaa aacacgtttt
    30901 tacaactatg ctgcactgac gggagaatta gaaaaaatca agaaacgact tgaaccagag
    30961 gaatctatcg atgtacccgt taagactaga aaagaaaaaa cgacggacct tgaagcagaa
    31021 gaaagaagag ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa
    31081 aaggaaaaat aacagaaaaa atataatgaa taatt
//
LOCUS       DACIFS010000033        30423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.033, whole genome shotgun sequence.
ACCESSION   DACIFS010000033 DACIFS010000000
VERSION     DACIFS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.033"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(60..188)
                     /locus_tag="IYA83_001512"
     CDS             complement(60..188)
                     /locus_tag="IYA83_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ8586947.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="IYA83_001513"
     CDS             complement(191..1564)
                     /gene="rlmD"
                     /locus_tag="IYA83_001513"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ8586948.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1675..2730)
                     /locus_tag="IYA83_001514"
     CDS             complement(1675..2730)
                     /locus_tag="IYA83_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ8586949.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="IYA83_001515"
     CDS             complement(2740..4170)
                     /gene="gatB"
                     /locus_tag="IYA83_001515"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ8586950.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="IYA83_001516"
     CDS             complement(4167..5639)
                     /gene="gatA"
                     /locus_tag="IYA83_001516"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ8586951.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="IYA83_001517"
     CDS             complement(5639..5944)
                     /gene="gatC"
                     /locus_tag="IYA83_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ8586952.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="IYA83_001518"
     CDS             complement(6050..8086)
                     /gene="ligA"
                     /locus_tag="IYA83_001518"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ8586953.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="IYA83_001519"
     CDS             complement(8103..10337)
                     /gene="pcrA"
                     /locus_tag="IYA83_001519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ8586954.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10492..12051
                     /locus_tag="IYA83_001520"
     CDS             10492..12051
                     /locus_tag="IYA83_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ8586955.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12162..14093)
                     /locus_tag="IYA83_001521"
     CDS             complement(12162..14093)
                     /locus_tag="IYA83_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ8586956.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="IYA83_001522"
     CDS             complement(14111..15025)
                     /gene="pfkB"
                     /locus_tag="IYA83_001522"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ8586957.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15022..15774)
                     /locus_tag="IYA83_001523"
     CDS             complement(15022..15774)
                     /locus_tag="IYA83_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8586958.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15933..16142)
                     /locus_tag="IYA83_001524"
     CDS             complement(15933..16142)
                     /locus_tag="IYA83_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586959.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16153..17481)
                     /locus_tag="IYA83_001525"
     CDS             complement(16153..17481)
                     /locus_tag="IYA83_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586960.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17846..18433)
                     /locus_tag="IYA83_001526"
     CDS             complement(17846..18433)
                     /locus_tag="IYA83_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ8586961.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IYA83_001527"
     CDS             complement(18516..19766)
                     /gene="clpX"
                     /locus_tag="IYA83_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ8586962.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20013..21302)
                     /locus_tag="IYA83_001528"
     CDS             complement(20013..21302)
                     /locus_tag="IYA83_001528"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ8586963.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21456..22388)
                     /locus_tag="IYA83_001529"
     CDS             complement(21456..22388)
                     /locus_tag="IYA83_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586964.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22545..23204)
                     /locus_tag="IYA83_001530"
     CDS             complement(22545..23204)
                     /locus_tag="IYA83_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ8586965.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="IYA83_001531"
     CDS             complement(23207..24232)
                     /gene="sppA"
                     /locus_tag="IYA83_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ8586966.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24245..24535)
                     /locus_tag="IYA83_001532"
     CDS             complement(24245..24535)
                     /locus_tag="IYA83_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586967.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24945..26750
                     /gene="glmS"
                     /locus_tag="IYA83_001533"
     CDS             24945..26750
                     /gene="glmS"
                     /locus_tag="IYA83_001533"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ8586968.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26822..28177)
                     /locus_tag="IYA83_001534"
     CDS             complement(26822..28177)
                     /locus_tag="IYA83_001534"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ8586969.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28201..29370)
                     /locus_tag="IYA83_001535"
     CDS             complement(28201..29370)
                     /locus_tag="IYA83_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8586970.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29367..30254)
                     /locus_tag="IYA83_001536"
     CDS             complement(29367..30254)
                     /locus_tag="IYA83_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ8586971.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         8187 a         6590 c         5440 g        10206 t
ORIGIN      
        1 ctaaaaagac taagaaacaa taacaaaagt taacacttct tagtctttct ttagcacgtc
       61 tatttattaa agattttagg gccttttctc atgtttttac gatcattctt aggtgtttcc
      121 atttgtttgg atcggcctcc accttgtttc tttttgatca attctttcat tttttctttt
      181 tggtccattt ttattctcct ttaacgaatg aagcgactaa ttcgatatga tgggtttgcg
      241 ggaaaagatc tacaggctga actttcttca cggcataacc tgcttctgca aataacttag
      301 catctcttgc aaaagttgcc ggattacatg agatatagac gattcgttct ggtgacattt
      361 cgattgctga ttcaataaaa tgatgatcta atccttttcg tggagggtca actacgatca
      421 cagatggttt gatccctttt tcttgccatt ttttcataac tttttctgct tttccgactt
      481 cataatgaac attttcgatt ttattattta aagcattgaa ctgtgcatct tcaattgctt
      541 ctggaataac ttccatccca tacaccttat ccactctgtc tgctaaagac aagccaatcg
      601 tacctattcc tgaataagcg tcgatcacaa tgtctttttt atctaactcg gcaaactcga
      661 tggctgtttg gtacagatac tctgcttgtt gagtatttac ttgataaaat gactttgctg
      721 agattcgata ggtttttcct aatagctgat cttcgatata gtctctgcca tataaaactt
      781 tttctttgtc tcctaaaatc acatttgttt tttcttcatt gatattttgg atgaccgaaa
      841 ccacttctgg caattcttca tgaatagctt cagcaatttc cttggattta aagaattttt
      901 ctttacgagt aactaaaacg accatcattt catgggaata atgaccacga cggataatga
      961 tgtgacgaag ctgtccttca tttttttctt cattatatcc tcgtacttgg aaccgttgca
     1021 agatatctct gactactttg attgcttgat cgatgacagg atcttggata taaaaatctt
     1081 cgatagggac tagatcatgg ctattctttt tgtaaaaacc ggtggctaac tgtccatcga
     1141 tacggcgaac agggatctgt gctttattac ggtagccgaa tggttctgcc attccgaccg
     1201 ttggaagtac aggaacttct ggcatcttag caatcttcgc cattacatgc tctacttgtt
     1261 tttgtttgaa gacaagctgt tgttcatact tcaaatgggc taaaggtgcg atacctatgc
     1321 gaagtaatgc attgttttct acaggctgac gatcaggtga ttgtgtaata aatgtttcga
     1381 cttttccata tccaaactta ttgcctgctt ttactactag tatttctact tcttctccag
     1441 ggagtgcatt ttcgataaat aatggataac catcgatttt tgctaccccc attccttcat
     1501 aactcaagtc gacgatttcg actgtatatc gttcgttttt ctttactatt tgctgttctg
     1561 tcatattttg ctcctttaag cctatttttc aatcctactg aactctacta taaatcagct
     1621 aattacacaa ctaaaaccgg cactgacctt actacgttca gcaccggctt ttgattaatt
     1681 cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg tcgatatctt catccgttaa
     1741 acgttcgact tcttttacga attctttgct gacaacctct aattcttctt catctgtacc
     1801 tgtgattgct tcatcaggaa tctgatcggt attggcaaag aactcaatgt gttgatgaag
     1861 attttggaaa tgcatcggtg cttccccgcc gtattcacca tctaagttga tcattactgg
     1921 tcgattgata tcatcttctg tttcaacgaa caaactgctt gttttggtat agattattct
     1981 tggatcatcg acatgtcgac cgccattcag catgagtgct gcaatatgca agatctcaaa
     2041 gatatttgct gtcttcacaa tgattaaaga aaacttccca tcatccaatt tggcatctgg
     2101 cgcgatctgt tcgaatcctc caacagagtt cgtcagccct aggaaaaaca tcgaagcatt
     2161 tccgatatat tccccttcat catacttcaa gcgcattttg attggcttta ctcgagggag
     2221 catctctgct cctttcgcaa gataagcaag ataaccaaaa atactcttta gttctgatgg
     2281 aacttcataa gtcaattctg tcaaatagcc acctgcagcg atattgatga agtaactgtt
     2341 atcagaaagg ccgatgtcca tttttacggt ttggtgtttt tttatcactt ctgctgctgc
     2401 tttaatatta tctctaggaa tttttaatgc tcgagcatag tcgttcgttg taccagctgg
     2461 gatgatggcc attttaggac ggtgttcaag cggagcgatt ccattaacca cttcgttgat
     2521 tgtgccatcg ccaccagctg ccactactag atcaaaacct agttctgcga ctctacatgc
     2581 ttcattcttg gctgaatctg gttctggtgt agttgcatag gcgctggctt caaatcctgc
     2641 ttcttctaat gcctgtagga tatccgccaa attttttttc aataattctt ttcccgaggt
     2701 ggggttataa attacgcgtg cttttttcat tttcagcctc tatcgtttcg ctaattcatc
     2761 tttaagtagc ttattcacga caccaggatt tgcttgtcct ttagttgctt tcatgatttg
     2821 accgactagg aagccaaccg cacgatcttt tccatttttg aaatcatcga cagattgttg
     2881 gttgttatcc aaaatttcat taatgattgg cagtagtttg ttcggatcag ataattgcac
     2941 ccaaccattt ttttcaacta cttcttgggc ttctccgcca tttgtgatca attcacggaa
     3001 gacttttttg gcgatttttg agctgatcgt tccatcggcg atcaaacgaa tcattcctgc
     3061 taagttctca ggtgtcaatt ttgtctcagc taattccagt ttctcactgt tcaagtaagc
     3121 agaaacttca cccatcaacc agttagatgc ttgcttggca tctgcaccat tttccaaaac
     3181 agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat accattgcgt catactctgg
     3241 aagtccaaac tcacggatat agcgttcgcg acgagaagct ggcatctcag gcaaactagc
     3301 ttttactttt tgaatccatt cgtcatcgat cacgaatttc gggatatcag gctctgggaa
     3361 gtaacgataa tcactagacc cttctttgac acgcatcaaa actgttttat tcgttgtttc
     3421 gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa agaagaactt ttgcttgacg
     3481 tttttcttca aaagctaaac cttttttcac gaaagataaa gagttcaagt ttttcaattc
     3541 tgctttcgta ccgaactctt cttgtccata agggcgaaga gaaatattgg catcacagcg
     3601 catagagcct tcttccattt tcacatcgct gacttctgta aattggataa ttgatcgcaa
     3661 tgcttctaga taagcgtatg cttcttctgg tgaacgcata tcagcttcag aaacgatttc
     3721 gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa taaccgccaa taccatgcat
     3781 gttttttcca gcatcttctt ctaaatggac acgttcgatc cggatttttt tctttttacc
     3841 ttctacttcg atttcgatcc atccgtcatg accgattggt tgatcaaatt gggaaatttg
     3901 atatgctttt ggattatctg ggtaaaagta atttttccgg tcaaaatgag tatctttaga
     3961 gatttcacag tttaatgcca aagctgcttt catcccgaat tccagcgcag ctttattcat
     4021 tactggcagc acgcctggat aaccccagtc gatcacgttt gtattgctgt ttggttctgc
     4081 accaaaatga gctggcgctg gagagaaaat ttttgagttg gtttttaact cgacgtggac
     4141 ttctagtcca atgactgttt caaaattcat tcgtctttcc ctcctaaaat agctggttgt
     4201 tgtttatgga attctgttgc ttgttcaaac gcatatgccg cttgatacat cgttttttca
     4261 tcaaaataat taccgatgat ctgcaatcca actggtaatc cttctgataa acctgccggt
     4321 acagacatac ctggaagtcc agccaagttg acagggatcg ttaagatgtc actcatgtac
     4381 attgtgattg gatcgttaat gttctcaccg attccgaatg caactgttgg tgaagatggc
     4441 ccgatgatca agtcataatc tgcaaagact ttttcaaaat ctcgtttgat caatgtacgg
     4501 acttgtcccg cttttttgaa gtaagcgtca taataaccag cacttaagga gaatgtacct
     4561 aacatgatac ggcgttttac ttcttcaccg aatccttctg aacgtgagtt gacatataca
     4621 tcatctaatg atttcacatt ttctgagcga taaccgtagc ggataccatc gaatcgttgc
     4681 aagttggaac ttgcttctga tgaggcaatg atataataaa cagccacacc gtattttgaa
     4741 tgaggcaagc tgacttcttc cacaacagca cctaatgaac ggaatgtttc tgctgctttc
     4801 aatacagctt cttttacgcc aggggccaca ccttcgccta agtattcttt aggtaaagca
     4861 atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa aatcaggaac tgaaacgcct
     4921 gaagaagtac cgtctttttc gtcataaccg ctgatagctg tcaatgctaa tgcattatct
     4981 ttcaccgtac gggtcattgg tccgatttgg tcaagagatg aagcaaaggc aatcaaacca
     5041 aaacgtgaga cacggccata agtaggtttc atccctacga tgccgttaaa agctgctggt
     5101 tggcggatac ttcccccagt atcactacct aaagaaactg gaatttgtcc agctgcaact
     5161 gctgctgcag aaccaccaga agagccacca ggtacttttg ttgggtccca agcatttttt
     5221 gttttcttga aataagaagt ttctgtactt ccacccatag caaattcatc catgtttagc
     5281 ttaccaacag ggatcatatc tgcttgatag attttatcca tgaccgttgc atcataaatt
     5341 ggttcaaaat tgtaaaggat cttagatgca gcggttgtca agatatcttt tgttacgata
     5401 ttatccttga taccaattgg aataccagca aggacgttgc tttcatcaat ccctttttca
     5461 tctaaagctt ttgcctgttc caatgctttt tcttcgttca atgtaatgaa tgcttcgacc
     5521 tcaggctctg tttctttgat tcgttcaaat gttgcttttg tcaaatcttg tacagtgatt
     5581 tctttcgata caagcatatg atgcagttct tctagagaac gattgtgtaa ttctgtcatt
     5641 atgcgccagc ctccccatta tcgatgattg cgggtacttt gataaagccg tcttctgttt
     5701 ccggcacatt tttcaataat tcatcgcggt tccatccagc ttgcgcccga tcttgtcgca
     5761 tcacgtttac tgtttcaacc acattcgacg taaatggaac accttctgta tctacttctt
     5821 ccagcatttc aaccatatcg atgattttac ctaattggtc ggtaaaactt gcaagttctt
     5881 catctgcgaa cgcaagtttt gctaatttgg caacgtgttt cacttgttct tcactaatcg
     5941 ccatactacc ccttctttct aaacgagtta aaaactttgc tctctatttt aacacaagag
     6001 tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa actcattttt cattcttcta
     6061 ctgagcgact tttagccaat gcatctgcca tttgatcttc tgtccaaact tcgattccta
     6121 attcttgtgc tttggtcagc ttgctgccag catcttctcc tgctaccacg atatccgttt
     6181 ttttcgatac gctaccagtc actttcccgc caagattttc gatcgtctct tttgcttctt
     6241 cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt tttttcttta aaaggagact
     6301 cgacttcttg caattgggca tttcgaagcc ctttgtattc gaaattgacg cctgcttgtt
     6361 ttaattcatt catcaattca tggacttctt cgttactgaa ataagtgact acactgtcgg
     6421 caatgatatc accgatcgta tcaagcgcaa taatcgactc atagtcactt ttactcaatg
     6481 tttctagatc accgaaatgt tctgctaaaa tttttgctgc tttcgctccg acatgtcgga
     6541 ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt gctgttgtcg attgctgtca
     6601 aaatattatt tgctgatttt tcttttattt tgtccaacgt caataactct tcttctgtca
     6661 atttgtaaag atcagcaaca tctgctacta gttttttatc atacatctgt tctagtacgc
     6721 gcggccccaa cccatcgata ttcatcgcat ttcttgaaac aaagtggttc aaaccttctt
     6781 tcatttgagc agggcattta ggattgatgc accgtaaggc tacttcttca tccaaatgga
     6841 caagttcact tccacaaact ggacaatgtg taggcaattg gtattcttcg ctgtctttag
     6901 gtcgtttatc taacacgact tgcgaaactt ctgggatgat gtctcccgct ttataaatca
     6961 gaactgtatc tttcaagcgg atatccttca acttaatata atcaccgtta tgcaggctgg
     7021 cacggcttac tgtcgttcct gctactcgga ctggtgtcat gatagctgtt ggcgttacta
     7081 cccctgttcg accgactgtc cattcgatgt tttcgattag cgtttcaact tcttctggcg
     7141 gaaacttgta agctgctgcc catctcggtg ctttgacagt aaaaccaagt tgatcttgta
     7201 gcgagaattc attcactttg atgacgattc catcgatttc atagggcaaa tccacacgtt
     7261 tgtcatgata ttcttcaatg tatgaccaca cttcatcaat cgaatgacat agacgttttt
     7321 cccgattcgt gtggaatcca attttttcta gctcttctag cgcatcatac tgggtttttg
     7381 cttgcatagg accgaaatca gctactgtgt ataaaaaagt gtccagattt cttttcgctg
     7441 tgattttaga atctaactga cgcaaagaac cagctgcggc gttccgagga ttcgcaaaga
     7501 tatcttttcc ctctgcttct ctgtcttggt tcaattggac aaatgaccgt tttggcatga
     7561 aacattcgcc tcttacttcg atgttcattg gttcttttaa tttgattggg accgaccgga
     7621 cagtttttag gttttcagtg atgttttctc ctacagttcc atccccgcga gtcgcacctc
     7681 tcacgaaatt tccatcttca taacgcaaag aaacggaaag tccatctatt tttagttcac
     7741 agcaatagtc aactacacgt cccactgctt tttgtacacg ttgatcaaaa gctatcagct
     7801 cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat atcatgagtg actttttcaa
     7861 aaccttctaa tacttttccg cctactcgtt gagtgggaga atcagacgtg atcaaatcag
     7921 gatattgttc ctcaattgct actaattctg cgtattcttt atcataaaca tagtcttcca
     7981 ctgttggttt atcttctacg tagtattcgc gagaccattg gttcagtctt gtacgaagtt
     8041 cttctgctcg tttagcagct tcgtcaatcg tcatttcatg ttccatcatt taccctccac
     8101 tactaagctt tttcaattgg cgcaaaggca gccaaaagac gttttactcc ttgacttggg
     8161 aaagccacat ctagttctag atccttcgac gtcccgctta ctcggacgac tgttcctaga
     8221 ccccattttt tgtgtttgac tttatctcca ggtttccagc tgttattttc ccctccagta
     8281 gcacttttag agggaacaac cgctgtctct ggctgtttat agctatatgc tgttttttta
     8341 gcgaatacag gtgttttttt aggcgtagga cgcatgcctt cgatttccag taactcctga
     8401 tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg tccgtccata taatgttctg
     8461 gagaaagcat ttgttaaata caacgcttct tctgctcgcg tgatccccac atatgcgaga
     8521 cggcgttctt cttccagttc gctttcttcc atcaatgctc gagataaagg aaaaaccccc
     8581 tcttctagac caatcaggaa tacaactggg aattctaatc ctttggctgc atgcaaagtc
     8641 atcaatgtca cttgggaagc atcttcttct aaattgtcaa tatcggaaac aagtgccaga
     8701 tcatttaaga agaccgttag tttttcttct ggtgcatcag cgtcttcttc attttgctgt
     8761 tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt cctccaagtt ttcgatgcgt
     8821 gcttgtgctt ctaatgtatt ttggattttt aagtcttcta gatatccgct acgatccaaa
     8881 acctctttcg tcaattctgt cactgtcaag tatgggatca tctgagtgac ttcttggatc
     8941 atctctccaa atgcacctaa ttgctgtcct gcttttccac cgatatttgc cagatccaca
     9001 ttttgcgctg cttcaagcag tggccattca tgaagagaag caaaactgcg tagtttttcg
     9061 atagatcctg gtccgatgcc tcgtttgggc gaattaacga tacgctcaaa actgattgaa
     9121 tcttgaggat ttgccagcac attcaaatac gctaaaatat ctttgatttc tttacgatcg
     9181 tagaacttgt gccctccaac cattttataa ggaatatttg ctttcaacag agtttcttcc
     9241 attacacgag attgtgcgtt cgtccggtac aagatggcaa agtcgccgta attccgatga
     9301 ttgcttcgaa tctcttcttg catccgatct acgataaaac gggtttcgtc tcgttcatta
     9361 tctgcacggt agtaagtgat cttgtttcct tctttgtttt ctgtccacaa gttttttggt
     9421 tttcgattgc tattgttttc aatgacttgg tttgctgcgc taaggatgtt tttcgtggag
     9481 cgatagtttt gttctagcaa aattactgca gcatctggat aatctttttc aaaatccaag
     9541 atgttctgca tatctgcacc gcgccagccg taaatacttt gatctgcatc tcccacgacg
     9601 cacaggttcc taaatctacc tgccaataaa ttgactagcg tatactgtgc gtgatttgta
     9661 tcttggtact catctacatg aatataatgg aatttgtttt gataatatgt gagtgaatca
     9721 ggatgttctt caaataatcg aatcgtattc atgatcaggt cgtcaaagtc catacattga
     9781 ttatattgaa gctctttttg gtaagcagca taacattttg ccgcaatctc ttcgtaataa
     9841 ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc tgttttttgc ttgagagatt
     9901 gttcctaata tacttcgagg atcatatttt ttcggatcaa tgttcagttc ttttagtatc
     9961 cgtttcatca aggtcagctg ttcagaagaa tcgatgattg taaagttccg gttatacccg
    10021 ataaaatcaa catctcttct taaaatacgc acacacatcg agtgaaaagt cgatacccag
    10081 acatcttcgc ctccagatgc gagaatcgca ttgacacgtt ctttcatttc ttttgcagcc
    10141 ttattggtaa aagtaatcgc taggatgttc caaggattga cttctttttc ctcaatcaaa
    10201 taggcaatgc ggtgtgtcag cactctcgtc tttccacttc ctgcacctgc cattagcagt
    10261 aatggtccgt cagtgtgaag aacagcttct ttctgacggg ggttcatccc gtttaataat
    10321 tcagctttgg atgacattaa caacatcctt tcttttatca atcttgttaa ttatagcatt
    10381 ttcttggctt tcattaaact agctagttca aaatcatttg tttcttttta tggatttttc
    10441 cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc gaggcgtttg aatggaaaaa
    10501 aaaggaaaaa tcaagaaatt agataagact cagatatatt ttattctaaa aggaatattt
    10561 gttggactgc tgacaggaat cgtcgttagc ctgtttcgat tatctatcga aaagctaagc
    10621 gatttagttc gaagtatcta tgaaatgagt cgggaacagc cgatttactt aattggccta
    10681 gctatttgct gcatccttgc agcatttttc gtgggttatc tggtcaaaaa tgaaccggat
    10741 atcaagggaa gcgggattcc acaagtagag ggacagcttc gcggggaatt atcaatgaac
    10801 tggttctctg ttttatggaa aaagttcatt ggcggtgtac tttcagttgg tgcgggcctt
    10861 tttctaggta gagaaggacc ttctatacag cttggcgcat ccattggaca aggagctgga
    10921 caattgtttc ggagtccatc ttcagaagaa aagattttaa tttctagcgg tgcaagtagc
    10981 ggacttgcag cagcattcaa tgctcctatc gcaggattac tctttgtttt agaagaaatc
    11041 catcactcct tttctccact tgtttggctg acatcatttg cttcagcaat cactgctaac
    11101 ttcgtctctc tttacttttt cggcttgaga cctgttttat atctaggtga ccttccttct
    11161 ttgccgttga aatattatgg ttccttagtg atcttaggag tacttctagg catcttaggt
    11221 tttatttatc agaaggtctt gctggctttg ccaaaatttt acaaccgatt gcctttacca
    11281 gcttattttc acgggttcgt tcctttcttg ctgattttgc caattggtta tttatggcct
    11341 agtactctag gcggaggcaa tcaaatcgtt ttagcttttg gacatgcttc cttgccaatc
    11401 attatgatta tcggtttgtt tgttcttcga ttcgtttttt ccatgatttc ttacggtggt
    11461 ggtcttccag gggggatttt ccttccgata ttgacattgg gggctttaat cggtagcttt
    11521 tatggaaaca ttatggttga tttcgggatg gatccaatct acatcaagga ttttgtcgtt
    11581 attgccatgg ctggttattt cacagcgatt ggaaaagcac cgctgacagc aattatttta
    11641 gtgacagaaa tggttggctc tcttaatcat ctgatgcctt taggattggc tgctcttgtt
    11701 gcttatatcg tcaatgacct tctcggcggt aatccaattt acgaatcatt attggaacgt
    11761 ctacttggaa atactcagac cgcctcacta aaaggtgcaa ggacaacttt tgattttcca
    11821 gtaacggcag agagttcttt agacggatta atggtacgag acttcaattg gcctaaaaat
    11881 atgctgctaa tctctatacg cagaggaaat caggaaatct tgactcacgg tgatacgatc
    11941 atgaatgtag gagatatttt ggttatcttg actgatgaag caaacttgcc aagaatcaag
    12001 caggaaatcg aggctaaatc ttctatcaca aatttgaagc aatcatttta acgaacatgg
    12061 aaaaactatt ttacaacaac tattcatcta cactacttga ttctatatga ttctataaaa
    12121 aaagaagaga ctgggaaaaa ccagcctctt cttttttatt tctatgcttc tgctgctgca
    12181 ggagcttttc tcaataaacc ataaatcaca ccagatacga ctgctccgat agcaataaac
    12241 accaaataca tgaccgggtg actcagtaag aatacaacga aaatcccgcc atgaggagcc
    12301 agtaatttga tcccgaaagc tccgactaat ccgccagtta aagcagaacc aataataaaa
    12361 cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa tcgatccttc cgtgacaaac
    12421 gatagtccca tcacgatgtt cgtcaaacca gcatcttgat ctgttttgct gaatttattc
    12481 ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta ccattcctcc agccataaca
    12541 gaagccatca cgacacttcc gccagttgca acagtcgtag ctaaagttgc tgtaccaaac
    12601 acataagcgg ctttgttgat tggaccgcct aaatctgccg ccatcattcc agctaataat
    12661 gcaccaagta aagcagcatt cgaaccactc atacttgtca agaaaccgtt caatgcatca
    12721 ttgattgctt tcatagggac attgaccagc aacatcaaga aaccagtaat cagtaagcca
    12781 aacactggat aaaagaggat cgttttaatt ccatccaatg attttggtaa gttcttgaat
    12841 aatttacgta aaaatacgat gacataacca gctaagaatc caccaatcag tgcacctaag
    12901 aatcctgcac cgcctgtatt agctaatgca ccagcagcaa atccaacgat caaaccaggg
    12961 cggtcaccga tacttgaagc aataaatcct gctaataccg gcagcatgaa accaaatgca
    13021 gcttgtccga tttggttgaa ccagctagca gcttgattgt aattccctag atttgataat
    13081 tgatcatgtg gcacgccaat aaattggtcg atcatgaaag aaagcgcaat agcgattcct
    13141 ccaccaataa cgaaaggtaa catgtgtgat acgccgttca tcaaatcttt gtaaattctt
    13201 tgtccgatag taccatccgc atttccttct tctgaactgc tttcttttcc atcaccatgg
    13261 aatactggtg cagtaccact aagggctaga ttgatcagct cttctggttt acggatacca
    13321 tcactgacag gacgattgat cagttctttt ccgtcaaagc gattcatctc tacttttttg
    13381 tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt cagcagtcaa tcggtttttg
    13441 attccttctg aaccgttcgt ttcgactttg atctccacac ccatttcttt cgcttttttc
    13501 ttcagagcat cttctgccat gtaagtgtga gcgatccctg ttggacaagc tgtaacggca
    13561 acaacgaatt ttctttctgg atgagcagaa gcttctgcag cttgtgtttc ttgttctgct
    13621 ttttcttctg cttctttttg ctcttcagct gtttggaaca agtcttggac ttcttctggt
    13681 gtatttgctt cttttaattt tcctacgaaa tctgcatcaa tcagcaatcg agatagtgct
    13741 gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag ctgcgatcat gaaaaacaga
    13801 taagttggct gtccgtctaa ggcttcataa tctactcctt tttggctttt tgcgaataaa
    13861 actgttgctt ctttaactgc actgtttttc gcgtgaggca tggcgattcc atcaccaaga
    13921 cctgtagaag tttgtgcttc gcgggctaga attccttctt tatatgtttc gatatcggaa
    13981 atccggccgg catcaaacat tttttggacc atttcatcga ttgcgccttt tttatcagtc
    14041 gcttgcaaat ccatgatcat cgcttctttg accattaaat ccttgatatt catgattatc
    14101 ctccttgaaa ctaatcaatt tttgtgattt caacttctgg cagcaactca tcaatcaatt
    14161 cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc gcaagcaact ccccatttaa
    14221 aggcttcgat tggatctttc gtacggctgt aagccccaat aaagccggca atcattgaat
    14281 cccctgcacc tactgaattt ttcaatgggc gaactaatac attcgtttta taagttcctt
    14341 gttccgtgaa gagcaaagca ccgtctccag ccattgagat cagcacattc tgtgcccctt
    14401 cagctagcaa ttttttacca tacggaatga tttcttctac agattggaag gttgtattgt
    14461 aaagctcagc taattcgtga ttgtttggtt tgatcaacaa tggtttctta acaagtgcat
    14521 tttttaggtc atcacctgtt gtatcaatca cgaaatctgc acctttttct cgaatgatat
    14581 caatcagttc ttgataaaat ccttttcgca ggctagccgg cgcgcttcct gacaatacga
    14641 cgatatctcc ttgtcctact gttcctaatg cttgcttcaa ctcatcgatt tcttcatctg
    14701 aaatagatgg gcccaaaccg ttaatttcag tttcagtatc tgattttagt ttgatattga
    14761 ttcttgtatc ttcttttacg gttgtgaaca aagactggat ttgttctttt tcaagccact
    14821 tcgaaataaa ttcgccagtg aaccctccta aaaaccctag tgctgtagat tgagaatcga
    14881 tccgtttcaa aatacgggaa acattgatcc cttttcctcc aggtaacttg aagtcttcct
    14941 tcattcggtt caaatcgcct agttttaacc catctacgcg tacgatataa tcaattgatg
    15001 gattaagtgt tactgtgtaa atcattgtat tgcctcctta attgttgtct tttcacggaa
    15061 ttcattaacg aattctaatg gacaacgctc ggttatgatc gtggctgctt ccaaaggtgc
    15121 aacagaggtg aaggacaact ggttgaattt ggtatgatca atcaatacaa atgtctcttc
    15181 ggaatttttc atggctaaac gtttaacagc tgcctcttct gggtccggag tcgtgaatcc
    15241 ttgttcaaga tgggcaccgt tcattcccat aaatgcttta ttaaaatgaa aattttttag
    15301 ttgttctatc gcattagatc ctaaaacagc attagttgcc agctttatcg ttccacctaa
    15361 aataatggta gcaagctcca aatcaactaa atttgccgca tgttgaacgg aatttgtcac
    15421 tactgtgatc tgttttcctt gcagaaaagg aatcatttct aacgtagtag agccagcgtc
    15481 taaataaatg acatcttcgt cattaatggt ttcagctgcc gatttggcga tttgctgttt
    15541 ttcgtgaacg tttttgagtg atttttcact catattctgc tcaaaattca ataattgctc
    15601 tttcttagct ccgccatgaa ctctttctag aaacccacta ttctccagct cttgtaaatc
    15661 acgtcttatc gttgattcag aagcattaaa aaggtttaca agatcttgtg atttcacgat
    15721 tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt tcctctgtaa gcattttcac
    15781 gatcacctca atacaaataa tagcatattt tttttctact cgcaatataa atcagtcaaa
    15841 aacgttcata aaaattataa atcagtcaaa aaaaaataaa aaaccagtca aaaagactgg
    15901 ttttttattt ttttgtctat catcaagccg ttttatcgtt ttttattttc gcttcttgtt
    15961 tttttcatat aaacagaagc aggttttctt tccttatgaa gaagataata ataaagtccc
    16021 atcccgccaa acatgaccaa aaaaccgatg actgatcgaa taggaaaatt aggaacaaaa
    16081 aagagacaga cggataataa tgatgccgga accattattc gataatagag ttgtttatcc
    16141 attttcttca cctcatattt tttgtaaagt aatcaattgt tctaattctc ggtaatctaa
    16201 aaattgcaca cgcagatgct gctcaccttt cttgacatcg aattggatac ttcgccgctt
    16261 tttctttatt cgtttgaagg taacatcaga gagtctatgt ttgatttcct ttaacggtaa
    16321 ttctgacagg gattctgaag gaacatagat tcttaaggat aaaagatact cttgatattt
    16381 ttcatctgtt acctctactt ctagatattc cctaatataa taacaataag cttcgattcc
    16441 attatccaaa gcaacagtcg gatcgataac tttatagcac atcatgcgcg agcgattctc
    16501 gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg cgaacacgcc tttctttata
    16561 atctccttgt tgaatatcat aactttttgt tacaaaaaat gaagcattct ttttaggaac
    16621 aggttgcatt tgtgcattct gatactggac agattggatc gtatggtcag tgtaaatgta
    16681 ttcaacgaat ggcggtttca ataaaccgac accattgtcc caagcaagat gatcaaaccc
    16741 aagctggtcc ctaaaatagg attgaaacat ttgattgttt ggcttttgcc agttaaaggg
    16801 gtctcgataa agctggatac atatgacttc gttttctaat tccttcactt tttctacttg
    16861 ttggatgtcg agaaaaatcg gtttagggat gatttgatga taatagcgac tataatacat
    16921 ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga tcgtatcctg gaaattggtt
    16981 gcaatcgact actacttccc cgttttgttg tttcatttta ggtaaatgtt cgatttcatc
    17041 ctttttctca aacaatagcc gttgagagat ctgttttgta ttgtgataga taaactcgct
    17101 ataagctcgc atataggcga acaccccatg agcaatcatt tttttctgaa tgaagtctcg
    17161 aatcaaaaat tgcttttctt tgaaaacaga gatagacaat acatttttta tcaataattg
    17221 attatcttcc aagcgaaaac tacattcgtt ctcgtcattt cctacaacta accaactttc
    17281 gtctcttacc gatgctagca ttattttttc ttgaagcttt cgaagatcat acggatccgc
    17341 ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac agacgtgtca aaatagccca
    17401 atcgcttatt tcctgttttt tttcttcttc atttaagtct gtgagctgga tagtcatcaa
    17461 taatttatct tcgccaatca ttttctcacc tctttaaaat ctttttataa tttaattata
    17521 accaacgata aagaaaaact aaaactttat gtcttagaaa ccaatttgga cgattacatg
    17581 atagactaag taaaaagaaa caaagaggtg tttacaatgg ggttgaaatt tgaacgtaca
    17641 gataatttag ataaactttt cgaatcattt gctgtcgatc cggaaaaaac agttgcacca
    17701 aaagaagaga tcgatcgatt tctaaaacca cagccacaaa atgaaaagaa aaaagacaag
    17761 aaacaaaagt aagttaagct caaaaaaaga cttaccagcc aaacttttat gaaagagttt
    17821 gactggtaag tcttttttat tctttttatg attcaattgc ttgttcgatt gctgcccacg
    17881 cttcttcttt tccctgtttt gtaacggaag aaaagagaat aaacgtgtca ttgcgatcaa
    17941 aatccaacgc ttttttgatt tcgctctcat gcttgttcca tttaccacga gggatcttat
    18001 ctgccttagt ggcaacaaca attacaggga tatcataata cttcaaaaat tgatacatct
    18061 ggatatcttc ttgagaaggc ttatgacgca aatctactaa agaaacgacc gcacgtagct
    18121 ggtctctagt cgttaaatat gtctcgatca tctttcccca ttttgctcgc tcagttttgg
    18181 atacttttgc atacccatat cccggaacat cgacaaaatg taatgcgttc tcgatcaaat
    18241 aaaaattcaa tgtctgggtc tttcctggct taccggaagt acgggctaaa ttctttcgat
    18301 caatcaatgt gttgataaaa gaagatttcc ctacatttga tcgtcctgcc agagctattt
    18361 ctggcaattg tgtatctgga tattgttttg gtgccactgc acttatgata atttcagctt
    18421 gatggacttt catggtctct ctccttaaca aggtgatgaa taaagcaagc ggctgtcgca
    18481 aaagccaaaa tgacttttac ggcagcctct tttcatcaac ccgctttttt ctctgaatct
    18541 ttatagatta ctgttggctc accagacaat tcagctgctt cttttgtgat aatcactttt
    18601 tcgatgtttt catttgaagg gatatcaaac atcacatcca tcatgatttc ttcaatgatg
    18661 gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt ttttcgcaat ggctctcaac
    18721 gcttcaggtt cgaattcaag ttcggtatta tctagagaaa gaagtttttg atattgtttg
    18781 actagcgcat ttttcggctc agtcaagatc cgtaccagat catctgttgt caatttctct
    18841 aaagctgcca taacaggtaa tcgtccgata aactcaggga tcaatccgaa ttttagtaaa
    18901 tcttcaggaa tgatatgctg catgacactt tcgtcttcag aaagtttttg gttttgtgta
    18961 ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa tcgtttcgat gccatcaaat
    19021 gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct gaataaattc ttgatgtgga
    19081 tgtttacgtc ctccctgagg tggcacgctt gcaacagttc cttctagaat tttcagcaag
    19141 gcttgttgga caccttcgcc ggaaacgtca cgagtaatag atacattttc gcttttacgc
    19201 gcaattttat cgatctcatc aatatagatg atcccttttt ctgcacgttc cacattgtaa
    19261 tcagcagatt gaagcaattt aagcaaaatg ttttctacat cttcccccac ataaccagct
    19321 tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat tcaatgtttt tgctaatgtt
    19381 tgtgctaaaa atgttttacc agaccctgta ggtccgatca agcagatatt gcttttttgc
    19441 agttcaacat catctgtgtt ttcttgtgct tgctgattga cacgtttata gtgattgtat
    19501 acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa tcacgtagtt attcaatact
    19561 tcaagaattt ctagtggttt agggacttca gtgaattcgc gtacagcttc ttcataaaat
    19621 tcttcatcaa tgatttcttt acataggtcg atacattcat tacagatgta gacccctgga
    19681 ccagctacga tctttttcac ttcttcttgg gttttcccgc aaaaagaaca gcgtacggtc
    19741 tcattgctac ttggattgtc atacatggtc ttcacccctt aacttgcaaa aagtatgcaa
    19801 gaaactatca aagactttac agtcttttac tatcataaca tagtttagta aaaagaaaaa
    19861 gtgtttaaca aaggatcaac tgacctatac ctttgcattt tttactctta acaaaaaata
    19921 aggaactgtg acaaaatcgt gtcacagtcc cttatttcga ataaacaatt ttctagaagt
    19981 agcttcttca gaaatgattt attacttgat ttctattctt tcgcttctgt cgctgtacct
    20041 gtgatcaatt cgattgcttt tttcattgtc acatcatgtt ccagcatatc ttctgtcaac
    20101 acgcgtttga tttgttcgat tggcatgttg tatgtttctg ataattcttg gatttctgca
    20161 ttgatttctt cttctgttgc ttgaagattt tcagcagctg caattgcttc gatcacaaga
    20221 tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt tatgcaagtc ttcttctgta
    20281 gaaccagtta attgatagta catttcagga gagatacctt gacgttgcat gttgttcaag
    20341 aattcgtcca ttgaacgatg tacttcatca tgaaccatca cgtgtggcaa ttcaacgatt
    20401 tctgcgtttt caacagccat acggatcgct gcttcatctt ttgcatcgtc cgctgctttt
    20461 tcttttgttt ctactaattc tttgcgatat ttttctttca attcgtctaa tgttgatact
    20521 tcgtcatcaa catcttttgc gaattcgtca tctaattcag gcacttcttt tgcttttact
    20581 tcatggattg tcactttgaa gactgcttct tttccagcaa gatcttctgc ttgataatct
    20641 tctgggaaag ttacatttac atctagactt tcacctgatt tatgaccaac taattgatct
    20701 tcaaaacctg gaatgaatga gcctgaacct aattcaagtg agtaattttc gccttttccg
    20761 ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga ttactactgt atcgccattt
    20821 tcagctgcag catcttcttt gataactaat tcagcttgtg cttcttgttc acgtttgata
    20881 cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt tttcaacaac tagatcttta
    20941 tattgtccta attttacttc tggtttcact gttacttcag cagtgattac ccagtcttga
    21001 cctttttcca tgctttcaac atcgattttt ggttgagaaa caggatcgat gccagcttct
    21061 ttgacagcag cttcataagc ttctggtaat acagcattca atgcatcttc atataaagct
    21121 tcttcaccgt acatacggtt gaatacttga cgagaaactt tccctttacg gaatccaggt
    21181 acgttcaagc tttttttcac tttattaaac gcagttgtta atcctttttg gatttcgtct
    21241 tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt ttttttcaaa tgttgcagtc
    21301 atttaattcc ctccgagttt agaaattcat tttatacatc aaaaacgttt catgcatacc
    21361 tttaaatagt acactaaccc tttcaaattg taaacatatt gaatggatag aatccttgtt
    21421 tttcataaca tttttatctt tttcactgat aaaatctaat ggaagaagat catttgctgc
    21481 aaaatttttt ctcttttttt taaaaaatct tgtttttcat cttctgtacc ttcatacatc
    21541 gtttttcctg tacacgcctc tacagtcagt tgaaaccagc gcttcgggtc ttcgatgtat
    21601 gtatcataga caggataaac aattgcagat tgcaacgtga attcttcatt taacacggaa
    21661 ctaagcagcg catcttcatt accgtaacga tctgaaaata cacataattg ctggtaactt
    21721 gcctgctgct gcggctttgg cagatctttt ggtatgactt tgactaactt ttcttcaatc
    21781 gtcaaataga aaacaggatg gcattcgttc aattgcctaa gtgtctccag taattttgcc
    21841 cttgctaaaa tatggacctc tggcgataca ataagtttac tcgctgtttg gataaactca
    21901 tgataaggaa gctgttcgat ctcatcaata gacgcaagct gacgtgcagg ttccaacgaa
    21961 ggaagttcct ccgatttatt gtggagtgcc ttgatcttct gcctctccat ttgtccggat
    22021 aattcttctg caaattggat tttttcaatg acccgctgct cgaaagcttc ataaaaatct
    22081 gtacttgcta tcaattttct agcatataaa aaatcttgtg tactgattaa caagtcaaaa
    22141 taaaattcag caaactcctc attttgaaga tactgttttt catgcaagag tgcttgcttc
    22201 aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa caagcaggct attagcctca
    22261 aagctttctt tcaactcgta agctcgttga aataattctt ttgcctgctg atattgctgg
    22321 tttgctagtg cttgttgtgc tactcgcatt aaattctcat atttttctgg aaaatcaatc
    22381 ggcttcacgc agtgtcctcc tcttgctcat gttcagctca tgttcaatag tagcaaaaca
    22441 aaaaaagcat gacaattaaa aataactgaa gcaccctcta aaagctagat tttagatctg
    22501 acttttagaa ggtgctttca actgttactg ctttttattg ctcatcagct gttgacttcc
    22561 tggttgaatg ctttgatcag attgatcgta acaacacttg tattagtgaa aaaatcgcct
    22621 agatgttgct ttttctgatt aaagattgct ggaagatacc ctaacatcag caaaggaaat
    22681 tttagaatga accggcaagc accttctcta actaaaacgg ttgaccagct aagttcttct
    22741 tcatcaaagc tgatgactcg aataccaaag atcattttcc caatcgtttg accatgattg
    22801 aatttcgtca ataaaataaa atacgctaga taaataagta aggctgtgaa tccgtagata
    22861 ctcagataag aatctgaaaa attaagccaa cctagatttt tggcaatacc aagtgttaaa
    22921 cgcgtaatag ctccgataca caaaagatcg accaagaaag caaacattcg gataaaaaaa
    22981 cctgcataga aatacttagg aaaatcattt gcttttggcg gttcatttct tgtgtccgta
    23041 tactttttcc atttacgttg tttctttttg atttcttcgg gcgtcaaagg ctctctttca
    23101 aaagagctca gtaccttttg ggaaaagttg gagtcaggtc ctacagcagg ctgctgttgc
    23161 ttatcttttt cttgttcctt attttcattt gaaagctctg acatgcttac tcacctccgt
    23221 aataatacat tgcttttggt gattccgctg tccctagatt ctcaagaacg gataagatct
    23281 gactagtctc agaaggcttc aatccctgga attctgctag tttacttcct aaccatgtac
    23341 tagcaaaccc agatggcgtg gaagaatatt ctactagttc cgcatcttca agctgttgtt
    23401 cctttcgcat agcttttaaa gcatcttcag gaaatcccaa ttcatcgact aaacctactt
    23461 cttttgcttg gacaccatca taaatccttc catcagcaat ttttttgact tcttcttcag
    23521 atttattccg tccctcgctg acgattgcta caaaacgact atatgcgctg tcgatatatg
    23581 cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt tcctgaaccc atatctttca
    23641 acgctccgct tttaactgtt gtatcttcca cacccagttt ttctagaagt cctgaataat
    23701 tcaatcctga catgatcaca ccaattgagc cggtcactgt ttcttctgtt gcaaagattt
    23761 tatctgcttg agcagaaata tagtagccac cgctagctgc catgtttttc atgctgacat
    23821 acattgggat atcatgttct ttacgaatcg tatccaattt cttagcgatt tccgcacttt
    23881 cgtagacacc gccaccagga gaatttactt ctaaaaagat acctttgact gttggatctt
    23941 cttcaatcgc cttcagttgt tccataaaat ttttatgatt gtaaccttca gaagcaaata
    24001 aacctgaagc ccctcccgca gcaatcgttc catctactga taattttacg attttttgcg
    24061 tactgtctcc ctcttccaac acatttgaag ctagttcttc atccccatat aaccaactgt
    24121 tcaatccctc aatttgatcg ttttcctgag gcttagtcaa actagctgaa actaacgaaa
    24181 ccacaaataa tcctgcagca atcagaactg ctgcccaacg tctcttattc attctttctc
    24241 ctttctaata caagtttttt atgacgatca cagtttctga ctgctcagaa gtgtcgaacc
    24301 gctctgcttt ttcttcccgc gcatacccgg aaaacgactc ttttgtcaat tcatcttcta
    24361 ccgtaaactc acgagcttca gcaacttcca aggcatcagg tgcgatttct tggatgctat
    24421 cccagccagt atctaaaaat attttcgtat catctgatac accagctgat gcttcgaatt
    24481 tggacaattg tttttttaac attcccagtg tcatgatctc aatttttgaa tccattttta
    24541 ctcctcctct atccgattac tttctattgt accgaactcc tgaaaaagat gatagataaa
    24601 aacccattat tttttgacat aaaaagagca tttgacaaag catttgatat ataccaattt
    24661 attttttatt ctttttttgt tgacaagaac attcgcaaaa ggttatgatt ttaaatgttt
    24721 ccagataaaa aacaaaagag gaggatagtt atacctttta ggatagcgcc aggtctgtgt
    24781 aaacagatga cgaggagagg gtttatcgaa agattcggcg gatggcccta gggactgcac
    24841 tctacagaaa atccacaaaa aatatctgca aagataaaac aaaaggattt tcctgcagct
    24901 ggaagcattc atacgaattc attatagaaa gtaggatgtt ttttatgtgc ggaattgttg
    24961 gaatgattgg attaaaaaat gtaacaccag gattgattga cggtctagaa aaattagaat
    25021 accgtggtta tgattcagct ggtatctttg tctcagatgg cacaactgat tatctggtca
    25081 aagcacaagg cagaattcaa aatttgaaaa acaaaataaa tattgatact acaggaaata
    25141 taggtattgg tcatacacgc tgggcaactc acgggcaacc ttctgaagaa aacgcccatc
    25201 cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa tggcgtaatc gaaaactttg
    25261 aagaacttaa aattgcttat ttagcaaatg accactttat tggtgagacc gatactgaga
    25321 ttatcgctca tctaattgaa actttcgcga aggatactac aacgcaagag gcgtttttaa
    25381 aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg acagcacctg
    25441 atgttattta cgtcgctaaa aataaaagtc cactattaat cggactcggc gatggattca
    25501 atgtgattgc cagcgatgca atggctatgc ttgctcatac gaaggaattc gttgagattg
    25561 aagatgaaga gatggttacc gtcacatcag aaaaagttat tattcaaaat tttgcaggtg
    25621 acatcgtaga acgttcttca ttcgaagcac aagtagattc ttcagatatc gagaaaggta
    25681 cctatccttt ttatatgctg aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg
    25741 ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg gtcgatacat
    25801 tgtctgatag cgatcgaatc tatatcgttg cttgcggaac tagttaccat gcggggttag
    25861 caggtaaaca gactctggaa aaattgacac agatccctgt tgaggttcat ttagccagtg
    25921 aatttggcta taacacacct cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa
    25981 gtggcgaaac agcagatagc cgccaagttc tggtaaaaat caaccgtctc ggctaccctt
    26041 cacttacgat tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc tttacactgc
    26101 tattgcatgc tggacccgaa atcgcagtag catcaacgaa agcttatact gcacaaatag
    26161 ctgtactaac tttattagcc aaagctatcg gtgataaaaa agaatttgaa acttctcttg
    26221 tattcgatat cactcatgaa ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc
    26281 aaaaagatta tttagaagag ttggcagaag tatatctcag cgatacaaga aatgcctttt
    26341 acattggacg tggcgctgac tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa
    26401 tcagttatat ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga acgattgctt
    26461 tgatcgaaga aggaacacca gtgattggaa tcatcagtga agaagtgact ggtgcgcata
    26521 ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt attgtctcag
    26581 agggattaga aaaggaaagt gaccagctgg tgctaccaac tgtccaccct tatctaacta
    26641 ctctaaccac agtcgtaccg actcaattac ttgcatatta cgcaacgcta ttacggggat
    26701 tcgacgttga caaaccacgt aatttagcga aatcagtgac agtagaataa taaaagaaca
    26761 aggaccagtg tcagacatct gacactggtc cttgttcttt tattttttat gaaaatatct
    26821 cttaatccag tccgatttct tctttgacta ctgccgcgat tttatcgaca tagtaattaa
    26881 ctttctcatc agtaggcgct tctgccatca ctcgaagtaa tggttctgta ccggaaggac
    26941 gaactaggat tcttccttct gatcccattt cagcttctgc ctcttcaatc acttttttga
    27001 ttgccggaac atccatcgca ccgtttttat cacttactcg gatattcact aatttttgcg
    27061 gatagattgt tacctctgct gcaagctctg ataatttctt acctgtctgt ttcatgatat
    27121 tcagtaactg aattcctgaa agcatcccat ctccagttgt gttaaaatct aagaaaatca
    27181 tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt gcgcatttct tctacaacgt
    27241 aacgatcacc aacttgtgta acgacatctt tcaaaccgat tccttccacc gctttgtgga
    27301 atcctaagtt actcatgaca gtcgtaacga tcgtatcttt tttcaatcga tttttttctg
    27361 ccaagtattt agcacagata aacatgattt tatcaccgtc gacaatgttt ccaagttcat
    27421 ccaccgcaat aatacgatcg ccatcgccat cgaaagctag tccagcatct gctccttttt
    27481 caacgaccat ctcagctaaa cgttctggat gggtagagcc tacgccatca ttgatattta
    27541 acccgtctgg atttgtgccc atggtataaa aatctgtttc aagatcggca aacaaacgat
    27601 taacacttgt tgaagttgct ccgtttgctg catcgacaca aatggtcaaa ccggaaaggt
    27661 caccgttgat cgtttgtaca aggaattgag aatatttcaa taagccttct ggaaattctt
    27721 ctacagttcc caatccttca gctgatggac gaggcaattc atccacttca gcatctaata
    27781 atgcttcaat ttctagctct tggtcatcta ctagtttaaa accatctgca ccgaaaaatt
    27841 tgattccatt atcttgtgct ggattatgag aagcagaaat catgacccct gcgcttgctt
    27901 tttgcaaacg tgtcagataa gcaacacctg gtgtagagat cacacctagc tggaacactt
    27961 cgatacccac cgataaaagt cctgaaatca gcgcttcttc taacatttga cctgagattc
    28021 ttgtatcccg ccctactaac acgcgtgggc gttttccttc ttcttcatgc tggcttagga
    28081 cataacctcc gcaccgtccc agcttaaatg caagttctgg cgtaagttcc ttgttggctt
    28141 caccgcgtac gccgtctgtt ccaaaatatt tacccatagt tgtaaaatta ctcctttaac
    28201 ttaattttct gaggaatttg ttctgctcat ttgtgttgat tcgcttgttt ctgatgtttc
    28261 ttctgtactt gtggtcgagc tgcttgtccc agattccgaa ctggaagaaa tggttgttgt
    28321 cgatggttcc gttcctgaat cagcagatga aatacttcta ctcgtacttg tattttcttg
    28381 tgtccgactg ctcgtcgaag cagagctact gcttgacgtt tgcaacacgg gtgtaatctc
    28441 cacactaact gattcgggct tgatacttaa cccctcttct actggtattt caattttacg
    28501 agttgttcga tgattgatac ctgagacatt gatcggtaca gcaatactgt caccgatcga
    28561 tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt tttgaaaaac tgaaatcata
    28621 gctttctacc ccggcagctg gtgttccttg ctgtatccca taaagtcgta ccgacttgct
    28681 tggcttgatc gaatcagctc tcactgttac ttgcactgga tcagagacga tacttaatac
    28741 ttcccctgct gcattcagcg cctgtattgg gacctcacta ttaatcccat catctgtgac
    28801 catcgaagcg tccacatcag ctactactcg tttgatttct gccaatgttt tgtcacctgt
    28861 agtgatagag acttttttag gactaacgga agtatcatca agttcatatc cagatggtgt
    28921 gaacgttgaa ggaatcaacg tttccacatc gaaatctttt attacctttt tctcaaccgt
    28981 tactgtaata gtcgatggtt cgatctttgc tgtgacagca ctgctcaaat tttgaatttt
    29041 tagcggaacc tcatgtgttc cttcgcccag cttagtgata tctgctgtga cgcggaacat
    29101 ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg tttgcactgg aaagtttgac
    29161 agtcactgca ttttcatatc catggatata gtagcgcttt gaatcataag aaatcttgat
    29221 tggcacccca gtaactgttt gattatacgc ttcatttcca gaaagcgtat ttggaacgga
    29281 ctggccattt gcattgaagt acagcgcaat actgaacagc aatgcaataa aactgtaaag
    29341 aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc ctcccttagt cataccgtct
    29401 aggaaagatt gaatcacgtt tttcttgttt tttgttttct cttcagcaat cagttctcta
    29461 cgcaaaattg ccaaatattc ttgctgggat aagccagcca tcagttcatt atctaatgta
    29521 atgctgacat ccccagtttc ttcagagaca acgatggtaa tcgcgtcgct gacttcgctt
    29581 ataccgacag ctgcccggtg tcgagtcccg aattcttttg gaatcaacat actttctgat
    29641 agaggaagat aggcgcttgc tactgcgatc tttccttctt ttacaataac tgcaccgtca
    29701 tgcagcggtg tattaggaat aaagatattg atcagcagct ctccagtgat atctgcatct
    29761 aatgcaattc ctgtctcaat atactcatct aatccagtat gccgttcgat agtaattagc
    29821 gcaccgattt ttcgcttgga catatattgg atggctttgt caaaagacaa gatcatcttc
    29881 tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg aactgcgtcc aagatgttcc
    29941 agtcctctcc ggatttccgg ctgaaaaatc acgatagcag ctatcacccc gtacgtaatg
    30001 acttggttca tcagccaaga caaggtgtgc agaccaatga tttccgctac gattcggata
    30061 acaataaaaa tagctacacc tttcagaagc tgaatcgctt ttgtgcctcg cagcagttgg
    30121 atcagtttgt atacaagata ccagacaacc agaatatcaa gtatgttcac caaaaaattc
    30181 agagagaaca agtcaccgga aaacatttgc tgccaatagt ccaaattaaa aagttccccg
    30241 atttgaaatg acataaaaag cctctcttct cttttgagat ttcctctcta gtatatcata
    30301 tgttttaatt ttagaggctg atctgctcgt atttttcttg agaaaaacgc ataattttca
    30361 ggaaaattgc aagtatgcgt tttatttttc tctgcaataa atatttttaa acttcagaga
    30421 att
//
LOCUS       DACIFS010000034        29108 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.034, whole genome shotgun sequence.
ACCESSION   DACIFS010000034 DACIFS010000000
VERSION     DACIFS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.034"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..1029)
                     /locus_tag="IYA83_001537"
     CDS             complement(<1..1029)
                     /locus_tag="IYA83_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8586972.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="IYA83_001538"
     CDS             complement(1111..1800)
                     /locus_tag="IYA83_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ8586973.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IYA83_001539"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IYA83_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ8586974.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /locus_tag="IYA83_001540"
     CDS             complement(2327..2830)
                     /locus_tag="IYA83_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ8586975.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IYA83_001541"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IYA83_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ8586976.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IYA83_001542"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IYA83_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ8586977.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4798..5466
                     /gene="sdaAB"
                     /locus_tag="IYA83_001543"
     CDS             4798..5466
                     /gene="sdaAB"
                     /locus_tag="IYA83_001543"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ8586978.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5479..6351
                     /gene="sdaAA"
                     /locus_tag="IYA83_001544"
     CDS             5479..6351
                     /gene="sdaAA"
                     /locus_tag="IYA83_001544"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ8586979.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6442..7461)
                     /locus_tag="IYA83_001545"
     CDS             complement(6442..7461)
                     /locus_tag="IYA83_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ8586980.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7634..8521)
                     /locus_tag="IYA83_001546"
     CDS             complement(7634..8521)
                     /locus_tag="IYA83_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ8586981.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IYA83_001547"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IYA83_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ8586982.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IYA83_001548"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IYA83_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ8586983.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IYA83_001549"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IYA83_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ8586984.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /locus_tag="IYA83_001550"
     CDS             9752..10396
                     /locus_tag="IYA83_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ8586985.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="IYA83_001551"
     CDS             complement(10452..10955)
                     /locus_tag="IYA83_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ8586986.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11552..12517
                     /locus_tag="IYA83_001552"
     CDS             11552..12517
                     /locus_tag="IYA83_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ8586987.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12616..13788)
                     /locus_tag="IYA83_001553"
     CDS             complement(12616..13788)
                     /locus_tag="IYA83_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586988.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13775..14920)
                     /locus_tag="IYA83_001554"
     CDS             complement(13775..14920)
                     /locus_tag="IYA83_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8586989.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14934..15866)
                     /locus_tag="IYA83_001555"
     CDS             complement(14934..15866)
                     /locus_tag="IYA83_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8586990.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15983..17062
                     /locus_tag="IYA83_001556"
     CDS             15983..17062
                     /locus_tag="IYA83_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ8586991.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17059..17688
                     /locus_tag="IYA83_001557"
     CDS             17059..17688
                     /locus_tag="IYA83_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8586992.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17798..18025)
                     /locus_tag="IYA83_001558"
     CDS             complement(17798..18025)
                     /locus_tag="IYA83_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ8586993.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18128..20716)
                     /locus_tag="IYA83_001559"
     CDS             complement(18128..20716)
                     /locus_tag="IYA83_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ8586994.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20736..21395)
                     /locus_tag="IYA83_001560"
     CDS             complement(20736..21395)
                     /locus_tag="IYA83_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ8586995.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IYA83_001561"
     CDS             complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IYA83_001561"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ8586996.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22168..23034)
                     /locus_tag="IYA83_001562"
     CDS             complement(22168..23034)
                     /locus_tag="IYA83_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ8586997.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23143..23784
                     /locus_tag="IYA83_001563"
     CDS             23143..23784
                     /locus_tag="IYA83_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ8586998.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IYA83_001564"
     CDS             complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IYA83_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ8586999.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25225..26124)
                     /locus_tag="IYA83_001565"
     CDS             complement(25225..26124)
                     /locus_tag="IYA83_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ8587000.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26126..26923)
                     /locus_tag="IYA83_001566"
     CDS             complement(26126..26923)
                     /locus_tag="IYA83_001566"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ8587001.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26901..27605)
                     /locus_tag="IYA83_001567"
     CDS             complement(26901..27605)
                     /locus_tag="IYA83_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ8587002.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27736..28311
                     /locus_tag="IYA83_001568"
     CDS             27736..28311
                     /locus_tag="IYA83_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ8587003.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28429..29106
                     /locus_tag="IYA83_001569"
     CDS             28429..29106
                     /locus_tag="IYA83_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ8587004.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6169 c         5232 g         9143 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
     4621 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
     4681 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
     4741 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
     4801 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
     4861 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
     4921 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
     4981 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
     5041 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
     5101 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
     5161 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
     5221 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
     5281 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
     5341 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
     5401 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
     5461 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
     5521 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
     5581 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
     5641 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
     5701 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
     5761 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
     5821 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
     5881 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
     5941 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
     6001 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
     6061 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
     6121 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
     6181 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
     6241 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
     6301 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
     6361 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
     6421 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
     6481 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
     6541 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
     6601 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
     6661 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
     6721 cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac catctgcccc
     6781 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
     6841 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
     6901 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
     6961 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
     7021 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
     7081 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
     7141 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
     7201 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
     7261 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
     7321 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
     7381 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
     7441 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
     7501 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
     7561 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
     7621 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
     7681 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
     7741 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
     7801 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
     7861 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
     7921 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
     7981 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
     8041 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
     8101 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
     8161 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
     8221 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
     8281 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    11521 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    11581 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    11641 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    11701 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    11761 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    11821 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
    11881 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    11941 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    12001 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    12061 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    12121 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    12181 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    12241 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    12301 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    12361 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    12421 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    12481 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    12541 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    12601 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    12661 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    12721 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    12781 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    12841 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    12901 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    12961 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    13021 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    13081 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    13141 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
    13201 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    13261 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    13321 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    13381 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    13441 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    13501 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    13561 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    13621 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    13681 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    13741 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    13801 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    13861 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    13921 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    13981 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    14041 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    14101 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    14161 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    14221 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    14281 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    14341 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    14401 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    14461 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    14521 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    14581 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    14641 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    14701 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    14761 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
    14821 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    14881 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    14941 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    15001 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    15061 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    15121 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
    15181 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    15241 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    15301 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    15361 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    15421 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    15481 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    15541 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    15601 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    15661 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    15721 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
    15781 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    15841 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    15901 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    15961 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    16021 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    16081 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    16141 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    16201 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    16261 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    16321 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    16381 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    16441 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    16501 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    16561 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    16621 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    16681 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    16741 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    16801 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    16861 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    16921 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    16981 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    17041 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
    17101 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
    17161 tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac
    17221 atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc
    17281 aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt
    17341 tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc
    17401 aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa
    17461 atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag
    17521 cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg
    17581 tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta
    17641 cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac
    17701 acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc
    17761 aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag
    17821 gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt
    17881 ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt
    17941 ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta
    18001 atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat
    18061 atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc
    18121 tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa
    18181 tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa
    18241 cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt
    18301 ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta
    18361 gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc
    18421 cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat
    18481 tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag
    18541 tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg
    18601 tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca
    18661 tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat
    18721 tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca
    18781 ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga
    18841 atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg
    18901 tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca
    18961 ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat
    19021 cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa
    19081 tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg
    19141 gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc
    19201 gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta
    19261 cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat
    19321 tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat
    19381 caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc
    19441 cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc
    19501 gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt
    19561 ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca
    19621 cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt
    19681 cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc
    19741 gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc
    19801 gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag
    19861 tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat
    19921 caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg
    19981 tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga
    20041 tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat
    20101 ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga
    20161 tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga
    20221 acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct
    20281 cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat
    20341 cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt
    20401 tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg
    20461 ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca
    20521 ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga
    20581 ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt
    20641 tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca
    20701 tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt
    20761 tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa
    20821 ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac
    20881 tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct
    20941 acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata
    21001 cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa
    21061 tgattacgca aattcggcag attatccgga tatttctggt acatttcatc gatcgactga
    21121 ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga
    21181 cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa
    21241 taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa
    21301 ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat
    21361 tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt
    21421 tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat
    21481 tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc
    21541 gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg
    21601 ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc
    21661 tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa
    21721 tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata
    21781 tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa
    21841 cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt
    21901 ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct
    21961 ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta
    22021 tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt
    22081 atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc
    22141 tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt
    22201 tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc
    22261 tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt
    22321 ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc
    22381 agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata
    22441 aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc
    22501 atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac
    22561 cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc
    22621 ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc
    22681 attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac
    22741 atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata
    22801 catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt
    22861 aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt
    22921 tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa
    22981 cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc
    23041 ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga
    23101 tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc
    23161 gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg
    23221 tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta
    23281 agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac
    23341 ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa
    23401 ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag
    23461 gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt
    23521 gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt
    23581 gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg
    23641 cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc
    23701 actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc
    23761 aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat
    23821 gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat
    23881 tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta
    23941 ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg
    24001 ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt
    24061 tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg
    24121 tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa
    24181 gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca
    24241 gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta
    24301 aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg
    24361 ctgcctttac tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat
    24421 cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca
    24481 aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg
    24541 ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag
    24601 aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct
    24661 gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct
    24721 tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg
    24781 tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat
    24841 cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca
    24901 agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca
    24961 tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg
    25021 ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt
    25081 ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa
    25141 tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt
    25201 catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc
    25261 tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag
    25321 atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc
    25381 atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc
    25441 tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc
    25501 agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg
    25561 ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa
    25621 ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc
    25681 aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac
    25741 atggattact tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat
    25801 tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac
    25861 cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc
    25921 atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa
    25981 gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa
    26041 cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt
    26101 ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat
    26161 gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat
    26221 aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt
    26281 ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca
    26341 aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac
    26401 taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag
    26461 aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg
    26521 ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt
    26581 ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt
    26641 cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt
    26701 aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt
    26761 agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct
    26821 catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt
    26881 ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt
    26941 tcgctttctt tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg
    27001 gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac
    27061 tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct
    27121 tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga
    27181 atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc
    27241 accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg
    27301 agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca
    27361 aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt
    27421 ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg
    27481 acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt
    27541 ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt
    27601 tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag
    27661 ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa
    27721 aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag
    27781 caagagtacg agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag
    27841 actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg
    27901 atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt
    27961 ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt
    28021 ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca
    28081 ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt
    28141 ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta
    28201 ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct
    28261 tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc
    28321 attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga
    28381 aagaatctag tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc
    28441 tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa
    28501 tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct
    28561 ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat
    28621 cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg
    28681 gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc
    28741 ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc
    28801 ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt
    28861 tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac
    28921 aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt
    28981 tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa
    29041 catttacata caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct
    29101 aattaaaa
//
LOCUS       DACIFS010000035        28032 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.035, whole genome shotgun sequence.
ACCESSION   DACIFS010000035 DACIFS010000000
VERSION     DACIFS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.035"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            513..1220
                     /locus_tag="IYA83_001570"
     CDS             513..1220
                     /locus_tag="IYA83_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8587005.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1279..1923)
                     /locus_tag="IYA83_001571"
     CDS             complement(1279..1923)
                     /locus_tag="IYA83_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ8587006.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2049..2336
                     /locus_tag="IYA83_001572"
     CDS             2049..2336
                     /locus_tag="IYA83_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587007.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2444..2764
                     /locus_tag="IYA83_001573"
     CDS             2444..2764
                     /locus_tag="IYA83_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587008.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2845..4158
                     /locus_tag="IYA83_001574"
     CDS             2845..4158
                     /locus_tag="IYA83_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ8587009.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4155..4874
                     /locus_tag="IYA83_001575"
     CDS             4155..4874
                     /locus_tag="IYA83_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ8587010.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            5002..5553
                     /gene="raiA"
                     /locus_tag="IYA83_001576"
     CDS             5002..5553
                     /gene="raiA"
                     /locus_tag="IYA83_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ8587011.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5775..8309
                     /gene="secA"
                     /locus_tag="IYA83_001577"
     CDS             5775..8309
                     /gene="secA"
                     /locus_tag="IYA83_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ8587012.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8435..9536
                     /gene="prfB"
                     /locus_tag="IYA83_001578"
     CDS             join(8435..8497,8499..9536)
                     /gene="prfB"
                     /locus_tag="IYA83_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ8587013.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9641..10327
                     /gene="ftsE"
                     /locus_tag="IYA83_001579"
     CDS             9641..10327
                     /gene="ftsE"
                     /locus_tag="IYA83_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ8587014.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10311..11204
                     /locus_tag="IYA83_001580"
     CDS             10311..11204
                     /locus_tag="IYA83_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587015.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11356..12804
                     /gene="cls"
                     /locus_tag="IYA83_001581"
     CDS             11356..12804
                     /gene="cls"
                     /locus_tag="IYA83_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ8587016.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13141..13998
                     /locus_tag="IYA83_001582"
     CDS             13141..13998
                     /locus_tag="IYA83_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ8587017.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14113..15033
                     /gene="pstC"
                     /locus_tag="IYA83_001583"
     CDS             14113..15033
                     /gene="pstC"
                     /locus_tag="IYA83_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ8587018.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15033..15917
                     /gene="pstA"
                     /locus_tag="IYA83_001584"
     CDS             15033..15917
                     /gene="pstA"
                     /locus_tag="IYA83_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ8587019.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15932..16738
                     /gene="pstB"
                     /locus_tag="IYA83_001585"
     CDS             15932..16738
                     /gene="pstB"
                     /locus_tag="IYA83_001585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587020.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16754..17512
                     /locus_tag="IYA83_001586"
     CDS             16754..17512
                     /locus_tag="IYA83_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587021.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17525..18202
                     /gene="phoU"
                     /locus_tag="IYA83_001587"
     CDS             17525..18202
                     /gene="phoU"
                     /locus_tag="IYA83_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ8587022.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18439..19470
                     /locus_tag="IYA83_001588"
     CDS             18439..19470
                     /locus_tag="IYA83_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ8587023.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19467..20234
                     /locus_tag="IYA83_001589"
     CDS             19467..20234
                     /locus_tag="IYA83_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ8587024.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20334..21902
                     /gene="liaX"
                     /locus_tag="IYA83_001590"
     CDS             20334..21902
                     /gene="liaX"
                     /locus_tag="IYA83_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ8587025.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22202..22519
                     /locus_tag="IYA83_001591"
     CDS             22202..22519
                     /locus_tag="IYA83_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ8587026.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22519..22869
                     /locus_tag="IYA83_001592"
     CDS             22519..22869
                     /locus_tag="IYA83_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ8587027.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23036..23974
                     /locus_tag="IYA83_001593"
     CDS             23036..23974
                     /locus_tag="IYA83_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ8587028.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23989..24819
                     /locus_tag="IYA83_001594"
     CDS             23989..24819
                     /locus_tag="IYA83_001594"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ8587029.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24863..25888
                     /locus_tag="IYA83_001595"
     CDS             24863..25888
                     /locus_tag="IYA83_001595"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ8587030.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25903..26841
                     /gene="galU"
                     /locus_tag="IYA83_001596"
     CDS             25903..26841
                     /gene="galU"
                     /locus_tag="IYA83_001596"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ8587031.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26966..27406
                     /locus_tag="IYA83_001597"
     CDS             26966..27406
                     /locus_tag="IYA83_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ8587032.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27409..28011
                     /locus_tag="IYA83_001598"
     CDS             27409..28011
                     /locus_tag="IYA83_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ8587033.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9278 a         4817 c         6065 g         7872 t
ORIGIN      
        1 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
       61 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
      121 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
      181 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa
      241 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
      301 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
      361 gacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
      421 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
      481 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
      541 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
      601 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
      661 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
      721 ttgcacgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
      781 aatcacaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
      841 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
      901 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
      961 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     1021 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     1081 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     1141 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     1201 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccgtattaga aataaaagag
     1261 aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg
     1321 accctcttca aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc
     1381 agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt
     1441 tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat
     1501 catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg
     1561 agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa
     1621 atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga cttccaacat
     1681 cggtatgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt
     1741 ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc
     1801 ttgaatgaaa ctttttgctt cgtcctctga ttctattcgt ctaagagaac agataaagcg
     1861 tgattttttg atctcgatct cactttgtcc attttctttt attgtacgat agcgctcgat
     1921 catcttgtca cctctttttt cattttcttt attcagtata aataaagttc taggttgttt
     1981 caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg
     2041 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     2101 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     2161 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     2221 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     2281 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     2341 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     2401 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     2461 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     2521 tgctgaagca aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa
     2581 caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa
     2641 agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc
     2701 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     2761 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     2821 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     2881 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     2941 gtggaacgtt tgagcaactg gattctttgt catcgctgtg gaatgagaac gtccgtcatt
     3001 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     3061 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     3121 tttttttgga caggacagct tactgaaaaa caaacggaaa tctcaaagag aatactttgc
     3181 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     3241 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     3301 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     3361 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     3421 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     3481 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     3541 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggagatc
     3601 aagcagacgt ttgacatcca caaacttgct ttacgctttc atcggcgttt gcttcctgaa
     3661 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     3721 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     3781 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     3841 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     3901 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     3961 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     4021 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ttttttctat
     4081 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     4141 gggaagaaga tactgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     4201 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     4261 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     4321 atctagagag tacgaaaaag aaagtaaatt ctatccccgt ttactgcagt gactgtcaag
     4381 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     4441 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     4501 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     4561 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     4621 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     4681 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     4741 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     4801 ctcttttcca cgcagcagat tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt
     4861 ctttggcgag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     4921 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     4981 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     5041 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     5101 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     5161 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     5221 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     5281 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta
     5341 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     5401 ctttcattga aaccaatgga tagtgaagaa gcagtcttgc aaatgaacat gctgggacat
     5461 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
     5521 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa
     5581 cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga
     5641 aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca attatctata
     5701 actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg
     5761 agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac
     5821 taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat
     5881 taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag
     5941 aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg
     6001 tattaggttt atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa
     6061 atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc
     6121 taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc
     6181 gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata
     6241 tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta
     6301 cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa
     6361 tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg
     6421 aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa
     6481 gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat
     6541 caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg
     6601 ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag
     6661 ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg
     6721 ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag
     6781 caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca
     6841 ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa
     6901 cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc
     6961 gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc
     7021 acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa
     7081 ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc
     7141 atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc
     7201 aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag
     7261 gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc
     7321 gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac
     7381 agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaag
     7441 ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga
     7501 cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa
     7561 acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac
     7621 aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga
     7681 caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa
     7741 gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata
     7801 tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat
     7861 ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg
     7921 tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgacgcaatg gatcaattac
     7981 gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag
     8041 aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct
     8101 ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt
     8161 ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa
     8221 tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata
     8281 agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc
     8341 tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata
     8401 taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct
     8461 cctgacagaa atgcaggaaa aggtcacaag tttcaggagg tctctttgac ttagatcagc
     8521 tggaagaaga catcgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgaca
     8581 gcgaagccgc acaaaaagtg atcagtgaaa ataacgctaa taaagaaacc tatgaccaat
     8641 tccattctct tgccgaagaa ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag
     8701 aacctgatgc agcaatgcaa gaagaagcag aaacacgtat caatgcatta gatgaaaaaa
     8761 tgaagacgta tgaattatct atgctgttag acggacctta tgatcgaaac aatgcgatcg
     8821 tcgaattgca tccaggggct ggcggaacgg agtcccaaga ctggggcagc atgcttttac
     8881 gtatgtatac acgttgggca gagcagcatg gctatcaagt agaaacacta gattatcaag
     8941 caggtgatga agcagggatc aagagtgtga ccctgctgat taaaggacat aacgcttttg
     9001 gttatctaaa atcagaaaaa ggtgttcatc gccttgtacg gatttcgcct tttgactcag
     9061 cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga
     9121 tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag
     9181 gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa
     9241 cagtagtagc tagtcaggca caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga
     9301 tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac
     9361 ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc
     9421 cctattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta
     9481 tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac
     9541 ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc
     9601 tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt
     9661 aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca
     9721 aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt
     9781 gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat
     9841 agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga
     9901 ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat
     9961 cggcagaaaa cctcgagata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt
    10021 gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc
    10081 tattgctcgg gcaatcgtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa
    10141 tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg
    10201 aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt
    10261 aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga
    10321 tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct
    10381 ggatgacagt tgcttcagtc agtgcggtaa cgatcacgtt gactttagtc ggtgtattca
    10441 tggcagttat catgaatgca actaaattag ctcaggatat agaaggaaac gttgatgtat
    10501 ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga
    10561 atgacttaga tcatgttaaa aaagttgaat tttcaagtaa agaacaggaa ttgaaaaaaa
    10621 tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg
    10681 atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag
    10741 cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc
    10801 aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag
    10861 cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc
    10921 aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag
    10981 gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg
    11041 catggttttt ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc
    11101 cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt
    11161 cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata
    11221 aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga
    11281 aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga
    11341 ggaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg
    11401 aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg
    11461 gcatggctgc tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta
    11521 ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag
    11581 aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct
    11641 actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc
    11701 tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg
    11761 attgaagata tcaagcaagc agaagaccat atccacttcc agtattatat ttatcgcagt
    11821 gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa
    11881 gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat
    11941 ttgaaaaaag caggcggaga aatcgcattt ttctttcctc tatttgttcc gtatattaat
    12001 cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat
    12061 acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg
    12121 cgagacaatc atttgcgtat ttacggagaa gcggtgtata tcttgcaaaa ccgttttttg
    12181 atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc
    12241 attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat
    12301 gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc
    12361 caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt
    12421 tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg
    12481 gcaacgtatt ctttcgctgc agagctgatc gagtatggag caaggatcga aacatatgag
    12541 aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca
    12601 gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact gatttatgat
    12661 gaacgaatgg cttcacgtgt aagaaaagca ttttttgaag actcgaaaat ttctacgcat
    12721 ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca
    12781 agattgattt cgccgctcct ttaaaacaca aaaaggacat gagaaccatc aacagatgga
    12841 gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg
    12901 tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata
    12961 agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta
    13021 atgagatagt tttttttaca attatgagga tttattttgt tatttaatga ttttgattcg
    13081 ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta
    13141 atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt
    13201 tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct
    13261 ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta
    13321 caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga
    13381 aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat
    13441 cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc
    13501 agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt
    13561 ggaaaagatc agccagtagt tatattgaac cgtgcttcag gaagcggaac ccgtagcacg
    13621 tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc
    13681 ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct
    13741 tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat
    13801 gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca
    13861 aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc
    13921 gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga
    13981 aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt
    14041 tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag
    14101 ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa
    14161 atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta
    14221 ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc
    14281 aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg
    14341 aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct
    14401 gctattgttg ccacaccatt tgccattggt gcagcagtat tcatgacgga gatctctcct
    14461 aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc
    14521 gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg
    14581 acagggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc
    14641 acaacgatga ctgttgatgc attaaaggca gtaccacggc actatcggga agcttcatta
    14701 gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc
    14761 gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc
    14821 caaatggtca tcggtaatgc agcattgatg ccaacaggac ttactactcc tgcttctaca
    14881 ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat
    14941 gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc
    15001 cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg
    15061 ctacaggtgt tttatatgta gtttcgggga tcatcgtatt gattttagca gctttactac
    15121 tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca
    15181 aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt
    15241 tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat
    15301 atgctggaaa aaactggctg acagatgtga ttcgcacgtc gattgaaatc ttgagctcat
    15361 tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg
    15421 gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta
    15481 gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca ggattggctt
    15541 taggtttgtc acgctgggaa acagtgataa aagtggtggt ttcagaagcg acaccaggga
    15601 ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct
    15661 atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt
    15721 caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca
    15781 atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga
    15841 tcattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa
    15901 aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa
    15961 tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg
    16021 gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac
    16081 tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat
    16141 gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat
    16201 taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg
    16261 tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg
    16321 cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt
    16381 gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca
    16441 gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga
    16501 gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt
    16561 gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcgg ctcgtatcag
    16621 cgactatact gcctttttct atctaggaaa agtaatggaa tatgacaaaa ctaacaagat
    16681 atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga
    16741 cggaggcctc aacatgacaa aagaaattat ttcatctaaa gatcttcatc tgtattatgg
    16801 aaagaaagaa gcactaaaag ggatcgatct aagtttcaat caaggggaaa tcactgcgat
    16861 gatcggtcct tctggttgcg ggaaatccac ttatctgcgt tctttgaacc ggatgaatga
    16921 cctgattccg aatgtaacga tcaccgggag tgttttatat aaagacaaag acatctatgg
    16981 tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa
    17041 cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa
    17101 aaataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga
    17161 tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg
    17221 tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac
    17281 aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga
    17341 gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa
    17401 gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat
    17461 gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa
    17521 cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga
    17581 aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga
    17641 taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg
    17701 cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg
    17761 tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt
    17821 gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa
    17881 agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta
    17941 tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga
    18001 ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt
    18061 catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt
    18121 gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac
    18181 gaaccacaat gagattttct aaactaaagc ggtgattctg ccgtttgttt aggaaaagag
    18241 gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac
    18301 aaaaatagct cctcatattt ttcatttggc tggacttatt accgcagatg caagcttttg
    18361 aacactgttt attcgggaaa gggtttgtga caaaatatgt cacaaaccct tttcatcatt
    18421 tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt ttttatttgt
    18481 gtgcttactt catgggttat tcagcatcaa gctgctttat atgaagtacc tgtcatcgag
    18541 atcgaaaaag tacgaataaa agaggatcat caattgatta caggaagact gatgaacacg
    18601 aatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca aatagaaaca
    18661 gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga atggcagcta
    18721 gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat tggcgccatg
    18781 ttgcttattg gcggaaaaca tggtctattt gctttaggaa gtctcatcat caatatcgtt
    18841 ttacttttac ttttgttgct catctttact aaaatagaag ggatttctct attaaatata
    18901 tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga tggatggaat
    18961 agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc attttttatt
    19021 tgtcttttga caatgaatct ttggaaagac caaggactgc gctatgagga actgaattat
    19081 ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac agctggagga
    19141 acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa gcgacaacca
    19201 gaaataacag cgaaacagtt atggctttcg ggaaggcaag tgggtcgaga tgtgattggc
    19261 tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc gcttctttta
    19321 tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc attggaattt
    19381 ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc ggtcctcttt
    19441 tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt gggattttag
    19501 caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta ggcttattca
    19561 tcaatttagg gatctttttc ttgttgttat tcggttatca tcagaagtgg ccgatactgg
    19621 cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt atcaacggtt
    19681 ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt tgtttcctac
    19741 tattgcttcc tatcactgat tttttagcaa tccaaggttt tacaacgata gaactagaag
    19801 aattatctgg cttagataag actttagcga ttgattttag attacttacg agatcgttgc
    19861 ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct agtgggacat
    19921 tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat tctagtttcg
    19981 ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca ttcatgggaa
    20041 gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag caagtgatca
    20101 atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg tccgcactac
    20161 ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag aaagaacaac
    20221 tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata
    20281 atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag
    20341 aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag
    20401 atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca
    20461 aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat aaacttgaaa
    20521 acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga caagatcaag
    20581 aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc
    20641 tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag aaacaggaag
    20701 cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac
    20761 agcgtcagac gttagaagca gaaatcaaag agttagaagc tagtggcgat acactgttag
    20821 aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa tggtctgaga
    20881 aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat caagcaacag
    20941 atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggaaaattct
    21001 tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa tggaaagatg
    21061 tcagcttgcg tgttcctggg attgcgacaa cgaaatttga gcatgaattt tactatgaag
    21121 ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt
    21181 gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa atgggtgctg
    21241 aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat catatctcct
    21301 tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct aaacgagtat
    21361 acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca ttggaagcta
    21421 aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta actgcaacga
    21481 tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt
    21541 ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa aacctttctg
    21601 tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag
    21661 aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc ggtttggaag
    21721 gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac gaagtagttc
    21781 gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa
    21841 tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat acagataaat
    21901 aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt gtaaaatagc
    21961 ttcccctatt ttacaaaatt gtgccttatt gccgataggc tatctcttga acaccgttta
    22021 ccaagagatt atgaacttgc caattatcgc cgagtcacaa ggaataattt caaaaaaagc
    22081 tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcaaaaa gacaccgatt
    22141 aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga ggtgagacat
    22201 gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt ttgacaggga ctttagcagg
    22261 aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg tattcctgag
    22321 cttagttgct tttggaagtc caatcatttt atacatttta ttagctttga ttatcccatc
    22381 tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa aaaacaatta
    22441 tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga
    22501 cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac gttaacattc
    22561 atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg gacggccgtt
    22621 cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt gacgatactg
    22681 tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa tgcagcgatt
    22741 cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc attcggtgca
    22801 gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt
    22861 agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta gaatcaggaa
    22921 tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg tagtactttt
    22981 ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag catgaatgtc
    23041 agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg tgacaggcga
    23101 cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag gactagagtt
    23161 gactggttat tttaattatt attcccataa tcgtttacag ctatttggca gtaaagaaat
    23221 cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa gattatgtag
    23281 tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag aaatgatcca
    23341 ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt ctcgcttatt
    23401 aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg tccacggtgt
    23461 tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga taggaaaaag
    23521 tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg atcgcagacg atcgagtaga
    23581 tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc tggaacatct
    23641 gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg cgagtgcggt
    23701 acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa aagataagaa
    23761 atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtag gcattccaca
    23821 aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag tagccgcgat
    23881 gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag aaagattaag
    23941 tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat gttagcacaa
    24001 atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc attgatcatc
    24061 gtatcaggta tcattatcgc catgtggctt agcagcagag aggctgttcg ggtaggatta
    24121 aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat tattggcgct
    24181 cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat ccaaatattc
    24241 aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc gatcacgctg
    24301 tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat cgctgcacca
    24361 agtgtattat tagcacaagc aatcggtcgt tggggaaatt tcatgaacca cgaagcatat
    24421 ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt catcattgat
    24481 aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc actttggagt
    24541 ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt gaaaaaaggt
    24601 gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat tgaaggatta
    24661 cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt atctgctgtg
    24721 ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa tttaccggat
    24781 tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg ttaaaaatga
    24841 aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac caggctcttg
    24901 gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga tttggggaca
    24961 tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt atttaccaga
    25021 tcattgtcta cccgattcca tccaagcatt caccaatatg gaagatgcag taaaagacgc
    25081 agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa aagaactagt
    25141 gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag aacaggggac
    25201 acataaaagg atctctgata tcttgttaga ggaaatccca gtagaaaaaa gaaaagctgt
    25261 tgttgttctt tctggaccaa gccatgcaga agaagtagcg aaacatgaca tcactacgat
    25321 cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt tcatgaataa
    25381 ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg cagctttgaa
    25441 aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg ataatgcaaa
    25501 ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag caatgggtgc
    25561 caacccgctg acctttatcg gattaagcgg agtaggcgat ctagtcgtta cttgcacaag
    25621 tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc ccctagaaga
    25681 ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag cagcgatgga
    25741 actagcccaa ataatggaaa tcgaaatgcc catcactcaa acaatctatg aagtgctgta
    25801 tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg ggaaaaaaga
    25861 aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt caaaaaagca
    25921 gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc tatggcaaag
    25981 gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga agcgttgaaa
    26041 tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat cgaagatcac
    26101 tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac cgaattattg
    26161 aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc tcatccaaag
    26221 ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt
    26281 atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt aatcgatgat
    26341 tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga tgaaacttca
    26401 aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa tgtcaaaaac
    26461 tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat tggacgttac
    26521 ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc cggaaatgaa
    26581 atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt tgcccgtaaa
    26641 ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac aagtattgaa
    26701 tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct gtctttaggt
    26761 aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat aacagaaatc
    26821 aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta attgaataaa
    26881 aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc cttatagaaa
    26941 agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat tgatcgcagc
    27001 aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag tttctaaaac
    27061 tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa tcgtcacaaa
    27121 agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta atgaactgct
    27181 cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag ttgctgattt
    27241 aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga aagtcggcgg
    27301 aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga cagcgatgaa
    27361 attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat ggctaaaaaa
    27421 ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc cgcgttattg
    27481 ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc ggatgatttg
    27541 atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga gttaacaggt
    27601 attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc aggtagtgtc
    27661 aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga tatcttgtta
    27721 aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc agaagatatg
    27781 aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga tatcatgatt
    27841 gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat gtcagaagca
    27901 aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga tgcgaaagaa
    27961 gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta aaatcacaaa
    28021 tttatagaat aa
//
LOCUS       DACIFS010000036        27219 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.036, whole genome shotgun sequence.
ACCESSION   DACIFS010000036 DACIFS010000000
VERSION     DACIFS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.036"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="IYA83_001599"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="IYA83_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ8587034.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="IYA83_001600"
     CDS             complement(2204..4390)
                     /locus_tag="IYA83_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ8587035.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IYA83_001601"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IYA83_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ8587036.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="IYA83_001602"
     CDS             complement(4612..5052)
                     /locus_tag="IYA83_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ8587037.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="IYA83_001603"
     CDS             complement(5126..5665)
                     /locus_tag="IYA83_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ8587038.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="IYA83_001604"
     CDS             complement(6280..7746)
                     /locus_tag="IYA83_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8587039.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="IYA83_001605"
     CDS             complement(8056..10137)
                     /gene="cadA"
                     /locus_tag="IYA83_001605"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ8587040.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10279..10458)
                     /locus_tag="IYA83_001606"
                     /pseudo
     CDS             complement(10279..10458)
                     /locus_tag="IYA83_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(10661..11020)
                     /locus_tag="IYA83_001607"
     CDS             complement(10661..11020)
                     /locus_tag="IYA83_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ8587041.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11050..11391)
                     /locus_tag="IYA83_001608"
     CDS             complement(11050..11391)
                     /locus_tag="IYA83_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587042.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11388..12062)
                     /locus_tag="IYA83_001609"
     CDS             complement(11388..12062)
                     /locus_tag="IYA83_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ8587043.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="IYA83_001610"
     CDS             complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="IYA83_001610"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ8587044.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14577..15767)
                     /locus_tag="IYA83_001611"
     CDS             complement(14577..15767)
                     /locus_tag="IYA83_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ8587045.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15788..16315)
                     /locus_tag="IYA83_001612"
     CDS             complement(15788..16315)
                     /locus_tag="IYA83_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8587046.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16362..19151)
                     /locus_tag="IYA83_001613"
     CDS             complement(16362..19151)
                     /locus_tag="IYA83_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ8587047.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEMELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19298..19498)
                     /locus_tag="IYA83_001614"
     CDS             complement(19298..19498)
                     /locus_tag="IYA83_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ8587048.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19950..20270)
                     /locus_tag="IYA83_001615"
     CDS             complement(19950..20270)
                     /locus_tag="IYA83_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587049.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20654..21772)
                     /locus_tag="IYA83_001616"
     CDS             complement(20654..21772)
                     /locus_tag="IYA83_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587050.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22243..23550
                     /locus_tag="IYA83_001617"
     CDS             22243..23550
                     /locus_tag="IYA83_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ8587051.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23669..24868
                     /locus_tag="IYA83_001618"
     CDS             23669..24868
                     /locus_tag="IYA83_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ8587052.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24895..26163)
                     /locus_tag="IYA83_001619"
     CDS             complement(24895..26163)
                     /locus_tag="IYA83_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ8587053.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26326..26973)
                     /locus_tag="IYA83_001620"
     CDS             complement(26326..26973)
                     /locus_tag="IYA83_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ8587054.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         7670 a         5847 c         4764 g         8938 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    10261 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
    10321 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    10381 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    10441 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
    10501 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    10561 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
    10621 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    10681 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    10741 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    10801 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
    10861 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    10921 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    10981 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    11041 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
    11101 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    11161 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    11221 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    11281 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    11341 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
    11401 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
    11461 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    11521 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    11581 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    11641 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    11701 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    11761 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    11821 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    11881 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    11941 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    12001 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    12061 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    12121 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
    12181 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    12241 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
    12301 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
    12361 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    12421 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    12481 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    12541 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    12601 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    12661 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
    12721 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
    12781 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
    12841 ggagctgaac ggcaggcgca caacctcttg atataaacgg tgttcaatca gctgaccctc
    12901 gatattagct aaaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat
    12961 actcaggtca aggcactgat ttcacaacct cttccttaaa cggtgttcaa aagccagttt
    13021 ctcggtatta agccaaattt atgcaaaaat gtggaaagcc atttccacaa aatttttctt
    13081 aatactcgaa actaaacagc tttttcacaa cctctccact aaacggtatt caaaagctta
    13141 catctgcggt aataagtccc gccaattcaa aaacatgaac agctgttttt gcttggctgt
    13201 tctaattact tgatgcagaa cagctttttc atcacctctt atggataaat tcggttcaac
    13261 aaacgtggga atggaatagc ttcacgcaca tggtcgatac cagcaatcca agtgactgtt
    13321 cgttcaaggc ctagaccaaa accagagtgc ggaacagaac cgtatttacg tagatcaaga
    13381 taccatgaat attctttttc atccaaaccg tgattacgga tttgttctag caagaaatca
    13441 tagtccgttg cccgttcgct tccgccaata atttcaccgt aaccttctgg tgcgatcata
    13501 tctgcacaaa ttaccacgtc ttctcttgtt ggatgtggtt tcatgtagaa tggcttgata
    13561 gcttttggat agtttaggat aaagacaggc tgttcgaatg aattcgcaat gaacgtttca
    13621 tgaggtgaac caaagtcttc gccccattcg atgtcatcaa atccattttt cttcagtaat
    13681 tcgatcgcat catcataagt aatacgaggg aatggcaatt ttgtataagt ttttaagact
    13741 tctttgtctc gtcctagtac atctaatgcg tattcgcaat gatctaaaac attttgtact
    13801 aagaatgcta catattgttc ctgtacttct aaactttctt cttgatgagt gaaagccatt
    13861 tctggttcga tcatccagaa ttcaatcaaa tgacgtcgtg tttttgattt ttcagcacgg
    13921 aaagtcggac cgaatgaaaa gacttttccg aaagccatag cagcagcttc catgtataat
    13981 tggccacttt gagataaata agctttttga tcaaagtaat ttgtttcgaa tagatccgta
    14041 gtgccttctg gtgcggaacc agtcaagatc ggcggatcga ttttgacaaa atgatgttga
    14101 ttaaagaatt catacgttgc acgaatgatt tcatttctga tctgcataat cgcatgttgt
    14161 cttgaagaac gtagccataa atgacgatga tccatcaaga agtctgttcc gtgttctttt
    14221 ggtgtgatcg gataatcgtg gctctcgctt accacttcca catttgtaat tccgatttca
    14281 taaccaaatt ttgaacggct gtcttcacgg atttctccag ttactaacac ggatgtttct
    14341 tggtgcagat ttttagctgt ttggaaaact tcttccggca cttcgctttt tacaactaca
    14401 ccttggaaaa aggctgttcc gtctcttaat tgtaaaaatg cgatttttcc gcttgatcgc
    14461 ttatttgcga cccaagcgcc gattgttact gtttctccca catggttttt cgaatcgatg
    14521 atttgaattt gttccacgta tgactctcct tttatattcc ttgaatttct tcaggattaa
    14581 ttttgacttt tactctcaat aaattgcttg attctgcgta ccgcttcttc taacgtagcg
    14641 cgatcagtgg cataactcat gcggacattt tctggtgccc caaagccttc gccagtcaca
    14701 agcgctaccc ctgtttcttc taaaagatct tctacccatt gtgtgacatt ttcatattta
    14761 cacatgcgta gcgtttcttt tacattagga aaaagataaa aagctccttg tggcttttca
    14821 agagaaacac ccggcagttc tgccacaagc ggatataacg tattcaggcg ttcttcaaag
    14881 gcgttccgca tttcttccac tgtatcttgc tcgcctttca atgcttcaac agctgcatat
    14941 tgactgactg ctgtcggatt gctagttgat tgggaagcaa cagcgatcat tccattgatg
    15001 atcacaggat ttcccaccgc ataaccaatg cgccagcctg tcatcgcata tgttttagac
    15061 acgccgttga tgatgatggt ctgatttttg attgcttctg aaatcgttgc aattggagta
    15121 aactcattgc cattataaac taaacggcca taaatatcat cagcaactat caaaatatca
    15181 ttttttactg cccattcacc gattgcctgc agttcattct tgctataaat cattccagtc
    15241 ggattcgatg gagaattgat gattacggct tttgtttttt ctgaacgtgc ttgttctagc
    15301 tgctcaacag taactttcca gctattggat tcttctcctt tcaaaaagac aggaagtcct
    15361 tctgccagtt ttacttgctc accgtaactg acccagtaag gcacaggaat gatgacttca
    15421 tcttgcgggt caaggatcgt ttggaaaagg gtatacaaag caaatttcgc tccgtctgtt
    15481 acgactgttt gtgaaggttc atagacaagt ccatagtttt tcttcagata atcaacgatt
    15541 gcctgtcgaa gctctgggat tcctgctgtt ggtgtataat agctagcttt cccattgcgg
    15601 attgcttcga ttgctgcttc ttggatgttt tctggtgtcg caaaatccgg ttctcccaca
    15661 gtcaaactca gcacatctct gcctttggct tttaatgcct tggcctttgc cgcagctgcc
    15721 aaagtgacag acggttctaa tcgttttgtt ctttttgata actccatccc attcattcct
    15781 ttacctttta aattcccttg attgtcttga cttcatctcc agattcaaag ccaatcaggt
    15841 aataattcag tttcccattt gtttcttttc ctactacttc ccacacaggc tgatcatcat
    15901 acatccctaa agcagctttt tcgatttgga tttcaggatg ggccgcagca attgtctgtt
    15961 ttgcttcatt ttcagtcagt ccctttgttt gatccaatac ttttactttt tcaccagatt
    16021 taggaatgat aacggcgatt tcttttcctt tatcatttgt acctgtcagt gaaaagtatg
    16081 tcttctttcg agtaaaccaa taaaaatgat cggcttcctc aagatttgca tatttctttg
    16141 cgatttctac tgcttctttc ttcgcctgca ccgccggacg actggaacgc aagtaaaaaa
    16201 tcgagctgat cacgatgacc gctaataaaa atacgatcaa gccaagtaac acgcgattaa
    16261 tcgttttttc ctttgtctgt tttttcttca agggtcgtcg ctcctttatc ttcattcctt
    16321 ttcattatag cagattttaa ttatgaaaaa agacagctcc tctaacttcc ttcttcattt
    16381 ttctttaaga attcatggat atcctgatag atttcctcca tagctagctc tttgatcggt
    16441 acttttttcg gtaatgcatg gatgatccgt gcaccatatt ttgcagtaac tagtcttcga
    16501 tccaaaataa gcaatacgcc acgatctgtc tccgtcctta tcaagcgtcc taatgcttgg
    16561 cgtaacttca aagctgcttt aggtacggat tcctggtaaa aaggattgat cccctctgat
    16621 tctaaataag cgttcctagc tttgaccatc gggcgaagcg gattatcgaa gggtaaacgt
    16681 gtgactacaa cgatctgcaa agcatctcca ggcaaatcta caccttccca aaaactgtca
    16741 gcgccaaaca atacgctttt ttctgacaaa atgaagcgtt tcaaaagctt ctcccggctt
    16801 ccgccaaacc cttgagctag tatttctcgt cctttttcta gcatagacag atgcattttc
    16861 tgatagacct tctgcaaaac atcatgagaa gtgaataaca caagtattgg ccgatcttct
    16921 tgttcgatca gacctgtaag tgctgtctct aaataatcga tatatgcttg attgctgact
    16981 tgatggacgg caggtgcctc ttttggcaca tagatccttg cctgtttctc ataatcataa
    17041 ggagaggcaa tcactttaag aggtgctgct tcaatgccaa gccgcttaga gagatagtgt
    17101 cgattagggc cgatcttcag cgttcctcca atataaagaa tacgatcata tcgaggatac
    17161 caaacagtcg acggcaacat agccgcttga aaatcatgga gatgaaaaat catctgctgc
    17221 gctgtgttcc caggaacgaa ccaatggatc aaatgatctc gccagtcttc cagccattga
    17281 tccatcagca agtactgctt gtctaattga tcaaataagc tatataaact tgctaaatcg
    17341 atccgcatct ggtttgtcca gctttcttgg ataatctcaa aagtcagacg catttgtgac
    17401 tgtagctgct ggatctcctg ataaaaaagc tgcaggtgat caatggctgt cccagattcc
    17461 agtggcagct gatccaacat ctcttttgtc gtcatgactt cttctggata ctgtccgtta
    17521 ggttgcttgc cgctcactaa actcattaat ccttctgcca tacaaatctg tgcttcgcat
    17581 agcgcagaaa gttcttctcg ataaatcgat aaccagcgta aggcttcatg ttctttcggt
    17641 aaatagcttt gtattcggtc aaacagctga tttggttctt ctaattgctg gatttgttta
    17701 tggaaatagt taatacttaa tcgtttgtca tttacttttt cagcgatttc cggcaaatga
    17761 tgtacttcat cgatcaacaa aaatggacta gctggaagca atggttcttt tcgctgtgtt
    17821 tcttgtatta aaaacgcatg attgacaatc aaaaaattac tttgcctgat tttcttttgc
    17881 aagaaacgaa caaagtcttg ttcgtaaaat gactgatttc ttgctaaaaa tccagtgcct
    17941 ctgtgcgcga tatctgcaaa gaatgaatgg ttcaaatttg ttaaatgtaa ttcatccaaa
    18001 tcaccggtct tcgtttgcgt cagccaaact aagaccgcca tttgatacaa agcatattgt
    18061 ttttgttctg gcggttccac aagtgtgcct ttaaaacgct gtaaatcaat atagtggcga
    18121 tagcttttga taattacagg ttgtatgggc tgatctagca attgattcag caatggaatg
    18181 tctttttcta tgatctgctg ctgcaaaact agcgaaacgg tactgatgac agcgggtttt
    18241 tcaggtgtag ccaaataact caatggcagc agataaccga ttgttttacc cattcctgta
    18301 gctgcttcga tcatcaaatc ttttgattca tcttttgtga aatggtcgta gaccgcattc
    18361 atcagtctgt tctgttcttt tcgatacatc agtgttttac caaacatttt ttcttttgct
    18421 tgtttttttc ttggatatgt ccgctcgcca taatggacgt atatgaatag ttccatttct
    18481 tttttctgca aagctagccc atcgacgatt tctaatgagg gatcaagtgg ctctggtttt
    18541 tctttcattt cttcaatcac atgataaatg aaacgactcg tatccatacc tgtgtgtcta
    18601 cttaatcgcg caatctgctc taacgtgatg attggcaatt ttctcatccg ttcttcgatc
    18661 agcaaaagaa gttgacccgt cacttcggca tcactatctg cctgatgagg attctcatga
    18721 ataaatccaa ggctttctga caaatcagct aagcgaaagc ttggttccgt cggcaagaaa
    18781 atttgtgcaa gttctactgt atcgatgcct ggtatcttca aactaggaag tccgcatcgc
    18841 atgagttcat ggttcaaaaa attataatca aaataaatat tatgcgcgac aaatatggtg
    18901 tctgccaaaa gattgtaaat cgtttgggcg acatcttcga aataaggcgc tttttggacg
    18961 cgttgattcg tgatccctgt taaatgctgg atttgttttg aaatcttccg ccctggatga
    19021 atatcgatcg aaaaacgtga agtgatccga cctgattcaa tcatgacgca gccaaattgg
    19081 atgatccgat cttcttttgg attcgtccca gtcgtttcga tatcaacgac tgcataagtt
    19141 cttggtttca aaatatcttc ctcttctcct gcctctaatg ttcttttgaa attatactac
    19201 aaagtgcaga aaaatactct catcgctttt taaagaaaat agacaaaaac gcccagacac
    19261 gcaaatgtct gggcgtttat tttttagtat aggttaatta agctttagta acgtttgttg
    19321 cttgaggtcc gcgttggcct tcttcaacat cgaaagttac tgcttggcct tcttctaaag
    19381 ttttgaatcc gtcgccttgg atagctgaga aatgaacgaa tacatcgttt ccgttttctg
    19441 cagtgatgaa tccaaatcct ttttctgagt taaaccattt tactgtacct gtttccataa
    19501 taaaaatcct ccttgtgtat caacacatat tttgcaatta cttgaatggt gtatatctgg
    19561 aagatgttta tttgaaacaa gctaccacag attaccgaac aaaaactact cctatagtat
    19621 atcataccgt ttacaaaaaa gctagagttt ttcgactttt tttattaaaa aaagttttct
    19681 cagcgattac ataaagtgaa aaaccgaact ttttttaaaa taaacgacat attgttcagt
    19741 tggtgttttt ttgctctgat gttcttcttt aaactaaaaa aattattctt tctgactact
    19801 tatcggttct gtttcaacgg tcgaggctgg ggaagattct ttagtaggtc tgatgttctc
    19861 atggtatttc tgatagccta tgacagttac cagaagaaca aggcaaacca atacgatcaa
    19921 cgagattagt ttttcttttt tgctcatatc tatacctcat aaataacagg atgatgtcca
    19981 tcatatttct gagtatgagt aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag
    20041 caactttcat aatcacaggt cggacagtac caaaagtaac caaatcttgg ctgcaaagac
    20101 cgttcttctg tttcaggaat cgcagaatca agatctgttg tctgtacata aacatttttg
    20161 tacagacgtg tgcctgtaat aggagaccgc tgtgtttctt cagcaaaaac tggctgactt
    20221 gttaaactta gggatagaaa cagcaaacac cataacagtc tttttttcat aaagacctcc
    20281 ttatcgataa gtttttccca attaaaatca tatctcaaga acgcccgtta ttcacatttt
    20341 gttcataaat gatgtttttt gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa
    20401 cctctttttt aaacggtgtt ccagaagtag cttcttcgga aataagtcga aatttcacaa
    20461 aaatttgaga aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc
    20521 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtattagc tcaaaaaacg
    20581 gaaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat
    20641 ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt
    20701 tttgatccaa aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca
    20761 acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg
    20821 acgtagcgat aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta
    20881 actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga
    20941 gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag
    21001 aagcagaaat cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg
    21061 tctgattttc tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat
    21121 ccttcaagta cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat
    21181 cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag
    21241 agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg
    21301 ccccttctgg tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg
    21361 agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag
    21421 ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat
    21481 ggatataggt ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa
    21541 acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg
    21601 aatatttttt ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat
    21661 ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt
    21721 tagcgccttc tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac
    21781 ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc
    21841 atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt
    21901 ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat
    21961 gaaatgctga taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga
    22021 ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg
    22081 cggaaacaca gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc
    22141 gcgttcttat cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat
    22201 tttaatttgt agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca
    22261 cgtctagtcc tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt
    22321 gctgtgcaag ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg
    22381 caaagcggta agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc
    22441 acaaaaatca tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg
    22501 gatgataaag tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg
    22561 aatgttcctt tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc
    22621 atccagtcag ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct
    22681 aaattcactg aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc
    22741 gttaatgcat caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga
    22801 gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc
    22861 ttggatttcc cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat
    22921 acgcaatcac cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac
    22981 aaggaaggcg tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt
    23041 ggtacgatca ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat
    23101 gcggaaaacc cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac
    23161 aattggtcag aaaaagagct aggaaaagct aacgcaagta ttcctagtct aaaaaccatt
    23221 gatgtaaaag acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta
    23281 tgggtacgaa acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag
    23341 caagtcactg acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct
    23401 acgatcacct tggcagacga taaattaggc tatttagaag acaacaatgc accttcaaca
    23461 gagatcatta cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc
    23521 gtcgcagaat ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg
    23581 gaaagatgtt tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta
    23641 tactgtataa gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt
    23701 tctctatctt attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc
    23761 cttgttcttt tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt
    23821 ggaaattttt tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc
    23881 tttttcttat ttcacagaaa aacgaaggct gctgaatggt cttttattca atgttttcct
    23941 catcagtttc ggcatctact tgtttgtctt gctgtatgaa acgcaaaata ttttccttgg
    24001 cggactgatc gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg
    24061 attgattgtt tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt
    24121 agctaatctt ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt
    24181 tttcttgtta aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat
    24241 cttgatctat ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt
    24301 caaccggcct cgatacaatc aagacttcat cgttgtgtta ggcgctgggt tgatcaatgg
    24361 agaaacagtt tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc
    24421 tcaaagccgt gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga
    24481 cgaaaaagta ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga
    24541 aagagacatt cttgtcgaaa ccaattcgac aaccactttg gagaacatgc tctattcaaa
    24601 ggaaatcatg gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta
    24661 ccacattttt agagccggtc tttatgcgtg gcaagccaaa ttgaaagcaa atggtattgg
    24721 tgcaaaaaca gctttctact acttgcctaa tgcgttttta agagaataca tcgccatctt
    24781 gatgatgaac aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt
    24841 cttatcgctg attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac
    24901 cttgtttttt tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc
    24961 tacttgctga gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat
    25021 cataaagaag tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc
    25081 aatcggtgta tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg
    25141 aacagaaaca tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa
    25201 tggtgtcact tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt
    25261 cccaataaaa tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact
    25321 caatccgaga taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc
    25381 tatcgtattt cgccaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac
    25441 gatcgaacgg ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt
    25501 gaagaacagc aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac
    25561 accatagctt tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc
    25621 ttctgttcgg actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg
    25681 tgtgccaaat acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc
    25741 tgatttatcc aatcggaaca aacgtgctgt attatctgct aacgctcccc ccatttggac
    25801 tgctgttcct tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag
    25861 tgtaagtgga ataagtcgca acgggatttt ttcttctcca ccatttcctt gatcgatcac
    25921 taagttgttt ccacgactag aaagaccgcc gtgatacgca tagagataag caaaaagcgc
    25981 accactaact ggtagtagat aaaggagcca aggatgtgat agtcggatat ctgtcacaac
    26041 tgtcaggcta tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt
    26101 gatcaccagc cacttcagca tgtacactaa aatgcgtact ggactcggta attgtttttc
    26161 cataaaaaat tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact
    26221 ttagtaggcg tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc
    26281 acttccatta aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt
    26341 tacacgttca gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc
    26401 actatcatga caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt
    26461 tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt
    26521 tatcgttggt gataaagtac cactaatccc ttttaatttt tcatcttcgt aatagatcat
    26581 gaattttttc tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat
    26641 ttcattccaa gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg
    26701 tttgctaggc tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctcgttctgc
    26761 ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga tttctggcag
    26821 ttcgtgttct aatatgtctc gaatcttttc agttgctagt gcctgaactg tttgtaccgt
    26881 ttttggatca ttgacagatg agtacgcatt taataactgt tctacttctc tttttataaa
    26941 aaaatattgg tatggtttta ttgtttgttc catgatttta aagtcctttc ttctaaccaa
    27001 agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct gaagatcccg
    27061 gactctttta aataagatac gttttgcatc ttatcatcac cccctttaat gcttattctt
    27121 ctatgtacat gattcattcg ataaataaaa gtaaaccgta tcttgaacag atagtcaact
    27181 gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaa
//
LOCUS       DACIFS010000037        26928 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.037, whole genome shotgun sequence.
ACCESSION   DACIFS010000037 DACIFS010000000
VERSION     DACIFS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.037"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(39..1145)
                     /locus_tag="IYA83_001621"
     CDS             complement(39..1145)
                     /locus_tag="IYA83_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ8587055.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1355..2356
                     /gene="ccpA"
                     /locus_tag="IYA83_001622"
     CDS             1355..2356
                     /gene="ccpA"
                     /locus_tag="IYA83_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ8587056.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2693..5038)
                     /locus_tag="IYA83_001623"
     CDS             complement(2693..5038)
                     /locus_tag="IYA83_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ8587057.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5317..6219
                     /locus_tag="IYA83_001624"
     CDS             5317..6219
                     /locus_tag="IYA83_001624"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ8587058.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6432..8207
                     /locus_tag="IYA83_001625"
     CDS             6432..8207
                     /locus_tag="IYA83_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ8587059.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8273..9859)
                     /locus_tag="IYA83_001626"
     CDS             complement(8273..9859)
                     /locus_tag="IYA83_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ8587060.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10110..10334
                     /locus_tag="IYA83_001627"
     CDS             10110..10334
                     /locus_tag="IYA83_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ8587061.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="IYA83_001628"
     CDS             10476..12227
                     /locus_tag="IYA83_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8587062.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="IYA83_001629"
     CDS             12227..14011
                     /locus_tag="IYA83_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587063.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /locus_tag="IYA83_001630"
                     /pseudo
     CDS             14337..15188
                     /locus_tag="IYA83_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            15242..16993
                     /locus_tag="IYA83_001631"
     CDS             15242..16993
                     /locus_tag="IYA83_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8587064.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16997..17167
                     /locus_tag="IYA83_001632"
     CDS             16997..17167
                     /locus_tag="IYA83_001632"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587065.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17207..18109)
                     /locus_tag="IYA83_001633"
     CDS             complement(17207..18109)
                     /locus_tag="IYA83_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ8587066.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18340..19743
                     /locus_tag="IYA83_001634"
     CDS             18340..19743
                     /locus_tag="IYA83_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ8587067.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19743..21176
                     /gene="ascB"
                     /locus_tag="IYA83_001635"
     CDS             19743..21176
                     /gene="ascB"
                     /locus_tag="IYA83_001635"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ8587068.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21321..22247
                     /gene="trxB"
                     /locus_tag="IYA83_001636"
     CDS             21321..22247
                     /gene="trxB"
                     /locus_tag="IYA83_001636"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ8587069.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22461..24188
                     /locus_tag="IYA83_001637"
     CDS             22461..24188
                     /locus_tag="IYA83_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ8587070.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24260..24553
                     /locus_tag="IYA83_001638"
     CDS             24260..24553
                     /locus_tag="IYA83_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ8587071.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24645..25505)
                     /locus_tag="IYA83_001639"
     CDS             complement(24645..25505)
                     /locus_tag="IYA83_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ8587072.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25597..26025
                     /locus_tag="IYA83_001640"
     CDS             25597..26025
                     /locus_tag="IYA83_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ8587073.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26187..26363
                     /locus_tag="IYA83_001641"
     CDS             26187..26363
                     /locus_tag="IYA83_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ8587074.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26390..26836
                     /locus_tag="IYA83_001642"
     CDS             26390..26836
                     /locus_tag="IYA83_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ8587075.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8570 a         4796 c         5691 g         7871 t
ORIGIN      
        1 aaaaggtggg agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat
       61 tcttttggtg tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg
      121 atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct
      181 tcttcgatta ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg
      241 atcccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt
      301 cgtgcgattt gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca
      361 agctgtgctt caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa
      421 gcaattgtac gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac
      481 acaagttcat tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt
      541 gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg
      601 tattcaattt ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca
      661 atctcaaatg caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga
      721 gttttgatca gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc
      781 gtcaagatat cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct
      841 acattgccag gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt
      901 tcgctgtcta aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact
      961 tccaaagcag gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca
     1021 cgctcatgag gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata
     1081 taagccaatt cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta
     1141 ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta
     1201 ttaaaagacc aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa
     1261 aacggttgca attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc
     1321 agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta
     1381 tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa
     1441 tccaaacgtt aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta
     1501 tcgtccaaac gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat
     1561 catccctgat gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc
     1621 aacaatgtac aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt
     1681 caatgtattg aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca
     1741 tatcacagac gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg
     1801 ctctattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa
     1861 agatgcagta aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc
     1921 gttgatcgat cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga
     1981 aaataaccta ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg
     2041 tctatctcta gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga
     2101 tgaattagcc atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga
     2161 attcgaaatc atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac
     2221 aagtatcaca caaccattat acgatttagg ggctgtatct atgcgcctat tgacgaaaat
     2281 gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa
     2341 aggatctaca aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc
     2401 tgcgttacaa ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg
     2461 tgattcggag ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt
     2521 gttataatcc tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg
     2581 tatataaaac aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg
     2641 gactgtgaca atactattgt cacaatccct tattactgtg tattctatta aatcaatcat
     2701 ctttctcatc tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc
     2761 agtaatctgt atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg
     2821 gtccattttt cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat
     2881 tgacagtaac ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat
     2941 cagatgataa ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt
     3001 gattgatcag attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag
     3061 tattgtcatc gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc
     3121 atgaatctcg gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat
     3181 ttccgtttaa gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac
     3241 tacgcatcat gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc
     3301 tttcgtgttt ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt
     3361 cttcatagac gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga
     3421 catctactgt acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca
     3481 tctttgccaa gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt
     3541 gatataagtg ataagccggg atattgttgg accattctag tgactcacgg acggtttgga
     3601 aggtgttcga tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt
     3661 gccaagttgt tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg
     3721 caggtccata aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg
     3781 catggttatt ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag
     3841 ttctgttatc catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca
     3901 tgtatccata attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg
     3961 aatggattgt atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac
     4021 tgaccgatga atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc
     4081 ttgttgcttc attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt
     4141 gttgtggtag aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg
     4201 taatttggcc ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag
     4261 aaagatcttc ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg
     4321 gtaatccagc gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga
     4381 ttccttttgc agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg
     4441 gtgagacatt caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg
     4501 cagaaagaat gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct
     4561 gtttgactaa ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag
     4621 atactaatgc tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat
     4681 cctctgtgct gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg
     4741 tgcgcatcag atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg
     4801 acgtttctgt aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt
     4861 cttcgacgag ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca
     4921 aaagaagaac agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa
     4981 caaaccattt ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa
     5041 gggtcacctc aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac
     5101 agacaaggct gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga
     5161 tctttattat aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga
     5221 acagggcaaa gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt
     5281 gttagtaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
     5341 atgagcggaa agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg
     5401 acttttatga tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg
     5461 aatattgatg ccggaaaagc gtacatggca gaaaaaggtc taacaaatat cgtcgttcat
     5521 gccccttata tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc
     5581 caatttctga gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt
     5641 cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc
     5701 ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt
     5761 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
     5821 ttgaatgaaa aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat
     5881 attaaagaag acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg
     5941 cttaaagtac tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac
     6001 gccaatattg gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca
     6061 atgtttgaca atatttcgaa aatcttggaa actccctatg taggcaaaga taaaaagaaa
     6121 gcttacgcac catatggaaa agaaatcgcc atgttcaaag cgcaaacatt cgatccagaa
     6181 gtgtttcctg agttattagc agaagaagtg atactctaag attttctatc tgacagcagc
     6241 aaaaaaggac caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt
     6301 tgcagtcttt ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta
     6361 tactggtaga aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga
     6421 aggacgaaaa gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata
     6481 catactttat tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta
     6541 aagcaatcta tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg
     6601 ctcaaaatat tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag
     6661 gaatctccta cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt
     6721 tgttcggcat gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgattg
     6781 tgaagaacaa gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt
     6841 ttacacatga atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga
     6901 tcattatcgc ttgtttatta gctatttatt accgcaaata cggttctagc agaaaattgc
     6961 tgccatggtt tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt
     7021 ctatctggct tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc
     7081 tagggcaaga cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct
     7141 cgaaacggga atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg
     7201 tctatcaatg gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc
     7261 gttccagtgg agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga
     7321 acttgaataa agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag
     7381 ccgcatctcc tacattccat actgtttcta cccgtattga cgaattatat acgactcacg
     7441 cgtggactca aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc
     7501 gagatgtggt aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt
     7561 ggaaaaaagt caaccaagaa ttagaagaag cctatcacaa gggaacaatc aaaaagaaaa
     7621 aagagttcta tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat
     7681 tcgatttcct tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag
     7741 gaaattatag tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc
     7801 cgctaatgga gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa
     7861 aatcagcaaa agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc
     7921 tgattgcttc aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg
     7981 atcgtgccta ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg
     8041 tttttctttt cggtgtctca tggttttgtt cttatgcgcc agaaaaaaag gactattttt
     8101 taagtgtcta cactggtgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc
     8161 tagttttcag taaattaaat ctgaaaaaag ctttatcttc taaataaaaa gacccttggt
     8221 agctagattt ttaacctaac tatcaagggt ctttgtgtgg aaaaatcagt ttttattcga
     8281 tttcttctac cgtttcctgc aaaagctgtt catatttttt gtttccttta tataaatcac
     8341 caatcgtcca tgccaacagg agaaggacaa gaacaatgat tacataagcg atgtgatcag
     8401 ccaatgctag ttgagcggaa gtggcttgct cgccgaagaa actttcgatc tgtcctggta
     8461 atcctcgtaa gttcaagtaa gttagactaa tcacagagaa ccagccaaga agttttacga
     8521 tgaatgaatt tttaaatcgg ctacccatct ccgcttggct gttggtcatc atcagtaaag
     8581 gaatcatcga aaacggcaac gcaaaagcca agaacacctg ggattcattc attaagttat
     8641 tcaaagcggc atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata
     8701 caggaatcac agaaagcaat cgtgtgacca gtcttcttag ccaaatcggg atacgcatat
     8761 ggatgaatcc ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc
     8821 cagaagcaag aagtgctaca gcaaataaag tggacaagat accagatttt gctacagcaa
     8881 tcaaaatacc attgctcata gctgatggat cagataaagc ttcatacaga ccaaaaaagg
     8941 aaggatcttg gacagaaccg gttttgaaga cagcgacacc catgatcaat agcagtgcat
     9001 tgactagaaa agccattgac aactggatat tagaatccca agtagaaaaa cgtacagctt
     9061 gtgctacatc ttcttcgttt tggtgatcga tcttccgagt ttgtgaaact gctgaatgaa
     9121 gatataagtt atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag
     9181 tttcgccgcc gatgaccggt gagtctgaga atgtatgact gttagggata aagccagcaa
     9241 ataaactgtg ccaatctggg ttagacaagc caacttgata gacaaaaacg aataaaatga
     9301 cgaaaattaa gcagacaacc agtgcttcga tcttacgaaa cccgactttt gtcagtagta
     9361 agagaacgaa aacatctaat acagtaataa agacggcaat caccaatgga atatcaaaaa
     9421 gtaagtagag ggcgattgct gcaccaatga cttctgcaat atctgttgcc ataatggcta
     9481 attcagtcaa gatccataaa atgacaccta atgtcttact tgtgcgtgct cggattgcct
     9541 gtgccaaatc catttggctc acgattccta attttgctgc catatactgt aaaagcatag
     9601 caatcagact agacatcaaa ataactgaca tcaataaata ttggaaattt tgtcctccgg
     9661 tgatcgaagt agaccagtta cccgggtcca tatacccaac tgctactaac gcaccaggtc
     9721 cagagtaagc aagaagtgtt ttaaaaaaac ttttcccctt tggtacatca actgttccat
     9781 ttacttcagc taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt
     9841 tattggattg attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac
     9901 acctcaaaaa gaaaaaatta aagaaaaaga ttcggtacgc ctaatttttt ttcatggcgc
     9961 gtcttgctag atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat
    10021 gaaactaaag aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag
    10081 caattcggaa atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg
    10141 ctattattgc tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga
    10201 aagactattt agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt
    10261 caatggggca aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc
    10321 aagggaaaaa ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt
    10381 tttatttcgt aatttgttag actattcaga ttattcagca aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aaattaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag atcgccgata
    10621 aaaatttatc aataaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcgattgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc acgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcaaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaga cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg cgatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactctc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcattgcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tttgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcctcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcctt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttttt
    12781 gccgatttta ggaatcgtca tctggtatta ccagaaattc agttctcgta tttaccgaaa
    12841 tatgcgggaa aaattaagtc agctgaacac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacgaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac ggaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gcggaaaaag ctcgatcatc aacgtattga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc aaaaatgcgt caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtaga attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt ctcatatttt tcatctgtga gagttagtgt
    14101 cgtagtcacc agatttaaaa aaagattcga cagctagtca gaagtgccta atagccacta
    14161 agaaagcgtt gacaaaagca tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctgat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatacatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa
    14401 tgatcgtgat gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact
    14461 catgcatcga caaaaaattt cagttgacaa atgacgggct ttcacaacag ttctaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt
    14641 atacggctat cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata
    14701 tcaaacaact atttcctaaa gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagatg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttactata acaatacagg
    14881 atattctaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccatt
    14941 tttttcgatt cgtcacacac ttacgttttt ttctgacacg cgtcctagat ggagaaaaat
    15001 cagaagtggg agaagtttct gaggaattgt ttcaggttat tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttagccga gacgttcaac taccagatgt
    15121 ccacggacga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
    15241 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
    15301 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
    15361 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
    15421 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg
    15481 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
    15541 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
    15601 tttccagcca ttccttgcta tcctagctgc tggcgggatg attcgtggac tgacagcatt
    15661 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
    15721 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
    15781 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
    15841 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
    15901 ttttgaagca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
    15961 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
    16021 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
    16081 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
    16141 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
    16201 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
    16261 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
    16321 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
    16381 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
    16441 gtttacatta ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
    16501 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
    16561 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
    16621 gatcgccatt gcgatggtgg tatcctttac gctgactttt ttcttttgga aggatgaagc
    16681 aggtgaaaca gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca
    16741 aggacaaatc gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg
    16801 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
    16861 catgaccctg ttcccgacga aacaggcaat tggaatggtt tctgaaacag ggctggaatt
    16921 gctgattcat gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt
    16981 gcagcaagga taaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat
    17041 cgaaaaggaa ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt
    17101 ggatattttg gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact
    17161 gacctgaacg gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt
    17221 tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt
    17281 tttgaccgac agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac
    17341 tatataacac gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg
    17401 aatacagtcg ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg
    17461 aatcaccgat actggtcaaa gctaaaattg gaacatgatt ttgtttcaat atttctgcgg
    17521 tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg
    17581 tgcaattgta cgcttcatag aatgattcac caaaagtggt gacaatcgtg acatccattt
    17641 tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga
    17701 aaatcacgat tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat
    17761 gaatgagaga aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga
    17821 tcgtcataat accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc
    17881 cttcaagata ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac
    17941 ccattttttt tgctacacgg atgagtgtag aggggtggac ataacatcgc tcagcaatcg
    18001 cttggatggt catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat
    18061 tttctgaagg agaaaaggag atttctttga ttttttcacg taataacatg tagatacctt
    18121 cttcttattt ttttacgtaa attataactt ttttgaaaat gatttgtaaa cggatttata
    18181 ctatataact taaaatagac attttttgac aagatattta caaaaatcca ctagaaaaca
    18241 gtaaattttg taaatgttac tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa
    18301 aaagataatg aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
    18361 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
    18421 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
    18481 cttgagatcg ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaag
    18541 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
    18601 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
    18661 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
    18721 gtcgttttgg caatcgttga ggacatgaag cgtttatgtc caaatgcttg gctgatcaat
    18781 ttcgcgaacc caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
    18841 gtgattggat tatgcaatgt gccagtgatg gcgatgatga cagaaccagc aatgcttggc
    18901 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa
    18961 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
    19021 gagaatggat taccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa
    19081 atgaacatga tcccttgtgg ctaccaccgc tattactatc gtgaagaaga aatgctggaa
    19141 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
    19201 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
    19261 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
    19321 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
    19381 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
    19441 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
    19501 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
    19561 aatccactta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
    19621 caccagcagt atttaccgca attcaaggag acaatcagcc gattaaaaga agaaggaatc
    19681 gaagttcaag atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta
    19741 taatgggatt cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg
    19801 gcggttatga tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag
    19861 accgttttcc tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt
    19921 atccagcaaa aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg
    19981 cagaaatggg gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg
    20041 gggatgagaa agagccgaat gaagctggct tgaaatttta tgaggatgcc tttaaagaat
    20101 gccaaaagca cggaattgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt
    20161 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
    20221 gtcgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggctgaca ttcaatgaga
    20281 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa aaaggagaaa
    20341 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
    20401 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
    20461 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
    20521 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
    20581 aaatggagcg aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag
    20641 aacatactgt tgattttatc tctttttctt attatgcttc ccgagtttcg actaccgaca
    20701 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc
    20761 aagcaagtga atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata
    20821 tttatgatcg ttatcaaaaa ccattgttta ttgtagaaaa cggcttaggt gcagtcgata
    20881 cgccagatga aaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata
    20941 tccaggcgat gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt
    21001 ggggatgtat tgatttagta tcagctggta ccggtgagat gaagaaaagg tacggattca
    21061 tttatgtcga tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat
    21121 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa
    21181 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
    21241 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
    21301 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
    21361 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg
    21421 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga
    21481 tcatgggccc cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa
    21541 atgcttatgg aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg
    21601 aagataaaag ttacgaagga aaaacagtca ttatcgcaac aggatgtatc caccgtaaat
    21661 taggcgtacc tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg
    21721 atggtgcgtt tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag
    21781 aagaagccat ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg
    21841 aattacgtgc acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta
    21901 tgtgggatac agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag
    21961 cacgtaatgt caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg
    22021 ttggactgga tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct
    22081 ggattccaac agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg
    22141 ttcgtgaaaa agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac
    22201 aacaagtgta taaatacttg gagtctttag aagaaacaac agcataagtt taaaaagagt
    22261 agaagaacgg gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg
    22321 atttatgcga acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt
    22381 tttggtcctc atactttcat atctaataag tgaatgctat acttattaag aaccatattt
    22441 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
    22501 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
    22561 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggtatgcgg ggaatcttag
    22621 gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac
    22681 gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
    22741 cacgccatca gtcaccagaa tttgcaatgg aagcagccaa aacactagca catcacaata
    22801 tcccgtctta tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc
    22861 atttgaagac gtttgcggga atcatggtaa ccgcatctca caacccagcg gcttacaatg
    22921 gatataaagt ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga
    22981 cgaaatatgt ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag
    23041 ctaaacatag cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag
    23101 aaatcaaagc agtgacgatt gatcgtgaat tggttgaaac aatggggaaa gatttgaaac
    23161 ttgtgtacac gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag
    23221 ctggattcga acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta
    23281 cggtcaaatc gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag
    23341 aaaaagaagg agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag
    23401 cggttcgttt gccagatggt tcttatgaag tattgactgg gaaccaaatc ggagcattgt
    23461 ttgctcaata tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg
    23521 tattgaaatc aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta
    23581 ccatgttcaa tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caatttgaag
    23641 aagaccattc acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac
    23701 cttttgttcg cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt
    23761 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
    23821 gctatttcgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
    23881 tctctgcttt gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag
    23941 ttattcaaac agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa
    24001 aactaacaac accgccatcc gacgtattga agtatacatt ggaagaaaat ggctggattg
    24061 ctatccgccc ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagtt
    24121 ctacagaaga cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag
    24181 aagaataaaa aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt
    24241 caagttggag gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca
    24301 attattcaaa atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg
    24361 ggaacaaaat gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca
    24421 tcaactacgt ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat
    24481 tttgtatagt cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt
    24541 ggaacatcaa taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaagaaatt
    24601 tcgttgtcac actccagtgt tttttaagaa aaagacaaaa atgatcaatc gcttttttct
    24661 gttgtttcag ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa
    24721 gccgtttctt caatagataa agatgctaca ttgatcacta cacatcctag tttttcataa
    24781 agatcattcg caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca
    24841 gggtttaatc cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta
    24901 tttgttagtc ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct
    24961 tgtgggatca aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa
    25021 ggcgttttgg aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga
    25081 ggatctttcc catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag
    25141 ttctcattca ataaatggag ggcaccaggt tgccgaattg gcttgatacc agttaaacgt
    25201 tcaatttctg atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa
    25261 aaagaacgtg ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt
    25321 tcttctaatg catcttgcaa tgcctgcctt aatgtttttt catctgtcac gaacgttctg
    25381 cggaataggt tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc
    25441 gcagtttctc cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa
    25501 gtcatggata aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc
    25561 gtacttcgta atcattttga tttggaggga taagtcatga aaaaacagac attgatcgaa
    25621 caatcattag aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg
    25681 ttaacttatt tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg
    25741 aatcaggaat tcaaaggggt aagttatgat acgatttacc ggaatctcca tgattttgca
    25801 gagatcgatt tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc
    25861 tgtcagggga aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa
    25921 gaaatccata tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa
    25981 gggcatagat tcgagatttt cggaagatgt gaaaattgtc aataaggatt taaataaaat
    26041 aacatttgat aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata
    26101 taatatttac aggacagtct tgttttttga gatatatttc aataacaatt acggttactt
    26161 tttaagctcg gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac
    26221 ttgacgatgc tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat
    26281 ctcgtaaacg tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag
    26341 ctagaaaacg caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact
    26401 aagtagatta aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca
    26461 agtcatccga atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt
    26521 gacagaagaa gaagaactaa cggtgttgtc tcgtgaaatg aaacagcgac gtgattcttt
    26581 gcatgagttt gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt
    26641 aatagtcgaa aaatatatgc cggaacaact ttccgacgaa gaaattcgac aacttgttca
    26701 agaagcaatc actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc
    26761 aattatgcct aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga
    26821 actattacaa tcttaatttt atcatttaaa ctgcggacag tgattatccg cagttttttg
    26881 tgttcgtaaa aaaagaacta aaaaacagct agaagaaaga atcttatt
//
LOCUS       DACIFS010000038        26238 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.038, whole genome shotgun sequence.
ACCESSION   DACIFS010000038 DACIFS010000000
VERSION     DACIFS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.038"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYA83_001643"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYA83_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ8587076.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IYA83_001644"
     CDS             1995..4661
                     /locus_tag="IYA83_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ8587077.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IYA83_001645"
     CDS             5025..5777
                     /locus_tag="IYA83_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8587078.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IYA83_001646"
     CDS             complement(5799..6953)
                     /locus_tag="IYA83_001646"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ8587079.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IYA83_001647"
     CDS             7138..9579
                     /locus_tag="IYA83_001647"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ8587080.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IYA83_001648"
     CDS             complement(9909..10613)
                     /locus_tag="IYA83_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587081.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IYA83_001649"
     CDS             complement(10800..11045)
                     /locus_tag="IYA83_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587082.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IYA83_001650"
     CDS             complement(11062..11913)
                     /locus_tag="IYA83_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ8587083.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IYA83_001651"
     CDS             12079..13083
                     /locus_tag="IYA83_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ8587084.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IYA83_001652"
     CDS             complement(13324..13680)
                     /locus_tag="IYA83_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ8587085.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IYA83_001653"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IYA83_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ8587086.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IYA83_001654"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IYA83_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ8587087.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IYA83_001655"
     CDS             complement(18040..18759)
                     /locus_tag="IYA83_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587088.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            18968..19600
                     /locus_tag="IYA83_001656"
     CDS             18968..19600
                     /locus_tag="IYA83_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ8587089.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IYA83_001657"
     CDS             19659..20519
                     /locus_tag="IYA83_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ8587090.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IYA83_001658"
     CDS             20626..23019
                     /locus_tag="IYA83_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ8587091.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IYA83_001659"
     CDS             23201..25042
                     /locus_tag="IYA83_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ8587092.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IYA83_001660"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IYA83_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ8587093.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8467 a         4668 c         5482 g         7621 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atctggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttagg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaa
//
LOCUS       DACIFS010000039        25502 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.039, whole genome shotgun sequence.
ACCESSION   DACIFS010000039 DACIFS010000000
VERSION     DACIFS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.039"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            133..3660
                     /locus_tag="IYA83_001661"
     CDS             133..3660
                     /locus_tag="IYA83_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ8587094.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IYA83_001662"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IYA83_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ8587095.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IYA83_001663"
     CDS             7448..7789
                     /locus_tag="IYA83_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587096.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IYA83_001664"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IYA83_001664"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ8587097.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IYA83_001665"
     CDS             9146..11566
                     /locus_tag="IYA83_001665"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ8587098.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYA83_001666"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IYA83_001666"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ8587099.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IYA83_001667"
     CDS             13000..13737
                     /locus_tag="IYA83_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587100.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IYA83_001668"
     CDS             13751..14566
                     /locus_tag="IYA83_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ8587101.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IYA83_001669"
     CDS             14579..15226
                     /locus_tag="IYA83_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ8587102.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IYA83_001670"
     CDS             15242..15898
                     /locus_tag="IYA83_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ8587103.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IYA83_001671"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IYA83_001671"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ8587104.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IYA83_001672"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IYA83_001672"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ8587105.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IYA83_001673"
     CDS             18240..18758
                     /locus_tag="IYA83_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ8587106.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IYA83_001674"
     CDS             18865..19365
                     /locus_tag="IYA83_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ8587107.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IYA83_001675"
     CDS             complement(19645..20565)
                     /locus_tag="IYA83_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ8587108.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IYA83_001676"
     CDS             20730..21743
                     /locus_tag="IYA83_001676"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ8587109.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IYA83_001677"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IYA83_001677"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ8587110.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IYA83_001678"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IYA83_001678"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ8587111.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IYA83_001679"
     CDS             complement(23383..24144)
                     /locus_tag="IYA83_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8587112.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IYA83_001680"
     CDS             24333..25427
                     /locus_tag="IYA83_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ8587113.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8447 a         4424 c         5384 g         7247 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DACIFS010000040        25305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.040, whole genome shotgun sequence.
ACCESSION   DACIFS010000040 DACIFS010000000
VERSION     DACIFS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.040"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(55..432)
                     /locus_tag="IYA83_001681"
     CDS             complement(55..432)
                     /locus_tag="IYA83_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587114.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            688..1416
                     /locus_tag="IYA83_001682"
     CDS             688..1416
                     /locus_tag="IYA83_001682"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ8587115.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1416..1670
                     /gene="purS"
                     /locus_tag="IYA83_001683"
     CDS             1416..1670
                     /gene="purS"
                     /locus_tag="IYA83_001683"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ8587116.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1672..2343
                     /gene="purQ"
                     /locus_tag="IYA83_001684"
     CDS             1672..2343
                     /gene="purQ"
                     /locus_tag="IYA83_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ8587117.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2344..4566
                     /gene="purL"
                     /locus_tag="IYA83_001685"
     CDS             2344..4566
                     /gene="purL"
                     /locus_tag="IYA83_001685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ8587118.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4551..5990
                     /locus_tag="IYA83_001686"
     CDS             4551..5990
                     /locus_tag="IYA83_001686"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ8587119.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6022..7065
                     /locus_tag="IYA83_001687"
     CDS             6022..7065
                     /locus_tag="IYA83_001687"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ8587120.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7062..7640
                     /gene="purN"
                     /locus_tag="IYA83_001688"
     CDS             7062..7640
                     /gene="purN"
                     /locus_tag="IYA83_001688"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ8587121.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7637..9172
                     /gene="purH"
                     /locus_tag="IYA83_001689"
     CDS             7637..9172
                     /gene="purH"
                     /locus_tag="IYA83_001689"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ8587122.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9188..10462
                     /gene="purD"
                     /locus_tag="IYA83_001690"
     CDS             9188..10462
                     /gene="purD"
                     /locus_tag="IYA83_001690"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ8587123.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10584..11498
                     /locus_tag="IYA83_001691"
     CDS             10584..11498
                     /locus_tag="IYA83_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ8587124.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11631..13196)
                     /locus_tag="IYA83_001692"
     CDS             complement(11631..13196)
                     /locus_tag="IYA83_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ8587125.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13189..14640)
                     /locus_tag="IYA83_001693"
     CDS             complement(13189..14640)
                     /locus_tag="IYA83_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ8587126.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14654..15670)
                     /locus_tag="IYA83_001694"
     CDS             complement(14654..15670)
                     /locus_tag="IYA83_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8587127.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15890..16978
                     /locus_tag="IYA83_001695"
     CDS             15890..16978
                     /locus_tag="IYA83_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ8587128.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17032..18558
                     /locus_tag="IYA83_001696"
     CDS             17032..18558
                     /locus_tag="IYA83_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587129.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18560..19765
                     /locus_tag="IYA83_001697"
     CDS             18560..19765
                     /locus_tag="IYA83_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8587130.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20022..20999
                     /gene="guaC"
                     /locus_tag="IYA83_001698"
     CDS             20022..20999
                     /gene="guaC"
                     /locus_tag="IYA83_001698"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ8587131.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21263..22687
                     /locus_tag="IYA83_001699"
     CDS             21263..22687
                     /locus_tag="IYA83_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ8587132.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23148..24257
                     /gene="pdhA"
                     /locus_tag="IYA83_001700"
     CDS             23148..24257
                     /gene="pdhA"
                     /locus_tag="IYA83_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ8587133.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24260..25237
                     /locus_tag="IYA83_001701"
     CDS             24260..25237
                     /locus_tag="IYA83_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ8587134.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8184 a         4309 c         5693 g         7119 t
ORIGIN      
        1 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
       61 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
      121 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
      181 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
      241 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
      301 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
      361 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
      421 tgcatcttcc atcggcaagt gcctcctcga acccattaac caaattttta gttaattcta
      481 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
      541 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
      601 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
      661 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
      721 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
      781 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
      841 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
      901 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
      961 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
     1021 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
     1081 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
     1141 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
     1201 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
     1261 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
     1321 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
     1381 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
     1441 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
     1501 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
     1561 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     1621 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     1681 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     1741 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     1801 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     1861 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     1921 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     1981 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     2041 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     2101 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     2161 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     2221 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     2281 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     2341 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     2401 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     2461 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     2521 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     2581 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     2641 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     2701 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     2761 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     2821 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     2881 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     2941 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     3001 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     3061 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     3121 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     3181 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     3241 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     3301 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     3361 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     3421 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     3481 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     3541 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     3601 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     3661 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     3721 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     3781 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     3841 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     3901 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     3961 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     4021 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     4081 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     4141 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     4201 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     4261 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
     4321 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
     4381 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
     4441 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
     4501 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
     4561 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
     4621 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
     4681 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
     4741 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
     4801 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
     4861 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
     4921 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
     4981 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
     5041 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
     5101 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
     5161 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
     5221 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
     5281 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
     5341 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
     5401 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
     5461 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
     5521 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
     5581 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
     5641 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
     5701 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
     5761 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
     5821 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
     5881 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
     5941 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
     6001 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
     6061 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
     6121 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
     6181 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
     6241 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
     6301 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
     6361 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
     6421 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
     6481 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
     6541 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
     6601 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
     6661 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
     6721 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
     6781 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
     6841 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
     6901 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
     6961 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
     7021 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
     7081 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
     7141 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
     7201 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
     7261 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
     7321 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
     7381 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
     7441 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
     7501 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
     7561 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
     7621 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
     7681 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
     7741 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
     7801 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
     7861 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
     7921 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
     7981 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
     8041 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
     8101 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
     8161 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
     8221 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
     8281 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
     8341 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
     8401 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
     8461 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
     8521 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
     8581 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
     8641 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
     8701 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
     8761 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
     8821 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
     8881 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
     8941 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
     9001 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
     9061 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
     9121 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
     9181 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
     9241 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
     9301 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
     9361 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
     9421 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
     9481 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
     9541 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
     9601 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
     9661 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
     9721 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
     9781 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
     9841 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
     9901 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
     9961 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    10021 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    10081 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    10141 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    10201 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    10261 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    10321 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    10381 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    10441 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    10501 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    10561 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    10621 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    10681 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    10741 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    10801 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    10861 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    10921 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    10981 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    11041 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    11101 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    11161 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    11221 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    11281 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    11341 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    11401 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    11461 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    11521 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    11581 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    11641 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    11701 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    11761 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    11821 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    11881 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    11941 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    12001 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    12061 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    12121 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    12181 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    12241 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    12301 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    12361 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    12421 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    12481 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    12541 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    12601 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    12661 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    12721 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    12781 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    12841 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    12901 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    12961 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    13021 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    13081 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    13141 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    13201 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    13261 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    13321 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    13381 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    13441 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    13501 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    13561 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    13621 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    13681 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    13741 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    13801 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    13861 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    13921 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    13981 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    14041 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    14101 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    14161 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    14221 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    14281 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    14341 tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc
    14401 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    14461 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    14521 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    14581 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    14641 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    14701 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    14761 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    14821 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    14881 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    14941 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    15001 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    15061 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    15121 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    15181 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    15241 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    15301 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    15361 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    15421 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    15481 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    15541 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    15601 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    15661 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    15721 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    15781 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    15841 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    15901 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    15961 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    16021 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    16081 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    16141 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    16201 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    16261 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    16321 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    16381 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    16441 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    16501 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    16561 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    16621 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    16681 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    16741 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    16801 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    16861 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    16921 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    16981 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    17041 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    17101 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    17161 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    17221 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    17281 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    17341 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    17401 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    17461 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    17521 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    17581 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    17641 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    17701 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    17761 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    17821 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    17881 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    17941 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    18001 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    18061 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    18121 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    18181 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    18241 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    18301 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    18361 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    18421 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    18481 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    18541 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    18601 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    18661 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    18721 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    18781 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    18841 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    18901 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    18961 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    19021 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    19081 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    19141 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    19201 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    19261 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    19321 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    19381 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    19441 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    19501 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    19561 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    19621 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    19681 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    19741 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    19801 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    19861 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    19921 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    19981 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    20041 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    20101 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    20161 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    20221 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    20281 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    20341 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    20401 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    20461 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    20521 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    20581 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    20641 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    20701 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    20761 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    20821 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    20881 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    20941 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    21001 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    21061 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    21121 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    21181 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    21241 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    21301 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    21361 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    21421 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    21481 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    21541 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    21601 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    21661 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    21721 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    21781 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    21841 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    21901 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    21961 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    22021 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    22081 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    22141 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    22201 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    22261 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    22321 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    22381 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    22441 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    22501 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    22561 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    22621 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac
    22681 agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag
    22741 ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    22801 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    22861 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    22921 tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    22981 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    23041 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    23101 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    23161 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    23221 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    23281 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    23341 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    23401 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    23461 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    23521 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    23581 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    23641 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    23701 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    23761 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    23821 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    23881 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    23941 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    24001 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    24061 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    24121 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    24181 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    24241 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    24301 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    24361 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    24421 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    24481 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    24541 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    24601 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    24661 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    24721 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    24781 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    24841 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    24901 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    24961 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    25021 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    25081 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    25141 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    25201 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    25261 catggcttat caatttaaat tgccggatat cggtgaagga atcgc
//
LOCUS       DACIFS010000041        24363 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.041, whole genome shotgun sequence.
ACCESSION   DACIFS010000041 DACIFS010000000
VERSION     DACIFS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.041"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(131..1426)
                     /gene="purB"
                     /locus_tag="IYA83_001702"
     CDS             complement(131..1426)
                     /gene="purB"
                     /locus_tag="IYA83_001702"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ8587135.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1439..2572)
                     /gene="purK"
                     /locus_tag="IYA83_001703"
     CDS             complement(1439..2572)
                     /gene="purK"
                     /locus_tag="IYA83_001703"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ8587136.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2556..3044)
                     /gene="purE"
                     /locus_tag="IYA83_001704"
     CDS             complement(2556..3044)
                     /gene="purE"
                     /locus_tag="IYA83_001704"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ8587137.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3460..4767)
                     /locus_tag="IYA83_001705"
     CDS             complement(3460..4767)
                     /locus_tag="IYA83_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ8587138.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4770..5351)
                     /locus_tag="IYA83_001706"
     CDS             complement(4770..5351)
                     /locus_tag="IYA83_001706"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ8587139.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5467..5564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5923..7215)
                     /locus_tag="IYA83_001707"
     CDS             complement(5923..7215)
                     /locus_tag="IYA83_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8587140.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7212..7835)
                     /locus_tag="IYA83_001708"
     CDS             complement(7212..7835)
                     /locus_tag="IYA83_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587141.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7838..8674)
                     /locus_tag="IYA83_001709"
     CDS             complement(7838..8674)
                     /locus_tag="IYA83_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8587142.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8691..9578)
                     /locus_tag="IYA83_001710"
     CDS             complement(8691..9578)
                     /locus_tag="IYA83_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8587143.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9594..10877)
                     /locus_tag="IYA83_001711"
     CDS             complement(9594..10877)
                     /locus_tag="IYA83_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8587144.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11560..12243)
                     /locus_tag="IYA83_001712"
     CDS             complement(11560..12243)
                     /locus_tag="IYA83_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ8587145.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12261..12878)
                     /locus_tag="IYA83_001713"
     CDS             complement(12261..12878)
                     /locus_tag="IYA83_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ8587146.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12888..13712)
                     /locus_tag="IYA83_001714"
     CDS             complement(12888..13712)
                     /locus_tag="IYA83_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ8587147.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13705..14265)
                     /locus_tag="IYA83_001715"
     CDS             complement(13705..14265)
                     /locus_tag="IYA83_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587148.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14439..15395
                     /locus_tag="IYA83_001716"
     CDS             14439..15395
                     /locus_tag="IYA83_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8587149.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15700..16656)
                     /locus_tag="IYA83_001717"
     CDS             complement(15700..16656)
                     /locus_tag="IYA83_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ8587150.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16760..17362
                     /locus_tag="IYA83_001718"
     CDS             16760..17362
                     /locus_tag="IYA83_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ8587151.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17450..18949)
                     /locus_tag="IYA83_001719"
     CDS             complement(17450..18949)
                     /locus_tag="IYA83_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ8587152.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18965..19489)
                     /locus_tag="IYA83_001720"
     CDS             complement(18965..19489)
                     /locus_tag="IYA83_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587153.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19787..20536
                     /locus_tag="IYA83_001721"
     CDS             19787..20536
                     /locus_tag="IYA83_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ8587154.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20527..21312
                     /locus_tag="IYA83_001722"
     CDS             20527..21312
                     /locus_tag="IYA83_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ8587155.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21281..21997
                     /locus_tag="IYA83_001723"
     CDS             21281..21997
                     /locus_tag="IYA83_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ8587156.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22099..23118)
                     /locus_tag="IYA83_001724"
     CDS             complement(22099..23118)
                     /locus_tag="IYA83_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ8587157.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23137..24195)
                     /locus_tag="IYA83_001725"
     CDS             complement(23137..24195)
                     /locus_tag="IYA83_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ8587158.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7233 a         5237 c         4238 g         7655 t
ORIGIN      
        1 ttaaaatcag gttcctatta ttgataagaa aatataataa actaaaagaa gcagaaccgc
       61 taacggatct gcttcttttt tggttatttt tgctattcta ttttaattga attttgcaac
      121 tcactcttct ttattccaaa cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat
      181 gatgataatc aaaggcatcg tctatttctt ctttagataa taccgatgtg atggttggat
      241 cttcttctaa cagcgggcgg aactgcactt ggttgtccca agcgtacgca gtttttggct
      301 gaacaagatc ataagcttct tctcttgtca ttcccttatc gatcaatttc agcataacgc
      361 gctggctata aatcaaacca aatgttgcag acatattccg cttcatgttt tcaggaaaga
      421 ccgttagatt tttaacgata ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg
      481 tatcaggcaa gatgattcgt tctgctgaag aatgagaaat atctctttca tgccataatg
      541 tgacattttc ataggcagtg accatgtgtc cgcgcaccac tctggctaga ccagccatat
      601 tttctgaacc gatcggattc cttttgtgcg gcatagcaga agaacctttt tgacctttcg
      661 caaaaaattc ttctacttca cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa
      721 aacgttcaat acttgtagca ataagcgcca tcgctgagaa atattcagca tgtagatccc
      781 gagggagaac ttgagtagaa atttcttgtg gacggatccc caattgttta catacatact
      841 cttcaataaa tggcgggata tttgcgaacg ttcccactgc tcctgagatt tttcctgctt
      901 ctactccttt tgccgcatgt tcaaatcgtt cgatgttccg tttcatttct gaataccatg
      961 tggcaagttt caatccaaaa gtagttggtt cagcatggac accgtgtgtc cgacccatca
     1021 tcactgtata tttgtgttct tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt
     1081 cttcccgtaa aatatcattt gcttgcttga tgagatagcc ataagcagta tctacgacat
     1141 ccgtacttgt tagaccataa tggacccatt ttctttcttc tcccaatgtt tcagaaaccg
     1201 ctcgtgtaaa cgcaacgaca tcatggcgtg ttaatttttc gatttccaag atccgatcga
     1261 tatcaaatga tgcatgctca cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt
     1321 ctgcccaagc ttcatctgct aaaatctcga cttcgagcca cgcacggtat ttgttctctt
     1381 ccgtccagat attccccatt tcagggcgtg tataacgttc taacatgttt tttctcctct
     1441 aatcccaaat attcgtttgt ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga
     1501 tagtgatatg tcccatcttg cgaccttttt ttgcttgttt ttttccatag aaatggaatt
     1561 gccagtctgg cttattctca atcaagcgat agctttccag cagttgttct cccaaaacat
     1621 tgaccattac tgcattgctt atcaaacgta ctttttctcc taatggccaa ccacaaatac
     1681 ctcgaatatg ggcatcgaac tgactcatag aacaagcttc aatagaataa tgtccagaat
     1741 tgtgaggacg aggagctaat tcattcacat atagcccgcc tgtttttgtt aaaaacattt
     1801 ctacccctaa aacacctgaa aggctcactg cttctgcaat cacacgggca atacgctcag
     1861 cctcctcgat cacatcctga tcaatcgcgg ctggagcaat cgtttcatga agaatattgt
     1921 tgtggtggat gttttccacg accggaaaag tcgtgaaatc tccttgacca tttcctgcca
     1981 ccatgatcga aatctctttt tcaaacggga tccatgcttc taaaacacag gttccttctc
     2041 ttaaaaggtc catggcagga gctagatccg cccgactttt caaaacatac tgccccttgc
     2101 catcatagcc gcctcgagtg gttttcaaaa cacaaggata gccgatcccg tcaatcgcat
     2161 cttggatatc cgtcggactg acgatcgttg cataaggagc aatcacgatg ttattcgttt
     2221 ccaaaaaaga tttttctaac aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag
     2281 gaataaatga catgggtaaa atcgcattta acgcctctac actgacgttt tcgaattcat
     2341 aggtaatgac atctgttctt ctggcaagct ctttcagcgc aaaggtgtca tcatagacac
     2401 tttcaatatg ccaatctgca acttgtgcgg ctggacaatc gctcacagga tccagcacac
     2461 ctactttaaa gcccatttct tttgcactaa tcgccatcat acgacctaat tgaccgccac
     2521 cgacgatccc gatcgttgaa ccaggcaaca aaggtttaac caagttgatc actactttct
     2581 ataacagttt tagccaacat tgaacgtctt tcagccaatt tagcctccaa ttctaaatca
     2641 tacatggaaa gcatctgtat cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt
     2701 gctgtagtag ctacaggtac tcctccaggc atttggacaa tcgaaagaag cgaatccaaa
     2761 ccatttaatg ttcgtgattg gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg
     2821 accatacccg gcagatgcgc ggctccccct gctcctgcaa taatgatttt gattcctttt
     2881 tttcgagcgt tttgtgcata ttcaaacatc aaatctgggg ttcgatgagc agaaacgacc
     2941 tttttttcat aagacacacc gaattcatcc aatatttgac atgcttcttt cattgtttcc
     3001 caatctgaag tactccccat aatcacagag atttttgctg tcattgtttt tcctcccagt
     3061 ttttctacat tcatatttta acaatcactt tttttgatgt caaagaaatt tcgaatgtta
     3121 acaaagaaaa acattttatt gttcgtgttt attaaagata aaaaaaagga atgtgacaaa
     3181 agttaggtca cattcctttt tatggataaa cggtgttctc ctgccaactc ctcggtcaca
     3241 aggaacaata ttcaacaaac atgagaagct tttttttgaa attgttcctt gtgactcgga
     3301 gatgaacggc aggctcacga cctcttcctt caacggtgtt caatcagctg accctcggta
     3361 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctgacaatc
     3421 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
     3481 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
     3541 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
     3601 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
     3661 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
     3721 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
     3781 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
     3841 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
     3901 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
     3961 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
     4021 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
     4081 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
     4141 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
     4201 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
     4261 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     4321 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     4381 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     4441 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     4501 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     4561 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     4621 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     4681 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     4741 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     4801 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     4861 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     4921 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     4981 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     5041 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     5101 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     5161 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     5221 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     5281 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     5341 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     5401 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     5461 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     5521 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     5581 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     5641 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     5701 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     5761 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     5821 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     5881 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     5941 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     6001 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     6061 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     6121 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     6181 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     6241 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     6301 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     6361 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     6421 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     6481 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     6541 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     6601 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     6661 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     6721 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     6781 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     6841 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     6901 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     6961 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     7021 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     7081 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     7141 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     7201 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     7261 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat gctgtataca ccagaacagt
     7321 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     7381 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     7441 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     7501 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     7561 tgaaacaacg taatgatgaa acacatccgt ttgatttttc ctttcctcca acataccttt
     7621 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     7681 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     7741 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     7801 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     7861 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     7921 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     7981 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     8041 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     8101 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     8161 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     8221 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     8281 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     8341 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     8401 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     8461 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     8521 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     8581 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     8641 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     8701 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     8761 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     8821 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     8881 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     8941 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     9001 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     9061 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     9121 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     9181 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     9241 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     9301 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     9361 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     9421 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     9481 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     9541 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttcctcctg ctgctatcct
     9601 agaatgtttg acgcaaactc ataaagactc tctgctgctt cttctgctgt tacttcttca
     9661 taaatgactt tttcaactaa aagattgtat tggtcttgaa tttccggatt caaagcacta
     9721 tcaaagacat tcacttcgcc gttgtccata tcggaaatca tttctaaata atcaaatgtc
     9781 gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc gaatattcga catgattggt
     9841 acaccacgtt ctccttttaa aatttcattg gcttcttcac tgttttgaaa gaaatcaata
     9901 aacttagcag cttcttcttt atgggctgag ctttttgcca tactcagcat ttgcgaagaa
     9961 cgtaaaggga taccagattc tccatctgct tttctgcgag gtggattaat caactgaatc
    10021 tccatatctt caggagcccc tgacaatatt tctgtcaact ggttacttga tagaaacacc
    10081 attgccgctt ctctatccct aaccgcctga aaagacagat ccgtcgtact tgcagctcca
    10141 ccacctggtt ctggatgggc ccctttgtct accagatttt tacgcagctg gaaaaaatct
    10201 gttagatagt ctggattttc aaatcccaag ctgttagata gatcttcccg attataaaaa
    10261 ttcaatcctt tctcaacttg tggtatttga ataatacagc cagctataaa atcttcgaat
    10321 cttgaaatag catagattcc taattcttca gtgatttgat agctgatttg ttcaaattca
    10381 tcccatgtcc aatttgaagc tggttcaggg atatttaatt gttcaaatat atttttattg
    10441 taagcaatcg caaacgaatt caccccgtta gacagtccaa ctaattgccc gtcgtaagtc
    10501 gtcgttgcta aataggattc atctgcatta gaaatatcaa ttatcccttg ctccacatat
    10561 tcatctaatg gttcaatctg attgatgtat tgaggaaatt gattgttcag ttggaaaatg
    10621 tcccagatat catctgatgc tgctagtgta cctaattttg tgtaatatcc ttcataatca
    10681 taaaattcat aatcgatcgt cacatgggga tttttttctt cataaagctc gattactttt
    10741 actgttgcat cgtgtcggac ttgcgaaccc caccaagcca tgttcagcgt gatttttttg
    10801 tcatcttctt gcccacaagc cgtcaagaag aataacataa cacttaaagc taagatgatt
    10861 ctcattcttt tcttcattct ctgcacctct acttccattg ttatttttca atatactgta
    10921 tttttataca gaacatattt attaaacgat ctttttcaag gggaataaaa accaagactt
    10981 gccttatcgt tctgcctagg atgaacaact caatactttc aagaaaaatt agatagtgct
    11041 gattcttctt tcatcgctaa aaacacttct tgcttcaatg atgggaggat aattacgcaa
    11101 agaaaaaagg atatcagaca actgcccaat atccttgaca tagctaaata agcatgcttc
    11161 ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag taattctgta tgaaattagg
    11221 tacaatatac acgtcgatgc acaagcattg actatggcaa cttgattggt cagttcccgc
    11281 tgacttttca agccccgaca atttagctgc tactaaattg tcgggtgcct tttttagttt
    11341 ttattcgttt ttcattctat agaaagcgct tcaataaatc aagacatttt tttgttagaa
    11401 tgctaataat aacaaaaggt tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa
    11461 aaatttctct tttttttgaa ttctgaacaa atagtactgg agtacaaaag acgaggaaga
    11521 gccactatct gctcttcctc gtcttttgat tctcacctgt tattcatgat tctttttctc
    11581 agtttcccga tatttttcca ttttacgact aaaaagctct ccgctacttg gtggcgtata
    11641 tgtgatggca tttgctcctg cttgaatggt ctcagcaata ctttcttccg ttggaccacc
    11701 tgtcgcaatg ataggaagtt caggatattt tttacgaaaa tggcgtactg cttctgcagt
    11761 ttctttacct gcactgatat tgataatcga aataccagct gctaactttt catctatttc
    11821 tgtatatttc gacgttaccg tactgataac tggaatatca actactcgac aaacttcttt
    11881 tactgtctct aaaggcgtag gaccgttcaa aacgacccct aacgaacctt gcgcttctgc
    11941 gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa
    12001 aactggaatg tcagctgcct caataatgct tttagtaatc gctggatgag gtgtaaaagg
    12061 atacacagca attactgcat tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa
    12121 gatgatcgaa cggatcaatt ttccaaatat cttgatcccg cttgcttcta aaatgatttc
    12181 cggtactctt acgatatctt ttctcaattc cgtcatgatt tctggcgtaa attgttctgc
    12241 catcagccac ttctcctttg ctatctccgg tcatactgat tcaattgata cgtctcgatg
    12301 acctggatga ttttttgcgc ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct
    12361 tgtgcggctt cctgatagtt ggtggccgta atcacttttt catacagcgc aggattccaa
    12421 tctactcctt ggacgaacag ttgtgtatgg tcatccatgg actgttccca tgaatcatac
    12481 actttgaagt ctccttggat agttatccat tcttcgttaa caaattcctt tgtttccagg
    12541 cttactttct tctgatcccc ataagccttg atcccgaaaa gattattgta tttgctagcc
    12601 aaggtacttt ttccccaatt tgattctaat atagcttgtc ctaaaatgat actaggaagc
    12661 actccataac catcttgtag ttcttgggca tgcggaacaa gccgttcaat aaattgctga
    12721 tgggattgcg cttcattttc ttgctgatta tcagtaaaag gagaagacag attcttcaag
    12781 gaaaaaacaa aggctaagag tatcactgct acaccggcaa ccactgctgg ccaacggata
    12841 ttcttgcgct tttgcttttt ccactttttt ttcgccatga tcaattccta cttttcattt
    12901 aattcattca gatattcttc tagcgtcaat tggtgtttgg tgatatactc agcgttttct
    12961 ttaccgacat aacgtaagtg ccaaggttca tatgtgatct tagttatatc ttcgtgtcct
    13021 tttggataac gaacgataaa tccatactgc gcagcattat ctgcgatcca tttagctcca
    13081 ggctgatctc cgtaacttgc atccaataca gtagaaggat aactgttgta ccagttttga
    13141 tcgacgacat cgactgccag acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt
    13201 tctttagtaa tctttgtcgc ctcctcttct gatacacctt gacgactcat cacatcttgg
    13261 acattttgtt cgaaaacttg ttgttgggat gatacagacc ggtaagctga gaccatcact
    13321 aatggaaatc cagctgcttc agcagccttg gcaaatgctt catattctgg agcaatgcgt
    13381 ttgtcgatca agtatccgtt acttaatgat gccaattgag tagactcatc aatttctttt
    13441 tctaatttgt gatctggacc aaccaaaact agcttccaat cgtctgtttt aacatctggc
    13501 aattgtttgg acgtacgatc ttttgtttct ttgtccttta ccttgctgct ggttgtttga
    13561 ctgttctttt ctttactagc agttgttttg gtgcttgttc gttcgactgc ttgattgccg
    13621 gcttgaacat gcgaatcatc atcgctaaaa ataacatagc ccattgctgc gatcataatc
    13681 acaactgctc caaatcctaa aaatttattc acttgaaaaa tcctttcgtt gttcttcacg
    13741 ttccccattc gcctcatgaa cgtaagtttc ttccagattt tcattcaccc aatcgagaag
    13801 ttcgattttt tctccttcga tctgtttttg aacatctgct ggcagacgaa aattcaaaat
    13861 atcatcatag ccccattcat catattgaat acttactttc aagtcaaggt atctaccagc
    13921 ctgctgatta tttttcaaag ggatcagctc gacttttgct gctttgctgc taagccattt
    13981 ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg
    14041 aaaaataatc gtttgacgca aaatcttttg cgttagcatc cattcataat cagtgaaaag
    14101 attttttact ttctgcatcg cttcagatgg cagatttttc tcgccatctt tccatttttg
    14161 ccattgttct ggactgatca gcaagtattc atctaaaaaa tttttttcag tttcaaactg
    14221 ttctaataat acactcataa taagatcaat atatacttga tgcacgttta tcccctcctt
    14281 ttaactattc tacttaagtt ttgatcaaaa agcacctctt aaatcgctgc tttcattttt
    14341 tttaatgttt caagtcctct cctctttatt cttcgtgatt tttgttataa taatagacag
    14401 acaagaaact caatccaact atgaaggagc gttttcagat gaaaaaatat caatggggaa
    14461 tcgttggtct tggtaccatc gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg
    14521 aactaacagc agttgcttcc agaacttcag aaaaagcgga aaacttcgct caccgttaca
    14581 atattccaaa agcttacgga agctatcaag aaatgttaga cgatgctgaa atcgacatcg
    14641 tgtacattgc tgtaccaaat agacagcata tcgaccatat cttggcagca ttggaagcag
    14701 gcaaacacgt tctatgtgaa aaagccatca cgatgaataa aaaagaactg gctgacgcta
    14761 tgcgcttagc agaagaaaag aatttgatct tagcagaagc gatgacaatc ttcaacatgc
    14821 cactttatca gcagttacgc tcaataatgg atacaggaaa actgggggct ttaaaaatga
    14881 tccaagcacc tttcggcagt tacaaagaac cagaccctaa aaaccggttc ttcaatccag
    14941 aattagctgg aggagctctg ttggatatcg gtacgtatgc tgtatctttc gctcgtttct
    15001 ttttgagctc tcaacctgaa gtagttgcct ctacaatggt tccttttgaa acaggcgtgg
    15061 acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga acttgctgct gtcagcttga
    15121 cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt tgagaacggt tatgtgacga
    15181 ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt tacagatggt acaaaggaat
    15241 tcatcgaaag cgggaataca tctgaagcaa tgaattacga aataaagaat atggtacaca
    15301 tgattgaagg tgacttgccc aatcgctctc tcttcttgac aaaagatgtc atcgaaatct
    15361 tggatcaaat gcaagttcta tggaaaaata tgtaatttta aaaggttgtg aaaaagctgt
    15421 tctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt tcgtttggct
    15481 ggacttatta ccgcagagga aagcttttga acaccgttta agaaagaggt tgtgagccta
    15541 ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgaat
    15601 attgtgactt gtgaccgagg agttgacagg agaacaccgt ttaaagaaga ggttgtgaaa
    15661 tcagcttttt gacctgaata ttagctgaat tcacaacctc tatttttgga ttttttgttg
    15721 ggagaattct gccttgattt caataaaact tttaaaagac tgcaaaaact ggaacaattc
    15781 tgctcgatcc tcgaattcag ggcgactagt cggcagaggc atttgccgat acaatacata
    15841 aacttcttca atacgtgtta aaatttgtct gccatcattt gactctgaaa atgtttctgc
    15901 agtataaatc aataaaccat agatatgttt tccataataa gctggttgct ggattctctc
    15961 tagattttcc agcatgtctt taatcacatt ggcctgtgct cttctcatag agaagtaagt
    16021 ttcgtagtat agcggctgtc tgagccagta gttttcttga tgttctctgg catcttcttg
    16081 gctttcgcga atgaatgtca gtaaatgttc gcatttgatc tgcacatctt ctttattttc
    16141 tgataaaaga aattctgcca tttgtctcaa aatatttcga aattcttttt cgatctgtat
    16201 ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa acatttgcaa gcaaagcaaa
    16261 tactaatcca atcgccatta atcccgcttc attgccgatc agctgccaag acatgttttc
    16321 ctccactaaa taatgagtga ccaaaaccga atttactact atcccttcgg ttaatttgaa
    16381 gcgagaggca agcgggatga acaggaataa aaaaagactg aagccaaaaa tagtataccc
    16441 aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat ataaaggctg cgattcgatt
    16501 catgcccgta cgcaatgtag agcgcttggt atttcctaca cttaataatg caatgatccc
    16561 tgcagctggc caatacaaca gcgataagct acccgctgtg accatcgaaa agaagacact
    16621 gatcactgtt ttgactgttc gcattcctat acgcatttac ttcccccttt tttattttaa
    16681 ttatacttga ataatgaaaa actagctatc agacaaaagt ttaggtatga tagtaaaaag
    16741 accaaaaagg agaatctcta tgcaatttgg ccgttttcga ctaggtatgc gtacgatcaa
    16801 aagtgcctta gctgtttttt tatgtatctt gttttttcat ttgactcaac gaggaatccc
    16861 gatgattgct gcattatctg ctgttttttc tttgcgtcag gacttgacta caaccgtttc
    16921 ttttggaaag tctaggatca tcggcaatac attaggtggt acattagcta tcatttattt
    16981 ttttgtcaaa agctattttc acaatgattt tttggtagaa ttgatcttgc ttcctattct
    17041 agttattatt gtgatcgtcg tgtcagatgg tattaacaat aattccggta tcatttccgc
    17101 aatagctacg ttgttgctga ttgcgttgag catccctcaa ggcgaatccg ctttgtatgc
    17161 tatccaacgt gtactagata cttttatagg gacattcatt gcaatcggta tcaatttctt
    17221 tcttcgtcct cctgaaacag aaaagaagga agaactatca gaagatttgg tcgaactcca
    17281 aaagaaagaa cgattgctaa gaacaaattt acaagagata gaagctaaga tccaaaaaca
    17341 aaagaaaaat agcgaggact aattttcttt tgcaataaaa taaaaaaatg gagagaagaa
    17401 ctttaaaaag tcttcctcca tttttgattg aacggtgaag tgtactactt tattcttcct
    17461 tcatcgagtt ttgttctgcc tcttttggcg cttcatacat atttacttgg ctagtagtcc
    17521 cacgtgtcga caaattctct acgatttcct tcaagtcaag tcctgttgtc gcttttagtg
    17581 tttcttgagt agaagaaagc aaatttgtgg cataatttgt cactcggttc gctccactgg
    17641 tttctcctga actgcttcct gtatcgacaa cagagatttt ttcgatattt cctagcggct
    17701 gtgctgcttc gcgcatcaat tgcggtagca tatcgataac catactcaag acagctgctt
    17761 cgccatattc cttgaaggca ttggcgatct tttgttttgc ttcggcttct gcttttcctt
    17821 ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg tgtcttattg gcttccgctt
    17881 ctgccagtgc ttccacacgg aatcgttctg cttcggcttc agtcacttct ttgacttttt
    17941 gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc tgcttttttc ttcacttccg
    18001 aatcatattg cttttcacga cgtgtgattt ctttttcttc cagttcgatt tgtttttgac
    18061 gttctacgat tttaatctgc atttcctgtt cgattacttg ttgttgtgct cgggcacttt
    18121 ctaaattgta ggcttggtct gcttttgctt tagcaacatc ttgttcttgt ttgtaggttg
    18181 ctaatttcag ttctttttct tttaatgatt ctgcgatctc tgtttgtcgc tgtaattcag
    18241 ctgcttgtga ttctttttct gcttgagctt ttttgatacg cgtttctttt agagcttccg
    18301 cttctgctat atctgcatct cttttgactt gggcgattct tggtttccct aaagagtcaa
    18361 gataaccgtt tttgtctctg acctctttga tagtgaaaga aacgatgatc aaccccattt
    18421 ttgcaaggtc tacagaagcg acttcttgta cgctttgact gaacttatcg cgattttgat
    18481 agatttcttc tacagtcata gaaccaagaa tcgagcgcaa gtgtccttct aatacttctc
    18541 ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa ctgttctgct gctgtagcaa
    18601 tctcttctac agatgaaccg attttgatga tcgaagtacc atcacacatc accggtacgc
    18661 cttgttccgt atacacttca ggggtggaga cgtccaattt actcgataat agactgatcc
    18721 gattcgaacg ttgaaaaact ggcaagacga atgcgccacc tccacgcaca attttgattt
    18781 tgttattgct ttcatcaatg tgcacatttt tgcttcctaa ataacttcca ctgatgatca
    18841 acgcttcatc cggttttgct gtctggtact tcgctacaaa gacgatcagc aacatcagta
    18901 aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg tatttccatt taacacacgc
    18961 tcctttaagc ttttatcatg ctttcataag gcatgacata acagattctt tccttgatct
    19021 caatgatcaa tactttcgtt ccccgctcag ctgtctgttc ttgggggctg taaaaacaag
    19081 ctggacgact aatggaaccg gtaacacttt tcaactgaat ttctcccata cctcgtacag
    19141 ggatcggtgt gatcacaaca gctacttgtc cttccagagt tttttcagaa gacgacatcg
    19201 ttgattcggc atgtttcatc ggcatcaata tatagaaatt aagcaaaaat accaacacta
    19261 tcgatatgcc tccactagca ataaataaaa ataaagatga ttgatctgtt aaacgctcac
    19321 ctagaaaacc aaataagctt gtgaaagtta accaagggat aataagcatc ggatcaagcg
    19381 ggccatcaaa atcgaataca tctccaaaaa taatcaatag gactgccaat cctgcacaaa
    19441 tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc ccctatcata tacacgcctc
    19501 cttccatttc tacgtctatc ataccaatgt ttgaaaattc tgtatagctt cttttaccga
    19561 ctcagtggtg ttttttatag gaaggttgtg tttatcgttg acaatttgtt aaatttttgt
    19621 tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc ctttttttaa
    19681 gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct ccacattcgt
    19741 ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga accgatctag
    19801 tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac tcatccaaaa
    19861 aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg aaatcatgat
    19921 tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat attctcacgc
    19981 agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg
    20041 aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt
    20101 ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa caaacaacac
    20161 gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga ttattggact
    20221 tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg
    20281 acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt
    20341 agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca atacacctgc
    20401 aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa aaaaatcagt
    20461 gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac cgttcggact
    20521 caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct ttttagcaat
    20581 gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat gaaattaatg
    20641 gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac ccatgaattc
    20701 atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga agaagtcacc
    20761 cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg
    20821 atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc caaagaacaa
    20881 tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat ggatgactcc
    20941 atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga agccgtgcta
    21001 caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac agaagctgaa
    21061 gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa
    21121 atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt gaatctcatc
    21181 cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac aaatggcggt
    21241 acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa atgtcctgat
    21301 aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa
    21361 tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa
    21421 aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta tccatttttt
    21481 atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg ctgcagatga
    21541 tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga tcaaaccgtt
    21601 cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc agacaatcca
    21661 aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca ccactcccat
    21721 tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa
    21781 aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac tggctcaaaa
    21841 aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag aacaagatat
    21901 attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt atgaattgac
    21961 aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat cccatacaaa
    22021 atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa cattttattg
    22081 acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata cctcattttt
    22141 cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga
    22201 taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag ggtacatttc
    22261 tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa
    22321 aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa
    22381 agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa tcaagatgct
    22441 accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt cacctgggat
    22501 atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa gcgagtggaa
    22561 cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca ctttggctcc
    22621 tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta tgatccctac
    22681 tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat ctttgaaaga
    22741 tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag tcaaagataa
    22801 ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac caaaatcagc
    22861 tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat aacgaatccc
    22921 atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc tcaacaatac
    22981 agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt cataaagact
    23041 gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg gacgaacacc
    23101 gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac ctatgccatg
    23161 aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa tagttgacag
    23221 taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc
    23281 cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt caaaattgac
    23341 tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg caggtacaaa
    23401 taccactttg actagactgt aataccatga agttttttca gatgcagctt ttttgaagct
    23461 tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta aaccagctaa
    23521 ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa acgctacttg
    23581 gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt ggaacagcca
    23641 aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg ctttgatatt
    23701 tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca aaaagattcg
    23761 gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa tcgcactgac
    23821 gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca acgttgtttg
    23881 cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa taatataaat
    23941 aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat tgatatcttg
    24001 catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt tcgatagaat
    24061 ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta ataatataat
    24121 aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg atcgaaaaat
    24181 gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat aaaaaaagct
    24241 tttattttgc taatacgtat taacattttt aattttatct ttacattgtt acttattgaa
    24301 caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttttact agaacacaaa
    24361 aaa
//
LOCUS       DACIFS010000042        23829 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.042, whole genome shotgun sequence.
ACCESSION   DACIFS010000042 DACIFS010000000
VERSION     DACIFS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.042"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(53..787)
                     /locus_tag="IYA83_001726"
     CDS             complement(53..787)
                     /locus_tag="IYA83_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ8587159.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1526
                     /locus_tag="IYA83_001727"
                     /pseudo
     CDS             1114..1526
                     /locus_tag="IYA83_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1519..2220
                     /locus_tag="IYA83_001728"
     CDS             1519..2220
                     /locus_tag="IYA83_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ8587160.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IYA83_001729"
     CDS             2220..3806
                     /locus_tag="IYA83_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ8587161.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISPMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IYA83_001730"
     CDS             3825..4046
                     /locus_tag="IYA83_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ8587162.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IYA83_001731"
     CDS             4043..4804
                     /locus_tag="IYA83_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ8587163.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IYA83_001732"
     CDS             5184..5693
                     /locus_tag="IYA83_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ8587164.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IYA83_001733"
     CDS             complement(5768..6307)
                     /locus_tag="IYA83_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ8587165.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYA83_001734"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IYA83_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ8587166.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IYA83_001735"
     CDS             7374..8171
                     /locus_tag="IYA83_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ8587167.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IYA83_001736"
     CDS             8199..8834
                     /locus_tag="IYA83_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ8587168.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IYA83_001737"
     CDS             8854..9627
                     /locus_tag="IYA83_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587169.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IYA83_001738"
     CDS             10035..10832
                     /locus_tag="IYA83_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ8587170.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IYA83_001739"
     CDS             10810..11223
                     /locus_tag="IYA83_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587171.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IYA83_001740"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IYA83_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ8587172.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IYA83_001741"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IYA83_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ8587173.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IYA83_001742"
     CDS             16000..16560
                     /locus_tag="IYA83_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ8587174.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYA83_001743"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IYA83_001743"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ8587175.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IYA83_001744"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IYA83_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ8587176.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IYA83_001745"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IYA83_001745"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ8587177.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IYA83_001746"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IYA83_001746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ8587178.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IYA83_001747"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IYA83_001747"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ8587179.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IYA83_001748"
     CDS             21907..22485
                     /locus_tag="IYA83_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ8587180.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IYA83_001749"
     CDS             22537..23019
                     /locus_tag="IYA83_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ8587181.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IYA83_001750"
     CDS             23136..23510
                     /locus_tag="IYA83_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587182.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5117 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct ccaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagtt
//
LOCUS       DACIFS010000043        22863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.043, whole genome shotgun sequence.
ACCESSION   DACIFS010000043 DACIFS010000000
VERSION     DACIFS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.043"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(153..551)
                     /locus_tag="IYA83_001751"
     CDS             complement(153..551)
                     /locus_tag="IYA83_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ8587183.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IYA83_001752"
     CDS             complement(548..1321)
                     /locus_tag="IYA83_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ8587184.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IYA83_001753"
     CDS             complement(1318..2001)
                     /locus_tag="IYA83_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ8587185.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IYA83_001754"
     CDS             complement(2168..3055)
                     /locus_tag="IYA83_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8587186.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IYA83_001755"
     CDS             complement(3052..3921)
                     /locus_tag="IYA83_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8587187.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IYA83_001756"
     CDS             complement(4058..5344)
                     /locus_tag="IYA83_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8587188.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IYA83_001757"
     CDS             5617..6195
                     /locus_tag="IYA83_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ8587189.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IYA83_001758"
     CDS             6195..7124
                     /locus_tag="IYA83_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587190.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IYA83_001759"
     CDS             7121..8857
                     /locus_tag="IYA83_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ8587191.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYA83_001760"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IYA83_001760"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ8587192.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IYA83_001761"
     CDS             complement(9753..10262)
                     /locus_tag="IYA83_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ8587193.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYA83_001762"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IYA83_001762"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ8587194.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IYA83_001763"
     CDS             complement(11563..12654)
                     /locus_tag="IYA83_001763"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ8587195.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYA83_001764"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IYA83_001764"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ8587196.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYA83_001765"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IYA83_001765"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ8587197.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYA83_001766"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IYA83_001766"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ8587198.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IYA83_001767"
     CDS             complement(16617..17591)
                     /locus_tag="IYA83_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ8587199.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IYA83_001768"
     CDS             complement(17572..19455)
                     /locus_tag="IYA83_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ8587200.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IYA83_001769"
     CDS             complement(19473..21698)
                     /locus_tag="IYA83_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ8587201.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IYA83_001770"
     CDS             21813..22847
                     /locus_tag="IYA83_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8587202.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         6563 a         4979 c         4121 g         7200 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaa
//
LOCUS       DACIFS010000044        21191 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.044, whole genome shotgun sequence.
ACCESSION   DACIFS010000044 DACIFS010000000
VERSION     DACIFS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.044"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..162
                     /locus_tag="IYA83_001771"
     CDS             <1..162
                     /locus_tag="IYA83_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ8587203.1"
                     /translation="NLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYS
                     SRKYINFDK"
     gene            323..1492
                     /locus_tag="IYA83_001772"
     CDS             323..1492
                     /locus_tag="IYA83_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ8587204.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
     gene            complement(1817..3136)
                     /locus_tag="IYA83_001773"
     CDS             complement(1817..3136)
                     /locus_tag="IYA83_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8587205.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(3133..3786)
                     /locus_tag="IYA83_001774"
     CDS             complement(3133..3786)
                     /locus_tag="IYA83_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8587206.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            4074..5007
                     /locus_tag="IYA83_001775"
                     /pseudo
     CDS             4074..5007
                     /locus_tag="IYA83_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4915..5631)
                     /locus_tag="IYA83_001776"
     CDS             complement(4915..5631)
                     /locus_tag="IYA83_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ8587207.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(5755..5940)
                     /locus_tag="IYA83_001777"
     CDS             complement(5755..5940)
                     /locus_tag="IYA83_001777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587208.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            6167..7440
                     /locus_tag="IYA83_001778"
                     /pseudo
     CDS             6167..7440
                     /locus_tag="IYA83_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7547..8578)
                     /locus_tag="IYA83_001779"
     CDS             complement(7547..8578)
                     /locus_tag="IYA83_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587209.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8585..9415)
                     /locus_tag="IYA83_001780"
     CDS             complement(8585..9415)
                     /locus_tag="IYA83_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587210.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(9412..10218)
                     /locus_tag="IYA83_001781"
     CDS             complement(9412..10218)
                     /locus_tag="IYA83_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8587211.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(10224..11048)
                     /locus_tag="IYA83_001782"
     CDS             complement(10224..11048)
                     /locus_tag="IYA83_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ8587212.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(11038..12138)
                     /locus_tag="IYA83_001783"
     CDS             complement(11038..12138)
                     /locus_tag="IYA83_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ8587213.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            12316..13101
                     /locus_tag="IYA83_001784"
     CDS             12316..13101
                     /locus_tag="IYA83_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ8587214.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            13134..13517
                     /locus_tag="IYA83_001785"
     CDS             13134..13517
                     /locus_tag="IYA83_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ8587215.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            13593..13724
                     /locus_tag="IYA83_001786"
     CDS             13593..13724
                     /locus_tag="IYA83_001786"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8587216.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            13693..13929
                     /locus_tag="IYA83_001787"
     CDS             13693..13929
                     /locus_tag="IYA83_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ8587217.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(14007..14978)
                     /locus_tag="IYA83_001788"
     CDS             complement(14007..14978)
                     /locus_tag="IYA83_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ8587218.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            15630..17252
                     /locus_tag="IYA83_001789"
     CDS             15630..17252
                     /locus_tag="IYA83_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAQ8587219.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRQFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(17538..18914)
                     /locus_tag="IYA83_001790"
     CDS             complement(17538..18914)
                     /locus_tag="IYA83_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587220.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(18914..19570)
                     /locus_tag="IYA83_001791"
     CDS             complement(18914..19570)
                     /locus_tag="IYA83_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587221.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(19580..20857)
                     /locus_tag="IYA83_001792"
     CDS             complement(19580..20857)
                     /locus_tag="IYA83_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587222.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(20876..21031)
                     /locus_tag="IYA83_001793"
                     /pseudo
     CDS             complement(20876..21031)
                     /locus_tag="IYA83_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT         6616 a         4309 c         3452 g         6814 t
ORIGIN      
        1 aatctaattg aacgactgaa tcaagaagta cgcagaagag aaaagattat tcgcatcttc
       61 cccaatcaaa catcagccaa tcgcttaatt ggagccgttc ttatggacct gcatgatgaa
      121 tggatttatt cttcaagaaa atacatcaat tttgataagt agaaattgta aaaacattgt
      181 atagcatttt acacaggagt ctggacttga ctcaaaaaga tagcgtaaaa tattttgtgt
      241 aaatagaaat ttagggtaga aaaaaaagtc cattctgtaa aattaaagtt accacacaga
      301 aattttatag gaggactttc tcatgactaa atttactata gaaattatgc aaacactact
      361 caataaaggg gatttagatg aattatttca ggatcattta gagcgagcga ttaattcact
      421 tctacaagca gagctaactg cttttttaga ttacgaaaaa tatgatcgaa atggattcaa
      481 ttcaggaaat tcccgtaacg ggaattattc acgtacattt aaaacagaat atggcgagtt
      541 gaatttaact attcctagag atcggaatgg cgaattttct caacaaacac taccagcata
      601 taagcgaacc aatgattcat tagaaacgac cattatccaa ttatttcaaa aaggcattac
      661 tatggctgaa atctccaaat taattgaaaa aatgtatggt caccactata cgccacaaac
      721 aatctccaac atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt
      781 agaagccaat tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac
      841 tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt
      901 tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga
      961 tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat
     1021 tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt
     1081 atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt
     1141 caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg actttatgac
     1201 taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc ctgccctatt
     1261 aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga acctgattga
     1321 aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga
     1381 atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat ttttaggtag
     1441 aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt aactgtaatt
     1501 aacagaatgg attttccatt tacacataat tcttgacgct accataatct aaataaccat
     1561 catattcatc tactacttat ataattgagt tttattaaac tttttaagtt tctattctat
     1621 caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt actaacaata
     1681 tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt tcactttcaa
     1741 taagaaaaaa gtctttactt ccatggataa taataagtct ctatttttca ataactattg
     1801 caaaattata tcttaattaa gaaaatattt ttattttaaa gataaaaaat tcttcatcct
     1861 gcaatacttt atattctata ttgtaattat tcagaatatt acttacaata tataaaccta
     1921 atccattact attttcctta tttaaatcaa atttaacatc aaatatttta tccatattcg
     1981 aaattttatt gttaccgtat gaattctcta tatataacca atcattaact atcccaatat
     2041 taattacccc atttacatca gtatatttta ccgcattact aatcaaatta gatagaataa
     2101 tctttaaagc tgtttttcct atataaacat tctcgtctaa tatataatta ttgacagtta
     2161 ttttcttttg atttgctaat atttcatact tttttaatat gtcttctaat gtatcattta
     2221 ttttcaaata ttcttcatca ttattcaaat tttctataga atgaactgat aatatttgag
     2281 aaatattttt tgttaaacta tcaacgattt caatacattc attaatataa atatctcttt
     2341 ctttatattt tccaatattg tatttcatgt tttccagtat tattttaaga ctagcaagag
     2401 gggttttaag ttcatgggat gcacctttaa aaaagtcata ttttaacttt tctaatttta
     2461 aaatttcttt atttttaaat tccaaatcgt caattgttct taataaagta gaatacaaat
     2521 cattaatttg ttgttttaat tctccaactt catcattaga gctaactttt aaactcattt
     2581 ttttatcaag tttcatcatt ttatcagtaa caatttttat ttcttgtata ttatttttta
     2641 ttgattttgc ataaattaaa gaaataatag cagaaaataa aattgatatt aataaagaat
     2701 atggtaaaaa tttaaggctt aaatctttcg catctttttg catatcagct gtagaaacaa
     2761 attgtagatt tgttttttta ccatcattaa gctttatttc tctttcttca attatcaaag
     2821 aattactatc actttctaaa ttaacattta tattatcttt gatttgtatt tcattcttat
     2881 tatttttttc ttttatgaat acttttattt cactactctt ggaataaaac tccaaagttt
     2941 gttctatata ttttatttct tttccattca tattactaga aatttcattt gccatattat
     3001 gaatcttttc ttttctcgtc tccaaatatg ttttaggaaa gataaaaaaa attaatgaat
     3061 gaactaatat aattattatt ccaagaacag aaaatatttg tatgaacatt tttggaaata
     3121 tctttaattt tttcattttc tctccaattt atatcctaca tttcttatag tagttataca
     3181 gtctaattgt aatttttttc taagttcttt tatatataca tctattactc gatcataagg
     3241 agtttcttcg ctatctttcc atacataatc aataatttgc attcttgtta acacttgtcc
     3301 attattatcc aataaacatt ttaatacttc tagttctttt gcatttacat ctatttcttc
     3361 accatttatt ttagctgtat agctattaaa attaaccgat agatccttat attcaaactt
     3421 ctctaaatgt ccataattct tcttaattaa agaatctatt ctagctttta agaccggcaa
     3481 tgaaaatggc ttttctacat atccgtctac taaattagta aatgcatcaa ttttatattc
     3541 ttcatcacta aatgcagtca agattagaat tggtaaattg cttttctttc taatttcttt
     3601 caacacttct aaaccattta taaaaggtat ctgaatgtct aagataacca aatttatatc
     3661 gctattaaat tttgacaagg cttctcttcc atctttagct tgaataacag tatatccaaa
     3721 ttctgaaaga tattcactta ttccctctct tatcatatta tcatcttcaa cagttaatat
     3781 tttcatatat acctccactt aagcctatac actagatatt atacagcata ttatggctct
     3841 ataatatcta gtgttggtga cgccttctgt tgccaatact ttttcttttt tttaataaat
     3901 gcaaacagac accttgaaat cctttagaaa aaaggcttct ataatatttt gtgttgttgg
     3961 aaaatcgaag gcgattttct actaacactt tttcttcgtt tcaactcaaa aagagactca
     4021 cttttggtaa aatttagtcg accaaaacat caccctacca aaggagtcgc tctatggata
     4081 atcatactag aaaattactc ggattaacag ataaacattt attttttgat gaagaatggc
     4141 tgattgaaaa agaatataaa ggcgttcaag cgcagtttat taaagggaga ttggatgact
     4201 caccttccca ttgtgaacac tgtggatcga ttcgtattat ccgcaacggt tcctatacga
     4261 cacgtactca gatgttgaaa gtcaaagaaa agttgactat tttagaatta aaacgtaccc
     4321 gttttctatg ctatgattgt gggcagacct tttctgcgaa aacggactta gttgatgaac
     4381 atcatcaact aacaaaggaa ctgaagcagg cgattctcat agaactgtac gaaaatcaat
     4441 cacgtaaact aatcgctaag aagtacttcg tctcggatgg gacggtgaca cggatcttgc
     4501 gtgaagcaac gaagcattac cagccacgta tgaacttcct tccgacggtg ttgtgtatgg
     4561 atgagttcaa atcaatgaag tctgtttcta gttccatgag ttttatttgt gttgatggga
     4621 caacccatca gctatttacg atccttgaag atcgcagact ttacaaacta actcaatact
     4681 tcctgcactt tcctagaaaa gcccgtttaa aggtcaagta cctagaatgc cagctattgt
     4741 cagctgctta aaacggtctt tccaaatgcg gagatcgtaa ctgatcgctt tcatattgtc
     4801 caacatatca atcgcagctt caatcaattg cgtatccaga tcatgaatcg ttttcgagcc
     4861 tcgcactctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa actcttactc
     4921 aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt taaacgacca
     4981 attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg aacgtacagc
     5041 gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat ccaaattaaa
     5101 agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga taccgccgat
     5161 cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat gaatattctc
     5221 cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac cagcttcaaa
     5281 tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta tcatttcacc
     5341 gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata atacaccgat
     5401 tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat ccaacgcttg
     5461 tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct caactacaac
     5521 agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat ctggtttgcc
     5581 gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca tcggatgtgc
     5641 ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat tttccagtgc
     5701 tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct tgacttatcc
     5761 cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct gcaaaaggaa
     5821 ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa tactctcgta
     5881 aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg ctttggtcat
     5941 cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct acagtatcag
     6001 cataaaaact gccgtctgaa aagtcaaagg tgaaagaaat gtggcgctat aatttctagg
     6061 actgatagta aatcattcta tcagtccttt tgagcataaa aaaacaaaac atacagaagc
     6121 tccgttagaa ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca
     6181 tcttatcaaa ctattactag gaatcaaaga tggaaacatt caaatcactc atgtcgagaa
     6241 gaaaaaaata cattcaatca acagtatcgt tgtttctgcc acgctcatta aaacgattca
     6301 gccctgtcct cagtgtaaac aggtgaatca acatgggctg atcgttaaga acggaaagaa
     6361 aaaatcgctg gtccttctca ataagtgtgc gaatcaacgg acttatcttg ctttggcaaa
     6421 gcaacggtac ctctgtcgtg gctgtcacac ctattttacg gcgaatacct acattgttga
     6481 aaaaaactgt tttatcgcca aacaagtaca ttataaaata ttggaggaat tgactgaaaa
     6541 acaagcaatg acgacgattt ctaaacattg cggcgtttct tggtcaaccg tttcgcgaac
     6601 actcgcttct ctgctcccca tgacaaacgt gaaaataaat tggttgcctc gctgttcatt
     6661 ggtagatgaa tttcgttcat tgaaaaatca agtcggtccc tattcttttt cttgtatgga
     6721 tggcgatact gggaaattac tggatattct tccttcacgt aagaagaaag atctcatttc
     6781 ttattttatg caatttgaac gacgtgcacg tttaaacgta aaaatactcg taacagatat
     6841 gaacgcctct tatgctttgc tgataaaaga gtgctttcct catgccaaac tcgttgtgga
     6901 tcgctttcac atcgtcaagc acctcattcg cagttttaag gatatttgac tatgagtcat
     6961 gaaaagcttc gaccgaaacg atccaataca agccaaacac tatcgacaag tgaaggcttt
     7021 atctcgactt ctgatcaaac gacaggacac gcttgtttat gataaatgga caaaatggcg
     7081 taattttggt tgggcctatt taaccgaaag tgaagtcgta gaacgtctac tttctacttc
     7141 tgatgaacta cgaatagctt atgcttacta tcaagaaatt cttcaagctt tttatgacaa
     7201 agaagcggac atcttctttc agcttgtaaa aacaatgccc aagtcggttc caagagaact
     7261 gcatcatata aaaaaagcgt ttataaacta tgaatccggc attcgtttag ccttagagtt
     7321 gccttactca aatggcaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgaat
     7381 agcttacggg ttaggatgag aaccagaatt ttcttaatca atcatctgat cacttattaa
     7441 acataaaaaa acatctaaag cagaaaatca attctacctt agatgttctt atatcactct
     7501 atcagtccta tttgacaaag agccagaaat gtttgccgac tatacactag ctttgttgtg
     7561 caaaatgctg agtatcaatt tccacaggga ttgctagaat atctttcagc tgaatgtata
     7621 aggcatcgct aattttctct tcccgatcgt tcaaccgatc cactaaaatt tctttgccct
     7681 ccagcgtttg aaaaacatac cgttgaatgt tgcctaaaat aaatgattcc ttccatagaa
     7741 ccggaatctt aaccccctga ggaaccggtt caaattgaat cagctccggt cttaaataat
     7801 aaatgcagcg attgccctta gcggaaaact cgtgggtata ctgattcagc tcataccatt
     7861 cgaaacgatt atagttgccg ataaattccg caacaaatcg gctctgagga ttctgataca
     7921 attccttcga ggtggaaacc tgagccaccc gcccagcttc catcacaaaa atccgatcac
     7981 tgattcgcat cgcctcttct tggtcatgag tcacaaaaat catcgtcatc cctagcgatt
     8041 gctggatagc gcgcaaatcc cgctgcagct gttttctaat tcgggcatcc aaagcactta
     8101 atggctcatc cagcagcagg actttcggtt cagtgaccaa tgagcgagcc agtgccaccc
     8161 gttgtcgttg accgccagaa agaaactgga catttttatc tgcctgctcc gtcatatcca
     8221 ccaatgatag catctcaaaa accttttcct gaatcacctc aggactcagc ttctttacct
     8281 tcaaaccaaa ggctacattt tcaaaaacag tcatagttgg aaacaatgca tactgttgaa
     8341 aaacaaagcc gatattgcga tccttcgtag gcttatcggc aatattctca ttgtccagca
     8401 caatctcacc gctctttggt tgttccaatc ccgtgataca gcgcagcaac gtagactttc
     8461 cgcacccgct gggacctagt aaagtaacca gctcaccctt ttcaatctga atttcatcaa
     8521 tagctaaaat ctgtttttca tcaaaaaaca gattgagatc atgtatttct aaaaacattt
     8581 tccgtcacaa ctcctttaag ggcttctttt tcgactgctt ctcttccctc gtctgcttaa
     8641 gggacagctg ttgcttttgt cgataagtca gcataaaggt agttaaagca attcccacca
     8701 taaacagcaa atacaaaacc atcacagcgc tagccaaatg gccgttttcg ttcatccgcc
     8761 gcagcatata gatgcgtacc gtctgaaatc gaccgccaat caacaggttc gtcagcatgt
     8821 actccccaaa aaccgaagaa aaaatcatca gagaaactag ggtcacaccg atacggatgt
     8881 ttggaaacag cacctggata ataatcgtag acatcggggc gcccaaggtt tctgctgcat
     8941 ccactaattc cttcagatta attaagcgca tctggttgtt aagactaaga tagacaatcg
     9001 gcaacgccgt aataaacaat gcacctacca acactacaaa cattgggata ccagtttcag
     9061 catacgttcg aagcaaagcc gttaccaaaa tcactcctgg taaagcatac ggcagcagga
     9121 gcagcttttc cagccaacga ttcaaacgag ggaaatacaa atgaatccaa ataatggtgg
     9181 gaatcatcaa caacagaatc gtcaccaaga ccacacctgc taataaaagt gagcgaccca
     9241 ccgcagcaat aaattccctg tccgtcagca gctgttggaa ccattgcaag gtaaaatcac
     9301 taggtaaaag cgattttgac catttagtgg agctggcata caaaatcgtt ccaataatcg
     9361 gcagcaagta atacaggaca aagcagaaca aaatcggttt tattaacttt ttcacgtctg
     9421 caccaccttg cctttctttt gaataatcaa cattgccgtt accagcagga cacctaaagc
     9481 gactgccaac gcactgccta aattaggctt ggcgaacaca tctcccgaca ctaaagccgc
     9541 aattcgaatc gttaaaagat ttacattact gctggtcagg gcatacgctg tttcataggt
     9601 ccccatgcca ttagcaaata agatgacaaa ggtgctggca atcgatggtc gcaaaaaagg
     9661 caaagccacc ttgaaccaag catacgctga tgaagcgccc agcaattgtg cggactccag
     9721 ccattccttt ttcacttcct tcatgctggg atacaaaaac aagaccccta acggaatttg
     9781 gaaataggta tacgtgatgg tcaatcccca ccaagaatac aaattgaagt ccgctagaaa
     9841 tggcaaaatc attttcagca cacctacatt ccccaacaaa ataataaaag agaatgccag
     9901 cggaatccca gcaaaattag ctgccaaatt ggaaacaaag gtgattttct cctgcaattt
     9961 agtcgataga ttcattaagc ataaacataa aataaacgtc ccgattaacc caatcacact
    10021 ggagctgaca gcaatcagta cgctgttaac aaaggcctgt gtgtaatatt tgttgctgaa
    10081 aatatcactg taattggcca atgtccactg gttagttaaa tcactgtgca agctgcacag
    10141 aaccataaaa agcagcggca gcagtaaaaa tgcacagaag ataaaggagg caggaaccag
    10201 aaccaagcgt tttttcatct ctttcatccc ctcatcaatt cttgaatgtc ctgcattttc
    10261 tctgacggtg caattcctaa taaccagcaa aacaatggag ccgccagcag ctgtggtaca
    10321 gtttgttcct tcagcttttg gcgtttattt gaaatcaaat aaaaaggaac ttcacgatgt
    10381 gcagccaagc tgccgccatg cagtccgtgc gcatccatcc cgtgatccgc agtcaccacg
    10441 acttgataac ctttttgcag ccattcagga aggtaaatag ccaataatct gtccgcctga
    10501 ttaaccgctg ctgcatattc atgagaatcg gcggtaaatt tgtgaccgac atcatcaata
    10561 ttcatcgaat gtacgaccaa aaagtctggc tgatactgat tcactaaata atgggcatcc
    10621 gcaaataaat gcgaatcagg gtaagtgtcc tcactataaa agatgccatg ctgaatcagt
    10681 cgctcctcct gatgctggat gcgatcaacc aaacgattat agggggcttg attgtacaac
    10741 tcgctgtacc agtggtaagc ggcggctcct gtttttccgc cagcttcctt aactaattca
    10801 aacagcgacc actctttgga tcgctgagca tggtaattac tatagatgcc attttcatag
    10861 gctggaacgc cggtcatcag tacttcgtat aacggacgtg aattgctggg cagctcactt
    10921 tttaccttca ataagcttgc ttgctggtgc tcaactaaat gattcatgaa cccaagctgg
    10981 gcgacggctg tgtcgtagcg acagccgtct agtataacta caatcagttt gtcaagtcta
    11041 ctttgcatag gtcagtacct ccgcctgcca aagctctgga agctctacag aggtcttgtt
    11101 ccaagcttca ttgtcttcaa ctggcaccgc cgcttcataa gcatcctccg gcagtaattt
    11161 ttcagctact tctttaggca gctctacctt gcgaataggt cgcgcatagc cctttgccaa
    11221 attgatttgc ccttcatcag ataaaatata ttcccgtgct agctttgccg cattgggatg
    11281 aggggcattt ttattgatta aggttgtata cccgctaatg atggagccgt ctgttggcac
    11341 agttacctca aaacgctcgg agtcaatttg gtcacgataa tttagtccat tgaagtccca
    11401 aacaactgcc acatcgattt cacctttttc aagatttgct acgctggcat caacggtgga
    11461 caaacggcct ttttctgcca tttttttgta gtaattcaga cctggctgaa tatccttttc
    11521 gtcacctccg ttggcaatcg ccgcagccaa tacagcaaat tgtgcttggt tggcaatcgt
    11581 cacatccccc acgctaactt gatagtcacc ctcttgcagc tccttccagc tagtcggtgc
    11641 ttgcttcaca ttctctttat cggtgataaa ggaaatggtt cccgtatagc ttaacaacca
    11701 atccccatca tcatcctttg cccaatccgg aatttcctcc caatagttgg tcttataagg
    11761 taaggttaac tctttgcttt cagccaacgg accgaagctg ataccgacat caccaatatc
    11821 ggcggttcca ttttttcctt ctgattcaaa cttagccaat tcttctgcac tggacatatc
    11881 cgtatccacg tgacttaagc cgtattcttt ttcaaggtcg ttccaagtgc ctacccaatt
    11941 tgcccaagta tccggcattc ccaagctggc aatctctcct tcttccttgg ctttttctat
    12001 aatcgtatct aaagagtcgt ctgccgcagc cgattgcttc gttgcttcac ctgagccgca
    12061 gcctgtcagc aggatactta ctgttgctgc acaaaataaa ccgatagtca tgttacgaat
    12121 tttttcattc attttcattc attcttctcc cctttcgata attccattct aacaaagctt
    12181 tgtaatgaga gaatcagttt cttgaaaagg tttcatgaac aatgtaaagg ttgtatatgg
    12241 tttgtaaatt tcgacgaatt cccgttaacc ttctaaagct tttgcggtat gatagtagaa
    12301 aaaaggaggc tccctatgca gtattatgat cagcatctcc acacgtattt ttcctttgat
    12361 tcagaggaaa aatttgaaaa ctatttagcc tatcagccgg agtttttcgt ttctacggat
    12421 cactttgatt taaacaatcc atctaccgga aaagatgaca ttccggatta tccagcctat
    12481 ataaacaaac tggactcttt aaaagaaacc tattccacca aatttctgcg aggtatcgag
    12541 attggcgttg ttccaggaca agaagagcgg atcgtggatt acctcgctca gcatccttat
    12601 gatttaaagc tggtaagtat tcatcaaaat ggccgctttg attatatgga cgatgtggtt
    12661 ttaaccaaag atcccattca aactaccaag gaatattttg atcagatgtt gcaggtcttg
    12721 acggattttc cagaaggaga tattttgact cactttgatt atggattacg ccgcttcacc
    12781 ttcacagcgg aagaattcgc ggagcatttt gaggctcagc tgctaacgat tttcaaaaag
    12841 gttgttgaac gagaattggc gatggagtta aatgccaaga gcttcttgaa atacggcaac
    12901 gaagaccttt accgttatgc cgttcctttg tatcaaagtg tcggcggaaa gctctttacc
    12961 ttaggctcgg atgctcatgt tgccgaagat tatcagctgg gctttaaaaa aatgagtcag
    13021 ctgttggcag actgtcaggt agaaaagttg ctagtcattc aaggaaaaga acgccgcctg
    13081 acgccgctgc cgattttgtg attcctatat aatagaagaa agtggtgaaa aaaatgagca
    13141 acctgtggga ctacaatcaa gaggcaccca tccattattt aattgcgcga cattgggatg
    13201 cgttgaaaat tgaggcggtt tgccgatcct tgttagcagc cgttccaaaa caacaactgg
    13261 aaaatttttt ggtggctgac agcttgcagc gggaaaaagt tcaggcttat tttgcagcct
    13321 ttaaagatca gcctttggaa tatttgcatg cgcagtttca tcttttttat caagtagccg
    13381 ccccagatga ctacaacgat ttacgaggac agctgcaatt gacctttcaa gcggatgaaa
    13441 ccgcctatac cgttttattg ggaatggctc gccttggtga tcaggcgaag gtcgagtggc
    13501 ggattttcga tatttgatgg caggatcaag gcgtttcagt tgcaaacctt cctctctttc
    13561 aattagaaaa accgcagcag gaagaagttt gtgtgctggt caccgatgct ggtgaaattc
    13621 gtctccgctt atttcctgac caagctcccc tggctgtagc caactggcgc tccctagctg
    13681 agcaaggcgt ttatgacaac acacctttcg gctcgtgtca ttaaggaatt tgttattcaa
    13741 ggtggcgctt tggatggttc tggtgatgaa gctgtttcca gctatgaggg ctttttcgct
    13801 gatgaagtgc atcgcggact ttatcatttt aatggcgcct tggccttagg ggaccacggc
    13861 ccccacacca acggcaacca gttttttatt gtgcaaaaca caaaggcgca ggctgacctg
    13921 ctaatgtaaa gcaagcatac ttggagcttg gaggactacc ggagctggat ggtcgttata
    13981 ctgtttttgg acaggtatac caagggttag aaatcgtagc agcgatcgca gctcagccga
    14041 caaacagtga agatgtaccg attgagccag tgttcattcg acaaattaac ttcgaaaaaa
    14101 catcgtaaaa agacactcac tgacttcagc aaaagctcta ttcttgagct ggctgcagct
    14161 agtgagtgtc tttcatttat ccataagata aagcttggat tcaataaata aataaatgtt
    14221 ttggttaaag ctgtgaccag caaatgagga accgtgcgag ccagttgatc tgccaatgca
    14281 aacatttctt taggtgaagg cgctggcgtg tctgccaacg gatactccag tcccaatgct
    14341 tggtaaccgt gcataccata gcagtggtac ggtagtaatt ctaacggtac gaaccgatca
    14401 agatctgcca caaaacaagc aatcgtcagc agctcggtca ggttcatatt gatacctgga
    14461 accacaggaa ctcgaattcg cacttcgcag ttaggatact caattaaaaa gcgacgaata
    14521 ttcttcaaaa tttgttggtt atctaagccg gtccattgtt tgtggagctt gctggtaaac
    14581 atttttacat ccacaaacaa tgtatccaca tagggagcta ccttttggat ttcattataa
    14641 gcaccacaaa agctggtttc cagtacggta ttgattccaa tgtatttcga tttttgcaaa
    14701 atttctttag caaaatccgc ctgaaccagt gcctcgcctc cgctaatggt cactccgcca
    14761 tccgagtgaa agtaaaaaac agcatccttc tcaatctcat ccattacctc ttcagcggtc
    14821 atggtctggc caaatccttt ttcaatctgc ttgctttgtg attcgggaat cgaacaccag
    14881 gcacagcgta atgggcagcc ctttaaataa acaacggtcc gtaaaccagc accatcatgt
    14941 agagaaccct gttctattct taatactgtt ccttccatat agatttttga attacacact
    15001 agtttaaact aaaacacaca aggaaaaact taccatttaa tcataaaatt agctcatata
    15061 aatatactag gatgtaaaat agactggtca aaatctggtc aaaaaagagg actaatacag
    15121 gtagtcctct tttttgttat tttaatattc taaatcattt tttattggtt tattccctta
    15181 ttcgatgtag actcaaatca ctatatcact agatatcaaa cggcgtcata cggaatcaaa
    15241 aacatcaaat ttgacctgtt aaatggtcat tgaataacat tcaaaatcaa taaaacggag
    15301 tcagacacgg agtcaaaaac gctgagagta aaaaatatat acagccttgt aggtaaagta
    15361 ccacagggct gtatattttt tatttcagat taccttaaat gattccattt actttttatc
    15421 tcattttttc ctactagtta gtatcaaata ctctcctatt ttctcaataa actatttttg
    15481 gatacatggt atctgaaaca gctaaaacct tgtcattaca gcaaaaaatc acgttatcca
    15541 gactgtagac aaagtccatt ttatggatga tgtctacagt cttttctttt ataatagatt
    15601 tatgaaaacg aatgcaagga gtgatagcta tgatgtctaa aaacaatacg aatggtagaa
    15661 atcaatttgc gatgcttacg attgatgatc tagtttctca agatcatctc gttcgaaaga
    15721 ttgatgccgc gcttgatttt gaatttattt atccaatcgt cgaagccact tactccgatt
    15781 taggtcgccc aagtatcgat ccagtgattc tgatcaaatt ggtgtttatt cagtatttat
    15841 tcggtatccg ttccatgcga caaaccatta aggaagtcga taccaatgtc gcttaccgct
    15901 ggtttttagg ctactccttc gaggagaaaa tcccccattt ctcaactttt ggaaagaatt
    15961 atgttcgcca atttagagaa acgacggtgt ttgaggatat ttttgcctat attttagaac
    16021 aagcagttaa agcaggtttt gtaacagagg ataatttgta tctggattct acccacatca
    16081 aagctaacgc caataaacat aagtttacga aagaaatgac ccatgatgaa gcgaaagcct
    16141 atcaggatga attagaagat gagatcaatc gtcaacgaat agaggctgga aaacgccctt
    16201 ttactttgga cttagaaaag gaagtgaagc tgaaagaaag aaaaataagt aaagctgatc
    16261 cggaaagcgg ctactacgtc aaaggcgaac gagaaaaaca atttgcttat tctgcccata
    16321 cttcatgcga cgacaatgga tttattctta gcacaatcat cactcctgga aatattcatg
    16381 acagtcaagt cgcctttcag ctcgtcaaac aatcgaagag actttttcct gaaatcaatt
    16441 gtgttgtcgc tgatgctggg tataagacac ctaagtttgt tcactttttg actcatttga
    16501 accttcgacc ttgtttgcct tattcaaggc ccaaagggaa aaaggggcta ttatcaaaaa
    16561 atgagtttct ctatgacgag tattttgatt gttatatttg tccgcaagat caaatgctcg
    16621 ctttttcaac ggttactcga gaaaggtacc gagaatacaa atccaatcca aaagaatgtg
    16681 tgaactgtcc tctgctaaac caatgtacca aaagtaaaaa tcatcaacga gtcatcacga
    16741 gacatgtttg gggagatctg atggatgaag tggaacatct tcgcctaaca gatctgaata
    16801 aatcaattta caaaaaacgg aaacaaacga ttgaacgaat atttgcggat gcaaaagaaa
    16861 agcatgggat gcgttggacc aagtatcgag gcttggaaaa agtcgcgacg catacgatgc
    16921 tagtgtttgc tgcaatgaat ttaaagaaat tagccacctg gctatggaaa ggcaaggagc
    16981 ctttattttt ttgttcaaaa attcgaaacg aagtagataa aaagctgttc caagctcgag
    17041 ttacgagttt ggaacagctt ttgtctacag tctgcgatgt cggagacatc tcgttttcta
    17101 ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc aatgtttact
    17161 ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt gtcatgtttc
    17221 tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc ttgaagcatt
    17281 ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc tacagattcg
    17341 ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt tcctttcttt
    17401 ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc gtgaagcaac
    17461 cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat ttttggtgct
    17521 aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt ccttcttaat
    17581 atattgaatg aagaaataat caatgataca agaagtacta aactcataaa tataactaca
    17641 taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt gttaaatcct
    17701 tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat ttgtttagct
    17761 atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt atattgagct
    17821 aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat taggaattga
    17881 ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc tttctttcta
    17941 gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat taatgagcca
    18001 aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa tgcagggtaa
    18061 tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt cccaagatct
    18121 tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc ttggtataca
    18181 gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt gattaatgta
    18241 ttttcgtata gttcttgtgc tgcacttact acaccgctat tatgcccatc gaatagacct
    18301 ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc aaataaatta
    18361 gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa atctttatgc
    18421 attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa tttatatgct
    18481 tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat aactgttctt
    18541 ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt ttcaataata
    18601 tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc tatactatct
    18661 gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct aggtgttcct
    18721 ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc aaatgtttct
    18781 tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa agttgacata
    18841 gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac ataagcaaat
    18901 gcatttttta tcattattca acctccaaat tcattttatt tacttttttc aactttttac
    18961 cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt gttacaacta
    19021 ttacacattt atttctatct ttagctaatg tctttaatat attaattatt tctccagcag
    19081 taacactgtc tagattacca gtaggctcat cagctagtat tattggagca tctgatacca
    19141 atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata acatttcttt
    19201 ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct gatttattta
    19261 ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt tgaaatacta
    19321 aagatatatt attttttcta tgattactat atcctttatt ttgtatatct tcattcttaa
    19381 acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga agtgtagatt
    19441 ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat ttttgattta
    19501 ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc tttatttcta
    19561 atatatccat tattttctcc taacttattt ttgataagat ttctttaggc ttcttgatta
    19621 acattaatga agaagcaaat acaactgata aaacaataat acttattaat attaaataac
    19681 tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa cttccaccgg
    19741 aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt aatacatttc
    19801 ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg aatataaatt
    19861 gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt tcataaattc
    19921 tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct aacataatag
    19981 aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa gactcttcaa
    20041 acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc tctttcaatt
    20101 tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt ttatttgcaa
    20161 tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct acaaacacca
    20221 tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta ccagaaaaga
    20281 tccctataat tttaaattta tgacttttta tttttccact tttttcaata tctagtaatt
    20341 caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct tcatgaacaa
    20401 taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta aatactccac
    20461 tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca acattcttaa
    20521 attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact ttagcatctt
    20581 ttaattttgc taatccatca tattgaatta ttttttcttc aatttctttt aatttttcaa
    20641 tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt gatattgaag
    20701 aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt attgttaaac
    20761 aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa gttctatttt
    20821 tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact cctctctatt
    20881 ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt ccacatctga
    20941 tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa acttttttac
    21001 attttccaca attaatacac ttatccttat cctgaatcgg ctacactaga ctagacagaa
    21061 aaaataaggt gtgtagaata ataaaaaaca cactggagga tttttcatgc caagacgtca
    21121 acgaagaacc tattcaaaag aattcaaaca acaaatcgtc gatctctatc tcgctggtaa
    21181 gcctcgcgca g
//
LOCUS       DACIFS010000045        20572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.045, whole genome shotgun sequence.
ACCESSION   DACIFS010000045 DACIFS010000000
VERSION     DACIFS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.045"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IYA83_001794"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IYA83_001794"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ8587223.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IYA83_001795"
     CDS             1085..2005
                     /locus_tag="IYA83_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ8587224.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IYA83_001796"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IYA83_001796"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ8587225.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IYA83_001797"
     CDS             3311..4789
                     /locus_tag="IYA83_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ8587226.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IYA83_001798"
     CDS             5066..7126
                     /locus_tag="IYA83_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587227.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IYA83_001799"
     CDS             7209..7778
                     /locus_tag="IYA83_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587228.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IYA83_001800"
     CDS             complement(7953..8759)
                     /locus_tag="IYA83_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587229.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IYA83_001801"
     CDS             8897..10411
                     /locus_tag="IYA83_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ8587230.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(10814..11797)
                     /locus_tag="IYA83_001802"
     CDS             complement(10814..11797)
                     /locus_tag="IYA83_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ8587231.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(11802..12449)
                     /locus_tag="IYA83_001803"
     CDS             complement(11802..12449)
                     /locus_tag="IYA83_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8587232.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(12534..13166)
                     /locus_tag="IYA83_001804"
     CDS             complement(12534..13166)
                     /locus_tag="IYA83_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ8587233.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="IYA83_001805"
     CDS             complement(13182..13733)
                     /gene="coaC"
                     /locus_tag="IYA83_001805"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ8587234.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(13730..14467)
                     /locus_tag="IYA83_001806"
     CDS             complement(13730..14467)
                     /locus_tag="IYA83_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ8587235.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(14464..15453)
                     /locus_tag="IYA83_001807"
     CDS             complement(14464..15453)
                     /locus_tag="IYA83_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ8587236.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15711..16253
                     /locus_tag="IYA83_001808"
     CDS             15711..16253
                     /locus_tag="IYA83_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8587237.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16270..17355
                     /locus_tag="IYA83_001809"
     CDS             16270..17355
                     /locus_tag="IYA83_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587238.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17367..18173
                     /locus_tag="IYA83_001810"
     CDS             17367..18173
                     /locus_tag="IYA83_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587239.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18174..19001
                     /locus_tag="IYA83_001811"
     CDS             18174..19001
                     /locus_tag="IYA83_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587240.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            18998..20071
                     /locus_tag="IYA83_001812"
     CDS             18998..20071
                     /locus_tag="IYA83_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8587241.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         6791 a         3597 c         4113 g         6071 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    10561 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
    10621 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    10681 ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca
    10741 attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt
    10801 gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt ttgtttgctt
    10861 ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca atgattggcc
    10921 gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt gcgagctctg
    10981 ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca cgtttcccaa
    11041 tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca atcaagaccc
    11101 aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg ccgatgaaat
    11161 ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct tgaaagtctt
    11221 gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg agtttttctt
    11281 ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat tggatcgctg
    11341 ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct tgtaattttc
    11401 caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca gcagcagaaa
    11461 catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca gcatattcgg
    11521 cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa gtggagatat
    11581 cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg tagggaggtt
    11641 tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct gtatggactt
    11701 tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt tctaatacat
    11761 ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc ctttgcccat
    11821 tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt cgtacgagaa
    11881 atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg agtgatcgtt
    11941 tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc atcgttagtc
    12001 aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca ttcatctgca
    12061 taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg ttcatctgca
    12121 taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc gatcactgtt
    12181 ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc ggcagcaaaa
    12241 atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca agttaaatca
    12301 aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac atctctcacc
    12361 cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg gccaatttgt
    12421 ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc aaatctttcc
    12481 attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga ctatcagtta
    12541 gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc agtcgcaaga
    12601 cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat agctgaataa
    12661 acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa aaagtagatc
    12721 atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa gaataaagaa
    12781 acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa ataaggaacc
    12841 accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct tccatgttct
    12901 gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg gataactgtg
    12961 ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag tcttggtcct
    13021 aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat tggaccgata
    13081 gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa catcgcagtt
    13141 aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt tctttcatta
    13201 agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc ttttccaaaa
    13261 tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac agatctcaat
    13321 gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat ggcaggggca
    13381 atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag atcgtctgca
    13441 atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag aaacaagtca
    13501 gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg catgacgtct
    13561 gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa atgtgtgctg
    13621 cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact tgtaagatca
    13681 gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt cattttgatt
    13741 cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt tgagcttctt
    13801 ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg atttccgtca
    13861 aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt gcgactgcca
    13921 gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt gcttttggct
    13981 gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc acaagatgct
    14041 cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc gcaaaccaat
    14101 tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt tcttctgaaa
    14161 aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca tcgtagtgtt
    14221 cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg atcgtataaa
    14281 tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg taatcgattg
    14341 tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt cctgtcgaat
    14401 gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct gtgattagaa
    14461 tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc cagcaagtag
    14521 tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca gttcatctgc
    14581 aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg gtacattgac
    14641 agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag tcgcgtttag
    14701 tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga tcaatggaag
    14761 aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt gctgttttcc
    14821 taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca aagcacctag
    14881 actttttagc catgtgacag actcttcttt tctggaaaag gcaatgattg actggaatcc
    14941 tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac cagtaacgac
    15001 aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca acatagcagt
    15061 aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg gtagaggaac
    15121 taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg tgacgccaag
    15181 cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt tactttggac
    15241 gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca cctgaccaga
    15301 ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt ttaccaagac
    15361 ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga aaacaggttc
    15421 ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt taaaattttc
    15481 ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt tagacaaaaa
    15541 ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa agaaatattc
    15601 agcaaaaaaa attttacaaa gggttgcctt tttcaataaa tttcgtatac taatacgagt
    15661 ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt atggaaattg
    15721 gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa ctaggcgaac
    15781 gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt tcaccatcaa
    15841 tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa ttttttcatc
    15901 aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt tatttcgatg
    15961 aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa gaaatggagc
    16021 ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct tcattatctg
    16081 aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag acggtctatg
    16141 tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa ttaaggaatc
    16201 atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg taatataggg
    16261 ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg ctatgatgat
    16321 gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta tacactgctt
    16381 ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt caccgaagct
    16441 acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc taataagcga
    16501 caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt ttttgataac
    16561 gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa gaaggtaaaa
    16621 gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag tgaaatgtct
    16681 ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc gaaagtattg
    16741 ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat gcagtatgaa
    16801 ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca tgaccaagaa
    16861 gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat cgtccaaagt
    16921 ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga ttttgttggg
    16981 gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt tgttggtaag
    17041 caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt tgttttacgc
    17101 ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac ggtcgatact
    17161 cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca aaatgaatgg
    17221 atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc ctttgagcca
    17281 gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc acgtcttgac
    17341 agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat tccgtccctt
    17401 atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt tatcaatctt
    17461 tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt acgtcgggga
    17521 catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg tttttcacgc
    17581 tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag caattatggc
    17641 tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac gcatttattg
    17701 ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg atcgggccga
    17761 aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc gagattccat
    17821 ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg atcagtgcta
    17881 gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt ccgttatcat
    17941 taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct ttattcatgc
    18001 tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc gaacagcatt
    18061 tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta atcattgcta
    18121 tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca taaatgaaaa
    18181 aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat gcaccgatct
    18241 tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt acaggattta
    18301 cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc gtattgaata
    18361 cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca tttggggcta
    18421 tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc atgaataaca
    18481 tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc tttacactac
    18541 taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc agcattccaa
    18601 tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg gtcgatgcgg
    18661 ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg ccttatcttt
    18721 ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat gattttgcgg
    18781 ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc tattctcgtg
    18841 caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc ttgttctcca
    18901 tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa cggctgaaga
    18961 aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac aatctctgtt
    19021 tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac ttgaaaaaac
    19081 aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt atatcgatcc
    19141 tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg aaacatttga
    19201 ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg acatcgctat
    19261 accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc cattagatca
    19321 ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg attttgatcc
    19381 agataatcag tattcgattc catatttttg gggaacttta gggatcgtat ataacgataa
    19441 attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg aattaaaaga
    19501 cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga atagtttagg
    19561 ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata aattgaatca
    19621 tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata tgatcaatga
    19681 agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt gggaaaatga
    19741 acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata atatcgttat
    19801 gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta tgctagaacc
    19861 ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg aagaagcaaa
    19921 agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg atgacacgat
    19981 ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct ataatgacct
    20041 attcttagaa ttcaagatgt ataggagata atggcaaaac tcagtaagaa tggggactta
    20101 tcaacgttct aaagagctaa aaatcgtcct ttttctatct gagttttcca atcttctacc
    20161 aaaagcggat gaaacgccgc tttttttgtg ttattttatt ttttctacca cgtttttcta
    20221 ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg agacgggcta gtaaatctaa
    20281 gattgaaaaa gttacagaag gataaagtaa ttggaaacta tgcaaatata cttaaccctt
    20341 ctgtaatcta ttaataaaac taatatatga atccagttca atatttgtag ctatgaaaaa
    20401 acataaaaat catattagat aaaaactaaa ttggtagata aattattttg tcagatatct
    20461 gaggcaaata ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa tatagaatag
    20521 acatattgca gccgatagtt tattatgctt ttagacacat taaactggag ga
//
LOCUS       DACIFS010000046        20458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.046, whole genome shotgun sequence.
ACCESSION   DACIFS010000046 DACIFS010000000
VERSION     DACIFS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.046"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(164..778)
                     /locus_tag="IYA83_001813"
     CDS             complement(164..778)
                     /locus_tag="IYA83_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587242.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            922..1484
                     /locus_tag="IYA83_001814"
                     /pseudo
     CDS             922..1484
                     /locus_tag="IYA83_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="IYA83_001815"
     CDS             complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="IYA83_001815"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ8587243.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2588..3043)
                     /locus_tag="IYA83_001816"
     CDS             complement(2588..3043)
                     /locus_tag="IYA83_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ8587244.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(3071..4516)
                     /locus_tag="IYA83_001817"
     CDS             complement(3071..4516)
                     /locus_tag="IYA83_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8587245.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="IYA83_001818"
     CDS             complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="IYA83_001818"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ8587246.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5616..6374
                     /locus_tag="IYA83_001819"
     CDS             5616..6374
                     /locus_tag="IYA83_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ8587247.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            complement(6869..8068)
                     /locus_tag="IYA83_001820"
     CDS             complement(6869..8068)
                     /locus_tag="IYA83_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8587248.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8511..9002)
                     /locus_tag="IYA83_001821"
     CDS             complement(8511..9002)
                     /locus_tag="IYA83_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587249.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="IYA83_001822"
     CDS             complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="IYA83_001822"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ8587250.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            complement(10189..11556)
                     /locus_tag="IYA83_001823"
     CDS             complement(10189..11556)
                     /locus_tag="IYA83_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ8587251.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            complement(11581..11859)
                     /locus_tag="IYA83_001824"
     CDS             complement(11581..11859)
                     /locus_tag="IYA83_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587252.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            complement(11878..12336)
                     /locus_tag="IYA83_001825"
     CDS             complement(11878..12336)
                     /locus_tag="IYA83_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8587253.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            complement(12348..12968)
                     /locus_tag="IYA83_001826"
     CDS             complement(12348..12968)
                     /locus_tag="IYA83_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ8587254.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            complement(12979..15021)
                     /locus_tag="IYA83_001827"
     CDS             complement(12979..15021)
                     /locus_tag="IYA83_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ8587255.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGKDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            complement(15399..16538)
                     /locus_tag="IYA83_001828"
     CDS             complement(15399..16538)
                     /locus_tag="IYA83_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ8587256.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTLAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            complement(16535..17119)
                     /locus_tag="IYA83_001829"
     CDS             complement(16535..17119)
                     /locus_tag="IYA83_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ8587257.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            complement(17309..20350)
                     /locus_tag="IYA83_001830"
     CDS             complement(17309..20350)
                     /locus_tag="IYA83_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ8587258.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
BASE COUNT         6208 a         4345 c         3393 g         6512 t
ORIGIN      
        1 tacaaaaata tttttattgt tggatacaga aatctatttt taactgggga tatctttgcc
       61 agctaatatc tttgagacta cacctatctt catctaaatg gatcaattac tcgctttttc
      121 cattgacctt cgtggtactt ctgctttctg atagtaaaaa acattactga atgatcttac
      181 tatataacat acagcggaga tctacttctt tattaaggat cttcatgacg ttatcttttt
      241 cttttttccg ttcgaaaccg cgtcgttcat aaaattgata cgtacagccg ctatccgtga
      301 acagaaatac ttcttttcct tgctctcggc gttcaaattc ttttaataat ttactaccga
      361 tccccttgat ctttaaatca gggttggcag ctaaaaagac aatttctcca tctggggaat
      421 gtttttttaa atagtctgaa agcaattcgt cattggtgtc gtcatacccg cctttgctgt
      481 ctttcacaaa tgcattttgt aagaaatcaa atagtttaac atataacttt tgactaaatg
      541 aatgatgctt ttttgatttt cctttgatgt ctgctaataa aacacctgcc aactcatcat
      601 ccgcatataa agcaatgatt tgtgtcgcgc gattcaattc tagataccaa aagtatcttc
      661 cataaagatt caaaaggagt ttgttcttgg tataccaatc aaagtgcata cctttaatcg
      721 cgaattgaat ggcttttttg tagtctttct tttccagttg cttaaaggct aattccatca
      781 cttattctcc tattctttaa atattgatat gctatactta tattataata gacaaggtgt
      841 cggttatcta tgttcagata gcggtgaaat tgtcggataa ccgacacaat tccttgatta
      901 tgttgattag gaggctgcaa aatgaaaaaa gaggatcgcc gcgtcaaaaa aacagaaaaa
      961 gcgttagcag aagcattatc aaaattatta gtagataaaa aaattcaaaa catcaccatc
     1021 cgtgaactga ccgaaactgc tgatcttcac cgttcgacat tttatacaca ttatacggat
     1081 atctatgatc tttatgaaaa actggaaaat agctttttcc aagacttgga tgaaatcatt
     1141 tcctacgata cgacacatag ctacgaggat ctatacgctc aattgatcga ctatgtctat
     1201 acgaatacgt tcgtatacaa gttattcgtc atcaaccacg gagatatcca atttcaaaag
     1261 agattaaata atctgatcga agaaaattat ttaaaaatct ggctacaaga agacaaaaaa
     1321 aaccgtgatc acagaagaaa tgcgttactt caccatctac catatccaag ggtgcctctc
     1381 cgttttaaat cattgggtag aaaagcaatt ttgcatactc taagaaaaaa atgctgacct
     1441 tcttgcgctt attgaacgac aacatagaac ctatcatgcc ttaaaaacaa acgaaaaagg
     1501 aagcgtcggg acgcttccaa ttctttattt aaaagctcaa attgattatt aaaaatcatt
     1561 aggttacaaa cttaaggtta tagttaaagc tttcttacaa tttaatagct gttgctgttt
     1621 ctttcaaaac attattcagc tcttcgatgt tcttgcgtcc ttgtgtctgc atccattctt
     1681 tggcagcata ttcaccttta gtaacgaatg gttctacacc attcgcccaa gttgcgcgcc
     1741 cacagagtac cccattgaat gtagagccag cctcttttgc aaatttcaag gtttcttgga
     1801 acaattcagc agttactcct gcgctcaaga aaataaatgg caggttggta gtctcggatt
     1861 gttctttgaa ataagcagca gcttcttctt tggtataagc aacttcattt ttagtgtaac
     1921 catccacata gttcatatta acaggaactt ccactttcaa aacgtcaact ccatagcgag
     1981 gcttagaaaa ttccttcatc atttcattta ctttatgcgg ttttttcaag gcatattcct
     2041 tcgaagaagt atcaggatcg ttggcatcat aagaaaccaa ttctaagaaa aatggcaaat
     2101 cttcagcaac acattcagaa ccaatccgct ccataaacgc cttcttttgg tcattgatgt
     2161 catcagattc atctacatcg tagtataaca agaacttaca agcatcggcc ccttgctcct
     2221 ttagacgttt tacagaccaa atagataaaa tatccggtaa acgtcctgga gttgaagcat
     2281 cgtaaccagt tttttcatag gccaacaaca gaccagcatt tttatcgcga acttcggctg
     2341 ccggcaaccc atattctgga tctaataaga ttgctgaagc atatggagtc aattcagaag
     2401 aaatgatttt tttgaaattg atgatttgtt catcggttgc tgatttattt tgaaatttac
     2461 cgatcatttt tttcaaggca ccacgttgat cgattgctaa cgcactgatg atgccacttt
     2521 cattacttag ttgacgaata taagttttct ttttttctgt taaaggcatg atttcactac
     2581 ttccttttta gttttcttct aatttttggt ttaaaagttc agctatttct tcagcagact
     2641 gggcttgttt cagaccttct gtcacatctt tgtgcatcaa aagtctggct acctgagata
     2701 aaaattttag atgggttgtt ccagcctctg aatctggaat aaacagagcg attacaaaat
     2761 ccgttccttc tccatctaaa cttttccaat cgaccttacc gtttaaccga acaatcacaa
     2821 tatcggcatc cttgatacta gcatctttgg catgagggat ggcaaaacca tccatcatac
     2881 cagtcgtgct ttcttcttct ctttccatca atttttcgta aactgcatct cggtcttcac
     2941 taatgctcaa gacaattgac tgatctgcaa ggaagttcat tacctcatct tgcgtcgtaa
     3001 aatttttatt cataaaaact ttatttgttt tgatcaaagt catttgaaat ccttctttct
     3061 agaataacct ttagttgttg gttgcttctt tcatttcaac agcagctgct tctgcttcac
     3121 gtttttctac actacgtttt tggatcaacc ctaaaattac tccgccaacg attgaaccaa
     3181 gtaaaatcga ccctacccat gcaaccttac cagtaacaac aggtaatact agaaaacctc
     3241 catgaggtgc gggaacttgg acaccaaaca tatatgtcag tacagcagca attgatgaac
     3301 ctagcatcat agttggcaga acacgcagcg gatctttagc cgcaaatgga attgctcctt
     3361 cggtaatgtg cgttgagccc aagataaagt tgactaaacc agcgttgcga tcattcgtgt
     3421 cgaaatattt tccaaaaaac agtgcggcaa atccagtaat caatgggggc gcgatacaag
     3481 ctgccgaaac ccctgccata aatgtatggt taccttgtgc caacaacgca gtccctgtaa
     3541 cataagccgc tttattgact ggacctccca tatcaaatgc tgacatacag ccaacgataa
     3601 tccctaaaat taatggatta gaattttgga atcctgccaa gaaattcatc attccttggt
     3661 tgactgcttc cattggattt gctaataaag acatgactac accaacaaac aagacaccga
     3721 tcaccggata taagaagatc gccttcagac catccaattg tttaggcaag cctttcatga
     3781 ccttttgcaa tagtaagata aagtagcctg ctaagaaacc tgcaaaaatt ccgcctaaga
     3841 aaccggctcc gccagaattg gctaacagtc cgccaacaaa cccaacaacc aaaccaggac
     3901 gtttagcgat tccttcagcg atataagctg ctaaaatagg aaccatcatt cccattgccg
     3961 catcaccaac accctttaac atagcggcag tcggattgta agtaggatca ttgggatcaa
     4021 aagagtacac accccagatg gcaaacgaaa ttgcaataat taccccacct gccactacaa
     4081 acggcagcat atgagaaaca ccgttcatga gatttttata aatactgtga ccaatgcctg
     4141 ctgcaacccc catctccgct ttctcaccag cagcgtcaac tgttttgttt tctgcagtga
     4201 ttgaaccttt accagccaac gcatcttcga tcaattggtc agcatcttta atccccttgc
     4261 tgacaggcac atcgattaca cgcttacctg cgaaacgttc tgcttgaaca tctttgtcag
     4321 ctgcaatgat tacagcgtca gcgtttttta tttcttctgg tgttaattca ttctctatcc
     4381 ctacttgacc atgggtttca actttgatag ataccccttt gcgcttagct gcctgctcca
     4441 atgcttcttg agccatataa gtgtgggcaa ttcctgttgg acatcctgtt gcggcaacaa
     4501 ttttgtaagt tgtcattttt tactcgctcc ctctttgatt tcgatttgtt tttccaactc
     4561 tgcaacatcc gaaaaatctg ttaatccttt acgaaaagcg gttgaacttc cagctgctaa
     4621 acttttcttc aaattaatat ctacctgttc accttttaaa tatcctgcaa gaaatgttcc
     4681 caacaaagtg tcaccagcac acgctgtatt gactacttta cctttaggag aattcccaga
     4741 ataaacgata tccttattta aaaatattcc tccctctcct cctagagaaa ggagaacatt
     4801 tttcgctcca tcggcaatca agcgttcagc ataatgaata tatccttcac gatcactaag
     4861 ctcgacatta taccaagatt gcaattcctc ctcgtttggt tttaataaga acggctgata
     4921 cggcagacaa tcaattattt ccggatagct tgaatcaata atcagatgga ccttattttc
     4981 aaaacaaata cgactgatat caataagaat ttcgggtgag acccctcgcg gcaaactgcc
     5041 agaaacacac aaataatcgt cagcttgcag cttttgtatt tgatttagaa aagttttgat
     5101 ttgttcctga ggaatctcag gtcctttatt aaccaatttg aattcttcat tggttttatt
     5161 aacttgtgta aaaacgttga ttctagtcat tcccggtacg ctaataaaat cagtgctaat
     5221 atctttttga tgcaaataat cctcaatata attcccggta aaacctgctt taaatcctaa
     5281 agctgtacta tcgataccta attgcttcat gatcaatgaa acattcacac cttttccatt
     5341 agcttgtata tcatcatcgt ttgttcgatt gacttgggat ggtttcattt catctgtttc
     5401 gataaaaagg tcgattgcca agtttaaagt acatgtataa actgacattt acgctcctcc
     5461 tttcttacat attcattatg tatcaagatc gaatgtaaat gttttcataa atgactaaat
     5521 tcattgaaaa cttttacaaa atatattaaa taaacttgaa gtaaaggaaa ctcccgcttt
     5581 acaatagaat tatcctaact aatcggaggc aaaatatgga tacaaaatta agtgaatcgg
     5641 agaaatatct ctgggatttt attcaaagtc acatactcga aatcccctcc tattccattg
     5701 taaaactaag tgaatatgcc aacgtttcaa cagcaaccgt cgttcgtact atgaagaaaa
     5761 aaggatatga ggggtttacg tcttttaaac attcgttaaa agaaaaagaa aatacaaata
     5821 tcaattttgc cgctcttgat aaagttgatg aaggaataaa gactgctatt cttaaaaatg
     5881 agcaagaggt aatccgcacc atcaatatgg tagaaagcgg gaatattgaa gatgctattc
     5941 aacgaatcaa ggctgctaga agaatcatga tttttgcccg aggtttttca gaattgattg
     6001 ctcaggaaat gatggtcaaa tttcaactta ccggtaaata ttgtgagatg catactgatc
     6061 ccaatatcat aaaaaacatt agtagaaaat tatccaaaga tgacatcgtg atctttgttt
     6121 cactaaacgg tgaaacaaag gaattgacta ccgcagctga aaactgttat aaagctgaga
     6181 tcgggacaat tttgcttact gctaatcgtc attcaagttt gatggaacat atcgagattc
     6241 cttttgttgg ctttaaatca gaaggctcct tcttcccaga ttatgaggta cgctccagac
     6301 ttccactttc tatactggct agaatcttac ttgactccta tgcactgcga acatcaggat
     6361 tcacacagca ctaactctat atctgtaaga ctgtacctaa aatatatctg tgtgaattaa
     6421 ctaaggttgt ctagaaaaat atttaatcgc tatttgtatt ctcgtgcttt ctatcattgc
     6481 tccttctata acttcttagt ttcaagatag aatagtgcat tgagactacc aggactactt
     6541 acgcaaatat ataataataa gaatgtgata agcagttcga agttcctgtc taatatttct
     6601 tgttaattat tatcaatttc caacaattct agaattataa tacgcaaaaa aaggagaccg
     6661 tctaaccgtc tccttatagt ttattattca gattacaatc agttatctaa aatgttacag
     6721 aagttttatt agggatattt tcttatcttt ttagatgagg tttctcaatt atttagaaat
     6781 gcttacttaa gttattctcg ttttttgtaa ttacgccaat aatcactata tgttatttat
     6841 ataaaactag tatctaagta tttttatgct actcaacaag agaatcacta tcgtttcctg
     6901 tcttcggtat ttttcgaatg cccaaaatag ttaataaagc acctagcacc gcaaaaccag
     6961 taccgaaaat cagcatctcc catacaccga aatgctgcag cagactgccg cccaagacat
     7021 ttcctatcac cccaccaaga gttgtggcga tgatgatcat cgtttgtcca aatacacgat
     7081 catcatccgc cagaaattga ttgaacaaat aagccgaagc tggaataaac aaagcgaaac
     7141 tgaaggcttg taggagatgc gtcatttcca tcatcaggat cgaactggcc agcagcatca
     7201 tgaatcctct gacagcaaat gaaatactgg caacagacag ccaaaatttg ctggatttct
     7261 ttttgacaag ctgttcaaaa aagatcattc ccggtaattc gcataaagcg gcgatcatca
     7321 atgcgatgcc aacttcttta ctgccgccgt ggatattttc aaagatctgt gccaaaaagc
     7381 tgttgatcat ggtgtggaaa gtaaaaagaa aactgaaacc taaaaataac aaaatcagga
     7441 aaggatattt ttttctcaaa gaaactgagc gatcctgttc ttttgtcata ggaaaaactt
     7501 cttgattttt tacatccggc agcagatgca aaataacgaa aaatatcact gcttcaacta
     7561 taaccaagat tggcaaaatc gcggcagaat aaatcgctaa cagctgtccc agtaaaaaag
     7621 aagttacagc ataagaaaat gaaccaccag ctcttgaaaa gccaaagttg atcctgtagc
     7681 cggtatttga atactcaaat gccagcgcat tgagaaatga ttgcatagaa actactaaaa
     7741 tcccacccaa gatataagta atgatcggca aattcggaat caatacccca gctaagagaa
     7801 tcactcctaa tcctgctatc atcgtcttca gcacggtgat cagtgggatc tttcttttct
     7861 tgatgatcac ttgactgaca aaaggctgaa gcaccgttga aacaatattg accaatgcta
     7921 agatccctcc gattttagct ggatcaatct tgaaataaat caaaaacaat gtcgcaaacc
     7981 catagacaga acaaaacatc atccaataaa gaaaatgcaa acttgtataa taaaaacgaa
     8041 tcatttttcc gcttgtttga tcgcgcacac atctcccacc ttattggttt ttctttagca
     8101 atttactata gcatacttgt aggggtttta ctatttttaa aagtggtgaa gaactatgga
     8161 aattagggta gattacaata tgcgttgatc aattaaaaca tctgttaata aagacggaaa
     8221 aaagatcata gaagcatcgt acttttttag catactagct tggtcatttt tttctggaat
     8281 tgaagaaaat atatatattc taaaaactat ccatcatctc ttagattacg agcttttttt
     8341 gctgcaaagg aagttaaaaa aagcagggat cgagtaatcg attcctgcta tatcttgtac
     8401 caatatattt ctttccaaaa cttaatatac attgtatact tcaatcaata tgattcatta
     8461 aaacaagttt cattattcgt gataaagaaa tcataatttc ggtagtagaa tcattcatta
     8521 tcttttttta caactgctct tttttcttcc gaactttgat ctaaagtaat tttggaatgg
     8581 atatcaagtt cttgaaagac atccatcatt tcagtttgat ctttatcaaa aatcattgaa
     8641 aaagaattat tcttgctttt gtaaaatgaa ataaatgatt tatcgtcacc tagtttttct
     8701 acttggactt gaggatatcg attaaagtta ccgttcaata ctccaaatct aacaacagaa
     8761 tcttttgcta gaccctcctt caaaaaacca ttgatcaaaa tgacagaagc aaaagcaacc
     8821 agtttaatct gatcagtcag atgttccggc cagaaaatca acaggatggc gactgtcagt
     8881 aaaatactca aactcttcct tacaggacta ctctgagcaa acacgataat tttcttttga
     8941 tcgattattt gcaaaataat cagcagtcct aaaaaaactg tgatcataag tgcttctgcc
     9001 atctttcatc atcctttatt ttcatttatc accactcagc gatcgtaccg tcataatttt
     9061 tccattgcgg attggtccaa atcaaacctt cttgtgcaat ttctttgatt ttgtcttcat
     9121 ccatttccaa gcccagacct ggttttgaag gtagatctac gtagccgtct ttatattgga
     9181 aaacttcttt gttcttcaca aaatctaaca gatcaaaacc tttgttatag tggatcccta
     9241 agctttgttc ttggataaag acgtttggcg tacatgagtc tacttgcaga gttgctgcta
     9301 gtgcaacagg accatatggc gcatgaggcg caacagccat gtcgtaagct tccgccatcg
     9361 ctgcgatttt acgggtttcc aagattcccc cacaaagagc cacatctggt tggataatat
     9421 caacagtacc ttgtttaaag aggttcttga attgccaacg agtgtacaag cgttcaccag
     9481 tagctaatgg tacagccaca gaatcagcta cttctttaaa gtgttcttca ttttcaggta
     9541 aaacgacttc ttccaaaaac attggatgga atggctccaa agcttttgct agcacttttg
     9601 ccatcggttt atggacccgt ccatgaaaat caacaccaat atccattgca tcaccaaaac
     9661 gttcgcgaat agacgcaact cgttccacca cttcatcaac tttttggtag gaatcgatat
     9721 aatgcagctc ttctgtcgcg ttcatcttga cggccgtaaa gccccgatca aaccgatctt
     9781 gtgcttgttc tgcaacttca cttggtcgat ctccaccaat ccaagaataa accttgattt
     9841 tatcacgtgc tgcaccgccc aataattcat aaaccggaac attaaacgct tttcctttga
     9901 tatcccacag agccatttct aaacccgaaa cgatggttcc attaatagga ccgcctcgga
     9961 agaaagatcg atgcatttct tggaagaggc gctcgatttc aaacggatta cgtccaatca
    10021 gccggtttcc tatctcataa gctccagcaa caaccgtttc tgttttagtt cctgagatca
    10081 tttctcccca gccttcgatt ccttcgtcag tcgagatctt aacaaaaatc catcttggtt
    10141 ttactttata cactgtgaca tctgaaattt tcaatttaaa ccctcctact attgtgcctt
    10201 caatggttca tccatttcat gattaacttt tttgatacca tcgaagtaat accatgcgcc
    10261 cgcaaaggct aataacaaca ctggaatacc gatgattggt ttgtaagcaa atgcgataaa
    10321 taccaaataa ctttggatca tggaagtcgc agcaaatgat gagattaaca ttgaagaagt
    10381 tcctaagtta ataccaaccg atttggcaat tgctgtcagc attggcgcag tagctgaccc
    10441 acaccatagc aatgaagctg tcacaactaa gccaataatg atatttttta ataaattacc
    10501 acgagtcaag gcaacgacca ttgccacgcg gaaaggaaca accgctaaat ctgcgaatgg
    10561 taacacacgg tttcctggca atactaaagc taatgcaatc gttagtggaa taatgatcaa
    10621 tgctgttgtg ataacatctg gattacctac aacaactgca gcatccaatc cgataaataa
    10681 acgtgtacct ttgaattttt tttgtgacca tttttgtgca gcatctgaaa ttggcatcaa
    10741 tccttccata aataatgaag tcatcttagg aatcaaaacc aaaacagctg acatgctgac
    10801 acctaattgt aaaaccgctt tgatatcata gccggctaaa acaccaatca ccatcccaat
    10861 aactaacccc atgatcatag aatcaccaaa aaatcccaaa tatttttgtg catctttgat
    10921 ggtgaatttt gattttctga ataacgggat acgatccagc agccaattta acggccaagc
    10981 aataaccagt gatgaaagcg cagaaacagt tggtaaagaa acaccaggga ttccgaagaa
    11041 actttctacc atcggttgcg accagtctga aatcttaaaa gtcgcaattg ccatcactac
    11101 tgatgcacct acacctaaga agacattctt agtcacaaaa tacactataa caccaacgat
    11161 tgccatatgg tgatagttcc agatatcaac atccagcgta tgtgtacgtt tcaaaatcag
    11221 caaaacgata ttgaccaata gaatggcaaa aatcaagatt ggaatgattg gcgatgccca
    11281 agtaacagcc gcaatcgaac cccagccaac atccaaagca tctagtttaa caccagttcg
    11341 atcaaccatc gccttggcag ctggtccaac attggtcgtc ataaagtcca atatcaactt
    11401 gatcccggca aaaccgatac ctaatgtcaa acctgattta aaagcacgag aaatctttaa
    11461 cccaaaaatc aaaccaataa tcgtgatgat gactggcaac ataactgttg gtccagcatc
    11521 aataaagaag ttaaagacgt tcataatgat tttcattttt tcagctcctt tgatcagcta
    11581 ttatttcagt aagttcaaaa tctcttccat tgtctgatcg gcaccgatac ctgttaaaat
    11641 cggcatccct ttgattactg gtacaccacc tgtttgacca tcaaattgac cagtcgtcac
    11701 caatagatca tagtcttgaa cttttcctgg aacttccatc aattttgttt gagtcgttga
    11761 aacatcaatg ccatgttgtt gcaaatactc tctaactttt gttgctacta ctgttgaagt
    11821 ggcgatacca tttccgcatg ctactaaaat tcgtttcatt atgatttcct ccttatatta
    11881 aaaaaccgct ttctttaata gttctaataa ttcagtatca ttgcctgctc tgatcatttc
    11941 ttgccgcagt ggctcatctt gtatgattcc aacaattttt tgtaacattt caacttgccc
    12001 atgtggttcc ccaatcgcca tcatgatcac aatttggatt ggtaactgct cattgttatc
    12061 ttccatatta tgaaatagga ccggatcttt aagagtcgca attgctaaag ttgttttatt
    12121 gaccatttca tagtttgcat ggggaattgc gattcctggt ggtgttgatg gtaatccagt
    12181 agggtattct ttttcccgtt cttggattgc ttgaatatat tcctccttca cgtagttttt
    12241 tttgtacatc tcgcttgcta aaaacgctaa gacttcacta tctgtttttg cttgaacatt
    12301 tcgaaaaacg agttcctcat tcaaataaag ttccaaagtc ctcactccta tttaatagcc
    12361 tcaattaatt ctttcatcga acgtgctaag tttttaacat ttagctcttc gatgtcttgc
    12421 ttgttgaaca taccggaacc aagacctaaa tagctagcac cttgttcaaa aaactctttc
    12481 gcattctcct tactgattcc accaaccccc attagtggta acttacctaa aggtgcttga
    12541 atatccttga aaaaacgtgg agtaacaacc gctgcaggaa atacttttac aatatcagct
    12601 ccttctttaa acatacggtc gacttctgaa ggagtcattg cggcaggtac cgccaataat
    12661 cctttcttat tggcgtaaga caacatttct tttgtgaaca tatgtggtcc taataaaaat
    12721 tttgcaccag cctcatagca ggcttgggct tgttctaatg tccttaccgt cccagcgccg
    12781 atataagtcg tgtcaccata acgttgattt agttcatgta tattttcaag agctcctttt
    12841 gtgttcattg tcatctctac ggcaaattga tggtcaaatt cctgcattgc ttgtaaaata
    12901 gcatctgcct gatcaaaagt ataaccacgc atgatcactg tcaatttagg ataatctgct
    12961 agtctcatgt tttcctcctt aagatctttg tcttaatagt tgataaactg tcttttcgtc
    13021 atcagcaatc aataactgct caatcaatga atcatctttg gcgatatcag cgatttgatt
    13081 gatggctcgc agatgcttcg ttaaatcaaa aaatgaaagc ggcaaaatca agctgacatc
    13141 tttaccatta ggaaatcgta tcggcttttt acaaactaaa atgctgacac cgtcactgat
    13201 tacaccttta tcgaccgttg tatggggaat gcaagtcttc attcccaagt aacctgcata
    13261 tcccggttgt ctgatttgat ccaagcaatc agcgataaaa cgcgagtcaa tgatttgatg
    13321 ttccaataac ggtcggcaag ccacctccac tgctcgttcc caagacatct cttcttcaac
    13381 gatttgtaca taactcggcc gcaagtaata agtcaatgaa ggcaatacat tgaagttgtc
    13441 cagcggcgtg cttccatctt ccttgatcaa aaagtactgc agttcttctc gcaacgaaac
    13501 tggattggtg atatttgcat atttcctaat gattgttaac aattcatcca ttgctcgatc
    13561 aaccttagta attcctaaat cactcaaaac tcgatatcgt aaactgattt gttgctgata
    13621 tgtcatgatt ggctcgatca taaactgcag catagaactt tttagtggcg tcgtagtaaa
    13681 gaccagatca taatcagctt caaatttgta gaaatcacgc acagataggg aagctaaaaa
    13741 atggatctct ggaaaaagtt tttccatatt ggctcgtact aactttgaga ccatgacacc
    13801 attagtacaa acaactacag ccctttgtcg ttgcttattc aatttattat gattacttaa
    13861 ttgatagcca aagtaatagg ataaaagatc taattcatca tttggaaagg ccttatttag
    13921 ccaattctca ataggaataa tcaattcttt catcaaatca atcaaaatcg catgattact
    13981 atccttgatc agactatcta ttgaataaac tcctaaactc aagttatagc gtattcgaaa
    14041 acaggctgga cgcagatgac tcagtatcct acgttcaaag tcttctcggt catcgatcaa
    14101 aatcaacgtt tgattttgga aattttcaac catttggctg atcaattttc gcaattcttg
    14161 atccgaatca taattttgag tagttttgct ctcaaaaata ttggaagtta aaaataataa
    14221 cgtgaaccac tccatataac ttttggatag actccaacct gtttcatgta ccaagatcat
    14281 cagcattcgg aattcaggag tatccgtcac atcatcggga aaaaacgcgt gcttatctga
    14341 catcttgatc ccacggctta ctaaaaatct cgctgatgtc tgcaaatagt ctaccgactc
    14401 atcagaataa ctaagatgca acatttgttc catgttatgg atcaaatgga cgatttcttc
    14461 ttcagaaaca tcttgtgcca attttctgcg aatcatttct cggcgaaaaa tcggaaaccg
    14521 ttttactaga tccgataaca gctgtaagat acggtgttcc gaaccaacca attggtaacc
    14581 attcatccga ttatatcgaa ttgtcagact ataatttctg atgaacggtt ccgttcgttt
    14641 gatatcttcc ataacagtcg tcttactaac atcaaccgca tctaccaaat gatccagact
    14701 gatgtaatcg atattagcga tcagagtcat cactatcaat gcttgtcttt cactatcagt
    14761 gagaacgaca agttcctgat catcacctat gtcgtaatca ggctgtgata tcatttggat
    14821 aacctcgatt ggtaccgtaa tatttccaga atgacttctc tgaattaacg gaatgttctt
    14881 tgttttcaat tcttcattaa attgattgat cgcgtaattg atttgacgac gtgtcaaatc
    14941 aagttcactt gttaaagtgg aaagtttcat ttcagggttt ttaataatca gtttgagtat
    15001 ttcacgaatt cgtttattca ttttgactat cctcctgaca aaatcattat atacttgttc
    15061 acatacgttt ccggaaagct ttcatcacgt tttttgtcac acttggttta atgttttgtc
    15121 acactaacag ctatttttaa aatgaacgct gttatatcaa atgtaacatg gtatgacaat
    15181 tattttaggg ctaatgtgaa tattaaatat tcggtagaat attggatgct ttagtaaata
    15241 aacacgggac gttgcttctt caacacatta aagtatctat tccgtaacta aatcaagtca
    15301 gtctaattga aaaagaataa tattaaactg ctaaaaacaa tgcttttggg agcgtcaata
    15361 attttgtgta aatggttctt ccctactgta gattgtttct aattctccat cggttgactc
    15421 agcatttgtt cgagctcgcc ttcggcctgt tggaaacctt tatgactgcg acctaggaat
    15481 ttttggttgt aagtatcaaa agacgagact aagaaacgtt ccatcgactc ttcattttgg
    15541 aactgttcct tgcggtggct gtatttcttg atttgcttat taaaggattc aatcaagttc
    15601 gtagagtaaa tagtcttgcg tatggctaat gggaaatcat agaaagtgag caggtaatcg
    15661 ttcgaaagga tcgattccac catttttgga taactggttt tccatttttc cgcaaaagca
    15721 ccacgtgctt ccaatgccgc ctttttagat gacgcttgat acaccatttt aaaatcatca
    15781 cagacttcct tacgatcatc gacacgcact ttgtgactga tattacggga aacgtgcaca
    15841 cagcaatgtt gaaaacgggc tttggggtag aaccgctgca cggcccctgc catccccttt
    15901 aagccatccg tgatgaaaag gaggacgttt ttcacgccac gttcctggag gtctattaaa
    15961 atctcttccc aaatcgtgat agattcagtt ggtgcaatcg catagctcaa cacttccttt
    16021 gaaccgtccg ggcgaatgcc aactacgata tgaatggctt ccttggcgag ggttttccgc
    16081 tttaaaggga tataggttgc gtccatataa atagcggcat aacggtcatg aagctcacgt
    16141 tccttaaatg aagtgacttc ttccgtaaat gatttagtca tattggacat ggtttggggc
    16201 gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat ggtaatacct
    16261 tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct ataagcagga
    16321 acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg aagttccccg
    16381 tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt gttaaaacca
    16441 atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag aagcgtattg
    16501 acagcttttt ccaaatggac acggaataat tcattcaaat cgccttttta ctgataaaac
    16561 gataaaatga tagtggttcg tttgttgcat actcatttaa tagatgttga attaaaatat
    16621 agttcttcga ataattactt tttgatttaa catctgcttc accacttact agaatactag
    16681 caaattcttc attcccctca tcccccatta ctcgagacat aatcagaatt ttatctggtt
    16741 taacctcacc tgttaaatca tcttgttccc ataacgcggg ttcaatttgg cttttcaaga
    16801 ataaagcttc cttttcacaa ctattttcta atttagcata gatagctctc aaaaataaaa
    16861 atagcgtagt tatcctttgc tgtccatcaa tgatttcttg ctcgttatga tcattttcat
    16921 aagccacaat gcttcctaaa aagtaagtac tctcgttatt gtttgctgtg tactccgcta
    16981 aatcatcaaa tagtacttgg ttttgctcgt cagtccaggc ataaggacgt tgatattctg
    17041 gaataacgaa ttttttgatt ttccctgttt caagaaactg cttcacgctt tgtttgttca
    17101 cttcaattgt cgttgacata gtttaaatct ccgagacgac actgtttata aaaataaatc
    17161 tatttccttt attctgacat agctgatatg acagagttca gacaatttag tacgtaacaa
    17221 atttttcagg gctttcttaa aagcaaacaa tacaacttct cctttatttt tgagagaaat
    17281 tgtattgtct aatccttatt tataaatttt atttcgtccg taataacttg tataaatctc
    17341 tagcaactaa tgtatatact agatcaaagt acttatcatc atgcccaaac attttctcaa
    17401 aagctgcatt gttcttctcg tactgctcca tcaccttatc tttaaataca tcagggaaca
    17461 gcgatttttc aaacatctga gcatcgttat tcttagccat gctgacaacc ttcaaatctg
    17521 ggttatcggc aaatgaccga tacaaaatct ctactaacac ccggtctcct tcgtcaaaat
    17581 catttggaaa tcgttcattg attttatgaa tgatttcttc tagtgaatct cgttcatccg
    17641 gcggtttgat tcctgcatca attgactttg gatttactag ctcgccttct ttattcacta
    17701 gatggatttc accggaaaag tcctgacgta acttataata ttccaacttt actttgtcag
    17761 aaatatcaat attttctcgt gtttctttag gtagaaactt cagcaagtat ccagtaaaaa
    17821 ttcgttcctc caatagttcc atatcgaata accgatcaag ttgtgaaata taatcatacc
    17881 acttgccaaa gttacgtaga gtcacccgaa actggtactg attatccttg ttcatttcct
    17941 catagcgatc cataatcggc ttaaacatgc tagaaattcg tccaagcaag cgctcattct
    18001 gttgcttttg ggcttgtttc accaatttca tgacacgttc aatatcacct tgattgtaaa
    18061 cattaaattt tcgtagcttg ctttgagtat cataaatcaa gttaacattg atttcttctc
    18121 tcaaatttgt ttgttcataa aagggttgga aagcagcttc aatatcttca acatcattta
    18181 caaaatcaag aataaaggta tccttcttcc caggcattgt tcgattgagt cgtgataaag
    18241 tctgaacagc tttaacaccc tttaacttct tatcgacaaa catggtatgc agcaacggtt
    18301 catcaaaacc cgtttgatat ttttcagcaa caatcaagat attaaaatta tctgtatgga
    18361 aagcttcctt taattgattc tcccgaacat tctcaccaga ttttgtgaca ttcatcttcg
    18421 tttctgtata ttcagtctct ccatctacta ccgaaccaga aaaagcaacc aacacatcca
    18481 agtcattata acctttgttc gctatatatt ttttaaattc tttcatatag cgaacagcgt
    18541 gtaagcgtga tgcggtaacg accattgctt ttgcctgccc accaatagct gtacgagtaa
    18601 ccgctcggaa ttgctcaacc atcacagccg tcttttgagt aactgcccaa ggatgcaatg
    18661 attggaaacg aacaatcgct cgggttgctt cactctttgg taattctggg ttatcttcaa
    18721 tttcacgggc aattttgtag gaagtactat aggtcatata gttcttcagc acatctaaga
    18781 taaagccttc ctcaatcgcc tggcgcatcg agtaaacatg aaagggttcg aaatgcccat
    18841 ctggttgttt cgtaccaaac atttccaatg tcttttcttt cggtgtagcg gtaaaagcaa
    18901 agaaacttaa attttgatgt ttaccttgtg ctaccaattg ttgaactagt tcatcttcag
    18961 catctggtaa tacatcgtca acttcgcctt ccagttcata atactcttcc aaggatttag
    19021 atttgtccga aagagcggct tttagcttct gggcactgtt cccagtttgt gaagaatgtg
    19081 cctcatccac aatcacagca aaattacgac cttccacttg atctacttcc tgatagatca
    19141 ctgggaactt ttgcaaagtg gtaataatga tcttacgccc atcattaatg gcttctttta
    19201 aatcttgaga ggatttgcga tcgtcaatcg tttctaccaa acctgtctga tgatcaaagt
    19261 tcatgatagt attttgcaac tggcgatcca acactgttcg atcagtcaca ataataattg
    19321 atgaaaaaat tgggtagttc agttgatcat gaatagaggc taaatggtat gccagccatg
    19381 caatactatt agattttcca gaaccagcac tatgctggat caaataattt tctccactac
    19441 catggctttt tacatctgca accaattttc gaaccacatc taactgatga taacgaggaa
    19501 aaatcaacgt tttctttgtg acttctctgt cgccttgctt ttctttttta atctctagat
    19561 tcataaaacg ttggataata tccatcaaat tgtctctagt aagcaccttt tcccacaagt
    19621 aggcagttgg gtagccatct ttattggacg gattgcctgc tccaccaact cttcccggcc
    19681 cattggaacc ttgattgaat ggcaagaagt acgttttttt accgtccaac tttgtcgtca
    19741 tataaacatc tgtatggtcc accgcaaaat agactaagaa gcggcgatta aaacgaaata
    19801 ccaattcttt tggatcacga tctttttgaa actggagttt ggcattttct actgtttgac
    19861 cagtgtactg gtttttcaat tctaaagcaa cgagcggaat accatttaac gacaaaacca
    19921 tatcaatggt tttatggtta ttaggactgt aagcaaactg gcgagtacag gtaaaactat
    19981 ttgcttcata cttttccatc gtttcatcat tcagctctga agcaggtttg aagtaagcta
    20041 ctttaaagcg cacaccgcgg tctttgatcc cattgcgcaa tacatgtaac atgccgtggg
    20101 tttctacctc ttgattaaag cgttgaagga acttgacttc tccatcattg ttatagtttc
    20161 gttggtgacg tgcccactgt tttggttgag tcttctggac aaagcctagc aatttttgac
    20221 aatccaaacc aattatggga tcaaagtttg attcattgaa tgtttctttc ttccatttgc
    20281 catacgtagt cagtgaagtc tcaatatcct gctcaaaacg aatttctttg ttctctacca
    20341 aatgaatcac ctcctaggct tcttttttac cggttacgta ttcgtagatg agagcttttt
    20401 tataactctc aagttccatc aattcagcta tcttattatc aatcatttga tttattat
//
LOCUS       DACIFS010000047        20401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.047, whole genome shotgun sequence.
ACCESSION   DACIFS010000047 DACIFS010000000
VERSION     DACIFS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.047"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            135..410
                     /locus_tag="IYA83_001831"
     CDS             135..410
                     /locus_tag="IYA83_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ8587259.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            416..1183
                     /locus_tag="IYA83_001832"
     CDS             416..1183
                     /locus_tag="IYA83_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ8587260.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1351..3180
                     /gene="typA"
                     /locus_tag="IYA83_001833"
     CDS             1351..3180
                     /gene="typA"
                     /locus_tag="IYA83_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ8587261.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IYA83_001834"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IYA83_001834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ8587262.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IYA83_001835"
     CDS             5326..5628
                     /locus_tag="IYA83_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ8587263.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IYA83_001836"
     CDS             5631..6794
                     /locus_tag="IYA83_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ8587264.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IYA83_001837"
     CDS             6820..10248
                     /locus_tag="IYA83_001837"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ8587265.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IYA83_001838"
     CDS             10269..11393
                     /locus_tag="IYA83_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587266.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IYA83_001839"
     CDS             11383..11820
                     /locus_tag="IYA83_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ8587267.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IYA83_001840"
     CDS             11804..12142
                     /locus_tag="IYA83_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ8587268.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IYA83_001841"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IYA83_001841"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ8587269.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IYA83_001842"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IYA83_001842"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ8587270.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IYA83_001843"
     CDS             13299..14342
                     /locus_tag="IYA83_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ8587271.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IYA83_001844"
     CDS             14501..15229
                     /locus_tag="IYA83_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ8587272.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IYA83_001845"
     CDS             15345..15590
                     /locus_tag="IYA83_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ8587273.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IYA83_001846"
     CDS             15587..15805
                     /locus_tag="IYA83_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ8587274.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IYA83_001847"
     CDS             15941..16480
                     /locus_tag="IYA83_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ8587275.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IYA83_001848"
     CDS             16473..18746
                     /locus_tag="IYA83_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ8587276.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IYA83_001849"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IYA83_001849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ8587277.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IYA83_001850"
     CDS             complement(19919..20173)
                     /locus_tag="IYA83_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ8587278.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT         6837 a         3431 c         4358 g         5775 t
ORIGIN      
        1 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
       61 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
      121 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
      181 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
      241 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
      301 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
      361 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
      421 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
      481 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
      541 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
      601 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
      661 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
      721 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
      781 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
      841 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
      901 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
      961 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
     1021 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
     1081 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
     1141 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
     1201 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
     1261 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
     1321 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
     1381 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
     1441 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
     1501 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
     1561 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
     1621 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
     1681 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
     1741 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
     1801 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
     1861 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
     1921 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
     1981 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
     2041 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
     2101 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
     2161 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
     2221 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
     2281 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
     2341 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
     2401 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
     2461 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
     2521 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
     2581 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
     2641 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
     2701 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
     2761 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
     2821 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
     2881 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
     2941 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
     3001 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
     3061 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
     3121 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
     3181 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
     3241 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
     3301 ccgaaagacg tttaaaagac ttttaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttagaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga gggattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 t
//
LOCUS       DACIFS010000048        19303 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.048, whole genome shotgun sequence.
ACCESSION   DACIFS010000048 DACIFS010000000
VERSION     DACIFS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.048"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            237..1628
                     /locus_tag="IYA83_001851"
     CDS             237..1628
                     /locus_tag="IYA83_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ8587279.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1687..2490
                     /locus_tag="IYA83_001852"
     CDS             1687..2490
                     /locus_tag="IYA83_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8587280.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2523..3377
                     /locus_tag="IYA83_001853"
     CDS             2523..3377
                     /locus_tag="IYA83_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ8587281.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3374..4075
                     /locus_tag="IYA83_001854"
     CDS             3374..4075
                     /locus_tag="IYA83_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ8587282.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4083..5150
                     /locus_tag="IYA83_001855"
     CDS             4083..5150
                     /locus_tag="IYA83_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ8587283.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5143..6108
                     /locus_tag="IYA83_001856"
     CDS             5143..6108
                     /locus_tag="IYA83_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8587284.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6153..7127
                     /locus_tag="IYA83_001857"
     CDS             6153..7127
                     /locus_tag="IYA83_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ8587285.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7198..8685
                     /locus_tag="IYA83_001858"
     CDS             7198..8685
                     /locus_tag="IYA83_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ8587286.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYILKK"
     gene            8742..9857
                     /locus_tag="IYA83_001859"
     CDS             8742..9857
                     /locus_tag="IYA83_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ8587287.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9854..11269
                     /locus_tag="IYA83_001860"
     CDS             9854..11269
                     /locus_tag="IYA83_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ8587288.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11302..12249
                     /locus_tag="IYA83_001861"
     CDS             11302..12249
                     /locus_tag="IYA83_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ8587289.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12289..13449)
                     /locus_tag="IYA83_001862"
     CDS             complement(12289..13449)
                     /locus_tag="IYA83_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ8587290.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13614..14789
                     /gene="mltG"
                     /locus_tag="IYA83_001863"
     CDS             13614..14789
                     /gene="mltG"
                     /locus_tag="IYA83_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ8587291.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14925..15404
                     /gene="greA"
                     /locus_tag="IYA83_001864"
     CDS             14925..15404
                     /gene="greA"
                     /locus_tag="IYA83_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ8587292.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15572..16303
                     /locus_tag="IYA83_001865"
     CDS             15572..16303
                     /locus_tag="IYA83_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ8587293.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            16300..17367
                     /locus_tag="IYA83_001866"
     CDS             16300..17367
                     /locus_tag="IYA83_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ8587294.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17374..18006
                     /locus_tag="IYA83_001867"
     CDS             17374..18006
                     /locus_tag="IYA83_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8587295.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18146..18811
                     /locus_tag="IYA83_001868"
     CDS             18146..18811
                     /locus_tag="IYA83_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ8587296.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18839..19132
                     /locus_tag="IYA83_001869"
     CDS             18839..19132
                     /locus_tag="IYA83_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ8587297.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19200..>19303)
                     /locus_tag="IYA83_001870"
     CDS             complement(19200..>19303)
                     /locus_tag="IYA83_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ8587298.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6741 a         2702 c         3713 g         6147 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaaatattca ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtca tctcaaggac
     1561 tagatgaaga tgaaaatatt agttcattag cagaaatcga cttttcacaa gagaaaattt
     1621 atcgctagat tggaaaaatt ttgagcaaag aggtaggaga gaacattgga aaataaaaat
     1681 gtaagtatgc tgctttcttt atatgataaa gaaaagccag agtatttaaa ggaagcatta
     1741 actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta tgacggccct
     1801 ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat tggattagaa
     1921 catacgaaaa atcgcttagt agccagaatg gatacagatg atataatgga accccaacga
     1981 ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt tgggagtaat
     2041 attgaggaat ttactggcac gcttagcaat gttatcggaa aaagaattgt acctgaatat
     2101 aacaatgcaa ttcgtgtatt ttctaaaaga agaaatccct ttaatcatat gaccgttatg
     2161 tttgataaaa aagctatttt ggaagtggga aattaccaac cattaagtgg gtttgaagat
     2221 tattatcttt gggcaagatt attaaaggca ggttataaag gatataatat tcaagaaaca
     2281 ttagtctatg cgcgaactgg caaagatatg tatgcaagaa gaggcggaat gaaatatctt
     2341 ggttctggtc ttatgggtcg ttatcgtatc tggaaagacg gtttagggtc agtaaaagat
     2401 tttataattg tttcttgcgg acatattttt attagcttac taccaaatca attaagagga
     2461 aaattttatc aaacgcaatt aagaaaatag gaggtttttc tttgtatagc actgatccaa
     2521 aaatgaaaaa aatacaacag attacgttga aaatgtctca agagattgtt gatttttgtc
     2581 taaaaaatca acttctttgt tatttctgcg gtggtggtgc catcggtact gtgagagaac
     2641 aagggtttat tccatgggat gatgatttag atttctttat gcctagagag gattatgaaa
     2701 aattttatca actatgggtt aattcagaaa aaagtaaaaa atatcccata cagaaggctt
     2761 ctaaaaatta taatgatcat aattctttta caactatccg cgatgcagat actacattta
     2821 ttaaaactta tcaaagagat ttagatatag tccatggaat tactattgat attttcccgt
     2881 tagatgttgc ccctgtttcg aaatttagcc gtaaaattca aaaactatgg gctttagtat
     2941 atgctttatt ttgttctcaa gtcgtccctg aaaaacatgg tggaataatg gctacaggta
     3001 gtaagatact cttatctatt ttccgctcac ctcgtacgag atataagata tggtcttttg
     3061 ctgaaaaaca aatgacaaaa tataataatg aaccaacggg ctatgtgact gaactttgtg
     3121 tcggacctaa atacatggga aatgtttatc ctgcaaaaga ctttgaatct gctgtgtggg
     3181 tgccattcga agatacagag atgccagtac cagttggtta tgataattat ttaagtcaag
     3241 tttttggtga ctatatgcag cttccgcctg aaaaagatca ggtttctcat catgaagcag
     3301 tttatattga tccagaacat ccatacacaa tgtacaaagg aaaatattat ttaactaaag
     3361 gagcagaaaa aaaatgatta cagctattat tattgctggt ggtgtgggta aacgaatggg
     3421 acaagatatc ccaaaacagt tcattacaat tgaaggaaaa cctattatta tctatacttt
     3481 agaatcattt caacaccatc ctcaagttga ccggatttta gtcgtttgca aatctggttg
     3541 ggaagaaacg atgtgggcgt atatcaaaga attcaatatt agcaaagtca aatgggtcat
     3601 tacaggtgga tcaaagggac aagagtcgat caataatggt gttcagtttt tgaaagacta
     3661 ttctgaagat gatgatacga tcatcatcca tgatggaatc cgtccactag tagacgaact
     3721 ggtattgtca gatgtaatcg ttaaatgtaa agaatttggt aacggtgtaa cctctctacc
     3781 ttacaacgaa caaattttta tcaagaaaac agaagaaacg acggaacagt atgtcgatcg
     3841 taacacattg aggcgtgtct caactccaca agcttatcaa tacggaaaac tacgggcagc
     3901 atatgaccgg gcagtgaaag aagacatcgg tatgactgat tcttcttata cgaataccat
     3961 gatggtagat ttgggagaaa ctttatattt tgctgctggt tctgataaaa atatcaaatt
     4021 gacaacaaca gatgatctag aattgttcaa agcatattta aaaatgaaag actaggtgaa
     4081 agatgatgac gttgattaaa caaccaatgt accaagaaga tttggcaaaa gtaatcaatc
     4141 atacaccaaa aataaaacag ctacatggaa aatccgttct gatgattggt gcttctggaa
     4201 tgatcggctc ttttttgatt gatacgttga tggctgctaa tgaacaacta ggcatcaaaa
     4261 tcaaagttta tgcaatgggc agaaaccgtc agaagcttga aaaaaggttt gccagttatc
     4321 ttgaattaga tactttcgaa attgttgaag gtgacgtcac agaaccctta ccaaaagaaa
     4381 ttcaagctga ctatttgata cacggtgcaa gtaacacaca tcctaaggct tacgcaactg
     4441 atccaatcgg tacgatcatg actaatcttg caggtacaga gcaagtattg aaacatgcag
     4501 tagcaaccca accggttaga gtattatttt tatcaacagt agaaatctat ggcgaaaatc
     4561 gaggcgatat agaaaaattc acagaagatt actgtggata catcgattgt aatacactga
     4621 gagcaggcta ccctgaagga aaaagagtca gtgaatcatt atgccaagcc tacatcgcaa
     4681 aacacgacct cgacatcgtg attccaaggc tttgccgtac gtttggtccg acgatgcttt
     4741 tatctgatac gaaagcatcc tcacaattta ttttaaatgc agtcgaacaa aaagatattg
     4801 ttttaaaaag tgaaggaaac caatattttt cgtatgtata tgttggagat gctgtttctg
     4861 ctattcttca tttactgctt gaaggagaaa aaggagaggc gtacaacgtt tcttcagaaa
     4921 actttgattt acgactaaaa gaattagccc aacagctagc tgatatcagc ggcaaacaag
     4981 taatttttga attgccggat gagattgaac aaaaaggttt ctcaaaagct agcacagcag
     5041 tacttgaaaa taaaaaaatt aaagagacag gttggaaacc aatttttcct ttgaacgaat
     5101 cattggttca tacagtagaa ttagttaaag gtgagaaata taatgtgtga gattagtatt
     5161 attgttcctg tgtataaagt tgaaaaatat ttaagaaaat gtgtagattc gattttagcc
     5221 caaacattta ctgattttga agttatatta gtagatgatg gatctcctga caatagcggg
     5281 aaaatctgtg atgagtatgc tgaaaaagat aatcgagtac gtgtgatcca taaagaaaac
     5341 ggcggattaa gcagtgctag aaatgcgggg attgatgttg cgagaggcaa atacttaggc
     5401 tttgttgaca gtgatgatta tattgatgaa gatatgtatg aaattttgta tgagaatttg
     5461 aaaattcatg atgccgacat tagctctgtt gaattgattc ctttttatgg tgatagatat
     5521 aagaaagcta ataaagaaaa gaaagtaatt atattaaata aaaaggaagc aataaaatct
     5581 gttttagaag gaacgcaatt ttatgcatac gcatggaata aactttatcg taaggaactt
     5641 tttaaagata atcgttactt ggatggaaaa acatttgagg atgcatatat aataatagat
     5701 ctattatttc aaacagaaaa aattgttgtt tccaatgaag agaaatattt ctatcttcaa
     5761 agaaatgata gtattatggg aaaaagtttt tctatgaata atttcgatgt tatagaagca
     5821 tggcaatata acaaagagaa gattttagat gcttttccag atttgcatga ttcctattat
     5881 cgccgactat gttgggcata tttttacgtg ttggataaaa tggttttatc aagtagttat
     5941 caacaaattc cacgtaaaaa aacagtaatt gaatttttaa agaagaacag aaattttata
     6001 ttaacttata agggattcac taaatcgcgc aaaatagcta ttattgctct gagctttaga
     6061 gtagattggt ataggaaatt ggttgaacaa aaaaataaaa aaaattagaa tgtttaaatt
     6121 agtaaataaa agaaaaggaa ttgaaaacta aaatgtgcag aataagtata atagttccag
     6181 tttataatgt tgaaaaatat ttaaaaaagt gtgtcgattc aattctaggc caaacattta
     6241 ccgattttga attattatta attgatgatg gttctacaga taattcagga agtatatgcg
     6301 atgaacttgc gaaaacagac aaccgaataa aagtgataca taaggaaaat gggggtctaa
     6361 gtgatgccag aaatattggt attgaggtag caaagggaga ttttattggg tttatagata
     6421 gtgatgatta cattgatgaa gatatgtatg catttttata taacaatata ttgaaatatg
     6481 atgcagagtt atcaatgtgt ggtatttatg acgtccataa gaataaagaa attaaaaagt
     6541 taactccttt ttctcaattg gttactaaat ctgaagcaat tgaattagta ttagatggta
     6601 agctagttgt tgctaatgca gtaagcaagc tatataaaaa agaattattt gagaatgtga
     6661 gatatccaaa aggaaaaatt gctgaagacg cagcagtaat actgaaaatt atcaatcagt
     6721 gtaataaaat ccatgttgat acttctcaaa aatattatta ttatcatcgt ggagatagta
     6781 ttactagtaa aaaatttaca aaaaaagatt ttgatataat tgatgtatgg aatgagaatg
     6841 aaaaatggat tattaaaaat tatcctcaaa tgtacgataa agtacatact agagtttgtt
     6901 gggcttactt tgttgtgtta gataaattag ttttaactga agaaaaagga ctgtatcaag
     6961 aaaaaaaaca agtaagagag tatttaattg ataatttctt ttttattata aaaaataaac
     7021 gattaacttt tcaaagaaaa atatctatgt tattgcttac ttttggaatt gctgcgtata
     7081 aaataccggt taaatatcag tataagaaag taaaggctgt taattaatgc tttagttagg
     7141 atgaattaga tgaaaaaaag caggttagag tattctttaa taaattcttc tatatcaatg
     7201 tttatatata ttttaaggat agtaatacaa tttgtaggta gaagtttttt tatttatttc
     7261 ttaggagcga aatatttagg attaaatggt ttatttacaa atatacttag tttcttgtcg
     7321 ttggctgaac taggcatagg ctcttctatt atttattccc tatacaaacc attagcccaa
     7381 aaggatgaaa gtaaagtaaa ggctttaatg aatttgtata agaagacata tgaatttatt
     7441 ggtattttta tcggcatagt aggagtattg attattccgc tgttacctct atttattaaa
     7501 aatagcacag acataccaca tatgtatcga tattatgtat tatttttatt aaattcagtt
     7561 cttagttatt ttttcacgta taaaagatca cttgcaatag cagatcaaaa aaattatctg
     7621 gttgcgatta atgattttgc ctttttattc tttatgaaca ttgtacaagt aattgctttg
     7681 tacttttatt ctaattttac tctatttcta atagtgcaga ttttgtttac tctattttct
     7741 aatataaata tttccagaac agttgacaag agatattcat atttaaaaga gaaagatgta
     7801 gtttctctag ataagcaaac aaaagatgaa gtaaagaaaa atgtcatagg taatatgtcc
     7861 tcaaaaatag gcggggttat tgtaatgggg acagataata tattaatttc tacatttgtt
     7921 aatttaacag ctgtaggaat ttattctaac tattcattga ttgtcacaag tgtgcaaaat
     7981 ttatgtaagc aaattactaa ttctatcacg gcaagtgtag gaaattttgc tgtatcaaat
     8041 aaaaaaaagg attcatttga gctatttaaa aaacattttt ttatcaacca ctcactaatc
     8101 tttttttctt ctatattgtt attgacttta atgaatccct tcattagctt gtgggtagga
     8161 gccaaataca tgctacctat gtcaacgact attttaattg ttttaaatta tagtgtacag
     8221 gtttatagaa atacatcctt cgtttttata gagtcatttg gtttatattg gtctcaacgg
     8281 aaaaagccta taattgaagc aggtgtaaac ttgttattat ctttgctttt gttaatagtt
     8341 tttgatatgg gaatcaatgg agtacttata gggactatcg gatcgtctct aggatttgtg
     8401 atttggtatg aagcatttat catatataaa acggtatttg agaaacgttt tattcatttc
     8461 tttttaactt tctggcgaag ttttatagaa ttaataattt cggcaatagt cgtttttcta
     8521 ttaaatgaat atttagcaaa tggaggcagt acgttccttc aatttataac taaaacctta
     8581 ataactatag taacaacatc gatattatat ttgttattat attttagaag agaagaattt
     8641 acatttatca aaaaaattct ttttaggtat attttaaaaa aatagctttc tataaaggag
     8701 tgttttattt gaaaatagct atactatctc cagctttctc aatgggagga gctagtatcg
     8761 tggcctcaaa aattgcaaaa ggattaatag atagtggcca tgaagttttt tttgtttctt
     8821 acttagaaag tgaaaaacca caagtagaat ctaagtatta tgatttatac gaaaaaaaaa
     8881 tttttttatt taaaaatttt tctcgatttc ataagatatt agagtataga ttgtttggag
     8941 gctttgttcc tagtagatat ataaaaaaag aaaaagaaca actaaaaaaa gttctaaaga
     9001 aagaaaaacc tgatgtcatc atattcaata cttttatacc tagtgtacta ttctccgaat
     9061 tcctaaagta tgaatttcca acagtgaaat taattacttg gatgcactct gatcctgatt
     9121 acagcttaaa tcatattgct aaattttata aaaaagaata tagaaaaagt tttgaacgcg
     9181 tggattccat tgtttgtctt tcgcaagata caaaagaaaa actttcatta tacaataacc
     9241 atattaaagt tatttataac ccattgacat taagcggctc tagtttaata agtaaccttg
     9301 ataaaaaaat tatttcattt actgccagat acgatataaa tattaaaggg tttgattatt
     9361 tatgtgaagt agctaaaaac ttacctgttg gatgggctat aaggattgct ggagatggta
     9421 cagaagaaga acgtaaagag ctgaaaagat taattgagaa aaatggtgtg ttagataaaa
     9481 ttatttgcgc aggtgcatta tttggagatg aattaatcaa tcattatata gaaagttcta
     9541 ttttcatatc tacttctaga agtgagggac taccattagt gttgatagaa gcaatgactt
     9601 atggattacc tgtaataagt tttgatcatg gtggtgctaa ggaaatatta caagatggaa
     9661 attatggaat tttaattaag aattttgacg tacaagatat gaataatgag atagaaaaat
     9721 tgtctaataa tcgtttactt atggaaaaat atcaaaaatt ctctttagaa agagctaagg
     9781 attttaatga aaaaaaaata attggtgaat ggcaaacatt gttaaatgaa ttaggaaatg
     9841 aggatgttag aaattgagga cttcgattaa aaattttaat ttatggaaag aatggtatct
     9901 atatatattt tttataggga tgattattcg tgcaataaat ttttatagtt tattaccgtc
     9961 cacttttgat agtatttttt ttaaacttat aggaatttca ggagttttgc tattagggat
    10021 agatttcata attaaattta ataaaagaaa agtaacttat aacattttat tgatagcata
    10081 tatggtgatt ttagtaataa cgagttttgt tcataaagaa tatggtctag gagaaaactt
    10141 aaagactata atgtgggcaa tcttacagta ttttttgatt tatcagtttg cttttgagaa
    10201 tagcgataat aaacgatttt ttaaaaggat aagttgggga tttatttata gttggtttgt
    10261 tttagtatca atgtcaatgg ttcttttctt tggtaaattc ggctatgaga aatattataa
    10321 tgctagacat aggatcagaa ttggtttttt agaatcaaga ctgtttggga cgttttctga
    10381 tattaacaat gctgctatag catctttagt tgttattgtc ctaatcttat tttatcttta
    10441 taataaaaaa gtgacaggat taaataaaat cattttttta attaatcttc cattacaatt
    10501 tatttatata gtattaagtg ggtcacgatc tacctatgtg gtaagtataa taatagttgc
    10561 catatcatct tttattgttg tgttaaaacg aaatggaaca aaaaaagatt taaaaaacta
    10621 tatccttgct atagtatctt ctattggttc tgttctgatt ttattcgctt tgtttaatgc
    10681 aactaagata ctttttcaag aattattacc tcatttaaaa gaaataaatt tttttaattt
    10741 tacagagcgt cataaagaat taatgaataa tagtcaacat attaataata ctcaaacacc
    10801 tgataatact cttgttagga aagacgtagc tgaaaataca gatatttcta atgctagaat
    10861 tactatatgg aaaagtgcat gggaaatatt taaaacaagt tggcttatcg gagtctctcc
    10921 gaaaaatgta gtatcttatg cacagtcaat tattccagat ggatatattg cgaaagtagg
    10981 aattgccatt cataatgcat acttgaatgt attaacatcg acaggtattc taggaataat
    11041 tcctttcatg gcttttttaa taaagagttt tgtgctgaat gtttatcgaa tcatctataa
    11101 taaatgcatt ggtaaagatt atcattttgt ttactgttta gttattttat cttatgcact
    11161 atacgcagct cttaacaatg aattagtcta tgaaaacact gtaggaacgt ttgttttttg
    11221 gctattttta ggaagagtta atagcaaaaa taattctagc tatttttagt aataaatttt
    11281 atcttgaaaa gaggagaaaa aatgttgaat tacttgatta ccggtggcgc cggcttcatt
    11341 ggctcgacgc tcgcgaatta tctaggaaaa gaaaacaatg tagttgtgat cgatgattta
    11401 tcgatgggga aaaaagagaa tctggatgaa tcgaagcaca tcacgtttat tgaaggggat
    11461 gtcgctgaca aaaatttgat ggaaaaggtc atgaaagaat accatttcga ttatattttc
    11521 catctagctg ctgtagctag tgtggcagat tctgtagaac gtcctctaga aactcatcgt
    11581 gtaaattttg acagtgcgct tttattgctt gaattggtac gaaaatatca atctggttta
    11641 aaacgattgg tatttagttc atcagctgca gtttatggcg atgaaccaac gttgccaaaa
    11701 aaagaagaat cggtcatccg tcctttaaca ccatatgcca tcgataagtt tgcagcagaa
    11761 caatatgttt tggattactg tcatttatat gatgttccga ctagtgctgt ccgatttttt
    11821 aacgtttatg gtccaaatca aaatcctaac tctccatata gcggagttat ttcgattcta
    11881 gtggaccgat acaaaaaaca attggcagga gaaaaaacag aatttacttt atttggtgat
    11941 ggtagccaat ctagagactt cgtttatata gaagatgtta tacaagcact tttacttgtt
    12001 gctaaggaag aaaaagcttt aggacagcaa ttcaatgtcg gtacaggaaa aagcactacg
    12061 ctacttgaat tgattcattc aattgatcaa atcttaggaa cagagctagc gcttaaatat
    12121 gaagcggagc gttcagggga tattcgtgat tctttagcgg atatttcaaa gatccgttct
    12181 ttaggttatc aacccaaatt tgatatattg aacggaatgg aacgttattt aaagacagaa
    12241 atcgattgaa acaaaaaacc agcatcctgc tggttttttt gtctcgactt agctttttaa
    12301 attaacacct tccatcagtt cgtaaatctg atttcttaaa tcagtggccg gcaatttgat
    12361 gtttttatca gattggatat tactttccat gacgattgca tcttttgtgt cttttttcat
    12421 cgccgctact gcatggaatc gattgatatt tccctgagca aggatataat catttccact
    12481 gtaaacaccg ccgctgtaag gatgtttatc accattattt tgccacaatg agaaaagtag
    12541 ttcttttggt ataatatcgc cagacagaac actggtaaag aatttgttca agtcataaac
    12601 agacatactt atatttcctg tgcctaattc atttcggatg tctgtttcag gctcgaccaa
    12661 tgcttttgaa taatcgtcag ttgcagacat ttggtaagat acagcatgct gaggggaatt
    12721 ttttacattt tcataaaaat ctgtatgttc tagatggagt ggccgaatga tttctttttt
    12781 taaaagagtt tcataaggct gattcattag tttgatcaat acccctgtaa gcaatgtata
    12841 atttaatggt tcataatgat acgtatcata accagtaaat ttcaaatgat gaagggcaaa
    12901 ctgaatgatc tcactatctg acataggttt ttttggagaa gcggttcgat gaagaccaga
    12961 gcgcatgtaa agcatatttt tgattgagat ttgctgactt ccaggaatct gaggataaaa
    13021 ttcagatagc tttgtgtcaa aagttatctt gccagattga atttgtttta ggataagtaa
    13081 agcagtcatc gctttttgga tcgaaccgat ttgatagtaa agagaaggag aattcgattc
    13141 ttttatgcgt agatttgcgt acccatatcc tttttctaat atcactcgat tatttttgcg
    13201 tacataaatc gaaccgatat aatctgattt ttgcagtaat tgatcaattt tgctggcaag
    13261 catctgctca ttagatgtta aattggaagc aagttgtgcg ggttccgttt tataaatttg
    13321 tggttgaggt tgttttgtta attgccatcc tgtaatgatg cctgttgaaa agaagctgac
    13381 agccagcaac aaaactgcga gaaaagaaag agagatctgc tgtttttttt gatgttttcc
    13441 ttttgccata ttaaatcctt cctatccatt tttaactatt tttcattgta atcctcaaaa
    13501 agagcatttg ccacttacaa ttttgacata atatttggta aaataattca cggcagtaga
    13561 aatttcatgt ataataaaca gagttacgaa gatgatagta aggaggagtt gttttgtcag
    13621 gaaacaaaca acacccaagc aaagacaaaa aagaagccag taaggcagct acagcagata
    13681 taatcgacaa gcggagaaat ctgcgtaaaa aagaagataa gatcgtgagg aagatcattt
    13741 tagtgatcgc tcttacatta ttgattattg gcggatttct cgggtttacc gtatatcgtt
    13801 atgtagatag cgggttgaag cctttagata aatcggacga ccaattagtc caagtggaaa
    13861 tcccaagcgg ctcttctaat aagcagattg gcgaaatttt ggagaaagac aatattatta
    13921 aaagtgggat tgttttcaat tattatacaa aatttaaaaa cttaactggc tttcaagctg
    13981 gttattatca gttagcgcct aatatgactc ttgatgagat tggcaagcag ttgcaagaag
    14041 gcggcacatc tgagccaaca aaagttgcag atgggaaaat tgccattcca gaaggctacg
    14101 acatcgatca gattgctgag cgggtagcga aggtaactgg taaagacaag aaagaatttt
    14161 tagatttagt aaatgatgag actttcttta acgagttgta tcaaaaattc ccagaattgt
    14221 tggaaagtgc ctctaaagct gagaacacaa aataccgttt ggaaggctac ttattcccag
    14281 ctacttatga ttactacacg agtacttcgc ttaaggatct tgtgattgag atggtgaaca
    14341 aaacaaatac ggtcatgcaa aactattatt cagctatcaa acagaaaaat ttgactgtac
    14401 aagaagtttt aactttggct tctctagttg aaaaagaagg cgtaaaagaa aatgatcgta
    14461 aaaatattgc acaagtcttc tttaaccgaa tcaaggcaaa tatgccactt cagtcagata
    14521 tctctgtttt atatgcatta ggagaacata aagaattggt gacgtatgaa gatacagctg
    14581 tagattcacc atacaatttg tataccaata ccggctatgg gcctggtcct tttgacaatc
    14641 caagcgaaga ggcaatcaag gcagtattag aaccagcaga aaatgactat tattatttcg
    14701 tagcagatac gagcactgga aatgtttatt tcgctaaaac gtatgaagaa catatggaac
    14761 tagtgcaaaa atatgtaaat aattcttaga aaaacagggg ctagtcattg atcattttta
    14821 gaaaaacatt tacattttaa gaaaagcgtg gtaatctttt ttggactata ttcctcaaaa
    14881 gattaccttt tgcggtattt tatcgaaaaa aggagacgtt acacatggta gagaaagttt
    14941 atccaatgac tctagaaggt aaagaaaaat tagaaaaaga attagaagaa ctaaaaacag
    15001 ttaaacgcgg agaaatcatt gaacgaatca aaatcgctcg cggtttcgga gatctttccg
    15061 aaaactccga atacgaatca gctaaagatg aacaagcatt tgttgaagga agaatcacaa
    15121 caattgaaaa tatgatccgt tttgcacaaa tcatcgataa tgacggcgta gatgcagatg
    15181 aggtatcgat cggtaaaaca gttacgttca tcgagttgcc tgatggagat gaagaagagt
    15241 acacgattgt cggaagtgct gaagcagacc cgttttctgg aaagatttcc aatgattctc
    15301 ctatcgcgca agcattgatt ggcaaacgta aaaatgatga agtaaccatt gctacaccag
    15361 gtggcgatat gttagttaaa atcgtcaaag tagaacaagc gtagtctgac tgaaagatct
    15421 agagagttca tcttactgat gacgcactct agatcttttc tctttgagta ttgcaggttt
    15481 cactgaacga tcttacatct aaagtcctat agccaacagt caaaaaaata ggtatgatga
    15541 aatgagaatg tgtcaggagg gagaaaaagc gatgaatagt tcttggcgtt tttttgtggt
    15601 agtcgaagca ctgctgctgt tatttgctgt ttggcaaatc gtaaacaata ctggactttt
    15661 gcttttagta atattcggta ttttcaatat atatctagct atgcgcaaat atcctcgtac
    15721 taaatttcaa aattttcaat tgatcttagg gagcttggtc atttttttta gcttagtcaa
    15781 cagtcctgct ttatggatga tggttgtatt ggccgtttta tttatcgggc tgaaaggtgt
    15841 agagatttca ggaatagatt tgacaaaaaa tgctttttgg cgaaaaaaac agatcatgat
    15901 ggtccaaaca gagcaattaa aaacacataa taatgagcgt aaaagacagc aattatttgg
    15961 taaccaacgt attggaaacg acgtctatga atgggatgat atcaacattg ccatcatttc
    16021 tggtgatact atcatcgatt tgggcaatac acttttgcca aaagatgata atattgtgat
    16081 tgtaagaaaa gggataggca gaacacggat cttagtaccc ctgggagtgg ctattcgttt
    16141 agagcatgcg acattagtag gaaatgtctt gtttgaagaa gagcagtttt ccttaaaaaa
    16201 cgaacagatc aaaatctgca gcagtgatta tgatgaaaat cctcgcagat tgaagatcat
    16261 taccaatact ttggttggag atatcgaggt gatacgaata tgatgggaag aatatccaaa
    16321 gcaatgctag ctgtttattc ggggattgcg gctttcctta ttattctatt ttcacttttc
    16381 acttattttt atgccagtaa ccaaagtcat tggtggggag aattgctgcg tgcgcgttta
    16441 ttatacgttc cgcttatctt ccatttgatg gcaatttcat taggtgtagg attgatcgta
    16501 tttctattgt tatcacttat tcaaaaggca aaatacggga aaatcgaaga aaagcttcgt
    16561 gcactttctt ctggcaatta tgaatccaaa cttttgcatc agccgatccc aagcgcgtcg
    16621 gatgatctgt acatcaaaga tattgatatg gaaattacta aaataaaaga aaaaatgatc
    16681 gaagtatcca gtgaattaca gattatgact agccgtcctc aatatgtaga tggacaaacc
    16741 aaagaagaga ttttaaaatt agaaaggcat cgattagctc gtgagcttca tgattcagtt
    16801 tcacagcaat tatttgcagc gatgatgatg atgtcggcat tgacggagca agcagaaaaa
    16861 agtgagacac cagaaatgtt ccgtaagcag ttgaaaatgg tggcagagat catcaacgct
    16921 tcccagtctg agatgcgtgc gctgcttctt catctgcgtc cagtcaattt agaagaaaaa
    16981 agtttaaaac aaggcattga gcagttattg aaggaattgc agaataaaat ccagatttcg
    17041 ctaaaatggg aggtagaaga tgtaaaatta actagttcca ttgaggatca cttgttccga
    17101 atcgttcaag aattgttatc aaacacatta agacatgcta aggctaatga attagaagtc
    17161 tatttgcata aaatagataa caatctttta ttacgtatca ttgacgacgg gacaggattt
    17221 aatatgaatg aaaccaaaac gggaagttat ggattgaaca atatcaaaga acgagtagct
    17281 ggtattggtg gaacagtaaa aatcattagt tttaaagggc aaggtacgag cgtggagacc
    17341 aaggtccctt tgatgaagga ggcatagata aagatgataa aagttttatt agtagatgac
    17401 catgaaatgg tacgcttagg cgtctcttct tatttatcta tccaagaaga cattgaagtg
    17461 attggagaag ctgaaaatgg acgtcaaggc tatgaaaagg cgatggcact tcgaccggat
    17521 gtcattttaa tggatctagt catggaagaa atggatggca tcgaatcaac aaaagcaatc
    17581 ttaaaagatt ggccaaaagc caaaatcatt atcgtcacaa gttttattga tgatgaaaaa
    17641 gtttatcctg caattgaagc aggagcagca ggatatttgt tgaagacgtc tacagcacat
    17701 gaaatagctg atgctataag ggctacgcaa cgcggcgagc gagtactaga acctgaagtg
    17761 acaacaaaaa tgatggaaaa aatgagtcgg cgaaatgatc cagtattgca tgaagaatta
    17821 acgaatcgag aaaatgaaat tttgatgctg atttctgaag ggaaaagtaa tcaggaaatc
    17881 gcggatgaat tgtttatcac actgaaaaca gtcaaaacgc atgtgtcgaa tatattagct
    17941 aagttagagg tagaagaccg aactcaagca gctatctatg cgtttaaaca tggtttggtg
    18001 aaataaaaac gatgataaag aggataatca aaagctgaga aaataactat tatttttctt
    18061 ggctttttta caataatgat gcttgctttt gtttttggaa aggctttgtt atactgagaa
    18121 aagaagatac taaaggagga aaatggtgaa acaaaatttt gcaattgtag gccttggacg
    18181 atttggcgga agcatctgcc gtacactgat cgaagctgga caagaagtac tggctattga
    18241 cagtaatgaa gatcgagtca atgaatatat gaatatagcc acccatgctg ttgtggcaaa
    18301 tgcacaggat gagatgacac ttcgttcatt aggtataaga aatttcgatc atgtaattgt
    18361 cgctattgga gaagatatcc aagccagcat cttagttact ctgatggtaa aggaaatggg
    18421 cgtaccgaat attttagcaa aagcccaaaa tgagtaccat gcccgtgtac tagagaagat
    18481 cggagcagat cgtgtcgtac accctgagcg agacatgggg atacggattg cacataactt
    18541 agtatcaaag aatattctag actacttaga gctgtcagat aaatattctt tagcagaaat
    18601 ccgagtctcc aatcctaaat ttttcaacaa aacattagct gaattaaatt tcaggcaacg
    18661 ttttgactta acagtagtcg ccatacgaag agtagatcaa acggttatcg cttctcctgc
    18721 agcagatgaa tatgttcgtg aaaatgataa tctactggtt attggagaga caggcgatgt
    18781 ggatattcta gacgataaga tgaatcaata gaaagaaaag agaaagtagg ttgttgggat
    18841 gaaattaacg gtaacgccaa aagcacaaaa gtggtttgac tcagaaattg atctgccaga
    18901 aacttacgga atacgttttt ttggaaaagt ctacggcaaa acagaggttc atgatggttt
    18961 ttcgattgga atgtcagttg aacagccaga acatcctgta aagaaagtgg aaatggatgg
    19021 gacacttttt tttatcgagg aagcagatga atggttcttt aaaggatatg atttacttgt
    19081 tgattatgat cccaacctgg aggagccaac ctatcacttt aaaaaacaat aaaaaaagag
    19141 agggtgacac aatttttgtc accttctctt ttttcttttg aaaatattgt tttcagctat
    19201 taatatcctg cagacaattt tacttgattg gtttgaagct catgggtggc aaagtagctt
    19261 ttcagattgg ttaaaaaaat agccattaat ttcttttgaa aat
//
LOCUS       DACIFS010000049        18896 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.049, whole genome shotgun sequence.
ACCESSION   DACIFS010000049 DACIFS010000000
VERSION     DACIFS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.049"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(22..252)
                     /locus_tag="IYA83_001871"
     CDS             complement(22..252)
                     /locus_tag="IYA83_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587299.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="IYA83_001872"
     CDS             complement(688..1254)
                     /locus_tag="IYA83_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ8587300.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="IYA83_001873"
     CDS             complement(1254..2210)
                     /locus_tag="IYA83_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ8587301.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="IYA83_001874"
     CDS             complement(2210..2866)
                     /locus_tag="IYA83_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ8587302.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="IYA83_001875"
     CDS             3236..5254
                     /locus_tag="IYA83_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ8587303.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5681..8095
                     /locus_tag="IYA83_001876"
     CDS             5681..8095
                     /locus_tag="IYA83_001876"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ8587304.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="IYA83_001877"
     CDS             8443..9996
                     /locus_tag="IYA83_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ8587305.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="IYA83_001878"
     CDS             complement(10377..12161)
                     /locus_tag="IYA83_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587306.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="IYA83_001879"
     CDS             complement(12176..13897)
                     /locus_tag="IYA83_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587307.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="IYA83_001880"
     CDS             13997..14758
                     /locus_tag="IYA83_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8587308.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15725..15993
                     /locus_tag="IYA83_001881"
                     /pseudo
     CDS             15725..15993
                     /locus_tag="IYA83_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16665..>18896
                     /locus_tag="IYA83_001882"
     CDS             16665..>18896
                     /locus_tag="IYA83_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587309.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5552 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    15301 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    15361 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    15421 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    15481 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    15541 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    15601 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    15661 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    15721 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    15781 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    15841 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    15901 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    15961 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    16021 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    16081 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    16141 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    16201 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    16261 gatgaaggtt aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc
    16321 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    16381 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    16441 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    16501 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    16561 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    16621 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    16681 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    16741 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    16801 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    16861 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    16921 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    16981 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    17041 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    17101 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    17161 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    17221 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    17281 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    17341 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    17401 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    17461 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    17521 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    17581 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    17641 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    17701 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    17761 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    17821 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    17881 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    17941 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    18001 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    18061 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    18121 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    18181 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    18241 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    18301 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    18361 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    18421 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    18481 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    18541 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    18601 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    18661 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    18721 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    18781 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    18841 ttcaaggagc tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DACIFS010000050        18504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.050, whole genome shotgun sequence.
ACCESSION   DACIFS010000050 DACIFS010000000
VERSION     DACIFS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.050"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            99..746
                     /locus_tag="IYA83_001883"
     CDS             99..746
                     /locus_tag="IYA83_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ8587310.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IYA83_001884"
     CDS             1139..3784
                     /locus_tag="IYA83_001884"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ8587311.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IYA83_001885"
     CDS             4094..5407
                     /locus_tag="IYA83_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ8587312.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IYA83_001886"
     CDS             5397..6050
                     /locus_tag="IYA83_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ8587313.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IYA83_001887"
     CDS             6105..6797
                     /locus_tag="IYA83_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ8587314.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IYA83_001888"
     CDS             6947..7147
                     /locus_tag="IYA83_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ8587315.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IYA83_001889"
     CDS             complement(7401..9080)
                     /locus_tag="IYA83_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ8587316.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IYA83_001890"
     CDS             complement(9082..9294)
                     /locus_tag="IYA83_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ8587317.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IYA83_001891"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IYA83_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ8587318.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IYA83_001892"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IYA83_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ8587319.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IYA83_001893"
     CDS             13269..13466
                     /locus_tag="IYA83_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587320.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IYA83_001894"
     CDS             13619..14104
                     /locus_tag="IYA83_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ8587321.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IYA83_001895"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IYA83_001895"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ8587322.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IYA83_001896"
     CDS             14981..15508
                     /locus_tag="IYA83_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ8587323.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IYA83_001897"
     CDS             15599..16336
                     /locus_tag="IYA83_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ8587324.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IYA83_001898"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IYA83_001898"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ8587325.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IYA83_001899"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IYA83_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ8587326.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACIFS010000051        17963 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.051, whole genome shotgun sequence.
ACCESSION   DACIFS010000051 DACIFS010000000
VERSION     DACIFS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.051"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            252..815
                     /locus_tag="IYA83_001900"
     CDS             252..815
                     /locus_tag="IYA83_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587327.1"
                     /translation="MEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVL
                     LAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKK
                     NGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGER
                     YYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            895..1869
                     /locus_tag="IYA83_001901"
     CDS             895..1869
                     /locus_tag="IYA83_001901"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ8587328.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2058..3194
                     /locus_tag="IYA83_001902"
     CDS             2058..3194
                     /locus_tag="IYA83_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ8587329.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3331..3675
                     /locus_tag="IYA83_001903"
     CDS             3331..3675
                     /locus_tag="IYA83_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ8587330.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3946..5232
                     /gene="efmA"
                     /locus_tag="IYA83_001904"
     CDS             3946..5232
                     /gene="efmA"
                     /locus_tag="IYA83_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ8587331.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5370..5969)
                     /locus_tag="IYA83_001905"
     CDS             complement(5370..5969)
                     /locus_tag="IYA83_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ8587332.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6373..7494
                     /gene="mnmA"
                     /locus_tag="IYA83_001906"
     CDS             6373..7494
                     /gene="mnmA"
                     /locus_tag="IYA83_001906"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ8587333.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7740..9161
                     /gene="arcD"
                     /locus_tag="IYA83_001907"
     CDS             7740..9161
                     /gene="arcD"
                     /locus_tag="IYA83_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ8587334.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9175..10293
                     /locus_tag="IYA83_001908"
     CDS             9175..10293
                     /locus_tag="IYA83_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ8587335.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10353..11645)
                     /locus_tag="IYA83_001909"
     CDS             complement(10353..11645)
                     /locus_tag="IYA83_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ8587336.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11795..12355
                     /locus_tag="IYA83_001910"
     CDS             11795..12355
                     /locus_tag="IYA83_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8587337.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12484..12861
                     /locus_tag="IYA83_001911"
     CDS             12484..12861
                     /locus_tag="IYA83_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ8587338.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12794..14866
                     /locus_tag="IYA83_001912"
     CDS             12794..14866
                     /locus_tag="IYA83_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ8587339.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(15077..16024)
                     /locus_tag="IYA83_001913"
     CDS             complement(15077..16024)
                     /locus_tag="IYA83_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ8587340.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            16270..16734
                     /locus_tag="IYA83_001914"
     CDS             16270..16734
                     /locus_tag="IYA83_001914"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ8587341.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVFPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(16796..17839)
                     /locus_tag="IYA83_001915"
     CDS             complement(16796..17839)
                     /locus_tag="IYA83_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ8587342.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5880 a         3257 c         3815 g         5011 t
ORIGIN      
        1 tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat
       61 tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac
      121 atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat
      181 ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata
      241 ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat
      301 tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg
      361 gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc
      421 ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc
      481 gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt
      541 ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat
      601 ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag
      661 tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa
      721 caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg
      781 ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat
      841 cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt
      901 aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct
      961 gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt
     1021 gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt
     1081 caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca
     1141 atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg
     1201 caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc
     1261 gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga
     1321 ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt
     1381 caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag
     1441 cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat
     1501 gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg
     1561 gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa
     1621 aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa
     1681 agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa
     1741 gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg
     1801 gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa
     1861 ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag
     1921 ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca
     1981 aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt
     2041 tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc
     2101 atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa
     2161 gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg
     2221 cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg
     2281 ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca
     2341 ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt
     2401 ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa
     2461 aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa
     2521 atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata
     2581 cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt
     2641 tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac
     2701 gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg
     2761 caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac
     2821 ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata
     2881 cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc
     2941 atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc
     3001 ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac
     3061 acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca
     3121 gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg
     3181 ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt
     3241 cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa
     3301 agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg
     3361 ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat
     3421 ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg
     3481 caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa
     3541 atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag
     3601 aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg tgggttattc
     3661 gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt
     3721 agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac
     3781 acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt
     3841 gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt
     3901 gttgtgcaaa cactatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag
     3961 tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg
     4021 tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc
     4081 ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta
     4141 agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg
     4201 gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc
     4261 ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa
     4321 atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg
     4381 aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg
     4441 gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt
     4501 tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa
     4561 gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa
     4621 gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc
     4681 agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca
     4741 gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga
     4801 acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc
     4861 ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat
     4921 gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag
     4981 agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg
     5041 gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa
     5101 atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca
     5161 gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa
     5221 aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat
     5281 cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt
     5341 tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca
     5401 tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag
     5461 agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa
     5521 tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa
     5581 tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa
     5641 aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg
     5701 cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc
     5761 aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa
     5821 acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca
     5881 tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa
     5941 aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta
     6001 aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac
     6061 gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt
     6121 attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg
     6181 ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta
     6241 gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa
     6301 tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat
     6361 gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt
     6421 gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata
     6481 ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat
     6541 aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa
     6601 aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca
     6661 cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca
     6721 atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa
     6781 aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac ttacttcctt
     6841 agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa
     6901 tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca
     6961 acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca
     7021 gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg
     7081 atatattata cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa
     7141 gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg
     7201 ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg
     7261 aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac
     7321 gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct
     7381 gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt
     7441 ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata
     7501 ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata
     7561 gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa
     7621 aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag agcagttgca
     7681 aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa
     7741 tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt
     7801 caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc
     7861 cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga
     7921 atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt
     7981 catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg
     8041 gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca
     8101 aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt
     8161 tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta
     8221 aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat
     8281 tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag
     8341 gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga
     8401 tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat
     8461 cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg
     8521 gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa
     8581 aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca
     8641 ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag
     8701 acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt
     8761 tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg
     8821 cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg
     8881 ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg
     8941 ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt
     9001 tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca
     9061 aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc
     9121 tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa
     9181 atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat
     9241 attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt agatgggact
     9301 agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa
     9361 ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac
     9421 atcttacaaa cgaatggagc gactggtgct aatcttttag ctttatacgc gttagtggaa
     9481 gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa
     9541 tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat
     9601 ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca
     9661 aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct
     9721 cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt
     9781 gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa
     9841 acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa
     9901 gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt
     9961 gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca
    10021 gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct
    10081 cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt
    10141 tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta
    10201 ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc
    10261 ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg
    10321 acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct
    10381 gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt
    10441 gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg
    10501 ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca
    10561 gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat
    10621 tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa
    10681 cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc
    10741 gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca
    10801 aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc
    10861 aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta
    10921 atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt
    10981 ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg
    11041 acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa gatgatccct
    11101 gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt
    11161 ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt
    11221 tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc
    11281 ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa
    11341 atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg
    11401 gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc
    11461 aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt
    11521 gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag
    11581 acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca
    11641 gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa
    11701 tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga
    11761 gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga
    11821 gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt
    11881 tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa
    11941 tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct
    12001 gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta
    12061 tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac
    12121 caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt
    12181 tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat
    12241 gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga
    12301 tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat
    12361 cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt
    12421 cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg
    12481 agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac
    12541 tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg
    12601 tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac
    12661 tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat
    12721 ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc
    12781 agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct
    12841 aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat
    12901 cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga
    12961 atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc
    13021 acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt
    13081 ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat
    13141 cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga
    13201 taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta
    13261 tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc
    13321 cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc
    13381 attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat
    13441 cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa
    13501 aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac
    13561 agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat
    13621 ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca
    13681 aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac
    13741 aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga
    13801 ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag
    13861 taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt
    13921 aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc
    13981 tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat
    14041 ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga
    14101 aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact
    14161 atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag
    14221 tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat
    14281 ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt
    14341 aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat
    14401 tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg
    14461 cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa
    14521 gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta
    14581 tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca
    14641 tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt
    14701 ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa
    14761 aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga
    14821 agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga
    14881 ggttgtgata gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
    14941 aaatttttgt gaaatttcgg cttatttccg aagaagctac ttctggaaca ccatttattc
    15001 ggaaaaaagg actgtgacaa gacttttgtc acagtccttt ttcatttaaa aaaatgttta
    15061 cttcatttga cgtatattat tggtgaggct tcaattcatt tggcttcact ttttctgttt
    15121 ctcttatgat ataaacagga cgctgtttcg tttctaagaa gattttccca atatatttcc
    15181 cgatgattcc aagacataac aattggattc caccaatgaa caggaagatc gagaccatgg
    15241 aaggccatcc tgatgtagga tcaccaaaca acaatgcccg caaaataatg aacaaaattg
    15301 caataccaga accgacacaa gacaaagcgc cgataaatga agcaatcgtt aacggtgcat
    15361 ccgagaagtt cacgatacca tcaatcgagt agttgaataa tttccagaag gaccaagatg
    15421 tttcgccagc cacacgttcg cggttttcat aagaaatata ttctgtttta aatccgaccc
    15481 agctaaataa tccttttgaa aatcggttgt attctgttaa ttctaggacg gcatccacca
    15541 tttgacgagt catcaagcgg aaatcacgag cgccatcgac catttctgta tcgctgattt
    15601 tattgatcaa ctggtagaat tttttcgcaa agaaactgcg tatcgcaggc tcgccttcac
    15661 gagtcgaacg ccgggtgccg acacaatcga tatcagaagt agtgatccgg ttgatcatct
    15721 caggaagcag ttcgggcgga tcttgcagat caacatccat cacggtcact aaatctcctg
    15781 tagaagcttg caatccagcg tacagagccg cttctttccc aaaatttctg gaaaatgaga
    15841 caaagcggac ataaggataa gctgaggcta gcttcttgat tgctgctaac gtaccgtctt
    15901 ttgatccgtc gtcaataaac aaatattcga actcatgggc acctttgaat ttttcaacct
    15961 catcaaaaaa aagaggaaca gttttttctt cattaaaaca tgggacaaca attgatactt
    16021 tcatcgtttc tatcctttca atcgtatgtt tatatatggt ttacattcaa aatttaataa
    16081 accttaaata acacctttaa aatttgattt tacgtaaaag aacaggtatt ctttttgtaa
    16141 actaacgtat ttagtttacc atgtttcctc ggtttatcaa acttttaaga acaattttta
    16201 ttagttggca agtagttttg gatagagaaa aaggaggaag ttccagtcta ttgactagga
    16261 gcaaacaaaa tgaaaataca cacacctatc ggctttttat ggttagatgc agatgaatcc
    16321 ggattgactg ctgtatcttt tcagccaatt tcagaaagcg aatcagcgaa tcagcgaatt
    16381 ttaacgcgca cacaagaaca gtttgaagaa tattttgcgg gaaaaagaaa agaatttgat
    16441 gttcctttat ctataaagag aggtacgcct tttcagcaaa atgtctggaa agctttgtcg
    16501 accattcctt atggagaaat ccgaacgtat aaagaagtag ccgttgctgt tgatagtccg
    16561 aaagctgtgc gagcgattgg gcaggcaaat cgaaccaatc cattgccaat cgtttttcct
    16621 tgtcatcgag tgatcgggca aaatggaaaa ctgacaggat atatgggaaa cgcaatagat
    16681 ggggttgaaa tcaaacgcca attgttgttg ttagaaggat atctgcaaaa ttaaaaacag
    16741 ttgtgattcg gacattgttc catgcgagaa tcacaactgt tttttcatgt tcattctaaa
    16801 ctttgatcag ccaaataaat agtaaagcca tcactgtttg gagaagcccg acagctaatc
    16861 cataagataa gaagcgtgta ccttctttca tgaattttgc aaagtctaaa cgcagaccaa
    16921 ttgccgctaa agcagtgatc tcaaaccagc tgctgataaa atgtgccgat tggtcaataa
    16981 ctacaggtaa agaaaacaag ctgttgacca aacaaaagat cacaaaccct aatacatacc
    17041 aaggaagcgg taattttcgt tttttctgcg tatcagttgg cgtttcaaac agagctggtt
    17101 gtttcttgtg catgaatttt tcaaacagat agacaacaac aacgagcatc ataatacgta
    17161 gaatcttaaa tagcatggcg aattcgacaa cagaattgtc aagtaagctt gccccagcga
    17221 ctacttgacc aacagattgg agtgtaccac ccaaaagtgc acttttttca atgagatttc
    17281 caccaaacaa ctgtaatcca agaaaaggga gagtcaacat catcacagta cccagtagat
    17341 tgactagtgt gatgatctgt cctttttctt cctctttcgc atggatggaa ggggcgattg
    17401 aagcaatagc agaagaacca caaacagcgt ttcctccagc catcatcaaa gaagaagtat
    17461 cgctgaattg cagtttttta ccaatcaagt aagcaccgat gatagtgact gacatttgca
    17521 gtaacacaaa aacaattcct tttacaccaa tttgagcaat cgtttgagct gtgacagttg
    17581 cacctagtaa aaaaacagag aattctaata gtttgctttc agcaaatttt gtgccgattg
    17641 ctagaacagg ctgtttaaaa aaagtatttc ctaataaaat acccaataaa atagcaatag
    17701 tcgctccacc taactgtggc agataaatag caagaagcct gctgaaggca gcgatggcaa
    17761 aagaaagcag taaaccgggt aagatttcgc ggaccgctga atcctttaca gattttattg
    17821 gatgtgacaa tgatttcatt tgatcggttc ctttcgtcaa taaatactaa aagcatactc
    17881 gaacatagta gcactaaacc agtattttga caaaagaaca agaggtcgtg acaaaagtct
    17941 tgtacgacct cttgtttcga ata
//
LOCUS       DACIFS010000052        17587 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.052, whole genome shotgun sequence.
ACCESSION   DACIFS010000052 DACIFS010000000
VERSION     DACIFS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.052"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            363..731
                     /locus_tag="IYA83_001916"
     CDS             363..731
                     /locus_tag="IYA83_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ8587343.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IYA83_001917"
     CDS             911..1852
                     /locus_tag="IYA83_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ8587344.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IYA83_001918"
     CDS             1921..2652
                     /locus_tag="IYA83_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ8587345.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IYA83_001919"
     CDS             2796..4976
                     /locus_tag="IYA83_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ8587346.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IYA83_001920"
     CDS             4996..5478
                     /locus_tag="IYA83_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ8587347.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IYA83_001921"
     CDS             5494..6255
                     /locus_tag="IYA83_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ8587348.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IYA83_001922"
     CDS             6386..7720
                     /locus_tag="IYA83_001922"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ8587349.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IYA83_001923"
     CDS             8027..8512
                     /locus_tag="IYA83_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ8587350.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IYA83_001924"
     CDS             8505..9188
                     /locus_tag="IYA83_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ8587351.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IYA83_001925"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IYA83_001925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ8587352.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IYA83_001926"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IYA83_001926"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ8587353.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IYA83_001927"
     CDS             12804..13433
                     /locus_tag="IYA83_001927"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ8587354.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IYA83_001928"
     CDS             13485..13817
                     /locus_tag="IYA83_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ8587355.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IYA83_001929"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IYA83_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ8587356.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IYA83_001930"
     CDS             14643..16016
                     /locus_tag="IYA83_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ8587357.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IYA83_001931"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IYA83_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ8587358.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IYA83_001932"
     CDS             17071..17511
                     /locus_tag="IYA83_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ8587359.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5992 a         3067 c         3866 g         4662 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatggga
//
LOCUS       DACIFS010000053        17018 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.053, whole genome shotgun sequence.
ACCESSION   DACIFS010000053 DACIFS010000000
VERSION     DACIFS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.053"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..2435)
                     /gene="parC"
                     /locus_tag="IYA83_001933"
     CDS             complement(<1..2435)
                     /gene="parC"
                     /locus_tag="IYA83_001933"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ8587360.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPE"
     gene            complement(2447..4492)
                     /gene="parE"
                     /locus_tag="IYA83_001934"
     CDS             complement(2447..4492)
                     /gene="parE"
                     /locus_tag="IYA83_001934"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ8587361.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4658..5092)
                     /locus_tag="IYA83_001935"
     CDS             complement(4658..5092)
                     /locus_tag="IYA83_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ8587362.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5219..5857
                     /gene="plsY"
                     /locus_tag="IYA83_001936"
     CDS             5219..5857
                     /gene="plsY"
                     /locus_tag="IYA83_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ8587363.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(5989..6864)
                     /locus_tag="IYA83_001937"
     CDS             complement(5989..6864)
                     /locus_tag="IYA83_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ8587364.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(6946..7737)
                     /gene="codY"
                     /locus_tag="IYA83_001938"
     CDS             complement(6946..7737)
                     /gene="codY"
                     /locus_tag="IYA83_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ8587365.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="IYA83_001939"
     CDS             complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="IYA83_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ8587366.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="IYA83_001940"
     CDS             complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="IYA83_001940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ8587367.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="IYA83_001941"
     CDS             complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="IYA83_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ8587368.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="IYA83_001942"
     CDS             complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="IYA83_001942"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ8587369.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12202..14280)
                     /gene="topA"
                     /locus_tag="IYA83_001943"
     CDS             complement(12202..14280)
                     /gene="topA"
                     /locus_tag="IYA83_001943"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ8587370.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="IYA83_001944"
     CDS             complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="IYA83_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ8587371.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15315..16082)
                     /locus_tag="IYA83_001945"
     CDS             complement(15315..16082)
                     /locus_tag="IYA83_001945"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ8587372.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="IYA83_001946"
     CDS             complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="IYA83_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ8587373.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4330 a         3801 c         2959 g         5928 t
ORIGIN      
        1 atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc catcgcaaat
       61 gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt taactgctgc
      121 cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata gataatacgg
      181 tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg tgaagtttgg
      241 ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat tacgatcggc
      301 gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc tttcaaattc
      361 atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa aggatagcgc
      421 aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc ttctttcaga
      481 tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag cggacggctc
      541 ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa tccattcttg
      601 ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac agctaaaatc
      661 gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc atctttccat
      721 ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt tgtgatcaga
      781 agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc ttctcgcatc
      841 ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt cacatagcct
      901 tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt atcaattttg
      961 atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt ttttttcact
     1021 tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag gattttattc
     1081 aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt gatatccgtg
     1141 ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc tgtaaattga
     1201 aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg aatcgttgcg
     1261 attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg ctgtcttttt
     1321 tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg atgctcgata
     1381 taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc gatagcgacc
     1441 atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt caaaattcct
     1501 tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct atcactttca
     1561 tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc catttttttc
     1621 accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg ctgtttatta
     1681 cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg tcctgtttca
     1741 taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa gtcaggtcca
     1801 ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat atagattgtg
     1861 ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc agcagatata
     1921 cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt tggttctttt
     1981 tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc tgctagcatc
     2041 tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg tggatcaccg
     2101 tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt ccagtcttgg
     2161 ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata attccccatg
     2221 atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt gccatctttg
     2281 ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat atcaggcagt
     2341 gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat cacttcttct
     2401 aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat tctacttcaa
     2461 ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca ttagaaactg
     2521 aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct tcgagcgtaa
     2581 attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc atcagtgtgg
     2641 ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac gtacgggaag
     2701 tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt cctttatatc
     2761 gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg tcatctgtcc
     2821 acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat aaaggcggta
     2881 acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa aagaaagtaa
     2941 gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg attactttgt
     3001 cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc gtatagatca
     3061 tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg gtattgatga
     3121 cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt ttagcagatc
     3181 cgccagcaga atccccttcg actaaataca attcattttt cttagggttt cgggattgtg
     3241 cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc ccatttcggc
     3301 tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt gctttacgga
     3361 ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt tcactgatga
     3421 cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt tgcccttcaa
     3481 attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct tctctgaaat
     3541 cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc gcatattcat
     3601 tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca tcttttgtcc
     3661 gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat tgataagcga
     3721 cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc aatgtatctt
     3781 tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga aaaatttcct
     3841 tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt agaagaaagg
     3901 cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta ctgaatattg
     3961 tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt cctatctttt
     4021 tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct acgccatctc
     4081 taacgatcgt tacaattagc catttcgata aggcatttac cacactggct cctacaccgt
     4141 gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca tgcagcacag
     4201 taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc atcccacgtc
     4261 cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc tcactgccat
     4321 aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa tgatgcaagc
     4381 ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc tctaatcctt
     4441 ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc aaggcaaacg
     4501 ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag aaaaggtcgt
     4561 gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc tttttcgaca
     4621 ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt tctttcaact
     4681 taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct gcttttctta
     4741 gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact ttagcatcag
     4801 ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat acatctacga
     4861 tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct agaatttctt
     4921 caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc aatacctgtg
     4981 caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca atattttttg
     5041 cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac atttctgcca
     5101 ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt ttcaattgtt
     5161 cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag ggtaaaaaat
     5221 gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt ccggtgtctg
     5281 gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta atactggaac
     5341 gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc ttatggatat
     5401 tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg ggattaaccc
     5461 tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg ctgggttcaa
     5521 aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc ctgtattttt
     5581 tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg tgagtttgag
     5641 cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt atgtagctcc
     5701 tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag tttcttcctt
     5761 tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg aaagtcgaat
     5821 cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc caaagagata
     5881 aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat agctttttta
     5941 cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt acttcactgt
     6001 gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc ctactttttc
     6061 catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt cgatacaaac
     6121 gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat ctttatagct
     6181 taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta gaccccttgt
     6241 ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat tcgtatttgt
     6301 ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta ggcgtaatct
     6361 tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt cttcaagagg
     6421 tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac cggtatttgc
     6481 cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca ctaattcaaa
     6541 atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac tagccatttc
     6601 ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt gccccatttg
     6661 atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa aagggaataa
     6721 aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact ctaaaccatg
     6781 ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg cgatcagctg
     6841 gtcattttga ataataacag tcattgccat tcctccaact tcgacttact ttttaaaatg
     6901 ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat aactgtgttt
     6961 tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct tcatgcctaa
     7021 cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga cgatcacaga
     7081 acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt catctccatc
     7141 caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg tgttgattgc
     7201 catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact tctgataaag
     7261 gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt catctgcaaa
     7321 cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc caaagatcgg
     7381 aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa atgcagtaat
     7441 atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg tatcagtata
     7501 agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt tcacatcgtg
     7561 acgttcatta taacctagta atactccgtc acttcctatg atataggtgt tgctgtcaag
     7621 aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca tgtcaaaagt
     7681 attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt ctgtcattgt
     7741 cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc aattgagcca
     7801 agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat atctggtgct
     7861 tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg tgcgccgata
     7921 ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat tgcttctttt
     7981 gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt tactaaagca
     8041 ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc taattctacc
     8101 cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga caaatggaaa
     8161 gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt tgtgtttact
     8221 tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga tacttctcca
     8281 gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat tccactttct
     8341 tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt tgctgattct
     8401 tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac ttttttcttc
     8461 ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa tccattgttc
     8521 atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg actatccagt
     8581 aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc ttctgtcact
     8641 tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat catttgttca
     8701 aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat ttttaccaaa
     8761 cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc tttttctacg
     8821 atttggatcg cattttctac taaatcccga accattgatt ctacatctct tccgacataa
     8881 ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac gatttttgcc
     8941 aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag catatttttt
     9001 ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg gttacgcaaa
     9061 gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc taattcactt
     9121 acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt acagttcttc
     9181 tacgataata ttatgattag taaagataca gatatctgcg gcaatattta acgcattttc
     9241 tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc gggctgcagc
     9301 caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg tgataacttc
     9361 tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca tcgcttcaag
     9421 tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg cgcgttgtag
     9481 attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa acgcatctgc
     9541 aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta ctttctttgc
     9601 agtacctttc atgacaacag attcacccat tgttacttgg ccatctccag ccatagcaaa
     9661 tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt cgaccatgtt
     9721 tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga aagaacgata
     9781 gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg ataaattggc
     9841 atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata aatgagtagc
     9901 aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt ttttgatcat
     9961 ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat gattcacaaa
    10021 aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt ccctgaaata
    10081 ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt tgttcccttt
    10141 accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca aactgctaca
    10201 ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt tcctcaaatc
    10261 caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt cataaaaaaa
    10321 acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat tttcttttat
    10381 cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct tgcgactgat
    10441 agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga cacgaaccgt
    10501 aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat gtttgaaatg
    10561 gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt cgacaatcaa
    10621 ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt ccttttgtcc
    10681 cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga atttaaccaa
    10741 tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag aaattcttaa
    10801 gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga gtttccttac
    10861 tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc ttttaaagct
    10921 cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg cagttcagga
    10981 aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg agtaatgtag
    11041 taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat ttcttttgct
    11101 aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac atagccttct
    11161 acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg gtaagtcggt
    11221 ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata gcgaacgaat
    11281 tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc ccatttcaag
    11341 tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg acgcagctgg
    11401 ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg tttcataggt
    11461 ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat acatccttca
    11521 aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat tagtgcctca
    11581 tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc ttctcctttg
    11641 tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc tacgattggt
    11701 gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat gctttctgct
    11761 aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg tatttcagta
    11821 atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt aattgtttca
    11881 gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt ttcatctgcc
    11941 gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc tactgcatta
    12001 gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt acttgtgtga
    12061 tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac tcctgcttgt
    12121 gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt taccattttc
    12181 aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc catttacaca
    12241 gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac attttggaca
    12301 atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac gggaacaacc
    12361 atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat gacatactgg
    12421 gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag gaaaattact
    12481 gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc cacagacatc
    12541 acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt cttccgcatt
    12601 cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc acgcttccgt
    12661 gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga tatcgacgat
    12721 ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag tcggctcaaa
    12781 tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg ttggtgcata
    12841 agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag cctcactaaa
    12901 acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt ggactacatc
    12961 gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat ctctaccttc
    13021 cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa tgaagatgac
    13081 accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca tctgactagc
    13141 cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta aatattgttt
    13201 gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg catcttgtgc
    13261 gccttgagca tttttgatct ttcggtgacc atgtctagaa aattcttttc catacgtatc
    13321 ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag aatcagtacg
    13381 catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt catataattg
    13441 ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag cttcttgttg
    13501 caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct tttctacttt
    13561 tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac tttgtgcatc
    13621 tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt tcttgcgagc
    13681 ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc ggatctcttt
    13741 ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac ttaacccttt
    13801 cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt ctaaggtacg
    13861 acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct taaaagctgc
    13921 tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt tcagatccaa
    13981 cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg ggtcacttgc
    14041 caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga catcgccttt
    14101 tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac ccattttact
    14161 tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc gtccaagata
    14221 tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt tatacgccat
    14281 tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat tcatacaaat
    14341 cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga aaagtatggt
    14401 gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact gcaccgtctt
    14461 ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga acagaaaaaa
    14521 ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt tttttcttgg
    14581 cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc attggaaaac
    14641 aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga tcatttgcaa
    14701 tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct gttccaatga
    14761 ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta tctatccctt
    14821 tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt aaataataaa
    14881 ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac atgttacaag
    14941 aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc aagtgaagca
    15001 gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat tggattttgc
    15061 cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt ttggttattt
    15121 tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc atcatccagt
    15181 tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt ttcaataaat
    15241 tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat aagatacgta
    15301 aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt acgatggatt
    15361 ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata acctgcatta
    15421 tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg gtctcgatat
    15481 actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc tttgattatt
    15541 ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata gcttggtaca
    15601 atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata aatattcaca
    15661 tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt ttcttgaact
    15721 tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt cagccctaat
    15781 atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc taatggtcct
    15841 cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc ttgcttttca
    15901 aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa tcgtcgttca
    15961 aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga ttctagtgta
    16021 ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat ggattcagtc
    16081 atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa gttttccttg
    16141 acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat acccacgttt
    16201 tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga ttgataaacc
    16261 ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc cgtaaatcgc
    16321 taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta gttttttgcc
    16381 gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca attccaactc
    16441 attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc ctgtctgtgc
    16501 aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg gaatacctaa
    16561 gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg cccgtttttc
    16621 tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt cttgtgccgt
    16681 gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct ttgtctgttg
    16741 tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct gaatgatctg
    16801 atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt caaatacgat
    16861 gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat gtcccggaaa
    16921 ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc cggctttact
    16981 tgtttttttc tttttcaata taaattcact tttttatt
//
LOCUS       DACIFS010000054        16941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.054, whole genome shotgun sequence.
ACCESSION   DACIFS010000054 DACIFS010000000
VERSION     DACIFS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.054"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(258..1763)
                     /locus_tag="IYA83_001947"
     CDS             complement(258..1763)
                     /locus_tag="IYA83_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8587374.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="IYA83_001948"
     CDS             complement(1760..2446)
                     /locus_tag="IYA83_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8587375.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IYA83_001949"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="IYA83_001949"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ8587376.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IYA83_001950"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="IYA83_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ8587377.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="IYA83_001951"
     CDS             complement(4467..5036)
                     /locus_tag="IYA83_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ8587378.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="IYA83_001952"
     CDS             5222..6124
                     /locus_tag="IYA83_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ8587379.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IYA83_001953"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="IYA83_001953"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ8587380.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IYA83_001954"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="IYA83_001954"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ8587381.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IYA83_001955"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="IYA83_001955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ8587382.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="IYA83_001956"
     CDS             12692..12877
                     /locus_tag="IYA83_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ8587383.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="IYA83_001957"
     CDS             complement(12939..14135)
                     /locus_tag="IYA83_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ8587384.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="IYA83_001958"
     CDS             complement(14305..15741)
                     /locus_tag="IYA83_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ8587385.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="IYA83_001959"
     CDS             16034..16843
                     /locus_tag="IYA83_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587386.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4968 a         3550 c         2961 g         5462 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa tttttgggct ctataattta tgggactgat gaatcagaat
    16921 tgattcttca gccccatttt c
//
LOCUS       DACIFS010000055        16537 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.055, whole genome shotgun sequence.
ACCESSION   DACIFS010000055 DACIFS010000000
VERSION     DACIFS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.055"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYA83_001960"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IYA83_001960"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ8587387.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IYA83_001961"
     CDS             complement(1174..1869)
                     /locus_tag="IYA83_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ8587388.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IYA83_001962"
     CDS             complement(1882..2598)
                     /locus_tag="IYA83_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ8587389.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="IYA83_001963"
     CDS             2928..3533
                     /locus_tag="IYA83_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ8587390.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="IYA83_001964"
     CDS             3858..4181
                     /locus_tag="IYA83_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ8587391.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="IYA83_001965"
     CDS             4363..5733
                     /locus_tag="IYA83_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ8587392.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="IYA83_001966"
     CDS             complement(5863..7164)
                     /locus_tag="IYA83_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ8587393.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="IYA83_001967"
     CDS             complement(7394..9385)
                     /locus_tag="IYA83_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ8587394.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="IYA83_001968"
     CDS             complement(9529..9795)
                     /locus_tag="IYA83_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ8587395.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IYA83_001969"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="IYA83_001969"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ8587396.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IYA83_001970"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="IYA83_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ8587397.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /locus_tag="IYA83_001971"
     CDS             13139..13702
                     /locus_tag="IYA83_001971"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ8587398.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="IYA83_001972"
     CDS             complement(13955..14476)
                     /locus_tag="IYA83_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ8587399.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="IYA83_001973"
     CDS             complement(14479..15321)
                     /locus_tag="IYA83_001973"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ8587400.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="IYA83_001974"
     CDS             complement(15314..16039)
                     /locus_tag="IYA83_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ8587401.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="IYA83_001975"
     CDS             16215..16511
                     /locus_tag="IYA83_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587402.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4652 a         3458 c         3062 g         5365 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttataacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgat acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacagg
//
LOCUS       DACIFS010000056        16247 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.056, whole genome shotgun sequence.
ACCESSION   DACIFS010000056 DACIFS010000000
VERSION     DACIFS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.056"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYA83_001976"
     CDS             complement(21..1586)
                     /gene="guaA"
                     /locus_tag="IYA83_001976"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ8587403.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1645..2568)
                     /locus_tag="IYA83_001977"
     CDS             complement(1645..2568)
                     /locus_tag="IYA83_001977"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ8587404.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2764..3369
                     /locus_tag="IYA83_001978"
     CDS             2764..3369
                     /locus_tag="IYA83_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8587405.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3677..4693
                     /locus_tag="IYA83_001979"
     CDS             3677..4693
                     /locus_tag="IYA83_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8587406.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4834..6135
                     /locus_tag="IYA83_001980"
     CDS             4834..6135
                     /locus_tag="IYA83_001980"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ8587407.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6181..6843
                     /gene="deoC"
                     /locus_tag="IYA83_001981"
     CDS             6181..6843
                     /gene="deoC"
                     /locus_tag="IYA83_001981"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ8587408.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6864..7262
                     /locus_tag="IYA83_001982"
     CDS             6864..7262
                     /locus_tag="IYA83_001982"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ8587409.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7328..8410
                     /locus_tag="IYA83_001983"
     CDS             7328..8410
                     /locus_tag="IYA83_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8587410.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8622..10184
                     /locus_tag="IYA83_001984"
     CDS             8622..10184
                     /locus_tag="IYA83_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587411.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10177..11310
                     /locus_tag="IYA83_001985"
     CDS             10177..11310
                     /locus_tag="IYA83_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587412.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11310..12266
                     /locus_tag="IYA83_001986"
     CDS             11310..12266
                     /locus_tag="IYA83_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587413.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12365..13531
                     /gene="deoB"
                     /locus_tag="IYA83_001987"
     CDS             12365..13531
                     /gene="deoB"
                     /locus_tag="IYA83_001987"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ8587414.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13555..14373
                     /locus_tag="IYA83_001988"
     CDS             13555..14373
                     /locus_tag="IYA83_001988"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ8587415.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14390..15097
                     /gene="deoD"
                     /locus_tag="IYA83_001989"
     CDS             14390..15097
                     /gene="deoD"
                     /locus_tag="IYA83_001989"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ8587416.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="IYA83_001990"
     CDS             complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="IYA83_001990"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ8587417.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT         5043 a         3004 c         3618 g         4582 t
ORIGIN      
        1 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
       61 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
      121 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
      181 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
      241 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
      301 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
      361 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
      421 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
      481 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
      541 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
      601 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
      661 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
      721 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
      781 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
      841 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
      901 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
      961 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
     1021 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
     1081 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
     1141 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
     1201 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
     1261 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
     1321 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
     1381 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
     1441 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
     1501 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
     1561 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
     1621 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
     1681 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
     1741 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
     1801 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
     1861 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
     1921 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
     1981 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
     2041 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
     2101 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
     2161 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
     2221 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
     2281 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
     2341 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
     2401 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
     2461 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
     2521 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
     2581 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
     2641 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
     2701 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
     2761 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
     2821 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
     2881 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
     2941 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
     3001 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
     3061 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
     3121 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
     3181 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
     3241 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
     3301 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
     3361 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
     3421 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
     3481 ttcaaaaaga aataatagga acagagtcgt tgttttttac gataaaataa ataagataca
     3541 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
     3601 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
     3661 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
     3721 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
     3781 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
     3841 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
     3901 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
     3961 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
     4021 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
     4081 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
     4141 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
     4201 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
     4261 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
     4321 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
     4381 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
     4441 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
     4501 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
     4561 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
     4621 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
     4681 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
     4741 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
     4801 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
     4861 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
     4921 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
     4981 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
     5041 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
     5101 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
     5161 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
     5221 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
     5281 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
     5341 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
     5401 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
     5461 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
     5521 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
     5581 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
     5641 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
     5701 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
     5761 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
     5821 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
     5881 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
     5941 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
     6001 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
     6061 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
     6121 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
     6181 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
     6241 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
     6301 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
     6361 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
     6421 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
     6481 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
     6541 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
     6601 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
     6661 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
     6721 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
     6781 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
     6841 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
     6901 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
     6961 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
     7021 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
     7081 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
     7141 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
     7201 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
     7261 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
     7321 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
     7381 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
     7441 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
     7501 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
     7561 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
     7621 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
     7681 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
     7741 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
     7801 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
     7861 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
     7921 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
     7981 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
     8041 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
     8101 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
     8161 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
     8221 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
     8281 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
     8341 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
     8401 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
     8461 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
     8521 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
     8581 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
     8641 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
     8701 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
     8761 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
     8821 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
     8881 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
     8941 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
     9001 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
     9061 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
     9121 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
     9181 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
     9241 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
     9301 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
     9361 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
     9421 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
     9481 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
     9541 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
     9601 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
     9661 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
     9721 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
     9781 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
     9841 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
     9901 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
     9961 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    10021 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    10081 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    10141 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    10201 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    10261 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    10321 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    10381 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    10441 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    10501 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    10561 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    10621 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    10681 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    10741 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    10801 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    10861 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    10921 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    10981 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    11041 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    11101 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    11161 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    11221 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    11281 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    11341 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    11401 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    11461 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    11521 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    11581 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    11641 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    11701 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    11761 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    11821 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    11881 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    11941 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    12001 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    12061 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    12121 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    12181 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    12241 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    12301 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    12361 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    12421 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    12481 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    12541 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    12601 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    12661 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    12721 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    12781 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    12841 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    12901 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    12961 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    13021 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    13081 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    13141 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    13201 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    13261 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    13321 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    13381 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    13441 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    13501 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    13561 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    13621 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    13681 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    13741 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    13801 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    13861 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    13921 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    13981 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    14041 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    14101 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    14161 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    14221 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    14281 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    14341 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    14401 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    14461 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    14521 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    14581 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    14641 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    14701 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    14761 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    14821 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    14881 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    14941 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    15001 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    15061 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    15121 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    15181 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    15241 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    15301 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    15361 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    15421 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    15481 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    15541 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    15601 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    15661 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    15721 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    15781 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    15841 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    15901 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    15961 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    16021 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    16081 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    16141 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    16201 tagagaatat aaaaaaacga acagtgccca cacaactgtt cgttttt
//
LOCUS       DACIFS010000057        16158 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.057, whole genome shotgun sequence.
ACCESSION   DACIFS010000057 DACIFS010000000
VERSION     DACIFS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.057"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(285..2012)
                     /locus_tag="IYA83_001991"
     CDS             complement(285..2012)
                     /locus_tag="IYA83_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8587418.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2005..3198)
                     /locus_tag="IYA83_001992"
     CDS             complement(2005..3198)
                     /locus_tag="IYA83_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ8587419.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3385..4029)
                     /locus_tag="IYA83_001993"
     CDS             complement(3385..4029)
                     /locus_tag="IYA83_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8587420.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4123..4257)
                     /locus_tag="IYA83_001994"
     CDS             complement(4123..4257)
                     /locus_tag="IYA83_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ8587421.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4259..6391)
                     /gene="feoB"
                     /locus_tag="IYA83_001995"
     CDS             complement(4259..6391)
                     /gene="feoB"
                     /locus_tag="IYA83_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ8587422.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6388..6861)
                     /locus_tag="IYA83_001996"
     CDS             complement(6388..6861)
                     /locus_tag="IYA83_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ8587423.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7262..7486
                     /gene="nrdH"
                     /locus_tag="IYA83_001997"
     CDS             7262..7486
                     /gene="nrdH"
                     /locus_tag="IYA83_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ8587424.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7645..9804
                     /gene="nrdE"
                     /locus_tag="IYA83_001998"
     CDS             7645..9804
                     /gene="nrdE"
                     /locus_tag="IYA83_001998"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ8587425.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9854..10819
                     /gene="nrdF"
                     /locus_tag="IYA83_001999"
     CDS             9854..10819
                     /gene="nrdF"
                     /locus_tag="IYA83_001999"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ8587426.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10908..12047)
                     /locus_tag="IYA83_002000"
     CDS             complement(10908..12047)
                     /locus_tag="IYA83_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ8587427.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12356..13069)
                     /locus_tag="IYA83_002001"
     CDS             complement(12356..13069)
                     /locus_tag="IYA83_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ8587428.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13323..14024)
                     /gene="nagB"
                     /locus_tag="IYA83_002002"
     CDS             complement(13323..14024)
                     /gene="nagB"
                     /locus_tag="IYA83_002002"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ8587429.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14258..15790
                     /locus_tag="IYA83_002003"
     CDS             14258..15790
                     /locus_tag="IYA83_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ8587430.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15981..>16158)
                     /locus_tag="IYA83_002004"
     CDS             complement(15981..>16158)
                     /locus_tag="IYA83_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8587431.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5002 a         3340 c         2828 g         4988 t
ORIGIN      
        1 ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga gaatgcactt
       61 cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc tctttgactc
      121 gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga tgagctacgc
      181 attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca aatttggata
      241 tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt tttccagctt
      301 tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg atcttttcca
      361 ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa attgtctaat
      421 actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt atggatctgc
      481 tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata tttggcaaac
      541 atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat cgcttgacct
      601 agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt caagttgtca
      661 tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc gattcctatg
      721 attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat gtaggctggc
      781 actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac ttgatcttta
      841 tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat ggcatctgac
      901 tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat cgttacatca
      961 tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc tgtatcccat
     1021 tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact ccctatacca
     1081 atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg ttttttactt
     1141 ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg ctgtgtaaaa
     1201 cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc attcccgact
     1261 tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc gatcatagag
     1321 gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc catgatcgta
     1381 cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct cccgaatgaa
     1441 tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt aaaacgaacg
     1501 gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc gataccgaaa
     1561 aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa gtcaaggatc
     1621 ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa tggtacccca
     1681 atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat caaatcattc
     1741 catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt aagtcctgga
     1801 cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa aggagctgga
     1861 atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt ttgtaaaaat
     1921 gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac ccagtttgcc
     1981 actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg tttctgctaa
     2041 tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct ctacaactgc
     2101 aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca tgtctttccc
     2161 aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct tcctagcttc
     2221 taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa cagccatcag
     2281 catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat cagattcgat
     2341 attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg aacgatcgac
     2401 ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt cttcacctgt
     2461 tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa tccgtaaagc
     2521 ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt gcaggtccag
     2581 taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg cttcgtccat
     2641 cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt gttgcatccc
     2701 accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat tttctaatgc
     2761 tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta acccgtattc
     2821 tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa ccatattgat
     2881 tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat cttctccatc
     2941 aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga tcaatgtgga
     3001 tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta cgtcaaaatt
     3061 cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt tcggctggtg
     3121 atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg ttaaatgttt
     3181 tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt tctttctgaa
     3241 aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga aagaaatttg
     3301 cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt agccaagtta
     3361 atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt aaaaaaagtt
     3421 tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc ctgccgtaaa
     3481 tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg cgataactgt
     3541 ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat gcattgcttt
     3601 ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc gcttcgtttg
     3661 attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt gcttctccac
     3721 acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat attgcaatag
     3781 gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc gtacttccat
     3841 gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag aagcattgta
     3901 cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc ggttttcaac
     3961 gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga tttttttctt
     4021 tccagacatt ttctttcctc tttctttttt tactttccct catcttacta tgctctagca
     4081 aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct tgatctgttc
     4141 cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt ttaaacgagt
     4201 atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg tcgccatcct
     4261 attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt ggctgacgga
     4321 acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct aacgctacct
     4381 gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa taagctaaac
     4441 cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc cgatggatag
     4501 ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat gaataagctg
     4561 ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt tcggatactt
     4621 ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg accaatcctg
     4681 taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa acaactgcca
     4741 ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga gcataaaaat
     4801 tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt cctgcttttt
     4861 taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat tgaggaaaat
     4921 gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa cgtgtttttt
     4981 tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca gaaggagcta
     5041 cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc aactttgcag
     5101 aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg ttttcaatcg
     5161 tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata ggaatgaagg
     5221 attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg atcctcgcca
     5281 tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat tgcggtagaa
     5341 atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc tgcatccatc
     5401 ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat gtaccaaaca
     5461 atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc agataataga
     5521 tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt gcaatctgat
     5581 cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag agtgtacata
     5641 gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg tcgtccaata
     5701 gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact gctatctcgt
     5761 aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag cgtttcttgc
     5821 cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa cgtggatcat
     5881 atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc ttttcgatta
     5941 gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca ccaagaccat
     6001 aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc atcatattca
     6061 gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa agatttcgtt
     6121 ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga accaaataat
     6181 ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg ggtaggtcct
     6241 gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact gttacgcccg
     6301 gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta gttttcccgc
     6361 tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta tcaagattag
     6421 ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt cgattggatc
     6481 tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc ccatatccat
     6541 taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag tctctcctgc
     6601 ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat tgacaaagat
     6661 ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta aaatggcatt
     6721 gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga tattttgcaa
     6781 atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga aattctgctt
     6841 tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat gtattaaaat
     6901 cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa tcattctcac
     6961 atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg agcgaatctt
     7021 tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca caattttttc
     7081 tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt tcaaaaacac
     7141 aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt ttgacttttt
     7201 tactaagagt tttatactat ctattataga taataagaat gtgttagagg aggcactcac
     7261 catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg tcaaacggtt
     7321 tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc ctgaagcagt
     7381 agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg atgcagaaac
     7441 agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg actaaaagaa
     7501 tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg tcgcaaactt
     7561 ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc atcatcaaat
     7621 acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt cagttatttc
     7681 aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa taaggataaa
     7741 gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac ttttccatct
     7801 attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga attcattcaa
     7861 ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca acatttttct
     7921 ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa aaccaacgac
     7981 ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct ttactttgcg
     8041 aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca acgatatcag
     8101 ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt ggtgtcttgt
     8161 ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa ctctgctttg
     8221 caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg tgaagccggc
     8281 gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat gaaattattc
     8341 gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg cgtggtttat
     8401 ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga aaatgcagat
     8461 gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa attttatgaa
     8521 ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga aaaagaatat
     8581 ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt tgcaaatcca
     8641 aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc aaaattacaa
     8701 caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga gaatccaatc
     8761 gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca aacgccttct
     8821 gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg taacttaggc
     8881 tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt gcgtacgatg
     8941 actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc accgatccaa
     9001 aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca cacttatctt
     9061 gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa tatttacttc
     9121 atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga aagaaaacaa
     9181 gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga aaaatacgta
     9241 actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg catttttatc
     9301 ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg actgtatcat
     9361 caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga tacgagtgca
     9421 agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat cggtaaaatt
     9481 tattacccag cgccttatct atccaatgag acgattccgt attacacttc tgcttatgat
     9541 atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt cgatcaaggc
     9601 atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga atggaagaca
     9661 acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt ccacaaagga
     9721 atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat cggaagcaat
     9781 caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat attttaatta
     9841 aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg aaattgaaga
     9901 tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag atacacgtat
     9961 cccactttca aatgacctag atgattggcg tacattgtct caattggaaa aagatacagt
    10021 aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa gtggcatgga
    10081 tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca atattcaatt
    10141 catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga atacgaaaaa
    10201 agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa aaaaagctga
    10261 gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg ccagcgtctt
    10321 tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt taggaaacaa
    10381 caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat ctgtacatgg
    10441 aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag cagaacaaga
    10501 aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg aagaacgtta
    10561 tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat tcctacgcta
    10621 taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag atactgccaa
    10681 tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc atgacttctt
    10741 ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag acgacgatta
    10801 tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa aataaaaaga
    10861 gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta ttcacttgtt
    10921 tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct tctcaataaa
    10981 tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg tatgcaaatc
    11041 agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat gatcgtcaaa
    11101 ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc atctaattgt
    11161 tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat aaacaaaccg
    11221 acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat attcgtgatt
    11281 ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat tgccaaaaag
    11341 ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc agataatgtt
    11401 tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg aaagaagtga
    11461 gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat aaatggaacg
    11521 gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac acttgcagat
    11581 aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga tgctaattgt
    11641 tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa ccatttgctt
    11701 gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc cattaactga
    11761 ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa cgtcaataaa
    11821 aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg atcgaacata
    11881 taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat tttcaatatt
    11941 ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt gagtactagc
    12001 tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca tctcctccaa
    12061 ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag attttcattt
    12121 gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac ttaaacaaaa
    12181 acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa taaacggtgt
    12241 tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg aactattttt
    12301 atttggctgg gcttattact tgatgtagga catctatttt acaacctcag atcatttatt
    12361 gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac tcctcgatca
    12421 catcggcata agtgagccct tttggaagat caatcgtata gatatttttg tacactcggt
    12481 agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt ttctcttcaa
    12541 aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt tctaactttt
    12601 tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca atcccgataa
    12661 aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat ccagcaattg
    12721 cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg atgatcgttc
    12781 ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct tcatctgaac
    12841 gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca ctagcgatct
    12901 caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc gtacgcatac
    12961 ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat agcatagcga
    13021 tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc taaacaccct
    13081 ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt ttacctctta
    13141 cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa acggtgttca
    13201 atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta tttttccttt
    13261 gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc tactattgaa
    13321 aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc gtgattttgc
    13381 aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt gattgcatca
    13441 gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat tgaaccgatt
    13501 cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg tttgtttgca
    13561 ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga agcaccaggt
    13621 tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc gattggatgt
    13681 gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc ttttccatct
    13741 ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat gaaatgacgg
    13801 tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac agaagtcatt
    13861 tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg tgtgcttcct
    13921 gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat ttcaaatgct
    13981 ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat tgctccttta
    14041 tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat tagtatagac
    14101 caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag gtttcataag
    14161 atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca ctgtggacgc
    14221 tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga cattaaacaa
    14281 cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc aattaaatgc
    14341 tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat tgattaaaat
    14401 catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa atattcacaa
    14461 aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag aatttctaaa
    14521 aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt accaaacata
    14581 tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa cggaattaga
    14641 ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg ggttggggat
    14701 cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac gttcaaaggt
    14761 gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg aaccaaccaa
    14821 tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt ttgacggtgc
    14881 gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc atatcgcaga
    14941 catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat tgaaacaaaa
    15001 ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac aaatcgaaaa
    15061 gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg caaaaagccg
    15121 acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa gtaaagctgt
    15181 ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa ctgatttagt
    15241 catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct ttggtgaaaa
    15301 tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa cactgatcgg
    15361 tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa tcaattactt
    15421 ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt taagcagtca
    15481 atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag ggttagttaa
    15541 tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta aggtgaaact
    15601 tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga cgaaccatat
    15661 cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag gaacagtaat
    15721 catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca ttgagattga
    15781 accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa agttttgtca
    15841 caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt agctcaaaaa
    15901 acggcaaaat atgaggagct atttttattt ggctattctt attacttgat gcagaacagc
    15961 ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc tttaaaaaca
    16021 actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt tacacctaac
    16081 cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc tccaataaag
    16141 tacataaaca taaataca
//
LOCUS       DACIFS010000058        15737 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.058, whole genome shotgun sequence.
ACCESSION   DACIFS010000058 DACIFS010000000
VERSION     DACIFS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.058"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(28..312)
                     /locus_tag="IYA83_002005"
     CDS             complement(28..312)
                     /locus_tag="IYA83_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ8587432.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(302..1036)
                     /locus_tag="IYA83_002006"
     CDS             complement(302..1036)
                     /locus_tag="IYA83_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ8587433.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1165..1788
                     /locus_tag="IYA83_002007"
     CDS             1165..1788
                     /locus_tag="IYA83_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ8587434.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1980..3101
                     /locus_tag="IYA83_002008"
     CDS             1980..3101
                     /locus_tag="IYA83_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ8587435.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3108..6236
                     /locus_tag="IYA83_002009"
     CDS             3108..6236
                     /locus_tag="IYA83_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ8587436.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6255..9449
                     /locus_tag="IYA83_002010"
     CDS             6255..9449
                     /locus_tag="IYA83_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ8587437.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9595..10302)
                     /locus_tag="IYA83_002011"
     CDS             complement(9595..10302)
                     /locus_tag="IYA83_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ8587438.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            11067..11465
                     /gene="spxA"
                     /locus_tag="IYA83_002012"
     CDS             11067..11465
                     /gene="spxA"
                     /locus_tag="IYA83_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ8587439.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            11797..12450
                     /locus_tag="IYA83_002013"
     CDS             11797..12450
                     /locus_tag="IYA83_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ8587440.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            12676..13674
                     /locus_tag="IYA83_002014"
     CDS             12676..13674
                     /locus_tag="IYA83_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ8587441.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            13850..15658
                     /gene="pepF"
                     /locus_tag="IYA83_002015"
     CDS             13850..15658
                     /gene="pepF"
                     /locus_tag="IYA83_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ8587442.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         5475 a         2635 c         3243 g         4384 t
ORIGIN      
        1 tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg
       61 tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt
      121 tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt
      181 ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag
      241 atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg
      301 ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag
      361 tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta
      421 acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa
      481 ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag
      541 taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg
      601 tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca
      661 gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag
      721 ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg
      781 cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg
      841 cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa
      901 tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag
      961 aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt
     1021 cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta
     1081 tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac
     1141 cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta
     1201 ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat
     1261 gagaattaca tactagttgc tccccatcgt acttggtggg acccattata tctggcggta
     1321 gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt
     1381 ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca
     1441 agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt
     1501 cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg
     1561 tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc
     1621 aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa
     1681 acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg
     1741 gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt
     1801 atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac
     1861 gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg
     1921 tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa
     1981 tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga
     2041 ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag
     2101 aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag
     2161 aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta
     2221 tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt
     2281 cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg
     2341 tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg
     2401 acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat
     2461 ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat
     2521 ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta
     2581 caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata
     2641 atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag
     2701 gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc
     2761 tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc
     2821 agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga
     2881 atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg
     2941 gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat
     3001 tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat
     3061 ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa
     3121 aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga
     3181 cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg
     3241 atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga
     3301 tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc
     3361 agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa
     3421 acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag
     3481 aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc
     3541 tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt
     3601 tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt
     3661 tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc
     3721 atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac
     3781 cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa
     3841 aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag
     3901 aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa
     3961 taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga
     4021 ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa
     4081 aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga
     4141 ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa
     4201 aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat
     4261 ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat
     4321 tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc
     4381 aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa
     4441 aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc
     4501 tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc
     4561 tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa
     4621 ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg
     4681 aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag
     4741 aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa
     4801 gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg
     4861 caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat
     4921 ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag
     4981 atagacgaaa aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag
     5041 cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc
     5101 agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata
     5161 gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc
     5221 aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga
     5281 cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac
     5341 aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct
     5401 gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc
     5461 atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg
     5521 agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg
     5581 aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct
     5641 acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat
     5701 tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt
     5761 atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt
     5821 taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa
     5881 ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc
     5941 tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga
     6001 tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt
     6061 cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag
     6121 gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa
     6181 taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag
     6241 ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat
     6301 acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg
     6361 cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag
     6421 attactgcca atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg
     6481 aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag
     6541 aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa
     6601 cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg
     6661 agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat
     6721 tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca
     6781 cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt
     6841 ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat
     6901 tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc
     6961 cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc
     7021 agataaatga acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat
     7081 tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt
     7141 tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc
     7201 aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag
     7261 tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga
     7321 tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat
     7381 ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg
     7441 gcgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg
     7501 tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc
     7561 agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt
     7621 tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc
     7681 gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt
     7741 catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc
     7801 tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt
     7861 ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac
     7921 gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat
     7981 tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc
     8041 atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc
     8101 aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag
     8161 ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag
     8221 aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc
     8281 aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa
     8341 aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct
     8401 taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg
     8461 cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac
     8521 ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat
     8581 tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg
     8641 cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt
     8701 tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa
     8761 aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg
     8821 aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt
     8881 gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag
     8941 taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat
     9001 ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt
     9061 atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg
     9121 gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg
     9181 gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg
     9241 ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg
     9301 aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa
     9361 aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga
     9421 ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa
     9481 gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga
     9541 ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac
     9601 aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat
     9661 tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact
     9721 ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag
     9781 catatgaaat tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt
     9841 tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag
     9901 tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc
     9961 cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc
    10021 ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc
    10081 ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat
    10141 tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc
    10201 tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc
    10261 atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg
    10321 gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa
    10381 gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta
    10441 tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta
    10501 atcattatgg tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag
    10561 atttattttt ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat
    10621 ttatttgttt atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata
    10681 acagagaaaa taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac
    10741 tattaaagaa aaaaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga
    10801 taaaataata gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt
    10861 gaaaactcta atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac
    10921 cttagataac tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg
    10981 attatgtata atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt
    11041 cttatcacga aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt
    11101 cctgcagaaa ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata
    11161 ttttttcaga accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg
    11221 gaacagaaga aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag
    11281 atgatctgcc gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc
    11341 gtccaatcat gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc
    11401 ggttcctacc gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat
    11461 tatagaagga taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt
    11521 tttgttttta caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat
    11581 tgcacgaaaa atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc
    11641 aaaaatgtta tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa
    11701 attcgcttaa cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc
    11761 tatattaaac attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa
    11821 aatacgatcc gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg
    11881 gatttacttg gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt
    11941 gatatcgaag aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga
    12001 gacggattag aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa
    12061 gattctactg ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc
    12121 gatccggagg aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt
    12181 gcttttgaag attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct
    12241 tggactgatt tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa
    12301 aatctcaacc aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag
    12361 agcgatcgta caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct
    12421 atcgagcgaa cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa
    12481 aattgtgtcg cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca
    12541 agcccaacca aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat
    12601 gcagaatagc tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag
    12661 ttcagaggga gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa
    12721 catgcagaaa aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa
    12781 aagggaaaga aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt
    12841 agtgaaagag aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga
    12901 cagtcagtag aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat
    12961 attttgttgg cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt
    13021 attgaaagaa cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat
    13081 tcttttcttg gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga
    13141 aaaagaggag tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat
    13201 catattacag agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct
    13261 agtagggacc ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa
    13321 atagaaaaag tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa
    13381 aagaatacag tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta
    13441 tcatcgtgga tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt
    13501 tatcgaatgt tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg
    13561 ctttgccaaa taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg
    13621 gtctatcagc agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta
    13681 taaaaatagg ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga
    13741 tggaagcaga cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact
    13801 atgatctatt ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa
    13861 gcagttgcca aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt
    13921 ttcaagcgac caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc
    13981 tgaaaaacac aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt
    14041 cgtattgagg gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga
    14101 tcaagacact ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc
    14161 gaaagttagt gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca
    14221 gatttggcaa tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca
    14281 aatagtagat aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc
    14341 aggtgaaatc tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt
    14401 ttttccaacg attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg
    14461 tcagttgcta gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag
    14521 tgtttacgaa caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca
    14581 taattttaaa gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa
    14641 tcatattcct gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt
    14701 gttacatcga tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta
    14761 tgatctttat acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa
    14821 agaaaaagct ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa
    14881 agcattctct gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta
    14941 ttcttcggga agctatgaca caaatccata tattttattg aattggcatg atacgctgga
    15001 tcagctattt acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc
    15061 gaaccagcct tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac
    15121 gaatgaaaat atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc
    15181 ttatgtactc aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt
    15241 cgctgaattc gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga
    15301 atacctaagt gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga
    15361 agacccagaa attaaattcg aatggtcacg gataccgcat ttttattata attactatgt
    15421 tttccaatac tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca
    15481 ggaaccagag gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc
    15541 tgtggaagta atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc
    15601 aatgtcgatg tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg
    15661 ctttctattt ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg
    15721 gagtcgacgt atttttt
//
LOCUS       DACIFS010000059        15666 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.059, whole genome shotgun sequence.
ACCESSION   DACIFS010000059 DACIFS010000000
VERSION     DACIFS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.059"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            414..947
                     /locus_tag="IYA83_002016"
     CDS             414..947
                     /locus_tag="IYA83_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ8587443.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="IYA83_002017"
     CDS             complement(1437..1961)
                     /locus_tag="IYA83_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587444.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IYA83_002018"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="IYA83_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ8587445.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IYA83_002019"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="IYA83_002019"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ8587446.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="IYA83_002020"
     CDS             complement(3683..4363)
                     /locus_tag="IYA83_002020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ8587447.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="IYA83_002021"
     CDS             4489..5358
                     /locus_tag="IYA83_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587448.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="IYA83_002022"
     CDS             5384..5899
                     /locus_tag="IYA83_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587449.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="IYA83_002023"
     CDS             complement(5946..6509)
                     /locus_tag="IYA83_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587450.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="IYA83_002024"
     CDS             complement(6564..6743)
                     /locus_tag="IYA83_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587451.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="IYA83_002025"
     CDS             complement(6820..7329)
                     /locus_tag="IYA83_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587452.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="IYA83_002026"
     CDS             complement(7425..7805)
                     /locus_tag="IYA83_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ8587453.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /locus_tag="IYA83_002027"
     CDS             8135..8884
                     /locus_tag="IYA83_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ8587454.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="IYA83_002028"
     CDS             complement(9068..10345)
                     /locus_tag="IYA83_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587455.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IYA83_002029"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="IYA83_002029"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ8587456.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="IYA83_002030"
     CDS             complement(11267..12211)
                     /locus_tag="IYA83_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ8587457.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="IYA83_002031"
     CDS             complement(12213..12899)
                     /locus_tag="IYA83_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ8587458.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13372..13692
                     /locus_tag="IYA83_002032"
     CDS             13372..13692
                     /locus_tag="IYA83_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ8587459.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13853..14758
                     /locus_tag="IYA83_002033"
     CDS             13853..14758
                     /locus_tag="IYA83_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8587460.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14755..15528
                     /locus_tag="IYA83_002034"
     CDS             14755..15528
                     /locus_tag="IYA83_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8587461.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT         4584 a         3184 c         2801 g         5097 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttattat
    13021 ataattcacc ttacttattg taagataaag ctttttttaa tttattgcct gctaatatat
    13081 aaaaataagg tctagttact gtttttttaa atgattaata aataacaact cctttctatg
    13141 aaattgcaaa gcattcatct tcttttctgc taacgatata tccgtcaatg cttgttacac
    13201 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
    13261 tatacgatct gccaccgggt agtaaaaaag aggatgatca ttccattagg tctttgaagg
    13321 gatgattctc ctctttttgt gtatttttta tattgaagga ggaacgtatg tatggcatgg
    13381 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
    13441 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggaatgat tgctagtttt
    13501 tactttttgt caaaggcaat caaaagtttg cctatgagtc tctcttatcc tatttggaca
    13561 gggattggcg cagttggttc ggtcattatt ggtgtggtct tgtttcatga taagctgaat
    13621 ctttccactt ggttctttgt cgggttgctg ctcatcagca tcattggcat caaaatcact
    13681 agcggtcact agatacttag acaagcagac aaggactgat cagaaaaagc ggggcttttt
    13741 atttgatatt cctctttcta tgaaaaaaca agaaaataag actaatgacc tagcgacttc
    13801 tctactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag
    13861 tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag
    13921 ttttagctta tctcctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac
    13981 aacgatcatg aaagctattt taggactgat ccattactca gaaggaacga tccgtattgg
    14041 tgaaaatgag atttctccct ctagtcataa aggattagaa caagtaggag cattgatcga
    14101 atatccgggt atctatccat tcttaacagg ttatgatcat ctgaagttat tctctgaaac
    14161 aaacgatgta tctgctatcg acactatcgt gaaacagctc aagatggaaa aatacattca
    14221 caaaaaagcg aaatcctact cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt
    14281 actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc
    14341 aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat
    14401 ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca
    14461 aggaaaaatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat
    14521 cgttatctct acgacaaatg atcaagctgc tcgaacgatc ttgacagacg ctggatatcc
    14581 cttggaagat cctgataaaa tcaagcttct aaaaaaaaac gaagagttat taggaactgt
    14641 tatccagtta ttgtctgcag aaaatgtcca tgtgacagat gtccagcatc tgacgaatga
    14701 tttggaatct tccctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgacg
    14761 ttgcttataa aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct
    14821 gttctattag ttgctttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc
    14881 attgatccag ttgaaatgac tgctcagctt ttcagtgcca catcttggat tgtctttatc
    14941 atgattgctg ctgcaagtac gatcatttca atggaatcac aatatggtac attgaaaaat
    15001 ctcctttatc gaaagtattc tcgaggagaa atattagtaa gtaaatggat cacattagtt
    15061 atttattcta tttatttata ccttttagct attattgtca ccgtgctgat gaaactgatc
    15121 cttttccctt cgatttcctt tacggagaag atttctactg gtcagacact gatccaaagc
    15181 ttattcactt atactttggg tagttatatc ggtttatggc taattttaag tttagtgttg
    15241 atgattgctt gttttatcaa tagttccggt gcctccatct cagcagggat cgtcttttat
    15301 tttgcttcat caatcatatc tggtatcctc atcgcactca ttcaaaaatg ggagtggatc
    15361 aaatggaatc caatcagtat gctgaatctg caaaaccaaa ttggcaacga agagattatg
    15421 aagcaattga ctcatctttc tactaatcaa atgctgtttg gcaatttagc ttacattgct
    15481 ttgttcttag cattgggata cctcgtattc aagcgaaaaa atgtatgatg gttcattaac
    15541 aaagaagttg tgacagaagt ggccagtttc aagaaatttc ggcttatttt tagaagaagc
    15601 tacttcttga acaccattta ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt
    15661 cttttt
//
LOCUS       DACIFS010000060        15244 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.060, whole genome shotgun sequence.
ACCESSION   DACIFS010000060 DACIFS010000000
VERSION     DACIFS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.060"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            168..362
                     /locus_tag="IYA83_002035"
     CDS             168..362
                     /locus_tag="IYA83_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587462.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IYA83_002036"
     CDS             631..930
                     /locus_tag="IYA83_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587463.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IYA83_002037"
     CDS             complement(1027..1461)
                     /locus_tag="IYA83_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587464.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IYA83_002038"
     CDS             complement(1693..2403)
                     /locus_tag="IYA83_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ8587465.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IYA83_002039"
     CDS             complement(2393..3253)
                     /locus_tag="IYA83_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ8587466.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IYA83_002040"
     CDS             complement(3258..3896)
                     /locus_tag="IYA83_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ8587467.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IYA83_002041"
     CDS             complement(3911..4210)
                     /locus_tag="IYA83_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587468.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IYA83_002042"
     CDS             complement(4253..5737)
                     /locus_tag="IYA83_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ8587469.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IYA83_002043"
     CDS             complement(5757..6218)
                     /locus_tag="IYA83_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8587470.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IYA83_002044"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IYA83_002044"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ8587471.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IYA83_002045"
     CDS             7521..8291
                     /locus_tag="IYA83_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8587472.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IYA83_002046"
     CDS             complement(8564..9391)
                     /locus_tag="IYA83_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587473.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IYA83_002047"
     CDS             9959..11194
                     /locus_tag="IYA83_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ8587474.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IYA83_002048"
     CDS             complement(12193..12453)
                     /locus_tag="IYA83_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ8587475.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IYA83_002049"
     CDS             complement(12480..13781)
                     /locus_tag="IYA83_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8587476.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IYA83_002050"
     CDS             complement(14073..14531)
                     /locus_tag="IYA83_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ8587477.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..>15244)
                     /locus_tag="IYA83_002051"
     CDS             complement(14544..>15244)
                     /locus_tag="IYA83_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ8587478.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
BASE COUNT         4876 a         3105 c         2365 g         4898 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcaaatgcgt
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacgac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttat
//
LOCUS       DACIFS010000061        14417 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.061, whole genome shotgun sequence.
ACCESSION   DACIFS010000061 DACIFS010000000
VERSION     DACIFS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.061"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..302)
                     /locus_tag="IYA83_002052"
     CDS             complement(<1..302)
                     /locus_tag="IYA83_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ8587479.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            1419..1892
                     /locus_tag="IYA83_002053"
     CDS             1419..1892
                     /locus_tag="IYA83_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ8587480.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1901..2128
                     /locus_tag="IYA83_002054"
     CDS             1901..2128
                     /locus_tag="IYA83_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587481.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2764..3135
                     /locus_tag="IYA83_002055"
     CDS             2764..3135
                     /locus_tag="IYA83_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587482.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(3391..3633)
                     /locus_tag="IYA83_002056"
     CDS             complement(3391..3633)
                     /locus_tag="IYA83_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ8587483.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3665..4567)
                     /locus_tag="IYA83_002057"
     CDS             complement(3665..4567)
                     /locus_tag="IYA83_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ8587484.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4580..4768)
                     /locus_tag="IYA83_002058"
     CDS             complement(4580..4768)
                     /locus_tag="IYA83_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ8587485.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4782..5345)
                     /gene="amaP"
                     /locus_tag="IYA83_002059"
     CDS             complement(4782..5345)
                     /gene="amaP"
                     /locus_tag="IYA83_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ8587486.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(5383..6276)
                     /locus_tag="IYA83_002060"
     CDS             complement(5383..6276)
                     /locus_tag="IYA83_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8587487.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(6354..7292)
                     /locus_tag="IYA83_002061"
     CDS             complement(6354..7292)
                     /locus_tag="IYA83_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ8587488.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(7326..7676)
                     /locus_tag="IYA83_002062"
     CDS             complement(7326..7676)
                     /locus_tag="IYA83_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ8587489.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7709..8611)
                     /locus_tag="IYA83_002063"
     CDS             complement(7709..8611)
                     /locus_tag="IYA83_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ8587490.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8604..9461)
                     /locus_tag="IYA83_002064"
     CDS             complement(8604..9461)
                     /locus_tag="IYA83_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ8587491.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9795..10604
                     /locus_tag="IYA83_002065"
     CDS             9795..10604
                     /locus_tag="IYA83_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587492.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10644..11141
                     /locus_tag="IYA83_002066"
     CDS             10644..11141
                     /locus_tag="IYA83_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ8587493.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11526..11599)
                     /locus_tag="IYA83_002067"
     tRNA            complement(11526..11599)
                     /locus_tag="IYA83_002067"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11563..11565),aa:Ile,seq:cat)
     gene            11786..12109
                     /locus_tag="IYA83_002068"
     CDS             11786..12109
                     /locus_tag="IYA83_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587494.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            12273..12527
                     /locus_tag="IYA83_002069"
     CDS             12273..12527
                     /locus_tag="IYA83_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587495.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12597..12842
                     /locus_tag="IYA83_002070"
     CDS             12597..12842
                     /locus_tag="IYA83_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587496.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12918..13283
                     /locus_tag="IYA83_002071"
     CDS             12918..13283
                     /locus_tag="IYA83_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587497.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(13344..13907)
                     /locus_tag="IYA83_002072"
     CDS             complement(13344..13907)
                     /locus_tag="IYA83_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587498.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4236 a         2660 c         2383 g         5138 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atggaacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatt ttttcggaga
      481 attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt atttttatat
      541 tatcattttt cttattcact tctttggttt atactattct tataaataaa aacgaggtat
      601 gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat ttgattgtat
      661 caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact actacattat
      721 gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga aaaaaataaa
      781 catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt attcggcaat
      841 ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat aaactaatcc
      901 aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc catgcgtcaa
      961 tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata agtggtgatt
     1021 acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac ttgaaaaaat
     1081 taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct gttaacttac
     1141 acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat aatcccaggc
     1201 atctaaacta gcattataga agagacacac atagaatcta aaataactgg ttttaaatgt
     1261 ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct atcatttttt
     1321 ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa taattattct
     1381 gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa acattgctat
     1441 taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta cttttttata
     1501 ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca ttatttgttt
     1561 ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta ttgaaactat
     1621 tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata ctaaaaaaaa
     1681 tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct aaatttatga
     1741 aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct gtcgtattca
     1801 ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa catgcgattt
     1861 tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta tcgttaattt
     1921 agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga aagtgggcat
     1981 tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc gtttttccac
     2041 tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga cctataaaaa
     2101 tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa ataaaaaata
     2161 atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat tcagattttt
     2221 tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca tacaaagggg
     2281 gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct gttacgttgt
     2341 agttggatct gcttttagta taatacttta ttcattccac cagactgata gcagtttgag
     2401 cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga agtgcttaaa
     2461 acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt aagctcccat
     2521 atatactgta aagccttcta tatattatgt catgttgctt tttaacattt actatgtagt
     2581 tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt aacattattt
     2641 tgataataat gattatttat ttaaaaatac attagataac atttgttacg acacgaaaaa
     2701 gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat taggaggaat
     2761 agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat tccattacca
     2821 acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc tgctcttacg
     2881 aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta ttggctaaaa
     2941 cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct ctttgagttt
     3001 ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat catctgggtt
     3061 ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa atttagggag
     3121 gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag gaaaaacttt
     3181 taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa gtcttgtcac
     3241 agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat atgcccaacc
     3301 aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgaagaacag
     3361 ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga atgatacgat
     3421 tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg caatacttgg
     3481 tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg ctacgacatt
     3541 tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg ctccgataat
     3601 tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat gttttacttc
     3661 cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg cttccagctg
     3721 ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc gctgcgtcag
     3781 ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt ttgatgtcca
     3841 ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact tctttgccga
     3901 ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc acaatgacat
     3961 ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc gttacattat
     4021 ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca gataataaac
     4081 cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct tcataattca
     4141 gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta tttgtatgat
     4201 cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct tgtgctttcg
     4261 catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt gcatcatgtt
     4321 gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt tctgcatcat
     4381 gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga tgtgcagata
     4441 ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt gtcgttgggt
     4501 ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt ttgttattgt
     4561 tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc gtttgtttca
     4621 gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag gtcttgaaaa
     4681 agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag atgataggga
     4741 gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac tcgtttgcgg
     4801 tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac tttcactggc
     4861 tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa tgttcctgtt
     4921 ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc gatcttgttt
     4981 ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg caagtgagaa
     5041 cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc atgtttcagt
     5101 agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat aattgtcaaa
     5161 atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt agtgatggaa
     5221 aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa tagtatcgca
     5281 aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt tacaccttta
     5341 ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt tgatcgcttc
     5401 tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg atgctaagaa
     5461 aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac tttgctgacc
     5521 aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg tccagattgg
     5581 accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg ctaaaccgat
     5641 cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt cgcttggatt
     5701 gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat gcggaacagc
     5761 ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt tgacttgctc
     5821 aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta gtaccatcgt
     5881 atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg cttgttcgtc
     5941 acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt attttgctac
     6001 ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg ccccttctct
     6061 agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga ttagtacacg
     6121 gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag gaacaggaat
     6181 catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct ccgtatggta
     6241 caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc tagtttttaa
     6301 attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat tctttaattt
     6361 gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa agagccaaag
     6421 actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt attcattccc
     6481 caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc tagatatttc
     6541 atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat actctctaat
     6601 aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg gacatcatag
     6661 agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa ttgatccatt
     6721 gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc aagatctgcc
     6781 tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa agcgtcaatt
     6841 cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa taatgtatac
     6901 gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag ccgatccaca
     6961 aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac aaaagatatt
     7021 ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac taccaaagaa
     7081 aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc ttccggatat
     7141 tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg ctcaatctct
     7201 tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt ttctattaca
     7261 ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg actttctcga
     7321 aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac tgcgatttca
     7381 ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg tttaccgaaa
     7441 taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat tttagtcgca
     7501 tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt tgcaaaagca
     7561 cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat ttttgtattt
     7621 tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat ttccattcct
     7681 actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt tttggttgaa
     7741 cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc gtcatccctg
     7801 catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac ggtttcacat
     7861 gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca cgttcacctg
     7921 ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc caacgcggat
     7981 gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca tggaatcgta
     8041 atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt tcatctaacc
     8101 attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat ttagctgaaa
     8161 ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct acgattgaat
     8221 caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg tcgcaatcca
     8281 tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca atacgataat
     8341 aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct ttattgattt
     8401 tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt aaaagctgtt
     8461 tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta gacgggttgc
     8521 aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt gtgttacaac
     8581 ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt tagagtactg
     8641 tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt attccaaggt
     8701 ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt atactgacgt
     8761 tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg catgttccat
     8821 tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc ttggtcgtga
     8881 tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt aatttctttt
     8941 ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa gtcatcggaa
     9001 tcgattccta atctttccaa agctttcact tggcctggat cgataatagc gccgattgtt
     9061 tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa cacatctgaa
     9121 tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa agaaattctt
     9181 aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat atctttttcc
     9241 actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc ttccaatcct
     9301 tctttactat attctgatag accatcgtca atcacataaa attttatgcg tcttgtttta
     9361 ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata attttcgtct
     9421 gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac tcctttttct
     9481 aatctttttc taacctgttc tacatctaga atattcctca tttggtttta aagcaaaata
     9541 tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca tcggtaagcg
     9601 ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac tactatatat
     9661 agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag caagttagtt
     9721 tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat gtaaaaagaa
     9781 agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg cttgatcgaa
     9841 atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa aatggcggaa
     9901 tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt tccgaattag
     9961 gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg aaggatggaa
    10021 aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga aaagccttct
    10081 tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa gatgatcagc
    10141 tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat ttcaatttac
    10201 agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt agctatctta
    10261 aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga gatctgcaag
    10321 cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg cctacaggta
    10381 tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa tcgagtgaca
    10441 tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc gggttgagta
    10501 ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta acactgacga
    10561 atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa taaaatgacg
    10621 atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat catattcttg
    10681 atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt attgctcaaa
    10741 gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat ataccagttg
    10801 aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt ctttctccac
    10861 aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa actttagctg
    10921 aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa gtagtcggga
    10981 ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct tttcctgaag
    11041 cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa tccgctttag
    11101 agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta tcttcattta
    11161 cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac gtgctttttc
    11221 tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca cgacctcttt
    11281 cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc aaaatatgag
    11341 gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt cacaacctct
    11401 tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa gtaaaaacat
    11461 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    11521 tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa
    11581 ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt caactatagc
    11641 acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt tattcttatt
    11701 tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta ttttagaaaa
    11761 aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat ttattgcgat
    11821 tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc aaacctcagg
    11881 aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc ttgtcatgat
    11941 gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg ttaaatatgt
    12001 ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg tcctcttttt
    12061 atatgtattg aattacttag atatcaatcc attgattttt tctcgataga taagacacat
    12121 tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat ttttcattga
    12181 tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa catgttatct
    12241 tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga gttttgtttt
    12301 gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg aacaaagaac
    12361 ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa gtttttaatt
    12421 acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg gttttgtctt
    12481 tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta ctattgattg
    12541 catgtgtagc tttctatagt cattataact attctactat aatgaaaggt gattaaatga
    12601 gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc ttaagaactg
    12661 gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta ttcaaatatc
    12721 gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat atcgctgact
    12781 tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc aaactgaagt
    12841 gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa caatctatat
    12901 ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag cttcctcgtc
    12961 aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc tggggaaaga
    13021 aacatgctac tgtggattat gtttttcact attctcctta tagattattc caatatcatc
    13081 agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag tggaaaaatc
    13141 ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta gagcgcaacg
    13201 tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca aatttggagc
    13261 aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct cttacttcat
    13321 taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg gttatcttca
    13381 tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac ttccctagac
    13441 ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc tgtatacgat
    13501 gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct tcttctacac
    13561 cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc catgcatcgt
    13621 cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc tgtcgtggct
    13681 tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc tgccattgct
    13741 agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc ctgaaaatag
    13801 actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat ttctaaatat
    13861 tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt tatttttaaa
    13921 aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa aaaaattatt
    13981 acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct ttcatcactt
    14041 gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg tattccttca
    14101 aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact taaaaattta
    14161 gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat tgacagacca
    14221 atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt cattttcaag
    14281 acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta ttcgtatttt
    14341 cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta ttcagcccat
    14401 tcaacgacca atgaatt
//
LOCUS       DACIFS010000062        14078 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.062, whole genome shotgun sequence.
ACCESSION   DACIFS010000062 DACIFS010000000
VERSION     DACIFS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.062"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            337..2502
                     /gene="acm"
                     /locus_tag="IYA83_002073"
     CDS             337..2502
                     /gene="acm"
                     /locus_tag="IYA83_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ8587499.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2774..3259)
                     /locus_tag="IYA83_002074"
     CDS             complement(2774..3259)
                     /locus_tag="IYA83_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ8587500.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3739..4380
                     /locus_tag="IYA83_002075"
     CDS             3739..4380
                     /locus_tag="IYA83_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ8587501.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4399..5034
                     /locus_tag="IYA83_002076"
     CDS             4399..5034
                     /locus_tag="IYA83_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587502.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5052..5867
                     /locus_tag="IYA83_002077"
     CDS             5052..5867
                     /locus_tag="IYA83_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8587503.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6186..7622
                     /locus_tag="IYA83_002078"
     CDS             6186..7622
                     /locus_tag="IYA83_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8587504.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7811..10258
                     /locus_tag="IYA83_002079"
     CDS             7811..10258
                     /locus_tag="IYA83_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ8587505.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10488..11555
                     /gene="rlmN"
                     /locus_tag="IYA83_002080"
     CDS             10488..11555
                     /gene="rlmN"
                     /locus_tag="IYA83_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ8587506.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11748..12851)
                     /locus_tag="IYA83_002081"
     CDS             complement(11748..12851)
                     /locus_tag="IYA83_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ8587507.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13404..13952
                     /gene="aac(6')"
                     /locus_tag="IYA83_002082"
     CDS             13404..13952
                     /gene="aac(6')"
                     /locus_tag="IYA83_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ8587508.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4845 a         2311 c         2966 g         3956 t
ORIGIN      
        1 tagtataaac taataatgtt aattaaaagt gtattttatg aaactatcgt tatttattaa
       61 tgttgcattt ttgtaagtta aaaattggaa atatagattg aaaaaatgaa agcaataagt
      121 aaaataaaat gaatatatgc gaaaaaataa tttacattag gaaaaacctt gtaaaaacag
      181 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
      241 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      301 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      361 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      421 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      481 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      541 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      601 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      661 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      721 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      781 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      841 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      901 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      961 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
     1021 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
     1081 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
     1141 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1201 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1261 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1321 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1381 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1441 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1501 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1561 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1621 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1681 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1741 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1801 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1861 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1921 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1981 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     2041 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     2101 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     2161 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2221 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2281 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2341 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2401 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2461 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2521 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2581 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2641 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2701 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2761 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2821 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2881 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2941 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     3001 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     3061 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     3121 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3181 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3241 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3301 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3361 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3421 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3481 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3541 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3601 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3661 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3721 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3781 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3841 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3901 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3961 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     4021 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     4081 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     4141 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4201 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4261 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4321 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4381 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4441 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4501 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4561 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4621 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4681 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4741 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4801 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4861 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4921 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4981 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     5041 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     5101 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     5161 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5221 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5281 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5341 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5401 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5461 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5521 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5581 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5641 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5701 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5761 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5821 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5881 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5941 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     6001 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     6061 tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt
     6121 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6181 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6241 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6301 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6361 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6421 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6481 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6541 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6601 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6661 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6721 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6781 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6841 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6901 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6961 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     7021 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     7081 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     7141 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7201 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7261 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7321 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7381 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7441 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7501 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7561 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7621 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7681 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7741 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7801 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7861 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7921 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7981 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     8041 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     8101 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     8161 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8221 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8281 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8341 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8401 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8461 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8521 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8581 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8641 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8701 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8761 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8821 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8881 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8941 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     9001 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     9061 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     9121 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9181 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9241 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9301 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9361 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9421 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9481 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9541 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9601 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9661 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9721 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9781 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9841 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9901 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9961 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
    10021 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
    10081 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
    10141 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10201 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10261 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10321 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10381 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10441 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10501 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10561 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10621 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10681 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10741 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10801 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10861 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10921 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10981 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    11041 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    11101 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    11161 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11221 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11281 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11341 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11401 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11461 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11521 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11581 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11641 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11701 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11761 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11821 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11881 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11941 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    12001 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    12061 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    12121 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12181 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12241 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12301 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12361 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12421 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12481 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12541 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12601 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12661 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12721 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12781 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12841 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12901 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12961 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    13021 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    13081 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    13141 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13201 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13261 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13321 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13381 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13441 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13501 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13561 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13621 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13681 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13741 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13801 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13861 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13921 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13981 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    14041 cttatttccg aagaagctac ttttggaaca ccgtgtac
//
LOCUS       DACIFS010000063        14026 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.063, whole genome shotgun sequence.
ACCESSION   DACIFS010000063 DACIFS010000000
VERSION     DACIFS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.063"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            807..1007
                     /locus_tag="IYA83_002083"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IYA83_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IYA83_002084"
     CDS             1494..1673
                     /locus_tag="IYA83_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587509.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IYA83_002085"
     CDS             complement(1719..2324)
                     /locus_tag="IYA83_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587510.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="IYA83_002086"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="IYA83_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3006..3233
                     /locus_tag="IYA83_002087"
                     /pseudo
     CDS             3006..3233
                     /locus_tag="IYA83_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3359..4330
                     /locus_tag="IYA83_002088"
     CDS             3359..4330
                     /locus_tag="IYA83_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8587511.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="IYA83_002089"
     CDS             4470..5783
                     /locus_tag="IYA83_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ8587512.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="IYA83_002090"
     CDS             5826..6692
                     /locus_tag="IYA83_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ8587513.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="IYA83_002091"
     CDS             6689..7537
                     /locus_tag="IYA83_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ8587514.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="IYA83_002092"
     CDS             7551..9023
                     /locus_tag="IYA83_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ8587515.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..9912
                     /locus_tag="IYA83_002093"
     CDS             9040..9912
                     /locus_tag="IYA83_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ8587516.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(10146..11527)
                     /locus_tag="IYA83_002094"
                     /pseudo
     CDS             complement(10146..11527)
                     /locus_tag="IYA83_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11817..12422
                     /locus_tag="IYA83_002095"
     CDS             11817..12422
                     /locus_tag="IYA83_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ8587517.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            12438..13010
                     /locus_tag="IYA83_002096"
     CDS             12438..13010
                     /locus_tag="IYA83_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587518.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(13483..>14026)
                     /locus_tag="IYA83_002097"
     CDS             complement(13483..>14026)
                     /locus_tag="IYA83_002097"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ8587519.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
BASE COUNT         4387 a         2370 c         2833 g         4436 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtgaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaactttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaacgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgttttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggcaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 agaaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttctaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaatacctt tagtcattga cttcttcaaa
     9181 caataccaag cagatttagc agggattggg attggctcat ttgggcctat cgatatccat
     9241 cgcgattccg ccacttatgg gtacattacc tcgaccccga aagtggcatg gcaaaatttt
     9301 gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc
     9361 aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt
     9421 tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa
     9481 gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt
     9541 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
     9601 gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc
     9661 gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg
     9721 atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca
     9781 tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc
     9841 ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc
     9901 ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac
     9961 tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa
    10021 gatcaagcct tgaatgagat aagttcttgc tcgaaagtta tggaaatttc gatagccgta
    10081 ggccacttgt attgtgcaat agttttttac aaaaaaaggg ggtgcggata tttttttgga
    10141 cctttttaac aaatatatct ttgcttcttc cgatattgaa gagggcttag ccctcctaaa
    10201 gataacttaa ttcgttgatt attataccaa tgaatataat catccaattt gtttataaac
    10261 tgattgattg ttgtattctt ccagtcacgt tgataaaaca tctcgttttt taatcgtcca
    10321 aagaaacctt cgcacgctga attatctggt gaataaccct ttttagacat agagtgaagc
    10381 aagttggctt tgttccaata gtccaactaa caatagcacc atcgtaacaa tcgacaagtg
    10441 gtgataagta gattttccct gcgggaattc taaattcagt aatatctgtt aaccattttt
    10501 catttggctt ctttgcttta aaattccgat ttaataaatt ggggtgtgca ggagaaattt
    10561 ccccagcata cgaagaatac ttttttcgtc tagaaaattt aacaaataga ttttcctcag
    10621 tcatcaatct acgtacaact ttttcagaaa gctttttacc catatttttt aaagcttggt
    10681 gaattctacg gtaaccatac gcacagaagt tatgattgaa aatcgtgata attaattgac
    10741 gttctttgta atacttatct ggtttctcaa gtgcttgttt ttgataaaaa tagctgcttt
    10801 taggcaactg gagctgttga agaagctctt tcaaggtgaa ctgatttcgt aaggcatcaa
    10861 tcaatatcgt tttttcttga tttgatagac aaagaagatt gacgcctttt tcttttttta
    10921 gcagttcagc cgctccttcg agtaatgttt tttcgagctg taattggtgt acttgttttt
    10981 gtaagtcttc tacttccttt aaaagtagct ctttttgttt ttcctgattt tttaaagtca
    11041 tacgcaatgg aaaatccttt ccaagaaccg tgtttttcca ttgatataga gtaactctac
    11101 taattccttc agattctgct aaatttttcc tattagtttg cggtttatag aattttttca
    11161 acacagcttc tttttgatct tgtgttaact ttaccgcact ttttcggagt tttctagctc
    11221 ctggtgcaag ttcttcacac cgctgccgca aggactctct attaggataa ccaagcatgc
    11281 gaatggttcg tgcataacat tgaccatatt caaaataatg attcaccgct attcgttttt
    11341 gttcttcaga atactttgat ttttttcggt gctcctgatg gagctctccg gattccaaat
    11401 actctttata ccatttccgt agtgcaccaa tcgacggata ccctaattct cgaatggttg
    11461 cagcataaga gcaatcgtat tttaaaaata actgaattgc ccttacacgc tctttgtggg
    11521 aaaacacacg gacattcctc ctaagtttat tttttcgtcc aggattatgt ccgcaccccc
    11581 ttgctttctg atgacttatt ctggttcttt gagtgtcaat gcatagatgt gatgtagcta
    11641 gttagaaaaa aattttgaat actctttttt tctttcagac tcctttttaa gtaagatttt
    11701 gaagtgtgag ttacggggtc agaaacttaa tagtttttga cacataaaca aaatcgcgat
    11761 ataatggggt tgagagtgtt tcataaactt atgtcataaa gttaaggaga tttcgcatga
    11821 ttttagagaa taaattggga ttaaccaatc aagttgagtt ggctaaggtt gaagaaaaac
    11881 tcagtaaaca aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa atcgaagttg
    11941 gtacatttaa aggtttatct gaaattcatg aatttctatt ttcggatatt tacgattttg
    12001 ccggaaaaat ccgatcggtc aatattgcaa aaggaaattt tcgatttgca ccagttatgt
    12061 atttagaaca ttcgttacag catattgatc agatgccaca aacttctttt gaagagatta
    12121 tcaagaaata tgtggagatg aatatcgctc acccatttcg agaaggaaat ggccgaagta
    12181 ctagaatttg gctggatctc attttaaagg aagaattgca aaaagttgta gattggaact
    12241 taatcgataa agcagactat ctttctgcaa tggagcgaag tcccataaac gatttggaaa
    12301 tacgctatct aatttccaat gctctgactg ataaaattga cgatcgcgag ttatatatga
    12361 aaggaatcga tgtcagctat ttctatgaag ggtattctga gtatacgata gatgatttgt
    12421 aaaagtaggt ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc gcttctcaaa
    12481 gtacaaaaga acaaattcaa cagttgcttg agaacggcgt agagaagaag aatatttttt
    12541 cagaaaaatt tacgggtaca aaacgggatc ggccaaaatt caatcagctg ttggctggtc
    12601 tgaagcctgg agatgaaata gttgttacga agcttgatcg atttgctcgt aacacgagag
    12661 aagcattaga gatcattgaa cctttactcg atgataacgt aacgatcaag gttttgaatc
    12721 taggaacgat tgaaaatacg tccatggggc gaatggtgac acggacgttg ttgagtgtag
    12781 cagaaatgga acgtgatatg attgtggaac gtacccaaga aggaaaaatg tttgcaagaa
    12841 agaataatcc taatttcaga gaaggacgtc cgaaagcaac aatcacaccg aagaagaggc
    12901 acgcatatga actgcttact tcaggtaaat cgtataagga agttgaagct ataaccggtt
    12961 tcagtcgcag tacgcttttt agaataaaga agaaaattga agaaagttaa aaaagaagtc
    13021 tcagcgaacc agacaggtaa atacttaatg cgttctagat atgagtctat tagcatgttg
    13081 tgaaatataa tattttttta tttacttgat ttctgctcaa aaaatgtgta tggtaactgt
    13141 aaaaggtaaa gtaattaaag gtatgctagc cacgcttact atattaggat accatcatat
    13201 atttataatg aacgatttgt tcgtctacca aacaacacat atcgttcagc gtaagtcacg
    13261 tttaggaacg tggctttttt agtgttatta tcgataaata atctattgac tctattgatt
    13321 ttatctccat atccctacgg tgttgattgt ctttagatct ttcatttaag aaccaaataa
    13381 ctatcataaa aatcagcact gctgctaata taataaacag cattcctatt gtgatattac
    13441 tggatttgag tcaatatctt ggacacattt tagtagagac ggttaagcca ttttttcttt
    13501 tgctaattct tcaaattcct gtggggttag atagtgaatc gagctatgga ttcgatttcg
    13561 gttataaaat ccttcaatgt agctaaatag cgctcgattt gcttcttcaa aatctgagta
    13621 gctagtcgtg tagacttctt cttttttcaa tgaggcatgg aaagattcga ttccagcatt
    13681 atcataaggt gttccttttc tactataaga atgccttatt ttgttggttt taagccattg
    13741 ttccacttct cttgctgtgt attggctgcc caagtcagtg tgtagaatca ttccttctgg
    13801 aatgtgataa tgtatttttg ctttattcaa tgtttgaatg acacaatcca cagtcattcg
    13861 ttttgaaaat gtataactaa taattctttt cgtgtgtaaa tccatgattg aagataagta
    13921 acaccaacca tttttcttag taggaatgta cgtaatgtca gctgcccatt tctcacagat
    13981 cgtttcagta gaaaagtcct gatttaagag attctctttt gaaatg
//
LOCUS       DACIFS010000064        13969 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.064, whole genome shotgun sequence.
ACCESSION   DACIFS010000064 DACIFS010000000
VERSION     DACIFS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.064"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IYA83_002098"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="IYA83_002098"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ8587520.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="IYA83_002099"
     CDS             complement(2424..2675)
                     /locus_tag="IYA83_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ8587521.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="IYA83_002100"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="IYA83_002100"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ8587522.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="IYA83_002101"
     CDS             3624..3842
                     /locus_tag="IYA83_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587523.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="IYA83_002102"
     CDS             complement(4130..4642)
                     /locus_tag="IYA83_002102"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ8587524.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IYA83_002103"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="IYA83_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ8587525.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7529)
                     /locus_tag="IYA83_002104"
     CDS             complement(7095..7529)
                     /locus_tag="IYA83_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ8587526.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="IYA83_002105"
     CDS             complement(7539..8327)
                     /locus_tag="IYA83_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8587527.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="IYA83_002106"
     CDS             complement(8324..9265)
                     /locus_tag="IYA83_002106"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ8587528.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="IYA83_002107"
     CDS             complement(9442..10029)
                     /locus_tag="IYA83_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ8587529.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IYA83_002108"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="IYA83_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ8587530.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="IYA83_002109"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="IYA83_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ8587531.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="IYA83_002110"
     CDS             complement(12946..13881)
                     /locus_tag="IYA83_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587532.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DACIFS010000065        13937 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.065, whole genome shotgun sequence.
ACCESSION   DACIFS010000065 DACIFS010000000
VERSION     DACIFS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.065"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            131..505
                     /locus_tag="IYA83_002111"
     CDS             131..505
                     /locus_tag="IYA83_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ8587533.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IYA83_002112"
     CDS             complement(619..1248)
                     /locus_tag="IYA83_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ8587534.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IYA83_002113"
     tRNA            complement(1382..1454)
                     /locus_tag="IYA83_002113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IYA83_002114"
     tRNA            1668..1741
                     /locus_tag="IYA83_002114"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IYA83_002115"
     CDS             1696..1845
                     /locus_tag="IYA83_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587535.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IYA83_002116"
     CDS             2338..3666
                     /locus_tag="IYA83_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ8587536.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IYA83_002117"
     CDS             3868..4701
                     /locus_tag="IYA83_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ8587537.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IYA83_002118"
     CDS             4717..5454
                     /locus_tag="IYA83_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ8587538.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IYA83_002119"
     CDS             complement(5609..6577)
                     /locus_tag="IYA83_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ8587539.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IYA83_002120"
     CDS             6809..7642
                     /locus_tag="IYA83_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ8587540.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IYA83_002121"
     CDS             7676..8515
                     /locus_tag="IYA83_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587541.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IYA83_002122"
     CDS             8541..9101
                     /locus_tag="IYA83_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ8587542.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IYA83_002123"
     CDS             9300..11021
                     /locus_tag="IYA83_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ8587543.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IYA83_002124"
     CDS             11186..12328
                     /locus_tag="IYA83_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ8587544.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IYA83_002125"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IYA83_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ8587545.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4757 a         2305 c         2837 g         4038 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatc
//
LOCUS       DACIFS010000066        13720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.066, whole genome shotgun sequence.
ACCESSION   DACIFS010000066 DACIFS010000000
VERSION     DACIFS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.066"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            54..506
                     /locus_tag="IYA83_002126"
     CDS             54..506
                     /locus_tag="IYA83_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587546.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="IYA83_002127"
     CDS             520..1209
                     /locus_tag="IYA83_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587547.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="IYA83_002128"
     CDS             1334..3685
                     /locus_tag="IYA83_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ8587548.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="IYA83_002129"
     CDS             3765..4160
                     /locus_tag="IYA83_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587549.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="IYA83_002130"
     CDS             4485..5102
                     /locus_tag="IYA83_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587550.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="IYA83_002131"
     CDS             5143..5442
                     /locus_tag="IYA83_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587551.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="IYA83_002132"
     CDS             5476..5643
                     /locus_tag="IYA83_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8587552.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6293)
                     /locus_tag="IYA83_002133"
     CDS             complement(5688..6293)
                     /locus_tag="IYA83_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587553.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6418..9405
                     /locus_tag="IYA83_002134"
     CDS             6418..9405
                     /locus_tag="IYA83_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ8587554.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            9554..9763
                     /locus_tag="IYA83_002135"
                     /pseudo
     CDS             9554..9763
                     /locus_tag="IYA83_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9898..10074
                     /locus_tag="IYA83_002136"
                     /pseudo
     CDS             9898..10074
                     /locus_tag="IYA83_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            10056..10412
                     /locus_tag="IYA83_002137"
     CDS             10056..10412
                     /locus_tag="IYA83_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587555.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(10926..11093)
                     /locus_tag="IYA83_002138"
     CDS             complement(10926..11093)
                     /locus_tag="IYA83_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8587556.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IYA83_002139"
     CDS             complement(11181..13121)
                     /gene="tet(S)"
                     /locus_tag="IYA83_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ8587557.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            complement(13122..13208)
                     /locus_tag="IYA83_002140"
                     /pseudo
     CDS             complement(13122..13208)
                     /locus_tag="IYA83_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
BASE COUNT         4618 a         2122 c         2496 g         4484 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gatgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat tgccaattgt
     6241 cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc cataaaaaac
     6301 tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta ctgctatcat
     6361 agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag ggtttaaatg
     6421 gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc tagtacagaa
     6481 caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga gactatacga
     6541 cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt tttaggaact
     6601 tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc aactcaacta
     6661 aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta tcagcacaca
     6721 aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat agaaacatac
     6781 ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga tatgttgttt
     6841 gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat tactactttt
     6901 tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg acaattgtct
     6961 ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt aattggaact
     7021 ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc tgacgatagt
     7081 cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt ctctactgaa
     7141 aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa ttatgcattt
     7201 aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc gttgttagtg
     7261 gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc actatctaat
     7321 ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg tcttcgtacc
     7381 ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt agtaatgtcc
     7441 agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa atattctgag
     7501 gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga tcgtgaacca
     7561 atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat tccggatatt
     7621 ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga actctggacc
     7681 ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc tattaaacat
     7741 actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac taataaatgt
     7801 gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga tatttatatt
     7861 cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa aacttgggtt
     7921 caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa tgaaatgtct
     7981 ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg ttggcctact
     8041 tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact taaaaagaca
     8101 tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact tcctaaaatt
     8161 gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca atcatttaac
     8221 cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt agcagttctt
     8281 ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt tgattcttta
     8341 aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac actttcagga
     8401 gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat atggggtgat
     8461 gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc cctatattct
     8521 agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa ttttgtctct
     8581 gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga ttcgctttac
     8641 cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat catgactgat
     8701 acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt tcaatttagt
     8761 ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa cgctcactac
     8821 ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta tacccattgg
     8881 gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc aacagaatta
     8941 acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat aactgagtat
     9001 ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat atatgcacgg
     9061 caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg taatattttt
     9121 tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga gcagctcaat
     9181 gcattaggat tagttactaa tgccatcata tattggaact cactgtattt agaaaaagta
     9241 tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg caggcaatct
     9301 ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata cgaagctgaa
     9361 ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg ttcgggtttt
     9421 gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat agaaaaagag
     9481 aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg tgctaccgca
     9541 ctatttcgtt tctatgttcc atgtacccct ttatcaagaa gcgcaaacac gccaaattgc
     9601 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     9661 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     9721 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     9781 ttactaatag tctttcctaa cattgattta atagtcttta aagggttctg ttgcaaagtt
     9841 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
     9901 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
     9961 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
    10021 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
    10081 ttgatttcaa accattgggt cttgcgatta aagaagcccg tcttagtaaa ggattgactc
    10141 gtgagcaggt aggatcaatg atccagattg cgccacgcta tttgacgaac attgaaaata
    10201 aaggacaaca gccaagtttt catgttgtat atgatttagt gactttgcta aatatttctt
    10261 tggatggatt tattttaggg gaagaaaaca gtcataaaag ctccaaacgt cttcaagtag
    10321 aagcacagtt ggattgtctg gatgaagatg aattagtgac gattgggaaa gtgattcaag
    10381 caatttacgg agaaaaatgt aaatttaact agttcaataa tacacaatgg attaattaat
    10441 actatcagga aagattgatt acaaagatgt aatgaatttt ttctgatttt tgttaatcat
    10501 aaatagaaaa tgaatttatc tttgttacac ttaggaacta gagcgaatga taaatagttc
    10561 tttggttact cttatcgctg caaagtacag ttcatttttt gttgttggga aaattttctg
    10621 gatttattgc atatcacttc atccgactaa aaatttttga ttcccatagc gaagttcgtt
    10681 tttgttcctt aggaaaaact tgactgatat actagttttg aaatgacgag taactctgta
    10741 cttcatcaat aatcaagtat tgaattttaa ttgtatactt tttatgcttg agggatgtct
    10801 actgtaatac agctactgca aaaaaaatca gtacacttat tttcttataa aggatatcaa
    10861 gaactaagct caatggtatg catagaaaag aaaaagccga taaaatgaga ttttgatatc
    10921 tcgaattatc ggctctgcgt ctttgcgtct ggctctgtaa ttacagttat tctaaacttc
    10981 gttatttcaa ttaaaatttc ttgtctgcat ttaggacaaa atagagggaa attttttaat
    11041 tcagtatctt ccctcatttt taaacgggtc ttattgccac atatagggca cagtatccac
    11101 ctagtattta caatatctat atcctcctgt tctaattaac aactcaatat tcattttaaa
    11161 aatttctctt tagttcacta tcaatgtaag ttgattttat taaacatatg ccttactttg
    11221 tctattctat tatttggacg acgtggttgg aaagcagact taatgttagt aacctgataa
    11281 ccttttaact ctgttaaaca gacactgcgt ccatttgtaa agaaagttaa actgtttcga
    11341 tactcttgaa tacaacgggc tggaatttca ccaatgagaa tgacctcgtt accttttaac
    11401 ttagtattta aaatatttgc acaatatttg gaagcatcat tatatgctct cgaaagatat
    11461 tcttgtggta cataaatttc gaagctaaga tatggctcta ataactctgt accactcttt
    11521 ctaaaagcct gctctagtac aataggcgca agcattcgga aatctgctgg cgtactgaca
    11581 gggctgtaat atagaccata cttaaaacag atcttacagt ctgttaattt ccaaccgtac
    11641 aatccttgtt cacacccata gcgtatacct tccataactg cattttgaaa tgattgattt
    11701 aaataaccta gagaaaccag gctctcatac tgaatgccac tacccaaagg aagtggtgtt
    11761 acagataaac caatagaagc ccagaaagga tttggaggta cttcgatatc aatggtaaat
    11821 tcagattttt ttaatggtct ttccatataa atgacagttg gttttcttgt ttctatctca
    11881 atatgatatt tttcttgaat cagagtacaa gttacctcca tttggacctc acctaaaaaa
    11941 gatagcacaa tttcgtgagt tactgtatct acataatatt gtagaagggg atcactatcg
    12001 gatatttcaa aaagtgcatc tagtaacttt tctctttgta ctgatttaca tggttcaatt
    12061 gttgtttgta acataggaag aggattctca agaatttctc tatgtggtaa tcttttttta
    12121 tctccaagta cgttatttag ttttaaaagc tcatttttta aaataataat ctcaccaggc
    12181 tctgccttat ctatctggcg taattctcca tttattgaag tatacatttc tgtaactttt
    12241 attttttcct tttctgatat attgactgag tctcgcaaat gtagcgttcc actataaaga
    12301 cgtacataga caagccgttg accatcatct gaatattcta ctttaaaaac atttccacaa
    12361 agtttatctg aattgagttg tgtgggtgaa aataatttgc tagtaattac ctctataagt
    12421 tgtttaattc caatattatt ctttgcactt ccgtgataaa caggatataa ggagcaactt
    12481 tgaattcttt cgttttcctc cttttcaagt tctgcaatat tcaatgtttt cccaatggta
    12541 tatttttcta ataaataatc atttcccaca attactgtct cccattgttc tggttcagta
    12601 taatctatta cataaggttt caaatttaga ttcacagtct gcttaattat gatgtcgtca
    12661 gaaagtttgt ccttaatatc ttgataaaca tctggcaaat ttattccatt ttgatcaatt
    12721 ttgttaataa aaaatattat aggtatgttc atctttctaa gtgcatggaa tagtatacga
    12781 gtttgtgact gtactccatc ttttgcagag attagcaaaa tagctccatc caaaacagat
    12841 aatgaacggt atacatctgc caaaaaatcc atgtgtccag gagtatctac aatatttact
    12901 ttaacatttt cccgttgaaa agatgttatt gcggtctgaa tagtaatacc tctctggcgt
    12961 tccaaaaaca tagtatccgt tttcgttgta ccgctatcta cacttcctaa ctctttaatt
    13021 gctccgctac tgtatagtaa gctttctgtc aaagtagttt ttcctgcatc aacatgtgct
    13081 aagataccga tattaataat tttcaatttt atttcctcca ttcaaaagct caaaaagcag
    13141 aaaaaatccc agtgataaat actcttatca ctgggatttt aatacaaaaa tagctgtata
    13201 ctacacactg atagccacag atgctttatg ataaaatgat aaaagtattc ttaaactggg
    13261 cacaaaaaac cagtccttat tttaaataag gatgcttaat taattattcc cgctatctaa
    13321 ttgacagttt atttaagaat accttgccgc atttttatta tctccttttg aatgttatct
    13381 taattctatc atatgataga atgcttgtaa agccaaccat attttttgaa cgaaagggaa
    13441 attaatgaga aattaatgga agttagtgta gtagttgaag ataactcttc agaatggcta
    13501 tcaaaattca gcgatttaat aacgatttac aagttcataa gggattcttt attcatccag
    13561 ctattatatt acttttcagg tcaataggac aactgtccac aacctttcta caaatatatt
    13621 acgtataatt tttgaaatat atactgctta acaaaatatt ttgcagtggt tccaatagtt
    13681 ttgaattgtt aatattaagt ttgacttgga aaatatcatt
//
LOCUS       DACIFS010000067        13646 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.067, whole genome shotgun sequence.
ACCESSION   DACIFS010000067 DACIFS010000000
VERSION     DACIFS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.067"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..587)
                     /locus_tag="IYA83_002141"
     CDS             complement(<1..587)
                     /locus_tag="IYA83_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587558.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="IYA83_002142"
     CDS             complement(735..1277)
                     /locus_tag="IYA83_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ8587559.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="IYA83_002143"
     CDS             1412..1801
                     /locus_tag="IYA83_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587560.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="IYA83_002144"
     CDS             1805..2569
                     /locus_tag="IYA83_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ8587561.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="IYA83_002145"
     CDS             2698..3645
                     /locus_tag="IYA83_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ8587562.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="IYA83_002146"
     CDS             3775..4515
                     /locus_tag="IYA83_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587563.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="IYA83_002147"
     CDS             complement(4806..5474)
                     /locus_tag="IYA83_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8587564.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="IYA83_002148"
     CDS             5608..5895
                     /locus_tag="IYA83_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587565.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="IYA83_002149"
     CDS             6311..7849
                     /locus_tag="IYA83_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ8587566.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="IYA83_002150"
     CDS             7846..8811
                     /locus_tag="IYA83_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587567.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="IYA83_002151"
     CDS             8798..10120
                     /locus_tag="IYA83_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ8587568.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="IYA83_002152"
     CDS             10361..11104
                     /locus_tag="IYA83_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587569.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="IYA83_002153"
     CDS             11119..12111
                     /locus_tag="IYA83_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ8587570.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="IYA83_002154"
     CDS             12123..12860
                     /locus_tag="IYA83_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587571.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="IYA83_002155"
     CDS             12850..13572
                     /locus_tag="IYA83_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ8587572.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         4624 a         2298 c         2808 g         3916 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgaggtc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaat
//
LOCUS       DACIFS010000068        13600 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.068, whole genome shotgun sequence.
ACCESSION   DACIFS010000068 DACIFS010000000
VERSION     DACIFS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.068"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(115..1245)
                     /locus_tag="IYA83_002156"
     CDS             complement(115..1245)
                     /locus_tag="IYA83_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ8587573.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1786..1992)
                     /locus_tag="IYA83_002157"
     CDS             complement(1786..1992)
                     /locus_tag="IYA83_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587574.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2008..2622)
                     /locus_tag="IYA83_002158"
     CDS             complement(2008..2622)
                     /locus_tag="IYA83_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ8587575.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2769..3452)
                     /locus_tag="IYA83_002159"
     CDS             complement(2769..3452)
                     /locus_tag="IYA83_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ8587576.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3561..4019)
                     /locus_tag="IYA83_002160"
     CDS             complement(3561..4019)
                     /locus_tag="IYA83_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ8587577.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4103..5866)
                     /locus_tag="IYA83_002161"
     CDS             complement(4103..5866)
                     /locus_tag="IYA83_002161"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ8587578.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6087..7547)
                     /locus_tag="IYA83_002162"
     CDS             complement(6087..7547)
                     /locus_tag="IYA83_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587579.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7755..8681)
                     /gene="glsA"
                     /locus_tag="IYA83_002163"
     CDS             complement(7755..8681)
                     /gene="glsA"
                     /locus_tag="IYA83_002163"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ8587580.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8704..10131)
                     /locus_tag="IYA83_002164"
     CDS             complement(8704..10131)
                     /locus_tag="IYA83_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8587581.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10162..11376)
                     /locus_tag="IYA83_002165"
     CDS             complement(10162..11376)
                     /locus_tag="IYA83_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ8587582.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(11717..11959)
                     /locus_tag="IYA83_002166"
     CDS             complement(11717..11959)
                     /locus_tag="IYA83_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ8587583.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11991..12548)
                     /locus_tag="IYA83_002167"
     CDS             complement(11991..12548)
                     /locus_tag="IYA83_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ8587584.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12561..12749)
                     /locus_tag="IYA83_002168"
     CDS             complement(12561..12749)
                     /locus_tag="IYA83_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ8587585.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12762..13328)
                     /gene="amaP"
                     /locus_tag="IYA83_002169"
     CDS             complement(12762..13328)
                     /gene="amaP"
                     /locus_tag="IYA83_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ8587586.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4066 a         2810 c         2221 g         4503 t
ORIGIN      
        1 atgatttttt gagatacttt cattgcgaat tcctctttct aaaagaacct ctcgcttgaa
       61 aaataaaaga ttgtacttta tgataattca aactgagagt ttctcagttt ctctttattg
      121 tttttcaggt cgatagagcg ccaactcttc ggcctgtatc tttgaagaaa tattcaacgt
      181 gattccgatt cctaaagaaa ggatcagata acttgttcct ccgtagctaa caaatggcaa
      241 tggcacacca gtcattggca tcagccccgc gatacttccc acgttcatta tcgtctgaac
      301 gaatagcaaa gttccgaatc ctaaaagaaa caatccagct tgctgatttt tacagcgact
      361 gcttaaacaa aagattctca tgcataatga gaacaataag aaaagcacac acaatgcacc
      421 gattagccca agctcttctg taataatcga aaaaataaaa tctgtttctg tctctggaag
      481 ataccctttt tttgtgatac tatttcctaa tccccgccca aatatgccac cattatatag
      541 tgcataaaac gaatgggtca tttgatagcc cgcaccatga ctatcgataa aagaatctct
      601 taaagtcgcg atcctttcat aagcatgatt aaacatctgt ggaagccaac ctttatttcc
      661 taaatataat accccgcctg ctgctccaat cagcaatgca gaaaaagtaa cgattagata
      721 gatccctttt ttaaatggaa ccgccgctgc ccaaaatatg acccacgcaa tcgaaaggat
      781 catcaatgct ccagcaatct ttggctgaaa taaaatcaga aaagttatac ctactgtaat
      841 caggaatggt ttttttagat cttttgggct actatttccg tcacgaaaaa accagcttaa
      901 ataaaaaatc aaaaaaagat ttgccagttc agaaggctgg aattgaatgc caaacagaga
      961 gatccaacgt tgtgcgccgt tgacagtgac accgaatatc cctattctta ctaatactaa
     1021 aaagaaaatc gataaggcta aaccgtatcc tgctatttta gggtgaagta aatagtgtag
     1081 tttgactgaa cgagctaaga agatcacgct ccaactcaaa aatatgaaaa ttagttgacg
     1141 caataataga ctttttgtat tttcatcggc ttgcaataaa cgataagaac tggcactgta
     1201 tacttctaaa agaccgatca tggacaaagt caaataagga cccaaaatcc accaatcgat
     1261 cttttttcac cgcatctcct cctcacacag caatccactt gtcaggtgag gagcgagttt
     1321 gtctcatcaa ataagtcagt cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca
     1381 agaattctga cctagcatca tctcatcgtc cgagttttag cactatacaa cgtaaagaaa
     1441 acattcttag ctattggaaa aagccttaat ccaacagttt ctgttttacc cattggcgac
     1501 tttcgacgtt tctgggcaac agcctatgtt tgtataggag cctcacctaa caaggttatt
     1561 actatattct atttacgagt tcatgataga cttttcctaa tgattaggca atggaatttg
     1621 aaatgaaata gtgaaattta acaatcttta atatttttta gaaaaaaata acaaataagt
     1681 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttagt
     1741 tttatgtgtt tttttatatg ttttttcact tttcggaata aatatttatt tgttaaagta
     1801 attaaacaat ccgctaatcg tttgatctgg catttctatt tccttaccat ctaaagtaac
     1861 agtagtaacg acaaaatggt cttgcaatgg cggaacttct aagtactgcc cttgcagaaa
     1921 atcattcact gtttcatatt cttttttcat ttcttttgaa tctgtcgatg gtttatagct
     1981 tatttttaac ataactagtg ctccttttta ttcgggacgt gcaatcgtga aaatttctcc
     2041 tattgctgca taatcatttc ctgccaaacg gcttacagca ttcaattttc ttggatcgat
     2101 ccttcctgtc ttatcatcat agactgtttc atttaaatga tatttctgta cttctagcaa
     2161 aaataaatca gctgttactt tatttttatt ccgtatctcg acatgttcat ataaggatgt
     2221 ttcaaaacga acttttgctt ctgtcaatgc tggtactgaa atgattactg aatcagttaa
     2281 agtcaagtca gtcaggtaca gttcgctttc ctcattaggc agattggctg ctgttttatt
     2341 tacttcttct acgttgtcct catcaaggat atggataaca gcttctcttg tctcaagaat
     2401 attctttgct gtgtctttta ctgcatcttg ttttctctgg atcgccaagg ataagatggg
     2461 tcgattcgaa ctgacgatat tgaagtaact gaagggtgcg atattcacta tatcttttgc
     2521 tgatttagtc gttactactg ctactggtct agggatgact gaaccaatca gcaacttgta
     2581 attttcacgt tctgtcaatt ctgaaggact ccgttcaaac atggtttcgc cttctttcta
     2641 taaaattcgt taaaaagcag atcttgaaga caaaatgaaa agtaatgctt ttttcatctt
     2701 cttagaatct gctttaaact atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg
     2761 cgtgtttttt agttatttgc tttacttgcg gcttcgacat atcttttagt gatcaattgc
     2821 ggacgggtga taatagatcc aactacgact gaaaaagcac ctaattcata tgctttctct
     2881 acttgttcag gagagtcagt atgtccttcc ataattactg ggatatcgac ttgttctatg
     2941 atttgattca ataaatcaaa atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct
     3001 gctaaagtcg tagacaaaag atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa
     3061 gtcgaaatat ctgccatagc taaccgaccg tgttcgtgaa tcatttggac aagttcagca
     3121 gttgtttttt ctgtcggacg tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc
     3181 gtctccaaaa gttcttccac ttctttttct gtcgcagtaa taaacacttc tgacccatcg
     3241 taatctcttt tgataatccc tattaccggt aaatcaacga ttcgcttgat ttcattgata
     3301 tcttctgtcg tatttgctcg aataccaact gctccgcctt cttttgcagc caaagccatt
     3361 ctccccataa taaaagaact gtgtagcggc tcgttttcca aagcttgaca agaaacgacg
     3421 actccgcctt tgatttgttc cagtgtatcc acaatatctc ctccagtaat gttatataaa
     3481 aatacagctt catataaaaa tgtaaccctt ttctattcag gagacaataa tgagctattt
     3541 ctttcagcag acctttctta ctaaaagaaa attacttcaa attcttcgta tacgaccagc
     3601 ttctcttctg taaattctat gttcaacgct tctttataag catgtttaaa aaactcagca
     3661 tggactttcc gccaataagc taatgtccga tccccttctc cttctttcca agcatgctct
     3721 ttcgtgactt cactaaaagg caagatggtc acatttatat ttttgatgat acaaactggt
     3781 tcattttttc cattcaggac cacgtctaat tgaccaactt ttggtaaagg ctcattctct
     3841 tgtttataca gatcgtaacc acttgatgta ccagtcttta tcttttttac aactagttcg
     3901 ctcaactcgt ctgccatttt ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat
     3961 tgataactag gatatttttc ttggaacttt ttgacatatt cttctacttg ctgatacata
     4021 acatccaccc ctttgctttt tataatgaaa aaaccatcca gatactagta taaacaatta
     4081 tctggatggt gagcataatg aattattgaa tattcaagac acgcagacta tcttcaacga
     4141 tttgttttgg tgttaaatga taacgttcat aaagctgccc tacaggaacg ctatccgtga
     4201 attctttctc tgcaccaaaa tttaatacgt tcattgaaga atcaccataa taacgactga
     4261 tattttcgcc aaatcctcct gacaaactac catcttctaa tgtgatgact aattcgtgtg
     4321 tttcttttaa tttatctaat gtttctacgt cattttctgt gatcgttcct ggattgatca
     4381 acgttgcttc gattcctttt tttctaaaag cttttactgt ttcttttcct aattcaagaa
     4441 aaccgcctaa agctaaaatt gctacttttc ctccattatt tactgtttga taggctggaa
     4501 tactataatc atttgcgata gctgtaccat gttctacacc atgtgtagga attcggattg
     4561 ctacaggctg cgctgtttgt tcgaacgccc aatctagcat agagatcata tcctcttttg
     4621 ttgctggagc aagatatttg atgttcggga tacttgataa gtaaggaatg tcaaatgttc
     4681 cttggtgtgt tcggtcacta gctgagatcg ttccgccacc gactaaaata atcactggat
     4741 tctcgttgat cgctaaatca tgtgataatt gatcatatgc ccgttgcaag aaagtacttg
     4801 attggaagac gactggacgt actccctgag aagccaaagc agaagcgaaa gtgatagagt
     4861 gctgttcagc gatccccacg tcaaaataat gatctggatg tttttcttgg aatgttttca
     4921 gtccaaaggc gcctggtatc gctgcattga ctgcggcaat aggagctgta cttgtttgca
     4981 ttttcttatc caaataatcc atgatgatca tattaaatgt ttctccagaa gttacttgct
     5041 ttgcctcccc ggtggacaga tcaaatggta catgccaatg atatttcatt ttattatcga
     5101 ttgccggctg ataaccgcga cctttttccg tatggacgtg aagtacaatc ggatgatcaa
     5161 tatcttttac ttcttcaaat aaacggatca ccgtttccag atcgtttcca ttatccacgt
     5221 aacgataatc aaatcccatt gctttaaata ggttattgct gcttttaccg ttggtttgac
     5281 gaagctcagc taagttttta tataaactgc catgattctc atcaatagac atctcattat
     5341 cgtttactaa tacgattaga ttggattgta aaacaccacc attgttcaat ccttcaaaag
     5401 ctaagccacc acttagggag ccgtcaccga taaatgctac cacatttcct gattctttct
     5461 tcagatctct cgctttagct aaacctgttg ctaagctgat agatgtagat gtgtgcccca
     5521 ctgtaaaata gtcatgttta ctttcatgtt ggttggaata tccgctcact tcacggtaat
     5581 gtcctttttc aaaaccattt tttcgaccag ttaaaagctt atgaccataa gactgatggg
     5641 aaacatccca aacgatttta tcctttggtg aatcaaatac ataatgaaac gcaatcgtta
     5701 attctactac acctagattc ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga
     5761 ttttttggcg catctcttct gccaactgtt tcatttcctc aacagataat tttttgacat
     5821 ctgcggggct attgattgca tccaatacat gtaatactgt actcataata cattctctcc
     5881 tccgtactat cttcttttaa tgaaaataga tttgttacac tttatctatt ctactactta
     5941 gagttgactc taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt
     6001 agatcttcgc acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg
     6061 tagaaggtct cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc
     6121 tgagcaatat gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc
     6181 ttttcagttt ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc
     6241 gcacatagac attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca
     6301 gcgatgcctt cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa
     6361 ctttctgaaa caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa
     6421 gaaagacttt tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt
     6481 ttctttttcc actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct
     6541 gatagaagat agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca
     6601 aattgtaaaa aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta
     6661 ataatctgta aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca
     6721 acttcgaaat cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac
     6781 aaagaaaaaa tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc
     6841 aatccttgac agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct
     6901 ttcagtccaa ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag
     6961 gacttttcat cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac
     7021 tcgttacaag cttcttctac atacggattt ttattttcat agccttccca attgacataa
     7081 cgtactaaag ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt
     7141 ttcaaataat cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg
     7201 ctcaaagaaa taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa
     7261 taacttaaaa actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga
     7321 gcaataccat gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata
     7381 caatattcta gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc
     7441 cttagagaaa tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac
     7501 agatacactt ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc
     7561 ctccagtttt tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt
     7621 ttatttagaa aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca
     7681 agctcacaat ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc
     7741 tctttcaaga atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac
     7801 aattggatac cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg
     7861 ccgtaacgat ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca
     7921 ccaacatgga cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat
     7981 ttgacgattg tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct
     8041 ttatttgcca gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat
     8101 tgtctgaaat aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc
     8161 atatagtaag caagagagcg attgatatca cctgttcttg attcagaatg atagatttct
     8221 tcattcaatg tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca
     8281 aatttttcat ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta
     8341 acaaaaggat tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag
     8401 gcaaaaccgg ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg
     8461 attgctaata acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg
     8521 tctcctccct caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg
     8581 ggatccactt cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg
     8641 aaattttttc caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat
     8701 tttttatttt tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac
     8761 taagacgata agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc
     8821 gatactagca ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa
     8881 acaagcaacg attactttta caacttttcc gcctggaacc tgataggctc tttcaagact
     8941 gtcatcttta aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta
     9001 gattacaaca gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa
     9061 tgtaaagacg gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg
     9121 aacaccgtgt gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac
     9181 gtgaagtgct tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca
     9241 aacaagaatc gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag
     9301 gaattctaac gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt
     9361 agcgacggtc aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat
     9421 tgctaatgga taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc
     9481 tacgcccata tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa
     9541 atcaggaatc aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata
     9601 atggatccct aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat
     9661 ccgagcaatc aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa
     9721 aataatcaat aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt
     9781 tgcataggat aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc
     9841 aaaccattgg aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca
     9901 agtatagaca ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc
     9961 cacagggata aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga
    10021 ggtcgcgaat gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa
    10081 gccaaagaac gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc
    10141 tcttttctat ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc
    10201 ttcttcttgt tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat
    10261 cactgttgtc agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc
    10321 taagaagtac cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat
    10381 cagaatcgta cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc
    10441 ccattgtttc tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc
    10501 aatcagcatg gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc
    10561 agcaacggat cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat
    10621 gatcagccaa gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac
    10681 tgcttgttca tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag
    10741 cagtcctgta ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg
    10801 aatctttctt tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg
    10861 gatgactgct ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca
    10921 tatccagtgg caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata
    10981 gccttttaaa ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat
    11041 aaacatcagt tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt
    11101 ggggaagctt gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc
    11161 gatatcccgt ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc
    11221 aacggagaga aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc
    11281 gtaaaaaaaa gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa
    11341 tgctgtatct cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt
    11401 acgagtataa aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca
    11461 tttataaaag acagacaatt aataaaagct tgatgtgttg tgctcttttt cgtttttcac
    11521 acaaataaag acaaaaaata aacggtcttc tttttataga aaaccgttta ttttcgtctt
    11581 tttttatttt taaagatatt ttttcataag gaataaagaa tacttcatat aaaatatata
    11641 aaaaagaacc gcagcgagtg tcgctacggt tctcaaattt gttcttcatt tcaacataag
    11701 tttttcttca atttgcttat aatttacgtg caaagaatga aacgactaat actaaaatga
    11761 ttgcaccgat caatgatgga atgatagcca ttcctgcaat tgttggtccc caagttccta
    11821 gtaaagcttg tccaattgct gaacctacta aaccagcaat gatgtttgcg atgatcccca
    11881 ttgatgatcc tttattcgtg attgctcctg caattgctcc aatgataccg ccgacgatta
    11941 atgcccaaat aaatcccatt ttaaattcct cctaactgac ttcttacttc ttattgtaca
    12001 cgtggttcgt tattttcttt gactttttgt gcacctttgc ctacagcaga ttttgcttta
    12061 tctgtttggc gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac tttgtcttgt
    12121 actgtttctt gatctttttg gtattcttct tgtgttttga tgtctgcaac attcacattc
    12181 acttcgataa catctaaatg tgtcatattg tttacttcag tactgatcaa ttctttgatt
    12241 tggttgtaaa tatcttcgat gtctttaccg tattctacga tcacatctag atccacagct
    12301 acttgtttct tacctacttc tgtattgata cctgctgtta cgttatctgt gttgactaat
    12361 ttttcagcca tatttgagaa aaatccgcca tctaatgtca atagaccatc tacatctttc
    12421 aatgcataac cgatgatttt ttggatgact ttgtcttcat aagtcaattc accattcatc
    12481 atgcggtttt ctcctttttc atttgccaaa actgatccat ttgttgtatt catgtttttg
    12541 ttttccatct ttgtttcctc ctatgattta aaaaattgat caactattcc tgactgtttc
    12601 aaataaaaac cgattcctgc gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa
    12661 aatccaatcg ttaaccataa aacagctagc agtagtccaa gacccccaaa gataataggg
    12721 agcttgtatg ctgaaaatag ttctttcatt atttggccct cctattccac tcgagagtgc
    12781 ttgtagtctc ttgtatcttc ttgttcttca taaccgctgt atgtgactgc cactttgacc
    12841 gtttcttttg ttccgagtac tttagttact tcttcttcga tatcttgcat caatacacct
    12901 gttttcccta ctaatgaaga agttcgtttc aagtctccgt tgacatggat atggattcga
    12961 tttttcgttg aacgtatccg tactttaggt gaatgaatga actcttcttc gtgtaaatgg
    13021 gaacgtacca acccttcgat cgcttttttg tcgattgtta gtttgccatc ttcccgtttc
    13081 ataacgaaag ttcctcttga ctttggatag aaaagaacga ctagcaagat gataatagca
    13141 agaacggcaa atactacagc tgcccagaaa atatattgtt gcatgtagta attcgttaaa
    13201 gtaaagaagc gaaatcgttc caattgttca ggcataatat acggcgcatt gatcaacgca
    13261 atcgctagaa atactgaaag tagcagtaaa ctcatgataa caccgattat ttttaacccc
    13321 ttattcattt tccacctcct ataaacttat ttttaccctg taaataactc taccgacaat
    13381 cacagtatag atagcgaaca gttagcatag catcagtaaa taaagcgcta ccaaacattg
    13441 ctggatcata acggcgtttt attgccagtc tgatactgaa ataacaattg cttacttatt
    13501 ctctatttac ttttgattgc tggctatact taaaggatat tattcaagat taaaaattgc
    13561 aaagattatg ctcagttatt attacaagat tctatttatt
//
LOCUS       DACIFS010000069        13147 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.069, whole genome shotgun sequence.
ACCESSION   DACIFS010000069 DACIFS010000000
VERSION     DACIFS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.069"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(77..149)
                     /locus_tag="IYA83_002170"
     tRNA            complement(77..149)
                     /locus_tag="IYA83_002170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IYA83_002171"
     CDS             530..1099
                     /locus_tag="IYA83_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ8587587.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IYA83_002172"
     CDS             1086..1946
                     /locus_tag="IYA83_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ8587588.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IYA83_002173"
     CDS             2136..2840
                     /locus_tag="IYA83_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8587589.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IYA83_002174"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IYA83_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ8587590.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IYA83_002175"
     CDS             4677..5993
                     /locus_tag="IYA83_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587591.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IYA83_002176"
     CDS             5994..6863
                     /locus_tag="IYA83_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587592.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IYA83_002177"
     CDS             6918..7727
                     /locus_tag="IYA83_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ8587593.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IYA83_002178"
     CDS             complement(7830..8459)
                     /locus_tag="IYA83_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ8587594.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IYA83_002179"
     CDS             8629..9921
                     /locus_tag="IYA83_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8587595.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IYA83_002180"
     CDS             complement(9997..10659)
                     /locus_tag="IYA83_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ8587596.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IYA83_002181"
     CDS             10884..11168
                     /locus_tag="IYA83_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ8587597.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IYA83_002182"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IYA83_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ8587598.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACIFS010000070        13085 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.070, whole genome shotgun sequence.
ACCESSION   DACIFS010000070 DACIFS010000000
VERSION     DACIFS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.070"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(79..2199)
                     /locus_tag="IYA83_002183"
     CDS             complement(79..2199)
                     /locus_tag="IYA83_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ8587599.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="IYA83_002184"
     CDS             2429..3607
                     /locus_tag="IYA83_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ8587600.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="IYA83_002185"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="IYA83_002185"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ8587601.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="IYA83_002186"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="IYA83_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ8587602.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="IYA83_002187"
     CDS             complement(4960..6213)
                     /locus_tag="IYA83_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ8587603.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="IYA83_002188"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="IYA83_002188"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ8587604.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="IYA83_002189"
     CDS             7790..9136
                     /locus_tag="IYA83_002189"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ8587605.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="IYA83_002190"
     CDS             complement(9444..9830)
                     /locus_tag="IYA83_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ8587606.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            10709..10921
                     /locus_tag="IYA83_002191"
     CDS             10709..10921
                     /locus_tag="IYA83_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587607.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="IYA83_002192"
     CDS             complement(11199..12998)
                     /locus_tag="IYA83_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ8587608.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DACIFS010000071        12771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.071, whole genome shotgun sequence.
ACCESSION   DACIFS010000071 DACIFS010000000
VERSION     DACIFS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.071"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            198..2570
                     /locus_tag="IYA83_002193"
     CDS             198..2570
                     /locus_tag="IYA83_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587609.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2631..4091
                     /locus_tag="IYA83_002194"
     CDS             2631..4091
                     /locus_tag="IYA83_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ8587610.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            4102..4545
                     /locus_tag="IYA83_002195"
     CDS             4102..4545
                     /locus_tag="IYA83_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAQ8587611.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQ"
     gene            4630..6306
                     /locus_tag="IYA83_002196"
     CDS             4630..6306
                     /locus_tag="IYA83_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAQ8587612.1"
                     /translation="MNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQ
                     KEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVETE
                     KKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAIQGLYEGKYLSYPRTDTPYITEGEY
                     AYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLS
                     PLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPLDLGWKKLFGKES
                     DGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEA
                     QSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSA
                     EMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAAS
                     EKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQN
                     SRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTAK"
     gene            6552..7148
                     /locus_tag="IYA83_002197"
     CDS             6552..7148
                     /locus_tag="IYA83_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8587613.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            7161..8060
                     /locus_tag="IYA83_002198"
     CDS             7161..8060
                     /locus_tag="IYA83_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ8587614.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            8063..8547
                     /gene="ssb"
                     /locus_tag="IYA83_002199"
                     /pseudo
     CDS             8063..8547
                     /gene="ssb"
                     /locus_tag="IYA83_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8569..8826
                     /locus_tag="IYA83_002200"
     CDS             8569..8826
                     /locus_tag="IYA83_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587615.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8985..9119)
                     /locus_tag="IYA83_002201"
     CDS             complement(8985..9119)
                     /locus_tag="IYA83_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ8587616.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            9262..9522
                     /locus_tag="IYA83_002202"
     CDS             9262..9522
                     /locus_tag="IYA83_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587617.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9971..10177
                     /locus_tag="IYA83_002203"
     CDS             9971..10177
                     /locus_tag="IYA83_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ8587618.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            10177..10428
                     /locus_tag="IYA83_002204"
     CDS             10177..10428
                     /locus_tag="IYA83_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587619.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10444..10881
                     /locus_tag="IYA83_002205"
     CDS             10444..10881
                     /locus_tag="IYA83_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587620.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            11317..11505
                     /locus_tag="IYA83_002206"
     CDS             11317..11505
                     /locus_tag="IYA83_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587621.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            11563..11829
                     /locus_tag="IYA83_002207"
     CDS             11563..11829
                     /locus_tag="IYA83_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587622.1"
                     /translation="MGTGFIDRVLVQVKEERKITMKYNLLDHKRMWVEKLEEDLEQLE
                     LLGHNKTSTVYKEGLKRTKKAQKELATLESRVQKKEVDLTHTYL"
     gene            11839..12072
                     /locus_tag="IYA83_002208"
     CDS             11839..12072
                     /locus_tag="IYA83_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HAQ8587623.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12415..12597
                     /locus_tag="IYA83_002209"
     CDS             12415..12597
                     /locus_tag="IYA83_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ8587624.1"
                     /translation="MSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSEIVDRGVK
                     AYVNGFTKVLGGVGGR"
     gene            12587..>12771
                     /locus_tag="IYA83_002210"
     CDS             12587..>12771
                     /locus_tag="IYA83_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587625.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         4732 a         2108 c         2677 g         3254 t
ORIGIN      
        1 aacagggtag acaaacgcta caggaggaaa tagcaaattc ccgttggatc ctttccatac
       61 atccattgta ctctgagaaa tcacacaata tcgaaacaaa ttccgttagg aatgtgttat
      121 tcaataaatt ttataatcta gaaacatttt gaataaaaac tattagacaa atgatatttt
      181 acgaggagga aacggacatg gcagaagaaa aagaaccact agacaatagt cgtttgggaa
      241 aatccaaaag aaaattagta cgattgcaaa atgaattaaa tgatcaaatc gaaaagatgt
      301 ttgaacacca aagaaagacc aacggtcaac caatgaacga taagagaaac gggcattctt
      361 ggtttaggca acaagaacga atcgaaaata aagtccaaag tctgagagaa gagatcaagc
      421 aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa atgggttata
      481 acaagtacgg tggcctggat atgacgatcg aaaatattcc aagaatcaaa gaagaaatcg
      541 aacgctttga aaaaggtgaa tcgacttttt cagctgccac gatccgaaaa taccaacgaa
      601 aattggaaac attagaacag ctgaaagaaa aatcagagaa aggaaaagaa aatattcttc
      661 ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaag aatccaacta
      721 tctattttct gaaaggctat cgaaaagtcg ctttagaact caatgaaaac gggggatttg
      781 aggaatctaa aaaataccaa gccacgtcgg aagaagaaaa ggcagctgtt cgtgaattac
      841 ttgaagaagc tacacctact gattctgtga aggagaaaga aaccgatcat ttctatcgag
      901 tagaattcaa cgaaaatgat ccagcaaaag ggttgaaaag ttatggtggg gagatcgtaa
      961 cactagaatt gatcgatcaa ctcaaaacac tggataacca atacgccgca gaagcaggct
     1021 attataaatt ctattttgaa gaagtgaaga atggagaagt agtagatcag atacgaatgg
     1081 atctagggga tggcgtcaat agtaatcacc ccatctattt agagttagct agattgtgcg
     1141 aacaagtacc tgagaaggaa caaaaacaaa cagtcaaaaa tgaacctatt tcaaaagtac
     1201 aaaatgagat taaagagaca aaaacaaaac aaaagggtta tcaaaaacga acggcagaag
     1261 agattaagca agaagtcaaa tcactttccc aacaggcgct tgatgcggtg aaaaagcata
     1321 ctcattcacc tgaagacgtc aaagaacttt tgaattttat gtctcgtttt ccagaacgat
     1381 cctttagaaa tcaattattg atcgaggaac aatttccagg cgcaacggct tgtttaggtc
     1441 gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg aacaagatct
     1501 ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt cgagaaacag
     1561 aggctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg atcaaaaaac
     1621 cttactacac gattgaaaaa gtgtttgata tcacacaaac acaattgaag cctgaagact
     1681 atccgaaaat cttcccgaac cgcgtctttg attttcaatt ggatcaaaca gggcatacag
     1741 aacttcaagt tgggattcaa gcggtagcca aaaatattgg aattcaaatt cgagatatga
     1801 cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtac gttcgtaacg
     1861 aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg cagcatttag
     1921 ccacaagtat tcacgaatta gcccatgcta gaatgcacaa attttcagaa ttagatactg
     1981 caacaaaaga actacaggca gaaatgacct cctatattgt gtgcaagcat tttgggatgg
     2041 acacctcgga gaaagctatt ccttatattg cgacatggac gaagaatggt cagacgttgg
     2101 atgataagga agctgcggaa cgtgggaaaa tcatgaatga tgtcagtcgt gtcgcaaatg
     2161 aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa ccagagatcc
     2221 agccagcaga agtgaagcag gatgagaaaa atgttgtaga aacagcagaa caaaagaata
     2281 ttgtgctcgt tggctcactt tggatcgaca agaaagacgg gaaagtcctg gaagaagaca
     2341 cgggccgaac gttacatttg aaggatgcgg cgtttattga agatacgcag cataatcaag
     2401 tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct tcagaagaat
     2461 ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg gccaaacgaa
     2521 aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa aacaagtaaa
     2581 aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa atgaccgaga
     2641 aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt gctcgaaaca
     2701 atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat gattcatttg
     2761 tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt ggcgacatta
     2821 ttgaattagc taaacttttt ttcatagaca aagagattca agactctaag caacaattta
     2881 aagcagccac agattttatt ctaaagaatg aagacaaaac agaacgagta gaaaatcttc
     2941 attttgagac agaaaagtac aaagatcacc ccgttgatta tcaaccactc acagaaaaag
     3001 gaaggaatta tttgaaggaa gaacgaaaac tgcccgattg gttgattgat tatgcagaaa
     3061 aagaaggatt gatcgccgaa ctaaagccaa aacatgaacg gcaaaatttc ttagttcaag
     3121 acgatcgttt ggatcatgcg gtcgcctttt tatggaaaga tccacagaca aaagagacag
     3181 taggggcgag ttatcaagga acattcattg attatgaacg ttttggtgag agaggcacat
     3241 ataagcatat agataaaaat tcgacagcga atcatggatt taacctaaag atcggtgacc
     3301 ctaaacagtt aaaatttttt gagagtagta ttgatttact tagctatgct gcactgaatc
     3361 gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac catgtgatca
     3421 gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt cctcaatcca
     3481 ttgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa gaacaattaa
     3541 tgggcgcagt tgatccattt acgaatcaga aagtacgttg tgaacgagga atcgctaatg
     3601 attggcaagt gccaaaagaa tacaaagtca tttatgaaga agttgcaaaa gaaatgaaag
     3661 ttgaacctga agcaattatg gccatacaca agacagaaaa caatctgcaa ttaacgaacc
     3721 aactggtttc cgctcacaaa gtaaatgcgt cttttggtca gcagctttct gttaacgatt
     3781 caatagaagc tattaatttg aaagatattt gtagagaagt cgcaaaagaa ttaaaaggtt
     3841 gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa ggggatatta
     3901 atgctcagat tcttttctct tataaagcag aacaatacta caaaggctat aaaaatcatg
     3961 aacatgaatt tgtacctgaa gtaaaaaagg attggaatga tcaattgaag catgaaatcc
     4021 accaacaaga gatcagaaag caaaaacgtg cgatgttgtt tcaacaaggc aaacaacaag
     4081 aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc gtcacaagcg
     4141 aaagcttatg ccgatagctt ctctaaagcc acccgaaaag acgggtattt tgaaatccaa
     4201 gatagattgt ttcctggtga aactgtgatc acgtatggtt ttggtcattt agtcgagtta
     4261 gattcgcccg atatgtacga tgaaaattgg aaacaatggt cattagagca tttgcctatt
     4321 ttccctacac attatcacta tcatgttcct aaagataaga aaaagcaatt caaggtggtg
     4381 aaacagcaac ttcaatcggc cgacaccatt attattgcga cagatagcga tcgtgaaggg
     4441 gaactgatag cttggacgat cattcaacaa gcaggcgccg atcatggaaa gatatttaaa
     4501 agactttgga tcaactcctt agaaaaagaa gccatttatc agtgattcca gcaattacga
     4561 gacgcagaag agacctatcc caaatttgaa gaagcacaag cacgccagat cgctgattgg
     4621 ttgatcggca tgaacggcag cccactgtat agtttactct tgcagcaaaa aggcatacca
     4681 ggaagttttt ccttaggcag agtccaaacc ccaacgcttt acatgattta tcagctgcag
     4741 gaaaagatta gaaacttcca aaaagagcct tattttgaag gaaaagccca agtaatcgct
     4801 caaaatggcg catttgatgc gaagcttgat ccgaatgaaa cccaagcgac gcaagaagca
     4861 tttgaagatt atttaaaaga aaagggggtt caattgggaa aacagccagg tacgatccac
     4921 caagttgaaa ccgagaaaaa aagtgcggca agtccacgtc ttttttctct atccagcctt
     4981 cagtctaaaa tgaatcagct catgaaagct agtgccaaag atactttgga agccatacaa
     5041 gggttgtatg aagggaaata tctaagctac ccacgaacag atacccctta tattacagaa
     5101 ggggagtatg cgtatctgtt ggatcactta gacgaataca aacacttttt aaaggccgaa
     5161 gccattccta ctcctattca tacaccgaat agtcgatatg tgaataataa gaaagtccag
     5221 gaacattacg cgatcattcc tacaaaaacg gtcatgacgg cagcggcttt tgaacaatta
     5281 agtccactgc agcaggccat ttatgaacaa gtgttaaaaa caacggtggc catgtttgcg
     5341 gaaaaatata cctatgaaga aacaacgatc ttgacgcaag ttcagcagct tcagttaaag
     5401 gcaatcggaa aggtgccgct agatctaggt tggaagaagt tatttggaaa agagagcgac
     5461 ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga aatggtgacg
     5521 gtggatctac aagttcttga aaaagaaacc aagccaccac aaccttatac cgaaggtacg
     5581 ttgatcactg caatgaaaac agctgggaaa accgtggata gtgaagaagc gcaatccata
     5641 ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat tgaaaccttg
     5701 aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa taaagggatt
     5761 ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gcgccgaaat gaccgccaaa
     5821 tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac ttttctagcc
     5881 aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat ccaatccact
     5941 gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga agccgcgcgt
     6001 catttaggaa tttgtccaaa atgccaggaa caagaagtct tgctctatca aaaggtggca
     6061 gcttgtacgt ctgaagcctg tgattttaaa ttatggacaa cgattgccaa aaagaaactc
     6121 acagcgacac agctgaagga gatcattcaa aatagccgaa caagccaacc agtgaaaggg
     6181 ttaaaaggtc agaagggttc atttgaagca acgattgttt taaaagaaga cttcactaca
     6241 ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg ccgcactgca
     6301 aaataatgga gggttaatct tatgcgtaat accacacaca aacaacaaga aatcgaaaat
     6361 caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg aagactgcag
     6421 caattgaaga accagcagag gattcaaaaa gaaatcgaat tagatcgata aaatcttcct
     6481 aactattgta tacgtataaa cttatgttat actgttttta taagtttata cgtatacaaa
     6541 ggagggagaa aatgacaaaa gcaatggtat acaagctgct agaagaattt aatggaactt
     6601 tggctttgaa ggatgccaaa aaagcgggca tttctcctgt aacgatcaaa cgtatggtag
     6661 atcgagggga gctggatcga gagtatccag gttttttcac cttacctggt caatttcctg
     6721 atgaattgtt tatggcgcaa aaaaaatatg aacgcggcat tatttcacat attaccgcgt
     6781 tagatttgta tgatttaaca gatatgatcc ctagacagat cgatctgact gtaccttacg
     6841 gttatcacgt ttctgaaaaa ggattgaagg aattcgcagt tgagctacac tatacaaaag
     6901 cggaatggta tgaattaggt aagattgaaa taaaaagtcg ctatgggaat cctattatag
     6961 cttatgatac agaaagaact ttatgtgaca tttggaatcc ctggtatacc gttgaggatg
     7021 aaatcaaagt aaaagcaatc aaaaactata tggagtcgga cagaaaaaat cttagaaaat
     7081 tgaatgagta tcgacgaatt ttaccaacag acaaaacgat gcgttcttat attatggctt
     7141 taaattaaag gagaagatac atgacaccta cgcagttaaa agcaaaagtg aagaatattt
     7201 ctagagaaaa agaagttgat ccgcagcttg ttttaagaca ttttatgatg gaaaaattct
     7261 tggaaaaaat ctcagagtct ccttatcgtg aaaattttgt gttaaaaggc ggcttcttga
     7321 ttggctcaaa gtatggcatt gaaaatcgca caaccaaaga tattgatacg actttacggg
     7381 aaatgaaagt aaccaaagaa acattaacaa cggttctaaa cgaaattttt tcaacgccga
     7441 cgaaagaagg cattcaattt gagattcaag gaatgaaaga aacgagagaa gccgattact
     7501 atccaggatt tagcttacgt gtgctggcac atttggaaaa catgcggcca gattttaagg
     7561 tagatgtgac tacaggtgac tcgatctatc ctgcaacgat cactcacagt cataaattga
     7621 tgtttgagga tcgaacgatt cctttggaat cctatcctac agaacaaatt attgctgaaa
     7681 aattaagtgc aacctttgat tttttaacag ataattcgag aggaaaagat ttttatgatc
     7741 tctatactat tcctaaaatg gagaagatcg atgagaagac cttgaaagat agtgtgagaa
     7801 atacatttac acgtcgagga aaaactgatc cgttaaagat atactatgaa aaggatatgg
     7861 atgtactgaa aaatagtcac gagctaaaag atacctggaa gaaatatcaa aaaagcaatc
     7921 cttatgctgc atcgatcacg tacgatgaga caatggattc gattagtaat ttgatggaaa
     7981 aagtgattca cgtcgaagaa aaagagcaaa aactagctta tcaaagacac caacagatga
     8041 atagagaaat ggagagataa agatgattaa taatgtgaca ttagtaggac gcttaaccaa
     8101 agatcctgat ttacgttata ccgcaagtgg tactgcagta gcgactttta ccctagccgt
     8161 caaccgaaat ttcacgaatc aaaacggcaa tcgagaagcc gattttatta actgcgtgat
     8221 ttggcgcaaa ccagcagaaa caatggcgac attagctaaa aaaggtagct tgattggtgt
     8281 agttgggcgg attcaaacgc ggacttacga caaccagcaa ggacaacgtg tatatgtgac
     8341 tgaagttgta gcagataact ttcaactatt agaaaataaa gcagcgacag agagccgtgc
     8401 gcacgctgat caatcgtcca ccagtccaag caccgccacc tttgaacaac ggatacagcg
     8461 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
     8521 attagcgatg atgatctacc gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa
     8581 attagtctga cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg
     8641 ggcgtcactg aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc
     8701 gtgtatcttg tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga
     8761 ttgactaaag tcacagtagc agcgacagct gttatactta cggctattct accgctttat
     8821 atgtagagtt aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa
     8881 aaagaggagt tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa
     8941 tattagttat ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca
     9001 tttttaagca tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga
     9061 atggtttgaa ttgtctcata tgcggacaaa aggcttactc cttttataga ttttagcact
     9121 tacattcata agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct
     9181 tgactattat agcatatcta aaatcgaata aaaattctca attttaactg tttttgctat
     9241 aattttagcg aggtgatcgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     9301 ataaaaacac aaccattatt ggttcgcttt actttacaaa caattcatga atcgattaat
     9361 tgtgtagttg cagatattga aattatcgat aaattgttaa ttatggatga tggaaaatac
     9421 aatattgccg tcacaggtca tttcaataaa cggaatcaat tagtcattga atcgatgcaa
     9481 attagaaacc ctgatcattt tacgcgatca atgggcatat aaataaaaga agagctgcaa
     9541 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaggaagaa agggaaaggc
     9601 ctgtttagga aggcacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc
     9661 accgccgttc gagaagaaag gaagtgaacg ttgcacgttc actaagataa ggggtcttgc
     9721 tttgtaagac aaaataggaa aagcttcctc tcaccaaaag gttcgttcct gttcgctatc
     9781 tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga
     9841 caagggtaaa tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga ttcattctgg
     9901 ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc
     9961 agaatcaatc atgcgaaaga aatttgatta tttgggtgtt cccttcagcg aattattccc
    10021 aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga aatgtatcaa
    10081 aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga
    10141 ttatattttg attgaggaga aaggaaagaa acgctgatga aaacacaaca caaattattg
    10201 actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca
    10261 gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg
    10321 actatgctat ttgaagacct agtgaagatt gcaggatgtg aactgcatta tcaagaaaac
    10381 ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa aggagaaaaa
    10441 aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
    10501 aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa acattataaa
    10561 gaaaaattgt cggtagctgt ggaagaaatg attgaatcag gtgtcgaaat gaatcttatt
    10621 gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg gtttttccaa
    10681 gccttactat gctttgaaat aggtgtcaaa ccaagcacta ttaaaccttc tgaataccag
    10741 gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
    10801 gaatggttag attattttga aaatatcaat aagtatggcg catattacac catgaaaaag
    10861 gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca tatcaacgat
    10921 ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg tggaataagg
    10981 acaaaaaaag cctagtaact aagggaatgg ttcttatttc gcaccaaccg cccccgccgc
    11041 ttaggtggtg cgaaataaac gggcaaagcc cgataactta agagaaagaa ggtgatccat
    11101 ttgaaaaaga ttattccctt attgttagta ctgtttgttg gcgaaatact tatgcgagat
    11161 agtgcagctg tacagttaaa cctcgctgta tttttggtaa tactggtgtt ttgtttgatc
    11221 ttttggcggt ttgtattagt cctcgtgagt ctactattca agatgattgc agccatattt
    11281 ttcatggggt tatttcgatg aaaggagaga gtaccaatgc caattgcaat aggaaataaa
    11341 cggttacccg tgacattaga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag
    11401 tacggcaaaa gtgaatccag gattatgtgt attgcgttag atttattgat tgcctgtatt
    11461 ggacaatatc tctattgtat tcgaggaaga aagctttacg cttgaatgcg ataagttaac
    11521 ccgatatatc gatccaaaat tctatgagga taaaaacctg gcatgggaac tggctttata
    11581 gacagagtgc tagtgcaagt aaaggaagaa aggaagatca ccatgaaata caatttatta
    11641 gaccataagc gaatgtgggt agagaaatta gaggaagact tagaacagtt ggaattgtta
    11701 ggtcataata agacttcaac ggtgtataaa gaaggactaa aacgaacgaa aaaagcacaa
    11761 aaagaactag caacactgga aagtagggtt cagaaaaaag aagtagactt aacacacact
    11821 tacctttagg agtagaaaat ggcagacaag agaaaagaaa agattcaaaa attactggcg
    11881 cttgcggcgg atgtaaatga tgaggaatca gtgagcgcat tagccaaagc ccagcaattg
    11941 atgatggact atgatcttac tgaagatgag attttcaatt atcatgagca gcaacggata
    12001 gaagcagtag aaacgcacgt gatctatcgt ggacgtccgc aaaaatggct gtatcgttta
    12061 tttagaaatt aagataaaaa aggctagtta acaggtttgt tgactggcct tttttgatat
    12121 gtttgtacat gttttattgt gttttaattt ttctcttttt tatagcaagc tccttttata
    12181 ataaatattc cgaaaagtga agaccatttt ttatttatta agactattat tattagtgag
    12241 ataaaatttg attgatctta cagaatattg gtgacgatta tgacactaaa gatactattt
    12301 atgaaaattt tgcaaaaatg ttctgaaaat tacatataat tgttttataa aaaagggagg
    12361 caattatatg aagaaaaaat ttgttagtat tttttgattt taggaattgt tttattgagt
    12421 gtatctactt taggaattac agtagatgct gcaacttatt atggaaatgg tgtttattgt
    12481 aatactcaaa aatgttgggt agattggagt agagctcgct ctgagattgt tgatagagga
    12541 gtaaaagctt acgttaatgg ttttacaaaa gtgctaggtg gagtcggtgg aagataaaaa
    12601 agaaaaactt tttgacttaa ttgcgaatgc atataattca aagtcatata ataatgtaga
    12661 agtaaaagaa ggatttcttc aagatgctaa gaaaatacga gaggataaac catatcctga
    12721 aattattata tcagtgtaca aacatttatc taaattaaaa gctgaggaac g
//
LOCUS       DACIFS010000072        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.072, whole genome shotgun sequence.
ACCESSION   DACIFS010000072 DACIFS010000000
VERSION     DACIFS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.072"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            6..305
                     /locus_tag="IYA83_002211"
     CDS             6..305
                     /locus_tag="IYA83_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587626.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IYA83_002212"
     CDS             378..1739
                     /locus_tag="IYA83_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ8587627.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IYA83_002213"
     CDS             1761..2021
                     /locus_tag="IYA83_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587628.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IYA83_002214"
     CDS             2035..2787
                     /locus_tag="IYA83_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ8587629.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IYA83_002215"
     CDS             complement(2881..3486)
                     /locus_tag="IYA83_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587630.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IYA83_002216"
     CDS             complement(3539..4513)
                     /locus_tag="IYA83_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ8587631.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IYA83_002217"
     CDS             complement(4891..5670)
                     /locus_tag="IYA83_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8587632.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYA83_002218"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYA83_002218"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ8587633.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IYA83_002219"
     CDS             7161..8558
                     /locus_tag="IYA83_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8587634.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IYA83_002220"
     CDS             8639..9079
                     /locus_tag="IYA83_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ8587635.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IYA83_002221"
     CDS             9227..9553
                     /locus_tag="IYA83_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ8587636.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IYA83_002222"
     CDS             9554..10303
                     /locus_tag="IYA83_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587637.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IYA83_002223"
     CDS             complement(10383..10838)
                     /locus_tag="IYA83_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ8587638.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IYA83_002224"
     CDS             complement(11265..11540)
                     /locus_tag="IYA83_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587639.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IYA83_002225"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IYA83_002225"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ8587640.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACIFS010000073        12574 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.073, whole genome shotgun sequence.
ACCESSION   DACIFS010000073 DACIFS010000000
VERSION     DACIFS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.073"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..120
                     /locus_tag="IYA83_002226"
     CDS             <1..120
                     /locus_tag="IYA83_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ8587641.1"
                     /translation="YLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(444..527)
                     /locus_tag="IYA83_002227"
     tRNA            complement(444..527)
                     /locus_tag="IYA83_002227"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Leu,seq:cag)
     gene            1055..1555
                     /locus_tag="IYA83_002228"
     CDS             1055..1555
                     /locus_tag="IYA83_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587642.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1745..2089)
                     /locus_tag="IYA83_002229"
     CDS             complement(1745..2089)
                     /locus_tag="IYA83_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ8587643.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2243..3163)
                     /locus_tag="IYA83_002230"
     CDS             complement(2243..3163)
                     /locus_tag="IYA83_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ8587644.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3350..3841
                     /locus_tag="IYA83_002231"
     CDS             3350..3841
                     /locus_tag="IYA83_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ8587645.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3865..6240)
                     /locus_tag="IYA83_002232"
     CDS             complement(3865..6240)
                     /locus_tag="IYA83_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ8587646.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6566..7192
                     /locus_tag="IYA83_002233"
     CDS             6566..7192
                     /locus_tag="IYA83_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ8587647.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7215..7919
                     /locus_tag="IYA83_002234"
     CDS             7215..7919
                     /locus_tag="IYA83_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ8587648.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8111..8944
                     /locus_tag="IYA83_002235"
     CDS             8111..8944
                     /locus_tag="IYA83_002235"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ8587649.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9141..11069
                     /locus_tag="IYA83_002236"
     CDS             9141..11069
                     /locus_tag="IYA83_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ8587650.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11207..11662
                     /locus_tag="IYA83_002237"
     CDS             11207..11662
                     /locus_tag="IYA83_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ8587651.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12132..12401
                     /locus_tag="IYA83_002238"
     CDS             12132..12401
                     /locus_tag="IYA83_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587652.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3843 a         2340 c         2280 g         4111 t
ORIGIN      
        1 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
       61 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      121 gattcattat ttgaacgttt aaacacattc aaaatatagt aagaggtcat gcgatttatt
      181 ctttcgcatg acctcttttt gagtttaagt ttagtcttta ttccttaact atcgtttgcc
      241 agtatttctt gtatcagaat ctataaatct actatttgaa tcaatctatt aggttttaaa
      301 ctgtcccacc ttcacacaaa ttttttttga tttatttgaa tctggttccg ttgtttaaaa
      361 tttttcctat attcttttag ataatatgtt attttctttc tcatcgctcc aggtgtacct
      421 tctctttata aaaaaacaaa ttatgcggct aagaagactc gaacttccac gaggtcaccc
      481 tcactagcgc ctgaagctag cgcgtctgcc aattccgcca tagccgcatc acatctttta
      541 gtttaggatt acatgtgttt tttgtcaaga acatattttt gttctttttc atttctgtac
      601 gccctccctt atgccatcta cacaaataat tcatccgagc aactccatca tctattgagg
      661 gttggtgaaa aaatctgttt tatccatatg atcgatttca atgttaaacg aacgactctg
      721 ggattagtaa ctattttatt tttttcttca tttctttttt cctctttttc tcgattcagc
      781 tatcttcttt catttggata ttgattattt cttctgttga ttactatcct tctggaattc
      841 tagacgctga agtaattcac gttttctttc ttctagagat cataagtaac gaatgaacga
      901 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
      961 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
     1021 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
     1081 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
     1141 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
     1201 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
     1261 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
     1321 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
     1381 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
     1441 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
     1501 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
     1561 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
     1621 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
     1681 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
     1741 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
     1801 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
     1861 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     1921 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     1981 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     2041 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     2101 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
     2161 aagtaagatt ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt
     2221 attctaaaat aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata
     2281 cgccagttgt tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat
     2341 gagttggtaa aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat
     2401 catacatttc gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc
     2461 caccgacaga cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta
     2521 cctcttctgg cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg
     2581 catcaattac ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt
     2641 taaagaagaa gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga
     2701 ctgtttttcc agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa
     2761 agcccatttt taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat
     2821 tcatcaaaat attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac
     2881 atacaacatt cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata
     2941 caaacacagg gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc
     3001 attcatataa aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat
     3061 tggctggaat caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa
     3121 ccactatttt ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca
     3181 cctctcccct actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat
     3241 acttcgaact caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg
     3301 ataagataga tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga
     3361 tgctagcttc gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca
     3421 aacaacaaaa gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt
     3481 agagaacagc aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga
     3541 tgtattattt ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga
     3601 atcagaacac gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga
     3661 tcgtgaaaac gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc
     3721 acaaatccaa gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt
     3781 gattgcttta ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta
     3841 atcaaaaaac gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga
     3901 taaccaaaaa cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt
     3961 ttgccaatgt tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa
     4021 tacaacgatt ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt
     4081 gttcattcca ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata
     4141 tgatttgatc agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact
     4201 gccgatcaat cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca
     4261 aaatccaatc aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc
     4321 ttcctcattc atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa
     4381 tgcttccacc gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac
     4441 ctttccatca gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt
     4501 tcgataagtc gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg
     4561 actcggcagt ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag
     4621 tggtgaaaaa ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag
     4681 aaacaagctt ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac
     4741 tgttgtttcg tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa
     4801 ataataagga agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt
     4861 aattttttcg cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg
     4921 atgataatcc caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag
     4981 tttgttgaaa cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt
     5041 cgttttttca taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg
     5101 tgcttcgtca atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa
     5161 ataaacagtt ggatcaaata aataattgta atccccaatg attacgtcac accagatact
     5221 gacatctaat gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac
     5281 ttcacgagtg atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt
     5341 gtaataacca ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca
     5401 gatcttgtct ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc
     5461 tgtcatagca tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt
     5521 ttctccctct tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc
     5581 tgttccggta ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc
     5641 taccgctagt tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa
     5701 agaggcattg cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc
     5761 agtcagcttt ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt
     5821 gatcacttct tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg
     5881 ttcttgacta tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc
     5941 tggttctaaa tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc
     6001 gtctcttttg aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg
     6061 aactgttgtt tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt
     6121 gcggtggatt ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact
     6181 tccacgtcgc aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat
     6241 ttccaccacc tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc
     6301 tctttccaaa cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg
     6361 gatttttgga tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc
     6421 cttctcttta gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag
     6481 aaataagtac ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag
     6541 ttttggctat caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga
     6601 agaaaactgt tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga
     6661 tgcggaacga atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct
     6721 tacccatact cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat
     6781 cccagtttat gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc
     6841 tggactcggt agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt
     6901 tgaaatgaaa acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca
     6961 ttctatagga agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt
     7021 tagaggaagt atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag
     7081 tattcggacc cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga
     7141 tgcaacgacg attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt
     7201 aggaggagaa taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt
     7261 tcaatacaat tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag
     7321 aaggcattca ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta
     7381 gtgacagtgg tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa
     7441 aagaaattcc ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag
     7501 gtggatatga catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg
     7561 atgaaatgtt taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag
     7621 ataccgctgg gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg
     7681 aagatatcgc ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg
     7741 gtttgacgat cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc
     7801 ttttaaatgg cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca
     7861 tcaatgatac gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat
     7921 ggggatattt taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt
     7981 cttctttttt ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa
     8041 aaacagattg ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag
     8101 gaaggaattt atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg
     8161 aataacggaa tgggtgccca tcagtagtac tggtcatttg atcctagcag atgaattcat
     8221 caaactggat gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc
     8281 gatcttggct gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc
     8341 aacggtagaa aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc
     8401 agccatcatc attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt
     8461 accagttgcc attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa
     8521 caaaaacaaa acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct
     8581 tgttgggtta ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat
     8641 cctaggatct atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt
     8701 agggattcct gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg
     8761 gaattcattc aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt
     8821 ggtttccgtc ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc
     8881 ttttggctgg tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat
     8941 gtagaaaaaa ccagaacact tctcttattt gagaaatgtt ctggtttttt attttcttta
     9001 ataataatca ttataaataa taaaaacaac atcaagctta atcaccaaat gataaccatt
     9061 atcgttctca tttagctgtt attattatca attacatttt tattatgcta tccttgtttt
     9121 gacaaggagg tctaataacg atgactaatc agaaaaaagc ttttgcagca acaatcggct
     9181 gccttttatt tatgatcttc ggttttatct tcgaccgatt ctctcttacc atcaccccac
     9241 ttttttatat cagtgccatt atcatcggtg gatataaaca aacatcggaa ggcttgaaag
     9301 aattgtggaa tgatcggaca ctgaatgtgg atttattgat ggctttagct gctattggtg
     9361 cctgtttgat cggtaattac tttgaaggag caatgttgac attcatcttt tgtttaagcg
     9421 gtgcattaga ggaatacaca actaataaaa gtcaaaaaga aattactgct ttgatgaatc
     9481 ttcaaccaca aaaagcccaa ttattaaaag aaaatggaca aatgcaagaa gtagatgttt
     9541 ctcaactaaa ggtggataac ttggtatttg tcgctaaagg agcagctgtt ccgatagatg
     9601 gctttttaga aagcacacag gcaacgatcg atgagtctgc attaagcggt gaatctgtgc
     9661 ctgtagaaaa atatttaggc gatgagatgt atgcaggaac gctgaatcaa ggaaatccca
     9721 tcacgattcg agtaacaaaa acgagttccg aaactgtctt tgctaagatt atccaattag
     9781 tagaagaagc gcagaatacg ccaactcaaa cagcttcatt tatcgaacga atcgaaaata
     9841 attatgtaaa attgattttg cttgctgttc ctctaatgat ccttctcccc cactttttcc
     9901 taggttggag ttgggatgaa agcttttatc gaggaatggt attattagtt gtggcttctc
     9961 cttgtgcgct tgtcgcatct gctacaccag cttctctagc tgctctttca aattctgcaa
    10021 aaaatgggat tttaatcaaa ggcggtatcc atttagaaaa gctttctgaa ttaaaagcga
    10081 ttgcctttga taaaacagga acgctcacta aaggaaaacc agttgtaact gacagctttt
    10141 tctttgatga aaaggatttg gcacaacaac ttcttgttgc tatggaacaa aaaacgacgc
    10201 atccacttgc tcatgcgatt ttgcaatatt tcgattgcac aatacctgaa gaaatccaat
    10261 acttaccaat agaagaaatt actggttttg gattacaaac tacctattta ggtgcgcaat
    10321 ggaaagttgg caagcacgca tatgatcctg agacgatgat aatcagcaaa gagattgcgg
    10381 aaatgatcga acgtttggaa aatcaaggca aaaccgtgat ttatctttct aaagatcagc
    10441 agttaattgc tgttttaggt ctgctagata ttccaaaagc aaatacccaa caagtaattt
    10501 cctactttaa atcccaaaac atccatacaa gtatgataac cggcgaccat tctggcacag
    10561 caaaggccat tgctgaacaa gtagggattg aacattacta tgcaagctgt actccggaag
    10621 aaaaaacaca actagtcaaa aaagagaatg aactttacca agtaaatgcg atggttggtg
    10681 atggagtcaa tgatgcgcct gctttagcag ccgcttcttt aggtgtcgct atggggcaag
    10741 ggacagatgc cgctatggat atcgcagaca tcgtcttaat gaaaagcgac ttaaataaat
    10801 ttgtctacag tcatcggcta tctttaaaaa tgcggcggat catcaaacaa aatattattt
    10861 tttcgattct tgttattctc ttgttgattg cttctaactt cctgcaattt ttaaatctac
    10921 ctcttggagt tgtaggacac gaaggaagca caatattggt tattctgaat ggcttgcgtt
    10981 tactaagacc tttacctgat ctttctgatt cttcaaaaaa atgtaagtct tgtccactat
    11041 acaaagcgcg gctgtcttct gtcaaataag caaagtgttt ttttatgatt ttgtttattt
    11101 tatgaaaaaa acagcttatg aataggtctt gaaggatgaa ttcctacttt tgtaactttc
    11161 taaaaacatg ttatgattgt ttcgaagaaa gtgaggaata tatttcatgg aaagttggct
    11221 ttttttagga attattttac ttgtagcaat tttagctaaa aatcaaagtc tgatgatagc
    11281 ggcagctttt gttttgatta tgaagctcat accagcctcc cgttcctttt tcccttggct
    11341 tcaaggcaaa ggaatcaata ttggggtgac gatcatcaca gcggctattc taatccctat
    11401 cgcaacagga gaaatcggtc tacgtgatct attagaagcc tttaaatccc ctattggctg
    11461 ggtagctgtt ttttgcggtg ggctagtggc tgtcttatct tccaagggtg taggattgat
    11521 tgcttctgat ccacaaatca ctgttgcact tgttttcggc acgattttag gtgttgtctt
    11581 cttaagagga atcgccgccg gaccggtcat tgcttctggt atcacttact gtatcttaca
    11641 agtatttgct ttaggaaaat agaacaaatt ttgtctattt taggagattc ttaacacccc
    11701 caatttactg atccgtgata tactgacaac gaggtgatac aatgttcgaa aaattaaagt
    11761 atttcatagg aacaatcggc tggtttcttc tagcggtact tttgattctg ggcttagctt
    11821 atctattatt tcctgatttg atacaaaatg tgtttgatac tgctttttga ttgacctatt
    11881 aaaacgatat aggggatgct cctgcgatgc aggggctttt tttaatcaat gttttgtaat
    11941 aatttaggcc gtgccaatga gacttcctat agaattcgat gctctttttt ctttttggct
    12001 cttctgctct tttttgttat actagattac agtggacaaa agaaagaatg atcgcatcca
    12061 cttcaacgaa tatcaaaatg gaagaattag tgcctgtttt caggttactt tgaaaagaaa
    12121 ggaagacgct tgtggaaact tttgaaggtc ttttaattga tacgtacgtt ttacaagaac
    12181 agccaatgat gctgactggg atcaacttgc agcttgagga ccgtgtgatc aatggcctgc
    12241 ttaaagatcg ctatttggca aaatatatct tacgtttgcc cctaaataaa tatgtgctgg
    12301 aagtaagtgg caaatggaac cgtcggaacc agttggtcat aaaaaaaatg ttgataaaaa
    12361 atatcgatga atacattaaa ataatcggta caccagaata gcctttactg atcaaaggat
    12421 actggttata aaataactgc agtttcttaa tagaaaaaca aagaacaaca gggaatcgta
    12481 tcccttttca aaaaaaactt tgcaaacggt tctctttgtt atataataac ttagtaccaa
    12541 acattcccat tcaaactttg tttaaataga taaa
//
LOCUS       DACIFS010000074        11593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.074, whole genome shotgun sequence.
ACCESSION   DACIFS010000074 DACIFS010000000
VERSION     DACIFS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.074"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            396..602
                     /locus_tag="IYA83_002239"
     CDS             396..602
                     /locus_tag="IYA83_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ8587653.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(804..1805)
                     /locus_tag="IYA83_002240"
     CDS             complement(804..1805)
                     /locus_tag="IYA83_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587654.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1810..3723)
                     /locus_tag="IYA83_002241"
     CDS             complement(1810..3723)
                     /locus_tag="IYA83_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ8587655.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3891..4397)
                     /locus_tag="IYA83_002242"
     CDS             complement(3891..4397)
                     /locus_tag="IYA83_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ8587656.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4557..4997
                     /locus_tag="IYA83_002243"
     CDS             4557..4997
                     /locus_tag="IYA83_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ8587657.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5023..6180)
                     /locus_tag="IYA83_002244"
     CDS             complement(5023..6180)
                     /locus_tag="IYA83_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587658.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6183..6539)
                     /locus_tag="IYA83_002245"
     CDS             complement(6183..6539)
                     /locus_tag="IYA83_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587659.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6837..7811)
                     /gene="sgrA"
                     /locus_tag="IYA83_002246"
     CDS             complement(6837..7811)
                     /gene="sgrA"
                     /locus_tag="IYA83_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ8587660.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8007..8846)
                     /locus_tag="IYA83_002247"
     CDS             complement(8007..8846)
                     /locus_tag="IYA83_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ8587661.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9031..9918
                     /locus_tag="IYA83_002248"
     CDS             9031..9918
                     /locus_tag="IYA83_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ8587662.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNNDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10512..11207)
                     /locus_tag="IYA83_002249"
     CDS             complement(10512..11207)
                     /locus_tag="IYA83_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ8587663.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11191..11589)
                     /locus_tag="IYA83_002250"
     CDS             complement(11191..11589)
                     /locus_tag="IYA83_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ8587664.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3458 a         2305 c         1735 g         4095 t
ORIGIN      
        1 ttctaaaaaa ttaaaaaaaa ttttggtgaa ttaagtattt ccttgataac actggctttt
       61 ggcctcattt taggttatca tttgtaattt tatttcacag ttgatatgat tgttatgtaa
      121 aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat
      181 cttttctatt atattcccat attttctatt attggtctag acgtatgatg gtaaactttg
      241 tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg
      301 ctagatgatc attttattca catcctaaca aacttgtcac atttatagat tcttaatcta
      361 tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc
      421 tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca
      481 ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc
      541 ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct
      601 gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc
      661 gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa
      721 ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac
      781 aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt
      841 tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc
      901 tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct
      961 tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa
     1021 cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta
     1081 ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact
     1141 tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg
     1201 ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac
     1261 aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa
     1321 tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact
     1381 ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc
     1441 gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat
     1501 gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag
     1561 atatccatat tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt
     1621 tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg
     1681 tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat
     1741 acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat
     1801 ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa
     1861 aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct
     1921 tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc
     1981 acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc
     2041 ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt
     2101 ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt
     2161 cttttgctct gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc
     2221 tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat catggaacac
     2281 tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt
     2341 tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata actcatataa
     2401 tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg
     2461 caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg
     2521 atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat
     2581 gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt
     2641 aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt
     2701 gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata
     2761 aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc
     2821 atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga
     2881 atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat
     2941 cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg
     3001 atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg
     3061 atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc
     3121 tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag
     3181 ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag
     3241 ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat
     3301 ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt
     3361 accgatatca tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc
     3421 gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt
     3481 cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt
     3541 ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc
     3601 ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt
     3661 acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt
     3721 cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa
     3781 ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc
     3841 agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc
     3901 aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct
     3961 ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct
     4021 aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat
     4081 tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa
     4141 ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc
     4201 tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt
     4261 ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat
     4321 tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg
     4381 tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt
     4441 atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact
     4501 tttcgactat ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga
     4561 tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta
     4621 tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg
     4681 aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt
     4741 ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata
     4801 ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa
     4861 aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa
     4921 agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc
     4981 tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa
     5041 tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg
     5101 cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc
     5161 atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga
     5221 tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg cccataattc
     5281 ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa
     5341 aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt
     5401 attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat
     5461 cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt
     5521 cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata
     5581 atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga
     5641 cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc
     5701 caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat
     5761 gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc
     5821 ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga
     5881 gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc
     5941 tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt
     6001 tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa
     6061 aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga
     6121 taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa
     6181 acttatataa ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa
     6241 tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa
     6301 aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat
     6361 tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg
     6421 gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa
     6481 caatagttca aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa
     6541 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
     6601 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
     6661 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
     6721 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
     6781 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
     6841 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
     6901 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
     6961 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
     7021 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
     7081 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
     7141 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
     7201 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     7261 tcgctgctcg tgttttctgt gctactttca ctgctcgtac tctctgtact gctttcaccg
     7321 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
     7381 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
     7441 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
     7501 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     7561 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     7621 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     7681 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     7741 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     7801 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     7861 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     7921 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     7981 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     8041 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     8101 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     8161 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     8221 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     8281 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     8341 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     8401 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     8461 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     8521 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     8581 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     8641 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     8701 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     8761 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa ctaagactat taaaacagat
     8821 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
     8881 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
     8941 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
     9001 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
     9061 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
     9121 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
     9181 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
     9241 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
     9301 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
     9361 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
     9421 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
     9481 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
     9541 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
     9601 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
     9661 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
     9721 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taatgacaaa
     9781 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
     9841 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
     9901 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
     9961 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg
    10021 gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat
    10081 ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat
    10141 gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc
    10201 gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt
    10261 ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg
    10321 aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat
    10381 acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat
    10441 gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg
    10501 gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg
    10561 aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct
    10621 aatacttgat ctagtagctc tttacctaac gttatcgcat gagctccaga tcgaatggct
    10681 gtagtgactt gttcacaatt tttgaaacta gctgctaata tttttgttgg tctttgcgta
    10741 cgttcgatct cagtccgcaa cgaatcaatc actttcatag catcaatgcc ttgattttgc
    10801 atcctgttat aataaggtgc aatataatct gctccattat taatagctaa atacccttgt
    10861 aattctgtgt aaatagctgt accggtaatc aaataacctt cttttttcaa ctttttgatc
    10921 gcttgaaggc ctgcttgatt caccggcacc ttgataaacg tatcttttcc cagttcttct
    10981 atgataactc gagcatcctc tatcatttct tcttccgttc ttcctattgt ctgaatatgg
    11041 ataggaatct gtccgattat ttttttgatt tctttcatat gcgagaagaa gtttgacgct
    11101 ccttcttttt tcagaattgt aggatttgtt gttacgcctg ttaaattcaa aaactgttga
    11161 caagaataaa tactttttgt atttgctgta tcaagcatga tttccatata gctttccccc
    11221 attctgatcc tcttgatcga ttttaatgat tgtccccttt cttaaatgcg gcatttcctt
    11281 atcttctaca tacattgttc ctggaagttc cggctcatga gatccatcaa attttatcgt
    11341 gatatgtcct aattcattca aattagtttg gacaacatct cctactgctg tgattacata
    11401 catttgttta tcaaaacaga gcttcatatc acaagttatt tcttttctta cctgattcaa
    11461 ttcaatctta taacaataat ctgttaatgt ttccggtgct tcatctccaa ataaaatcat
    11521 tattttttct ttcctgaata cttctgcttc tataccaaca tctgttacaa ccgtttcaaa
    11581 gatcgtcatc tct
//
LOCUS       DACIFS010000075        11344 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.075, whole genome shotgun sequence.
ACCESSION   DACIFS010000075 DACIFS010000000
VERSION     DACIFS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.075"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYA83_002251"
     CDS             complement(3..2012)
                     /gene="metG"
                     /locus_tag="IYA83_002251"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ8587665.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2280..2558
                     /locus_tag="IYA83_002252"
     CDS             2280..2558
                     /locus_tag="IYA83_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587666.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2655..3476
                     /locus_tag="IYA83_002253"
     CDS             2655..3476
                     /locus_tag="IYA83_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ8587667.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3761..4771)
                     /locus_tag="IYA83_002254"
     CDS             complement(3761..4771)
                     /locus_tag="IYA83_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8587668.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4783..5736)
                     /locus_tag="IYA83_002255"
     CDS             complement(4783..5736)
                     /locus_tag="IYA83_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8587669.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5726..6796)
                     /locus_tag="IYA83_002256"
     CDS             complement(5726..6796)
                     /locus_tag="IYA83_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587670.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6802..7845)
                     /locus_tag="IYA83_002257"
     CDS             complement(6802..7845)
                     /locus_tag="IYA83_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587671.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7846..8787)
                     /locus_tag="IYA83_002258"
     CDS             complement(7846..8787)
                     /locus_tag="IYA83_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ8587672.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8871..9218
                     /locus_tag="IYA83_002259"
     CDS             8871..9218
                     /locus_tag="IYA83_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ8587673.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9288..10949)
                     /locus_tag="IYA83_002260"
     CDS             complement(9288..10949)
                     /locus_tag="IYA83_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ8587674.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3209 a         2305 c         2059 g         3771 t
ORIGIN      
        1 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
       61 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      121 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      181 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      241 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      301 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      361 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      421 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      481 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      541 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      601 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      661 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
      721 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
      781 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
      841 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
      901 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
      961 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1021 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1081 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1141 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1201 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1261 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1321 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1381 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1441 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1501 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1561 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1621 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1681 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     1741 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     1801 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     1861 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     1921 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     1981 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2041 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2101 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2161 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2221 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2281 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2341 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2401 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2461 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2521 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2581 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2641 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     2701 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     2761 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     2821 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     2881 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     2941 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3001 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3061 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3121 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3181 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3241 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3301 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3361 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3421 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3481 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3541 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3601 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3661 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     3721 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     3781 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     3841 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     3901 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     3961 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4021 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4081 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4141 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4201 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4261 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4321 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4381 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4441 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4501 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4561 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4621 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4681 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     4741 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     4801 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     4861 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     4921 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     4981 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5041 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5101 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5161 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5221 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5281 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5341 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5401 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5461 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5521 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5581 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5641 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     5701 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     5761 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     5821 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     5881 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     5941 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6001 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6061 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6121 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6181 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6241 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6301 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6361 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6421 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6481 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6541 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6601 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6661 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     6721 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     6781 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     6841 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     6901 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     6961 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7021 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7081 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7141 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7201 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7261 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7321 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7381 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7441 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7501 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7561 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7621 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7681 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     7741 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     7801 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     7861 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     7921 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     7981 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8041 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8101 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8161 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8221 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8281 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8341 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8401 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8461 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8521 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8581 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8641 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     8701 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     8761 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     8821 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     8881 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     8941 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9001 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9061 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9121 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9181 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9241 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9301 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9361 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9421 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9481 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9541 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9601 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9661 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
     9721 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
     9781 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
     9841 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
     9901 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
     9961 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10021 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10081 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10141 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10201 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10261 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10321 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10381 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10441 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10501 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10561 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10621 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10681 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    10741 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    10801 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    10861 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    10921 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    10981 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11041 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11101 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11161 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11221 aaagtcttgt cacagtccct tatctgaata aacggtgttc aaaagccagt ttctcggtat
    11281 taagccaaat ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg
    11341 aaac
//
LOCUS       DACIFS010000076        11119 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.076, whole genome shotgun sequence.
ACCESSION   DACIFS010000076 DACIFS010000000
VERSION     DACIFS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.076"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            102..338
                     /gene="secG"
                     /locus_tag="IYA83_002261"
     CDS             102..338
                     /gene="secG"
                     /locus_tag="IYA83_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ8587675.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            450..1196
                     /locus_tag="IYA83_002262"
     CDS             450..1196
                     /locus_tag="IYA83_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ8587676.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1199..3556
                     /gene="rnr"
                     /locus_tag="IYA83_002263"
     CDS             1199..3556
                     /gene="rnr"
                     /locus_tag="IYA83_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ8587677.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            3578..4042
                     /gene="smpB"
                     /locus_tag="IYA83_002264"
     CDS             3578..4042
                     /gene="smpB"
                     /locus_tag="IYA83_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ8587678.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4344..5063
                     /gene="atpB"
                     /locus_tag="IYA83_002265"
     CDS             4344..5063
                     /gene="atpB"
                     /locus_tag="IYA83_002265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ8587679.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5116..5331
                     /gene="atpE"
                     /locus_tag="IYA83_002266"
     CDS             5116..5331
                     /gene="atpE"
                     /locus_tag="IYA83_002266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ8587680.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            5422..5946
                     /gene="atpF"
                     /locus_tag="IYA83_002267"
     CDS             5422..5946
                     /gene="atpF"
                     /locus_tag="IYA83_002267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ8587681.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5933..6475
                     /locus_tag="IYA83_002268"
     CDS             5933..6475
                     /locus_tag="IYA83_002268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ8587682.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            6498..8054
                     /locus_tag="IYA83_002269"
     CDS             6498..8054
                     /locus_tag="IYA83_002269"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ8587683.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            8068..8970
                     /locus_tag="IYA83_002270"
     CDS             8068..8970
                     /locus_tag="IYA83_002270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ8587684.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            8993..10399
                     /gene="atpD"
                     /locus_tag="IYA83_002271"
     CDS             8993..10399
                     /gene="atpD"
                     /locus_tag="IYA83_002271"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ8587685.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10413..10835
                     /locus_tag="IYA83_002272"
     CDS             10413..10835
                     /locus_tag="IYA83_002272"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ8587686.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3684 a         1982 c         2455 g         2998 t
ORIGIN      
        1 ttttgaactt tcagctgtta ttccatttat atagagttgt cacggacttt tatctatgat
       61 aaagttaaag agacgatatc ttttgggaag gaggctgcga tatgtataat ttaattttgg
      121 gaatcgtgat cgttttatcg attgttatgg tcattgcgat tatgatgcag ccaagcaagc
      181 aaaatagtgc agcgagtgca tttaccggcg gtgcggatca actattcggc aaacaaaaag
      241 ctcgcggctt tgaagctgtc atgcagcgat caactgcagt tatgggagct atctggatga
      301 tcttattatt tgtcctagca tatttatctt cgaagtagtt gaataagcct ctcgcaaaac
      361 ggcgggcggc tttttttatt gcaaagagaa cgcttaagaa gaagttatga aagaaactat
      421 ggtaaaatga ggcgaaagag gtgagacgag tgagacaatt accaaaaccc ctttatgcaa
      481 aacatggaaa gcgagcggtt ttgctgcttc acgcttactc aggaagtcca aacgatgttc
      541 gaatgctcgc acgatttttg gaaaaagcag attatacagt ttatgcgccg ctttttaaag
      601 gacacggaac aatggagcca tatgatatct tgcaagaaaa agcagaaagc tggtgggcag
      661 acactaaaaa agccatccat tttttacagt ctgagcagtt ttctgatatt gccgtattag
      721 gattatcaat gggcggcatc tttgctgtac gagcactaga agaagaatca gtgattggcg
      781 gcggtttttt ctgttcgcct ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa
      841 aatacgtacg tcaagtgctg aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag
      901 cagttgctta ccgctcgtta gctgaacaac aattaatgga tatacaagat caggcagcaa
      961 tcgtagaaag taggttgtca gatatccaac agccgatatt tttagctcaa gctgggaaag
     1021 atgaaatgat cgaccctaat ggcgtatttg aaactgcacg caagttgagc cgtcagcgag
     1081 tgacacttca atggtatcct gaaagtggtc atgtgattac agttggtaca gcaagacgcg
     1141 aattagaaaa agacgtcttg gaatttttag aaaaattacc ttggaatgag gaataaatat
     1201 gacaaaagaa acgataaaag agaaaattct ctttttcatg gaaagcagtc gcaaacgcag
     1261 tttttctatg gaagaaatcg ctgaaggttt agggttccaa aaaagcgatg attttaaatt
     1321 actagtacaa acgattgctc aaatggaaag agaacagagt atagttttca ataaaaaagg
     1381 aaaagtgaaa cttcctttaa aaccaatctt gatcgaagga acatttcgag caaatgaacg
     1441 aggatttgga tttgtaacga ttgatcccga agaagatgac atctatatcc caaaagaagc
     1501 gactggtttt gcaatggatg gagacaccgt agcaatcgat atcatgaagc gagcaaatac
     1561 agatatggat cgcggagcag aggggaaagt agtcgagatt cgagaccggg cgactactca
     1621 gttagcaggg gaattcgttg cttattcaga agaagaaata caagaaacag atctttacgg
     1681 cgttgttatc ccgaaagata gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg
     1741 agttcgtcca gttgacggaa gtattgtgct gatcgaactg acccattacc ctgaaaaagg
     1801 gtatgcgact agtttagaag ggataatcaa gcaagtgatt ggtcataaga atgaccctgg
     1861 catggatatc ctctcaattg tcgtggctaa tggtatccct acgaaatttc ctgatgatgt
     1921 attgtctgaa gcagatgaag ttccagatca aatcagcgaa aaggatataa ctggacgtag
     1981 agatttaaga aatcagctca ttgttacgat tgatggagaa gatgcaaaag atctagatga
     2041 tgcagttacg gttaaaaaat tagataacgg taattatttt cttggagtac acattgcaga
     2101 tgtttcttac tatgtgacac aaggcagtca attggacaga gaagcatatg aaagagggac
     2161 aagtgtatat ttgactgatc gagtgattcc gatgatcccg caacgtttat cgaatggtat
     2221 ctgttcactg aatccccatg ttccaaggct gaccatgagt tgcgaaatgg agattgatcc
     2281 aaatggacaa gtagtctctc atgatatttt cccaagtgtg atccaaacaa cagaacggat
     2341 gacctacaca gctgtcaatc aaatcttaga agatcaagac gaagaaacga tgcaacgcta
     2401 tgctcagctc gtacctatgt tccaagaaat gggtgaattg catcgaatcc ttgaaacgat
     2461 gcgaatcaaa cgtggcgcga tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc
     2521 tgaaggacac ccaaatgaca tccagctaag aagcagagga attggtgaac gcttgatcga
     2581 atctttcatg cttgcggcca atgaaacggt agctgaacat tataataaat taaatctgcc
     2641 gtttatttac cgaatccatg aacagccaaa agaagaaaaa atgcagcgct tctttgattt
     2701 tgcttcagct ttgggcattc tggtccgagg aacgaaagga acgatcacac caaaagattt
     2761 acagcatgtg atcgaaggag tagaagacaa accagaagct gcagttatca atactatgtt
     2821 gctaagaagc atgcaacaag cacgctattc agaagacaat tttgggcatt atggcttagc
     2881 ggcagattac tacacacact ttacatctcc tattcgacgc tatcccgatc taatcgtcca
     2941 tcgtctgatc cgttcttaca gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc
     3001 ggaacttcct gaaattgcag atcatagttc taagatggaa cgtcgtgcag tagaaacaga
     3061 acgtgaagta gattccatga agaaagcaga attcatgatg gacaaagtgg gagaagaatt
     3121 cgatgggatc atcagctctg ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt
     3181 agaaggattg atccatatca atgaattgaa acaggactac ttccattttg ttgaaaatca
     3241 tttagctctt gtaggtgaaa gaactggtct gacttttaaa atcggacaaa aagtgcgcgt
     3301 aaaagtagtc aaagctgatc cagatgaacg agcagtggat tttgaactcg tttccgcaga
     3361 agaggtcact gcgttggaac gtccaaaagc acgcaatgga aaatctggaa aacgagaagg
     3421 aaaacaagca ggcaaacgct cagaaaaaaa tggcaagaag aacaaacgat atagtgatag
     3481 taagcaaacg ttcggtaaga aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa
     3541 gaaaaagaaa aaataaaatg agttggagga aacagctatg ccaaaaggcg agggaaaact
     3601 aattgcacaa aacaagaaag ctcgccatga ttattcgatc atcgacacga tggaagcagg
     3661 gatggtcttg caaggaacgg agatcaagtc gatacgaaac agccggatca atctgaaaga
     3721 tggatttatt cgcgtccgca acggggaagc tttcttgcat aatgttcata tcagtcctta
     3781 tgaacaagga aatattttta atcatgatcc gttgcgcacg agaaagttat tattacacaa
     3841 gaaacaaatc gttcgtcttg aaaacgaatt gaaaaacact ggaatcactg ttgttccttt
     3901 aaaagtctat attcgtaacg gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa
     3961 atcgtatgat aaacgggaag atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac
     4021 attaaaaaat ttctctagat aatttcatta tcctttttcg catattcggt gcttttgctt
     4081 ttacttaatg ataaaatttg ttagcattta atctgataaa acatgattat cacaggtttt
     4141 ttcttagatc aggagcaccg tgcatgggga aaaattcgct tcgtttttga ctgaaataaa
     4201 aaagtgtcta attttcatga aaatgttttg aattttctgt ttcatggtgg tatgataccg
     4261 tgtggttaga aaccgattgg aaaaaaccag atacgcacat gaattgattt tatgtgaatt
     4321 tcggggaaaa gaggtgaaat ggattggatg aacgctcgct aacgtttcac attggaccaa
     4381 tttggtttga tggtacagtt tgtataatgg tgttactgac ctgtttgatt gttttcttct
     4441 tagcttactt ttttacaaga aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag
     4501 aatgggtcat tgattttagt cgaggaatcg taacagataa tctgcctaga aaagaattga
     4561 gcaatttcca tttgttggca tttacattgt ttttatttgt ttttgttgca aacaacatag
     4621 gtttagtaac aaaaatcgtc ttacctaacg aaacaacact ttggaaaagc ccgacagcag
     4681 acccgtttgt tactttaact ttggcattta taatgatctt acttactcac ttattcgggg
     4741 tgaaaaagtt aggcttcaaa ggatatttcg taaattcgtt cttgaaaccc tatagtttta
     4801 tgtttccgat gaaactgatt gaagaattta caaatttatt aacacttgct ttacgtcttt
     4861 atgggaatat ttatgcgggg gaagttttgc taaccttgat tgcaaaaatg atgacaagct
     4921 taggctggtt ctcattacca ttagcaattc cattagagat ggtctggatc gcgttctcct
     4981 tgtttattgg gagcatccaa gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc
     5041 ataagattga agtagaagaa taatactcgg ttaaaaaaca aactactatt tatctatttt
     5101 aggaggaaaa caattatgaa ttatatcgca gcagcaatcg caatcatggg agcagcaatc
     5161 ggagccggtt atggtaacgg tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa
     5221 cctgaaatgt ctggtcaatt ccgtacaaca atgtttatcg gggtagctct agttgaagct
     5281 gttcctatct taggggtagt tatcgccttg attctagttt ttgccgttta attgaatcag
     5341 tattaaggca gaaacaacat cgtttgttac tgctttttta catggaaact agaaatactc
     5401 caaagaaagg aaggctcgcc tatgctgaat caattggcaa ttgcagaagt tggcaatgcg
     5461 atgttaggta atatcatcgt tgtcagcggc tcatttttga tactactggc tctcttgaag
     5521 cactttgctt ggggaccaat cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat
     5581 gatttagatt ctgcagaaca atctcgcatc aactcagcga aaatggaaca agaacgcgaa
     5641 caacaattgt tagcctctcg ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt
     5701 ggagaattaa gccgccaaaa tattttgaag gatgctcaag aagaagcagc tcgtctaaaa
     5761 agcaaagccc aagctgatat cactgtagaa cgtgattcag cgctgaactc tgtaaaagac
     5821 gacgttgcag aactctctct tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca
     5881 gaaatgcatg aatcattaat caatcaatac attgaaggtc taggttcttc aaatgaaact
     5941 agataaatat acagtaggaa gacgttacgg gaaagcttta tttgaactgg cgatcgattc
     6001 aaatcaagca gaagaaatct atcaagatct cgtaagttta cgtcaaatct atgaacaagt
     6061 acctggatta gggaatatgt taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat
     6121 catggacaag cttgtcagcg gttatgatgg gatcgttaaa aactttttag aagttgttta
     6181 tgagtataac cgaatggatg accttttgtt catgatcgaa gaatatgaac gtcgatatga
     6241 tgaacatcaa gggttactat tgggaagtgt cacaacagca gtcccacttt cagacgaaca
     6301 acgaatacaa ttagaaaaga atgttgcaaa aacaatggac tatcaatcag ttgaattaaa
     6361 acaaatcgtt gattcttcta ttattggtgg cgcgattgtt gaagccaacc atcgagtaat
     6421 cgacggaagc attcgcacgc aattagaaaa aatgcgtaat caattaaaca gatagtacaa
     6481 agaggtaggt gaatcgaatg gccatcaaag cagaagaaat tagtgccttg attaaagaac
     6541 aaattgaaaa ttatcaaaac gtgctttcag tcgaagaaat cggtaccgta acttatgtag
     6601 gtgacgggat cgctcgtgca catggtttag aaaacgcgat gagtggtgag ttgcttgaat
     6661 tttcaaacgg ctcatacgga atggcgcaaa acttagagtc aaatgatgta ggtatcatta
     6721 tccttggcga tttcgagtcc attcgtgaag gagataaagt aaaacgaaca ggtaagatca
     6781 tggaagttcc agttggagag gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa
     6841 tcgacggact aggtgaaatc gttactgaca aagctcgtcc agtagaagcg atggcaccag
     6901 gtgttatgca acgtaaatct gttaacgaac caatgcaaac agggctaaaa gcgatcgacg
     6961 cccttgtgcc aatcggacgc ggacaacgtg aattagtcat cggtgaccgt aaaacaggga
     7021 aaacttctat tgcgatcgat acgatcatca accaaaaagg tcaagatatg atctgtatct
     7081 atgtagcaat cggacaaaaa gattctacag ttcgtacaca agttgaaaca ttgaaaaaat
     7141 atggcgcaat ggattacaca atcgttgtga atgccggtgc gtctcaacca gcaccattgc
     7201 tttatatcgc accatatgct ggtactgcaa tgggtgaaga attcatgtac aacggtaaac
     7261 atgtattgat catttttgat gatctttcaa aacaagctgt tgcttatcgt gaactttcct
     7321 tgctacttcg ccgtccacca ggtcgtgaag cttatcctgg ggatgttttc tacttgcact
     7381 cacgtttatt agaacgtgca gcaaaattaa gtgatgattt aggtggcggt tcaatgactg
     7441 ccttaccatt tgttgaaaca caagctggag atatctctgc ttatatccca acaaacgtta
     7501 tttcgatcac tgatggacaa atcttcttag aaagcgactt gttctacgca ggtactcgtc
     7561 cagcggttga tgccggacta tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa
     7621 tgaaaaaagt tgctgggact ttacgcctag acttagcaag ttaccgtgaa ttagaagcat
     7681 ttacacaatt cggttctgac ttggatgcag caacacaagc aaaactaaat cgtggacgcc
     7741 gtacagtaga aatcttgaaa caaaaattgc atgcaccgct agctgttgaa aaacaagtag
     7801 tgattttgta tgctttgaca catggattct tagatagtat ccctgtagac agtattttag
     7861 acttcgaaca tgaattattc gaatatctgg ataccaatca ttcagatatc tttgaaacaa
     7921 tccgtacaac aaaagatctt ccagaagaag aagcgttgaa cagtgcgatt caggaatata
     7981 aagacatgtt cttagctaca aaaggaagta attcctctac agaagataaa ttgaaatcca
     8041 ttcaaaatgc atagtgaggt gagatgaatg ggtgcttcat taaatgaaat caagacacgt
     8101 atcgcttcaa cgaaaaaaac gagccaaatc acgcgggcaa tgcagatggt gtctgcttct
     8161 aagttgacaa aatctgaagc ttcttcacag aagtttcagg tttatgcaac aaaagtaaga
     8221 gaaatcgtta cgcatttgac tgctactcag ttaaatgata ttgcctcaga aaatcctaga
     8281 ggggatatca attacaacag catgctgctc agccgtgctg tgaagaaaac tggttatatc
     8341 gtcatcactg cagatggcgg tttagtcggt ggatataaca gctcgatatt gaaacaaacg
     8401 atgtctattt tagaagaaga ccaccaatca cccgatgatt acgtgatgat cgcaattggc
     8461 ggaactggtg cagacttctt taaagcacgc ggtatcaatc tagcatatga gctgcgcaat
     8521 ctatctgatc agccaagctt tgatgaagtc cgcaaaatcg taaatatggc aacaactatg
     8581 ttccaaaacg aagtattcga tgaattatac gtatgttata accatcatat caattcgcta
     8641 actagtcagt ttcgtgtaga aaaaatgctg ccaatctcag acttagatcc agaagaagca
     8701 acgactttcg aacaagaata tatttttgaa ccttcaaaag aagaaatctt gtctcagtta
     8761 ttgcctcagt atgctgaaag tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa
     8821 catgctgctg gtatgacagc aatgaaaaca gcaactgata atgctgctaa tatcattgat
     8881 gagctgacgg tttcttataa ccgtgcaaga caaggggcta ttacccaaga aattaccgaa
     8941 attgttgccg gagcttcggc actagaatag tattgagatt ggaggaaaaa agatgagttc
     9001 aggcaagatt gttcaagtaa tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc
     9061 cttaccagat atcaacaacg cattagttgt ttataaaaat gacgaaaata aatcaaaagt
     9121 tgttcttgaa gcagcgttag aattagggga cggagtgatc cggaccattg cgatggaatc
     9181 aacagatggt ttacaaagag gaatggaagt cattgataca ggcaaagcaa tctctgttcc
     9241 tgtaggtaaa gaaacattag gtcgcgtatt caacgtacta ggagatacga tcgatttaga
     9301 aacacctttc ccagaagatg cggaaagaag cgaaattcat aaaaaagcac cagcctttga
     9361 cgaattaagt acaagtacag aaattttgga aacagggatc aaagttatcg atttgcttgc
     9421 cccatattta aaaggtggga aagtcggact attcggtggt gccggtgttg gtaaaaccgt
     9481 actgatccaa gaactgatcc ataatatcgc ccaagaacat ggtggtattt ctgtatttac
     9541 cggtgtaggt gaacgtactc gtgaaggtaa tgacttgtat tatgaaatga aagattcagg
     9601 agttatcgaa aaaacagcca tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat
     9661 gcgtgttgct ttgactggtt tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga
     9721 tgtactgttg tttatcgaca acattttccg tttcactcaa gctggatctg aagtatcagc
     9781 cttgttagga cggatgcctt ctgcggttgg ttatcaacca acattggcaa cagaaatggg
     9841 tcaattacaa gaacgtatca catctacgaa aaaaggttcg atcacatcga tccaagcaat
     9901 ctatgtgcca gccgatgact acactgaccc ggcgccggca acagcattcg cccatttgga
     9961 tgcgacaacc aacttggaac gtaaattaac agaacaaggg atttatccag cggtagatcc
    10021 attggcttct tcttcaagtg ctttagcacc tgaaatcgtg ggggaagaac attataaagt
    10081 agcaacagaa gtccaacacg tattgcaacg atatcgtgaa ttacaagata tcattgctat
    10141 cttaggtatg gatgaattat ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt
    10201 ccaattcttc ttgtcacaaa actttaacgt ggctgaacaa tttactggtc ttccaggttc
    10261 atatgtccct gtagaagaaa cagtgaaagg attccgcgaa atcttggaag gcaaatacga
    10321 tgatcttcct gaagaagctt tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc
    10381 gaaaacacta ggctactaga aaggggtgcc ccatggatca atatttaaca gtaaatgtgg
    10441 taactcctgc cggtttggtc tatgatcacc atgcagctat cgtcgttgca cgtacgacag
    10501 ctggtgagct tgggattttg cctaaacatg caccaatcat cgttccattg acgattgatg
    10561 aagttcgtgt aaaacgaaca gattcagata cccacgtcga ttggatcgct gttaatggcg
    10621 gaatcatgga agttcgtgat aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac
    10681 gtgatatcga tgtatctcgt gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag
    10741 aagcaaaagc aaaacaagat acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag
    10801 caatcaatcg tatcaaagta tctaagcact cataaaatga gatgcggatg tttatccgtg
    10861 tttaagaaca gtaaaaagag gttatgacag aagtggtcag cttcaagaca taagaaggaa
    10921 tttacgaaaa ttgcttctca aatttttgtg aaatctcagc ttatttttcg aagaagctac
    10981 ttctggaaca ccgtatatca gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa
    11041 acaaatggaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagat
    11101 gattgaacac cgtttattt
//
LOCUS       DACIFS010000077        10884 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.077, whole genome shotgun sequence.
ACCESSION   DACIFS010000077 DACIFS010000000
VERSION     DACIFS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.077"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(13..1164)
                     /locus_tag="IYA83_002273"
     CDS             complement(13..1164)
                     /locus_tag="IYA83_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ8587687.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1318..1848)
                     /locus_tag="IYA83_002274"
     CDS             complement(1318..1848)
                     /locus_tag="IYA83_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587688.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1947..2297)
                     /locus_tag="IYA83_002275"
     CDS             complement(1947..2297)
                     /locus_tag="IYA83_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ8587689.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2454..3983)
                     /gene="ahpF"
                     /locus_tag="IYA83_002276"
     CDS             complement(2454..3983)
                     /gene="ahpF"
                     /locus_tag="IYA83_002276"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ8587690.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3993..4556)
                     /gene="ahpC"
                     /locus_tag="IYA83_002277"
     CDS             complement(3993..4556)
                     /gene="ahpC"
                     /locus_tag="IYA83_002277"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ8587691.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4700..5362)
                     /locus_tag="IYA83_002278"
     CDS             complement(4700..5362)
                     /locus_tag="IYA83_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8587692.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5546..7192
                     /locus_tag="IYA83_002279"
     CDS             5546..7192
                     /locus_tag="IYA83_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ8587693.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7183..8265
                     /gene="uxuA"
                     /locus_tag="IYA83_002280"
     CDS             7183..8265
                     /gene="uxuA"
                     /locus_tag="IYA83_002280"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ8587694.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8373..9926)
                     /locus_tag="IYA83_002281"
     CDS             complement(8373..9926)
                     /locus_tag="IYA83_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8587695.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            10074..>10884
                     /locus_tag="IYA83_002282"
     CDS             10074..>10884
                     /locus_tag="IYA83_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8587696.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEE"
BASE COUNT         3640 a         2043 c         1927 g         3274 t
ORIGIN      
        1 tttttgattt actcacactt cataaacttt ttttgtaaaa tatttgagaa caagtggaga
       61 aataagtgta cttaataaaa tcacgataac taaaggtgaa tagtactgtg gttcgatcaa
      121 ttgactttgc tgtccaattt gtaaaatgat caaagccatt tctccacgcg aaatcatacc
      181 agcaccgacc atcaatgaac tatgttgaga aaaaccggca atctgagcac caaaataacc
      241 tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata gaaataaaga gtagttgttc
      301 actaaagtca gaaaaatcaa cttctaatcc tacactgaca aaaaatactg gtataaatac
      361 tgcatatcct aaagcctcta cattgtggta tacttcttct ttaacctttg tctgactgac
      421 agctatccca gcaaaaaaag caccaatgac agagctaagc ccaacaagat ccgctacgta
      481 agacattcct aaacaaataa ctagcgatac gatgataata gaagaatttg cgtacacttt
      541 ttctgcaaat gaaagtagga aaggcgcaat ccatttgact aataagaaaa taaaaacaaa
      601 ataaattagc tgttcaatca aaacaagcgg taaagaagca ttgctagaag cttcccccgt
      661 tagaaaagat aggctgaaac ttagaactaa aacgactaaa atgtcatcta ccacagaagc
      721 acccaaaatt gttgatcctt cttttgtatt cactacgttt agttctttta atacttctac
      781 tgaaatactg acagaagttg ccgcaagaat aatcccaaag aaaatcgctt catttcccga
      841 aacttggaat gctttgcctg tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa
      901 agcaacgaac ataccaggtc ggaaatattt tttcaaaaga gataaatcac tttccagacc
      961 agctaaaaac attaataaga tcacaccaat ctccgaaaag tcatggacta gaatatcagg
     1021 tttgacccaa ttcaatccag cttggcctag caaaacacca acaagtaatt gtccaatcac
     1081 tgctgggata cctaatctcc gcgagatatg tgagccaatc gttgttgaaa tcaatatcag
     1141 gcataaaata ccaataaatt ccattctcat cattcctttg tataaaatat agcccgaact
     1201 tagcataaca tacattattg aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc
     1261 aaaagatttt tttggattgt tggtttttat acggtctatt ttaataacta aaaatgatta
     1321 acgtatagtc atttttctta ttctgatgaa attgataatc tcctggatgc ccatgaaaat
     1381 caaaataaag ccgaatattt ggaatagaag catcaaactt ttgaatggat tgaacaataa
     1441 gacgaatccg ccaatcaaca acagaatact actataaaca ctccaacttt tagcttttgt
     1501 attcatgctg aagaacaact ggaacaagct gttgatgata acagctaatc caagaagaat
     1561 cggcaggata gaaacgattg caggtgcaaa agcatagacg atcagagcta agatcaggaa
     1621 aaaaagacca ctgaacagac cgatactcca agaacctgtt ttccttttaa aacggtaagc
     1681 ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg gtgatcagat aaccgataaa
     1741 atgaaaaaca gcagaagggt ttagcacaat cgcaatccca gaaataatat aaacagcagc
     1801 tcttaataag gcgtattttt gaatattttg tttaatcgtc tcaaacatat aagaaaacct
     1861 cctttgactt attttaacgt gaactagtca ttttaccaaa ttaaatgtta caaaatgagc
     1921 gaaggagaag aatatctctg gaaaaatcaa cttctgagga tcttttccat ttttttgcct
     1981 tttgctagct catcgatcat tttatctaaa taacgaattt ctttcatcaa gttatcctcg
     2041 atgtcttcta ctcgtacacc acaaacaaca cctgtaatca aggaacgtga aggattgggg
     2101 acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt tttgtagttg atattctagt
     2161 tctgtttggg aataacccgt cagccaacaa atgatttcat ctacttcctc ttttatacga
     2221 tcttttctct ccgcttttgc tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa
     2281 atacgatttt gcgccataat cagtcacctt tctttttttc aaattattta ctacttatag
     2341 tatataagaa aaagagaaga aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa
     2401 attcttgtca ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg
     2461 cgcatcagat aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg
     2521 atttgtttgt atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt
     2581 gcgccatttt tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc
     2641 caatccgtat tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc
     2701 atctccatcg tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt
     2761 tctttcgtcg ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt
     2821 aagatttgat ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac
     2881 ccagccaaat cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct
     2941 tttcctttaa ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac
     3001 tctttttcgc ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt
     3061 tttgctttaa gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt
     3121 aaggaaacgg cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct
     3181 acttgctgca tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct
     3241 agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta
     3301 cgtgctgcat agatagctgc actactagct gctggtccac cgccaattac taatacatca
     3361 taaggttctt tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt
     3421 tgttcgatcg tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact
     3481 gccatgattt ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga
     3541 gtaatatttg gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta
     3601 tgacaagtca agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta
     3661 agactcaaag attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa
     3721 aaagaggaaa attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca
     3781 actggattga tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta
     3841 ggagacattt ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta
     3901 tccaaactaa cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt
     3961 tgtttagttt ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt
     4021 ggtttcaatg tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta
     4081 cgtacatatt gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca
     4141 tctgcattga tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc
     4201 tgagctaatc cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga
     4261 tcaccgatca tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg
     4321 tgagtaaagt gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta
     4381 ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga
     4441 tagaagcaaa gaatactcca gttccccata agatcttttt cactgacttc taaaaattct
     4501 ccttgatgat aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta
     4561 aaacctccaa taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc
     4621 aatggatatt ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag
     4681 aagaggtcgc aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt
     4741 cctgttcttt cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt
     4801 ttttatcaac taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa
     4861 gcccctgaat ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta
     4921 accagttcca aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat
     4981 ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa
     5041 tcgtttctag cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt
     5101 gctcttccaa ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg
     5161 aaacatacgt accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca
     5221 atgcttcgcg aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata
     5281 ttttccttcc aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt
     5341 gtgattgcaa gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata
     5401 acagaagagg tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata
     5461 gtaaaatttt ggttgacata gcgattttta ttgtataaaa ttcacttgaa tacaaaaact
     5521 aatatgttag tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc
     5581 tgaattatct caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa
     5641 tggacaaaaa aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca
     5701 tgcagaaatc gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg
     5761 tgaaacattc gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct
     5821 tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga
     5881 cagtctgtat tgtcatccag attgttctga acactttgat aagatgattc gacactttga
     5941 aaatcctagt ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga
     6001 tacaaataat aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc
     6061 atcacataca atgagtattc ttgctgcgct actttataaa cgttttttat ctggagcttg
     6121 gccgattgca cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc
     6181 tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca
     6241 atacttatcg aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc
     6301 gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat
     6361 tttccaaacc gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta
     6421 tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt
     6481 cttgactgat cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa
     6541 tcctttgcat acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga
     6601 ggagatgaaa gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct
     6661 cccagttgtg gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac
     6721 tgtgcgcttt tctaatccaa atattcctga tatgcctcag cgaatcgcca cggatacctc
     6781 tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa
     6841 agacccgcag acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat
     6901 tggcattgat gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagactt
     6961 tcagcataag ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa
     7021 aatggctcct attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct
     7081 tggcagaaaa atagaagaaa tgttcatcaa gatggtagca ggaccgaatg ccgtacgaaa
     7141 aacattacag gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga
     7201 tggtacggat cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc
     7261 aaagggattg taggcacatt gatggatata ccagtaggag aggtatggcc cagggagagg
     7321 atcaaagaac taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc
     7381 gtcaatgtgc atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac
     7441 tataagcaga caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt
     7501 atgcctgtat ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc
     7561 acgttagctt ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc
     7621 gaacattcca gcaatgaatt tgaattgccg ggatgggaac ctgagcgctt agctcatatc
     7681 aaatccttgc ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc
     7741 tattttctcc aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat
     7801 ccagatgatc cgccatactc gatttttgga ttacctcgaa tcgtaaagaa tcgagaagat
     7861 ttagattggt tgtgccgcat agtagacagc ccttataacg gaatcactct ttgtacaggg
     7921 agtattgcag aagatccaga aaatgatgta tatgggattt tagcagaatt ttgtcaaaga
     7981 gatcgaatcc catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat
     8041 gaggcaccgc ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg
     8101 tatgacaacg gatttaacga atacatccgt cctgatcatg gaagaatgat ctggaatgag
     8161 acaggaagac caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga
     8221 ttatgggaag caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa
     8281 ttttattatg taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta
     8341 ataagcattc ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc
     8401 gattgatcca tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc
     8461 acttttttcc agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga
     8521 gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca
     8581 ttttgtattt tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta
     8641 ctgatataaa aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag
     8701 cgatcataca aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata
     8761 actggtaaca agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt
     8821 tggacttctt tcaacacgct atgttcttga taaagcatga aagtaggaag gggcgtattt
     8881 ggttgattga agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa
     8941 agaatctttt ctttttcagg tataaacaaa tcaaatacag tacaaaagga ctccaccatt
     9001 tctcttgaat aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag
     9061 cttgtttctt ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg
     9121 cgttgttcgc gacaaattgg cagattaggt cgcaaaatat ctacagaggg atcaataaaa
     9181 aagtcaatat attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga
     9241 aaattctctt tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt
     9301 cggtcaagta cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct
     9361 tcatcataaa attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca
     9421 tattgaattt gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata
     9481 gacaaaccgt acaatgctaa aaatgcatta atctttttca ttttcgtata caaataagag
     9541 gcactatagt ttgtttgctg acttaaactg ataatcgaat ttgtctgaca gctgaacaaa
     9601 gaagtcaaga tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag
     9661 cagtaagccg gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata
     9721 tgcagttctg ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa
     9781 aaatttgatt cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga
     9841 tgttcagata ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagagaaac
     9901 gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac
     9961 ttgaaaatat tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag
    10021 aatctctact atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat
    10081 attcaaccat cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga
    10141 cagtccacac agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca
    10201 atcgaattac ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa
    10261 aaggacaaac agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac
    10321 tacaatcagc tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga
    10381 acactttgac tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg
    10441 acttgttaaa accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc
    10501 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag
    10561 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
    10621 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
    10681 atacagaaac tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa
    10741 aaccagatgt agacccagaa gaaaaaccag atgtgactcc agaaccagat acggattctg
    10801 gaaaccaaac agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa
    10861 aaccagaggt agccccagaa gaaa
//
LOCUS       DACIFS010000078         9954 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.078, whole genome shotgun sequence.
ACCESSION   DACIFS010000078 DACIFS010000000
VERSION     DACIFS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.078"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(1..657)
                     /locus_tag="IYA83_002283"
                     /pseudo
     CDS             complement(1..657)
                     /locus_tag="IYA83_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(662..1429)
                     /locus_tag="IYA83_002284"
     CDS             complement(662..1429)
                     /locus_tag="IYA83_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8587697.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="IYA83_002285"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="IYA83_002285"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ8587698.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="IYA83_002286"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="IYA83_002286"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ8587699.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="IYA83_002287"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="IYA83_002287"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ8587700.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="IYA83_002288"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="IYA83_002288"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ8587701.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="IYA83_002289"
     CDS             4828..5145
                     /locus_tag="IYA83_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ8587702.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="IYA83_002290"
     CDS             5145..6851
                     /locus_tag="IYA83_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8587703.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="IYA83_002291"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="IYA83_002291"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ8587704.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="IYA83_002292"
     CDS             8422..8694
                     /locus_tag="IYA83_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ8587705.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="IYA83_002293"
     CDS             8715..9614
                     /locus_tag="IYA83_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ8587706.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3142 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcg
//
LOCUS       DACIFS010000079         9928 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.079, whole genome shotgun sequence.
ACCESSION   DACIFS010000079 DACIFS010000000
VERSION     DACIFS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.079"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            4..360
                     /locus_tag="IYA83_002294"
     CDS             4..360
                     /locus_tag="IYA83_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587707.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IYA83_002295"
     CDS             376..726
                     /locus_tag="IYA83_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ8587708.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IYA83_002296"
     CDS             755..1534
                     /locus_tag="IYA83_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ8587709.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IYA83_002297"
     CDS             1545..2222
                     /locus_tag="IYA83_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ8587710.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IYA83_002298"
     CDS             2241..3347
                     /locus_tag="IYA83_002298"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ8587711.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IYA83_002299"
     CDS             3337..4428
                     /locus_tag="IYA83_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ8587712.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IYA83_002300"
     CDS             4441..5181
                     /locus_tag="IYA83_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ8587713.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="IYA83_002301"
     CDS             5469..7409
                     /locus_tag="IYA83_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ8587714.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="IYA83_002302"
     CDS             7496..9418
                     /locus_tag="IYA83_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ8587715.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9698..>9928)
                     /locus_tag="IYA83_002303"
     CDS             complement(9698..>9928)
                     /locus_tag="IYA83_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ8587716.1"
                     /translation="LIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQA
                     VGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3238 a         1648 c         2205 g         2837 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aactaaatac aaaaaaagag gatgtggcca aagttgtggc acatcctttt attttgagtt
     9541 tcttataatt ttttatagtt gcttacgatg tcttgactac tggtaaacgg tgttcaagag
     9601 acaatttgtc ggcaataagg cacaattttg taaaatatgg aaagctattt ttttaacatt
     9661 gtttcttagt gcttgaaaaa tcattttgaa tacttcttta tacgatcgat gtacgttgat
     9721 ttaagtctaa aacttttctt ttgattcctt ctctgacttt tctgaattcc gttaatattt
     9781 cttcttcgct cccgacagct tgtgcaggat cttttaggtc ccagtgaagg tgttcgtgat
     9841 tgtttggaac ggcagggcac ttgtctttgg catcaccaca aagtgtaatg atcaagtctg
     9901 cctgagtaaa gtaatctcta tcaatcaa
//
LOCUS       DACIFS010000080         9862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.080, whole genome shotgun sequence.
ACCESSION   DACIFS010000080 DACIFS010000000
VERSION     DACIFS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.080"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(251..1099)
                     /locus_tag="IYA83_002304"
     CDS             complement(251..1099)
                     /locus_tag="IYA83_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ8587717.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1262..2164
                     /gene="gnd"
                     /locus_tag="IYA83_002305"
     CDS             1262..2164
                     /gene="gnd"
                     /locus_tag="IYA83_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ8587718.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2166..3167
                     /locus_tag="IYA83_002306"
     CDS             2166..3167
                     /locus_tag="IYA83_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ8587719.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3164..3448
                     /locus_tag="IYA83_002307"
     CDS             3164..3448
                     /locus_tag="IYA83_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ8587720.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3481..4278
                     /locus_tag="IYA83_002308"
     CDS             3481..4278
                     /locus_tag="IYA83_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ8587721.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4292..5125
                     /locus_tag="IYA83_002309"
     CDS             4292..5125
                     /locus_tag="IYA83_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8587722.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5143..6000
                     /locus_tag="IYA83_002310"
     CDS             5143..6000
                     /locus_tag="IYA83_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ8587723.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6022..6531
                     /locus_tag="IYA83_002311"
     CDS             6022..6531
                     /locus_tag="IYA83_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587724.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6568..6969
                     /locus_tag="IYA83_002312"
     CDS             6568..6969
                     /locus_tag="IYA83_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587725.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7081..7434
                     /locus_tag="IYA83_002313"
     CDS             7081..7434
                     /locus_tag="IYA83_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587726.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7468..7911
                     /locus_tag="IYA83_002314"
     CDS             7468..7911
                     /locus_tag="IYA83_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ8587727.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7928..9013
                     /gene="uxuA"
                     /locus_tag="IYA83_002315"
     CDS             7928..9013
                     /gene="uxuA"
                     /locus_tag="IYA83_002315"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ8587728.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9006..9719
                     /locus_tag="IYA83_002316"
     CDS             9006..9719
                     /locus_tag="IYA83_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ8587729.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3292 a         1558 c         2171 g         2841 t
ORIGIN      
        1 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
       61 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
      121 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
      181 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
      241 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
      301 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
      361 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
      421 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
      481 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
      541 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
      601 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
      661 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
      721 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
      781 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
      841 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
      901 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
      961 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
     1021 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
     1081 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
     1141 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
     1201 taaaatctat ttacaaaaaa attttttgta taatgagggt ataagaaatg gaggcgctat
     1261 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
     1321 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
     1381 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
     1441 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
     1501 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
     1561 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
     1621 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
     1681 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
     1741 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
     1801 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
     1861 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
     1921 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
     1981 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
     2041 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
     2101 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
     2161 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     2221 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     2281 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     2341 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     2401 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     2461 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
     2521 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     2581 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     2641 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     2701 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     2761 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     2821 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     2881 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     2941 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     3001 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     3061 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     3121 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     3181 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     3241 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     3301 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     3361 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     3421 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     3481 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     3541 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     3601 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     3661 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     3721 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     3781 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     3841 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     3901 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
     3961 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     4021 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     4081 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     4141 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     4201 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     4261 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     4321 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     4381 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     4441 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     4501 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     4561 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     4621 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     4681 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     4741 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     4801 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     4861 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     4921 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     4981 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     5041 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     5101 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     5161 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     5221 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     5281 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     5341 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     5401 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     5461 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     5521 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     5581 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     5641 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     5701 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     5761 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     5821 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     5881 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     5941 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     6001 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     6061 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     6121 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     6181 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     6241 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     6301 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     6361 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     6421 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     6481 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     6541 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     6601 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     6661 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     6721 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     6781 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     6841 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     6901 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     6961 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     7021 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     7081 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     7141 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     7201 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     7261 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     7321 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     7381 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     7441 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     7501 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     7561 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     7621 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     7681 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     7741 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     7801 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     7861 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     7921 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     7981 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     8041 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     8101 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     8161 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     8221 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     8281 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     8341 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     8401 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     8461 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     8521 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     8581 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     8641 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     8701 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     8761 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     8821 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     8881 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     8941 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     9001 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     9061 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     9121 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     9181 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     9241 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     9301 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     9361 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     9421 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     9481 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     9541 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     9601 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     9661 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     9721 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     9781 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     9841 aaacacaaaa cgcatctact gt
//
LOCUS       DACIFS010000081         8907 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.081, whole genome shotgun sequence.
ACCESSION   DACIFS010000081 DACIFS010000000
VERSION     DACIFS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.081"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(51..1517)
                     /locus_tag="IYA83_002317"
     CDS             complement(51..1517)
                     /locus_tag="IYA83_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ8587730.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IYA83_002318"
     CDS             1904..2500
                     /locus_tag="IYA83_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ8587731.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IYA83_002319"
     CDS             complement(2558..3040)
                     /locus_tag="IYA83_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587732.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IYA83_002320"
     CDS             complement(3146..3343)
                     /locus_tag="IYA83_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587733.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IYA83_002321"
     CDS             3638..4396
                     /locus_tag="IYA83_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ8587734.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IYA83_002322"
     CDS             4456..4884
                     /locus_tag="IYA83_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587735.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IYA83_002323"
     tRNA            4930..5002
                     /locus_tag="IYA83_002323"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IYA83_002324"
     CDS             complement(5152..5523)
                     /locus_tag="IYA83_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ8587736.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IYA83_002325"
     CDS             5738..5923
                     /locus_tag="IYA83_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587737.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IYA83_002326"
     CDS             complement(6006..6194)
                     /locus_tag="IYA83_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587738.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IYA83_002327"
     CDS             complement(6218..6481)
                     /locus_tag="IYA83_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587739.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IYA83_002328"
     CDS             6639..6896
                     /locus_tag="IYA83_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ8587740.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IYA83_002329"
     CDS             complement(6967..7158)
                     /locus_tag="IYA83_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587741.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IYA83_002330"
     CDS             complement(7299..7619)
                     /locus_tag="IYA83_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587742.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IYA83_002331"
     CDS             complement(7642..8076)
                     /locus_tag="IYA83_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ8587743.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8907
                     /locus_tag="IYA83_002332"
     CDS             8411..>8907
                     /locus_tag="IYA83_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ8587744.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIG"
BASE COUNT         2835 a         1780 c         1423 g         2869 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tactagaatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggacaacaat taacaatccc
     8641 aacagaaggc tctgcacaag cagctgctga acctcaagca gccgttcaag aagcaccagt
     8701 ccaagctgaa cctgttcaag ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac
     8761 acaagcagcg cctgttgctg aaacacaacc tgctcctgct gtaacagaaa ctgctgctac
     8821 tccagcaagt acaagctctg caaaagaatg gatcgcacaa aaagaatcaa gcggttctta
     8881 cacagcaacg aacggacgtt acatcgg
//
LOCUS       DACIFS010000082         8735 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.082, whole genome shotgun sequence.
ACCESSION   DACIFS010000082 DACIFS010000000
VERSION     DACIFS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.082"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(474..1061)
                     /locus_tag="IYA83_002333"
     CDS             complement(474..1061)
                     /locus_tag="IYA83_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587745.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1236..2558
                     /locus_tag="IYA83_002334"
     CDS             1236..2558
                     /locus_tag="IYA83_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8587746.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2713..3489
                     /locus_tag="IYA83_002335"
     CDS             2713..3489
                     /locus_tag="IYA83_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ8587747.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3482..4051
                     /gene="rnmV"
                     /locus_tag="IYA83_002336"
     CDS             3482..4051
                     /gene="rnmV"
                     /locus_tag="IYA83_002336"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ8587748.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4044..4928
                     /gene="rsmA"
                     /locus_tag="IYA83_002337"
     CDS             4044..4928
                     /gene="rsmA"
                     /locus_tag="IYA83_002337"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ8587749.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5379..6008
                     /locus_tag="IYA83_002338"
     CDS             5379..6008
                     /locus_tag="IYA83_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587750.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYA83_002339"
     CDS             complement(6132..7241)
                     /gene="ugpC"
                     /locus_tag="IYA83_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ8587751.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7450..7872
                     /gene="mgsA"
                     /locus_tag="IYA83_002340"
     CDS             7450..7872
                     /gene="mgsA"
                     /locus_tag="IYA83_002340"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ8587752.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            8195..8731
                     /locus_tag="IYA83_002341"
     CDS             8195..8731
                     /locus_tag="IYA83_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ8587753.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2766 a         1543 c         1790 g         2636 t
ORIGIN      
        1 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
       61 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
      121 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
      181 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
      241 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
      301 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
      361 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
      421 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
      481 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
      541 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
      601 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
      661 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
      721 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
      781 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
      841 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
      901 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
      961 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
     1021 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
     1081 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
     1141 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
     1201 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
     1261 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
     1321 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
     1381 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
     1441 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
     1501 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
     1561 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
     1621 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
     1681 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
     1741 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
     1801 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
     1861 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
     1921 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
     1981 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
     2041 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
     2101 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
     2161 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
     2221 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
     2281 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
     2341 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
     2401 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
     2461 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
     2521 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
     2581 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
     2641 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
     2701 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
     2761 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
     2821 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
     2881 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
     2941 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
     3001 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
     3061 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
     3121 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
     3181 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
     3241 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
     3301 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
     3361 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
     3421 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
     3481 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
     3541 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
     3601 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
     3661 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
     3721 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
     3781 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
     3841 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
     3901 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
     3961 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
     4021 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
     4081 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
     4141 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
     4201 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
     4261 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
     4321 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
     4381 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
     4441 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
     4501 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
     4561 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
     4621 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
     4681 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
     4741 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
     4801 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
     4861 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
     4921 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
     4981 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
     5041 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
     5101 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
     5161 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
     5221 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
     5281 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
     5341 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
     5401 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
     5461 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
     5521 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
     5581 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
     5641 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
     5701 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
     5761 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
     5821 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
     5881 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
     5941 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
     6001 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
     6061 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
     6121 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
     6181 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
     6241 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
     6301 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
     6361 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
     6421 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
     6481 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
     6541 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
     6601 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
     6661 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
     6721 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
     6781 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
     6841 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
     6901 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
     6961 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
     7021 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
     7081 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
     7141 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
     7201 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
     7261 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
     7321 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
     7381 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
     7441 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
     7501 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
     7561 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
     7621 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
     7681 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
     7741 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
     7801 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
     7861 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
     7921 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
     7981 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
     8041 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
     8101 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
     8161 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
     8221 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
     8281 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
     8341 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
     8401 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
     8461 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
     8521 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
     8581 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
     8641 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
     8701 tttaagaagt ttagcgatcc acgctaaata aaaaa
//
LOCUS       DACIFS010000083         8733 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.083, whole genome shotgun sequence.
ACCESSION   DACIFS010000083 DACIFS010000000
VERSION     DACIFS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.083"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            2..74
                     /locus_tag="IYA83_002342"
     tRNA            2..74
                     /locus_tag="IYA83_002342"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IYA83_002343"
     tRNA            78..150
                     /locus_tag="IYA83_002343"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IYA83_002344"
     tRNA            155..225
                     /locus_tag="IYA83_002344"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IYA83_002345"
     tRNA            238..311
                     /locus_tag="IYA83_002345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IYA83_002346"
     tRNA            323..394
                     /locus_tag="IYA83_002346"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IYA83_002347"
     CDS             660..1853
                     /locus_tag="IYA83_002347"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ8587754.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IYA83_002348"
     CDS             1983..3455
                     /locus_tag="IYA83_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ8587755.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IYA83_002349"
     CDS             3538..4326
                     /locus_tag="IYA83_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ8587756.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IYA83_002350"
     CDS             complement(4393..5061)
                     /locus_tag="IYA83_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ8587757.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IYA83_002351"
     CDS             5176..6888
                     /locus_tag="IYA83_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587758.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IYA83_002352"
     CDS             6891..8663
                     /locus_tag="IYA83_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587759.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2623 a         1487 c         1945 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc aga
//
LOCUS       DACIFS010000084         8603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.084, whole genome shotgun sequence.
ACCESSION   DACIFS010000084 DACIFS010000000
VERSION     DACIFS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.084"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYA83_002353"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYA83_002353"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ8587760.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IYA83_002354"
     CDS             complement(1098..2201)
                     /locus_tag="IYA83_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ8587761.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IYA83_002355"
     CDS             complement(2291..3226)
                     /locus_tag="IYA83_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ8587762.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IYA83_002356"
     CDS             3528..5462
                     /locus_tag="IYA83_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ8587763.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IYA83_002357"
     CDS             5919..6335
                     /locus_tag="IYA83_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ8587764.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IYA83_002358"
     CDS             6349..6915
                     /locus_tag="IYA83_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ8587765.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IYA83_002359"
     CDS             6938..7924
                     /locus_tag="IYA83_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ8587766.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IYA83_002360"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="IYA83_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ8587767.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1658 c         1750 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DACIFS010000085         8453 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.085, whole genome shotgun sequence.
ACCESSION   DACIFS010000085 DACIFS010000000
VERSION     DACIFS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.085"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..1329)
                     /locus_tag="IYA83_002361"
     CDS             complement(<1..1329)
                     /locus_tag="IYA83_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ8587768.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIKPVVLVNFNILKNISKFL"
     gene            complement(1470..2072)
                     /locus_tag="IYA83_002362"
     CDS             complement(1470..2072)
                     /locus_tag="IYA83_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ8587769.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2317..2523
                     /locus_tag="IYA83_002363"
     CDS             2317..2523
                     /locus_tag="IYA83_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587770.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2835..3554
                     /locus_tag="IYA83_002364"
     CDS             2835..3554
                     /locus_tag="IYA83_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ8587771.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3635..4318)
                     /locus_tag="IYA83_002365"
     CDS             complement(3635..4318)
                     /locus_tag="IYA83_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ8587772.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4536..6722
                     /locus_tag="IYA83_002366"
     CDS             4536..6722
                     /locus_tag="IYA83_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ8587773.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7068..7478
                     /locus_tag="IYA83_002367"
     CDS             7068..7478
                     /locus_tag="IYA83_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ8587774.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7492..7848
                     /locus_tag="IYA83_002368"
     CDS             7492..7848
                     /locus_tag="IYA83_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ8587775.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3106 a         1382 c         1583 g         2382 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttttatttg
       61 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
      121 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
      181 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
      241 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
      301 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
      361 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
      421 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
      481 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
      541 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
      601 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
      661 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
      721 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
      781 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
      841 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
      901 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
      961 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
     1021 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
     1081 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
     1141 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
     1201 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
     1261 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
     1321 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
     1381 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
     1441 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
     1501 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
     1561 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
     1621 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
     1681 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
     1741 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
     1801 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
     1861 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
     1921 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
     1981 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
     2041 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
     2101 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
     2161 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
     2221 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
     2281 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
     2341 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
     2401 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
     2461 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
     2521 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
     2581 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
     2641 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
     2701 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
     2761 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
     2821 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
     2881 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
     2941 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
     3001 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
     3061 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
     3121 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
     3181 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
     3241 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
     3301 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
     3361 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
     3421 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
     3481 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
     3541 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
     3601 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
     3661 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
     3721 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
     3781 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
     3841 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
     3901 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
     3961 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
     4021 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
     4081 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
     4141 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
     4201 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
     4261 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
     4321 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
     4381 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
     4441 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
     4501 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
     4561 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
     4621 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
     4681 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
     4741 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
     4801 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
     4861 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
     4921 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
     4981 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
     5041 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
     5101 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
     5161 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
     5221 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
     5281 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
     5341 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
     5401 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
     5461 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
     5521 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
     5581 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
     5641 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
     5701 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
     5761 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
     5821 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
     5881 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
     5941 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
     6001 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
     6061 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
     6121 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
     6181 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
     6241 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
     6301 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
     6361 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
     6421 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
     6481 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
     6541 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
     6601 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
     6661 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
     6721 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
     6781 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
     6841 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
     6901 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
     6961 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
     7021 aaagaaatta ctgtagaatt ggattataat taaaaaaggg tgagaaaatg ataaaagtat
     7081 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
     7141 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
     7201 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
     7261 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
     7321 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
     7381 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
     7441 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
     7501 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
     7561 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
     7621 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
     7681 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
     7741 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
     7801 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
     7861 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
     7921 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
     7981 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     8041 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     8101 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     8161 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     8221 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     8281 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     8341 gcagccgaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     8401 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa aca
//
LOCUS       DACIFS010000086         8423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.086, whole genome shotgun sequence.
ACCESSION   DACIFS010000086 DACIFS010000000
VERSION     DACIFS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.086"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            471..2107
                     /locus_tag="IYA83_002369"
                     /pseudo
     CDS             471..2107
                     /locus_tag="IYA83_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYA83_002370"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="IYA83_002370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ8587776.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="IYA83_002371"
     CDS             complement(3183..3818)
                     /locus_tag="IYA83_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ8587777.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYA83_002372"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="IYA83_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ8587778.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="IYA83_002373"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="IYA83_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6416..>8423
                     /locus_tag="IYA83_002374"
     CDS             6416..>8423
                     /locus_tag="IYA83_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ8587779.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDSFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1548 c         1292 g         2798 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacagcttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaa
//
LOCUS       DACIFS010000087         8228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.087, whole genome shotgun sequence.
ACCESSION   DACIFS010000087 DACIFS010000000
VERSION     DACIFS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.087"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IYA83_002375"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IYA83_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ8587780.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IYA83_002376"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IYA83_002376"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ8587781.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IYA83_002377"
     CDS             2591..3376
                     /locus_tag="IYA83_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8587782.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IYA83_002378"
     CDS             3389..4240
                     /locus_tag="IYA83_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ8587783.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IYA83_002379"
     CDS             complement(4364..4816)
                     /locus_tag="IYA83_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8587784.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IYA83_002380"
     CDS             complement(5281..5649)
                     /locus_tag="IYA83_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ8587785.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IYA83_002381"
     CDS             5799..6281
                     /locus_tag="IYA83_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ8587786.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IYA83_002382"
     CDS             6318..7328
                     /locus_tag="IYA83_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ8587787.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IYA83_002383"
     CDS             complement(7443..8156)
                     /locus_tag="IYA83_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587788.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACIFS010000088         8046 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.088, whole genome shotgun sequence.
ACCESSION   DACIFS010000088 DACIFS010000000
VERSION     DACIFS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.088"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(98..1228)
                     /locus_tag="IYA83_002384"
     CDS             complement(98..1228)
                     /locus_tag="IYA83_002384"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ8587789.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IYA83_002385"
     CDS             complement(1877..3079)
                     /locus_tag="IYA83_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8587790.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IYA83_002386"
     CDS             complement(3139..3342)
                     /locus_tag="IYA83_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ8587791.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IYA83_002387"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IYA83_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IYA83_002388"
     CDS             4879..5691
                     /locus_tag="IYA83_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8587792.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="IYA83_002389"
     CDS             complement(6715..6867)
                     /locus_tag="IYA83_002389"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587793.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IYA83_002390"
     CDS             complement(7058..7936)
                     /locus_tag="IYA83_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ8587794.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
BASE COUNT         2457 a         1647 c         1254 g         2688 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagta
//
LOCUS       DACIFS010000089         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.091, whole genome shotgun sequence.
ACCESSION   DACIFS010000089 DACIFS010000000
VERSION     DACIFS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.091"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYA83_002391"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYA83_002391"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ8587795.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IYA83_002392"
     CDS             complement(2032..3273)
                     /locus_tag="IYA83_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ8587796.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IYA83_002393"
     CDS             complement(3291..4214)
                     /locus_tag="IYA83_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ8587797.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IYA83_002394"
     CDS             complement(4435..6927)
                     /locus_tag="IYA83_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ8587798.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IYA83_002395"
     CDS             complement(6934..7401)
                     /locus_tag="IYA83_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ8587799.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IYA83_002396"
     tRNA            complement(7537..7610)
                     /locus_tag="IYA83_002396"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IYA83_002397"
     tRNA            complement(7668..7741)
                     /locus_tag="IYA83_002397"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACIFS010000090         7699 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.092, whole genome shotgun sequence.
ACCESSION   DACIFS010000090 DACIFS010000000
VERSION     DACIFS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.092"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            544..1107
                     /locus_tag="IYA83_002398"
     CDS             544..1107
                     /locus_tag="IYA83_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587800.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IYA83_002399"
     CDS             complement(1386..1673)
                     /locus_tag="IYA83_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ8587801.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IYA83_002400"
     CDS             complement(1663..1992)
                     /locus_tag="IYA83_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8587802.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IYA83_002401"
     CDS             2297..2626
                     /locus_tag="IYA83_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587803.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYA83_002402"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYA83_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ8587804.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IYA83_002403"
     CDS             complement(3551..4462)
                     /locus_tag="IYA83_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ8587805.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IYA83_002404"
     CDS             complement(4890..5498)
                     /locus_tag="IYA83_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ8587806.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYA83_002405"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYA83_002405"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ8587807.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IYA83_002406"
     CDS             complement(6528..7496)
                     /locus_tag="IYA83_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ8587808.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2350 a         1532 c         1281 g         2536 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatt
//
LOCUS       DACIFS010000091         7627 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.094, whole genome shotgun sequence.
ACCESSION   DACIFS010000091 DACIFS010000000
VERSION     DACIFS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.094"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(14..706)
                     /locus_tag="IYA83_002407"
     CDS             complement(14..706)
                     /locus_tag="IYA83_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ8587809.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IYA83_002408"
                     /pseudo
     CDS             791..967
                     /locus_tag="IYA83_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IYA83_002409"
     CDS             complement(1143..1712)
                     /locus_tag="IYA83_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ8587810.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IYA83_002410"
     CDS             complement(1786..2109)
                     /locus_tag="IYA83_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587811.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IYA83_002411"
     CDS             complement(2263..3075)
                     /locus_tag="IYA83_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587812.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IYA83_002412"
     CDS             3429..4277
                     /locus_tag="IYA83_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ8587813.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IYA83_002413"
     CDS             4422..5363
                     /locus_tag="IYA83_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ8587814.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IYA83_002414"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IYA83_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2472 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aaccttt
//
LOCUS       DACIFS010000092         7597 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.095, whole genome shotgun sequence.
ACCESSION   DACIFS010000092 DACIFS010000000
VERSION     DACIFS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.095"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(135..425)
                     /locus_tag="IYA83_002415"
     CDS             complement(135..425)
                     /locus_tag="IYA83_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587815.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IYA83_002416"
     CDS             complement(543..803)
                     /locus_tag="IYA83_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587816.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IYA83_002417"
     CDS             complement(940..1353)
                     /locus_tag="IYA83_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ8587817.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IYA83_002418"
     CDS             complement(1809..2294)
                     /locus_tag="IYA83_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587818.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IYA83_002419"
     CDS             complement(2433..2648)
                     /locus_tag="IYA83_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ8587819.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IYA83_002420"
     CDS             2872..3672
                     /locus_tag="IYA83_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8587820.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IYA83_002421"
     CDS             3691..5559
                     /locus_tag="IYA83_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8587821.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IYA83_002422"
     CDS             5552..6043
                     /locus_tag="IYA83_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ8587822.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IYA83_002423"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IYA83_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ8587823.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IYA83_002424"
     CDS             6603..>7597
                     /locus_tag="IYA83_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ8587824.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACIFS010000093         7398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.096, whole genome shotgun sequence.
ACCESSION   DACIFS010000093 DACIFS010000000
VERSION     DACIFS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.096"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            69..152
                     /locus_tag="IYA83_002425"
     CDS             69..152
                     /locus_tag="IYA83_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ8587825.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="IYA83_002426"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="IYA83_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ8587826.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="IYA83_002427"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="IYA83_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1607..2272
                     /locus_tag="IYA83_002428"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="IYA83_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="IYA83_002429"
     CDS             2308..2553
                     /locus_tag="IYA83_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ8587827.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="IYA83_002430"
     CDS             2656..3525
                     /locus_tag="IYA83_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ8587828.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="IYA83_002431"
     CDS             3506..4240
                     /locus_tag="IYA83_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8587829.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5181
                     /locus_tag="IYA83_002432"
     CDS             4273..5181
                     /locus_tag="IYA83_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ8587830.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5178..5720
                     /gene="sat4"
                     /locus_tag="IYA83_002433"
     CDS             5178..5720
                     /gene="sat4"
                     /locus_tag="IYA83_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ8587831.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5813..6607
                     /locus_tag="IYA83_002434"
                     /note="aph(3')-IIIa"
     CDS             5813..6607
                     /locus_tag="IYA83_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ8587832.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6715..6903)
                     /locus_tag="IYA83_002435"
                     /pseudo
     CDS             complement(6715..6903)
                     /locus_tag="IYA83_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6883..>7398
                     /locus_tag="IYA83_002436"
     CDS             6883..>7398
                     /locus_tag="IYA83_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8587833.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         2592 a         1117 c         1555 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gtattcgaat tgtgaccctt gaggggtcac gcgcaaatat taatatacct
     4381 aaagatgaat ttcaggatta tgatattaca tattttgtaa gtgatataga accgtttata
     4441 tctaatgatg actggcttaa tcaatttggg aatataataa tgatgcaaaa gccggaggat
     4501 atggaattat tcccacctga agaaaaggga ttttcctatc ttatgctatt tgatgattac
     4561 aataaaattg atcttacctt attgcccttg gaagagttag ataattacct aaagggcgat
     4621 aaattaataa aggttctaat tgataaagat tgtagaatta aaagggacat agttccgact
     4681 gatatagatt atcatgtaag aaagccaagc gcaagggagt atgatgattg ctgcaatgaa
     4741 ttttggaatg taacacctta tgttattaaa ggattgtgcc gtaaggaaat tttatttgct
     4801 attgatcatt ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc
     4861 ggcatcgaaa caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat
     4921 atatccgagg atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac
     4981 atatgggaag cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg
     5041 gaaaggcttc attatgccta tccggagtat gataggaata taacaaaata taccagggac
     5101 atgtataaaa aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata
     5161 gaagagaggc gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata
     5221 aacccagcga accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt
     5281 ggacctttac agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg
     5341 atgaagagga tgaggaggca gattgccttg aatatattga caatactgat aagataatat
     5401 atctttacta ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc
     5461 ggtacgctta tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca
     5521 gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc
     5581 ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa
     5641 tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt
     5701 tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat
     5761 tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa
     5821 aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac
     5881 ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt
     5941 aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat
     6001 gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga
     6061 tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga
     6121 agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca
     6181 ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgat tagccgaatt
     6241 ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac
     6301 tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga
     6361 acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt
     6421 aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt
     6481 ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga
     6541 cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt
     6601 gttttagtac ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta
     6661 atcttttctg aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa
     6721 gttcgctaga tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag
     6781 tatggctttt ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca
     6841 taggatatac tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga
     6901 gtaacctcta ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca
     6961 cagcgattta atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc
     7021 ggaataccaa ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt
     7081 ccgccggtaa tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta
     7141 aattgccatg gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa
     7201 tctattttga agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg
     7261 tacagcacat ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact
     7321 gcaatctgca ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg
     7381 gaaagcagaa tgatagag
//
LOCUS       DACIFS010000094         7158 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.097, whole genome shotgun sequence.
ACCESSION   DACIFS010000094 DACIFS010000000
VERSION     DACIFS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.097"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(560..1600)
                     /locus_tag="IYA83_002437"
     CDS             complement(560..1600)
                     /locus_tag="IYA83_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ8587834.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IYA83_002438"
     CDS             complement(1949..2275)
                     /locus_tag="IYA83_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587835.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IYA83_002439"
     CDS             complement(2262..3050)
                     /locus_tag="IYA83_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ8587836.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="IYA83_002440"
     CDS             complement(3418..3708)
                     /locus_tag="IYA83_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ8587837.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="IYA83_002441"
     CDS             complement(3844..4023)
                     /locus_tag="IYA83_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587838.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="IYA83_002442"
     CDS             complement(4020..4370)
                     /locus_tag="IYA83_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587839.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="IYA83_002443"
     CDS             complement(4363..5688)
                     /locus_tag="IYA83_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ8587840.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="IYA83_002444"
     CDS             6138..6752
                     /locus_tag="IYA83_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587841.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..>7158)
                     /locus_tag="IYA83_002445"
     CDS             complement(6917..>7158)
                     /locus_tag="IYA83_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587842.1"
                     /translation="SFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGIS
                     ITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2188 a         1292 c         1062 g         2616 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagct cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attctttcac attatacaaa catatgttcg atattgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tattctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctat
//
LOCUS       DACIFS010000095         7035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.098, whole genome shotgun sequence.
ACCESSION   DACIFS010000095 DACIFS010000000
VERSION     DACIFS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.098"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            541..2133
                     /locus_tag="IYA83_002446"
     CDS             541..2133
                     /locus_tag="IYA83_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ8587843.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2146..2886
                     /locus_tag="IYA83_002447"
     CDS             2146..2886
                     /locus_tag="IYA83_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ8587844.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            3054..4403
                     /locus_tag="IYA83_002448"
     CDS             3054..4403
                     /locus_tag="IYA83_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8587845.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4531..5121)
                     /locus_tag="IYA83_002449"
     CDS             complement(4531..5121)
                     /locus_tag="IYA83_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ8587846.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            5268..6620
                     /gene="brnQ"
                     /locus_tag="IYA83_002450"
     CDS             5268..6620
                     /gene="brnQ"
                     /locus_tag="IYA83_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ8587847.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         2099 a         1286 c         1428 g         2222 t
ORIGIN      
        1 aattttctgt tctagttctt tttttattgt attacataca tttttgtgta taattatact
       61 atcgtttgca atggatagat gattcacatt ttgattgttt tttcggtaaa agtaaagatt
      121 tctatattta tcagtatttt ttcaaaaacc agcagttata gattgacaaa gcctctcgag
      181 tatcgtaaac tttttttgta taaataactg aatacgagat gcctcaatca atgaggtaga
      241 ggtcgcgaga atcattaacc ctgtggagtg aagcaaacat ggagaagcag gaatagggag
      301 gatcgccgaa atgtgcactt ttttgcttgg agtgtatgtt gggacgcaag ttaagagctt
      361 gcggactgtc ttagcagtga tgctaagttg cgctatgttt tgtgagaggt ttatcacctt
      421 atggaatttt agcggccttt ttatatctat aaatcggtcg ctttttattt tttcagataa
      481 agctttaccg catttcatct agaccgctcg tgtatcatat aatcagaaag ggagaaaaaa
      541 atgaaaaaaa atatggtttc agttgccgta ctatttgctt ttgtcttctc actattcagt
      601 gtgggatctc tggcaaatgc agatgaaaaa acaccgagcg atgagtttcg cgtaggtatg
      661 gaggctgggt atgcaccctt caactggtct caacaaacag atgagaataa tgccttgcca
      721 attcaaggcc aaaatagtta cgccggcggt tatgatgttc aaatcgctaa aaaagtagca
      781 gatggtttag ggaaaaaatt agtcatcgtc caaacgaaat gggatggtct ggctcctgcc
      841 cttcaatcag gcaagatcga cgcgatcatc gccggaatga gccctactgc tgaacgacga
      901 aaagaaattg attttaccga tccttattac gaatcacaat tagtagtagt agttcaaaaa
      961 gatagcaaat atgcaaaagc aaaaagttta gcagatcttt ctggagcaaa gatcactgct
     1021 cagttaaata cattccacta tactgtgatc gatcagatac cagatgtgca gaaacaacaa
     1081 gcaatggata atttctcagc aatgagaaca gctttagctt ctggaatgat cgatggttat
     1141 gtcagcgaac gtccggaagg tgtaacggca actagtgtga acaaagactt ggaaatgctt
     1201 gaattcagcg gtgcagatgg cttccagaca aacccggaag atgttcagat ttctgttggt
     1261 atgcgtaaag gcgacccgga tatccaaaaa gtaaaccaga ttctttctgg catctctaac
     1321 gatgaacgaa ccaaaattat ggaccaagca gtcaaagatc agcctgctgc aactgatagt
     1381 gaagaaaaac ctggcatcat ttctgatttc actacgattt ggaaacaata cggcaatatg
     1441 ttcttacgag gtgctggact taccttgttc atcgcattga tcgggacagt tgtcggtact
     1501 acacttggat tactgattgg tgtcttccgt tcggtacctg aatcaggaaa tgcactgact
     1561 cgcttcttcc aaaaaatcgg aaatgcactt ctttctatct atgtcgaagt attccgcgga
     1621 acaccgatga tggtacaagc aatggtcatc ttctacggtc tggcactcgc ttttggcatt
     1681 tcattgaacc gaacgattgc cgctttgttt attgtctctc tcaatactgg agcttacatg
     1741 acagagatcg ttcgtggggg gatttttgca gtagatgaag ggcagttcga agctgcgcaa
     1801 gccattggta tgacccatgg ccaaacgatg agaaaagttg ttattccaca agttttacgg
     1861 aatatcttgc cagcaacagg aaatgagttt gttatcaata tcaaagatac agcagtttta
     1921 agtgtaatcg gcgtagctga tttgttcttc caaggaaact ctgcttccgg tgcgaatttc
     1981 caattcttcc aaacatttac gatcgtcggg atcatctact tagtcatgac ctttacgatc
     2041 accagaatcc tgcgggcggt agaaaagaaa atcgatggtc cttcagctta taccaagctt
     2101 gaagaaatcg acaattcaaa tttgcaggaa taaggaggaa gaatcatgaa tgaaaaaatt
     2161 attgaaatca aacatctaag aaaaagcttc ggtgaaaatg aagtattgaa agatatcagc
     2221 gtaacagtca ataaaggtga agttgtgacg atcatcggtt cttccggttc tggtaaatct
     2281 actttcttgc gctgtatcaa tttactggaa aaaccaacag gcggcgagat catttataat
     2341 ggagaaaatg tattagctcc taaatataat ttacctaaat atcgaacgaa tctaggtatg
     2401 gttttccaat ccttcaacct gttcaacaat atgaatgtct tggaaaactg tatgtctggt
     2461 caaacaactg tcctgaaacg agataaagaa actgccaaaa aagtagcaat cgaaaatctg
     2521 gaaaaagttg gtatggatcg ttatatcgaa gccaaaccag cacaattatc tggtggacag
     2581 aaacagcgtg tcgcaattgc tcgggcatta tcaatgaatc cagatgtatt gctattcgac
     2641 gaacctactt cggcgttaga ccctgaaatg gttggcgaag tgcttaatac gatcaaagac
     2701 ttagcacata ctggcttgac aatgcttatc gtgacgcatg agatggaatt tgcaaaagat
     2761 gtatctgacc gtgtcatttt tatggataaa ggtgtgattg cagaagaagg tacaccagaa
     2821 gatatctttg tccatccgaa agaagaacgt acaaaagaat tcctttcacg tattttaaat
     2881 gcttaggatc aatagctgat tgggacaaag agctgcggtg tttaccgcag ttctttttta
     2941 tgaattactc ataaaaggta aatttctgaa aattttggaa tcttagttgt gtttttcaga
     3001 aaactctctt aatatggaag taattaaact tttttagtta ggatgattta aaaatgaatg
     3061 aagagcaaga actgcagcgt ggactgaaaa atagacatgt ccaactgatt tctattggcg
     3121 gagcgattgg aacggggttg tttttaggtt ccggaaagtc tatccagcta gctggccctt
     3181 cgatattgct cgcctatctt attactggat gcatctgttt tttcattatg cgtgcgttag
     3241 gcgaacttct gttatcaaac accaataacc attcttttct ggattttgtt gcagaatatc
     3301 ttggcaaaaa agcggctttc atcactggct ggacgtattg gttctgctgg gtatcgattg
     3361 caatggccga tttaacagcc atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc
     3421 agtggcttcc tgaactgatt gcattaggtc ttcttctttg cttgaatatg gtggctgtct
     3481 cattgtttgg tgagttagag ttttggtttg cattgatcaa agttgtagcg atcattgcct
     3541 ttatcctcgt aggagcttat atgatcctca cccactatac gacagatatt ggcacagcca
     3601 gtatcacgaa tctatggtct catggcggat tctttccaac tggtgcaaaa ggatttatct
     3661 tagctttcca aatggtaacc tttgcttttg ctgggataga attagttgga ttagtcgctg
     3721 gagaaacaga aaatcctgaa aaagtgttgc cggaagctat caataatatt cctatcagga
     3781 tcatcttatt ttacttagga tctctttttg tcattatggc aatctatcca tggaacagct
     3841 tgaatccaga taacagtcca tttgtagaag tattttcgga aattgggatc acgattgctg
     3901 cttccttgat caatttagtc gtattaagtg cagcagcttc tgcctgcaac agtgcaatat
     3961 atagtacagg acgtatgctt cgttctttag cgcaagaagg cagtgcccct aaaaaattca
     4021 aaaagttaac aacacatcat gtgccaggga atgctttgac attttctact atcgtcattt
     4081 tcatttctgt tatcttgaat tacgtgatgc ctagtgaagt attcacacta gtaagcagta
     4141 ttgccactac ttgtttcttg tttatctgga gtatgcttgt ctacacgcac atgaaatatc
     4201 gtaaaagtat cctcggcaaa aaagctcatt catttaaaat gccgttgtac ccatttagta
     4261 attatctggt atttctttac atggcctttg tgtgtgtcgt cttgttccta ggaaaagata
     4321 cgagaatcgc tttattacta acacctgtgt ggttcttgct tctattacta atctatcata
     4381 tgaaatatga acgtaagaag taagaaagta gttgtgacag aagtggtcag cttcgagaaa
     4441 taagaaggaa tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttcttg
     4501 gagataaaag cttctgtccc agcctctttt ctatttcccc ggaaacaacc gctgataata
     4561 ggcatctgct tttttatagg aaaaaatcca aataccagat acaaaaaaac gtccgaataa
     4621 cccgatcaac ggtacaaaaa agacaccggc aatcaagcat aagttaatca tcatggagat
     4681 aagacttcga cggtcctttg acaaaactct cacgacatgg gaatctggtt tatttgcttt
     4741 catgatttgc ctcatcaaga taccaaaaga aaaactccac atcgtgtata ctaagacata
     4801 agccatctca ggccaaaaag cttcagggtt acgcccgacc caactagtaa cagaaggaca
     4861 taaagtcagc caaaagagca aaaagatatt cgaccagacc actcggtaat taattcgttc
     4921 tgccatctga aatatacgat gatggttcac ccagatcacc gcaacaaata taaagctgat
     4981 ggtataagct aataatgtat ttcggatgac ccataaagaa tgaaagctag atgtattagg
     5041 gatcttgatc tctaatacaa taactctcaa aacaatggct accactccat cagtaaatgc
     5101 ttctaatctc gactttgtca cgatgattcc tcctcagatg attcagcgct tttattttat
     5161 cacttacgtt attaaataac catcgtttta tatagggaat tttctgaaaa ttgttgcata
     5221 tttttcgaaa ttttgttact tttaaaaaaa ccgtaaagga gttatgtatg acaaaaaaat
     5281 tatcatttaa agactatctt tttataggct ccatgctttt cggtctattt tttggtgctg
     5341 gaaatcttat ctttcccgtt catttgggtc aagaagcagg cgctgctacc ttttgggcga
     5401 atctaggttt cttggttacc ggtatcggtc ttcccttctt aggggtgatt gccattggtg
     5461 tatcgaaaag ctcaggtgta tatgaattag caaaacggat aaatcaaaca tatgcgatgg
     5521 tttttaccat cttgctttac ttagttatcg gaccgttttt tgctttacct cgccttgcaa
     5581 ccacatcttt cgagattggt ttagcaccat ttatcgatcc tagcagacag acgctatatc
     5641 tagcaatttt cagtattgta ttttttgtgc tggcttggtg gttttcaaga aaaccgacaa
     5701 agatattaga ttatgttgga aagttcctga atcctgcatt tttggtttta ttaggtgctt
     5761 tgcttctcct tgcttttttg aatccactag gttctgtaaa tcaagcgcct attcagccaa
     5821 attatcaaga gcatgctttt ttcacagggt ttactcaagg atataacaca ttagatgcgc
     5881 ttgctgcttt agcatttggg atcatcattg tcacaacaat ccaaaacatg ggtgtaacaa
     5941 aacctgctga aatcgctaaa gacacgatca aatctggtgc gatcagcatt atattgatgg
     6001 gaatcattta tactttgctc gcttatttag gtgctatgag cttaggtagc tttgctctaa
     6061 gtgaaaatgg cgggatcacg ctggctcaga ttgctgatca ttatttaggt acttatggca
     6121 gtatcttatt agcatttatc gttatattag cttgcttgaa gaccggtatt ggtttgatta
     6181 cagcttttgc agaaacattc acagatttgt ttccgcaaag aaattacgca ttctttgttg
     6241 cacttgctag tatccttcct tgtttagcgg caaacgtagg cttgacgaat atcattcaat
     6301 tttcgttgcc tgttttgatg ttcatctatc cattagcaat gacactgatt ttactagtgt
     6361 tagttggtcc tttgtttaag cagcgtacgg ctgtttatcg aatgactact tattttacat
     6421 tgattgcttc gatttttgat gggttgaacg cttgcccaga aactatcaaa caaacaccta
     6481 ttgttcaaaa tcttttatat gttgcggaaa gctacttgcc gtttttcaaa ttggggatgg
     6541 ggtggatcgt accagcagtt atcggttttg ttatcggact gatttggagt ttcgctaaga
     6601 aagaagaagt agcagattga taaaagaggt tgtgaaagag acgttcagcc ttgagcaata
     6661 aaaagtaatt ttgaaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga
     6721 aactgacttt tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat
     6781 taagaaatat tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga
     6841 gaaactggct tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa
     6901 acaaaacggc aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag
     6961 aagctgatca aacaacattt cttttataat atagatttta gacatatata tgatataaaa
     7021 atagttttcg caaaa
//
LOCUS       DACIFS010000096         6584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.099, whole genome shotgun sequence.
ACCESSION   DACIFS010000096 DACIFS010000000
VERSION     DACIFS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.099"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(22..969)
                     /locus_tag="IYA83_002451"
     CDS             complement(22..969)
                     /locus_tag="IYA83_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ8587848.1"
                     /translation="MSERISYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1147..1950)
                     /locus_tag="IYA83_002452"
     CDS             complement(1147..1950)
                     /locus_tag="IYA83_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ8587849.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2096..3010
                     /locus_tag="IYA83_002453"
     CDS             2096..3010
                     /locus_tag="IYA83_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ8587850.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3058..3528
                     /locus_tag="IYA83_002454"
     CDS             3058..3528
                     /locus_tag="IYA83_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ8587851.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3613..4221
                     /locus_tag="IYA83_002455"
     CDS             3613..4221
                     /locus_tag="IYA83_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ8587852.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4355..4555
                     /locus_tag="IYA83_002456"
     CDS             4355..4555
                     /locus_tag="IYA83_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ8587853.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            5309..6535
                     /locus_tag="IYA83_002457"
     CDS             5309..6535
                     /locus_tag="IYA83_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ8587854.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2228 a         1000 c         1317 g         2039 t
ORIGIN      
        1 tgaaaatatt gttttcagct attaatatcc tgcagacaat tttacttgat tggtttgaag
       61 ctcatgggtg gcaaagtagc ttttcagatt ggttaaaaaa atagccatta atttcttttg
      121 aaaatcaggt gtcaaacctg caatgtggga agtaatcagt acattgtcca ttttccaaag
      181 aggatgatct tgtggaagag gctcttcctc aaaaacgtct aatgctgcaa aggaaagttg
      241 ctggttattt aaagcctgca ccagatcttg tgtcttaaca gaagcaccac ggccaacatt
      301 gatgaataca gattttggat caaagtgttc aaacatatgc tgatcaaaga gcccagttgt
      361 ttcctccgtt agaggtaaga tattgactac gaaatctact tcaggaagaa cttcctttag
      421 ttgatttaaa gaaaaagtct tttccattcc ttctgctgga tgaccggatg tattgattcc
      481 atagcagttc atgtctaatg ctttgcattt ttttgcaatc atttttccaa tatggccagt
      541 tccaatgatt agtatttttt tcccttggat agattggtaa tggaccgatg aacctaacca
      601 tttcatgtca ctctgtgcct tttgtgcttg aaaaagacca cgagaatacc caaggatgat
      661 tcctaagaca tgctccgtaa tcgattcact gtggatgcca ctaccatttg aaagtaaaat
      721 atgtttcttt gaaaaagtct caaggggtag ctgatcaaca ccagctgaga tagattggac
      781 ccatttcaag gtagaagagg ctaatagttt ttcttggtag tctgagttcc agcccaaact
      841 gatctctaca ttttgtagat cctctggttg taattctgtt ttaaagatat aaccaggcgc
      901 tatagtatgg atttcttcga taaattcatc acgaaatgga cgatctgcat aactgattct
      961 ttctgacata gttaatttcc cgcctttctt tactctaagt gtagcgcata caagaagaac
     1021 tttgaagcgc cttgcttgtt ttttgagaaa gaaatatttt tcaaataaaa aaggggctgt
     1081 gacaaaaaaa tgtcacaacc tctaaaaggg tctattgaag tagaaataac gagttgtctt
     1141 tatttcttaa ttgatcgata tttttgccca tcctttgaca gcttgagcaa aaataaagat
     1201 tcctgcaaaa gcaatgaagg cgctagtgtc aaaagaagga agaaagacca tcaagattgt
     1261 tgcaattata gtaggcaatg aaattgaata gatcaatgtt tttaaacaat caagaaaagt
     1321 agcttttgtt agtcgaaagc gtgcatagag aaaagcagca atgttggtaa atagcaaagt
     1381 gatcagcaga ttcagaaaac taggataaat agaaaatata aatgtcagga cttttatcca
     1441 aagtggtata gatgcttcac ttaaaaaaga acggagctgc ttacctgtta agttttttaa
     1501 agattcattg gtgtagggaa gttctaattg gttattgttc aacaaagcag aagtcgtgcc
     1561 tgtattagga agagctatga taagtttgtt ttttaacatc ccgacactaa caaaattccc
     1621 catcaaatca gtagagatgt cctgttctgt tcgttttccc tctggatcaa atgtgaagat
     1681 aatggagttt gtttggtaaa tgaatccttc tttttcttcg gtttgaagct gcccgttttg
     1741 gattgtaaaa tcaggaatgc gagtagcaat cttttgccca tccaattgaa tgttgtttaa
     1801 gacttgaaaa acttgaaaag agattggcaa agccataatt atactcaaaa ctaaaagata
     1861 gacaatggct ttccaaaatg ggacatttct cgcctccttt aggtctttga atcgtataaa
     1921 ggaactgata attaattgtt ttgttgtcat aagtactcct catctagtag tgtcatcact
     1981 tgcaattcta gcgaagtatc aagtgaaata caagtgcttt aaggaaacag ctgaatgatt
     2041 ggtgaaatat caaaaaaatg ttattcttgt ctaaagacta tccaatataa ataaaatgga
     2101 ggaaggaata gtgcctacac agccacagag atcatcaaaa aaactatttg gagtagtggt
     2161 tttgaatgcg gtgatcactt tatcagaatt tattggaggg attatgtcag ggagtttgtc
     2221 tcttgtttca gatgctttcc ataaccttag tgatacattg gcaattattt tttcttacgg
     2281 ggcacaaaaa atggcccgaa aagaagcaaa taaaaaacgt acgtatggct accagcgttt
     2341 agaaatattg agtgctttca tcaactcatt catattgatc atattatcct tgtttttaac
     2401 cgttgaagca ttcaagcggt tcacttcacc agaaaagata aatagtcatt tgatgctaac
     2461 agtagcagtt attggattgc tcgcgaattt gttctcagta ttattattac gacaagaagc
     2521 agatgaaagt ttgaatataa aatctagtta tttgcatttg ttaagtgata cactgtcttc
     2581 ggtaagtgtg attatcggag caattttgat ccgttttttc gatatctact ggatcgatcc
     2641 agttattaca ctgatcattt cgatatatat attgatagaa gcgataatgg tgatcaaaaa
     2701 agctgccgct attttgatac aaagtgcacc tgctatcgac tatgaaaaaa tggaacaaga
     2761 aattgaagca atcgatgggg taaaggatgt tcatcatgtc catatttggc agtatagcga
     2821 aaagataatt attttcgacg gacatattga ttttgaagat cagctactaa gcgagattga
     2881 aaagatatat cctcgtataa tttctttatt aaaattaaaa tatggtatta cacatgtgac
     2941 gatccaagca gagacaaagg caaaggatca aaaaaaatta atttttttaa ataaagatga
     3001 aagttattga aatatagagt atgataaata agaattttta ataaggtagg ttggctcatg
     3061 aagcagtatc gacagttaaa aggatattta gaagcaaaag gatattggga gatactagtc
     3121 tatctctttt ttggtggttt ggctacgatt gtaaattttg ttagttttgc tctcgcacgc
     3181 caatattttg atttaagtat ggccttatcc aattctattt cgtggttttg ttctgtattg
     3241 tttgcttttg taacgaacaa actttgggta ttccattcaa aatcaccgaa ttttacacat
     3301 gcacttattg aatgcggtaa gttcttcttc taccgtatcc tttcttacgg attggacatg
     3361 ggggcaatgg tattgttgat caatgttatg aacagcaatg agtatgtggc gaaaatcatc
     3421 actcaaatta ttgttatcct tgcgaactat attttcagca agttgtttat ttttaaagaa
     3481 actgaggttt ttgaagaaga agtaggtcaa agtgaaaaag acgactaggc ataacaattg
     3541 aagaatttcc ttctctttgt tactctttgt aatgtagaca ttattaagaa aacattgagg
     3601 aggaattttt ttatgactta cacattacca gatctacctt atgcatatga cgcattggaa
     3661 ccttatattg acgaagaaac gatgcatctg catcatgata agcatcacaa tacttatgtg
     3721 acgaatttaa attcagcaat tgagaaatac ccagaattag gcgaaaaaac aatagaagaa
     3781 ttattatctg atatggacgc tattccaaca gatatcaaga cagctgtacg taacaatggt
     3841 ggcggacatg ctaaccattc atttttctgg gaaatcatgg caccaaatgc tggtggcgaa
     3901 cctacaggag aaataaaaga agcgattaat gaagcttttg gtgatttttc ttcttttaaa
     3961 gaagaattca aaaaagcagc cgctggacga tttggttctg gatgggcttg gcttgtaatg
     4021 gaaaatggaa aattagctat tacctctact gcaaatcaag attctccatt gatggaagga
     4081 aagacaccaa ttctaggttt ggacgtttgg gaacatgctt attatttgaa atataaaaat
     4141 gtccgaccag attatattgc agcattctgg aatgttatta attgggatga agtaaacaaa
     4201 cgtttcgaag cagcaaaata accatttcct ccgtcatatc agtcttaaaa ataatttgga
     4261 aaataaaaga gcgaaagctt gctagcgttt tctaaattgg gtataatgtt cttgcaaggt
     4321 tacttgcaag aacttttttg gaggatatac atttatggaa cacggaacgg ttaaatggtt
     4381 caacaatgaa aaaggatttg ggtttattac agtagacggc ggagatgacg tatttgttca
     4441 tttctcagca attcaaggag acggcttcaa gagcctagaa gaaggtcaag aagtagaatt
     4501 cacgattgtt gaaggtgcac gcggacctca agctgctgaa gtgactaaat tataataatt
     4561 tgttgaataa agacacgact tcttagagag tcgtgtcttt ttgtctattt atgaagtttt
     4621 tagatttttt ggctaagtaa atgcatttgt tcacttgaca tgtcctaact aattatcaag
     4681 caaggaacat tttcttcgtt agtaatggat tttaaagaaa tttcagtttt tggatgttga
     4741 ataaaagacc atttgtgaaa ccactagtat acatactcat taccatgtca attctattac
     4801 tttaaaagaa aatcgtttta ctttgaagag gagcatatct atccattaaa agatgatggt
     4861 aatgcaatca ctagttcgag tcattttaag agccatgcag tgagctggaa ataaaacgaa
     4921 tagaagatgg gtttaacaaa aggaccagtc ataggaagaa attgtcgtga aatcgaatga
     4981 taatctgaac ggtaggcaaa gactgttaaa aagatgggaa aggtatgtgc gatcatttta
     5041 ctgcacgatt atttggaaaa taccttgttg ttcgcttttt ctggaaaaaa tacataaaaa
     5101 aagataaaac gattgcgaac tgcgtgtatt cggtctttcg atctttttca cggtatggta
     5161 tgatagttaa gaattttgag aaattttaaa aaataataat cgttacacaa aaatgttttt
     5221 taaagttttc ttatgattaa aaagtgaatt tgctgattga acagttatat gagacaatat
     5281 tttagaaaat gtgaaacaga ggtgtattat gaagcgatgg caaaaagttc tattaacctt
     5341 gttaggggta ttcgttttgc tgattgccgg attatcagca tacggaatca gatttatggg
     5401 agaagcgagc caaacagtaa acaaaatttc taaaaactct aatcgaatat cctccaaacg
     5461 ttctgagaga gtaagcattg atgacaaaga acctttttcg gttttgctat taggtttaga
     5521 taccggtgga ttaggaagaa cggaacaagg acgttctgat acgatgatgg tagtaacagt
     5581 taatccgaaa caaaaaaaat caacgatcat cagtttggac cgagatattt atacgaatat
     5641 cgtaggttat ggaacggtag ataaattaaa tcatgcctac gcatttggtg gtgtcgaaat
     5701 ggccatggat tcaattgagc aactgctcga tattcctatt gatcattatg taacgatcaa
     5761 tttggatggc atggaagatt tgatcaatgc ggtcggtggc atcaaagtga ataataaaat
     5821 tgactttact ttggatggtg tccatgtacc agtaggtgaa caaacattga atggagaaaa
     5881 aggtttagcc tattctcgaa tgcgacacga agatccagaa ggcgatatcg gtcgacaacg
     5941 tcgtcagcgg gaagtagtga cgaaaatcgt caataaagtt ttaagtttag atggcgtaag
     6001 caattataga aaaattttaa aagcagttga gaacaatgtg acgacggact tagattggga
     6061 tgacatgctt gatgtagcga cgaactacac cccggcgttt gaaacaatca aacaagacca
     6121 gttgcaaggc gaaggacaaa tgatcaatga tatctactat caaattttag gaaaaaatga
     6181 tcttctttcg atccaaaatg aactgaagaa acaattaaat ataaaaacaa gcgatacatt
     6241 acctaatttg aaaaatgaga atgcttcaat gatgttcttt gatgattcgg aagatggcga
     6301 ttcgacaaac ggtgatggat cgaatagtga agggtacacc gattattctt cctattcaga
     6361 aaacacatat gatcagtacc aagatccgaa tacctattat cagcaaacag aagatacaag
     6421 tcagtactat acaccaagtc aagatactca aagttatgat ccttacgctg gacaggaaca
     6481 aaatggttat ggttatgacc agaataatga gtatggaaca acaagtgggt actagaaaaa
     6541 aatagtttgg ttaataaaga gtgccaccta aattagacaa aaaa
//
LOCUS       DACIFS010000097         6173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.101, whole genome shotgun sequence.
ACCESSION   DACIFS010000097 DACIFS010000000
VERSION     DACIFS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.101"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            725..811
                     /locus_tag="IYA83_002458"
     CDS             725..811
                     /locus_tag="IYA83_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ8587855.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IYA83_002459"
     CDS             complement(932..1621)
                     /locus_tag="IYA83_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587856.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IYA83_002460"
     CDS             complement(1628..1906)
                     /locus_tag="IYA83_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587857.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IYA83_002461"
     CDS             complement(1866..2780)
                     /locus_tag="IYA83_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ8587858.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IYA83_002462"
     CDS             complement(2762..3142)
                     /locus_tag="IYA83_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ8587859.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IYA83_002463"
     CDS             complement(3592..3879)
                     /locus_tag="IYA83_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ8587860.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IYA83_002464"
     CDS             complement(3899..4123)
                     /locus_tag="IYA83_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ8587861.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IYA83_002465"
     CDS             complement(4763..5296)
                     /locus_tag="IYA83_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ8587862.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IYA83_002466"
     CDS             complement(5317..6159)
                     /locus_tag="IYA83_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ8587863.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACIFS010000098         6121 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.102, whole genome shotgun sequence.
ACCESSION   DACIFS010000098 DACIFS010000000
VERSION     DACIFS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.102"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(261..731)
                     /locus_tag="IYA83_002467"
     CDS             complement(261..731)
                     /locus_tag="IYA83_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ8587864.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1061..2701
                     /locus_tag="IYA83_002468"
     CDS             1061..2701
                     /locus_tag="IYA83_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ8587865.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2855..4177
                     /locus_tag="IYA83_002469"
     CDS             2855..4177
                     /locus_tag="IYA83_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ8587866.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4245..4679)
                     /locus_tag="IYA83_002470"
     CDS             complement(4245..4679)
                     /locus_tag="IYA83_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587867.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4744..5580)
                     /locus_tag="IYA83_002471"
     CDS             complement(4744..5580)
                     /locus_tag="IYA83_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ8587868.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1834 a         1211 c         1137 g         1939 t
ORIGIN      
        1 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
       61 tcagaacgtt gacttcacag tctcttcctt aaacggtgtt caaaaacttg catctgcggt
      121 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
      181 tgatgcaggg caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt
      241 ttacgaaata ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc
      301 gtttcttgcc attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt
      361 cctaattcaa aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca
      421 gacacatgat tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc
      481 tcatgatgaa cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc
      541 tcataaatat cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct
      601 actgtcagtt cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct
      661 tctactacgc cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga
      721 acttctttca ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac
      781 ataggtgact cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat
      841 tccatctatc ccttattctt ctacttttct ttctctagca tataagagat acgcatagta
      901 ttcaagcaag gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct
      961 atattacata taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact
     1021 tctatacttc gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt
     1081 attaaataat ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata
     1141 tggaatcact ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc
     1201 ttatggtcaa tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct
     1261 attcaatacg aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat
     1321 gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt
     1381 gaaaccgcaa gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt
     1441 gaagaacgcg gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat
     1501 cttaggtatg ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt
     1561 ctatactgct gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg
     1621 tactaataat gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt
     1681 agaaggcgaa acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt
     1741 cccagaattg ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga
     1801 aaaatacgaa gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt
     1861 cctagcaggt gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat
     1921 tttgacattt ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat
     1981 gatccgtcaa ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca
     2041 aggtttattg tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa
     2101 acgctcagat ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat
     2161 ccatccgaca atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt
     2221 gaaagaaatg gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa
     2281 attggcagaa gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg
     2341 aacaggtatc cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc
     2401 aaataatgct ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg
     2461 agtaaatgct gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac
     2521 aaaaccagga gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat
     2581 tgctgaaact gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga
     2641 tatcaaagaa gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta
     2701 agatcgttat agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg
     2761 actgcggcaa aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt
     2821 tcatatgata cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac
     2881 tgcagttgag gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt
     2941 gccactttat ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc
     3001 gacgaatatg attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat
     3061 cgggacaaaa atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt
     3121 cgcttctgct gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga
     3181 aaactttgtc agcaatatgg gcttttggga cttttatatt gctgccttga ttactggtag
     3241 catccttggt atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc
     3301 acttatttca atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa
     3361 tggttttgca aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc
     3421 cggagccgtt cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat
     3481 catttcccgc ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc
     3541 attagttgct cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga
     3601 aaaaggcgat cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg
     3661 tgttggtttg atggtatcat tgactttctt catcttagga caaattggta actattttgt
     3721 tcctaaagtc catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa
     3781 cctattacca gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa
     3841 cttgacagca gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc
     3901 ttctgctttg acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt
     3961 tgtttcagga tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc
     4021 tggcttatgc aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa
     4081 tcggatggaa cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt
     4141 gatcatctct ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat
     4201 catcaagtat cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa
     4261 aatctttttt gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct
     4321 actctgtatt ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct
     4381 tctacgccag tcatcaaagc ctttccgatg cctctttttt cagcatattt agacacagca
     4441 agtgccataa tattgaacat tggatcgcta aaaatcgatt cataggactc cgcttgtaca
     4501 tagccagctg cttttttaga agtaggatct tcataaacca agaaaaaatt tttctctggc
     4561 tccttcagca gtttcttcat ttgtctcacc gtaacggtta atggtatatc ataccctaat
     4621 tgatccgcat tgatttcacg tagtgcttct gcatcttcta aacgcatttc ccgtaacatc
     4681 caaatcacca gccaatttct gattttttaa aatgaatttt tttatcatag tatactcttt
     4741 ttttcattta gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa
     4801 tgtttgtaag atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac
     4861 ttgaaaaata aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa
     4921 acctccaata acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa
     4981 gctacttgtt aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag
     5041 agaatatagt gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt
     5101 gactggtcca ccaccaatac ctaaaaaact agctaaaaag cccagaaccc ccccacatat
     5161 aaggtaccaa gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa
     5221 cgcaaaaacc aaggtcaata ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt
     5281 ttcaaataac tgtaaaaaaa cttcgaatac aatgttgccg actactcctc caacaacagc
     5341 tccactagat acccaaccga caatctgcca attcagtgat ttcccgcttg atacttgtcg
     5401 cacagtcgaa acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc
     5461 aacagaatgg gcgccgataa gatcaagaat cggtttgatc aacacgcctc cacccatccc
     5521 ggaaatagcg ccaactgtgt tcgccagaac gatcaccaca aaataaatca gaccaatcat
     5581 cttgtatttc ctttctcttg tttatatctt ttagcgattg ttactctttt cttttattat
     5641 acagtaaaag aaacaaacga ggcttcgaaa gcttttctac tttttaagga aatttagtgc
     5701 taaacgaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     5761 aaatagctcc tcataatttt cgtttggttg aacttattac cgcagatgca agcttttgaa
     5821 caccgtttaa gaaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt
     5881 caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga
     5941 gaacaccgtt taaggaagag actgtgaagt caacgttttg acctgattat tagagaaaca
     6001 aatggcaaaa tagttcttca tatttcgccg tttttcgagc gaatgccgag gggcagctga
     6061 ttgaacatcg tttatttgga aaaagggatt gtgacaagac gtttgtcaca atcccttttt
     6121 t
//
LOCUS       DACIFS010000099         6111 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.103, whole genome shotgun sequence.
ACCESSION   DACIFS010000099 DACIFS010000000
VERSION     DACIFS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.103"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(38..634)
                     /locus_tag="IYA83_002472"
     CDS             complement(38..634)
                     /locus_tag="IYA83_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ8587869.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="IYA83_002473"
     CDS             984..1328
                     /locus_tag="IYA83_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ8587870.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="IYA83_002474"
     CDS             1329..1748
                     /locus_tag="IYA83_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ8587871.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="IYA83_002475"
     CDS             complement(1840..2190)
                     /locus_tag="IYA83_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587872.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="IYA83_002476"
     CDS             complement(2156..3487)
                     /locus_tag="IYA83_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ8587873.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="IYA83_002477"
     CDS             3742..4308
                     /locus_tag="IYA83_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ8587874.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="IYA83_002478"
     tRNA            4453..4540
                     /locus_tag="IYA83_002478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /locus_tag="IYA83_002479"
     CDS             4876..5850
                     /locus_tag="IYA83_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ8587875.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         2219 a         1027 c         1130 g         1735 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt a
//
LOCUS       DACIFS010000100         6068 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.104, whole genome shotgun sequence.
ACCESSION   DACIFS010000100 DACIFS010000000
VERSION     DACIFS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.104"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            832..1566
                     /locus_tag="IYA83_002480"
     CDS             832..1566
                     /locus_tag="IYA83_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ8587876.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IYA83_002481"
     CDS             1582..2751
                     /locus_tag="IYA83_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ8587877.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IYA83_002482"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IYA83_002482"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ8587878.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IYA83_002483"
     CDS             3841..4767
                     /locus_tag="IYA83_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ8587879.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IYA83_002484"
     CDS             4786..6036
                     /locus_tag="IYA83_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8587880.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACIFS010000101         6059 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.105, whole genome shotgun sequence.
ACCESSION   DACIFS010000101 DACIFS010000000
VERSION     DACIFS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.105"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            293..2524
                     /gene="pflB"
                     /locus_tag="IYA83_002485"
     CDS             293..2524
                     /gene="pflB"
                     /locus_tag="IYA83_002485"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ8587881.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2768..3529
                     /gene="pflA"
                     /locus_tag="IYA83_002486"
     CDS             2768..3529
                     /gene="pflA"
                     /locus_tag="IYA83_002486"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ8587882.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3614..4540
                     /locus_tag="IYA83_002487"
     CDS             3614..4540
                     /locus_tag="IYA83_002487"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ8587883.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4722..5615
                     /locus_tag="IYA83_002488"
     CDS             4722..5615
                     /locus_tag="IYA83_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ8587884.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5749..5874
                     /locus_tag="IYA83_002489"
     CDS             5749..5874
                     /locus_tag="IYA83_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587885.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         2143 a         1022 c         1195 g         1699 t
ORIGIN      
        1 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
       61 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
      121 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
      181 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
      241 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
      301 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
      361 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
      421 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
      481 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
      541 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
      601 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
      661 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
      721 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
      781 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
      841 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
      901 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
      961 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
     1021 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
     1081 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
     1141 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
     1201 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
     1261 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
     1321 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
     1381 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
     1441 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
     1501 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
     1561 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
     1621 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
     1681 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
     1741 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
     1801 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
     1861 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
     1921 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
     1981 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
     2041 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
     2101 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
     2161 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
     2221 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
     2281 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
     2341 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
     2401 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
     2461 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
     2521 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
     2581 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
     2641 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
     2701 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
     2761 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
     2821 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
     2881 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
     2941 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
     3001 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
     3061 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
     3121 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
     3181 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
     3241 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
     3301 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
     3361 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
     3421 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
     3481 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
     3541 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
     3601 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
     3661 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
     3721 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
     3781 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
     3841 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
     3901 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
     3961 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
     4021 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
     4081 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
     4141 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
     4201 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
     4261 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
     4321 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
     4381 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
     4441 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
     4501 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
     4561 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
     4621 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
     4681 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
     4741 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
     4801 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
     4861 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
     4921 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
     4981 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
     5041 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
     5101 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
     5161 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
     5221 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
     5281 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
     5341 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
     5401 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
     5461 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
     5521 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
     5581 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
     5641 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
     5701 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
     5761 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
     5821 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
     5881 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
     5941 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
     6001 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaat
//
LOCUS       DACIFS010000102         6000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.106, whole genome shotgun sequence.
ACCESSION   DACIFS010000102 DACIFS010000000
VERSION     DACIFS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.106"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(65..934)
                     /locus_tag="IYA83_002490"
     CDS             complement(65..934)
                     /locus_tag="IYA83_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ8587886.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1226..2836)
                     /locus_tag="IYA83_002491"
     CDS             complement(1226..2836)
                     /locus_tag="IYA83_002491"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ8587887.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3058..3303)
                     /locus_tag="IYA83_002492"
     CDS             complement(3058..3303)
                     /locus_tag="IYA83_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587888.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFGTHKGLKK"
     gene            complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="IYA83_002493"
     CDS             complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="IYA83_002493"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ8587889.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(5397..5789)
                     /locus_tag="IYA83_002494"
     CDS             complement(5397..5789)
                     /locus_tag="IYA83_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8587890.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1760 a         1281 c          988 g         1971 t
ORIGIN      
        1 agaatggaga gcgacttcaa aagtcgttct ccattcctat atatcgtatc tgttatgtgg
       61 atgcttacgc tttgttagct gaaccgaacc agtcgatatg agatttagca tcttcaacga
      121 ttgctgcttt acctggagct aataatttac gagggtcgaa tcctttacct tctttatctt
      181 taccagcttc gatatattta cgagtagctg cagcaaatga taattggaat tcagtgttaa
      241 catttacttt tgagataccc attgagatag ctttttcgat ttgatcttga ggaatacctg
      301 atccgccatg taatactaaa ggcatgtctg gacctacagc atcagcaatt gcttgtaagt
      361 ggtcaaaagc taatcctttc cagttttctg gatattgacc atggatgtta ccgataccgc
      421 aagctaagta gtcgatacct gtagcaacca tttgtttgca ttcttcaata tcagctaatt
      481 cgccgttacc gatgattccg tcttcttctc cgccgattga accaacttca cattcaactg
      541 agataccttt tgcatgtgct ttttcaacaa cgtctttagc taatttcaag ttttcttcaa
      601 atggtagatg tgaaccatcg aacattactg aagtatagcc taattcgata cattccaaag
      661 cagcttcgta atcaccatgg tctaagtgga tagcaactgg aacagtgatg ttcattgctt
      721 caactaaatc agtgattaaa tctttacaga ttttgtaacc acccatgtat ttagctgcac
      781 ccattgatgt ttggataagt actggtgctt gttttgcttc agccgcttca aggattgctt
      841 gagtccattc caagttgttt gtgttgaatc cgccgacagc gtaaccgcct ttacgagcag
      901 cttttaaaaa ttcagctcct gatactactg gcataataaa attcctccta agttttgtaa
      961 aactaaattt gttaagacaa ttcttaacca acgaccctat tttaccaaac atttcggtag
     1021 ttttctacta aaatcaatga aaatgaaaat tttcaagaag ttttttgcaa aaaataaaat
     1081 atccaatagc gctttaatcg aaaaaagtct tttgctttct atcttttttc ctacaaataa
     1141 taacaaatca gaaaaagaga aaagtgacaa gaaaattgct tatccccttc tcttgctact
     1201 tttctctttc tatcaagttc aaaaattatt ttgtttcgtg atctgctaat gatgctccga
     1261 caaatgcttt gattaatttt tgcgggcgat ttggacgaga aatcaattct gggtggaatt
     1321 ggcatcccac aaagaatttt ttctctggca gctctacgat ttctactaaa cggttatctg
     1381 gtgagacacc tgaaaagact agcccttgtt cttcaaacaa ttgacgatat ttgttattga
     1441 actcatagcg gtgacggtga cgttcttgca caacatcggc tccatcatat gcttcagccg
     1501 tcttcgttcc ttttttgatt ttacatgggt ataatccaag gcgtaaagtt ccacccaaat
     1561 tctcgatgtt ttcctgatca gccattaaat caataatatt gtttgctgta tctggattcg
     1621 tttcagctga tgacgcatct tctaatccaa cgacgttacg tgcaaattcg acacaagcca
     1681 tctgcatacc aagacaaata cctaaaaacg gaacgtcatt ttcgcgagca taacgaatcg
     1741 cttcgatttt cccttctact ccgcgatcac caaatccacc aggaaccaag attccatctg
     1801 cgtctttcaa acgttcttct acattttctc tcgttaattc ttgtgctttg atccaatcga
     1861 tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa tgcttcaact actgagatat
     1921 atgcatctgg taactcgaca tatttaccga caagcgcaat ccgtactttt ttcttcaagt
     1981 tcaagacacg ttcttctaat tctttccatt ctgtcatgtc tgcttctggt gtatctagtt
     2041 ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg taaaactaat gggatggagt
     2101 aaagtgtatc tacatctggc gactcgataa ctgcttctgg ttcgacatca caaaattgtg
     2161 ccaatttgtt tttcacgctt tgagaaacag gttgctctgt acgtaccacc aaaatattcg
     2221 gttggatacc taaaccacgt agctctttta cactgtgctg cgttggttta gttttcattt
     2281 ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat atacataaca ttatctgtac
     2341 cgacatctgc tttcatttga cgaagtgctt caaggaatgg caaagattct atatccccta
     2401 ctgttccccc tacttctgta atgatcacat ctgaatcagt cattttagct gctcgcatga
     2461 ttttttcttt gatctcattt gtaatatgag gaataacttg aacagttgct cccaagtatt
     2521 cgcccttacg ttctttacgc aaaacttcag aatagatttt tcctgtcgtc acgttggagt
     2581 atttgttcaa attgatgtca ataaaacgtt cgtagtggcc cagatccaaa tctgtttcag
     2641 caccatcatc tgttacgaat acttctccgt gctgatatgg gctcatcgtc cccggatcca
     2701 cgttgatgta tggatcaaat ttttggatag ttacttttaa gccgcggttt ttcaataagc
     2761 gtcctaaaga agctgcaaca atcccttttc cgattgatga aacgacaccg ccggtcacaa
     2821 aaatatattt tgtcataagt tcaaaaactc ctttttaatt tatgtgcagg aaaagtctca
     2881 gacaataaac aaagctccct attcttcaat aagaataggg agccgaaatt tctgtaaact
     2941 tctgaccctc taaaagggtg cccaaggtgt ataatacaag ctgactaagg gaaagtcaag
     3001 tactcaaatg aatactatcc gcttacaatt ttttcttttt cgatatcctt tgagaaatta
     3061 tttcttaagt cctttatgag tgccaaaaaa atttaaccca cataataagc ttgatataaa
     3121 catagctaac gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa
     3181 taatagataa aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact
     3241 actttttcct acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt
     3301 cacccttatt tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat
     3361 atcttttcta ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac
     3421 ctttttttca acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa
     3481 aaaataagga gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct
     3541 gccaactcct cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt
     3601 atttcttgtg attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa
     3661 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
     3721 atttttctta atactcgaaa ctaaacgtct ttttcaaaac ctcttggtaa acggtgttca
     3781 aaagcttgca tctgcggtaa taagttcagc caattcaaaa acaagaaaag ctgtttttgc
     3841 ttggctattc ttattacttg ttgcagggca gctttttcac aacctctcca ctaaacggtg
     3901 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
     3961 ccgttagttt ctctaatact caggtcaaaa cgctgatttc acagcctcta attcacggta
     4021 ttcctgtttt aataaagttc tagatattgt tctctttccc actcggaaac agtttgtctg
     4081 aatgccgccc attcgagacg ttttgcttca acaaaatttg agaagatatg ttgtcctaat
     4141 gcgtcaatca ttacattatc cttacgcaat tctttgatag cattatggat agttgaaggt
     4201 aagtcctgga tatgtgcagc ttctctttct tcctcatcca ttacataaat gttacggtca
     4261 acagctgctg gcggtgtgat ctgattacgg ataccgtcta gtcctgcttg caacaatact
     4321 gccattgcca agtatgggtt tgcagatgga tcaactgacc gcaattccaa acgtgtggat
     4381 aaaccgcgag attctggtac acgtactaat ggcgaacgat tacggccact ccatgcgaca
     4441 taaacaggcg cttcataacc tggaacgaga cgtttataag aattgactgt tggattgcaa
     4501 acggctgtgt aggcacgagc atgtttcagc aaaccaccta agaaatgata agctgtttca
     4561 cttagttcca attcaccgtc tttatcatag aaaacatttc cttcatcatt gaacaaagac
     4621 atattgcaat gcataccaga accattgatc ccaaataacg gtttcggcat gaaagtcgcg
     4681 tgaagtccat gcttgcgtgc aatcgttttg acaacgagct tgaaggtttg gatattatca
     4741 caagcatcta ccacatctgc atatttaaag tcaatttcat gctgtcctgg cgctacttca
     4801 tgatgtgaag cttccacttc aaagcctaga ctttctaatt ccaacacgat gtcacggcgg
     4861 caattttcac ctaaatctgt tggtgcaaaa tcaaaataac cacctttatc attcaaggtc
     4921 gttgtgatcc caccgtcttc gtctaattta aataagaaga attctggttc aggtcctaaa
     4981 ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg ctcgtttcaa attcccgcga
     5041 ggatcgccgg caaatggtgt cccatccgga ttatagatat cacaaattaa acgagcaact
     5101 tttccatgag cactttccca cgggaagatc atccaagtgg agagatctgg atacaaatac
     5161 atatcgcttt cttcaattcg aacaaatcct tcaatcgaag aaccgtcaaa catcatttta
     5221 ttttctaata ctttgtcgat ctggctcact ggaacttcta catttttgat cgtacctaag
     5281 atgtctgtga acatcaatcg taaaaatcgt acgttttctt cctcaatgat tcgtttgatt
     5341 tcttcacctg ttatctgttt ctttaccatg gtatccctct tcctttttta atttattcag
     5401 tcaaagaatt gtctttgcaa tccttgcgac tgataatgac ctgttttgct taacccacct
     5461 tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat gatctgtcaa cggcttcttg
     5521 ttttttgctt cttgtttttt cgcctgtgcc atttcgtata cacgtttgat cccagccata
     5581 ttcaatccct ctgaaagata gtctttaatc tcaagcaaca tatcaatatc attcagcgaa
     5641 tacatacgtc ggtttccttc gcttcgttcc ggatgaacta aatcttgttc ttcataatag
     5701 cgaatttgcc gagcagacaa atcagtcagc ttcataactg taccaattgg aaatacggac
     5761 atagatcggc gcaattcctt ttctcccatc agacgtcccc tttcgtttta taagttggct
     5821 aaagtgtacc aattaaaaaa aatattgtca actgcatatg ttaggttttc taacatgaga
     5881 tctctatttt ttataaaata acaaaaaaat aagaacacaa aaaaggtatc tagttgattt
     5941 tcgtaccaga taccttttat tttttattat actgtactat cctttattag tgtttttatt
//
LOCUS       DACIFS010000103         5999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.107, whole genome shotgun sequence.
ACCESSION   DACIFS010000103 DACIFS010000000
VERSION     DACIFS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.107"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..450)
                     /locus_tag="IYA83_002495"
     CDS             complement(<1..450)
                     /locus_tag="IYA83_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8587891.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IYA83_002496"
     CDS             674..1465
                     /locus_tag="IYA83_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ8587892.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IYA83_002497"
     CDS             1431..2384
                     /locus_tag="IYA83_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ8587893.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IYA83_002498"
     CDS             complement(2782..3756)
                     /locus_tag="IYA83_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587894.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IYA83_002499"
     CDS             3871..4305
                     /locus_tag="IYA83_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ8587895.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IYA83_002500"
     CDS             complement(4430..5416)
                     /locus_tag="IYA83_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ8587896.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5999
                     /locus_tag="IYA83_002501"
     CDS             5844..>5999
                     /locus_tag="IYA83_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ8587897.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMA"
BASE COUNT         1888 a         1131 c         1034 g         1946 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggca
//
LOCUS       DACIFS010000104         5727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.108, whole genome shotgun sequence.
ACCESSION   DACIFS010000104 DACIFS010000000
VERSION     DACIFS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.108"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            196..921
                     /locus_tag="IYA83_002502"
     CDS             196..921
                     /locus_tag="IYA83_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587898.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IYA83_002503"
     CDS             911..1420
                     /locus_tag="IYA83_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587899.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IYA83_002504"
     CDS             complement(1490..2938)
                     /locus_tag="IYA83_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ8587900.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IYA83_002505"
     CDS             complement(2938..3654)
                     /locus_tag="IYA83_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ8587901.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IYA83_002506"
     CDS             complement(3635..3985)
                     /locus_tag="IYA83_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ8587902.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IYA83_002507"
     CDS             4129..4902
                     /locus_tag="IYA83_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ8587903.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IYA83_002508"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IYA83_002508"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACIFS010000105         4807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.110, whole genome shotgun sequence.
ACCESSION   DACIFS010000105 DACIFS010000000
VERSION     DACIFS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.110"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IYA83_002509"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IYA83_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ8587904.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..3768)
                     /locus_tag="IYA83_002510"
     CDS             complement(3250..3768)
                     /locus_tag="IYA83_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ8587905.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1578 a          786 c          731 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagggactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccactta
//
LOCUS       DACIFS010000106         4806 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.111, whole genome shotgun sequence.
ACCESSION   DACIFS010000106 DACIFS010000000
VERSION     DACIFS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.111"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYA83_002511"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="IYA83_002511"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ8587906.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="IYA83_002512"
     CDS             complement(1276..2112)
                     /locus_tag="IYA83_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587907.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="IYA83_002513"
     CDS             complement(2296..3513)
                     /locus_tag="IYA83_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ8587908.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3896..3979
                     /locus_tag="IYA83_002514"
     tRNA            3896..3979
                     /locus_tag="IYA83_002514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4806)
                     /locus_tag="IYA83_002515"
     CDS             complement(4219..>4806)
                     /locus_tag="IYA83_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587909.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1460 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaaa
//
LOCUS       DACIFS010000107         4744 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.112, whole genome shotgun sequence.
ACCESSION   DACIFS010000107 DACIFS010000000
VERSION     DACIFS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.112"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            25..696
                     /locus_tag="IYA83_002516"
     CDS             25..696
                     /locus_tag="IYA83_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ8587910.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IYA83_002517"
     CDS             755..1195
                     /locus_tag="IYA83_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587911.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IYA83_002518"
     CDS             1207..1875
                     /locus_tag="IYA83_002518"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ8587912.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IYA83_002519"
     CDS             1939..2562
                     /locus_tag="IYA83_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ8587913.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IYA83_002520"
     CDS             2618..3004
                     /locus_tag="IYA83_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ8587914.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IYA83_002521"
     CDS             3217..4608
                     /locus_tag="IYA83_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ8587915.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DACIFS010000108         4744 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.113, whole genome shotgun sequence.
ACCESSION   DACIFS010000108 DACIFS010000000
VERSION     DACIFS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.113"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..142)
                     /locus_tag="IYA83_002522"
     CDS             complement(<1..142)
                     /locus_tag="IYA83_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8587916.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IYA83_002523"
     CDS             complement(183..437)
                     /locus_tag="IYA83_002523"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ8587917.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IYA83_002524"
     CDS             complement(548..802)
                     /locus_tag="IYA83_002524"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8587918.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IYA83_002525"
     CDS             complement(995..1270)
                     /locus_tag="IYA83_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ8587919.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IYA83_002526"
     CDS             complement(1242..2195)
                     /locus_tag="IYA83_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ8587920.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IYA83_002527"
     CDS             2807..4300
                     /locus_tag="IYA83_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ8587921.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IYA83_002528"
     CDS             4435..4731
                     /locus_tag="IYA83_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ8587922.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1531 a          795 c          827 g         1591 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 ttta
//
LOCUS       DACIFS010000109         4657 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.114, whole genome shotgun sequence.
ACCESSION   DACIFS010000109 DACIFS010000000
VERSION     DACIFS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.114"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            188..418
                     /locus_tag="IYA83_002529"
     CDS             188..418
                     /locus_tag="IYA83_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ8587923.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            515..1816
                     /gene="murA"
                     /locus_tag="IYA83_002530"
     CDS             515..1816
                     /gene="murA"
                     /locus_tag="IYA83_002530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ8587924.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1829..2005
                     /locus_tag="IYA83_002531"
     CDS             1829..2005
                     /locus_tag="IYA83_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ8587925.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2140..2436
                     /gene="yidD"
                     /locus_tag="IYA83_002532"
     CDS             2140..2436
                     /gene="yidD"
                     /locus_tag="IYA83_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ8587926.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2621..3088
                     /locus_tag="IYA83_002533"
     CDS             2621..3088
                     /locus_tag="IYA83_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8587927.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3173..4057
                     /locus_tag="IYA83_002534"
     CDS             3173..4057
                     /locus_tag="IYA83_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ8587928.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4407
                     /locus_tag="IYA83_002535"
     CDS             4174..4407
                     /locus_tag="IYA83_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ8587929.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4495..4575
                     /locus_tag="IYA83_002536"
     tRNA            4495..4575
                     /locus_tag="IYA83_002536"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4529..4531,aa:Tyr,seq:gta)
     gene            4582..4653
                     /locus_tag="IYA83_002537"
     tRNA            4582..4653
                     /locus_tag="IYA83_002537"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gln,seq:ttg)
BASE COUNT         1573 a          724 c         1022 g         1338 t
ORIGIN      
        1 tttggaataa gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag
       61 catttatttc ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct
      121 tttatacatt ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga
      181 cgtcattatg caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt
      241 tatctactta gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat
      301 gcataatacg caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc
      361 aagtaatttt ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat
      421 tttaaaaaaa ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag
      481 tgtatgctca ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg
      541 caatcaatta aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt
      601 agctgctagt ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc
      661 tgatgtcttc actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga
      721 acaaaagaat caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga
      781 gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg
      841 acatgcaaaa gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca
      901 tttaaaagga tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc
      961 aattgcggat gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac
     1021 tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc
     1081 tcgcgaacca gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta
     1141 tggagctggt actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca
     1201 ctcaatcgta caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca
     1261 aggcaatgtg ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt
     1321 gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa
     1381 acatatctta cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca
     1441 agctcaaatg acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt
     1501 gtttgaaaat cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat
     1561 cgacggaaat gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc
     1621 aacagacttg cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac
     1681 acgtgtgcgt aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca
     1741 gcaattaggt gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc
     1801 acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt
     1861 gataaagatt ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat
     1921 cggatatggt gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat
     1981 tcatattcga gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa
     2041 gtgagccatg aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg
     2101 atcccttttt ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat
     2161 ttagttagag gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac
     2221 ccaacctgtt ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg
     2281 gtaatgggga ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat
     2341 gtaccattga cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa
     2401 aaggttgtga aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc
     2461 ttatattttg caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa
     2521 aaaggttgtg aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc
     2581 aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa
     2641 gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt
     2701 atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact
     2761 gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta
     2821 tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact
     2881 cgcttccagt caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca
     2941 tctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca
     3001 ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat
     3061 tcacttacct aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat
     3121 gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat
     3181 attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat
     3241 taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt
     3301 tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc
     3361 tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca
     3421 tggatttaat attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa
     3481 tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga
     3541 tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat
     3601 caataaaggg gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa
     3661 gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg
     3721 tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc
     3781 aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat
     3841 tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg
     3901 gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga
     3961 tttcgaaccg cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc
     4021 agcaaactat ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt
     4081 gcataaaaga agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct
     4141 gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa
     4201 gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta
     4261 tttataggga aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt
     4321 ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg
     4381 attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg
     4441 caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga
     4501 gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga
     4561 atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc
     4621 cctcatgcgt tggttcgaat ccagctaccc cagtata
//
LOCUS       DACIFS010000110         4603 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.115, whole genome shotgun sequence.
ACCESSION   DACIFS010000110 DACIFS010000000
VERSION     DACIFS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.115"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            43..113
                     /locus_tag="IYA83_002538"
     tRNA            43..113
                     /locus_tag="IYA83_002538"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="IYA83_002539"
     tRNA            134..218
                     /locus_tag="IYA83_002539"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="IYA83_002540"
     tRNA            280..352
                     /locus_tag="IYA83_002540"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            362..433
                     /locus_tag="IYA83_002541"
     tRNA            362..433
                     /locus_tag="IYA83_002541"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            451..535
                     /locus_tag="IYA83_002542"
     tRNA            451..535
                     /locus_tag="IYA83_002542"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:caa)
     gene            790..1665
                     /locus_tag="IYA83_002543"
     CDS             790..1665
                     /locus_tag="IYA83_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8587930.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1769..2533
                     /locus_tag="IYA83_002544"
     CDS             1769..2533
                     /locus_tag="IYA83_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ8587931.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2550..3962
                     /locus_tag="IYA83_002545"
     CDS             2550..3962
                     /locus_tag="IYA83_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ8587932.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            3979..>4603
                     /locus_tag="IYA83_002546"
     CDS             3979..>4603
                     /locus_tag="IYA83_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ8587933.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1462 a          717 c          994 g         1430 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttattc
      361 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      421 ccagctaccc cagttattat ttttaaattt gccggcgtgg cggaattggc agacgcgctg
      481 gactcaaaat ccagtgtccg ttgaggacgt gccggttcga ccccggccgc cggtataatt
      541 atcaatatag caactttctt atagagagtt gctatatttt ttttgttttt atgtaattac
      601 ctccaaatca attcgataca agtattatca taaaacgagt aaaaaagata ttgataacgc
      661 ttttattatg tgttaaacta tttttgtaaa ttatgtaggg tgctactatt caattaggca
      721 cgacctaaga aagggttctt tcttaggtcg ttttttgtct ttaaaaattg tttggagaca
      781 aaaatatgta tgaaggagtc tgcaagttgt tttgttttat catatagagg aggtgaatta
      841 acggtgtatg tgatgaaaaa gctgaatcat aatgctgtat tgatcgatga cgatggaata
      901 gaaaaaattg cgatggggaa agggattggc ttttctgcta aagtgagtaa gccatttgat
      961 gttgaggatg ctgacaaatt atttgtatta gattctaaag aaaagatcaa acgattcagt
     1021 gagatagtct cgcagattcc aatcgaattt atggattttg cggaagaagt gattgaactg
     1081 ataacagcta ggatctcctc tccattagat tcaaatatct atattacatt gacagatcat
     1141 atttattttg ctattcaacg aaaaagagaa gatagagatg tcacagcaat tatgttgcca
     1201 gaaatgagat tcctttatcc aaatgaatat gatatagcag tagaagtggt cgagcgaatc
     1261 aatcatcgat atcaaaccga gttagggtca aatgaagtag gattcatcac tatgcatatc
     1321 attaatgctg agctaggaga gcctaatagt ttaaagagct taaaaatagt agaaatgacg
     1381 accacgattt tgtcccatct agaaaaatcc tattcaaacg tttatgataa agaatcactg
     1441 acttatcatc gcttaatggt tcatatcaaa ttcctggtta aacgcttgat ttatcaggaa
     1501 gaaacgccag tagaaaatat tggttttttt actgaaacat ttaaacatag caaagcgtat
     1561 tgtacagcac taaagattaa agatatcatc gaacagcaat ttcaacttgc cattcaagaa
     1621 aatgaaatca tctatttggc aattcatcta gcaagaatct attaaaataa gggcgtaact
     1681 atctataggc gcaaccaaat gacacatagt aaatggacta tgattatttg gttgcgccta
     1741 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
     1801 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
     1861 atcaactaca ttgatggcaa atatgccggg tttcgagcat gctgtgaaac taagaaagga
     1921 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cactcctcaa
     1981 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
     2041 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
     2101 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
     2161 aaatcaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
     2221 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
     2281 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
     2341 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
     2401 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt acccaccaag tcgaattttt
     2461 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
     2521 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
     2581 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
     2641 cagctgacga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
     2701 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     2761 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatta
     2821 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     2881 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     2941 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctgtt tttttgggct
     3001 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     3061 gagcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     3121 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     3181 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     3241 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     3301 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     3361 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     3421 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     3481 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     3541 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     3601 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     3661 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     3721 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     3781 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     3841 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     3901 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     3961 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     4021 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     4081 atacgtttat atgatattga tgaagaaaga aatatggata tgtacttgat tgttaaattt
     4141 atgctgaaaa gaaaaggttt ttcaaatatt cgcattcgtg caacggatga tccaaagctt
     4201 gcttttacag gatgcgattt tgttttctca caaattcgtg ttggaggatt ggagatgcga
     4261 gaaaaggacg aaaagattcc acttaaacat ggattagtag gacaagaaac gtgtggactc
     4321 ggaggatttg cttatgggat gcgctcaatc aaaggtttat tggaaattgt cgatcatata
     4381 caagactatg cccccaaagc atggattttg aattatacaa atccagagtc gatcgtttct
     4441 gaagctgttc gtcgtaaata tccgaatgta aaaatgatta atgcctgtga tatgactatt
     4501 tcaattgaag aaacgattgc agtgaattac ggttatgatc gtaagaattg gatcgttacg
     4561 tattatggtt taaaccactt tggctggtat acatcgattt atg
//
LOCUS       DACIFS010000111         4305 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.116, whole genome shotgun sequence.
ACCESSION   DACIFS010000111 DACIFS010000000
VERSION     DACIFS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.116"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            148..1137
                     /locus_tag="IYA83_002547"
     CDS             148..1137
                     /locus_tag="IYA83_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ8587934.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="IYA83_002548"
     CDS             1134..1553
                     /locus_tag="IYA83_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587935.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="IYA83_002549"
     CDS             1562..1870
                     /locus_tag="IYA83_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587936.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="IYA83_002550"
     CDS             1874..2236
                     /locus_tag="IYA83_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587937.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="IYA83_002551"
     CDS             2458..3864
                     /locus_tag="IYA83_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ8587938.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DACIFS010000112         4234 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.118, whole genome shotgun sequence.
ACCESSION   DACIFS010000112 DACIFS010000000
VERSION     DACIFS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.118"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(37..1257)
                     /locus_tag="IYA83_002552"
     CDS             complement(37..1257)
                     /locus_tag="IYA83_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ8587939.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IYA83_002553"
     CDS             complement(1277..1882)
                     /locus_tag="IYA83_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ8587940.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IYA83_002554"
     CDS             complement(1931..2863)
                     /locus_tag="IYA83_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ8587941.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IYA83_002555"
     CDS             complement(3284..4144)
                     /locus_tag="IYA83_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587942.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1203 a          853 c          695 g         1483 t
ORIGIN      
        1 tttattcttt atttaaagct ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa gata
//
LOCUS       DACIFS010000113         4028 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.119, whole genome shotgun sequence.
ACCESSION   DACIFS010000113 DACIFS010000000
VERSION     DACIFS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.119"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            958..1926
                     /locus_tag="IYA83_002556"
     CDS             958..1926
                     /locus_tag="IYA83_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ8587943.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1943..2467
                     /locus_tag="IYA83_002557"
     CDS             1943..2467
                     /locus_tag="IYA83_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ8587944.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2843..3124
                     /locus_tag="IYA83_002558"
     CDS             2843..3124
                     /locus_tag="IYA83_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587945.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3126..3542
                     /locus_tag="IYA83_002559"
     CDS             3126..3542
                     /locus_tag="IYA83_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587946.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1535 a          712 c          671 g         1110 t
ORIGIN      
        1 ctttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
       61 cgtaggacgt actgaaaggg tgggatttat gagcgaacta atgcaaaatt tggctagcaa
      121 aaaaagttaa aaaatatatg ctaataatcg agcgaaatga acactttttc aagtgagcga
      181 atccgccatg acaaagctga agcagtttat ttcagctttg tcatggcgtg tgcctagact
      241 tttaaaaaag tcggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac
      301 gtttgaaacg tctaagtata taaatatata agagtaggtt taagagtgct taaatcgaat
      361 tttagagggc attcagacaa ctttagggtt gttagggctt gccctgaccg cctgtaaggc
      421 gctttaagaa caagataagg gatttatact aagactgtct taaaacgtct caaacgaagg
      481 ctcatagctt ctcaaagagt tccttatgaa catttttagc aaaggagccg gaagcgactg
      541 cgcagtataa tgtgagagca gagcgatcac acctttttaa gcgactgtag ggagtaagat
      601 tcccagttga gaaaaatgcg cgaatctgtt tggagaaaaa agaagtggtt ttttaggcgg
      661 gaaattttgt ccccctcttt tataatctct ttttaaacct ctttttaaac ctctttaccg
      721 tcggctatat ccttactctc ccaagggaat gacgctttta tctacatcgt tttgtctgtt
      781 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
      841 tctgtttgta cattgaatta gtacatcgac gatgttaata taagatgtag ataaacaggc
      901 attttgtatt tcataacgtg tatctcttgt gcgagtaaga aaggagctat tttaaatatg
      961 tctaatgaaa tcgtaaagta tcatcatgaa ttaaatacaa ttccgttgcg aaaatttaca
     1021 ccagtcgaaa tgaatttgtt cttttctatc gtatcaagaa tgcgtgatgt aggtgataaa
     1081 aaagtacaat ttacattcga gcaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgttttgttt tatttaatca atttaaaatc
     1261 gacggaaaat ctgatattcc ttttgctgaa atacaagttc atgaaaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggtgcgctat tctctccaac aattcaatga attagaaagc
     1381 agttattcaa aaacaatgtt tcgattacta aaacaataca gaaccaaagg tttcgcttat
     1441 ttttcaaaag aagactttca tgaattactt gatattccta aaagttacaa ccaaggtacg
     1501 attgatcaac gtgtgttcaa tccaatacga caagaactaa ccgcaatctt taaaggttta
     1561 acaatcaaaa agaaatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgccccaac aaaagaagaa
     1681 caaaacaaag cattagaact agctaaccaa caagttcaat ctgaaaaagc acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaagcga aaattggaac ttttacaaca gttagaaaag
     1801 ttagaaagag aacaaaatac ccaaaataaa gaaaataatg cacaaaaatc agagataaat
     1861 tccgatattc cttcagaact aaaaaaagac ttattagaca atcttcaaaa cttattcaaa
     1921 gattgagggg tttttagatt ttatgacaga aaaaacaatc aaagaacttg ctgaagaatt
     1981 aggagttagt aaaacagcaa ttaacaaaaa agtttctgac gaaaatcgaa aactttggtt
     2041 ttcgaaaatc ggaaacaagt ttgtgattaa tgaaaccgga caaaaggcta taaaatcaat
     2101 gttttttaac gaaaacgaaa actcaaatca acaactagtt ggcgaaaaaa cgaaaaccga
     2161 aaaccacaaa aacgccaacc aagttcccga attagaagtc ctttctctcc tagaacaaca
     2221 gttaaatcaa aaagatatgc aactacaaga aaaagacgag caaataaaac aaatgcaaaa
     2281 attactcgac caccaacaac aactcaccct gcaagccaac caacaaatcg agaaactgca
     2341 ggaacaacta cagttgactt atacggaaga atcttcggaa aataaatcaa cgactctttc
     2401 tgaagaaaca gaatcgatag aaaaacaatc aaaggcggag aaaaaatggt ggcaattctg
     2461 gcaataaaac cagcaattta aattaataaa aattgctggt tttattcttc tttttgcttt
     2521 tgcgagcaga gcaacatgga cccatgttgc gatattttgg tggcgcccca atccccgttt
     2581 tttcgttgaa tcttactcgg actgcactcg ttttacagct gatttttctg aattttgaaa
     2641 gagcataagg aaacgtggta tcactttctt acgaaaggtg ctagataggc tgtgcctatc
     2701 acgtttctca gaaaaaaatt tgctatcact tttttgccat tgtggctagg ctatgtatag
     2761 tgttttggcg aaaaatagct atacaaatac tatatttgta tggtatacat aaattatacg
     2821 ctgatacgaa aggaaaattt ccatggacat tttagttcga aatatcaatc ccaaaattat
     2881 tgcaagaata gataaacgtt gtcaggaact cacaaccaaa acgggcaaaa aatggagtcg
     2941 aaataaatat ctaaaagttc taatcgaaag tgattttgac cgtcaactta tgaattacaa
     3001 aaaagatcaa ttcgatttat tactcgaaaa atttattgcc attcaaacag aaaatacgaa
     3061 catactgaat gactatgttc acacaaacaa ccaactcatt gaaacactta tcggaggaat
     3121 ataacatgag aaaaacttct gttcaaaaac acgtaaccct ttatcccagt cacgttgacg
     3181 ttttagattc catcatcgaa aaaaatccag acgtcaacaa ttattcagaa gccattcgtt
     3241 ttttatgtat gagttatgaa cctgatacca atacaaccac aaccagtaac caagcaaaac
     3301 tcaatgccat gggaaaagaa atttctatca tgacagaaat catggcagaa tttgctgatt
     3361 tatatctaaa agatactggt gttcttatcg gtacaaattc taaagtgtat caagaagtga
     3421 aagaaaaaat tgaaaatcaa ctcaatcctt cccgacgaaa tcaacccaaa aatcgtcaaa
     3481 aagaaaccaa tcaaaccttt acttctggaa attttgaccc aacaaacttt ctaaaaagat
     3541 aaaaaaagaa accgatccct aaaaaaattc ctcgatttct ttttagggat cagtcttcca
     3601 gcaagatttg caagttggta ccaacttgcg aaaaaaaaga aaaatccgct tatgcaactt
     3661 cttctttttg tattttgggg cgctacccca atccccaagt atcggttatt taatagcgcg
     3721 gaaatccaaa gaataggacg taccctgaaa caaaaaaaga ctgctctcac gagcagccca
     3781 aaatgagatt atcctattca tcaacataaa aatattacaa agtgaagaac tagagaaaga
     3841 caaaaaaagc atatcaaatc ctctcaaaaa taacaagtct ttaaaaaatg acagataaaa
     3901 atgcacaaag aactaaaaaa gagaaatcgc gtaaaatcaa gcattagaag ctagtaacta
     3961 gctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag gggctacttg
     4021 gtctagtc
//
LOCUS       DACIFS010000114         3979 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.120, whole genome shotgun sequence.
ACCESSION   DACIFS010000114 DACIFS010000000
VERSION     DACIFS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.120"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..1257)
                     /locus_tag="IYA83_002560"
     CDS             complement(<1..1257)
                     /locus_tag="IYA83_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ8587947.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEA"
     gene            complement(1308..2129)
                     /locus_tag="IYA83_002561"
     CDS             complement(1308..2129)
                     /locus_tag="IYA83_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ8587948.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(2126..2935)
                     /locus_tag="IYA83_002562"
     CDS             complement(2126..2935)
                     /locus_tag="IYA83_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ8587949.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(2956..3450)
                     /locus_tag="IYA83_002563"
     CDS             complement(2956..3450)
                     /locus_tag="IYA83_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ8587950.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(3462..3950)
                     /locus_tag="IYA83_002564"
     CDS             complement(3462..3950)
                     /locus_tag="IYA83_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ8587951.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         1218 a          933 c          656 g         1172 t
ORIGIN      
        1 ggcttcaaaa atttctgcaa gtccactaca ggccttccaa agacaaagat tgctgccggt
       61 atgatacttt ccaagtgcaa aggcgtctga taaccaaaca gatggaaaaa gcattggttc
      121 ccctgaccga cgttgagcta atatgtcctc aataattctt gaagcattca gtagaacctg
      181 cggataccgc tcaaagaatt ttgtatcccc agtatgttca aaataaagac tagccagtaa
      241 cgcactggat aacgaattac tcagagaatg attgatacct cctgtaaatt ttgaaccagg
      301 aaacttaacg ccatattgat caaaccatag aaccgttttt tgacataact ccggttcaaa
      361 catggtgaat ggcaatgatg cgtaatacat atccttattc caaattcgat tagtagcagg
      421 atagctgccc caattgctgc cgacgacctg tccctgtcga ttcataccga aactagaaaa
      481 gctttgataa agtgcacgac gatagagatg gacaactcta tcgtttggca ttgacagctg
      541 cccaaatagc tgagcaaaat agttcaatgt ttccttcagc catttttcga tatcagattc
      601 ctgaaaagtc attacttctt caaaagcatt tgggtccaca aaagcaatgg cgaagcttgc
      661 cacttcctgt tgtgcgatcg tgtccttatt tcctttttga gaaatcaaca cattttgctg
      721 atccgaatac ttctcttggt acaaaggcac atctttcact gaaacttgaa ggctttcgtg
      781 tgtttcattc acaatgtaaa cctgttgaac aagcatactt aaacgcttat tagccagtat
      841 tggagcaaag gatactacaa tgaccttaaa cgtcgaataa tgatgaatat atctaggcaa
      901 acagtcttga atcacatcga cttcaatacg atgatccgat tctgacaaat ggtagacaat
      961 cccgtttact tcaaccttta aggataacga attggtttga gaaagctgtt tattgaccca
     1021 aacacctggt ttttcctcag ttagtaaatt ttctcgataa aaggtcaacg tttgaatcgt
     1081 cccgcaaaca tcaaggtttc ctaatgtctt ttgattactt acatcgaagg tcaatccctt
     1141 tacatcagga atattccgat agtagctgta ttcgtttgaa gtagtcacat ttccgtcgtt
     1201 taactcagca ttaaataatg ggtgtttggg tgaataaatt acttcattaa tagtcatctt
     1261 ttccctcctt gctaaaaaaa ctgcagccta gtagctgcag cttcctacta tgcgattcca
     1321 agtaatgaca ataaaattcc caagacgacc attccaaaaa taattttgat gacgccgact
     1381 tttttatcta gtaatttgaa acagcccaat gttaccaata acggtacaat tcctttaaaa
     1441 atactatcga ggatttcttg gacattcatt agttcttcgc ccttcattga aaagctcaaa
     1501 acggtattga atttgaccat actgatcgtc attgcgccta ccatgatcag acccaagata
     1561 ctagcagctt ttgtcagaat ttcaattaac ccgttcgcat acagcttttc aatatattta
     1621 gagcctaatg aatagcctaa aacgccgcca tagtatcgca tcaaataatt aggtatattg
     1681 aacataacta aaaagataat tggtgccaaa atatttccgg cattggctaa gttgatcgct
     1741 agtcccgcgg taacgacacg ccaaactccc cagaacaatg aatcgccaat tccagccaat
     1801 ggtcccatca aactcgtttt gatcgcgttg atactttcca cgtcaaaacc agctttttca
     1861 gaattttcct tctccatcga tgccgcaatc cccatgacaa aggacgcgca aggaatggtc
     1921 gtcgaaaaat actgtgtatg acgttccagc gccgcagctc gatcttcttc acttttgtag
     1981 aaatgattaa tgaagggcat catagaatag gcgaaaccac cgccgcccat cgtcaccgga
     2041 ttcaccgaag catacatagt catcgagcgc caaaacatct tccgaaccaa ttttttctca
     2101 tctttagaaa ttccatcagc taatttcatt gaaagaaatc ctcctcttct tggtcaagcg
     2161 ttgcatttac agcagcatcg ctttgaactg catgaacaga tatatttttt agttgtagat
     2221 ctctttgggc tataacaatt gcaataatcg ttccaactgc ggcaattgca atcaatggta
     2281 actcgagata cgccataaca acggccccaa agaaaaacca cacagcaata tttttactcc
     2341 aaagcatccg taacagcata gccatcccaa ctgccggcag cagatttcca gcgactgtta
     2401 atccgttcat gatgacctct ggaatattat ccaagacgct tttgacaaca tctgctccaa
     2461 ataaaacggc aaagaatgga ataagagcat aaagcgcata gtggacaaac cacataacga
     2521 agtgcagatt gatgattcct ttttgatcac cagattttgc aaatttgaaa aatttagcag
     2581 ccatcggtcc acatacaaaa gaaaaaatca tattcattaa ctgtaatcct aatactgcta
     2641 gtggaaagac aatgggaata atcgcatcca cgtttcctcc cattttgatt gcgaacgctg
     2701 tagccagccc agcagccaaa gaaggctccg ccgtactaga gattccgata ttgacgacac
     2761 ccataaagat ggcttctagt gacgccccga caatcaaccc cgtctgcaaa tcccccatca
     2821 acgctcctgc taacggagca acaacaatcg gacgactcag catggacatc ccgaacattt
     2881 catgtccagc aactccaata aaaacggcca aagcgactac aaatgcatca tacataattt
     2941 ttctccttcc tttttctaaa aatcactcaa aatagagata gctgaacgtt tttccatcgc
     3001 cggtgtaggc tgaaaaacgg tatctggata aagttcaact aattctttta agtcttgaat
     3061 ttctgacgtc gttaacatca cttcctttga taaggttttc ttatcttccg atggcggtgt
     3121 cattttccca gcattcccca cgttcattaa ttcgatttcc gttgggaaag ctttcgcaac
     3181 ttttaccgca tctttgacat tagggactaa aaccatcaag cgcatgtcct tcgcccgcgg
     3241 atcttttaac aaatgaatcg cgtcatcaac tgttttaata atgactttga cattcgatgg
     3301 tgcagccatc ttcaatgtca tcttttgaat atcgttggta gccgcctcat cgcttgctac
     3361 tactaacccg gttaaattta gactctttgt ccaagtcatc gctacttgtc catgaattaa
     3421 tcgttcatca attcgcactg ctttaatcat ttttcttcct cctaaaaaaa atcttcctct
     3481 tcatttacta catgtacctc atcaagaatg gtcatcttta attcctgttg gcatttattt
     3541 accagttcct gaatatcctc ctttgttaat tccttggttg ccaatactat ttcgagcaga
     3601 attggcagat taaagccaga caaaatagtt acagcaatct ctttctccaa aaaggttttg
     3661 gtaatcttct gattgacact tccgccaaat aaatcggtaa aaacaaaaat ctgatcctgc
     3721 tcgttcacct ggtttacaaa tacattcaac tcctgttcaa aatcacactc atccacataa
     3781 gcattaatca ccgtaatctc ttgaaccttc cccgtcagta attgaatact gctaagaaag
     3841 cctgaagcta agtgaccgtg agtcgctatt agaattttct tcatatggtt aattcctttc
     3901 tttctgttca cttttatata agcaattcct gtgccaactt ttaaaaacat tggtttaatc
     3961 acgttaattc acattaaaa
//
LOCUS       DACIFS010000115         3682 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.122, whole genome shotgun sequence.
ACCESSION   DACIFS010000115 DACIFS010000000
VERSION     DACIFS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.122"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..278
                     /locus_tag="IYA83_002565"
     CDS             <1..278
                     /locus_tag="IYA83_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ8587952.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            421..642
                     /locus_tag="IYA83_002566"
     CDS             421..642
                     /locus_tag="IYA83_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8587953.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            658..1500
                     /locus_tag="IYA83_002567"
     CDS             658..1500
                     /locus_tag="IYA83_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587954.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1563..3365
                     /locus_tag="IYA83_002568"
     CDS             1563..3365
                     /locus_tag="IYA83_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ8587955.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3424..>3682)
                     /locus_tag="IYA83_002569"
     CDS             complement(3424..>3682)
                     /locus_tag="IYA83_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ8587956.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1313 a          521 c          750 g         1098 t
ORIGIN      
        1 aagaactcat caagctggct aataagggtc aagattatga acatctagca gatgagattg
       61 atgagctgag agacaagcga cagctccttt tagtagaaga tgcctccctc agtggcgaga
      121 acgagcgaat caatgagctg attgaattta tccgcaagaa caaattccgt accttagagt
      181 acgatgataa gcttgtaagg aagataatcc agagcgttac agtctatgaa gaacacttcg
      241 tcatatcctt caaatctggc atcgaaatgg aaatatgaaa accagaagta aatagcctat
      301 gattctacag tagagtcgtg ggttttcttt tgcttgctga ttataataat attttaataa
      361 ctattgttat tataaaccaa atgatttata atattcttag cgtaaacttg gaggtgctgt
      421 atgactacgg cagaaatgat taaagaactg tgtgagcaaa tgaatataag tgtttccgaa
      481 cttgctagac gtattggaca gactccacag aatttcaata agaaattaca acgagaaacg
      541 gtaaccttag atgagttgaa agcgattgct gatgtgctag gtgtcaagtt tgtgcaggct
      601 tttatattgc ctgatggcaa tgaaataaag ataggtaatt aggaggtgta atttgtattg
      661 aaaatcataa caataaatga tgttgagtat gctgtatttg ctgctaatga aggtacttcc
      721 aaaccacaac ctcatatcat agaaacgaaa tctggtacta ttccagaagg aaaacaactg
      781 agtttattaa aagaatatct taagcagaat gatatttccc caataaaagg agcaacaaca
      841 tattggtgca ttgacaaggt tcttaagctt gattcaagta aagaaaaaac tataagtgaa
      901 acaatccata aacaaaaata cttatcttta actgaagaaa atattgagaa acagcataag
      961 tttgtaggtg caagctctaa ttatgggaaa gaaggtctaa taatacatga tgttttgaat
     1021 gcatttcctc ttcataatga tttaaatact attgcgatga agattgcggt aattgatgta
     1081 acaaattcaa cacatctttc tcaatataag agcaggttgt ctctatatga tttggctaaa
     1141 gtaattctgg agattccaaa ttttgatgat cggttggcaa aaggagatcc tcagttgata
     1201 aatattatcg caaggaacat tggtgcagtt aatatgttct catttgcatc caagtactgt
     1261 acatatcata atgtggaagt gtgtgggaga gatgattact ccatctttga tggtatcgtt
     1321 aaaaatactc ttccacacta tatccaagga ctaactacga ataagattga tacgtggcga
     1381 agaagttttg attacgaggc atttaacgaa tgtgttggaa aactacttga tgaaaataat
     1441 atacatattc cttttagaag aagaaagtta gatcacttct tgtggtatgc gaataggtag
     1501 aatataagaa aaaggcatcg taaaagtgat tctggataaa attaatgggg gaggaaataa
     1561 tagtgggatt atttgatttt ctaaagaaaa aacaaggaaa ggttagtaaa cacgttgatt
     1621 atcaactggc ttctagtcta tttcgtactg gtaaagtttt tgctgaaaaa ttggaagtgg
     1681 acatatcaaa tgtagatgag tttcgtaaaa cttttatcgc ttttgatgtg gaaacaactg
     1741 gcctaaattc ttcttcagat agaattgtag agattggtgc ggtgctattt aaagaaggag
     1801 ttccaattca atcattcgga actcttgtca atccaaaagt tagaattgca gcctcagctt
     1861 cggcaataaa taatataact aatgaaatgc taaactctgc tccaaatgag caggaagttt
     1921 acccccagtt ggtagaattt ttaggagatg cactagatgg aaaaatcact atgtgtgcac
     1981 acaatgcaag tttcgatttt ggatttttaa gtaacacatt ttctagactt gggtatgatg
     2041 ctaaaattaa gtatgttgat actctgagtc tctctagaaa acatataaaa ggcattgtaa
     2101 atcataagca agagactgta gcggaatatt tcggattaat aaatgacact gcccatagag
     2161 cggaatcaga tgcagatata tgcggaaaga ttttttgggg tgttttagat attattgagg
     2221 agtcgttgga agaacaaaaa aagcaaatag aaaagatgac tccagacata aaagaacttg
     2281 aagcttgtgc ttttattcaa gatattattg agaaaaaagg atgcgacacc agttggatca
     2341 ggtatcgaaa aaacagtaat aattatgtcg atgtaacttg tctatataca ttcttaaagt
     2401 ttaagtttgc taagaaggga aattacatca ttattagaaa agatgctaca aatggaattg
     2461 atttacctat agaaccttgt actgtaagtg aaggcggtac aacaaatgtg cgagtctatt
     2521 ttcgtagccc ctttgactta acgcctcttt caaattatat tttcagtgct tatagcgatt
     2581 gttataattc tatgaatgaa tatatgagtg ttagtaatta cactaaaaaa gaagcagagc
     2641 aaagccttag tatgctgaaa tctatttcaa aatcagcgat gaaagagtta ttattcgatg
     2701 caaaaaatcg tggatatgat gagactgaag ttaacctaaa tattgaaaaa gtgatatcta
     2761 gagaagatgt aattattaac gggttgcata atcgagtttc attaaacaag atacgaaata
     2821 tagggaattg ggagaaagga tttgatgcag gttatccata ttgggaacgt ggagaaactg
     2881 ctaggaaaaa tggcaaagta gatgaagcta ttgcactttt tgataaagct cgatataacg
     2941 gctatgatgc tcctgcgcta tatgattctt atgctaaagc ttatcgaaaa attaaggatt
     3001 acgataatga aattctcatt cttgatgaag gaattatgcg taaaacacgg cacgatgttg
     3061 gtactttaga ggctcgtagg gataaggcaa ttaagctatt atttgctaaa caagaggcag
     3121 agcggatagc taaggaaaaa tctgattttc taaaagcgaa caaaaaagag gatgcgatta
     3181 gtgagcctag caagaagcgt ggaagacaga ttattcaaat gactgatgat ggaacaataa
     3241 taaaagaatt cgatactata gcttctgctg taaaagaagt tggcgttaac tcaaaaagta
     3301 ttagggatgc tgcaaatgga gttcaaaaac atgctggagg atactactgg aaatacaaag
     3361 attagcagat aatctaagat aacctaggat cttcttaata gatacccgtg gtgctagtta
     3421 attttaatat cctaaagaat atctcaaagt tccttcaaaa cgtcgttgtg ctcgacttaa
     3481 tagaacactg taagccagta atatgctttc aaatctactc tctagccctt taacagaacg
     3541 tgagttgaaa ttttgacaac ctaatttttc taaggaagaa aaaacagtcc caactgtttt
     3601 tctttgtttt ccaagtaagc tgtggtctag tttttctgat ttatccatgt ttttacgtgg
     3661 cggaatggat atagtaatgc cc
//
LOCUS       DACIFS010000116         3524 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.124, whole genome shotgun sequence.
ACCESSION   DACIFS010000116 DACIFS010000000
VERSION     DACIFS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.124"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            919..1842
                     /locus_tag="IYA83_002570"
     CDS             919..1842
                     /locus_tag="IYA83_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ8587957.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1858..2397
                     /locus_tag="IYA83_002571"
     CDS             1858..2397
                     /locus_tag="IYA83_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ8587958.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1335 a          542 c          606 g         1041 t
ORIGIN      
        1 cttttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
       61 ccgtaggacg tactgaaagg gtgggactta tgagcaaatt aatgcaaaat ttggctagca
      121 aaaaagctaa aaaatatatg ctaataatcg agcgaaataa acactttttc aagtgagcga
      181 atccgccatg acaaagctga agcagtttat ttcagctttg tcatggcgtg tgccgacttt
      241 tttaaaagtc taggcacacg agcaaatatc tttatcaagc tctaatttgc gttttaagac
      301 gtttgaaacg cctaagtata taaatatata agaataggtt taagagtgct taaatcgaat
      361 tttagagggc atttagacaa ttttagggtt gttagggctt gccctgaccg cctgtaaggc
      421 gttttaagaa caagataagg gatttatact aggactgtct taaaatgtct taaacgaacg
      481 ctcagagctt ctcaaagggt tccttatgaa cggcttttag caaaggagcc ggaggcgact
      541 gcgcagtata atgtgagagc agagcgatca caccttttta gcgaaccgta gggagcaagg
      601 tttggaattg gcagttttga taaattcgcg aacctgttta gagaaaaaag gagtgatttt
      661 ttaggcggga aaattagcct cccctctttt tatatctctt tttatatctc tttttatatc
      721 tcttttatgt cggctgtgtc cttactctcc caagcaattc agagtttttt caagtaggtt
      781 ttctacgctt ttcaagtagg ttttctacgc ttttcaagta ggttttctac gcttttcaag
      841 taggttttcc gacattattg agtattttta taaagtatgt tacttttatt tcatacttta
      901 taaaagtagg agaagcttat ggcaaatgaa attgtaaaat atgaaaatag attaaatcat
      961 attcctctga gaaaatttaa tagtcgtgaa atgaacttat ttttttcaat tgcttcacgt
     1021 gtacgtgaaa aaggtgtaga tgagattatt ttttcatttc acgatttgaa aaaactaagc
     1081 ggttatacag aacgtgggga acaatttata aaagatctta gtagcacgta tgacaaatta
     1141 ttaagtatta atgcttggaa tgatgatgga aatactttaa ctaaatttgt tgctttcaca
     1201 aaatatagca ttatacgaga taaacaactt gttataattt cagttaatcc agaatttaaa
     1261 ggacttttta atcaattaaa gaactggaca agatttaatc tagagcaatt tactaatttg
     1321 gatagcacat attcaaaaac tatgtttcga ttattaaaac aatatcgaac tcaaggctgg
     1381 actgaatttt ccaaagaaaa attttttgat ttactagata ttcctaaaag ttattggaat
     1441 agtccgtcaa atattgacaa atttatctta aggccaatta aagaagaact cactccctta
     1501 tttaaaggat taactattcg taaaaaatat ggtaaaggtc gaggtaaacc tgttatcggt
     1561 tatcgtttta cttggaaagc tgaaattaac catgctgacg atttttccaa agggaaacaa
     1621 gaagatttac gtataaaatt atttaatatt gatcacaatg gagaattaac acgagaagaa
     1681 aaatggcgcg ctaaagatcg aatactaaat cttcccttag gcactcatga agctgatttt
     1741 aaacaacaac aacaaactga agaaaaacag gcagaaaaac gagaactttc aaacaaactc
     1801 aaacaagatt tactaggaaa tttacaaaac ttattcgatt aagcttaagg agtatttatg
     1861 gcagataaaa caatcaaaga acttgcggaa gaattaggag ttagtaaaac agcaattaac
     1921 aaaaaagttt ctgacaaaaa tcgaaaactt tggtttgcaa aaaacggaaa caagtttgtg
     1981 attaacgaaa ccggtcaaaa agctataaaa tcaatgtttt tagtcgataa cgcaaactta
     2041 aatcgaaaac tagttggcga aaaatcaaaa accaaaaacc ataaaaacac caaccaagtt
     2101 tccgaattaa aactcctttc tttcctagaa aaacagttaa atcgaaaaga tatacagcta
     2161 caagaaaaag ataatcaaat aaaacaaatg caaaaattac tcgaccagca acaacaactc
     2221 actctacaag cgaaccaaca aattgagaac ctacaagaac agctccattt aacttataca
     2281 gaagaaactc caacaaccga atcaactgcc ttttctgaaa aaaaagcaga caatacacaa
     2341 ccacttattg aaaaaaagtg gtggcaaatt tggaaatcta atagaaccaa tctctaaaat
     2401 tttctagaag ttagttcgga tagtagagga aaaagttgta ctacctgatg gggagatcac
     2461 tcaaattttt atgagaaaga aaaaatagtt tacaactgta aacaatcaat gtttatagtt
     2521 gtaaactatc aaactatatg gtgacttatt gcttaaaata ggttgaaatc tcactcaatt
     2581 tttggtggaa aaacaaaata aattctaatc gattttgttg aaaaagtcat aaaaaaacaa
     2641 atgtgatttt aacaaaattt caaaaaaatc gtttgaaatt tcgtttattt tttttgagaa
     2701 ttttgatcat cgtagggtca ttgtagaatc attatgagat catcgtggga tcatcgaata
     2761 aagtatggga aaggcttgcg gtgcttggta ttaagggcat tctccgcctc ccaggttctt
     2821 gaccattttc tctcaacgtg gtaaaaatcc cacttataag agaaaatggt caagaaccta
     2881 aacggtcggc gaagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca
     2941 aaaaagaaaa attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa
     3001 tttaaacatt tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta
     3061 aaaaaagtgg aggtataacc tatgttaaaa aaaactgtgt tggtactggg ttctatttct
     3121 ctaggaatat taggttttag ccccatgcta ttatccgtaa ttggaaaaaa caattccaac
     3181 ggagaactta aattaaattc aaattattac aacagtccta acgaagaatt gtttgtaaaa
     3241 gagacggata aataataaaa aagactgctc tttcgagcag ccagttaaaa aataatgagt
     3301 gtttattctt gaaaaagcaa cgcgcttatg aagcgctacc aaaaatttat catattttat
     3361 gaaaaataac accccagtaa aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc
     3421 aactgcagaa aatcaagcct taaaaagagc ttttgactta gaccaatcga aaatcacaaa
     3481 gtgagcccct gtatcccttg gtagagaggc tacttggcat agta
//
LOCUS       DACIFS010000117         3401 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.125, whole genome shotgun sequence.
ACCESSION   DACIFS010000117 DACIFS010000000
VERSION     DACIFS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.125"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(74..1072)
                     /locus_tag="IYA83_002572"
     CDS             complement(74..1072)
                     /locus_tag="IYA83_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ8587959.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="IYA83_002573"
     CDS             complement(1147..1443)
                     /locus_tag="IYA83_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ8587960.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="IYA83_002574"
     CDS             complement(1436..1729)
                     /locus_tag="IYA83_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587961.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1875..2426)
                     /locus_tag="IYA83_002575"
     CDS             complement(1875..2426)
                     /locus_tag="IYA83_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ8587962.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2600..2899)
                     /locus_tag="IYA83_002576"
     CDS             complement(2600..2899)
                     /locus_tag="IYA83_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ8587963.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2926..3192)
                     /locus_tag="IYA83_002577"
     CDS             complement(2926..3192)
                     /locus_tag="IYA83_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ8587964.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
BASE COUNT          993 a          668 c          461 g         1279 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacacacccc
     1741 ctgttatttt ttacataatg aaaatagcat attactgggt acattgataa ttcatataac
     1801 atgtatcatc acatgtattt ttaaagtcaa aaagcatgta aaactactta taagtaacat
     1861 gagaaaataa aatgttattt ttcttgaaat aataattgac tatctgccaa ttgccattca
     1921 ttacgaacat gaatacttag tgggtgggat aacttacctt ctgcatttat caatactttg
     1981 gtcatcttct tttgtttcgc actcaattga tcgtaaatag tctttgcttg ttcataagcc
     2041 acttgactcg tcgctgtaat cgaaccacaa gcaaaaataa tggtattgat tttcttttct
     2101 gtaaaaacag tcgttaatat ttccaaagat tgttcatcat ttcctcgaag aaatacccga
     2161 ggacttttcg cttttgtgta cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat
     2221 tgtcggatat tcttttcgat taataacata ctcaaatcat tcgtaaatgg ttctgtatct
     2281 gttggacgaa tagtaatgac tactgctaat ttatcttccg cttcttttcc tttgatcgcc
     2341 cattgtcgtt tgaaaacata gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt
     2401 gtggtcacta attctttttc aatcatctct ttttctccta tgctattttt tgttcaagca
     2461 cttcaattac cattgccgtt ataagtgtca atactagttg aaaatatttc atattctttc
     2521 tccttttttc ttcagaattt gatgaatcgc ggtaaggata tcttctaaac atttcgcaat
     2581 cattctcaaa taatcgatct tattcatccg ttcctccaat ctgactccaa accaatcgtc
     2641 cttctaccag taacccacga acaaattctc gctggctggt aatatctgta atgaaataaa
     2701 ctacatagtc ttttccatct tctggtgcgc aataaatata aagatgtgtc atcactaaac
     2761 gtggtagatg aacatcttca ggaagcatac ccatttcaat ttcctctttt tccatcgtaa
     2821 cggattcata gccccactct tcagaagcag tgaccagctg tgcacacttt tccagtactt
     2881 cgatagccgt ttctaacaag tcctttcgtt cctttctttt agatactagt attcttctgg
     2941 taataacatc gtttgatact cacccgaatc aatggcccat attttttgag acactgattt
     3001 caaacgaagc ttaggtgaaa tcagatactc ttttctttct tccggttctt cttgacgatg
     3061 aaccaacttt tgatttcctt tttccgtaga aaattcaaaa atttgaagat agtctaaagg
     3121 aaattgcttt tgaacgtttt gatcgattag attccaacat aaagcttgta attccaccgg
     3181 caaactgtcc attactccct ttgtgacata acgttatcct ttattttgaa acatttcttt
     3241 tccctccttt gccataaaaa tagcccaatc ttctattaaa gaagattggg cttcatggat
     3301 ataatatata tttgtaaata aagttaattc agttataatt atcaaaacta cacaattatt
     3361 aaagtttttc atatctcttt gtttagtaag tttttctaaa t
//
LOCUS       DACIFS010000118         3219 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.127, whole genome shotgun sequence.
ACCESSION   DACIFS010000118 DACIFS010000000
VERSION     DACIFS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.127"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            49..1437
                     /locus_tag="IYA83_002578"
     CDS             49..1437
                     /locus_tag="IYA83_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ8587965.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>3219
                     /locus_tag="IYA83_002579"
     CDS             1775..>3219
                     /locus_tag="IYA83_002579"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ8587966.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          947 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaagcct tgttagaaaa tggtgccatt gacaccgtca ttggcttacc
     2941 tgctaacatc ttctttaata cgtccattcc aacgaccgta attatcttga agaaagaccg
     3001 tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat tttgaaaaaa taaaaacgca
     3061 aaatacgcta cgagattacc atattgacgc tattttagag gcctataaaa cccgtacaat
     3121 taaagacaag tatgcttatg tcgcagaatt tgatgaaatt gtggagaatg attacaactt
     3181 aaacattccc cgttacgtgg atacctttga agaagaaga
//
LOCUS       DACIFS010000119         3202 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.128, whole genome shotgun sequence.
ACCESSION   DACIFS010000119 DACIFS010000000
VERSION     DACIFS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.128"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            671..766
                     /locus_tag="IYA83_002580"
     CDS             671..766
                     /locus_tag="IYA83_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ8587967.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1068..1313)
                     /locus_tag="IYA83_002581"
     CDS             complement(1068..1313)
                     /locus_tag="IYA83_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587968.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1658..1942
                     /locus_tag="IYA83_002582"
     CDS             1658..1942
                     /locus_tag="IYA83_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587969.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1945..2361
                     /locus_tag="IYA83_002583"
     CDS             1945..2361
                     /locus_tag="IYA83_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587970.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2498..3175)
                     /locus_tag="IYA83_002584"
     CDS             complement(2498..3175)
                     /locus_tag="IYA83_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ8587971.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          598 c          499 g         1040 t
ORIGIN      
        1 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
       61 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
      121 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtactcagat
      181 aaaaagttgt tagtaccctt ctgtatccgt tgggagagta aggctcaact gccccctaaa
      241 aagggttcta aaaaatcttt aaaaaataaa taaaataaac gcgcgcgcgc gagcaaaatt
      301 tcccgccaaa aaatcaccca ctttttaaaa taaacaggtt cgcgcatgat attaaactgc
      361 caatctagct ccctatggtc gctgtaagaa ggagtgatcg ctacgctctc acattttact
      421 tgcagcagtc gcctttaggc tccttcgcca aaaataattc attagggact cctttgtggc
      481 gtcctgagag ctcgtctaag ccgttctaag acaagattcg ataaaaacta tgtccttcgt
      541 tctaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccccaaggca ataaaaaact
      601 taggcttatt tttgcttttt tattgtataa ttgattgtcg cctatccgac agtcttgcga
      661 ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc tcccttcttg ttgggctttt
      721 aagcgctttg ttcagttact ggttgaataa ccgaaacaag aagtaaaact taattaaggc
      781 gactcagccc acaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtgtttc
      841 gtgttcactt atttgcaatc aaattataac agaaataaat atttatttca aaaatatcct
      901 acaaaagtct aaaatccgta attatttctt atatgcaaca tttacaaata aagtcgctct
      961 gagagctcgt atttttcgat ttaagacgtt ttatttagtt tttggtgtat ttcattggtc
     1021 caagctaaaa aactcttaga agcgatttta gagccattta aacccactta attatattct
     1081 ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa ttccgccgtc ttcgttcact
     1141 agttgcatta atttttccaa cgatcgtttt ctttcgggtg tcatattttt atattccaac
     1201 aatttttcgt ctgttaaatc gaacttgatt gcttgaattg cttgctctaa tttttctaag
     1261 ttttgaaaat attcgtaagg aaatttttcc attttttctt tctccagtct cacaattttt
     1321 ctagaaataa aatcacttct ttgccgtcta tactctcaaa aaaaatgtcg tttgaaaaac
     1381 taaataaatt tttgatcgtg catttttaaa tttggttatc gattacacgt gcagcactcc
     1441 cccccgaatt ttaaagagca taaggaaacg tgtacccact ttctaaaaaa agtgggtaga
     1501 taggctgtgc ctatcacgtt tctcagaaaa aaatttgata tcactttttc gccatcgtgg
     1561 gtagcttatg tatagcattt tggcaaaaaa cagctataca aatatagtat ttgtatggta
     1621 tacataaatt atacataatt gcaaaaggag attttttatg gacattttag ttcggaatat
     1681 tgatccaaag tttgttgcaa caatagacaa acgttgccaa gaactcacga ataaaacagg
     1741 agaaaaatgg agtcgaaata aatatctaaa agttttaatc gaaagtgatt ttgaccgtca
     1801 acttacgact tacaaaaaag accaattcga tttattgctc gataaattta ttgctatcca
     1861 aacagaaaat actcaaatat taagcgagta tgtctacaca aataatcaaa tcattgaaat
     1921 gcttatcgga ggaatgatat aaccatgaga aaaacttctg tccaaaaaca tgttactttg
     1981 taccctagtc atgttgacgt tttagattcc atcatcgaaa aaaatccaga tatcaataac
     2041 tactcggaag ccattcgctt tttatgtatg agttatgaac ccgataccaa cacgaccact
     2101 accagcaatc aggcaaaact aaatgctatg ggaaaagaaa tttccatcat gactgaaatc
     2161 atcgcagaat ttgctgattt atatctaaaa gatacaggcg ttcttattgg cacaaattct
     2221 aaagtatatc aagaagtgaa agaaaaaatc gaagctaaac taaatccatc aaatcgaaat
     2281 cgaccaaaga atcatcaaaa agaaaccagt caaacagtca cttctagaaa tttcgaccca
     2341 acaaattttc taaaacgata aaaaacagat aggagttatt cctatctgtt ttttatttag
     2401 aagccccata caatatgcta gaatgtacta gcaatcttag tcggttgtaa gggacttgtc
     2461 tacttgctta atgtaagggg ggcgagttct tttttattta ttcttctaac cagttacgca
     2521 gagaaatctt tggcaaatct gacgttaggc ttgaaaaagt aaattctaat ttggcgattt
     2581 tattgccctt ttttgctttt atttttgtga ctttaagatc attgaagtat ttttttagtt
     2641 ctcgtaatgc aggatttaat actttttgat caatgttacc catttgatag tattctggga
     2701 tatccattaa gctcctaaac tcttcaatac gaactacata ataaccagta gaacggtact
     2761 gcataagcag tcggaataga gtttttgcat acgtagaacg caacgacgtg aaagcagata
     2821 attcaaatct actaaattca gctgttaaac cattaataat atattctagg tccggattaa
     2881 cactgacttc aacaaacttt tttgttttat tgatttcaaa accagtaaac aaaacaaagt
     2941 agcgaataga atcctcatct tcagtacggt aagtaagatt aagcatatct ttatagagct
     3001 tttctaaatc accgacaaag gcttttgtag cagtcatctt ataatcactt aactctttta
     3061 attcctcgaa agaaaatcta acttttttca aaccacgatc tttcatacga gcgcataatg
     3121 caaaaaataa atccatctgc ttagcattga aatttttcag cggtactaaa ttcaattcat
     3181 tttgatattt gactgttaat tc
//
LOCUS       DACIFS010000120         2999 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.130, whole genome shotgun sequence.
ACCESSION   DACIFS010000120 DACIFS010000000
VERSION     DACIFS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.130"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..586)
                     /locus_tag="IYA83_002585"
     CDS             complement(<1..586)
                     /locus_tag="IYA83_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587972.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="IYA83_002586"
     CDS             complement(1224..1481)
                     /locus_tag="IYA83_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ8587973.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="IYA83_002587"
     CDS             complement(1474..1743)
                     /locus_tag="IYA83_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ8587974.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1874..2053)
                     /locus_tag="IYA83_002588"
     CDS             complement(1874..2053)
                     /locus_tag="IYA83_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587975.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(2434..>2999)
                     /locus_tag="IYA83_002589"
     CDS             complement(2434..>2999)
                     /locus_tag="IYA83_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587976.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT          914 a          562 c          396 g         1127 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
     1921 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
     1981 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
     2041 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
     2101 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
     2161 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
     2221 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
     2281 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
     2341 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
     2401 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
     2461 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
     2521 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
     2581 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
     2641 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
     2701 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
     2761 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
     2821 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
     2881 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
     2941 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttctt
//
LOCUS       DACIFS010000121         2921 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.131, whole genome shotgun sequence.
ACCESSION   DACIFS010000121 DACIFS010000000
VERSION     DACIFS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.131"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            7..729
                     /locus_tag="IYA83_002590"
     CDS             7..729
                     /locus_tag="IYA83_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ8587977.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            787..2826
                     /locus_tag="IYA83_002591"
     CDS             787..2826
                     /locus_tag="IYA83_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ8587978.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         1038 a          515 c          625 g          743 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgacagt
     1861 ctaggaggaa aagctggatc aactgttgcg acaacgccaa aaaccggtga tcttcttgcg
     1921 cttgctagct ctccaagcta tgatccaaac aaaatgacaa acgggatctc acaagaagat
     1981 tacaaagctt atgaagaaaa tcctgaacat ccattcatca gccgatttgc gacaggttat
     2041 gctcctggat ctacgtttaa aataattaca gcagcaatcg gtctcgacaa cggcactatc
     2101 gatccaaatg aagtgttgac gatcaacggg cttaaatggc aaaaagacag ttcttgggga
     2161 tcttttcaaa caacgcgtgt cagtagtgat gtatcacaag tagacttaaa aactgctttg
     2221 atctattccg ataatatata tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt
     2281 cgtacaggct tagataaatt catttttggt gaagaccttg atttgccaat tagtatgaat
     2341 ccagcacaaa tttctaatga agatagcttt aactcagata tcttgctagc tgatactgga
     2401 tatggacagg gcgaacttct aattaatcct atccagcaag cagcaatgta ttctgttttt
     2461 accaacaatg gcacacttgt ctatcctaaa ttgattgcag ataaagagac aaaagataag
     2521 aagaatgtca tcagcgaaac agcagtacaa acgatcgtgc cagatctgag agaagttgtg
     2581 caagatgtaa atggtacagc acattctctt tctgctttag ggattccgtt ggcagcgaaa
     2641 actggtacag cggaaatcaa agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg
     2701 tttgctttca atcttgataa ccaaggttat atgatggtca gcatgttgga aaataaagaa
     2761 gatgatgatt cagcaaccaa acgagcatcg gaactattgc aatacctcaa ccaaaattat
     2821 caataaaaaa ttgcttttta agtacagtaa aaggactgtg gcgttatcgt cacagtcctt
     2881 ttttcaaata aacggttttc acgatgtctc tttcacaact t
//
LOCUS       DACIFS010000122         2593 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.132, whole genome shotgun sequence.
ACCESSION   DACIFS010000122 DACIFS010000000
VERSION     DACIFS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.132"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..1152
                     /locus_tag="IYA83_002592"
     CDS             <1..1152
                     /locus_tag="IYA83_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587979.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IYA83_002593"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IYA83_002593"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ8587980.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          859 a          491 c          620 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca act
//
LOCUS       DACIFS010000123         2559 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.133, whole genome shotgun sequence.
ACCESSION   DACIFS010000123 DACIFS010000000
VERSION     DACIFS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.133"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..902)
                     /locus_tag="IYA83_002594"
     CDS             complement(<1..902)
                     /locus_tag="IYA83_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ8587981.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IYA83_002595"
     CDS             1185..2153
                     /locus_tag="IYA83_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8587982.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IYA83_002596"
     CDS             2266..2544
                     /locus_tag="IYA83_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ8587983.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT          837 a          466 c          524 g          732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaa
//
LOCUS       DACIFS010000124         2483 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.135, whole genome shotgun sequence.
ACCESSION   DACIFS010000124 DACIFS010000000
VERSION     DACIFS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.135"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(1014..1304)
                     /locus_tag="IYA83_002597"
     CDS             complement(1014..1304)
                     /locus_tag="IYA83_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587984.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IYA83_002598"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IYA83_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACIFS010000125         2469 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.136, whole genome shotgun sequence.
ACCESSION   DACIFS010000125 DACIFS010000000
VERSION     DACIFS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.136"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..418
                     /locus_tag="IYA83_002599"
     CDS             <1..418
                     /locus_tag="IYA83_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8587985.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR"
                     /locus_tag="IYA83_002600"
     CDS             632..1327
                     /gene="vanR"
                     /locus_tag="IYA83_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ8587986.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="IYA83_002601"
     CDS             1305..2459
                     /locus_tag="IYA83_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ8587987.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          852 a          443 c          521 g          653 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtata
//
LOCUS       DACIFS010000126         2422 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.137, whole genome shotgun sequence.
ACCESSION   DACIFS010000126 DACIFS010000000
VERSION     DACIFS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.137"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..290
                     /locus_tag="IYA83_002602"
     CDS             <1..290
                     /locus_tag="IYA83_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587988.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="IYA83_002603"
     tRNA            complement(803..876)
                     /locus_tag="IYA83_002603"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="IYA83_002604"
     CDS             complement(1065..2084)
                     /locus_tag="IYA83_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ8587989.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="IYA83_002605"
     CDS             complement(2095..2301)
                     /locus_tag="IYA83_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ8587990.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          811 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tc
//
LOCUS       DACIFS010000127         2398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.138, whole genome shotgun sequence.
ACCESSION   DACIFS010000127 DACIFS010000000
VERSION     DACIFS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.138"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            207..2069
                     /locus_tag="IYA83_002606"
     CDS             207..2069
                     /locus_tag="IYA83_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8587991.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IYA83_002607"
     CDS             2069..>2398
                     /locus_tag="IYA83_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8587992.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACIFS010000128         2388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.139, whole genome shotgun sequence.
ACCESSION   DACIFS010000128 DACIFS010000000
VERSION     DACIFS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.139"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(493..1785)
                     /locus_tag="IYA83_002608"
     CDS             complement(493..1785)
                     /locus_tag="IYA83_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ8587993.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IYA83_002609"
     CDS             complement(2059..2319)
                     /locus_tag="IYA83_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587994.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          788 a          448 c          360 g          792 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaa
//
LOCUS       DACIFS010000129         2364 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.140, whole genome shotgun sequence.
ACCESSION   DACIFS010000129 DACIFS010000000
VERSION     DACIFS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.140"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            150..1451
                     /locus_tag="IYA83_002610"
     CDS             150..1451
                     /locus_tag="IYA83_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ8587995.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="IYA83_002611"
     CDS             complement(1555..1755)
                     /locus_tag="IYA83_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ8587996.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          768 a          453 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataa
//
LOCUS       DACIFS010000130         2293 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.141, whole genome shotgun sequence.
ACCESSION   DACIFS010000130 DACIFS010000000
VERSION     DACIFS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.141"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(51..1598)
                     /locus_tag="IYA83_002612"
     CDS             complement(51..1598)
                     /locus_tag="IYA83_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ8587997.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYA83_002613"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYA83_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ8587998.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IYA83_002614"
     CDS             complement(2043..2237)
                     /locus_tag="IYA83_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8587999.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACIFS010000131         2274 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.142, whole genome shotgun sequence.
ACCESSION   DACIFS010000131 DACIFS010000000
VERSION     DACIFS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.142"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..552)
                     /locus_tag="IYA83_002615"
     CDS             complement(<1..552)
                     /locus_tag="IYA83_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588000.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQN"
     gene            complement(683..1405)
                     /locus_tag="IYA83_002616"
     CDS             complement(683..1405)
                     /locus_tag="IYA83_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588001.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(1411..1656)
                     /locus_tag="IYA83_002617"
     CDS             complement(1411..1656)
                     /locus_tag="IYA83_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588002.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            complement(1667..2251)
                     /locus_tag="IYA83_002618"
     CDS             complement(1667..2251)
                     /locus_tag="IYA83_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588003.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
BASE COUNT          668 a          412 c          304 g          890 t
ORIGIN      
        1 attttgcact aaatcttgaa tcaatttcaa tctatccact tttttcatct catacagggc
       61 aatttcagct tgtttaatgc cttttaattt gcgaaaggca tatttgtatt gtgtgtaggt
      121 taaaaagcct gtgtagttat acatgggctt tcctgatgtc ttgtagtcat ttaagcttgg
      181 atatatttca gttttccatt tttcttctga acgttgatca ataattctta atttttcagg
      241 taaatcaatt tcatagtatt cttcaatttt cagctcacga taaagtagat gtttttcctt
      301 ttctttttca attacaaaag aattatttcc cttcagttta gctatttctt cacttgtgag
      361 accaagccta attttttcta cttcacttct aagttcacga atacttgttg cctttaacca
      421 cttcccattt gcaccattat tgtaaccggt aagtaactgt attttataaa ttccttcacc
      481 atgaagcacc ttatatatac caacctgatt cttttgcgga atatgagctt catttgcact
      541 gatttctttc attacgcatt ctcctttata atctagttaa ttagcttgtg tgtttcgagt
      601 agttgagtaa gaacgtttgt ttcagaaatc atttatttgt tatcacttaa ttttaaaaaa
      661 atagggctta cgccctaaag atctatttaa ctttggattg atcgtccata acaggctctg
      721 attgttgata taatttatat gctcccaaat ccatataata tttagttgta ctttcaatca
      781 tttcatcagt tgtatttgtt gtcattccca ttacatccca gtatagtgtg ccgttaactt
      841 tctttggtgt tactactgca actgttggcg tatgatattg attgttctca tcagccagac
      901 caggaatgtt ttgtacccac tcaggtacac catcagtaat atctacagca actgcgtaat
      961 actcgtaagc tggtttattt atggtaatat catgcattaa tgttggagca taactaaatc
     1021 cttcttttat ttcttgcatg atttttgatt ctgctttttg acaatctgga caatcaggtt
     1081 tgtaaagaac gatagtcaga tttttatttt gcaatggatc attcccataa ttttcatcat
     1141 tttttactgt attaataaag ttgttataaa cttcatccgg cttttgacct ttattaatgt
     1201 cttcgtattt ttgatacttt tcagcattat tttcagtcat tgaagacatt gttgttgatg
     1261 ccgatgtagt gctgttttta gtaaccatcg tactactaga ctttgtgtct ttgttattat
     1321 tagcactatt agttgtacca catgctgtaa gggttattaa tgctgctgtc gataacaata
     1381 caaccttcaa tttagttgat ttcattttat tcacctatcc ttttttcttt tgtttaacaa
     1441 taaaagaata acccgtacta tccaataaaa caacagcatc aatatggaaa cccaaaccat
     1501 ttgtacaatc caagcataaa gactaaataa taggtattgt ggattttttt gaatgattgt
     1561 atttgagatg ccatttccta cagtaaaata cattgttacg aaaataacac cttcaattat
     1621 caagtgattt gttggttgat ttataatttt tttcatattt ttactcctaa actaaattta
     1681 ttcttcctgc taaatattga aaattatatg ggttctgcac atattcccta agttccttaa
     1741 tacttgtttc aatatcacca aaggctgttt taatgtttgc tgacccatct ttatatacaa
     1801 tcatttcccc ttcacccata caaaaagaaa tttcagtatt agaattgatt gttttttcag
     1861 cacgaccatt gataatttct tgaccatcac gccatataaa atacataaga taaaatacct
     1921 cataagcgtt gtatccagta tccgttgtca ttgataccaa aacatcagca tcttgtattc
     1981 ctgataaagc taatccaatt tcatggatca catcaacttc attttcttcc caatctccac
     2041 tttcgaagac ggtataggtt tgattgttag caaatggttt tctttctata tcgaacattc
     2101 ctttccaatc aagaatcatg tacccgatta aattgtgaaa gaaaattaga taaaataaat
     2161 acaataagat gctaactaca aaaatatcag ccattattga cataggatta tcaggatata
     2221 accagatgta aaacgattgt ttcaaatcca ttttttcatc atctcactct acat
//
LOCUS       DACIFS010000132         2192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.143, whole genome shotgun sequence.
ACCESSION   DACIFS010000132 DACIFS010000000
VERSION     DACIFS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.143"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..468)
                     /locus_tag="IYA83_002619"
     CDS             complement(<1..468)
                     /locus_tag="IYA83_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ8588004.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ"
     gene            complement(1030..1233)
                     /locus_tag="IYA83_002620"
     CDS             complement(1030..1233)
                     /locus_tag="IYA83_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588005.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(1692..>2192)
                     /locus_tag="IYA83_002621"
     CDS             complement(1692..>2192)
                     /locus_tag="IYA83_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ8588006.1"
                     /translation="QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQE
                     TKRKIVPPEIKTWWRLELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIA
                     GLISDQNLWAGLARHTKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLG
                     LDVTEE"
BASE COUNT          629 a          506 c          347 g          710 t
ORIGIN      
        1 ttgcgtaata aaatcatcag gcacatcgat aatgtcgcaa gcaatatcag cacgtgagaa
       61 atgtggttct aaaaataaat catggataaa gttcttcatc gaacccgcca aaaattggct
      121 aattttattt gggttaaagt caattcgccc ttgcccttct tgatatttca aaatttcaat
      181 gtaagcaata ttctcatcat gcttatccac aatttgccaa ccatttagac caacagattt
      241 aacataacca ttattttcat tcagtcgcat taactgttca aagtctaaaa tcaatacatc
      301 attttgcgtg tgataataaa tattctcttt gagttttcca acaatggtaa ttcggtcaat
      361 tgaccactgt attttaaggg gggtggtcaa agtttgcaca cttaaccgcc tgttagatag
      421 ggctgttaaa ttttcccact catttatgat cgtgccacgt ttactcaaat tttgttcctc
      481 ctcaaagaaa aagagacttt tggaaagaat ttccagagtt cacgtaaccc taaaaattca
      541 tatccaaaaa tctctagctt gaagaaacct aaaaatattc gtataatgaa cctgagttac
      601 ggagctaggt gaagtagttt ccgtaaatag tcgagagtgt tagcgcactg ctcggctttt
      661 attttgctct tttttctcgt tttctttgat tatagcactc ctcctaaaaa aataaaaaga
      721 cctcagggaa tttcattccc cgaacccctt gagcctcctc ctgccgaaat ggaaagggcg
      781 cacccgctgt ttatttcatt cagctatcgg ctttcacaat ctaacacgat cgttccgcta
      841 ccagtcgcct tacaggctcc attttcgctc ctctaacgtg ttttaacgat tgtgccgata
      901 actaaacgaa ataaacgcta aaaccgctca gaacgcaaaa tatgccattt tagagctatt
      961 gtagaaaatg tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa
     1021 atctgctaat tattttctac cagacaaacc attgattttt tggctatcag ttaactcacg
     1081 attattatga gaaatgcttg tattctttgt tccctttttg aacgaaatac taaaccctaa
     1141 ggtgaacccc tccttcgcgc tgtcgctaat aaaaagagtg taaacctttc tgttactctt
     1201 tgacaaagag taacattttc ctaattgtga catggctgtc acaattccaa acgctctccg
     1261 agggttttcg ctgtcacaag tgacaacgaa aaaaggagcc gaaagcgtgt cacgctctca
     1321 actcctttta cgctttattt tgcttcacgc aaaataatcg cttatcttaa acattgacgt
     1381 aaatttactc tcacttatct gactttttta ctcggtatca ctccgagtaa gccgtcatat
     1441 tcattgggat aatctcgtct gcttcgctct aattttccag agtgaggggt aactccttcc
     1501 cccgaattac cccaaagagt gacactggga tatccctaaa tttattataa aaagccaagt
     1561 ctctttgcgc aactgtttta tccaagtcgg catttgcgga ttcgttagcg ttttttcgct
     1621 cttgctgcaa acgcacgacc caccatgccg acttgtgctg aaaataaaaa agtggacaaa
     1681 aattcatcca ctcattcttc cgtcacgtcc aaccccaaaa gccacgtatc caattctttt
     1741 ttcaaaacat ctgccgattc cgaaaatgtc tctcgtaaat gattaactaa ctcatcactt
     1801 tggctttctt ttttcaacaa atttcgatat ttatattttg tatgacgagc caagccagcc
     1861 cataaatttt gatctgaaat caatcctgca atcataattc tatcagtatg ctttgtatca
     1921 gtcggcaaaa aatgaggact tgcgaaactg tctaaacttt catgaaccat cgcataccaa
     1981 tcagtagctt ttccacgccg taactgcaat tccagtcgcc accaagtttt tatttccggt
     2041 ggtacaattt tccgtttggt ttcttgttcc aattttttgt tgtacatcct tatttgtcgc
     2101 tcactggcac gactacccca atatgccgtt tccagcttcc cagaccgccc atagattggt
     2161 ttgaatgaaa cagggtcaac cacacgatat tg
//
LOCUS       DACIFS010000133         1970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.144, whole genome shotgun sequence.
ACCESSION   DACIFS010000133 DACIFS010000000
VERSION     DACIFS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.144"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..85)
                     /locus_tag="IYA83_002622"
     CDS             complement(<1..85)
                     /locus_tag="IYA83_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8588007.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            313..855
                     /locus_tag="IYA83_002623"
     CDS             313..855
                     /locus_tag="IYA83_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588008.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..1460
                     /locus_tag="IYA83_002624"
     CDS             1011..1460
                     /locus_tag="IYA83_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ8588009.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1490..>1970)
                     /locus_tag="IYA83_002625"
     CDS             complement(1490..>1970)
                     /locus_tag="IYA83_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ8588010.1"
                     /translation="KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          702 a          303 c          372 g          593 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccaga tggaatggca acactttttt
     1381 tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat ggctccgatt gcccttgaca
     1441 atgagcatcc tcggatctga ttttctccgg cagaagcaca atcattcaat tacgctgcct
     1501 ctccagaggt atcagatccg cgctcattat caaggattcg cttcgccaat ctctgttgtc
     1561 gaatttggat cggtgtttca tacgccaacg tgccatgtaa gcgtagatag ttccaccaat
     1621 gcacataatc aaacaattct agacgtagct gtgccagtgt ctcaaattgg tattgatgca
     1681 cgaattctac tttgacagat ttataggttg attccacaac ggcattatca tacggacaac
     1741 ccttcctact caatgaacga gtaattccaa aaccattcag aatctcatga atggtttggt
     1801 tatcaaattc ctttccacgg tctgtatgaa aaagcttgac gtctgttaaa gaatacggta
     1861 tccgtccaaa ggcttctttt accaatgagg catctttctt ctcaccacag gaataaccaa
     1921 taatttctcg attaaacaaa tcaagtatta agcagatata atgccacttt
//
LOCUS       DACIFS010000134         1880 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.145, whole genome shotgun sequence.
ACCESSION   DACIFS010000134 DACIFS010000000
VERSION     DACIFS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.145"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            122..1276
                     /locus_tag="IYA83_002626"
     CDS             122..1276
                     /locus_tag="IYA83_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ8588011.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1270..1692
                     /locus_tag="IYA83_002627"
     CDS             1270..1692
                     /locus_tag="IYA83_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588012.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          705 a          297 c          286 g          592 t
ORIGIN      
        1 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
       61 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaaga
      121 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      181 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
      241 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
      301 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
      361 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
      421 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
      481 agaatattca ggaagtatct cacaattttt agcaaaagaa cttcaaaatc tagtagatag
      541 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
      601 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
      661 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
      721 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
      781 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
      841 agaagctgct atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat
      901 gcattatacc tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga
      961 tcaaataata aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact
     1021 aaacctttca gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc
     1081 tggttatgga aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca
     1141 aggtggttat caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag
     1201 tgcaaatgca ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac
     1261 cattacaaat tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc
     1321 ttaactagtt gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga
     1381 acttggcaaa ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa
     1441 aatatagaac aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat
     1501 aaaaatttaa atataaatga acaagttttc atttacaaag caacaaataa tttactaaca
     1561 ctgcgctcta ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc
     1621 gtatttattt tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt
     1681 aaagctgatt gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa
     1741 caagcgaaac tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt
     1801 gggttttgag ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc
     1861 aaatacggag gtatttggct
//
LOCUS       DACIFS010000135         1878 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.146, whole genome shotgun sequence.
ACCESSION   DACIFS010000135 DACIFS010000000
VERSION     DACIFS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.146"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            240..851
                     /locus_tag="IYA83_002628"
     CDS             240..851
                     /locus_tag="IYA83_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ8588013.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YMNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKN"
BASE COUNT          678 a          257 c          315 g          628 t
ORIGIN      
        1 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
       61 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      121 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatcggtt cttttgcaaa
      181 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa
      241 tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct gttggttact
      301 acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat cgtggaataa
      361 atgtttgtca tactacgatt tatcgttggg tacaagagta cagcaaagtc ctctattatc
      421 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
      481 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
      541 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
      601 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      661 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      721 atatgaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      781 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
      841 aaaagaactg aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat
      901 taatgggaat aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac
      961 ttcgcaacag aacccattag caatatataa aagaaggtat caagagtcac agtgaattta
     1021 ttccgaaaag tgaaaagagc aaatataaaa tttaataaaa ataagaacta aataggaaaa
     1081 cgtagacgta attttctacg ttttcctttt catatatatt aaatattacg gaaaattata
     1141 taaactaatt tagtgttttg tttgtaattc ctaattttgc tccactttta gataattttt
     1201 aggtctattt aactcattag acattctatg ctcatttttc tccaaatttg tttcaactct
     1261 ttcgtactta attttgcgta tttgaaaatt cttcttttag agtactttta ttacgatact
     1321 agtatgatat atatctacag caaacagtta aaatcttcct aaaataagcg aatagcattc
     1381 ctaaaaccta taaaattttt agatgaagcg acaagtggac aattaataaa ctacttgaac
     1441 atgaaattat ggatcacctt tcaaatttag aaaatggaat tcttatactt attgcgcaac
     1501 atttattgat tgcaaaatta tgtgacaaaa agaactagta gaagaaggaa taatggcttg
     1561 ataataaagt actacctatt aaaggtagta ctttatttaa tttagtataa attaacatac
     1621 tactatattt taggtttcat attatataaa ttaataatat actaccattt gttggtagta
     1681 taatttaaag gtaataagtt attcttttat tactatgttt gggttttata gcaaagaaag
     1741 tttaataaga gtttggacct agcaaacaat attaatgagg tcattaaatc ttattatctt
     1801 tagtgatata atgaaatatc tacacgacaa aattatgatc ttgcgtaaag ttataaaata
     1861 ttaataaaaa tattttat
//
LOCUS       DACIFS010000136         1822 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.147, whole genome shotgun sequence.
ACCESSION   DACIFS010000136 DACIFS010000000
VERSION     DACIFS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.147"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            367..921
                     /locus_tag="IYA83_002629"
     CDS             367..921
                     /locus_tag="IYA83_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ8588014.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            1175..1486
                     /locus_tag="IYA83_002630"
                     /pseudo
     CDS             1175..1486
                     /locus_tag="IYA83_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1597..>1822
                     /locus_tag="IYA83_002631"
     CDS             1597..>1822
                     /locus_tag="IYA83_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ8588015.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVC"
BASE COUNT          720 a          212 c          316 g          574 t
ORIGIN      
        1 atgaaaagtt tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
       61 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      121 tctaagaact tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca
      181 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      241 tataaaatca taaactaaac cccttgattt tcttcacttt taacagagta tatatataag
      301 ttcatgatta tggtaaaata tatatataat catgaactca gaatattttt aagaagggaa
      361 acaatagtga gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag
      421 ttttggctaa gaagaacaag taatccagaa agagatagtt ttttattttt gtttggaatc
      481 aataatgggt tacgtatgtc agatattata ggattaaaag ttggagacat acgaggtaaa
      541 agtaagccta ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat
      601 ttgagagaag agattttatt atatactgaa ggtaaagaag aaaatgattg gttatttcca
      661 agtcggcaac aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata
      721 gcagaaaaat taggaagaga tgatatagga acacatacat taagaaaaac ttttgggtat
      781 cactactaca agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg
      841 caagcaatta caaaaaggta cattggtata actgaagatg aaataggggc atcgttacgt
      901 ggttttaaat tgggaggcta attgagatat taggatatat tttttgtgta taatgaaaac
      961 ttagaaggtg agattaatga agataactga attttcggaa gtatttaaaa cgtttagtaa
     1021 catttacaat agaataccag agatagaaca aaatgttgta gttgataatg attattatat
     1081 agagaatgat tttaataaac caagaaaata tgacaaccta tacaatttta tacacaatag
     1141 aacaacaata aataacaaca gtaacactcg agttttgatt aattcagcat attttcatat
     1201 taaatattta aagaaatatg agtatgaaga agttgtatta gattatgggc taaatagaca
     1261 gtcattttta atttatgcaa taaataacgg aaatttgcca agcaataaaa agaacataat
     1321 tattgaaaca tatgcctatc tggagggggg atctaaaaaa ctaattaagt catgtaatat
     1381 agagtatcaa ttatcagtag gagaaattaa atgtgttttc gaagaagatt tagaacttga
     1441 cttaaaaaaa atttttgcca gcattcatga ttatataggt ttgatacccg tggtgctagt
     1501 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcatat
     1561 agtagtctca aaactcccaa gaaatgagga ctacatatgc gttcatcaaa atatactgaa
     1621 cattatacag atacttttat aacattcaag gaaattatga gaactgtttc taacatatac
     1681 cacaattgtg taccagataa gattaaaaac cgaagaaata ctgatcaact gaagcaacgc
     1741 gatacagtga ttattgcttg tgttatatgg ggcataatta atggctatac tagccaaaga
     1801 gccacgtata gagcggtttg tt
//
LOCUS       DACIFS010000137         1720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.148, whole genome shotgun sequence.
ACCESSION   DACIFS010000137 DACIFS010000000
VERSION     DACIFS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.148"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(30..578)
                     /locus_tag="IYA83_002632"
     CDS             complement(30..578)
                     /locus_tag="IYA83_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588016.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(579..1436)
                     /locus_tag="IYA83_002633"
     CDS             complement(579..1436)
                     /locus_tag="IYA83_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ8588017.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          483 a          347 c          247 g          643 t
ORIGIN      
        1 aaggtagtta cttagttctt ctttgattat tatctaactt tattattcat tccccaccct
       61 ttgcgcagtg agtcttcggt atcttgtttt atctgactgg tagttcttat tatttctcca
      121 cgatcatcta attctggaac aattggatat ttttctctga acaagtctag ttctttcacc
      181 acgtcattaa acaaatcttg cttatcttct tgcgtaagat tctgtttaat atactcatct
      241 aaagcttttc taagcaattc atctcttgtc atattagtct catacttttc tactaaaact
      301 ctggaagtcg catttaacaa agtcgacagt tttaaaggat gcttggtgga ataaacctta
      361 tcaaactttc tgattggtgc gccaactttt tttgttcttc ctctactctg tctttcaaca
      421 ttggaggaag tttttatttc ttgaacttgg tgcacttcta attgcttatt ttcagatggt
      481 gtaattgctt gctgcttttt ctttgcttct gcttcttttt ttattttggc gagtccttta
      541 ttgttcccaa aaatatcaag tcctttgatg tcagccaatt actccacctc cgcataccaa
      601 ttctcgtgtt cttccaattc tttcaaaata ctgataaata attcgtggct tttgcgatcc
      661 cattgtttta agagaccttt ctttgtcttc tccatcgtaa tacctgatcc gtcgtatcgt
      721 tttaagcgcg catttttgag tacaacattt ttaatcaaat gttccccata aatctcttgg
      781 gcttgtttat acatttcctg atctactgcg tcaccgcttt ccaacatgaa aggaataaca
      841 ccagcaacat ataaatttgt atcaaattct ttcacaaaaa agtccatata ctcaagatat
      901 tgcatcgcat ttctcatcga ttttacttgg gtctgtaaga cgacgattgc ccagtccgca
      961 atgtacatcg cggatttact atatacagaa atagaaggtg ggacatctaa atagatgacg
     1021 tcaaactcat caaataatgg ttctagcttt tctttcatat atttgaattg ggataatgaa
     1081 tcattatatt ggtctttgat ccaatcatca tacatttcaa aggacatgtc tgctggaagt
     1141 atatagaggt tattgattac tttatagaca gcctctttag gtggaagatt gtttatcaac
     1201 acgtcagtca cagtattttc gtaaacaatc ggttcacctt ctccgcctcc ttcttcgtta
     1261 gtgaagttca tatgcgttaa gttcatcaca tcagaagtat ttccttgcat atccaagtca
     1321 attatcaagg ttctttttcc ttcattaact gctgattcaa accctaacat ttgcgcgcac
     1381 gtacttttac ctacgccacc tttaaaatta ccgattacat atttcttcgc taccacgttc
     1441 ctacctcttt tcttttagca atattcattt gatactatta taacatattt atttttagtt
     1501 actaccaaaa aaaagatata tatgtactac cttataatta ataaggaaaa ggtagtacat
     1561 aaataaggaa aagctgaaat atattgattt atagctttcc tcaaagtaat gacgttattt
     1621 aatttatcga gaagcagaga atactcgcga ctttagtcat gtagacgaat cagattttcg
     1681 ataaaaaaga agccgtcctt cttaagaacg acaacaaatc
//
LOCUS       DACIFS010000138         1719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.149, whole genome shotgun sequence.
ACCESSION   DACIFS010000138 DACIFS010000000
VERSION     DACIFS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.149"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            386..574
                     /locus_tag="IYA83_002634"
     CDS             386..574
                     /locus_tag="IYA83_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ8588018.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            652..1665
                     /locus_tag="IYA83_002635"
     CDS             652..1665
                     /locus_tag="IYA83_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ8588019.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
BASE COUNT          576 a          278 c          361 g          504 t
ORIGIN      
        1 tttagttagt ctacattatt ttggcgattt gccaataagg actgttgagt tgtgcaaaat
       61 caaatctggg tcagaatgaa cgtcattctg tctcagattt ttcgttattt caatttgatt
      121 aactgattga tcaaaaagat ttttattttc atattctcaa atgattcaac actaaatata
      181 catgtttgac gcggaaatta ttattcgatt ttggcggcat caggggggat gacgacgtag
      241 cagacccatt attttactca agaaaatgtc cgtgcctatt atttagaata cgatagtgaa
      301 cgtgctggga gttttgttct tttagagaaa gtctctgaag ataaattagt tgtcttagat
      361 tttgactgta acgaaaaatg gcgaaatgga agagcaccag caagtgattg accggatcta
      421 cgaagcgaca cgatatctgc ctttggatcg tttgtgctta agtcctcaat gtggatttgc
      481 ttcaacagaa gaagaaaatg ttttgtttga agaacaatgg aaaaaatttc ttttgtcaaa
      541 agcattgcag aagaagtttg gggaaaatca ttaaatcatc taggagagct tgataggaaa
      601 taacactatg gtataatcag aatgcttaat actaactact gagggagcct tatgaaaaag
      661 aaaattctta aatttatcgg atggttcttc ggaatcattc ttgggatcgc gtttgcaatc
      721 tttcttgcct tccagctttc ccccagacct ggagcattct tgatcaacca tatgttttca
      781 aatactgttc aaatcactga taaaaaaacg ttcgatcaag cagaaaaaaa cgtggcgaaa
      841 aaaacagatt tgacgtatga gtccaagtat aaagagaata cttatgacat ctactatcca
      901 aaaaacagca aggggcctgt tcctgttcta ttttgggtac atggtggagg attcgtcggc
      961 ggagataaat cgggtgtcaa agaatttgcg acaaaattag tttcagacgc caacatcgca
     1021 gtggttgcga tgaattatga attagcgcct gattcagaat accctaatca agttcttcaa
     1081 gtgaatgaac tgatcaaaga tttattagct gaagaaaaag acgacaaatt gctagacatg
     1141 gaacaattat tttttggtgg agatagtgca ggttcacaaa tcgcattgca atatgcagct
     1201 atacaaacga atgatgcgta tgctaaacaa atgaaaatca aacagctact tccaaaagat
     1261 agtctgaaag gaaccatttc ttattgcgga cctgttgatt tgaagctaac agcacagcag
     1321 cattcggatg atcgatttat gaagtttttc gtcaaaaccg ttgcgtggtc gttgattgga
     1381 acaaaagatt ggaaaacgag tgagcaattg aaagaagcta gtgtggctga ccatgtttct
     1441 aaggaatttc cgccaacgta tataactgac ggaaatgcat actcattcca ggagcaaggt
     1501 caagcattag aaaatcggtt gacagaatta aaggtacccg tgcaatcctt gttttataca
     1561 gatacaaaaa aagagattac ccatgaatac caatttgatt acacgctaaa agaatcgcaa
     1621 aattgttacc agcagacatt ggattttgtg aataagtata agtaagaggt tgtgaaaaag
     1681 ccgtttcgtt ttgagtatga agaaacgaag cggcttttc
//
LOCUS       DACIFS010000139         1654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.150, whole genome shotgun sequence.
ACCESSION   DACIFS010000139 DACIFS010000000
VERSION     DACIFS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.150"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            56..856
                     /locus_tag="IYA83_002636"
     CDS             56..856
                     /locus_tag="IYA83_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588020.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            944..>1654
                     /locus_tag="IYA83_002637"
     CDS             944..>1654
                     /locus_tag="IYA83_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588021.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQ"
BASE COUNT          646 a          204 c          353 g          451 t
ORIGIN      
        1 tcttatttgt gaatgaaaaa caagtcatcg taaaatatta ataggaggtg tagtaatggt
       61 gggaatatca aaaacagctt tagactcatt agctaaacta tttagtagca ggtttgatgg
      121 tgcagaattt gttgaatatc taagccaaaa cgaaagtgga aaaactctga tcgaaacaat
      181 gaaaaaagac tatttgaatg aaaatgactt tccgacatgg acgaatattg aagatgctac
      241 atgggatgta gtatgtacga tatattctcc ttgggtaacg attgctgatt ttgttttaga
      301 taaaattgga ggggatacat accctgatgc caaatctctt gctacaagtg actttaaaac
      361 gttctatggt tatctaatag gtctaatagg caatggaata gatttgaaag ttacgccaaa
      421 acaaacacct gaagaaattg aaaaagaaaa aggagaggtt caaatggctt atgcaaatga
      481 cagagaaaaa gtaaaattat tggaaggtaa attatctgaa gaagaacttg gaaaaatggt
      541 gcaagaaatc aatgaagaca gcaactattt tcctgaattg aaggtatatc aaaatgacga
      601 agatttcttt aatgaaatgt ttagtaatag tccatgggaa gcagtgagta aaacaaattt
      661 aggagaatgg aactttcaag atgatctagt tgcatttagc aatgatgggt atttacgatc
      721 atattcagaa agtgagtata gagctgaatt aaaaacgtat gaaacggata ttctgatgag
      781 ctatttaaat ctattagaga gtggtcaggg agagttaaga gataaagttg aagaactatt
      841 aaaagaagag ctatagggat tatttcaaca taaacacaag cagaaaacta ctaaacaaaa
      901 cgtaccatgc aaaaagtcgg ttaaaccggc tttttgctct attatgagag gagatcaaac
      961 catgagtgca acaaccgata tggctttaga agcagtaata aaagataata caggaaatgt
     1021 cgaaaacgta gaagacttta gcgatattgc aatcaatctt tcagcatgga tttcagctgt
     1081 aggagaagaa aatatcgaat ttgagatcaa gaatatggat gacttaaaag caaaatttcc
     1141 ttttatcgca tcgaaactaa gtcaagttgc cgaggataaa tcgtggaatg aattagttag
     1201 tttttccgaa aaaatttatg atattacgca agaagatgag tgtgtttcat gggaaatgaa
     1261 taatcaatac aagaaaattg acgctttcgt caaatcacgt atagaaggaa cagcttttga
     1321 gtttgaaggt atagataccg cagataattg gttaaataaa gaatccttta ttaaaagtat
     1381 agcggatgat cttgaaaaac aatggtctaa ggaagactat gcagagtacc taaaaacaga
     1441 aagagataca gaggtgaaag aaaaactcac tgaggtatgt ttggatgaac atattgatcc
     1501 caatatcgta aaacaagaag actatgaagt ggttattgat aaagttccta ctgattttga
     1561 ggaagttgta aagtatctag ttgaagaagc attatcgcaa tactcttcag atgaggaagt
     1621 ggacgaagaa actgttcgta ccagagtttg gcaa
//
LOCUS       DACIFS010000140         1636 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.151, whole genome shotgun sequence.
ACCESSION   DACIFS010000140 DACIFS010000000
VERSION     DACIFS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.151"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(183..1502)
                     /locus_tag="IYA83_002638"
     CDS             complement(183..1502)
                     /locus_tag="IYA83_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ8588022.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DACIFS010000141         1457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.152, whole genome shotgun sequence.
ACCESSION   DACIFS010000141 DACIFS010000000
VERSION     DACIFS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.152"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            109..1041
                     /locus_tag="IYA83_002639"
     CDS             109..1041
                     /locus_tag="IYA83_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ8588023.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IYA83_002640"
     CDS             1113..>1457
                     /locus_tag="IYA83_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588024.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACIFS010000142         1441 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.153, whole genome shotgun sequence.
ACCESSION   DACIFS010000142 DACIFS010000000
VERSION     DACIFS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.153"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..257
                     /locus_tag="IYA83_002641"
     CDS             <1..257
                     /locus_tag="IYA83_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ8588025.1"
                     /translation="VRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHGKGLT
                     ANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            370..909
                     /locus_tag="IYA83_002642"
     CDS             370..909
                     /locus_tag="IYA83_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588026.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            1115..1297
                     /locus_tag="IYA83_002643"
     CDS             1115..1297
                     /locus_tag="IYA83_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ8588027.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
BASE COUNT          504 a          239 c          258 g          440 t
ORIGIN      
        1 aagtaagata tgaacgtgaa gctcaaaaag cgctaaaaaa aatggataag tttcaggcaa
       61 aggttatcct aaactggatc gagaaaaatc ttgtaggcac tgatgatcca agaaagcatg
      121 gtaaaggtct aacagctaat aaatcaggct actggcgata tagggtaggt gcatatcgta
      181 taatagctga tattagcgag catgaagtga caattcttat attgtctatt ggtcacagaa
      241 gtgaagttta taagtaacag acaagctgat aaggcttgtc tgtttttttg ttaacccaac
      301 aaaatcactg tttacgatcc cactttacct aaaaaagaac aatatcctct tgatattata
      361 cacaccggtg tgtataatat aagtgtaaac aagctaatta actttcaaaa ggagcgattt
      421 tttatgggat ggtatggtga ttattcatca gcaaatgaag tgatcgaaga aaagaaaggg
      481 tacgccaaaa atggtttgga aatcgtagat accaaagtaa cgaagatcta tggaattatc
      541 cttttcaaag atgcagaagg aacctacgat attgacagct tcatctttcg agatgaaatg
      601 tacaaaccaa tctacttctt aaatgacagt cctgcttact ttaagaaaat tccgaaaaaa
      661 tggttgaaat tctttgtcgg tgatcaagaa aaaatggtta agcgacaact agatagaatt
      721 aaagaagaaa gcaataaacc aaaacttgat gacttattag ttgcaggtaa aacttactca
      781 atctgggatg gatcgtatgt cgctgtctat cagtataaac tcaaacgtag ctttattttt
      841 cgactacaag atggatcact cactcggttc actcgactat cagcaggaga tgttaaggaa
      901 gtagcctaaa acttaaatac ataaacataa acgtatacac gtttatgttt atgtatatgt
      961 atatgaatat aaacgtatta ggtacgaacg cttttaaatt cttttctggg ctttgttttt
     1021 agggtagggc taaaaacatc accatctgat ccaaaacagc tcagaaacgc atttaaacgt
     1081 atctgatacc aatattacag aaggagtgta tcgtatgatt aacctatttc ttttagtgac
     1141 agctagtaca ttattcttta ctggaatatc actgataatt tcctatttga tctataagaa
     1201 agccgaaaat cacacaagca aagcaaccgg actagccgtt tcatcctttt taactttgct
     1261 gactttattt gttactttat cgttttcttt tgtttagaac agctgatcca ttagcacctt
     1321 tttctcttta cattatacac accgatgtgt ataatgtatt tgtaaacaag ctaattaact
     1381 tttaggaagg agtgacaaga tgctaacagc tactttacag caacctactc acgttgctat
     1441 t
//
LOCUS       DACIFS010000143         1437 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.154, whole genome shotgun sequence.
ACCESSION   DACIFS010000143 DACIFS010000000
VERSION     DACIFS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.154"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(163..1302)
                     /locus_tag="IYA83_002644"
     CDS             complement(163..1302)
                     /locus_tag="IYA83_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ8588028.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          373 a          304 c          268 g          492 t
ORIGIN      
        1 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
       61 ctctccttgt atgctttagc gggtattaca attggagtgt aacataaatg gcttttttat
      121 ttgtctcgct taattataca aacggcgttt attaaaaaaa tcttacgcta agtaggataa
      181 aataattcct tgatattttt cgatcatatc caaataatta tcgatttcaa catactcatc
      241 tacttgatga ggcaaagtaa cgacaccagg cccaaagacg acaaagtcga aggaatggtt
      301 agctttcgta aattcggctg catccgttgt tgctgccgct ccaactaaag gtaatggctg
      361 gctgaattgt tgctgaatgc aatgaatcaa aggagaatct ggatcagctt tgacaggtat
      421 tttgttgtaa tcgatcatca attccaaatg atagtctgtc tcttgattca attcattgac
      481 gattgattgc agcaatgcaa tgattttatc atttgggtat tctggaatcg atcgaatgtt
      541 cccttggagc tgcgcatggc ttgggatgct gttgacttga ttcccgccac tgatcaatgt
      601 aacattatgg attgtttttc ctaggacagg attctcgatt gtttcagcga ggtgattcat
      661 ttcagcattt gctttcgtaa tgaattcatt taggtgattg attgcattat aaccttggtc
      721 tggcatagag ctatgcgctt cttttccgtg cgaggtgact gtatagttga ttgatcccat
      781 gtgcgtatac atcaaactgt aattggttgg ctctccgatg atcaaggcat ctaagtcatc
      841 tacatagcct gctttcgtga gctgttcccc acctagttca cccacttctt ctccgacagt
      901 ggctaataat ttgactgttc cgttaaatgg ttttcctgac tctttcagtt cgatcatggc
      961 aatcaccatt gctgccaagc cactcttcat atctgtagca ccacgtccgt acaaacggtt
     1021 cccatggatt tctgctgcaa agggtgcata ggtccaagac gattcatcac cagccgccac
     1081 gacatccata tgcccagata ggcctaacac ttttcctgat tggccctttt ggtaacgagc
     1141 aatcaaatta tctctaccat ctctgtaaga aacaatctct cctgttatgt catgctttgc
     1201 caaaagctta ttcaaatagg ctgctacttc accttcgttt ccattcactg atttaattcg
     1261 gataatctct tgtaaaatcg cgattttttc ttcttttttc atctaatcct ctcccttgcc
     1321 atttcttttt attgtatttg agtgtgctcg aattttcaaa taggagcaac caaagtaaaa
     1381 atgaagaaag agagccactt tatgaaaaat atgctgctac ttccctccta ttttatt
//
LOCUS       DACIFS010000144         1428 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.155, whole genome shotgun sequence.
ACCESSION   DACIFS010000144 DACIFS010000000
VERSION     DACIFS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.155"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..525
                     /locus_tag="IYA83_002645"
     CDS             <1..525
                     /locus_tag="IYA83_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAQ8588029.1"
                     /translation="EYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLR
                     NIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGF
                     EDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHD
                     EWIYSSRKYINFDK"
     gene            691..888
                     /locus_tag="IYA83_002646"
     CDS             691..888
                     /locus_tag="IYA83_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ8588030.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            890..1201
                     /locus_tag="IYA83_002647"
     CDS             890..1201
                     /locus_tag="IYA83_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ8588031.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            1305..>1428
                     /locus_tag="IYA83_002648"
     CDS             1305..>1428
                     /locus_tag="IYA83_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ8588032.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
BASE COUNT          546 a          191 c          255 g          436 t
ORIGIN      
        1 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
       61 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
      121 cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc tttcagagaa
      181 gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc taaaaatcga
      241 ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc attggatgat
      301 ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg actaaagagt
      361 accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat tattcgcatc
      421 ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga cctgcatgat
      481 gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg gtaaaaacat
      541 tgtatagcat tttacacagg agtctggact tgactgatta aattcttatt caggaattat
      601 tttgctacgt ttcaagaagg aattgttttt tgaaatttaa tgaatgttag aataatagtc
      661 taaaaacatt aatttagggg tgattagatt atgaaacatt taaaaatttt gtctattaaa
      721 gagacacaac ttatctatgg gggtaccact catagtggaa aatattatgg aaatggagtg
      781 tattgcacta aaaataaatg tacggtcgat tgggccaagg caactacttg tattgcagga
      841 atgtctatag gtggtttttt aggtggagca attccaggga agtgctaaaa tgaaaaaaaa
      901 tgctaagcaa attgttcatg aattatataa tgatatatct ataagtaaag atcctaaata
      961 ttctgatatt cttgaggttt tacaaaaggt atatttaaaa ttagaaaaac aaaaatatga
     1021 attagatccc agtcctttaa taaatagatt ggtgaattat ctatatttta ctgcttatac
     1081 taataaaata agattcactg aatatcaaga ggaattaata agaaatttga gtgaaattgg
     1141 aagaactgct ggaataaatg gtttatatcg agcagattat ggagataaat ctcaatttta
     1201 atgatactta taatattaaa tatgtctcaa aattttttga ttagaaaaat aggtatattg
     1261 aatcagtgac ataaaagtat ttatagtttg tagggggaat cgtaatggaa gaaaaaaata
     1321 gactaaatgc taaacaatgt tcagatcaag agcttaaaaa aattaaagga ggtgctggaa
     1381 caaaacctca aggaaaaccg gcatcaaatt tagtggaatg tgtttttt
//
LOCUS       DACIFS010000145         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.156, whole genome shotgun sequence.
ACCESSION   DACIFS010000145 DACIFS010000000
VERSION     DACIFS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.156"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(150..1337)
                     /locus_tag="IYA83_002649"
     CDS             complement(150..1337)
                     /locus_tag="IYA83_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ8588033.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACIFS010000146         1423 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.157, whole genome shotgun sequence.
ACCESSION   DACIFS010000146 DACIFS010000000
VERSION     DACIFS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.157"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            98..1390
                     /locus_tag="IYA83_002650"
     CDS             98..1390
                     /locus_tag="IYA83_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ8588034.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          257 g          411 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tccacttgat ctc
//
LOCUS       DACIFS010000147         1394 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.158, whole genome shotgun sequence.
ACCESSION   DACIFS010000147 DACIFS010000000
VERSION     DACIFS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.158"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>1394
                     /locus_tag="IYA83_002651"
     rRNA            <1..>1394
                     /locus_tag="IYA83_002651"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          296 t
ORIGIN      
        1 taagtctgaa gaggagtcaa atgcttcttt tcttaaggac aagctgtgat ggggagggaa
       61 ataatagtac cgaagttcct gatgtcacac tgccgagaaa agcttctagt gagaaaacag
      121 cggcccgtac cgcaaaccga cacaggtagt cgaggagaga atcctaaggt gagcgagaga
      181 actctcgtta aggaactcgg caaaatgacc ccgtaacttc gggagaaggg gtgctgatca
      241 tacgatcagc cgcagtgaat aggcccaagc gactgtttat caaaaacaca ggtctctgca
      301 aaatcgtaag atgaagtata ggggctgacg cctgcccggt gctggaaggt taagaggagt
      361 gcttagcgca agcgaaggta cgaattgaag ccccagtaaa cggcggccgt aactataacg
      421 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga
      481 tttgggcact gtctcaacga gagactcggt gaaattttag tacctgtgaa gatgcaggtt
      541 acccgcgaca ggacggaaag accccatgga gctttactgt agtttgatat tgagtgtctg
      601 taccgcatgt acaggatagg taggagccgt agaaatcgga acgctagttt cgatggaggc
      661 gctggtggga tactacccct gcgttatggc cactctaacc cgcaccacta atcgtggtgg
      721 gagacagtgt cagatgggca gtttgactgg ggcggtcgcc tcctaaaagg taacggaggc
      781 gcccaaaggt tccctcagaa tggttggaaa tcattcgaag agtgtaaagg cagaagggag
      841 cttgactgcg agaccaacaa gtcgagcagg gacgaaagtc gggcttagtg atccggtggt
      901 tccgcatgga agggccatcg ctcaacggat aaaagctacc ctggggataa caggcttatc
      961 tcccccaaga gtccacatcg acggggaggt ttggcacctc gatgtcggct cgtcgcatcc
     1021 tggggctgta gtcggtccca agggttgggc tgttcgccca ttaaagcggc acgcgagctg
     1081 ggttcagaac gtcgtgagac agttcggtcc ctatccgtcg cgggcgttgg aaatttgaga
     1141 ggagctgtcc ttagtacgag aggaccggga tggacttacc gctggtgtac cagttgttct
     1201 gccaagggca ttgctgggta gctatgtagg gaagggataa acgctgaaag catctaagtg
     1261 tgaagcccac ctcaagatga gatttcccat ttctttaaga aagtaagatc cctgagagat
     1321 gatcaggtag ataggtcagg agtggaagta cagtgatgta tggagcggac tgatactaat
     1381 cgatcgagga ctta
//
LOCUS       DACIFS010000148         1368 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.159, whole genome shotgun sequence.
ACCESSION   DACIFS010000148 DACIFS010000000
VERSION     DACIFS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.159"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            82..>1368
                     /locus_tag="IYA83_002652"
     CDS             82..>1368
                     /locus_tag="IYA83_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ8588035.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DACIFS010000149         1355 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.160, whole genome shotgun sequence.
ACCESSION   DACIFS010000149 DACIFS010000000
VERSION     DACIFS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.160"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..216
                     /locus_tag="IYA83_002653"
     CDS             <1..216
                     /locus_tag="IYA83_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ8588036.1"
                     /translation="YKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYET
                     PIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            323..538
                     /locus_tag="IYA83_002654"
                     /pseudo
     CDS             323..538
                     /locus_tag="IYA83_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(829..1353)
                     /locus_tag="IYA83_002655"
     CDS             complement(829..1353)
                     /locus_tag="IYA83_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588037.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          461 a          217 c          198 g          479 t
ORIGIN      
        1 tataaatctg tcaaagtaga attcgtgcat caataccaat ttgagacact ggcacagcta
       61 cgtctagaat tgtttgatta tgtgcattgg tggaactatc tacgcttaca tggcacgttg
      121 gcgtatgaaa caccgatcca aattcgacaa cagagattgg cgaagcgaat ccttgataat
      181 gagcgcggat ctgatacctc tggagaggca gcgtaattgg aatgattgtg cttctgccgg
      241 agaaaatcag atccgaggat gctcattgtc aagggcaatc ggagccataa caccgcagca
      301 ttcacaacac cttataattt ttgtcaaaaa aagtgttgcc attccaacca atctaatcga
      361 gtctttcaat aagcaaatta aaagatacag ccgtagaaaa gagcagtttc aaaatgaaga
      421 atcactagaa cgctttctag tcagcatttt tgatacatac aatcaaaaat ttctaaacag
      481 aagccataaa ggttttcaac aggtaaccga tacattagtt tcaatgttta ctgagtaact
      541 aattattttg caggaggaca atttatttac acaaaattat tgacgctccc ttaacaataa
      601 atgaagactt ttttattgtt aattgtttat ttttctatta ttagatatga aaatcgcatt
      661 tagataaaat agaaaaatat gaatatattt taaatattaa gatgagagtc attaacttat
      721 gatagtagtc aatttgcttt tcactaatta caaaatatgt gatataggaa gtttagttat
      781 tgttctcata atgccatcct gtttttagat tatctagtaa tttttttgtt atttaaaatt
      841 aggagcagtg ccaattgttt taattttaaa ggcttttttt atatcttcta aattagaaga
      901 tatttgtata tcaatatgga atatattacc ttttttgtat ttttttatgt tatctttagc
      961 taaaacatca aatacatgtc cttgaaattg tatcgaggca gctggattta gtttaaattg
     1021 aggaaacttt accacagctg attcaaaaca cttgtcacat ttttttattt ttttaccttt
     1081 gtaaaaatat tcgattgaat attctatttg gcacgactca cttggcagta tatgtatgtc
     1141 aatatttttc atctgtttca taggtatttc aacatctaaa ctattaaaaa catataattc
     1201 cctgtaatta tttacatcta actctactaa atttatcttt ggaacaatag atcctccacc
     1261 cattccacca taataaaaaa aatcttgttc tctatcaaaa tagcacatat taagctcagt
     1321 ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACIFS010000150         1352 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.161, whole genome shotgun sequence.
ACCESSION   DACIFS010000150 DACIFS010000000
VERSION     DACIFS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.161"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            11..1350
                     /locus_tag="IYA83_002656"
     CDS             join(11..452,452..1350)
                     /locus_tag="IYA83_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ8588038.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          565 a          175 c          235 g          377 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaag ttagaaacct tgattcaaga
      481 acaagatttg agcaagaaga aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt
      541 aaattgaatc tattactttc aattgcacaa ttagcgaagt caacttatta ttactgggta
      601 aaaaaattag ataagccaga taaatatagc aagataaagc aagaaattac agcgattgta
      661 aaagaatcaa gaaactctta tggttatcgt agagtcactt tagcgttaaa gatgaaagga
      721 tatacaatca atcataaaac agttagaaaa ttaatgtccc aaatgggact tacctgtcaa
      781 atccgaataa agcgatataa atcttataaa ggaacagtag gaaaaattgc caagaatgtg
      841 ttaaaacgaa atttttcagt agatacaccc aataaaaaat gggtgacaga cgtcactgaa
      901 ttcaagataa aaggaagaaa aatctatcta tctcctatac ttgatttatt taacggagaa
      961 ataattagtt atagtatatc aactagtcca acatataaac tgattgaaga aatgcttcaa
     1021 caagcaatta aaaaaaaggg aactgagggt tcactgattc tacattcaga tcaaggatgg
     1081 cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg
     1141 tctagaaaag gaaattgctt agataattct gtaatagaaa atttttttgg tgtgcttaaa
     1201 tcagagtttt tttatcgaga aaaattccga tcaattgaga tatttcaaag taaattaaat
     1261 gagtatatta ggtggtataa taacaaaagg ataaaactaa agttaaatgg actatctcca
     1321 gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACIFS010000151         1276 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.162, whole genome shotgun sequence.
ACCESSION   DACIFS010000151 DACIFS010000000
VERSION     DACIFS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.162"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            35..532
                     /locus_tag="IYA83_002657"
     CDS             35..532
                     /locus_tag="IYA83_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ8588039.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IYA83_002658"
     CDS             complement(569..1252)
                     /locus_tag="IYA83_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588040.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACIFS010000152         1257 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.163, whole genome shotgun sequence.
ACCESSION   DACIFS010000152 DACIFS010000000
VERSION     DACIFS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.163"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            335..868
                     /locus_tag="IYA83_002659"
     CDS             335..868
                     /locus_tag="IYA83_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588041.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            880..1083
                     /locus_tag="IYA83_002660"
     CDS             880..1083
                     /locus_tag="IYA83_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588042.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT          435 a          178 c          253 g          391 t
ORIGIN      
        1 aacccagtaa aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc aaccgcagaa
       61 aatcaagctt aaaaagagct tttgacctag accaatcgaa aatcacaaag tgagcctctg
      121 tatcggttga tacaagggct acttggcata gtcgacatta tgtcaacaag aatttaatat
      181 aacatatttt cctaatgcat tctaactata ccacattatc aaaaatataa attatcttta
      241 ctaaataaaa tattgacaag cctatacctt ttgttttatg attttcacaa agaaaacgtt
      301 ttctatatat ttttctgtat caggaggaaa ttctatgaaa aaatttactt taggggtagc
      361 tttgttaact attggggcag ttgttttacc gtcaactact atagtttatg cttcagaaaa
      421 ttcccaagtg gtatcattaa acgaaaacaa tgctactgaa aatgtagtta ttgagtacac
      481 tgaagatggt atggtcgtaa ctaaatatga ggatggttat agtgcggaag attttgcaaa
      541 ttcttcagat atcatgacaa gagctgcagt tgcaagatgg ggcaaaaagg aatatacaaa
      601 tattgctgta accactggag tagctgctaa tgctattaat gctgcctttt gggcaggagg
      661 gaaagctagc acttctatgt ttggtatccc aggttgggca attggtgctt tgttaacagg
      721 tgcaagttgg actaaaaaag gtgcttctcc agggaatgca gttgcaaaag aatgggacaa
      781 aaatggtaat ggctggattg gattttatta ccaaagggga tatgatgcag ctggaagagt
      841 tgtagctact agatattcaa cgttataagt taaggagtga tgtttttgat atctaaatca
      901 ttaaaatcat taaaaacatt tactaaaaag aatccgcaaa tagctactat tcgtattttt
      961 atatttgcta tagctgttgc tataattgtt aatatgctaa gcggagggca attatacaaa
     1021 tctggtttat ttttagtgtt aatcttagtt gtcttagaca taataaagtc taaaaaaaaa
     1081 taaatcgata cattttagaa gaatagggtt gtttgaatat tacaaagcaa caatttcacc
     1141 ttcggtgaaa taagcgaaac tttacaaaaa gtggctagca aatgctagcc tttttttgta
     1201 ttgtggagcg tggattttga gggggattca agggggagct tgcgctcctt tgacaag
//
LOCUS       DACIFS010000153         1218 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.164, whole genome shotgun sequence.
ACCESSION   DACIFS010000153 DACIFS010000000
VERSION     DACIFS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.164"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            1..999
                     /locus_tag="IYA83_002661"
                     /pseudo
     CDS             1..999
                     /locus_tag="IYA83_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          430 a          218 c          248 g          322 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg agacaccggt tggttaccgt aaccagagat tggcgcagcg aatccttgat
      961 aatgagctcg gatgtgctaa cgctagcgag gcagtctaac tttaactgtt agctcctgcc
     1021 gaagatcgtc acatccgagg aggctcattg tcaaggacaa tcggaatagc atacggaaag
     1081 aagttctgca ccttataaaa tttgtcaaaa aaactgttgc cattccaatg agaaaaagca
     1141 tggaatgcgc tgcatcaggt atcgaggctt agaaaaaaat caggtgcata cgatgcttgt
     1201 ttttgccgaa atgagtct
//
LOCUS       DACIFS010000154         1195 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.165, whole genome shotgun sequence.
ACCESSION   DACIFS010000154 DACIFS010000000
VERSION     DACIFS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.165"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..1076
                     /locus_tag="IYA83_002662"
     CDS             <1..1076
                     /locus_tag="IYA83_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ8588043.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACIFS010000155         1189 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.166, whole genome shotgun sequence.
ACCESSION   DACIFS010000155 DACIFS010000000
VERSION     DACIFS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.166"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            142..399
                     /locus_tag="IYA83_002663"
     CDS             142..399
                     /locus_tag="IYA83_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588044.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(631..1044)
                     /locus_tag="IYA83_002664"
     CDS             complement(631..1044)
                     /locus_tag="IYA83_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ8588045.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          437 t
ORIGIN      
        1 tttctgtcca cgttcatttt actccaaagt tttcctcatt tcaaaagaaa atcacagaat
       61 acacgaagaa gcgtttctat ttgtataaac tatatcaaag ttctatactt aaattatcaa
      121 ataaaaagga cgtgaagata aatgtcaaat ttagaaaaca aagaagaaaa agttgtaaac
      181 aaaattgttt ctgttgtcaa taaactggat aaagagttag acgaacttga tacgttatct
      241 gaaaatccag aaaaaaaaca taacctaaaa aaatggttag ttgaacgaaa ggctattcat
      301 gaaattaaaa aagttcttca tgaagctgat aaatatgaaa aatatgatga aaaagaactt
      361 gataaagaat ttaaagaaat caatgactta ttactataat tgatttatac gactcatttt
      421 tgagtcgtca gactgcaaac aacactctaa atttaggagt ttgtctacag tctgaataaa
      481 ttttaaattt attgtccata atgttattat gaatttatag cattaagaaa agagataaga
      541 ctttacattt ttttaggaat gtaaagtctt atctcttttc ttaattgtac aattagtatt
      601 atctgcacta ctttctaatt aaataaattt ttagttatta acttgattaa aatcagagat
      661 aatatcattt aattcagttt gaagggttgt aacgattaaa tggaaatttt cttcatctgt
      721 tttattatat ggggatgttt tcgttatata taacaacttt tctttttcaa ttttcattaa
      781 taactctgtc gaagtctgta ctttatcttg ataattaaat gcagacatac cactttgtat
      841 aattgtaatt attgctgaag caattgaaac aatcaatagt aaagacctgt gttcggaagc
      901 ctgatcaatt aatattggga tactagctga taagactatt tttataatat ttccgatacc
      961 aattactcta ttataaaatc gaatttcttt ttttaatcta tcgattgtag aatcaataga
     1021 ttcgataaac gctttttgat ccatcatttt ctctccaatc attttattaa aatcattaac
     1081 aaaaatatta taataggatt taagtaagtt ctaactaatc acatataaaa ataaaaaaac
     1141 atttgtattt tatcaaagtt ttattatact tgtcaatttg tgtacatcc
//
LOCUS       DACIFS010000156         1102 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.167, whole genome shotgun sequence.
ACCESSION   DACIFS010000156 DACIFS010000000
VERSION     DACIFS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.167"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(78..278)
                     /locus_tag="IYA83_002665"
     CDS             complement(78..278)
                     /locus_tag="IYA83_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588046.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            complement(363..734)
                     /locus_tag="IYA83_002666"
     CDS             complement(363..734)
                     /locus_tag="IYA83_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588047.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
BASE COUNT          379 a          183 c          111 g          429 t
ORIGIN      
        1 tctttattag aaatttcact cataacacga taatatagat catgattcaa gtatcggaat
       61 ttcgacctca attgctttta gaattgtacc gtatccagta aattcttacg ccagtccatc
      121 actaattttt caaattcatc ttcatcaaaa ctaaattcta atccttcttt tttaaacagt
      181 aactttctat ctatacatct cgagaaataa ttgtatttat attctttaag ctcaatatct
      241 gttttattat ctttatccca tgcaattctt tttaacatat taccacttaa tccttcatgt
      301 aataactgct tttttatttg aataggtgta tacaagttat taaagtaaat tgctgaacct
      361 atttatatcg gaattaacag tccaagagag accttggcta ttaactcaga ttcaactatg
      421 aaaatataaa aaaaccaaat aaatgaaggg attaaccaag caaaaatttc taatatcaca
      481 ttaaatcttt tcatttttcc aaaactacta agacttgata ctaattcaat caaaagctta
      541 tcgctacttg ctgattcatc atatttagtt cctagattat ctaaacttag catataagaa
      601 attttttcct gatctgttag ttttgaaaat gaactttctt ttaaactttg gattatacaa
      661 tcagcactaa atatttcgtt taataaagca gttttctttt ctaattcctt agaagataaa
      721 gttagttctc tcatagtgac ttacctactt tccaaatttt gtgtcctatt tatcacttct
      781 ctctttatat aagtgataaa agatcgtaaa tacgtttaat tgttaagttc atattcacat
      841 gattatccaa ataaaatatc ttatctttat tagaaatttc actcataaca cgataatata
      901 gaccatgatt caagtaatct gtatatagaa aaatcgcatc acacgtattc aaatttttaa
      961 gtatctttcc attattttcg cttacatcaa acattttaat atttggtaat agttcagtta
     1021 atttattttg aaaatttaca tgtcctccca ctaaaagaat gtttttatgt tttagataag
     1081 ctatcatttc agacagtgaa gg
//
LOCUS       DACIFS010000157         1091 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.168, whole genome shotgun sequence.
ACCESSION   DACIFS010000157 DACIFS010000000
VERSION     DACIFS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.168"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            6..980
                     /locus_tag="IYA83_002667"
     CDS             6..980
                     /locus_tag="IYA83_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ8588048.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACIFS010000158         1085 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.169, whole genome shotgun sequence.
ACCESSION   DACIFS010000158 DACIFS010000000
VERSION     DACIFS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.169"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>1085
                     /locus_tag="IYA83_002668"
     rRNA            <1..>1085
                     /locus_tag="IYA83_002668"
                     /product="23S ribosomal RNA"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DACIFS010000159         1062 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.170, whole genome shotgun sequence.
ACCESSION   DACIFS010000159 DACIFS010000000
VERSION     DACIFS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.170"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            79..>1062
                     /locus_tag="IYA83_002669"
     CDS             79..>1062
                     /locus_tag="IYA83_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ8588049.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIY"
BASE COUNT          389 a          201 c          179 g          293 t
ORIGIN      
        1 aaaatccata caaaaaagga agtcccttct gtagaataaa gttaacgaca accaattcac
       61 agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac tctagtcact
      121 aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa cacactccta
      181 cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg ttttaattca
      241 ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca
      301 ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc agcctacaaa
      361 agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg tgttacgatg
      421 tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc acaaaccatg
      481 tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag agccttaaat
      541 gataagtatg tcgctatttt tatggacgct acttacattc cactaaaacg tcaaaccgta
      601 tccaaagaag cgatttatat tgccattggt atacgagaag acggcactaa agaagtactg
      661 agttatgcga ttgctccaac tgaatcaaca tacgtttgga atgagctgct acaggatatt
      721 aactccagag gagttcaaga agtcttgctt tttattacgg acggcttaaa aggcatgaaa
      781 gatactatcc atcaaattta tcctaaagca aaatatcagc attgttgtat ccatgtatct
      841 cgtaacatcg ctcataaagt acgtgtcaaa gaccgaaaag aaatctgtga tgactttaag
      901 gctgtttatc aagctaactc aaaagaagaa gcgaatacct tcttatccgg catgattgag
      961 aaatggaaga aaaactatcc taaagtgacg cagtcactca tagaaaacca agacttatta
     1021 actttttatg attttccacc tagcattcgt agaaccattt ac
//
LOCUS       DACIFS010000160         1060 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.171, whole genome shotgun sequence.
ACCESSION   DACIFS010000160 DACIFS010000000
VERSION     DACIFS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.171"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(38..994)
                     /locus_tag="IYA83_002670"
     CDS             complement(38..994)
                     /locus_tag="IYA83_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588050.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DACIFS010000161         1037 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.172, whole genome shotgun sequence.
ACCESSION   DACIFS010000161 DACIFS010000000
VERSION     DACIFS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.172"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..193
                     /locus_tag="IYA83_002671"
     CDS             <1..193
                     /locus_tag="IYA83_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ8588051.1"
                     /translation="TNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLN
                     RSHKGFQQVTDTLVSMFTE"
     gene            246..1028
                     /locus_tag="IYA83_002672"
                     /pseudo
     CDS             246..1028
                     /locus_tag="IYA83_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          436 a          151 c          137 g          313 t
ORIGIN      
        1 aaccaatcta atcgagtctt tcaataagca aattaaaaga tacagccgta gaaaagagca
       61 gtttcaaaat gaagaatcac tagaacgctt tctagtcagc atttttgata catacaatca
      121 aaaatttcta aacagaagcc ataaaggttt tcaacaggta accgatacat tagtttcaat
      181 gtttactgag taactaatta ttttgcagga ggacaattta tttacacaaa attattgacg
      241 ctccccaaga tatgctagat tactactatt ttgaaaaaaa tttatcatat aattatgtaa
      301 tacatttaaa aacttttaca ggaatgattt ttaaattcgc caaccgtcga tatggattaa
      361 aatataatcc cgtaaatggg gtttctttag caagaaaacc taagacaaac gaagaaataa
      421 aaaaagaaaa agatggttat ttatcaaaag aagaagttca aatgattgct agtaaacaaa
      481 aaagttcaca tcaacagtca cgttattctt taattactaa atttttgttt ttaactggac
      541 tacgttatgg tgaattgata tctttgactg aatctgatta tgatggtaat aaaattacgg
      601 tttctggtac ttatgattac gagttaaaaa ttaaaacaac aactaaaaat acaggttctt
      661 atagaaccat tgaattatca agtaatgcta aagaaatcat agatcaatta attgaagaaa
      721 acaaattaat taagaataat aaagataaat acattttcat ctctaaaaat ggtaacccga
      781 tatctattca agcatataat caatctttaa gagcagtatc caaagaatta aacatagata
      841 aaaaagtttc ttctcacatg ttaaggcatt cccacatttc tctacttaca gaattaggaa
      901 tcccattaaa agccattatg gatcgtgtag gacatgaaga ttcaaaaaca acattaaaga
      961 tttatactca tacaactaaa aatatgcaaa atcaactagt agaaaaacta ggaaaaatag
     1021 aaatttaaat tgcccct
//
LOCUS       DACIFS010000162         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.173, whole genome shotgun sequence.
ACCESSION   DACIFS010000162 DACIFS010000000
VERSION     DACIFS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.173"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..>1035)
                     /locus_tag="IYA83_002673"
     rRNA            complement(<1..>1035)
                     /locus_tag="IYA83_002673"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACIFS010000163         1000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.174, whole genome shotgun sequence.
ACCESSION   DACIFS010000163 DACIFS010000000
VERSION     DACIFS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.174"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            105..>1000
                     /locus_tag="IYA83_002674"
     CDS             105..>1000
                     /locus_tag="IYA83_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ8588052.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRA"
BASE COUNT          348 a          172 c          212 g          268 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc
//
LOCUS       DACIFS010000164          988 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.175, whole genome shotgun sequence.
ACCESSION   DACIFS010000164 DACIFS010000000
VERSION     DACIFS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.175"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            314..595
                     /locus_tag="IYA83_002675"
                     /pseudo
     CDS             314..595
                     /locus_tag="IYA83_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            669..>988
                     /locus_tag="IYA83_002676"
     CDS             669..>988
                     /locus_tag="IYA83_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ8588053.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVSH
                     ATIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRK"
BASE COUNT          358 a          129 c          180 g          321 t
ORIGIN      
        1 ctagactgtg caaaatagag acacttgaaa aatctatcgg gggtcagaaa cttaaaagtt
       61 tttgatcctt tattttcaag tctgatttgg cagcttttcg gcagctcatt tatttatggt
      121 cagaaataga tgctgaaatt ttaatctatt agcaaatagt ccagaacgta tgaagaggag
      181 tcggatatca tttgtcatta acaccataaa tatttataaa attcgtgttc acctacaatt
      241 aaatttaata tataatattc gttgtaaggg cttataattt tttgttaatg aacaagatag
      301 gaagaaacat ttaatggaca gttttcagag attgtcacta gtaaatcaat ttgagttatt
      361 ggctaaattt gaggaccaaa acagatctta ttatgaaaga aaaatcgaaa tattgagaga
      421 aggttacgag tatcattatg atgatgaaat ttggagtgat ctttctgagc catttccgaa
      481 agaagattct cgatttgtat tagatgtatt gaacatgtat cgagatatca attttagtaa
      541 atcaaagcta aacttagatg aaaaccaaat tactaattca tactatactc atttcggttc
      601 tgttgcaaag ttttccaaaa aatctatttt agtgtaaaat tgagaaaaaa gacagagagg
      661 gcagagtaat gaatcatttt aaaggcaaac aattcaaaaa agacgtcatt attgtcgctg
      721 ttggttacta cctgcgttac aatctaagct atcgtgaagt tcaggaattg ttatatgatc
      781 gtggcattaa cgtttctcat gcgacgattt atcgttgggt gcaagaatat ggcaaactac
      841 tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa atggatgaaa
      901 cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat gcagatggtt
      961 taaccttgga tatttggtta cgtaaaaa
//
LOCUS       DACIFS010000165          970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.176, whole genome shotgun sequence.
ACCESSION   DACIFS010000165 DACIFS010000000
VERSION     DACIFS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.176"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            82..>970
                     /locus_tag="IYA83_002677"
     CDS             82..>970
                     /locus_tag="IYA83_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ8588054.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANIN"
BASE COUNT          329 a          176 c          188 g          277 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc
//
LOCUS       DACIFS010000166          956 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.177, whole genome shotgun sequence.
ACCESSION   DACIFS010000166 DACIFS010000000
VERSION     DACIFS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.177"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..475
                     /locus_tag="IYA83_002678"
     CDS             <1..475
                     /locus_tag="IYA83_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAQ8588055.1"
                     /translation="RSEDNISISQYIATVIDELISGKYDYAFDFEGEKDYKSVAVFQS
                     DFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKA
                     ELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            512..679
                     /locus_tag="IYA83_002679"
     CDS             512..679
                     /locus_tag="IYA83_002679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588056.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            699..>956
                     /locus_tag="IYA83_002680"
     CDS             699..>956
                     /locus_tag="IYA83_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588057.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKV"
BASE COUNT          389 a          115 c          196 g          256 t
ORIGIN      
        1 tagaagtgaa gataatattt ctatctctca atatattgct acagttattg atgaattgat
       61 ttcaggaaag tatgactatg cttttgattt tgagggtgaa aaggattaca aaagtgttgc
      121 tgtctttcaa tctgattttg agaagctaaa aaactatgct ggcaggagaa atgttaattt
      181 tgcaagacta ctagaagagt tctactccga agagttggga gtaggaaatg aatacaagag
      241 agaaacttat gcgaaattgt ctcataagtt atcccaagcg cttaaagaag tgacagaagt
      301 taaagcggaa ctagatgcta aacttgaaga agtgaaaaaa tatcaaacag agaggaagaa
      361 caacatgaag aatcaagtgg aatttgacga acagaaagaa tttgaaaaag caagaaaacg
      421 ctataacaaa attatgaatg aacttagcta tgaacactta acagttcatg aataattgag
      481 tgagggtaca gaaaaatata cgatcgcaga tatggttaat gaagtggaaa ctcttttagc
      541 ttctttcttt gaaaatggtc acgtaaacga tgaatggcga aaagaagaac ctaaaagggt
      601 taacgcttat attgctcgat taaaaaggtt tattgataaa tataaggaga atagtgacaa
      661 attttctaaa gataaataag gagagagtgt gattacattt gccagaaatc gaaggaatat
      721 tttcgccaaa aataggtaaa aaacagttag atttcagcag ttttccgagc gtaaatagag
      781 attataatct aggtgatgag gtacagatac taaatattga tttcaaagaa ctagacttcg
      841 ctgttgctgt ttccaacaaa cccaaggcat tggaacgagt gaaaaaaata taccaagaga
      901 atgatcaaaa tatcaaaaat atgatcaaaa agtatagacg agataattca aaagtt
//
LOCUS       DACIFS010000167          889 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.180, whole genome shotgun sequence.
ACCESSION   DACIFS010000167 DACIFS010000000
VERSION     DACIFS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.180"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(12..>889)
                     /locus_tag="IYA83_002681"
     CDS             complement(12..>889)
                     /locus_tag="IYA83_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ8588058.1"
                     /translation="GSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFKQ
                     IAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTKD
                     YRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSPE
                     MKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRVK
                     LNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNAY
                     ELSLLYELLGPYIQK"
BASE COUNT          281 a          171 c          146 g          291 t
ORIGIN      
        1 ttttagacac tttatttttg aatatacggt cctaataatt catagagcag cgaaagctca
       61 taagcattga ctttaaaatt atatttcatc tccattgatt gaaaaatact tctagaaaca
      121 taataaaatt catcttcatc tggtttcaaa tcataattcg gttcttcaga tttttctgtc
      181 cgagccaaga ataaacgttc catcatcaat gccgtatgca tatagagatt tagttttaca
      241 cggccattta aagtgaaatg atagtaattt tcatatttag aaatgactgt ttccacttca
      301 ctcatcacga tcgttgggtt cagaaagttc aaacggtccg caattccttc caacgaaaag
      361 aattttacta gctcctgaat caagcggtta aattctgccg gcttcatttc aggactcaaa
      421 atatgctcca aattggctgc tccatcaccg tctaagatgt cataaacatt aatattagga
      481 attgaaaagg aacgtggaag atccgaagtc gtgatgacca attttgtctt gtcaaaataa
      541 gtttcatcat tttggtcgat taattcacgt agttctctgt agtccttcgt gaaaacttcg
      601 atggattcat ccgataaatg atgacgaaaa acatctttca atttgtccga aatccccaaa
      661 cccgacatac aggaaatgat aatgttgcta ctttgagaaa agccttcaaa gtattgaaca
      721 ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt taaatggcgc attcatcgtc
      781 atcttcaggc caatatcaag tgcaatggag gtcgtcaaat tattcagaag aagtaattct
      841 ccttttaaat cgttcttgat ctgtgtgtag atttgattta aagagccca
//
LOCUS       DACIFS010000168          846 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.181, whole genome shotgun sequence.
ACCESSION   DACIFS010000168 DACIFS010000000
VERSION     DACIFS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.181"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            305..>846
                     /locus_tag="IYA83_002682"
     CDS             305..>846
                     /locus_tag="IYA83_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAQ8588059.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLR"
BASE COUNT          315 a          111 c          157 g          263 t
ORIGIN      
        1 cattttcgac atgtacgaga tacagaattt acgagttatc atctttcagg aatagtaatt
       61 gacagtttcg tatatgaggc tatgggaaat tggaaattcg ttgaaaataa cagtgggggt
      121 caaaacatta gttctgtatc ttatgagaca gctttattag aatactataa ctcacataaa
      181 gttatgggcg gtttaaattt atactctcct ggaagcaatc aatttgttaa ttctgatagt
      241 agtatcatat gtttagaaaa agtattaaaa aaaatagctt tataaagttt tatggaggaa
      301 caacatggga gacgaaaaaa agcgcttgga agttcaatta cgtgaagcat atggacgagt
      361 aacgtatact tacaccactc atttaaagca agccgataat attatttctg acaataaaat
      421 tttaaggtgt cttcaaattg gtttgtcagc ccttactact ggaggggtac ttagtaccat
      481 tattactgat aatagtgagt taacctttgt tgctggatta tgttcaacaa tttctcttgg
      541 attaaactta tactttaaga attttgatct tgctgaagaa gctaaaaatc atcaaattgt
      601 tgccgataaa ttatggttga tcagagaaga atacgtaagc ttactaacag atttaaatcg
      661 tttaagtgtt gaagatattg tgaaaaaaag agatcaatta atgaacgaaa ctcatgaagc
      721 atataaaaat gcccctaaaa caaatagtaa aagctatgaa tcagctcaga aagcattaaa
      781 atctgaagaa gagcaatttt ttacagaaga ggaattggat aagatgttac cagagcatct
      841 tagaaa
//
LOCUS       DACIFS010000169          813 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.183, whole genome shotgun sequence.
ACCESSION   DACIFS010000169 DACIFS010000000
VERSION     DACIFS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.183"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            96..>813
                     /locus_tag="IYA83_002683"
     CDS             96..>813
                     /locus_tag="IYA83_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ8588060.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAP"
BASE COUNT          303 a          123 c          137 g          250 t
ORIGIN      
        1 tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt tgtttataac
       61 gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc gtgaaatacc
      121 atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg aatctatttt
      181 tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt acttttgacc
      241 aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt aatgatttag
      301 aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct aatgggctcg
      361 atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct aaagatccat
      421 atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta gatgagtggg
      481 ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag actatgtttc
      541 gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag gattttcatg
      601 aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat aatcgtgtta
      661 taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc aaaaagaaat
      721 acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa ccagaaatca
      781 aaaaagcaga tgattttaat aaacatgcac caa
//
LOCUS       DACIFS010000170          812 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.184, whole genome shotgun sequence.
ACCESSION   DACIFS010000170 DACIFS010000000
VERSION     DACIFS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.184"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..>812)
                     /locus_tag="IYA83_002684"
     CDS             complement(<1..>812)
                     /locus_tag="IYA83_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ8588061.1"
                     /translation="VGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKI
                     FEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATAN
                     NGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLL
                     DTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPF
                     SGNIGKLKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIAS"
BASE COUNT          245 a          176 c          137 g          254 t
ORIGIN      
        1 aaactagcaa tcggcaagct ttctacttcg gattccagca tatttaactg aatcaagatc
       61 tcatttttcc caatcgttaa tggttcttct tgtgttcgac tatacacatc cgcacaactg
      121 attttaatga tattcttcag cttaccaata ttcccactaa agggatgaga taacattaaa
      181 gacaaggctt ctcttgaaac aataatatct ttttgtaaaa catccgcttc attttgaaaa
      241 aataattgaa tcagctctaa tcgttctcga atggggcgat cttcaaaagt tggcaacata
      301 acactaattt gaatacgccg atcaaaggtg tctaataaat aattttcacc tttttccgtc
      361 gtggcgaaaa taaaacgaac cttagcagaa tgccaattta cattttcacc gactggacga
      421 tagtttccag tatcaataaa tagaaagagc ttttcttgat tttctttcga taggcgatgg
      481 acctcatcga gaaaaagata gccgttgttg gccgttgcca ataaaccatc ggtatccttt
      541 tcagcccccg taaaggcccc cttcttgtat ccaaacagtg ttgccgaaag cagttcggga
      601 ttgtccgcat aatcggcaca gtttaagata gcaaatggtg catctttttc aataatttgc
      661 tcatggatag cgtattcaaa aatttttgtg gctaaaaaac tttttccaac acccgtggct
      721 ccatttatta atacactgag tccgttaggc ggatatttga cagcggctat acatttttga
      781 atgacctctc tcagacttcc gtcatgacca ac
//
LOCUS       DACIFS010000171          811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.185, whole genome shotgun sequence.
ACCESSION   DACIFS010000171 DACIFS010000000
VERSION     DACIFS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.185"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IYA83_002685"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IYA83_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ8588062.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACIFS010000172          807 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.186, whole genome shotgun sequence.
ACCESSION   DACIFS010000172 DACIFS010000000
VERSION     DACIFS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.186"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>807
                     /locus_tag="IYA83_002686"
     CDS             <1..>807
                     /locus_tag="IYA83_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588063.1"
                     /translation="LASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAVGFIP
                     NTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERAKLLF
                     GNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAERLNNM
                     GDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQYIDHL
                     GRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNG"
BASE COUNT          260 a          116 c          186 g          245 t
ORIGIN      
        1 acttgcatca ggagcaggtg tgatggcaat ttcattaggt gttgttacaa catctctgat
       61 cggactacaa ttacctatct ttctgattac ttttattcgt gcatttggtg atactgctgt
      121 gggattcatt cctaatacat tagttggcga tggcgtagca aatattgttc gatctgctat
      181 gattttaggt gtaggatatc ttttagtctt taaaaaaggc gaaaaagtaa ataatttgat
      241 taatatgctt attggcttac cacttgaagt agcgtttggg tttgatgaac gtgctaagtt
      301 attatttggt aatagaaatg atgctgtgcg agttgtggac ggcgtttcta aagcaatcaa
      361 gaaaagccaa ggagctaaaa atccctatta tgatggtggc aacagctcag taaaagatat
      421 ggttgcagga ggtcttggag cagttggagc gcaaaaaatg gctgaacgtt tgaacaatat
      481 gggagatagc aatgctgatg ctaagtccat tgctgataaa gtaattcctg aaacaccaga
      541 aggaatgtca ttcaacggtg gcggagttgg ttccacaatc aataatacct ctttcaaaga
      601 tttagttcgt caagatcaac tacgtaataa ctcccaacaa tatattgatc atttaggacg
      661 tatggttgat gaaaacggaa actttgtcga tgaaaatggt gaaatattaa aagatagtaa
      721 atacggtaaa gatgctaaag gcaactttat tgacgaaaaa gggcgtttgg tcgatgaaga
      781 tggaaacctt atagatacta atggtaa
//
LOCUS       DACIFS010000173          797 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.189, whole genome shotgun sequence.
ACCESSION   DACIFS010000173 DACIFS010000000
VERSION     DACIFS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.189"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>797
                     /locus_tag="IYA83_002687"
     CDS             <1..>797
                     /locus_tag="IYA83_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ8588064.1"
                     /translation="RYKNIACKLKESICKQMTIDGPFGEQFLEGIGDEEKTTYSVKHY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDHLYQNTMAFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAFVTRLEELICLADLDGSWW
                     WWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGLKFKKGVLQIDPVDNLTFS
                     WKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI"
BASE COUNT          250 a          131 c          177 g          239 t
ORIGIN      
        1 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
       61 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
      121 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
      301 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
      421 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
      481 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
      721 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
      781 aaaagggaaa aacgatt
//
LOCUS       DACIFS010000174          797 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.190, whole genome shotgun sequence.
ACCESSION   DACIFS010000174 DACIFS010000000
VERSION     DACIFS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.190"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>797
                     /locus_tag="IYA83_002688"
     CDS             <1..>797
                     /locus_tag="IYA83_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ8588065.1"
                     /translation="RYKDIACKLKESICKQMTIDGTFGEQFLEGIGDEEKTTYSVKNY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDPLYQNTMTFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEELICLADLDGSWW
                     WWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVLQIDPVDNLTFS
                     WKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTI"
BASE COUNT          253 a          126 c          180 g          238 t
ORIGIN      
        1 agcgatacaa agacatcgcc tgtaaactaa aggaatcaat ttgtaaacaa atgaccatag
       61 atggtacttt tggggagcaa tttttggagg ggatcggtga tgaggagaaa acaacctatt
      121 cagtgaagaa ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg gaaaatcaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcctttata tcagaacacc atgacctttt
      301 ctgctagttc tttcaatcca acttatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagtgacatg cacaaccatc
      421 aagccttcgt aacccgatta gaggagctga tttgtttggc agatttagac ggttcatggt
      481 ggtggtggcc gtatcgttta gaggctaaac aaggagaagt tgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggcctttttg tcagtataat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 aaaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
      721 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
      781 aaaagggaaa aacgatt
//
LOCUS       DACIFS010000175          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.191, whole genome shotgun sequence.
ACCESSION   DACIFS010000175 DACIFS010000000
VERSION     DACIFS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.191"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            24..716
                     /locus_tag="IYA83_002689"
     CDS             24..716
                     /locus_tag="IYA83_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ8588066.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACIFS010000176          760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.192, whole genome shotgun sequence.
ACCESSION   DACIFS010000176 DACIFS010000000
VERSION     DACIFS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.192"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(30..102)
                     /locus_tag="IYA83_002690"
     tRNA            complement(30..102)
                     /locus_tag="IYA83_002690"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:His,seq:gtg)
     gene            complement(105..178)
                     /locus_tag="IYA83_002691"
     tRNA            complement(105..178)
                     /locus_tag="IYA83_002691"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Trp,seq:cca)
     gene            complement(185..265)
                     /locus_tag="IYA83_002692"
     tRNA            complement(185..265)
                     /locus_tag="IYA83_002692"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Tyr,seq:gta)
     gene            complement(267..342)
                     /locus_tag="IYA83_002693"
     tRNA            complement(267..342)
                     /locus_tag="IYA83_002693"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Phe,seq:gaa)
     gene            complement(378..450)
                     /locus_tag="IYA83_002694"
     tRNA            complement(378..450)
                     /locus_tag="IYA83_002694"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Asp,seq:gtc)
     gene            complement(460..532)
                     /locus_tag="IYA83_002695"
     tRNA            complement(460..532)
                     /locus_tag="IYA83_002695"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(497..499),aa:Val,seq:tac)
     gene            complement(548..619)
                     /locus_tag="IYA83_002696"
     tRNA            complement(548..619)
                     /locus_tag="IYA83_002696"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(584..586),aa:Glu,seq:ttc)
     gene            complement(634..723)
                     /locus_tag="IYA83_002697"
     tRNA            complement(634..723)
                     /locus_tag="IYA83_002697"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(687..689),aa:Ser,seq:gga)
BASE COUNT          209 a          209 c          176 g          166 t
ORIGIN      
        1 ccgcttggct ataccccaag aataaaaagg gcgaatgatg ggaatcgaac ccacgaatgc
       61 cggagccaca atccggtgcg ttaaccactt cgccacattc gccatggcag gggcagcagg
      121 aattgaaccc acactaacgg ttttggagac cgctgttcta cctttaaact atgcccctat
      181 aaaatggagg agagtggatt cgaaccactg aaccctaagg aacggattta cagtccgtcg
      241 cgtttagcca cttcgctact cctccatggt ggcgcgggac agaatcgaac tgccgacaca
      301 cggagcttca atccgttgct ctaccaactg agctaccgcg ccatatttta aaattattaa
      361 ttgaataaca agaacgacgg ttccgacggg aatcgaaccc gcgatctcct gcgtgacagg
      421 caggcatgtt aacccctaca ccacggaacc agttcattat ggaggttaac gggatcgaac
      481 cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaacct ccaacattca
      541 attaaaatga cccgtacggg attcgaaccc gtgttaccgc cgtgaaaggg cggtgtctta
      601 accgcttgac caacgggcca ggtaaatttg ctacggagag taagggattc gaacccttga
      661 gacagtttgg caccatctac atgatttcca atcatgctcc ttcggcctct cggacaactc
      721 tccagagtga aagaagctca atactctttc tcaaaatgag
//
LOCUS       DACIFS010000177          759 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.193, whole genome shotgun sequence.
ACCESSION   DACIFS010000177 DACIFS010000000
VERSION     DACIFS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.193"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..362
                     /locus_tag="IYA83_002698"
     CDS             <1..362
                     /locus_tag="IYA83_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588067.1"
                     /translation="LSVILSLKIFNQINTSSNAKDGIVVENTSNSKETGTTTKKDKSA
                     EITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDKFNGTFSLQTAEGSPDNLKV
                     GQTIILGKNDELYSFVK"
     gene            complement(379..>759)
                     /locus_tag="IYA83_002699"
     CDS             complement(379..>759)
                     /locus_tag="IYA83_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588068.1"
                     /translation="RQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVREELR
                     LNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMYLQH
                     PQYLNFGVEIATPLEEWKMEQGLD"
BASE COUNT          255 a          146 c          116 g          242 t
ORIGIN      
        1 ttcttagcgt tatcttgtcg ctgaaaatat tcaatcaaat aaacacaagc tcaaatgcca
       61 aagatggaat agtcgtagaa aatacctcta attctaaaga aactggtact actacaaaaa
      121 aagacaaatc tgctgagatc actacgaaaa attatttaat aaagactgtt tattttgatg
      181 aacgaaaaac agcaattctc aaagatgagg aaggatcaga attaaagcta cctataacgg
      241 tatccgataa gttcaatgga acgttctcct tacaaaccgc tgaaggatca cctgacaatc
      301 tcaaagttgg gcaaacaatc attcttggta aaaacgacga actgtattca tttgtaaaat
      361 aaaaaaatgg agcaaaggct agtcaagccc ttgctccatt ttccattctt caagaggtgt
      421 agctatttca acaccaaagt ttaaatactg tggatgctgt agatacatga tatccattct
      481 attttcgata aactctttta acgttgagta gatgtatttt tctaatgcta tatctgccca
      541 actaccataa tcaagtttat tcgtgatctt gacgtttatt tcaatttgag aaacatcaat
      601 attttgaaca tcaggaaact tattcagtct tagctcttct ctaaccgctt ctactagcgg
      661 ttcatattgt ccgcctagtt cgctaattgt tgaaactatt tcatctgaat tccaacgatc
      721 ttccaatacc tttgctacat tttctttagg cggttgtct
//
LOCUS       DACIFS010000178          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.195, whole genome shotgun sequence.
ACCESSION   DACIFS010000178 DACIFS010000000
VERSION     DACIFS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.195"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            23..>731
                     /locus_tag="IYA83_002700"
     CDS             23..>731
                     /locus_tag="IYA83_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ8588069.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          257 a          126 c          139 g          209 t
ORIGIN      
        1 gaattataag gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat
       61 aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg
      121 ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga
      181 atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc
      241 cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat
      301 tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa
      361 aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca
      421 tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta
      481 tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca
      541 taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc
      601 gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc
      661 gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca
      721 gatcagtaat g
//
LOCUS       DACIFS010000179          731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.196, whole genome shotgun sequence.
ACCESSION   DACIFS010000179 DACIFS010000000
VERSION     DACIFS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.196"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            119..448
                     /locus_tag="IYA83_002701"
     CDS             119..448
                     /locus_tag="IYA83_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8588070.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..725
                     /locus_tag="IYA83_002702"
     CDS             438..725
                     /locus_tag="IYA83_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ8588071.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKIIPVLLCGTHEKFYEQLKKLI"
BASE COUNT          283 a           80 c          138 g          230 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggtttaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaaga
      661 ttattccagt attgttatgt ggaacgcatg aaaaatttta tgaacagttg aaaaaattaa
      721 tctagtgatt t
//
LOCUS       DACIFS010000180          729 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.197, whole genome shotgun sequence.
ACCESSION   DACIFS010000180 DACIFS010000000
VERSION     DACIFS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.197"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..703
                     /locus_tag="IYA83_002703"
     CDS             <1..703
                     /locus_tag="IYA83_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ8588072.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIMIRHGKTFLTK"
BASE COUNT          235 a          147 c          136 g          211 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt
      721 tttcttctt
//
LOCUS       DACIFS010000181          728 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.198, whole genome shotgun sequence.
ACCESSION   DACIFS010000181 DACIFS010000000
VERSION     DACIFS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.198"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..>728)
                     /locus_tag="IYA83_002704"
     CDS             complement(<1..>728)
                     /locus_tag="IYA83_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ8588073.1"
                     /translation="AVESPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTS
                     AKITIENLHPKDMSSFFYTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLK
                     GQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDE
                     FGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDP
                     ICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          218 a          173 c          139 g          198 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttcgaa ataggaatta aagccccccg taggattaac cactataggc
      661 aacgagttca cctcgcaacg ctctccaaac ccattacaaa agaaatcccc taaaggactt
      721 tcaacagc
//
LOCUS       DACIFS010000182          726 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.200, whole genome shotgun sequence.
ACCESSION   DACIFS010000182 DACIFS010000000
VERSION     DACIFS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.200"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            61..414
                     /locus_tag="IYA83_002705"
     CDS             61..414
                     /locus_tag="IYA83_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588074.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
BASE COUNT          287 a          125 c          116 g          198 t
ORIGIN      
        1 ctaatgtaaa aggcttttac aagtttggtt taactcaata aatcagcgaa ggagatcaac
       61 atgaataagg gaaaatcaga gcgttatgta gttataatca atagagaaaa ggttagcgag
      121 ttacctttag cagagtttct tgaatccgaa tatgaaacac taggcgttaa atactctcag
      181 acgatcaaaa aagctttgaa cctatatcgg cgtgtaaaga acaatgaagt tacaattatt
      241 gaagcaggca acgatagaaa cgatagcaaa gtagcagaag taaagaagaa accaactgat
      301 cataacccta ttcaacaaga gcatccttca acggttacat ccgatcattc agcaaagaaa
      361 gaaaagccaa tgctaaaagg gttcaatgca ccaagtaaca aagacttact ttaaaaataa
      421 aaaggcgagg ttacaatacc tagccttttt attatgtaca aatacaaaca taaacatata
      481 cgtatacatg tatataaaca tgtatacgta tatatccata aacacgtata cgaaatttag
      541 ttaagctata cctaaccgct tattccaata aaaaaagtga tcagctttaa actgatcact
      601 caccaaaaac ttcatcatac aagttatcta tcatttcacc aagtaaagct gaatctgatc
      661 gaattcctaa cttttcgctg attttagata atttctttct ttgtgatggt tgaagcgtga
      721 attgat
//
LOCUS       DACIFS010000183          697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.201, whole genome shotgun sequence.
ACCESSION   DACIFS010000183 DACIFS010000000
VERSION     DACIFS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.201"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(54..629)
                     /locus_tag="IYA83_002706"
     CDS             complement(54..629)
                     /locus_tag="IYA83_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ8588075.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DACIFS010000184          692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.202, whole genome shotgun sequence.
ACCESSION   DACIFS010000184 DACIFS010000000
VERSION     DACIFS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.202"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..605)
                     /locus_tag="IYA83_002707"
     CDS             complement(<1..605)
                     /locus_tag="IYA83_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588076.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          224 a          134 c           99 g          235 t
ORIGIN      
        1 atcaattcgt catatcttcc aaaatcaaca ttgtactcat aaccaagaag tgtcggaatc
       61 aggtcaattt caatttcttt ttcaattgaa cgtttaataa gtttcgtgga tacatacccg
      121 acaaaaaaga gaataatacc cacttcaaac aaatacatgt gtccatttac aaagtgaata
      181 aacgtcaata aaaaatcctt cactcctttt gttgttcttc cagattgttc aatttgtttt
      241 attaaagtcg ctagaattgc atttacaaca attaaatcga ataggctttt gaaaagtttt
      301 cctgctgtta atttttgcct ggtaaattgc atgactaatt gagtaaaaat agacaatagc
      361 atgacaaaaa tcaacgtcat tagcactgta aaaatgtctg atgctgcaat cctattggga
      421 tcatttatca agtaataagc cgttccaaat actccaatcg agtaaacggt taaatcaaga
      481 ataagtcgtg ccccgtaact acaaagtttt cctaatggcc ataattgcaa tacccaaaat
      541 ttctttcgtt tgctatttct tttcagagga agccaggtgt gttcaatcca gtcgtccgat
      601 ttcatttcga tttcttccct aaattctttc ctttaacgtt ttcctttgct actttagcag
      661 cgataggttg ctcaatgaac caatttttta ag
//
LOCUS       DACIFS010000185          675 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.205, whole genome shotgun sequence.
ACCESSION   DACIFS010000185 DACIFS010000000
VERSION     DACIFS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.205"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..372
                     /locus_tag="IYA83_002708"
     CDS             <1..372
                     /locus_tag="IYA83_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HAQ8588077.1"
                     /translation="RTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKL
                     LIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIK
                     NTVKYLEKSITSYLPYVNLYD"
     gene            407..>675
                     /locus_tag="IYA83_002709"
     CDS             407..>675
                     /locus_tag="IYA83_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ8588078.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKK"
BASE COUNT          265 a          109 c          111 g          190 t
ORIGIN      
        1 cgtaccatgt cagttccggt accttataag caagaacaaa gagaccctgc ttatttaaaa
       61 ggcgcagaag ataaaacaga gaaaatgaat caatcgtact tgaaagctat gaatagcaaa
      121 tacactaaat tactaatcaa aaactttttg atcagttctg ccgacttatg cgatcttaaa
      181 cttatgagcc gacttcagaa taaagtttat ccgctttatg atcgtttatc caaagataga
      241 ggatttgaat cagttgtcaa tcacctatct tacgttgcta ctcataagga ggattacaca
      301 ataaaaaata ctgttaaata tctcgaaaaa tcaattacga gttatttgcc ttatgtgaac
      361 ctttatgatt aacaactgaa aaaatgcgtg aaggaaggaa aaacttttga aaaaagatga
      421 actgacaaag tacaatgagg agttcttagc aacaattgac atgaaaatgg atttaaagtc
      481 gtatgaactt tatcaattgc tgttcagtta tatctcaccc acaaagctaa agaaaacagg
      541 cgtagtagat ttacctgtta aagcgattat taattacatg gatgttgatc aaaatcttga
      601 aacggaacta aaaaccatag ttaacacttt gcaaagacaa gcagtttatg aaatttcgaa
      661 tctagacaaa aaata
//
LOCUS       DACIFS010000186          650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.207, whole genome shotgun sequence.
ACCESSION   DACIFS010000186 DACIFS010000000
VERSION     DACIFS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.207"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            94..>650
                     /locus_tag="IYA83_002710"
     CDS             94..>650
                     /locus_tag="IYA83_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ8588079.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMM"
BASE COUNT          202 a          126 c          121 g          201 t
ORIGIN      
        1 tcaccaacat cttttttgta taaaaaaata tagaaaagac accctgaaat cctttagaat
       61 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
      121 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      181 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      241 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      301 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      361 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      421 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      481 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      541 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      601 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga
//
LOCUS       DACIFS010000187          591 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.209, whole genome shotgun sequence.
ACCESSION   DACIFS010000187 DACIFS010000000
VERSION     DACIFS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.209"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(251..>591)
                     /locus_tag="IYA83_002711"
     CDS             complement(251..>591)
                     /locus_tag="IYA83_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588080.1"
                     /translation="EALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTN
                     TEQEPLRAISNLFEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFE
                     QTKTKKKNKE"
BASE COUNT          177 a          116 c           81 g          217 t
ORIGIN      
        1 tacccttccc aaagaatctg gagcataact attattaact cccgatatac caaattgttt
       61 atcgttactt ccaaaaatct tagtggcatt aaacacaata aaggttacaa tcacaattcc
      121 aatgcctatg ttacgcataa tcggattatt tgtttgaaat tgaccaataa ttatagcgat
      181 tgccccaaaa aaagcaagaa ttaaacataa ataacctaac agggttaata gcatgattca
      241 tcacaacttt ctattcttta tttttctttt tggttttcgt ttgttcaaac tcatgctcat
      301 ttagataagt aatcaataaa tctcgcatgg atttgctatc cttacttagc gtgtcaaagg
      361 cttcttgtaa gtcagcatcc ttttctactg cgttaaacca atgcttcgtt ccaaattcaa
      421 aaagattact aattgctctt aacggttctt gctcagtatt cgtatttagc cattcaaata
      481 ttcgttgttg agctaatcct aatttgcttt cgtcttcgtt atttgtacgt gcttcataga
      541 ttttagtgtg tatatcatca aaatagtgga ttcgttctct tagcgcttca c
//
LOCUS       DACIFS010000188          529 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.213, whole genome shotgun sequence.
ACCESSION   DACIFS010000188 DACIFS010000000
VERSION     DACIFS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.213"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>529
                     /locus_tag="IYA83_002712"
     CDS             <1..>529
                     /locus_tag="IYA83_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ8588081.1"
                     /translation="NKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGH
                     LEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSI
                     TFDCGKEFSNWKQISN"
BASE COUNT          176 a           91 c          107 g          155 t
ORIGIN      
        1 aacaaatcaa actgtggtag acgcccgctt gttttacctg aggaacaatc agaatatatt
       61 caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc agcgtttcct
      121 attcgttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt taatccttct
      181 gacttaccga tgaaaggcaa gcgtaaacca aatggacatc aagaaagacg tggaaaacaa
      241 gctttccgtc gctctattca tgaacgtgaa aaagattata gccaattctc aaatgagttt
      301 ggtcaccttg aaggtgacac tatcgtaggt ttgaaacata aaagtgctgt aattacctta
      361 gttgagcgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca agcgattgat
      421 attgaaaaca aattaaatca ttggtttgaa tctgtaccga aaaatctatt caaatccatc
      481 acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatg
//
LOCUS       DACIFS010000189          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.214, whole genome shotgun sequence.
ACCESSION   DACIFS010000189 DACIFS010000000
VERSION     DACIFS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.214"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(5..90)
                     /locus_tag="IYA83_002713"
     tRNA            complement(5..90)
                     /locus_tag="IYA83_002713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IYA83_002714"
     tRNA            complement(107..178)
                     /locus_tag="IYA83_002714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IYA83_002715"
     tRNA            complement(190..262)
                     /locus_tag="IYA83_002715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IYA83_002716"
     tRNA            complement(280..361)
                     /locus_tag="IYA83_002716"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IYA83_002717"
     tRNA            complement(369..441)
                     /locus_tag="IYA83_002717"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IYA83_002718"
     tRNA            complement(445..517)
                     /locus_tag="IYA83_002718"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACIFS010000190          510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.215, whole genome shotgun sequence.
ACCESSION   DACIFS010000190 DACIFS010000000
VERSION     DACIFS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.215"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..434)
                     /locus_tag="IYA83_002719"
     CDS             complement(<1..434)
                     /locus_tag="IYA83_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8588082.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DACIFS010000191          490 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.217, whole genome shotgun sequence.
ACCESSION   DACIFS010000191 DACIFS010000000
VERSION     DACIFS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.217"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..415)
                     /locus_tag="IYA83_002720"
     CDS             complement(<1..415)
                     /locus_tag="IYA83_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588083.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          187 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacata
//
LOCUS       DACIFS010000192          476 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.218, whole genome shotgun sequence.
ACCESSION   DACIFS010000192 DACIFS010000000
VERSION     DACIFS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.218"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            22..>476
                     /locus_tag="IYA83_002721"
     CDS             22..>476
                     /locus_tag="IYA83_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ8588084.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DACIFS010000193          473 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.219, whole genome shotgun sequence.
ACCESSION   DACIFS010000193 DACIFS010000000
VERSION     DACIFS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.219"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..>473
                     /locus_tag="IYA83_002722"
     CDS             <1..>473
                     /locus_tag="IYA83_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ8588085.1"
                     /translation="IWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVE
                     DACATTDDANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWST
                     SPFNGIYQGQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGY"
BASE COUNT          182 a           71 c          115 g          105 t
ORIGIN      
        1 aatctggaag tacatgaaaa gcaagggtat ggacggtgca aaagatgaaa gtaagatcaa
       61 taaaatatcc ggaggttcaa gtaccggaga tggatcgaat aattcatcaa gttcagcatc
      121 aggaacagta gaagatgcct gtgcaactac ggatgacgct aattctggtt ctggatcaat
      181 tgggaaagaa ggaaaaatta acggtaaaaa aggtcaagta atagcgaata tgacttatga
      241 agaagctttg aaaaaatatg ggaaagcgat cacgctacca aaattcaaag aaccagattg
      301 gtcaacttca ccttttaacg gaatatatca aggtcagtgt acagagttga cgtgggctta
      361 tatgagccaa ttatacgatg ggaatcaacc gactactgga aatggtggtt atgtatggga
      421 aagctacaaa tcggcaggag caaaaataac gaacaaacca atcgtaggtt atg
//
LOCUS       DACIFS010000194          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.denovo.222, whole genome shotgun sequence.
ACCESSION   DACIFS010000194 DACIFS010000000
VERSION     DACIFS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.denovo.222"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(20..91)
                     /locus_tag="IYA83_002723"
     tRNA            complement(20..91)
                     /locus_tag="IYA83_002723"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IYA83_002724"
     tRNA            complement(113..200)
                     /locus_tag="IYA83_002724"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IYA83_002725"
     tRNA            complement(225..298)
                     /locus_tag="IYA83_002725"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IYA83_002726"
     tRNA            complement(312..382)
                     /locus_tag="IYA83_002726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IYA83_002727"
     tRNA            complement(384..459)
                     /locus_tag="IYA83_002727"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACIFS010000195         7959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.089, whole genome shotgun sequence.
ACCESSION   DACIFS010000195 DACIFS010000000
VERSION     DACIFS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.089"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            128..1564
                     /locus_tag="IYA83_002728"
     CDS             128..1564
                     /locus_tag="IYA83_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ8588086.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IYA83_002729"
     CDS             1753..4200
                     /locus_tag="IYA83_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ8588087.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IYA83_002730"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IYA83_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ8588088.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IYA83_002731"
     CDS             complement(5690..6793)
                     /locus_tag="IYA83_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ8588089.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IYA83_002732"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IYA83_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ8588090.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACIFS010000196         7780 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.090, whole genome shotgun sequence.
ACCESSION   DACIFS010000196 DACIFS010000000
VERSION     DACIFS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.090"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            245..1213
                     /locus_tag="IYA83_002733"
     CDS             245..1213
                     /locus_tag="IYA83_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ8588091.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IYA83_002734"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IYA83_002734"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ8588092.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IYA83_002735"
     CDS             2243..2851
                     /locus_tag="IYA83_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ8588093.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="IYA83_002736"
     CDS             3279..4190
                     /locus_tag="IYA83_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ8588094.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ"
                     /locus_tag="IYA83_002737"
     CDS             4343..4828
                     /gene="vanZ"
                     /locus_tag="IYA83_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ8588095.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="IYA83_002738"
     CDS             complement(5115..5444)
                     /locus_tag="IYA83_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8588096.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="IYA83_002739"
     CDS             5749..6078
                     /locus_tag="IYA83_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ8588097.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="IYA83_002740"
     CDS             6068..6355
                     /locus_tag="IYA83_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ8588098.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="IYA83_002741"
     CDS             complement(6634..7197)
                     /locus_tag="IYA83_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8588099.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1298 c         1546 g         2369 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctaa
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DACIFS010000197         7640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.093, whole genome shotgun sequence.
ACCESSION   DACIFS010000197 DACIFS010000000
VERSION     DACIFS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.093"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(101..544)
                     /locus_tag="IYA83_002742"
     CDS             complement(101..544)
                     /locus_tag="IYA83_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ8588100.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKIAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="IYA83_002743"
     CDS             complement(739..2079)
                     /locus_tag="IYA83_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ8588101.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="IYA83_002744"
     CDS             2361..3257
                     /locus_tag="IYA83_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ8588102.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="IYA83_002745"
     CDS             complement(3400..4341)
                     /locus_tag="IYA83_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ8588103.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="IYA83_002746"
     CDS             4467..5588
                     /locus_tag="IYA83_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ8588104.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="lsa"
                     /locus_tag="IYA83_002747"
     CDS             5846..7348
                     /gene="lsa"
                     /locus_tag="IYA83_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ8588105.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..>7640
                     /locus_tag="IYA83_002748"
     CDS             7415..>7640
                     /locus_tag="IYA83_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ8588106.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMT"
BASE COUNT         2558 a         1428 c         1511 g         2143 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctatttttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg
//
LOCUS       DACIFS010000198         6452 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.100, whole genome shotgun sequence.
ACCESSION   DACIFS010000198 DACIFS010000000
VERSION     DACIFS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.100"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..238
                     /locus_tag="IYA83_002749"
     CDS             <1..238
                     /locus_tag="IYA83_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ8588107.1"
                     /translation="LITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGEN
                     AIVAANATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            305..1042
                     /locus_tag="IYA83_002750"
     CDS             305..1042
                     /locus_tag="IYA83_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ8588108.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1273..2541
                     /locus_tag="IYA83_002751"
     CDS             1273..2541
                     /locus_tag="IYA83_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ8588109.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3028..3492
                     /locus_tag="IYA83_002752"
     CDS             3028..3492
                     /locus_tag="IYA83_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ8588110.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3476..4105
                     /locus_tag="IYA83_002753"
     CDS             3476..4105
                     /locus_tag="IYA83_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ8588111.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            4459..5937
                     /gene="msr"
                     /locus_tag="IYA83_002754"
     CDS             4459..5937
                     /gene="msr"
                     /locus_tag="IYA83_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ8588112.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            5984..6430
                     /locus_tag="IYA83_002755"
     CDS             5984..6430
                     /locus_tag="IYA83_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ8588113.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT         2199 a         1127 c         1322 g         1804 t
ORIGIN      
        1 cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt cagtaaagcc
       61 aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc caggcgtaac
      121 gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa
      181 taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac
      241 agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac aggaggagaa
      301 tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg
      361 tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag ctgcacgccg
      421 agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat atattcaaac
      481 agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga tggaaaagtt
      541 cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc cattagttga
      601 aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg tgttaaatgg
      661 aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca tttcaactga
      721 ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa caaaatttgc
      781 tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca tcaaatctac
      841 gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg acaaaaaggc
      901 tgctcttgaa cttcatgaag cgcaaaaaga atggggattg acctccgaag atatagcttc
      961 tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagtaaca tgattatccg
     1021 tccgacgaca caggaagtat aatccataac ggataatatc caaagagaat tacttgttaa
     1081 atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt cttcatttac
     1141 gtctttttat gtagaaaaag caaatttatc aaaaagaaac aaatgttatt taaatatgga
     1201 cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt cataattcaa
     1261 aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt aggaaatcta
     1321 gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc aactcagaca
     1381 tcgagttctt cagaaacagg aattgaaaac gatacagaga gtactttgca gtcagctact
     1441 gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga acaaacacaa
     1501 accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga agaaatcacg
     1561 aaccaattga cagatgggca aacattgaca catacccatc aagcagattt gtctgaattc
     1621 gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca acaagcgttc
     1681 attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt atatgcatca
     1741 gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt ggcttctgcc
     1801 cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt tacgatgcct
     1861 acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag aaaatatcct
     1921 tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc ttttcagcca
     1981 ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa agatgctacg
     2041 gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt gaacaatgta
     2101 atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc tccgatgtat
     2161 cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg agaaaaaaat
     2221 tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc aaacagcgga
     2281 caacctgtct accgtttata caatccttac agtggagacc atcattacac aatggcacaa
     2341 tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag tttttattct
     2401 ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg tacccatcac
     2461 tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag atatgaagga
     2521 ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa
     2581 gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta ttaccgcaga
     2641 tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg tcataacttt
     2701 tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag
     2761 gcgatttcgg aaatttttgg cggtgtaaaa ttttctactg cagaagagct atcttctata
     2821 gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt
     2881 tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac
     2941 acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac
     3001 gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat
     3061 caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt
     3121 cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt
     3181 ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa
     3241 gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg
     3301 ctaacagaaa aagcccagaa gaaaatcgag cattgtgaaa tggaccataa agatacaggt
     3361 tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat
     3421 aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa taataatgga
     3481 acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga
     3541 aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc
     3601 ttcttctgtc aatatgcaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga
     3661 aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt
     3721 catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctata accaagctta
     3781 cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta
     3841 ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc
     3901 ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg
     3961 attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt
     4021 tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc
     4081 agaaaagatc actacgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt
     4141 ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg
     4201 aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa
     4261 caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat
     4321 gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag
     4381 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
     4441 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
     4501 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
     4561 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
     4621 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
     4681 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
     4741 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
     4801 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
     4861 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
     4921 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
     4981 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
     5041 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
     5101 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
     5161 aagcaacaaa tcagaccgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
     5221 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
     5281 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
     5341 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
     5401 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
     5461 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
     5521 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
     5581 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
     5641 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
     5701 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
     5761 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
     5821 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
     5881 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
     5941 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
     6001 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
     6061 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
     6121 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
     6181 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
     6241 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
     6301 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
     6361 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
     6421 taatgaatga tggaacactg ttatttgaaa aa
//
LOCUS       DACIFS010000199         4944 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.109, whole genome shotgun sequence.
ACCESSION   DACIFS010000199 DACIFS010000000
VERSION     DACIFS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.109"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            21..1061
                     /locus_tag="IYA83_002756"
     CDS             21..1061
                     /locus_tag="IYA83_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ8588114.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1159..1347
                     /locus_tag="IYA83_002757"
     CDS             1159..1347
                     /locus_tag="IYA83_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8588115.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1405..1758)
                     /locus_tag="IYA83_002758"
     CDS             complement(1405..1758)
                     /locus_tag="IYA83_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8588116.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2289..2711
                     /locus_tag="IYA83_002759"
     CDS             2289..2711
                     /locus_tag="IYA83_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ8588117.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2708..2938
                     /locus_tag="IYA83_002760"
     CDS             2708..2938
                     /locus_tag="IYA83_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ8588118.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3436..3636
                     /locus_tag="IYA83_002761"
     CDS             3436..3636
                     /locus_tag="IYA83_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ8588119.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3663..4856
                     /locus_tag="IYA83_002762"
     CDS             3663..4856
                     /locus_tag="IYA83_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ8588120.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1705 a          798 c          912 g         1529 t
ORIGIN      
        1 aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata
       61 gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc
      121 ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac
      181 aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg
      241 aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta
      301 tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt
      361 atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa
      421 atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt
      481 tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg
      541 gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac
      601 aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact
      661 atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag
      721 cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac
      781 cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa
      841 atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc
      901 aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc
      961 taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc
     1021 ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata
     1081 taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt
     1141 ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat
     1201 aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc
     1261 aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag
     1321 ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct
     1381 ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt
     1441 tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac
     1501 ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga
     1561 tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt
     1621 ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt
     1681 gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt
     1741 atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct atataaccat
     1801 aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca ctgtggaatt
     1861 tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt acctatacaa
     1921 ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt aatggaggga
     1981 aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt cttatcaaaa
     2041 aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca gattacatag
     2101 atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt ttttggtcac
     2161 ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg
     2221 ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac agaaaggggg
     2281 tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt gactatatct
     2341 gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac agactatcag
     2401 caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt tctactgttg
     2461 atacgtacgt cactgatttc caactattta aattatttgg attaagaatc aatgttgaaa
     2521 gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac attattttac
     2581 ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat ttgaatcgtt
     2641 cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc atgaaggagt
     2701 gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa aggcgacaga
     2761 tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata cctcaaaacg
     2821 ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg atgaaaccct
     2881 acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa ttaaatagtc
     2941 ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt
     3001 attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag ccaaacgttc
     3061 tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct gatgaactaa
     3121 cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa gtaatcttta
     3181 gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata gccatagtcc
     3241 gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc agaagtgaaa
     3301 ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt gtcagtgtat
     3361 tattataaaa ctatttgata taattatagg tgaatattgg agttttccta gcctataatt
     3421 gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct aggatatgga
     3481 acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac aaaagggcat
     3541 atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga agaaattcta
     3601 ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa
     3661 ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc
     3721 taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc actactaaaa
     3781 aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa ttaagtgaga
     3841 ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca tggtataaga
     3901 cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag catttctctt
     3961 attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat tggcaactaa
     4021 aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta ttttcagttg
     4081 ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag attattggca
     4141 atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt gaaaaagtaa
     4201 tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct ctgtggtttt
     4261 tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa gatattgatt
     4321 ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa tcgaattata
     4381 gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat aaatgcacat
     4441 taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca gactttgtaa
     4501 tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt gaacgctatg
     4561 ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct catgcttcct
     4621 tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga cacgaagata
     4681 ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc gaggtagcta
     4741 acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat acttcaccaa
     4801 agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt
     4861 gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttcg gctctataat
     4921 ttatgggact gatgaatcag aatt
//
LOCUS       DACIFS010000200         4277 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.117, whole genome shotgun sequence.
ACCESSION   DACIFS010000200 DACIFS010000000
VERSION     DACIFS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.117"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..363
                     /locus_tag="IYA83_002763"
     CDS             <1..363
                     /locus_tag="IYA83_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ8588121.1"
                     /translation="NFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFAC
                     NNAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIV
                     SEINNFLNEESSDERKQI"
     gene            344..1078
                     /locus_tag="IYA83_002764"
     CDS             344..1078
                     /locus_tag="IYA83_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ8588122.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1111..2019
                     /locus_tag="IYA83_002765"
     CDS             1111..2019
                     /locus_tag="IYA83_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAQ8588123.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2016..2558
                     /gene="sat4"
                     /locus_tag="IYA83_002766"
     CDS             2016..2558
                     /gene="sat4"
                     /locus_tag="IYA83_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ8588124.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2651..3445
                     /locus_tag="IYA83_002767"
                     /note="aph(3')-IIIa"
     CDS             2651..3445
                     /locus_tag="IYA83_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ8588125.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(3553..3741)
                     /locus_tag="IYA83_002768"
                     /pseudo
     CDS             complement(3553..3741)
                     /locus_tag="IYA83_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3721..4263
                     /locus_tag="IYA83_002769"
     CDS             3721..4263
                     /locus_tag="IYA83_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ8588126.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         1464 a          643 c          975 g         1195 t
ORIGIN      
        1 aactttttta tgtttgaagc agggttctct ttaatgtttg taaaagcaaa ttcgggaaca
       61 gacgataaat attatattgc gggtcatgtt tttcgtatag tttcatgttt aaatcaagtg
      121 ttatttgcat gtaataatgc ttattgtatc aacgaaaaga aagctataaa actgcttgaa
      181 acttttgaac ataaacctga aaaatatacc gagaaggtaa atcatatttt tgaagtactc
      241 ggtatctcac tttttgaatg ctacgacatg accgagaagc tttataatga agtgaatgaa
      301 attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata
      361 tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc
      421 tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt
      481 gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc
      541 ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca
      601 ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag
      661 ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat
      721 aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc
      781 tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca
      841 tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa
      901 ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt
      961 tataataaat cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat
     1021 ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata
     1081 atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta
     1141 gtactttctt tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc
     1201 gcaaatatta atatacctaa agatgaattt caggattatg atattacata ttttgtaagt
     1261 gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg
     1321 atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt
     1381 atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat
     1441 aattacctaa agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa
     1501 agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat
     1561 gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt
     1621 aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga
     1681 atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat
     1741 aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg
     1801 atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg
     1861 gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata
     1921 acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc
     1981 acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc
     2041 acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga
     2101 ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa
     2161 gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca
     2221 atactgataa gataatatat ctttactacc aagacgataa atgcgtcgga aaagttaaac
     2281 tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat cgccgtatgt aaggatttca
     2341 gggggcaagg cataggcagc gcgcttatca atatatctat agaatgggca aagcataaaa
     2401 acttgcatgg actaatgctt gaaacccagg acaataacct tatagcttgt aaattctatc
     2461 ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
     2521 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
     2581 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
     2641 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
     2701 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
     2761 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
     2821 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
     2881 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
     2941 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
     3001 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
     3061 cagccgatta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
     3121 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
     3181 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
     3241 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
     3301 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
     3361 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
     3421 tattttactg gatgaattgt tttagtacct agatttagat gtctaaaaag ctttaactac
     3481 aagcttttta gacatctaat cttttctgaa gtacatccgc aactgtccat actctgatgt
     3541 tttatatctt ttctaaaagt tcgctagata gagttctata ttagagatgt aaagagtttt
     3601 ggtgaagagg tttgtgagta tggcttttat aaccagggtg caatgagaag cactaacagc
     3661 agctagctta agaacgcata ggatatactt tttatggaag tcacagagga ggaataaaat
     3721 ttgaacagga ttaacagagt aacctctatt cttattcagc tgcaatcaaa aaaaataatt
     3781 cctgccaaag aaattgcaca gcgatttaat ataagcttaa ggacagttta cagagatatc
     3841 cggacacttg aagaagccgg aataccaatt ggatctgagg ccggaaaagg atattttctt
     3901 gtagagggct tcctacttcc gccggtaatg tttacagcgg cggaagtggg tgcattgatt
     3961 acagcaggga aatttttaaa ttgccatgga gatgaatcat ttataaagga ttttgattca
     4021 gctatgtata aaatcaaatc tattttgaag catggtgaaa aaaactatgc acaggagttg
     4081 gagaacagta ttaacgtgta cagcacatct ggacagaaaa atacattggc ggataacgtt
     4141 attgcagcaa ttcagactgc aatctgcaat aaaagggtaa tatcaattca atatcctgca
     4201 tcaggagggc aagaaccgga aagcagaatg atagagccgg ttctgttgca aagttttaaa
     4261 taaagaataa aatccct
//
LOCUS       DACIFS010000201         3720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.121, whole genome shotgun sequence.
ACCESSION   DACIFS010000201 DACIFS010000000
VERSION     DACIFS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.121"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            554..640
                     /locus_tag="IYA83_002770"
                     /pseudo
     CDS             554..640
                     /locus_tag="IYA83_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance protein"
     gene            641..2581
                     /gene="tet(S)"
                     /locus_tag="IYA83_002771"
     CDS             641..2581
                     /gene="tet(S)"
                     /locus_tag="IYA83_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HAQ8588127.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2669..2836
                     /locus_tag="IYA83_002772"
     CDS             2669..2836
                     /locus_tag="IYA83_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ8588128.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3350..3706)
                     /locus_tag="IYA83_002773"
     CDS             complement(3350..3706)
                     /locus_tag="IYA83_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ8588129.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1327 a          577 c          618 g         1198 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaacccttt taatgatatt ttccaagtca
       61 aacttaatat taacaattca aaactattgg aaccactgca aaatattttg ttaagcagta
      121 tatatttcaa aaattatacg taatatattt gtagaaaggt tgtggacagt tgtcctattg
      181 acctgaaaag taatataata gctggatgaa taaagaatcc cttatgaact tgtaaatcgt
      241 tattaaatcg ctgaattttg atagccattc tgaagagtta tcttcaacta ctacactaac
      301 ttccattaat ttctcattaa tttccctttc gttcaaaaaa tatggttggc tttacaagca
      361 ttctatcata tgatagaatt aagataacat tcaaaaggag ataataaaaa tgcggcaagg
      421 tattcttaaa taaactgtca attagatagc gggaataatt aattaagcat ccttatttaa
      481 aataaggact ggttttttgt gcccagttta agaatacttt tatcatttta tcataaagca
      541 tctgtggcta tcagtgtgta gtatacagct atttttgtat taaaatccca gtgataagag
      601 tatttatcac tgggattttt tctgcttttt gagcttttga atggaggaaa taaaattgaa
      661 aattattaat atcggtatct tagcacatgt tgatgcagga aaaactactt tgacagaaag
      721 cttactatac agtagcggag caattaaaga gttaggaagt gtagatagcg gtacaacgaa
      781 aacggatact atgtttttgg aacgccagag aggtattact attcagaccg caataacatc
      841 ttttcaacgg gaaaatgtta aagtaaatat tgtagatact cctggacaca tggatttttt
      901 ggcagatgta taccgttcat tatctgtttt ggatggagct attttgctaa tctctgcaaa
      961 agatggagta cagtcacaaa ctcgtatact attccatgca cttagaaaga tgaacatacc
     1021 tataatattt tttattaaca aaattgatca aaatggaata aatttgccag atgtttatca
     1081 agatattaag gacaaacttt ctgacgacat cataattaag cagactgtga atctaaattt
     1141 gaaaccttat gtaatagatt atactgaacc agaacaatgg gagacagtaa ttgtgggaaa
     1201 tgattattta ttagaaaaat ataccattgg gaaaacattg aatattgcag aacttgaaaa
     1261 ggaggaaaac gaaagaattc aaagttgctc cttatatcct gtttatcacg gaagtgcaaa
     1321 gaataatatt ggaattaaac aacttataga ggtaattact agcaaattat tttcacccac
     1381 acaactcaat tcagataaac tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg
     1441 tcaacggctt gtctatgtac gtctttatag tggaacgcta catttgcgag actcagtcaa
     1501 tatatcagaa aaggaaaaaa taaaagttac agaaatgtat acttcaataa atggagaatt
     1561 acgccagata gataaggcag agcctggtga gattattatt ttaaaaaatg agcttttaaa
     1621 actaaataac gtacttggag ataaaaaaag attaccacat agagaaattc ttgagaatcc
     1681 tcttcctatg ttacaaacaa caattgaacc atgtaaatca gtacaaagag aaaagttact
     1741 agatgcactt tttgaaatat ccgatagtga tccccttcta caatattatg tagatacagt
     1801 aactcacgaa attgtgctat cttttttagg tgaggtccaa atggaggtaa cttgtactct
     1861 gattcaagaa aaatatcata ttgagataga aacaagaaaa ccaactgtca tttatatgga
     1921 aagaccatta aaaaaatctg aatttaccat tgatatcgaa gtacctccaa atcctttctg
     1981 ggcttctatt ggtttatctg taacaccact tcctttgggt agtggcattc agtatgagag
     2041 cctggtttct ctaggttatt taaatcaatc atttcaaaat gcagttatgg aaggtatacg
     2101 ctatgggtgt gaacaaggat tgtacggttg gaaattaaca gactgtaaga tctgttttaa
     2161 gtatggtcta tattacagcc ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat
     2221 tgtactagag caggctttta gaaagagtgg tacagagtta ttagagccat atcttagctt
     2281 cgaaatttat gtaccacaag aatatctttc gagagcatat aatgatgctt ccaaatattg
     2341 tgcaaatatt ttaaatacta agttaaaagg taacgaggtc attctcattg gtgaaattcc
     2401 agcccgttgt attcaagagt atcgaaacag tttaactttc tttacaaatg gacgcagtgt
     2461 ctgtttaaca gagttaaaag gttatcaggt tactaacatt aagtctgctt tccaaccacg
     2521 tcgtccaaat aatagaatag acaaagtaag gcatatgttt aataaaatca acttacattg
     2581 atagtgaact aaagagaaat ttttaaaatg aatattgagt tgttaattag aacaggagga
     2641 tatagatatt gtaaatacta ggtggatact gtgccctata tgtggcaata agacccgttt
     2701 aaaaatgagg gaagatactg aattaaaaaa tttccctcta ttttgtccta aatgcagaca
     2761 agaaatttta attgaaataa cgaagtttag aataactgta attacagagc cagacgcaaa
     2821 gacgcagagc cgataattcg agatatcaaa atctcatttt atcggctttt tcttttctat
     2881 gcataccatt gagcttagtt cttgatatcc tttataagaa aataagtgta ctgatttttt
     2941 ttgcagtagc tgtattacag tagacatccc tcaagcataa aaagtataca attaaaattc
     3001 aatacttgat tattgatgaa gtacagagtt actcgtcatt tcaaaactag tatatcagtc
     3061 aagtttttcc taaggaacaa aaacgaactt cgctatggga atcaaaaatt tttagtcgga
     3121 tgaagtgata tgcaataaat ccagaaaatt ttcccaacaa caaaaaatga actgtacttt
     3181 gcagcgataa gagtaaccaa agaactattt atcattcgct ctagttccta agtgtaacaa
     3241 agataaattc attttctatt tatgattaac aaaaatcaga aaaaattcat tacatctttg
     3301 taatcaatct ttcctgatag tattaattaa tccattgtgt attattgaac tagttaaatt
     3361 tacatttttc tccgtaaatt gcttgaatca ctttcccaat cgtcactaat tcatcttcat
     3421 ccagacaatc caactgtgct tctacttgaa gacgtttgga gcttttatga ctgttttctt
     3481 cccctaaaat aaatccatcc aaagaaatat ttagcaaagt cactaaatca tatacaacat
     3541 gaaaacttgg ctgttgtcct ttattttcaa tgttcgtcaa atagcgtggc gcaatctgga
     3601 tcattgatcc tacctgctca cgagtcaatc ctttactaag acgggcttct ttaatcgcaa
     3661 gacccaatgg tttgaaatca aaatttgata gagtagtttg ccatattctt gcacccaacg
//
LOCUS       DACIFS010000202         3650 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.123, whole genome shotgun sequence.
ACCESSION   DACIFS010000202 DACIFS010000000
VERSION     DACIFS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.123"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            complement(<1..151)
                     /locus_tag="IYA83_002774"
     CDS             complement(<1..151)
                     /locus_tag="IYA83_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ8588130.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSIN"
     gene            381..878
                     /gene="dfrG"
                     /locus_tag="IYA83_002775"
     CDS             381..878
                     /gene="dfrG"
                     /locus_tag="IYA83_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ8588131.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(950..2902)
                     /locus_tag="IYA83_002776"
     CDS             complement(950..2902)
                     /locus_tag="IYA83_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ8588132.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2909..3205)
                     /locus_tag="IYA83_002777"
     CDS             complement(2909..3205)
                     /locus_tag="IYA83_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ8588133.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3380..>3650)
                     /locus_tag="IYA83_002778"
     CDS             complement(3380..>3650)
                     /locus_tag="IYA83_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ8588134.1"
                     /translation="IEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAK
                     SNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
BASE COUNT         1125 a          632 c          590 g         1303 t
ORIGIN      
        1 catttattga agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg
       61 aatctcgtaa atgtagtact ccactataaa ggcgtatata tgcaagtaag tagttcagtt
      121 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
      181 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
      241 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
      301 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
      361 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
      421 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
      481 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
      541 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
      601 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
      661 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
      721 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
      781 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
      841 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
      901 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
      961 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1021 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     1081 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     1141 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     1201 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     1261 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     1321 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     1381 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     1441 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     1501 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     1561 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     1621 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     1681 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     1741 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     1801 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     1861 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     1921 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     1981 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     2041 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     2101 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     2161 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     2221 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     2281 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     2341 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     2401 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     2461 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     2521 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     2581 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     2641 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     2701 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     2761 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     2821 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     2881 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     2941 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3001 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     3061 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     3121 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     3181 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     3241 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     3301 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     3361 tactccaaaa ctgaactact taatatgcaa gacgttgtct tttttttgta tattcaattt
     3421 tgaaaacatt tccgcaaagt tcagacggac ctcgatgtgt tgatgaataa aatttattag
     3481 taataacttc tataaggtta tcaatcccta tattactttt tgcacttcca tgataaagag
     3541 ggaacagaga acaattctga aatcttatgc tttcctcttg ttcgagttcc aatgcttcta
     3601 atgatttacc ggacatatat ttctctaaaa ggtcatcgtt tccctctatt
//
LOCUS       DACIFS010000203         3243 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.126, whole genome shotgun sequence.
ACCESSION   DACIFS010000203 DACIFS010000000
VERSION     DACIFS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.126"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            110..193
                     /locus_tag="IYA83_002779"
     CDS             110..193
                     /locus_tag="IYA83_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ8588135.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="IYA83_002780"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="IYA83_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ8588136.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1225..1485
                     /locus_tag="IYA83_002781"
                     /pseudo
     CDS             1225..1485
                     /locus_tag="IYA83_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1648..2313
                     /locus_tag="IYA83_002782"
                     /pseudo
     CDS             1648..2313
                     /locus_tag="IYA83_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2349..2594
                     /locus_tag="IYA83_002783"
     CDS             2349..2594
                     /locus_tag="IYA83_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ8588137.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2697..>3243
                     /locus_tag="IYA83_002784"
     CDS             2697..>3243
                     /locus_tag="IYA83_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ8588138.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMF"
BASE COUNT         1166 a          491 c          599 g          987 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tttgtgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttac agatgtaata
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt
     1081 ggaaagttac acgttactaa agggaatgga gataaattat tagatatact actgacagct
     1141 tccaagaagc taaagaggtc cctagcgcct acggggaatt tgtatcgata aggggtacaa
     1201 attcccacta agcgctcggg accccttgta ggaaaatgtc ctaagtgtgg caacaatatt
     1261 gtattaaaaa aatcgtttta tggttgttca aattatcctg aatgtaagtt tactttagct
     1321 gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa aagaattact ggagggaaaa
     1381 gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc cctacaatgc cgttgtaaaa
     1441 attggggaaa agggatatat tgattttatc tctttttcaa aataaacatg gggctgaccc
     1501 aaaagtagct cctatatgaa aaatagaggt ctttccattt agatagtagt ggaaagacct
     1561 ctatttatct tttgaaagtg aatacaaaaa taaagcacac tctgctataa tataggtgat
     1621 taatccattt gagagaggtg ctttatcatg tatcaaaatt ataccataaa ccaactttgt
     1681 ttgcctatag atcttgaaat taaattagaa gaaaatgatt ttgctcatgc cattgtgcag
     1741 tttgttgact ctattcccga tgaagtcttt cttccttatt atcaatctat gggtagaccc
     1801 caatatcatc ctcgtatgct actttccatt attctttgtg cctatattca aggggtttat
     1861 tcgggccgcc aaatacaaaa tatgctgata gatagtattc ggatgcgcta cttatctcaa
     1921 gagcaattcc ctaactttcg tactattaat cgttttcgcg ttcatccgat tatgaacgat
     1981 attctagatc attcatttgt tcaatttcgt gagctactgg ttcaaagtgg cttaatcagt
     2041 ggcagtgctc tatacattga tggtacaaaa attgaagcgg atgcgaataa atactctttc
     2101 gtttggaaaa aaagtatttt aaagtataag gatagccttg atcaaaaagc ccttgaaaac
     2161 taccaacaaa tggtggaaac aaaaatctta cctgagttaa tagatgattt aaaggatgaa
     2221 ctttctatag aagagattga aaggattcgt caatctttgg aagaaaagga aaatcaatta
     2281 attgaagcga ttgaccaaac cgaaacagta gaagggggct gacccattat taataaggag
     2341 tggacaacat gattgatgta aatgagctat tggaagaagc tattagagaa actgaaaatc
     2401 taaatgatgg cgaagttttt cttgttaaag atttattcaa gggatatgtg gggaatagga
     2461 tacccagaaa ggatcgactt ctgcttggaa cgttattttt aaaccatgtt agcaaaatga
     2521 atggtaattt aaaggctatt gaaaagactt catctaatca gcagaggtat aaaaagacaa
     2581 ttgataagtt ttaactatct tttgtgaaat taaaaaaatt ataaatatgt tgagtagaag
     2641 caaaagattt ggcttggaat ttttacagca gtgaatcgat aaaaggagca tgcttgatgg
     2701 tagataatat tattaaatca gtagcagaga aattatcctc tctgtcttat atagaaggta
     2761 ttgttttagg tggttcacgt gcaaggggca cccatacaga ggattcggat atagatatcg
     2821 gcatctatta caattcagaa tcatttgaca taaatactat taatcaattc gctacaaagc
     2881 tggatgatga gcatagaaat aaccttgttg tacctcccgg agcatggggt gattggatta
     2941 atggcggcgg atggttagtc ataaacgggt atcatgtgga tttaatttta cgtgatatta
     3001 aacgtgtgga acaaataatg aaagatacag agcacggaat tgttactgcc aattatcaga
     3061 ctgggcatcc ccatggttat attagtgcaa tgtatcgagg agaattagcg attagcaaaa
     3121 tactatatgc taagaatgaa agcttatgcg aattaaaaaa acaggcagaa acttatccca
     3181 atgctttgca gaaaagttta gttaactttt ttatgtttga agcagggttc tctttaatgt
     3241 ttg
//
LOCUS       DACIFS010000204         3025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.129, whole genome shotgun sequence.
ACCESSION   DACIFS010000204 DACIFS010000000
VERSION     DACIFS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.129"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..721
                     /locus_tag="IYA83_002785"
     CDS             <1..721
                     /locus_tag="IYA83_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ8588139.1"
                     /translation="YTQENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLF
                     PRFMHGTLIGKLSGGEKRRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFP
                     GAVITVSHDRYFLDKVADKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQ
                     SKEPAEKKKLSYMEQKEWETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSE
                     TEAQLEEKMARWEYLSEWVED"
     gene            733..1680
                     /locus_tag="IYA83_002786"
     CDS             733..1680
                     /locus_tag="IYA83_002786"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ8588140.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            1700..2227
                     /locus_tag="IYA83_002787"
     CDS             1700..2227
                     /locus_tag="IYA83_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ8588141.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(2290..2943)
                     /locus_tag="IYA83_002788"
     CDS             complement(2290..2943)
                     /locus_tag="IYA83_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ8588142.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1070 a          538 c          625 g          792 t
ORIGIN      
        1 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
       61 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
      121 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
      181 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
      241 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
      301 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
      361 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
      421 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
      481 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
      541 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
      601 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
      661 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
      721 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
      781 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
      841 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
      901 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
      961 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
     1021 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
     1081 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
     1141 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
     1201 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
     1261 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
     1321 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
     1381 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
     1441 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
     1501 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
     1561 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
     1621 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
     1681 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
     1741 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
     1801 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
     1861 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
     1921 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
     1981 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
     2041 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
     2101 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
     2161 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
     2221 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
     2281 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
     2341 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
     2401 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
     2461 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
     2521 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
     2581 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
     2641 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
     2701 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
     2761 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
     2821 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
     2881 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
     2941 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
     3001 taaaaataga aattgtctta catgt
//
LOCUS       DACIFS010000205         2551 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V1074
            SAMEA5367833-rid9539773.guided.134, whole genome shotgun sequence.
ACCESSION   DACIFS010000205 DACIFS010000000
VERSION     DACIFS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5367833
            Sequence Read Archive: ERR3171846
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000700328.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000700328.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2020 18:06:51
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,791
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,673
            CDSs (with protein)               :: 2,673
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 30 of 63
            Pseudo Genes (incomplete)         :: 30 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5367833-rid9539773.guided.134"
                     /isolate="V1074"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2015"
     gene            <1..459
                     /locus_tag="IYA83_002789"
     CDS             <1..459
                     /locus_tag="IYA83_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ8588143.1"
                     /translation="TLFDSRFKTLQQNLQEDDIIYVTDLTRITRSTQDLFELIDNIRD
                     KKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFK
                     GRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            673..1368
                     /gene="vanR"
                     /locus_tag="IYA83_002790"
     CDS             673..1368
                     /gene="vanR"
                     /locus_tag="IYA83_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ8588144.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1346..2500
                     /locus_tag="IYA83_002791"
     CDS             1346..2500
                     /locus_tag="IYA83_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ8588145.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          880 a          453 c          535 g          683 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc tacaggaaga tgacatcatt
       61 tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat
      121 aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca
      181 gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag
      241 cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag
      301 tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag
      361 ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg
      421 gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg
      481 ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc
      541 gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg
      601 taaatgctta taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa
      661 agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat
      721 ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa
      781 gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt
      841 cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt
      901 atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg
      961 gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag
     1021 ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac
     1081 tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc
     1141 cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt
     1201 agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac
     1261 accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg
     1321 aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta
     1381 ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt
     1441 attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat
     1501 tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat
     1561 acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg
     1621 tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt
     1681 acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca
     1741 aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga
     1801 acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc
     1861 cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc
     1921 aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt
     1981 ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct
     2041 atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa
     2101 acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc
     2161 gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat
     2221 tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag
     2281 catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg
     2341 ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca
     2401 gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct
     2461 tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata taattttggc
     2521 tctttgtcaa atgatgttgg taaagagaaa t
//
